CN106962185A - It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance - Google Patents
It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance Download PDFInfo
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- CN106962185A CN106962185A CN201710395372.2A CN201710395372A CN106962185A CN 106962185 A CN106962185 A CN 106962185A CN 201710395372 A CN201710395372 A CN 201710395372A CN 106962185 A CN106962185 A CN 106962185A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of while the breeding method of raising conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance, belongs to breeding technical field.The breeding method of the present invention is blast resistant gene using indica type blast resisting two-line sterile line and two be the donor parents of sterile gene, to produce the conventional japonica rice of upper large-area applications as receptor parent, by once hybridizing, it is returned for three times, in combination with methods such as molecular marker assisted selections, in BC3F2Do final election from generation to generation, fertile individual plant can be used for cultivating blast resisting conventional japonica rice new varieties, sterile individual plant can be used for cultivating blast resisting round-grained rice type two-line sterile line, the breeding method obtains two kinds of breeding achievement materials by a kind of breeding methods, it is clear and definite with breeding objective, breeding method is simple, and breeding results outstanding feature greatly reduces the input of manpower and financial resources in breeding.
Description
Technical field
It is more particularly to a kind of to improve conventional japonica rice and round-grained rice type two-line sterile line rice simultaneously the invention belongs to breeding technical field
The breeding method of seasonal febrile diseases resistance.
Background technology
With the raising and the progress of agriculture production technology of living standards of the people, green, safe and healthy fine quality rice is needed
Asking increasingly increases, and this proposes higher requirement to breeder.Crossbreeding is the main of current conventional japonica rice breeding of new variety
Approach, but its Juvenile stage is only confined between conventional japonica rice, genetic background is narrow, no to make full use of current water
The germ plasm resource of rice, and breeding objective can only be confined in seed selection conventional japonica rice new varieties.The present invention is indica type using blast resisting
Two-line sterile line is blast resisting and two be the donor parents of sterile gene, using high-quality conventional japonica rice as receptor parent, by miscellaneous
Hand over, backcrossing and molecular marker assisted selection, can simultaneously seed selection blast resisting conventional japonica rice new varieties and round-grained rice type two-line sterile line,
With breeding objective clearly, breeding method is simple, and breeding results outstanding feature greatly reduces manpower and financial resources in breeding
Input.
The content of the invention
It is an object of the invention to overcome shortcoming and deficiency present in above-mentioned prior art there is provided a kind of while improving normal
Advise the breeding method of japonica rice and round-grained rice type two-line sterile line rice blast resistance.This method is using indica type blast resisting two-line sterile line
Blast resistant gene and two be sterile gene donor parents, to produce the conventional japonica rices of upper large-area applications as receptor parent,
By once hybridizing, it is returned for three times, in combination with methods such as molecular marker assisted selections, in BC3F2Do final election, fertile list from generation to generation
Strain can be used for cultivating blast resisting conventional japonica rice new varieties, and sterile individual plant can be used for cultivating blast resisting round-grained rice type two-line sterile line,
The breeding method obtains two kinds of breeding achievement materials by a kind of breeding methods, with breeding objective clearly, and breeding method is simple,
Breeding results outstanding feature, greatly reduces the input of manpower and financial resources in breeding.
The purpose of the present invention is achieved through the following technical solutions：It is a kind of to improve conventional japonica rice and round-grained rice type two-line sterile line simultaneously
The breeding method of rice blast resistance, comprises the following steps：
(1) done and hybridized with indica type blast resisting two-line sterile line and conventional japonica rice, plantation hybrid F1Generation, molecular labeling auxiliary
The positive individual plant of selection is returned with conventional japonica rice, obtains BC1F1For seed；
(2) BC is planted1F1Generation, molecular marker assisted selection positive individual plant is returned with conventional japonica rice, is harvested simultaneously
BC1F2For seed；
(3) BC is planted2F1Generation, molecular marker assisted selection positive individual plant is returned with conventional japonica rice, harvests BC3F1Generation
Seed；
(4) BC is planted3F1Generation, molecular marker assisted selection positive individual plant harvest BC3F2For seed；
(5) BC is planted by strain3F2Generation selection colony, carries out molecular marker assisted selection, in positive homozygosis individual plant, choosing
Select the good individual plant harvest BC of setting percentage height, big panicle many grains per panicle, yielding ability3F3For seed, simultaneous selection thoroughly infertility, flowering habit compared with
Good, the individual plant that plant is not higher than 85cm cuts root and stem of certain plants regeneration, and BC is harvested under short day cryogenic conditions3F3For seed.
