CN108967185A - The selection of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting - Google Patents
The selection of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting Download PDFInfo
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Abstract
The present invention relates to a kind of selections of high-quality strong advantage Indica Rice Restorer Lines of blast resisting, belong to Crop Genetic Breeding technical field.This method is related to blast resisting, comprehensive agronomy character is excellent, advantage is strong, the good Indica Rice Restorer Lines parent one (China accounts for) of coordinate force is used as blast resisting parent, it is excellent with rice quality, not blast resisting, comprehensive agronomy character is excellent, advantage is strong, the good Indica Rice Restorer Lines parent two (R13) of coordinate force makees Quality parents, hybridization, utilize the blast resistant gene Pi2 of single plant in Markers for Detection progeny population, Pi54, Pid3, Pita and Pib (blast resistant gene that parent one is contained), the exterior quality of single plant in progeny population is investigated with Manual rice sheller, it is compared with parent two, comprehensive blast resistance identification result, exterior quality qualification result and economical character selection, select the strong advantage restorer of new high-quality blast resisting, and then from different sterile line combos, it selects high-quality Blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova, improves rice quality, the rice blast resistance of the first generation of hybrid, it is made to meet the needs of current Rice Production.
Description
Technical field
The present invention provides a kind of selection of strong advantage Indica Rice Restorer Lines of high-quality blast resisting, belongs to Crop Genetic Breeding
Technical field.
Background technique
Rice is one of China or even world's staple food crop, and hybrid rice is as the main path for improving rice yield
One of, it is widely used and promotes, make a great contribution for grain security production.High yield, it is high-quality, disease-resistant be that hybrid rice educates
Kind main target, how to make overall plans and coordinate the relationship between three, cultivate high yield, high-quality, disease-resistant indica Hybrid Rice kind, be hybridization
The main task of long-grained nonglutinous rice breeding researcher.
Application and the popularization of China hybrid rice, so that rice yield has substantially met the demand of people's life at present.With
The continuous improvement of living standard and quality of life, demand of the people to superior rice be increasingly enhanced, but it is miscellaneous due to what is promoted at present
Hand over rice variety high yield not high-quality, therefore, breeding Fine Quality Hybrid Rice kind becomes one of the main target of current rice breeding.
Rice quality is mainly by hereditary capacity and environmental condition comprehensive function as a result, but the quality characteristic of hybrid rice parents is shadow
Ring the determinant of rice quality.Hybrid rice quality character inheritance studies have shown that parents' quality trait it is excellent be select it is excellent
The effective way of matter New Hybrid Rice Combination.Fine Quality Hybrid Rice breeding, it is necessary to select parents' combo that quality is excellent, or at least
One of parents are Quality parents, and another Parent for Quality Traits should also have medium quality on the upper side.
The rice blast as caused by Pyricularia oryzae (Magnaporthe oryzae) is to threaten the safety in production of world's rice most
One of main fungal class disease, the global paddy underproduction as caused by rice blast every year reach 10%-30%.In the past 40 years, due to parent
The diversity of the narrow and highly pathogenic Pyricularia oryzae of this hereditary basis, rice blast have become hybrid rice production face mainly ask
Topic, the hybrid rice variety promoted in the recent period in addition not degenerate by blast resisting or resistance, limits hybrid rice in production
Using.It is then that prevention and treatment rice blast endangers method the most cost-effective using resistant gene, the disease-resistant New Hybrid Rice Combination of cultivation.
Restorer is the important Germplasm Bases of indica Hybrid Rice breed breeding, reinforces the strong advantage restorer choosing of high-quality blast resisting
It educates, and then prepares high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova, be to improve Hybrid Rice quality and mitigation rice blast
One of main path of harm, and meet the urgent need of people's life and Rice Production.
