CN106636354A - Method for increasing disease-resistant breeding efficiency of hybrid rice - Google Patents

Method for increasing disease-resistant breeding efficiency of hybrid rice Download PDF

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CN106636354A
CN106636354A CN201611009611.8A CN201611009611A CN106636354A CN 106636354 A CN106636354 A CN 106636354A CN 201611009611 A CN201611009611 A CN 201611009611A CN 106636354 A CN106636354 A CN 106636354A
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resistance gene
sterile line
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官华忠
毛大梅
陈志伟
潘润森
吴为人
兰涛
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a method for increasing the disease-resistant breeding efficiency of hybrid rice, and belongs to the field of disease-resistant breeding of the hybrid rice. The method comprises the following steps: creating germplasm containing a bacterial blight resistance gene and rice blast resistance gene chromosome segment or a salt resistance gene and rice blast resistance gene chromosome segment by utilizing a molecular marker-assisted selection technology, and respectively hybridizing with a three-line maintainer line, a two-line sterile line or a restoring line; screening resistant individual plants for each segregative generation in a seedling stage by utilizing bacterial blight germs until screening out a new maintainer line B-resistant plant, a two-line sterile line S-resistant plant or a new restoring line R-resistant plant which obtains genetic stability and accords with breeding purposes; matching by using a sterile line containing the bacterial blight resistance gene and rice blast resistance gene chromosome segment and a restoring line containing the salt resistance gene and rice blast resistance gene chromosome segment, thus obtaining a hybrid variety containing the bacterial blight resistance gene, the rice blast resistance gene and the salt resistance gene. The method provided by the invention is simple and quick, and the disease-resistant breeding efficiency of the hybrid rice is increased.

Description

A kind of method for improving hybrid rice breeding for disease resistance efficiency
Technical field
Present invention relates particularly to a kind of method for improving the disease-resistant fertility efficiency of hybrid rice, belongs to breeding for disease resistance field.
Background technology
It is a kind of conventional means that rice molecular marker-assisted breeding is current paddy disease-resistant breeding, but domestic at present logical In crossing the rice varieties of authorization above the provincial level, the kind being bred as by the method for molecular mark is also little.Cause Stating the main cause of present situation has：（1）Many breeding units domestic at present are particularly basic unit's breeding units does not have condition or technology to adopt Breeding for disease resistance is carried out with molecular mark technology；（2）The anti-source for carrying multiple disease-resistant genes simultaneously at present is few, disease-resistant Breeding it is inefficient.In addition, blast resistance identification is affected by environment big, have that qualification result is unstable and inefficiency etc. Problem.Therefore, the problem that paddy disease-resistant breeding efficiency is rice breeding worker's general concern how is improved.
As Sequencing of Rice Genome is completed, increasing resistant gene is such as reflected at present by finely positioning and clone Fixed 84 main effect blast resistant genes（Wherein 24 genes are cloned）, identify 38 Bacterial blight resistance genes（Wherein 7 bases Because being cloned）With cloned 4 genes relevant with salt tolerant.Found according to these results, positioned at the anti-of No. 11 chromosomes of paddy rice Bacterial leaf-blight geneXa23With blast resistant genePb-1There is linkage relationship, physical distance is 595.536kb；Positioned at paddy rice 6 The blast resistant gene of number chromosomePi-tq1And resistant gene of saltsstAlso there is linkage relationship, physical distance is about 2000.00kb left and right.According to the principle of linkage inheritance, it is possible to useXa23Gene or resistant gene of saltsstIt is attached thereto to screen The rice blast resistance gene of lockPb-1OrPi-tq1.Therefore, rice blast resistance is screened using leaf spot bacteria and NaCL solution Gene is feasible.
The content of the invention
It is an object of the invention to provide a kind of method for improving hybrid rice breeding for disease resistance efficiency, former using linkage inheritance Reason and heterosis utilization breeding method, efficiently screen and prepare " have blast resistant gene, Bacterial blight resistance gene and The hybrid rice seed of resistant gene of salt ".
