CN113475385A - Breeding method of sterile line of three-line hybrid rice with leaf marking property - Google Patents
Breeding method of sterile line of three-line hybrid rice with leaf marking property Download PDFInfo
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- CN113475385A CN113475385A CN202110816431.5A CN202110816431A CN113475385A CN 113475385 A CN113475385 A CN 113475385A CN 202110816431 A CN202110816431 A CN 202110816431A CN 113475385 A CN113475385 A CN 113475385A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
- A01H1/023—Male sterility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
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- Natural Medicines & Medicinal Plants (AREA)
- Physiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a breeding method of a sterile line of three-line hybrid rice with leaf marking property, which comprises the following steps: s1: carrying out gene marking on the male parent rice plant of the primary sterile line; s2: selecting a primary generation female rice plant, removing pistils, and hybridizing the primary generation female rice plant with a marked primary generation male rice plant; s3: hybridizing the primary generation female rice plant with the primary generation male rice plant to obtain a secondary generation rice plant; s4: selecting normal rice plants with whitened leaves with marking characteristics in the rice plants of the next generation and male plants of the sterile line with marking characteristics for hybridization; s5: obtaining the third generation rice plants, and screening out the male sterile plants with the marking characteristics. The invention adopts a hybridization screening mode, can eliminate normal plants without whitening marks, adopts normal plants with whitening marks to hybridize with male sterile plants with marks, and can effectively improve the cultivation quantity of the male sterile plants.
Description
Technical Field
The invention relates to the technical field of rice cultivation, in particular to a breeding method of a sterile line of three-line hybrid rice with leaf marking property.
Background
The three-line hybrid rice is also called indica hybrid rice, is one of the hybrid rice cultivated in modern times, greatly improves the yield of the rice, gradually increases the planting area of the indica hybrid rice along with the popularization and the popularization of agriculture, and greatly improves the yield of the rice.
The cultivation of indica hybrid rice needs to select male sterile plants, the cultivation of the male sterile plants has certain difficulty, the male sterile plants need to be screened in the cultivation process, the male sterile plants are characterized by thin anther, shrivelled anther and non-cracking anther, the male sterile plants are not beneficial to observation, a large amount of labor cost needs to be invested by workers to comprehensively observe the male anther characters of the rice plants, and the work cost investment is large.
After massive search, the prior art is found, the publication number is CN104026002B, and a rapid cultivation method of a sterile line of three-line hybrid rice is disclosed, which comprises the following steps: when the fertility of the test cross progeny of the new rice variety P and the sterile line A is identified, F1 generated by the hybridization of the new rice variety P and the improved maintainer line B is used as a male parent to hybridize with the male parent to maintain the fertility of the sterile line of the test cross progeny, single plants prone to target characters are selected from F2 and the later generations generated by the hybridization of the new rice variety P and the improved maintainer line B, selected sterile lines generated by the continuous hybridization of the new rice variety P and the test cross progeny of the sterile line A are hybridized in pairs, and genetic components of the selected maintainer line single plants are continuously infiltrated into the sterile line, so that the sterile line is quickly stabilized, and the fertility of the sterile line is improved. The method detects the recovery and preservation relation of the parent in the initial stage, and simultaneously carries out the transformation of the sterile cytoplasm, and also carries out the transformation of the sterile cytoplasm when the segregating generation selects, thereby greatly shortening the breeding period.
In summary, in the existing cultivation process of male sterile plants, normal plants without the marker feature are mixed in the second generation and third generation plants, and the normal plants without the marker feature are not needed in the cultivation process of the male sterile plants, so that the proportion of the male sterile plants is reduced, and the subsequent cultivation and screening of the male sterile plants are not facilitated.
Disclosure of Invention
The invention aims to provide a breeding method of a sterile line of three-line hybrid rice with a leaf marking character, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a breeding method of a sterile line of three-line hybrid rice with leaf marking character comprises the following steps:
s1: carrying out gene marking on the male parent rice plant of the primary sterile line;
s2: selecting a primary generation female rice plant, removing pistils, and hybridizing the primary generation female rice plant with a marked primary generation male rice plant;
s3: hybridizing the primary generation female rice plant with the primary generation male rice plant to obtain a secondary generation rice plant;
s4: selecting normal rice plants with whitened leaves with marking characteristics in the rice plants of the next generation and male plants of the sterile line with marking characteristics for hybridization;
s5: obtaining the third generation rice plants, and screening out the male sterile plants with the marking characteristics.
Preferably, in S1 based on the breeding method:
the recessive gene of the male sterile plant is marked with whitening characteristic by adopting a gene marking method, and the double recessive characteristic of the male sterile plant of the first generation is characterized by whitening of plant leaves.