R13 is the Indica Rice Restorer Lines of Inst. of Agricultural Science, Lixiahe Prefecture, Jiangsu Prov.'s breeding, and rice quality is mainly physical and chemical
For index in addition to amylose content, other indexs reach national standard high quality paddy grade III Standard or more.Make male parent breeding with R13 to form
Eclectics's rice variety raise two excellent 013, rice quality is measured through Ministry of Agriculture's food quality supervision verification test center, main to manage
Change index and reaches national 3 grades of " high quality paddy " standard.China account for as Indica Rice Restorer Lines (national rice data center,
Http:// www.ricedata.cn), it is widely used in indica Hybrid Rice breeding in recent years, the cross combination of configuration is to rice blast
Show good resistance, and China accounts for itself and also shows resistance of wide spectrum to rice blast, Molecular mapping by China account for containing it is anti-
Ospc gene is positioned at Pi2/Pi9/Pi50 gene region on the 6th chromosome of rice, passes through Pi2 allelic sequences analytical proof China
It accounts for containing Pi2 gene.This research team designs molecule mark according to located or clone wide spectrum blast resistant gene information
Note detects that China accounts for simultaneously containing the blast resistant genes such as Pi54, Pid3, Pita and Pib, this also from proving it on the other hand
Resistance of wide spectrum is shown to rice blast.In modern breeding, molecular marker assisted selection (MAS) has become cultivation disease-resistant variety
A kind of main path, can increase the reliability of selection, mitigate breeding work amount and breeding cost.
The present invention is accounted for using China as disease-resistant gene donor parents, using R13 as fine quality rice character donor, utilizes molecule mark
Note detection China account for containing rice blast resistance gene Pi2, Pi54, Pid3, Pita and Pib, reinforce Breeding Process in fine quality rice
The selection of main indicator, comprehensive agronomy character determination and blast resistance identification select the new strong advantage of high-quality blast resisting
Indica Rice Restorer Lines raise extensive 508, and then configure high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova, to raising rice quality and solution
Rice blast harm has important practical application value in Rice Production.
Summary of the invention:
The purpose of the present invention is to provide a kind of selections of high-quality strong advantage Indica Rice Restorer Lines of blast resisting.
It is a further object of the present invention to provide a kind of selections of high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova.
Using blast resisting, comprehensive agronomy character is excellent, advantage is strong, coordinate force is good Indica Rice Restorer Lines as parent one, with
The Indica Rice Restorer Lines that rice quality is excellent, blast resisting, comprehensive agronomy character be not excellent, strong advantage, coordinate force are good make parent two,
Hybridization, the blast resistant genes such as Pi2, Pi54, Pid3, Pita and Pib contained using Markers for Detection parent one reinforce choosing
The selection of fine quality rice main indicator during educating, comprehensive agronomy character determination and blast resistance identification are as a result, select new
The strong advantage restorer of blast resisting, can be effectively used for high-quality, blast resisting, high-yield hybrid long-grained nonglutinous rice combination breeding, cultivate it is excellent
The new rice combination of matter blast resisting high-yield hybrid Xian.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting, comprising the following steps:
1) made with blast resisting, the Indica Rice Restorer Lines (parent one) that comprehensive agronomy character is excellent, advantage is strong, coordinate force is good female
This, makees paternal hybrid with the Indica Rice Restorer Lines (parent two) that high-quality, comprehensive agronomy character is excellent, advantage is strong, coordinate force is good, obtains
F1For seed；
2) F is planted1In generation, mixes and receives, and obtains F2Seed；
3) F plants in big group2Generation, population size >=500 plant, seedling stage pass through Markers for Detection F2Each single plant in group
Blast resistant gene (blast resistant gene that parent one is contained), investigate economical character, comprehensive Markers for Detection and agronomy
Character determination selects single-strain seed as a result, dividing in single plant harvest, after seed dries, investigates each single plant with Manual rice sheller
Exterior quality, compared with parent two, retaining head rice rate high (head rice rate >=80%), appearance transparence, good (grain of rice is saturating