Technical scheme is as follows：
（1）Contain " Bacterial blight resistance gene+blast resistant gene " or " salt tolerant using Molecular Marker Assisted Selection Technology initiative The germplasm of gene+blast resistant gene " chromosome segment；Wherein germplasm first includes the blast resisting positioned at No. 11 chromosome of paddy rice GenePb-1And Bacterial blight resistance geneXa23；Germplasm second includes the blast resistant gene positioned at No. 6 chromosome of paddy ricePi-tq1 And resistant gene of saltsst；
（2）Utilize（1）The germplasm first of middle acquisition is the hybridization of maintainer, two-line sterile line or restorer with three, obtains hybrid seed； Because the rice blast resistance gene included in germplasm first and bacterial leaf spot resistance gene are chain, so in the seedling of segregating generation Phase, using the specialization identification bacterial strain of bacterial leaf spot disease-resistant gene, to segregating population inoculated identification is carried out, and eliminates the seedling of sense hoja blanca, is resisted Hoja blanca, while carry blast resistant gene seedling be transplanted to land for growing field crops, later stage breeding method routinely is selected；It is so many In generation, repeats, and until selecting, to obtain inheritance stability and meet breeding objective three be the anti-B1 of maintainer, the anti-S1 of two-line sterile line or extensive It is again anti-R1, these germplasm all carry bacterial blight-resisting and rice blast ospc gene；It is that the anti-B1 of maintainer carries out 1 time with sterile line with three Hybridization, six backcrossings are cultivated and obtain the anti-A1 of sterile line, and the sterile line is containing bacterial blight-resisting and rice blast ospc gene；
（3）With（1）The germplasm second of middle acquisition is the hybridization of maintainer, two-line sterile line or restorer with three, obtains hybrid seed；Cause Rice blast resistance gene and resistant gene of salt included in germplasm second is chain, so in segregating generation seedling disk after planting, profit Poured with the NaCl of 150mmol/L, eliminate the seedling of not salt tolerant, salt tolerant carries the seedling of blast resistant gene and is transplanted to land for growing field crops simultaneously, Later stage breeding method routinely is selected；In so many generation, repeats, and obtains inheritance stability and meets breeding objective until selecting Three is the anti-B2 of maintainer, the anti-S2 of two-line sterile line or the anti-R2 of restorer, and these resources all carry resistant gene of salt and blast resisting base Cause；According to sterile line breeding process, with the anti-B2 of maintainer and two-line sterile line through 1 hybridization, 6 backcrossings, sterile line is cultivated " anti-A2 ", the sterile line is containing resistant gene of salt and rice blast ospc gene；
（4）With（2）In the anti-A1 of sterile line or the anti-S1 of two-line sterile line with（3）In restorer anti-R2 hybridization production hybrid f1Generation Seed " miscellaneous f1", or use（3）In the anti-A2 of sterile line or the anti-S2 of two-line sterile line with（2）In restorer anti-R1 hybridization productions Hybrid f1For seed " miscellaneous f1", the cenospecies for being obtained contains Bacterial blight resistance gene, blast resistant gene and resistant gene of salt；
（5）Hybrid f1For seed " miscellaneous f1" i.e. as production kind.
Its concrete grammar is：
（1）Contain " Bacterial blight resistance gene+blast resistant gene " or " salt tolerant using Molecular Marker Assisted Selection Technology initiative The germplasm of gene+blast resistant gene " chromosome segment；Such as by Modan（Carry blast resistingPb-1）And CBB23（Carry anti- Bacterial leaf-blight geneXa23）Hybridization, offspring withPb-1Gene andXa23The molecular marker screening of gene linkage is contained “Xa23+ Pb-1" chromosome segment germplasm first.By salt-tolerant mutant（Carry resistant gene of saltsst, obtained by R401 radiation）With It is special blue or green（Carry blast resistingPi-tq1）Hybridization, offspring contained with same method "sst+ Pi-tq1" chromosome segment Germplasm second.