For fertile individual plant, the experiments, final choice such as blast resistance identification, Yield test and rice quality analysis are carried out
The strain that rice blast resistance is good, yield is high, rice quality is excellent symptom of a trend strain the most is used for experiments at different levels；For sterile individual plant, enter
Row combining ability test, in combination with blast resistance identification, Fertility identification and rice quality analysis etc., is selected in long-day conditions
Under fertility critical-temperature can be reduced to 21 DEG C or so, the fertility critical-temperature under the conditions of short-day brings up to 25 DEG C of left sides
The right side, while the paddy rice japonica rice two-line sterile line that rice blast resistance is good, general combining ability is high, rice quality is excellent is identified.
Indica type blast resisting two-line sterile line is preferably magnificent 1015S described in step (1).
Conventional japonica rice described in step (1) is preferably peaceful 84 or No. 2 B of river in Zhejiang Province round-grained rice (B of Ningbo 2).
Molecular marker assisted selection described in step (1)~(5) is takes Pi2 upstream region of gene Indel marks to select, Pi2
One or both of downstream of gene SSR marker selection；
The used primer information of the Pi2 upstream region of gene Indel marks selection is as follows：
Pi2 upstream region of gene marks forward primer：GGTCGTGTTGCATCGGTAAG；
Pi2 upstream region of gene marks reverse primer：TTCCCCCATCGTGCAAATTG；
The Pi2 downstream of gene SSR marker selects used primer information as follows：
Pi2 downstream of gene marks forward primer：TCCAAGACTGAACAGCGAGA；
Pi2 downstream of gene marks reverse primer：TGCGAATCAAATCACTGCAC.
Step harvests BC described in (2)1F2After seed, quality sorting is preferably carried out, the quality sorting is to enter indoors
Row rice quality is analyzed, and is eliminated the poor individual plant of exterior quality and its is done backcrossing combination.
Under the conditions of short day cryogenic conditions described in step (5) are preferably 11h~12h sunshines, 18 DEG C~22 DEG C.
The invention discloses a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance.
This method is blast resistant gene using indica type blast resisting two-line sterile line and two be the donor parents of sterile gene, to produce
The conventional japonica rice of upper large-area applications is receptor parent, by once hybridizing, is returned for three times, aids in selecting in combination with molecular labeling
The method such as select, in BC3F2Final election is done from generation to generation, and fertile individual plant can be used for cultivating blast resisting conventional japonica rice new varieties, and sterile individual plant can
For cultivating blast resisting round-grained rice type two-line sterile line, the breeding method obtains two kinds of breeding achievement materials by a kind of breeding methods
Material, breeding method simple with breeding objective clearly, and breeding results outstanding feature greatly reduces manpower and wealth in breeding
The input of power.
The present invention has the following advantages and effect relative to prior art：
1. the seed selection of conventional japonica rice and round-grained rice type two-line sterile line is attached in a breeding methods, breeding is greatly accelerated
Process, reduces the input of manpower and materials, improves operating efficiency.
2. the seed selection of conventional japonica rice and round-grained rice type two-line sterile line is combined with the improvement of its rice blast resistance, increase disease-resistant
Conventional japonica rice and the probability of round-grained rice type two-line sterile line selection.
3. whole breeding process is in BC3F2Just start to select sterile individual plant after generation, reduce the number of early stage rice regeneration strain
Amount, while improving the probability for choosing excellent sterile system.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
The invention provides a kind of while improve the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance,
Comprise the following steps：
(1) done and hybridized with indica type blast resisting two-line sterile line China 1015S and conventional japonica rice peaceful 84, plantation hybrid F1Generation,
Molecular marker assisted selection positive individual plant is returned with peaceful 84, obtains BC1F1For seed；
(2) BC is planted1F1Generation, molecular marker assisted selection positive individual plant is returned with peaceful 84, while harvesting BC1F2Generation
Seed；
(3) BC is planted2F1Generation, molecular marker assisted selection positive individual plant is returned with peaceful 84, harvests BC3F1For seed；
(4) BC is planted3F1Generation, molecular marker assisted selection positive individual plant harvest BC3F2For seed；
(5) BC is planted by strain3F2Generation selection colony, carries out molecular marker assisted selection, in positive homozygosis individual plant, choosing
Select the good individual plant harvest BC of setting percentage height, big panicle many grains per panicle, yielding ability3F3For seed, simultaneous selection thoroughly infertility, flowering habit compared with
Good, the individual plant that plant is not higher than 85cm cuts root and stem of certain plants regeneration, and BC is harvested under short day cryogenic conditions3F3For seed.