Bright, non-mixed color), chalky grain rate it is low (≤15%), and exterior quality and parent two quite or better than parent two single-strain seed, build
Vertical strain F3；
4) F plants in plant division system3Generation, comprehensive Markers for Detection blast resistant gene and economical character selection result, pedigree
Breeding selects single-strain seed in harvest, continues the exterior quality for investigating each single plant with Manual rice sheller, still with parent two
It compares, retains head rice rate high (head rice rate >=80%), appearance transparence good (grain of rice transparent, non-mixed color), chalky grain rate
Low (≤15%), and exterior quality and parent two quite or better than parent two single-strain seed, establish strain F4；
5) proceed by generations Markers for Detection blast resistant gene, selection containing blast resistant gene and economical character it is excellent
Single plant, with Manual rice sheller investigate in menu strain exterior quality, step 4) is repeated, until F7Generation, in the selection from generation to generation
F is obtained with precious Xian 97A test cross containing blast resistant gene, the strain that economical character is excellent, exterior quality is good8For seed and relatively
The test cross kind answered；
6)F8For plantation superior strain and test cross kind in pairs, hybrid vigour is identified, while utilizing from rice blast resend ward
The rice disease fringe of the natural infection rice blast of field acquisition, cultivates germ, selects representative strong virus force single-ascospore strain, expands culture
Rice blast fungus spore is eluted afterwards, adjusts concentration to 2 × 105 mL-1, the inoculum used as identification, respectively to stable F8
Seedling pest, fringe pest blast resistance identification are carried out for strain, the exterior quality of each strain is still investigated with Manual rice sheller,
Synthetic heterosis, Other Main Agronomic Characters, blast resistance identification result, exterior quality are investigated as a result, selection meets breeding mesh
Target stable strain is bred as the strong advantage Indica Rice Restorer Lines of new high-quality blast resisting；
Wherein, the preferred China of parent one accounts for；The preferred R13 of parent two.
Step 3), 4), 5) in, blast resistant gene Pi2, Pi54 for containing preferably by Markers for Detection parent one,
Pid3, Pita and Pib.
Step 3), 4), 5) in preferably, begin to economical character selection from early generation.
Step 3), 4), 5), 6) in, investigate the exterior quality of each strain, with Manual rice sheller preferably with parent two
It compares, retains head rice rate high (head rice rate >=80%), appearance transparence good (grain of rice transparent, non-mixed color), chalky grain rate
Low (≤15%), and exterior quality and parent two in parent's single plant ability quite or better than selecting.
The selection it is also preferable to include:
7) expand numerous new strong advantage Indica Rice Restorer Lines seed of high-quality blast resisting, meanwhile, male parent is made with it, with different infertility
System makees female parent, configures cross combination, identifies the combo advantage of the strong advantage restorer of the high-quality blast resisting of incubation, and in boot stage
Inoculation step 6) used in Pyricularia oryzae, in different rice blast Disease garden identification field resistances, comprehensive fringe pest, sick nursery Resistance Identification knot
Fruit, overall merit are bred as restorer and with the rice blast resistances of its cross combination for making male parent configuration；Harvest cross combination
Paddy detects rice quality.
Extensive 508 are raised according to the strong advantage Indica Rice Restorer Lines of high-quality blast resisting that above method is bred as, feature are as follows: belong to
Indica type middle Xian restorer.The time of infertility 131 days or so, plant height 107.2cm, spike length 21.2cm, effective fringe 18.9 ten thousand per acre, every fringe
Total grain number 113.1, setting percentage 82.8%, 23.1 grams of mass of 1000 kernel.Rice blast composite index is 3.0, middle blast resisting.Combo is excellent
Gesture is strong: with extensive 508 and different sterile line combos are raised, yield heterosis is obvious, it is all combination all relatively control volume increase, and in anti-rice blast
Disease.The main physical and chemical index of rice quality: brown rice yield 82%, head rice rate 66.2%, chalky grain rate 15%, chalkiness degree 2.2%, directly
Chain content of starch 15.3%, gel consistence, length-width ratio 3.3, composite rating reach national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture
" edible rice varieties quality " standard high quality is third.