（2）Utilize（1）The new germ plasm first of middle acquisition and three is maintainer（Such as suitable perfume 1B）, two-line sterile line（Such as grand section 638S）Or restorer（As good fortune extensive 673 or China account for）Hybridization, obtains hybrid seed.Because of the rice blast resistance base included in germplasm A Cause and bacterial leaf spot resistance gene are chain, so in segregating generation（From F2Or BC1F1）, seedling stage using bacterial leaf spot it is disease-resistant The specialization identification bacterial strain of gene, to segregating population inoculated identification is carried out, and eliminates the seedling of sense hoja blanca, the seedling of anti-hoja blanca（Simultaneously Carry blast resistant gene）Land for growing field crops is transplanted to, later stage breeding method routinely is selected.In so many generation, repeats, Zhi Daoxuan It is the anti-B1 of maintainer to go out inheritance stability and meet breeding objective three（The new holding for obtaining such as is improved using this method by suitable fragrant 1B System）, the anti-S1 of two-line sterile line（The new two-line sterile line for obtaining such as is improved using this method by grand section 638S）Or restorer is anti- R1（Such as accounted for by China and the new restorer line for obtaining is improved using this method）, these new resources all carry bacterial blight-resisting and rice blast base Cause.With the anti-B1 of maintainer and sterile line（Such as suitable perfume 1A）Through 1 hybridization, BC is returned to 6 times6F1, cultivate sterile line " anti-A1 " （Concrete Breeding Process is with reference to table 1）, containing bacterial blight-resisting and rice blast ospc gene；
（3）With（1）The new germ plasm second of middle acquisition and three is maintainer（Such as suitable perfume 1B）, two-line sterile line（Such as grand section 638S）Or it is extensive Multiple system（As good fortune extensive 673 or China account for）Hybridization, obtains hybrid seed.Because of the rice blast resistance gene included in " resisting more " germplasm and Resistant gene of salt is chain, so in segregating generation（From F2Or BC1F1）, using 150mmol/L's in seedling disk after planting NaCL is poured, and eliminates the seedling of not salt tolerant, the seedling of salt tolerant（Blast resistant gene is carried simultaneously）It is transplanted to land for growing field crops, the later stage is routinely Breeding method is selected.In so many generation, repeats, until select obtain inheritance stability and meet the anti-B2 of the new maintainer of breeding objective, The anti-S2 of the two-line sterile line or anti-R2 of new restorer line, these new resources all carry resistant gene of salt and blast resistant gene.Use maintainer Anti- B2 and sterile line（Such as suitable perfume 1A）Through 1 hybridization, BC is returned to 6 times6F1, new sterile line " anti-A2 " is cultivated, containing resistant gene of salt With rice blast ospc gene（Concrete Breeding Process is with reference to following table）；
The Breeding Process of the anti-A of table 1
（4）With（2）In anti-A1 or S1 with（3）In anti-R2 hybridization production hybrid f1For seed " miscellaneous f1", or use（3）In Anti- A2 or S2 with（2）In anti-R1 hybridization production hybrid f1For seed " miscellaneous f1", the cenospecies for being obtained contains bacterial blight-resisting Gene, blast resistant gene and resistant gene of salt；
（5）Hybrid f1For seed " miscellaneous f1" can be used as production kind.
The method for screening rice blast resistance gene using leaf spot bacteria, will be easy to the bacterial leaf spot resistance identified in form Gene alternatively marks to screen blast resistant gene chain therewith.
The method for screening rice blast resistance gene using the NaCL solution of 150mmol/L, will be easy to the resistance to of identification in form Salt gene alternatively marks to screen blast resistant gene chain therewith.
Beneficial effects of the present invention：
Effect of the invention is that can alternatively mark to screen the anti-of more difficult identification using the resistant gene for being easier to identify Property gene, such as connects bacterium and certain density NaCL solution to screen rice blast resistance gene using bacterial leaf-blight.Simultaneously by miscellaneous Kind use of advantage together, is cultivated the resistant gene rapid polymerization contained by Parent to contain Bacterial blight resistance gene, anti-rice The cenospecies of seasonal febrile diseases gene and resistant gene of salt.
It is an advantage of the invention that the basic unit's breeding units for unconditionally carrying out molecular marking technique are anti-using what is createed Property germplasm is improving breeding for disease resistance efficiency.