Preferably, in S2 based on the breeding method:
the plant of the first generation mother selects a normal plant with normal dominant characteristics, and pistils are removed before the stage of stamens blooming, so as to ensure the success rate of pollination.
Preferably, in S3 based on the breeding method:
pollination is carried out on the first generation male parent and the first generation female parent rice plants in an artificial pollination mode, stamen pollen of the first generation female parent is contacted with stigma of pistil of the first generation sterile line, pollination and hybridization of the first generation male parent and the first generation female parent are realized, hybridized seeds are obtained, and the seeds are planted to obtain the second generation plants.
Preferably, in S4 based on the breeding method:
obtaining seeds after the hybridization of the primary female parent and the primary male parent, obtaining secondary rice plants, screening the secondary rice plants, selecting albino leaf normal plants with marked recessive genes and selecting albino leaf male sterile plants with marked recessive genes, and eliminating the albino leaf normal rice plants without marked recessive genes;
and (4) removing pistils from the albino normal plants of the second generation, and hybridizing with male sterile plants of the second generation to obtain seeds of the second generation.
Preferably, in S5 based on the breeding method:
and (3) sowing the second generation seeds to obtain third generation rice plants which are albino leaf normal plants and albino leaf male sterile plants, and eliminating normal rice plants without albino leaves.
Compared with the prior art, the invention has the beneficial effects that: the invention crosses the first generation male sterile plant and the normal plant with the pistil taken out from the first generation, the first generation male sterile plant has the shape of whitening leaf mark, the second generation rice plant is obtained after crossing, in the second generation rice plant, the normal plant without the characteristic of whitening leaf mark is removed, the second generation male sterile plant with the characteristic of whitening leaf mark is selected to be crossed with the normal plant with the characteristic of whitening leaf mark, the normal plant and the male sterile plant both with the characteristic of white leaf mark can be obtained, when the third generation plant is crossed to obtain the third generation plant, the normal plant without the white leaf mark can not appear in the third generation plant, only the normal plant with the characteristic of white leaf mark and the male sterile plant with the white leaf mark can appear, and the cultivation proportion of the male sterile plant can be effectively improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The embodiment provided by the invention comprises the following steps: a breeding method of a sterile line of three-line hybrid rice with leaf marking character comprises the following steps:
s1: carrying out gene marking on the male parent rice plant of the primary sterile line;
s2: selecting a primary generation female rice plant, removing pistils, and hybridizing the primary generation female rice plant with a marked primary generation male rice plant;
s3: hybridizing the primary generation female rice plant with the primary generation male rice plant to obtain a secondary generation rice plant;
s4: selecting normal rice plants with whitened leaves with marking characteristics in the rice plants of the next generation and male plants of the sterile line with marking characteristics for hybridization;
s5: obtaining the third generation rice plants, and screening out the male sterile plants with the marking characteristics.
The method comprises the steps of hybridizing a primary male sterile plant with a normal plant with a pistil taken out of the primary male sterile plant, wherein the primary male sterile plant is in a whitened leaf mark shape, hybridizing to obtain a secondary rice plant, removing normal plants without the whitened leaf mark feature from the secondary rice plant, and hybridizing the secondary male sterile plant with the whitened leaf mark with the normal plant with the whitened leaf mark feature to obtain normal plants and male sterile plants both with a white leaf mark feature.
In S1 based on the breeding method:
the recessive gene of the male sterile plant is marked with whitening characteristic by adopting a gene marking method, and the double recessive characteristic of the male sterile plant of the first generation is characterized by whitening of plant leaves.
In S2 based on the breeding method:
the plant of the first generation mother selects a normal plant with normal dominant characteristics, and pistils are removed before the stage of stamens blooming, so as to ensure the success rate of pollination.
In S3 based on the breeding method:
pollination is carried out on the first generation male parent and the first generation female parent rice plants in an artificial pollination mode, stamen pollen of the first generation female parent is contacted with stigma of pistil of the first generation sterile line, pollination and hybridization of the first generation male parent and the first generation female parent are realized, hybridized seeds are obtained, and the seeds are planted to obtain the second generation plants.
In S4 based on the breeding method:
obtaining seeds after the hybridization of the primary female parent and the primary male parent, obtaining secondary rice plants, screening the secondary rice plants, selecting albino leaf normal plants with marked recessive genes and selecting albino leaf male sterile plants with marked recessive genes, and eliminating the albino leaf normal rice plants without marked recessive genes;
and (4) removing pistils from the albino normal plants of the second generation, and hybridizing with male sterile plants of the second generation to obtain seeds of the second generation.