The breeding of high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova:
On the basis of the high-quality blast resisting of incubation strong advantage Indica Rice Restorer Lines, make maternal configuration hybridization with different sterile lines
Combination, hybrid generation are guaranteeing yield excellent compared with II 084 (the miscellaneous Xian regional testing check variety in Jiangsu Province) or rich two No. 4 excellent (country
Xian check variety in regional testing) under the premise of volume increase, boot stage with rice blast strain inoculated used in step 6), identifies hybridization group
The neck blast resistance of conjunction, in combination with different rice blast sick nursery Resistance Identifications as a result, the rice blast of the cross combination of overall merit configuration
Sick resistance；The paddy of all cross combination hybrid generations is harvested, referring to national " high quality paddy " standard, detects all cross combinations
Rice quality.Finally, high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova is obtained.
The strong advantage restorer of the high-quality blast resisting of the incubation preferably raises extensive 508.
The different rice blast sick nurseries preferably are selected from Shanghang, Fujian, Jinggangshan County, Jiangxi Province, Anhui Jinzhai County, the rice of enshi 4
Seasonal febrile diseases sick nursery.
According to the above method, extensive 508 are raised with the strong advantage Indica Rice Restorer Lines of high-quality blast resisting and makees male parent, make mother to raise Xian 9A
This combo is bred as high-quality, blast resisting, high-yield hybrid long-grained nonglutinous rice Combination nova raises Xian excellent 9508；
The feature for raising Xian excellent 9508 is: belonging to Three-line Indica Hybrid Rice kind.In the middle and lower reach of Yangtze River and Jiangsu Province
The plantation of indica Hybrid Rice area, the time of infertility 139.2 days, 084 (CK) more excellent than II was 1.7 days precocious.Plant height is 123.3 centimetres average, per acre
Number of productive ear 14.8 ten thousand, 25.2 centimetres of spike length, every total grain panicle number 180.3, setting percentage 84.9%, 27.8 grams of mass of 1000 kernel.Resistance:
3.0 grades of rice blast composite index, fringe pest loss late is 5 grades highest；5 grades of bacterial leaf-blight.Rice matter main indicator: Coarse Rice Rate
81.1%, head rice rate 68.8%, length-width ratio 3.3, chalky grain rate 15.0%, chalkiness degree 3.3%, 1 grade of transparency, caustic SCC
6.7 grades, gel consistence 82mm, amylose content 15.0%, composite rating reaches national 3 grades of " high quality paddy " standard high quality, agricultural
Portion " edible rice varieties quality " standard high quality is third.644.8 kilograms of Jiangsu Province's indica Hybrid Rice preliminary experiment per mu yield in 2017, than II
Excellent 084 (CK) volume increase 7.35%.
Beneficial effects of the present invention:
The invention patent is a kind of selection of high-quality strong advantage Indica Rice Restorer Lines of blast resisting.This method is related to benefit
The blast resistant gene contained with Markers for Detection parent one；Entire Breeding Process, through economical character selection, with paddy hand
Work sheller investigates exterior quality；Advanced lines (F8) stable strain hybrid vigour, Other Main Agronomic Characters and exterior quality are investigated
Identification, and seedling pest, fringe pest and the identification of sick nursery field resistance are carried out to it；Finally, synthetic heterosis, Other Main Agronomic Characters, outer
Quality investigation, blast resistance identification are seen as a result, being bred as the new strong advantage Indica Rice Restorer Lines of high-quality blast resisting；Utilize above-mentioned side
The restorer and different sterile line combos that method is bred as, and yield heterosis identification, rice quality detection, fringe pest inoculation are carried out to cross combination
Identification and the identification of rice blast sick nursery field resistance, are bred as high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova.Cross combination tool
It has the advantage that the rice blast resistance that (1) contains parent one passes through Markers for Detection and genetic improvement, makes it in incubation
It is expressed in restorer and Hybrid Rice Combination, and then blast resisting, it is advantageous to protect kind intellectual property；(2) entire Breeding Process is used
Manual rice sheller investigate exterior quality, the restorer of incubation, have with parent's diyl quite or better than parent two rice
Quality；(3) make female parent with different sterile lines, hybridize Xian with what the strong advantage restorer combo of the high-quality blast resisting being newly bred as was bred as
Rice Combination nova, not only blast resisting, and also rice quality is excellent.