Specific embodiment：
（Note these resistant varieties can find in national paddy rice data center）
Embodiment 1：
1) by Modan（Carry blast resistingPb-1）And CBB23（Carry Bacterial blight resistance geneXa23）Hybridization, offspring withPb-1Gene andXa23The molecular marker screening of gene linkage contained "Xa23+ Pb-1" chromosome segment germplasm first.Will Salt-tolerant mutant（Carry resistant gene of saltsst, obtained by R401 radiation）It is blue or green with spy（Carry blast resistingPi-tq1）Hybridization, offspring Contained with same method "sst+ Pi-tq1" chromosome segment germplasm second；
2) will suitable perfume 1B and 1）Gained germplasm first（Xa23+ Pb-1）Hybridized, obtained F1Seed；
3) 2 are planted）The F of gained1.During heading, with suitable perfume 1B as female parent, F1For male parent, hybridized, obtained BC1F1Seed；
4) 3 are planted）The BC of gained1F1Seed.Seedling stage, 120 plants of individual plant for screening bacterial blight-resisting by leaf spot bacteria P6 is taken out 10 plants of other proterties individual plant more consistent with suitable fragrant 1B and suitable fragrant 1B are selected after fringe（Recurrent parent）, obtain BC2F1Seed；
5) 4 are planted）The BC of gained2F1Seed.Seedling stage filters out bacterial blight-resisting individual plant 120 also with leaf spot bacteria P6 Strain, selects 10 plants of other proterties individual plant more consistent with suitable fragrant 1B and suitable fragrant 1B after heading（Recurrent parent）, obtain BC3F1Seed；
6) 5 are planted）The BC of gained3F1Seed.Seedling stage filters out bacterial blight-resisting individual plant 120 also with leaf spot bacteria P6 Strain, selects 10 plants of the individual plant that other proterties are more consistent with suitable fragrant 1B, selfing to obtain BC after heading3F2Seed；
7) 6 are planted）The BC of gained3F2Seed.Seedling stage filters out bacterial blight-resisting individual plant 120 also with leaf spot bacteria P6 Strain, selects 20 plants of the individual plant that other proterties are more consistent with suitable fragrant 1B, selfing to obtain BC after heading3F3Seed；
8) 7 are planted by cell）The BC of gained3F3Seed.Seedling stage filters out also with leaf spot bacteria P6 and shows as resisting white leaf Rot and the unseparated area of cell 7 of resistance, select that other proterties are more consistent with suitable fragrant 1B, cell neat and consistent strain after heading System, it is final be bred as containing "Xa23+ Pb-1" chromosome segment and other proterties maintainer consistent with suitable fragrant 1B be " preferably fragrant anti- 2B”；Hybridized with suitable fragrant 1A using the maintainer simultaneously, obtain F1Seed；
9) 8 are planted）The F of gained1Seed.After heading, F1In generation, continues and " preferably fragrant anti-2B "（Male parent）Backcrossing, obtains BC1F1Seed；
10) 9 are planted）The BC of gained1F1Seed.Seedling stage carries out connecing the dientification of bacteria also with leaf spot bacteria P6 to sterile line, sieve Bacterial blight-resisting sterile line is selected, the individual plant that other proterties are more consistent with " preferably fragrant anti-2B " is selected after heading, with " preferably fragrant anti-2B " （Male parent）Backcrossing, obtains BC1F1Seed；
11) 10 are planted）The BC of gained2F1Seed.Seedling stage carries out connecing the dientification of bacteria also with leaf spot bacteria P6 to sterile line, sieve Bacterial blight-resisting sterile line is selected, the individual plant that other proterties are more consistent with " preferably fragrant anti-2B " is selected after heading, with " preferably fragrant anti-2B " （Male parent）Backcrossing, obtains BC3F1Seed；
12) 11 are planted）The BC of gained3F1Seed.Seedling stage carries out connecing the dientification of bacteria also with leaf spot bacteria P6 to sterile line, sieve Bacterial blight-resisting sterile line is selected, other proterties are selected after heading with " preferably fragrant anti-2B " more consistent and cell neat and consistent strain System, it is final be bred as containing "Xa23+ Pb-1" chromosome segment and other proterties maintainer consistent with suitable fragrant 1B be " preferably fragrant anti- 2A”；
13) with good fortune extensive 673 as maternal, with 1）Gained germplasm second（sst+ Pi-tq1）Hybridization, obtains f1Seed；
14) 13 are planted）The f of gained1Seed, selfing obtains f2Seed；
15) 14 are sowed in seedling disk）The f of gained2Seed.After planting, using 150mmol/L NaCL solution pour, eliminate intolerant to The seedling of salt, obtains 1000 plants of the seedling of salt tolerant（Blast resistant gene is carried simultaneously）Be transplanted to land for growing field crops, the later stage breeding side routinely Method carries out leaf morphology selection, obtains 100 plants of individual plants（f3Seed）；
16) 15 are sowed in seedling disk by cell）The v of gained3Seed.Seedling stage screens salt tolerant and unseparated strain also with salting liquid It is 30th area, later-stage utilization pedigree method is selected agronomy, yield traits and made its selfing, obtains F4Seed；
17) 16 are sowed in seedling disk by cell）The f of gained4Seed.Seedling stage screens the strain of salt tolerant, later stage also with salting liquid Its selfing is selected and made to agronomy, yield traits, restoring force, recovery spectrum and coordinate force using pedigree method, f is obtained5Kind Son；
18) 16 are sowed in seedling disk by cell）The f of gained4Seed.Seedling stage screens the strain of salt tolerant, later stage also with salting liquid Using pedigree method to agronomy, yield traits, restoring force, recover spectrum and coordinate force is selected, it is final be bred as containing "sst+ Pi- tq1" salt tolerant of chromosome segment resists extensive No. 1；
19) " preferably fragrant anti-2A "（Genotype isXa23Xa23Pb-1Pb-1SSTSSTpi-tq1pi-tq1）/ " salt tolerant resists extensive No. 1 " （Genotype issstsstPi-tq1Pi-tq1xa23xa23pb-1pb-1）→ production is used kind of " preferably fragrant anti-excellent No. 1 "（Genotype isXa23xa23Pb-1pb-1SSTsst Pi-tq1pi-tq1）.