In S5 based on the breeding method:
and (3) sowing the second generation seeds to obtain third generation rice plants which are albino leaf normal plants and albino leaf male sterile plants, eliminating the normal rice plants without albino leaves, and when the third generation plants are hybridized to the second generation plants to obtain third generation plants, the third generation plants cannot have normal plants without white leaf marks, but only have normal plants with white leaf mark characteristics and male sterile plants with white leaf marks, so that the cultivation proportion of male sterile plants can be effectively improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (6)
1. A breeding method of a sterile line of three-line hybrid rice with leaf marking property is characterized in that: the breeding method comprises the following steps:
s1: carrying out gene marking on the male parent rice plant of the primary sterile line;
s2: selecting a primary generation female rice plant, removing pistils, and hybridizing the primary generation female rice plant with a marked primary generation male rice plant;
s3: hybridizing the primary generation female rice plant with the primary generation male rice plant to obtain a secondary generation rice plant;
s4: selecting normal rice plants with whitened leaves with marking characteristics in the rice plants of the next generation and male plants of the sterile line with marking characteristics for hybridization;
s5: obtaining the third generation rice plants, and screening out the male sterile plants with the marking characteristics.
2. The breeding method of the sterile line of the three-line hybrid rice with the leaf marker trait of claim 1, which is characterized in that: in S1 based on the breeding method:
the recessive gene of the male sterile plant is marked with whitening characteristic by adopting a gene marking method, and the double recessive characteristic of the male sterile plant of the first generation is characterized by whitening of plant leaves.
3. The breeding method of the sterile line of the three-line hybrid rice with the leaf marker trait of claim 1, which is characterized in that: in S2 based on the breeding method:
the plant of the first generation mother selects a normal plant with normal dominant characteristics, and pistils are removed before the stage of stamens blooming, so as to ensure the success rate of pollination.
4. The breeding method of the sterile line of the three-line hybrid rice with the leaf marker trait of claim 1, which is characterized in that: in S3 based on the breeding method:
pollination is carried out on the first generation male parent and the first generation female parent rice plants in an artificial pollination mode, stamen pollen of the first generation female parent is contacted with stigma of pistil of the first generation sterile line, pollination and hybridization of the first generation male parent and the first generation female parent are realized, hybridized seeds are obtained, and the seeds are planted to obtain the second generation plants.
5. The breeding method of the sterile line of the three-line hybrid rice with the leaf marker trait of claim 1, which is characterized in that: in S4 based on the breeding method:
obtaining seeds after the hybridization of the primary female parent and the primary male parent, obtaining secondary rice plants, screening the secondary rice plants, selecting albino leaf normal plants with marked recessive genes and selecting albino leaf male sterile plants with marked recessive genes, and eliminating the albino leaf normal rice plants without marked recessive genes;
and (4) removing pistils from the albino normal plants of the second generation, and hybridizing with male sterile plants of the second generation to obtain seeds of the second generation.
6. The breeding method of the sterile line of the three-line hybrid rice with the leaf marker trait of claim 1, which is characterized in that: in S5 based on the breeding method:
and (3) sowing the second generation seeds to obtain third generation rice plants which are albino leaf normal plants and albino leaf male sterile plants, and eliminating normal rice plants without albino leaves.
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| CN202110816431.5A CN113475385A (en) | 2021-07-20 | 2021-07-20 | Breeding method of sterile line of three-line hybrid rice with leaf marking property |
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| CN202110816431.5A CN113475385A (en) | 2021-07-20 | 2021-07-20 | Breeding method of sterile line of three-line hybrid rice with leaf marking property |
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Citations (8)
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|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| AU2179099A (en) * | 1998-02-20 | 1999-09-06 | Syngenta Limited | Hybrid seed production |
| CN1283384A (en) * | 2000-08-18 | 2001-02-14 | 朱永峰 | Sterile line of hybridized rice with albino marker character on leaves and its new combination |
| US6737560B1 (en) * | 1988-02-03 | 2004-05-18 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
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2021
- 2021-07-20 CN CN202110816431.5A patent/CN113475385A/en active Pending
Patent Citations (8)
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| US6737560B1 (en) * | 1988-02-03 | 2004-05-18 | Pioneer Hi-Bred International, Inc. | Molecular methods of hybrid seed production |
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| AU2179099A (en) * | 1998-02-20 | 1999-09-06 | Syngenta Limited | Hybrid seed production |
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| CN101720665A (en) * | 2010-01-22 | 2010-06-09 | 四川省农业科学院生物技术核技术研究所 | Method for breeding male sterile line in ternary hybrid rice with orange red mark property |
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| CN104054570A (en) * | 2014-06-26 | 2014-09-24 | 浙江大学 | Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs |
| CN104160950A (en) * | 2014-08-14 | 2014-11-26 | 淮南市开元生物技术研究中心 | Method for cultivating hybrid rice species by utilizing rice albino seedling traits |
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