Molecular marker assisted selection described in step (1)~(5) selects to take Pi2 upstream region of gene Indel to mark；
The used primer information of the Pi2 upstream region of gene Indel marks selection is as follows：
Pi2 upstream region of gene marks forward primer：GGTCGTGTTGCATCGGTAAG；
Pi2 upstream region of gene marks reverse primer：TTCCCCCATCGTGCAAATTG.
Step harvests BC described in (2)1F2After seed, quality sorting is preferably carried out, the quality sorting is to enter indoors
Row rice quality is analyzed, and is eliminated the poor individual plant of exterior quality and its is done backcrossing combination.
Under the conditions of short day cryogenic conditions described in step (5) are preferably 11h~12h sunshines, 18 DEG C~22 DEG C.
For fertile individual plant, the experiments, final choice such as blast resistance identification, Yield test and rice quality analysis are carried out
The strain that rice blast resistance is good, yield is high, rice quality is excellent symptom of a trend strain the most is used for experiments at different levels；For sterile individual plant, enter
Row combining ability test, in combination with blast resistance identification, Fertility identification and rice quality analysis etc., is selected in long-day conditions
Under fertility critical-temperature can be reduced to 21 DEG C or so, the fertility critical-temperature under the conditions of short-day brings up to 25 DEG C of left sides
The right side, while the paddy rice japonica rice two-line sterile line that rice blast resistance is good, general combining ability is high, rice quality is excellent is identified.
Embodiment 2
The invention provides a kind of while improve the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance,
Comprise the following steps：
(1) done and hybridized with indica type blast resisting two-line sterile line China 1015S and No. 2 B of conventional japonica rice river in Zhejiang Province round-grained rice (B of Ningbo 2),
Plant hybrid F1Generation, molecular marker assisted selection positive individual plant is returned with No. 2 B of river in Zhejiang Province round-grained rice (B of Ningbo 2), obtains BC1F1Generation
Seed；
(2) BC is planted1F1Generation, molecular marker assisted selection positive individual plant is returned with No. 2 B of river in Zhejiang Province round-grained rice (B of Ningbo 2), together
When harvest BC1F2For seed；
(3) BC is planted2F1Generation, molecular marker assisted selection positive individual plant is returned with No. 2 B of river in Zhejiang Province round-grained rice (B of Ningbo 2), is received
Obtain BC3F1For seed；
(4) BC is planted3F1Generation, molecular marker assisted selection positive individual plant harvest BC3F2For seed；
(5) BC is planted by strain3F2Generation selection colony, carries out molecular marker assisted selection, in positive homozygosis individual plant, choosing
Select the good individual plant harvest BC of setting percentage height, big panicle many grains per panicle, yielding ability3F3For seed, simultaneous selection thoroughly infertility, flowering habit compared with
Good, the individual plant that plant is not higher than 85cm cuts root and stem of certain plants regeneration, and BC is harvested under short day cryogenic conditions3F3For seed.
Molecular marker assisted selection described in step (1)~(5) selects to take Pi2 upstream region of gene Indel to mark simultaneously
With the selection of Pi2 downstream of gene SSR marker；
The used primer information of the Pi2 upstream region of gene Indel marks selection is as follows：
Pi2 upstream region of gene marks forward primer：GGTCGTGTTGCATCGGTAAG；
Pi2 upstream region of gene marks reverse primer：TTCCCCCATCGTGCAAATTG；
The Pi2 downstream of gene SSR marker selects used primer information as follows：
Pi2 downstream of gene marks forward primer：TCCAAGACTGAACAGCGAGA；
Pi2 downstream of gene marks reverse primer：TGCGAATCAAATCACTGCAC.
Step harvests BC described in (2)1F2After seed, quality sorting is preferably carried out, the quality sorting is to enter indoors
Row rice quality is analyzed, and is eliminated the poor individual plant of exterior quality and its is done backcrossing combination.
Under the conditions of short day cryogenic conditions described in step (5) are preferably 11h~12h sunshines, 18 DEG C~22 DEG C.