Embodiment 2：
1) the suitable fragrant anti-2B that grand section 638S is obtained with example one（Xa23+ Pb-1）Hybridization, obtains F1 Seed；
2) F is planted1, the later stage harvest, obtain F2Seed；
3) 2 are planted）Gained F2Seed.1000 plants of the individual plant of bacterial blight-resisting is screened with leaf spot bacteria P6 in seedling stage, the later stage presses Conventional breeding methods are selected fertility and economical character, obtain sterile 100 plants of individual plant so as to which selfing obtains F3Seed；
4) 3 are planted by cell）Gained F3Seed.Bacterial blight-resisting is screened in seedling stage with leaf spot bacteria P6 and non-resistance is not separated The area of strain 35, later-stage utilization pedigree method carries out selecting 30 strains to agronomy, yield traits, fertility, and selfing obtains F4Seed；
5) 4 are planted）Gained F4Seed.The area of strain 30 of bacterial blight-resisting, later-stage utilization are screened with leaf spot bacteria P6 in seedling stage Pedigree method carries out selecting 25 strains to agronomy, yield traits, fertility and coordinate force, and selfing obtains F5Seed；
6) 5 are planted）Gained F5Seed.The area of strain 25 of bacterial blight-resisting, later-stage utilization are screened with leaf spot bacteria P6 in seedling stage Pedigree method is selected agronomy, yield traits, fertility and coordinate force, it is final be bred as containing "Xa23+ Pb-1" chromosome segment Grand anti-S；
7) salt tolerant that Hua Zhanyu examples one are obtained resists extensive No. 1（sst+ Pi-tq1）Hybridization, obtains f1Seed；
8) 7 are planted）The f of gained1Seed, selfing obtains f2Seed；
9) 8 are sowed in seedling disk）The f of gained2Seed.After planting, poured using the NaCL solution of 150mmol/L, eliminate not salt tolerant Seedling, obtain salt tolerant 1000 plants of seedling（Blast resistant gene is carried simultaneously）It is transplanted to land for growing field crops, later stage breeding method routinely Leaf morphology selection is carried out, 100 plants of individual plants are obtained（f3Seed）；
10) 9 are sowed in seedling disk by cell）The f of gained3Seed.Seedling stage screens salt tolerant and unseparated strain also with salting liquid It is 30th area, later-stage utilization pedigree method is selected agronomy, yield traits and made its selfing, obtains F4Seed；
11) 10 are sowed in seedling disk by cell）The f of gained4Seed.Seedling stage screens the strain of salt tolerant, later stage also with salting liquid Its selfing is selected and made to agronomy, yield traits, restoring force, recovery spectrum and coordinate force using pedigree method, f is obtained5Kind Son；
12) 11 are sowed in seedling disk by cell）The f of gained5Seed.Seedling stage screens the strain of salt tolerant, later stage also with salting liquid Using pedigree method to agronomy, yield traits, restoring force, recover spectrum and coordinate force is selected, it is final be bred as containing "sst+ Pi- tq1" salt tolerant of chromosome segment resists extensive No. 2；
13) " grand anti-S "（Genotype isXa23Xa23Pb-1Pb-1SSTSSTpi-tq1pi-tq1）/ " salt tolerant resists extensive No. 2 "（Base Because type isxa23xa23pb-1pb-1sstsstPi-tq1Pi-tq1）→ production is with kind of " grand anti-two excellent No. 2 "（Genotype isXa23xa23Pb-1pb-1SSTsst Pi-tq1pi-tq1）.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with Modification, should all belong to the covering scope of the present invention.