For fertile individual plant, the experiments, final choice such as blast resistance identification, Yield test and rice quality analysis are carried out
The strain that rice blast resistance is good, yield is high, rice quality is excellent symptom of a trend strain the most is used for experiments at different levels；For sterile individual plant, enter
Row combining ability test, in combination with blast resistance identification, Fertility identification and rice quality analysis etc., is selected in long-day conditions
Under fertility critical-temperature can be reduced to 21 DEG C or so, the fertility critical-temperature under the conditions of short-day brings up to 25 DEG C of left sides
The right side, while the paddy rice japonica rice two-line sterile line that rice blast resistance is good, general combining ability is high, rice quality is excellent is identified.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
SEQUENCE LISTING
<110>Ningbo City Agriculture Science Academy
<120>It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
<130> 1
<160> 4
<170> PatentIn version 3.5
<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223>Pi2 upstream region of gene marks forward primer
<400> 1
ggtcgtgttg catcggtaag 20
<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223>Pi2 upstream region of gene marks reverse primer
<400> 2
ttcccccatc gtgcaaattg 20
<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223>Pi2 downstream of gene marks forward primer
<400> 3
tccaagactg aacagcgaga 20
<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223>Pi2 downstream of gene marks reverse primer
<400> 4
tgcgaatcaa atcactgcac 20
Claims (6)
1. it is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance, it is characterised in that：Bag
Include following steps：
(1) done and hybridized with indica type blast resisting two-line sterile line and conventional japonica rice, plantation hybrid F1Generation, molecular marker assisted selection
Positive individual plant is returned with conventional japonica rice, obtains BC1F1For seed；
(2) BC is planted1F1Generation, molecular marker assisted selection positive individual plant is returned with conventional japonica rice, while harvesting BC1F2In generation, plants
Son；
(3) BC is planted2F1Generation, molecular marker assisted selection positive individual plant is returned with conventional japonica rice, harvests BC3F1For seed；
(4) BC is planted3F1Generation, molecular marker assisted selection positive individual plant harvest BC3F2For seed；
(5) BC is planted by strain3F2Generation selection colony, carries out molecular marker assisted selection, in positive homozygosis individual plant, selection knot
The good individual plant harvest BC of real rate height, big panicle many grains per panicle, yielding ability3F3For seed, simultaneous selection thoroughly preferably, plant by infertility, flowering habit
The individual plant that strain is not higher than 85cm cuts root and stem of certain plants regeneration, and BC is harvested under short day cryogenic conditions3F3For seed.
2. it is according to claim 1 while improving the breeding side of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
Method, it is characterised in that：Indica type blast resisting two-line sterile line described in step (1) is China 1015S.
3. it is according to claim 1 while improving the breeding side of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
Method, it is characterised in that：Conventional japonica rice described in step (1) is peaceful 84 or No. 2 B of river in Zhejiang Province round-grained rice.
4. it is according to claim 1 while improving the breeding side of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
Method, it is characterised in that：Molecular marker assisted selection described in step (1)~(5) is selected to take Pi2 upstream region of gene Indel to mark
Select, Pi2 downstream of gene SSR marker selection one or both of；
The used primer information of the Pi2 upstream region of gene Indel marks selection is as follows：
Pi2 upstream region of gene marks forward primer：GGTCGTGTTGCATCGGTAAG；
Pi2 upstream region of gene marks reverse primer：TTCCCCCATCGTGCAAATTG；
The Pi2 downstream of gene SSR marker selects used primer information as follows：
Pi2 downstream of gene marks forward primer：TCCAAGACTGAACAGCGAGA；
Pi2 downstream of gene marks reverse primer：TGCGAATCAAATCACTGCAC.
5. it is according to claim 1 while improving the breeding side of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
Method, it is characterised in that：Step harvests BC described in (2)1F2After seed, quality sorting is carried out, the quality sorting is indoors
Rice quality analysis is carried out, the poor individual plant of exterior quality is eliminated and its does backcrossing combination.
6. it is according to claim 1 while improving the breeding side of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance
Method, it is characterised in that：Short day cryogenic conditions described in step (5) are 11h~12h sunshines, under the conditions of 18 DEG C~22 DEG C.
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| CN201710395372.2A CN106962185B (en) | 2017-05-26 | 2017-05-26 | Breeding method that is a kind of while improving conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance |
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Cited By (1)
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| CN108496790A (en) * | 2018-02-13 | 2018-09-07 | 湖北省农业科学院粮食作物研究所 | A method of cultivating rice anti-rice blast two-line sterile line |
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| CN106962185B (en) | 2018-11-09 |
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