Claims (1)

1. it is a kind of improve hybrid rice breeding for disease resistance efficiency method, it is characterised in that：The selection is comprised the following steps：
（1）Contain " Bacterial blight resistance gene+blast resistant gene " or " salt tolerant using Molecular Marker Assisted Selection Technology initiative The germplasm of gene+blast resistant gene " chromosome segment；Wherein germplasm first includes the blast resisting positioned at No. 11 chromosome of paddy rice GenePb-1And Bacterial blight resistance geneXa23；Germplasm second includes the blast resistant gene positioned at No. 6 chromosome of paddy ricePi-tq1 And resistant gene of saltsst；
（2）Utilize（1）The germplasm first of middle acquisition is the hybridization of maintainer, two-line sterile line or restorer with three, obtains hybrid seed； Because the rice blast resistance gene included in germplasm first and bacterial leaf spot resistance gene are chain, so in the seedling of segregating generation Phase, using the specialization identification bacterial strain of bacterial leaf spot disease-resistant gene, to segregating population inoculated identification is carried out, and eliminates the seedling of sense hoja blanca, is resisted Hoja blanca, while carry blast resistant gene seedling be transplanted to land for growing field crops, later stage breeding method routinely is selected；It is so many In generation, repeats, and until selecting, to obtain inheritance stability and meet breeding objective three be the anti-B1 of maintainer, the anti-S1 of two-line sterile line or extensive It is again anti-R1, these germplasm all carry bacterial blight-resisting and rice blast ospc gene；It is that the anti-B1 of maintainer carries out 1 time with sterile line with three Hybridization, six backcrossings are cultivated and obtain the anti-A1 of sterile line, and the sterile line is containing bacterial blight-resisting and rice blast ospc gene；
（3）With（1）The germplasm second of middle acquisition is the hybridization of maintainer, two-line sterile line or restorer with three, obtains hybrid seed；Cause Rice blast resistance gene and resistant gene of salt included in germplasm second is chain, so in segregating generation seedling disk after planting, profit Poured with the NaCl of 150mmol/L, eliminate the seedling of not salt tolerant, salt tolerant carries the seedling of blast resistant gene and is transplanted to land for growing field crops simultaneously, Later stage breeding method routinely is selected；In so many generation, repeats, and obtains inheritance stability and meets breeding objective until selecting Three is the anti-B2 of maintainer, the anti-S2 of two-line sterile line or the anti-R2 of restorer, and these resources all carry resistant gene of salt and blast resisting base Cause；According to sterile line breeding process, with the anti-B2 of maintainer and two-line sterile line through 1 hybridization, 6 backcrossings, sterile line is cultivated " anti-A2 ", the sterile line is containing resistant gene of salt and rice blast ospc gene；
（4）With（2）In the anti-A1 of sterile line or the anti-S1 of two-line sterile line with（3）In restorer anti-R2 hybridization production hybrid f1Generation Seed " miscellaneous f1", or use（3）In the anti-A2 of sterile line or the anti-S2 of two-line sterile line with（2）In restorer anti-R1 hybridization productions Hybrid f1For seed " miscellaneous f1", the cenospecies for being obtained contains Bacterial blight resistance gene, blast resistant gene and resistant gene of salt；
（5）Hybrid f1For seed " miscellaneous f1" i.e. as production kind.
CN201611009611.8A 2016-11-17 2016-11-17 Method for increasing disease-resistant breeding efficiency of hybrid rice Pending CN106636354A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962185A (en) * 2017-05-26 2017-07-21 宁波市农业科学研究院 It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
CN108739357A (en) * 2018-06-02 2018-11-06 福建农林大学 A kind of selection of the drought-enduring anti-fall double-linear hybrid rice combination of naked body salt tolerant
CN108739356A (en) * 2018-06-02 2018-11-06 福建农林大学 A kind of method for creating of the high-quality odor type naked body rice germplasm of high setting percentage salt tolerant
CN108901820A (en) * 2018-06-02 2018-11-30 福建农林大学 A kind of salt tolerant naked body Three-line rice sterile line breeding method
CN109042304A (en) * 2018-09-04 2018-12-21 江苏里下河地区农业科学研究所 The selection of blast resisting good quality and high output two-line hybrid rice combination

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Application publication date: 20170510