Detailed description of the invention:
Fig. 1: for the strong advantage Indica Rice Restorer Lines breeding process pedigree schematic diagram of high-quality blast resisting of the invention
Specific embodiment:
A kind of the following is further explained with reference to the attached drawings selection of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting.
Embodiment 1:
1, breeding material
1) (parent one) is accounted for China and makees female parent.China account for be China Paddy Rice Inst cultivate blast resisting Indica Rice Restorer Lines.
The kind applies and authorizes to Plant new variety protection office of the Ministry of Agriculture, and kind power number: CNA20080059.0 can be purchased from
China Paddy Rice Inst.The kind is the elite restorer lines of China's hybrid paddy rice, and prepared cross combination has blast resisting, produces
The advantages that amount is high, quality is excellent, wide adaptability.
2) male parent is made with R13 (parent two).R13 is that the long-grained nonglutinous rice that Inst. of Agricultural Science, Lixiahe Prefecture, Jiangsu Prov. is bred as restores
System raises two excellent 412 with the Indica Hybrid Rice with Good-quality kind that it makees the incubation of male parent combo, and it is careful to have passed through country, Hubei Province, Jiangsu Province
Fixed, which applies and authorizes to Plant new variety protection office of the Ministry of Agriculture, kind power number: CNA20070116.9, commercially available
In Jiangsu gold soil Zhong Ye Co., Ltd.The kind has as high-quality strong advantage Indica Rice Restorer Lines, prepared cross combination
Best in quality, the advantages that yield is high, wide adaptability.
2, the breeding of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting, such as attached drawing 1:
1) with blast resisting, comprehensive agronomy character is excellent, advantage is strong, coordinate force is good, and Indica Rice Restorer Lines Hua Zhanzuo is maternal,
Make paternal hybrid with the Indica Rice Restorer Lines R13 that best in quality, comprehensive agronomy character is good, advantage is strong, coordinate force is good, obtains F1Generation kind
Son；
2) F is planted1In generation, mixes and receives, and obtains F2Seed；
3) F plants in big group2In generation, plants 500 plants, passes through Markers for Detection F2The blast resisting of each single plant in group
Gene (label the primer is detailed in Yunyu Wu, Ning Xiao, Ling Yu, Cunhong Pan, Yuhong Li,
Xiaoxiang Zhang,Guangqing Liu,Zhengyuan Dai,Xuebiao Pan,Aihong Li.Combination
Patterns of Major R Genes Determine the Level of Resistance to the M.oryzae
In Rice (Oryza sativa L.) .PLoS ONE, 2015,10 (6): e0126130), investigate economical character, comprehensive molecule
Label detection and economical character selection result divide in single plant harvest and select single-strain seed, after seed dries, shelled with Manual rice
Device investigates the exterior quality of each single plant, is compared with parent two, and it is saturating to retain head rice rate high (head rice rate >=80%), appearance
Lightness good (grain of rice transparent, non-mixed color), chalky grain rate are low (≤15%), and exterior quality and parent two quite or are better than parent two
Single plant 49, establish strain F3；
4) F plants in plant division system3Generation, comprehensive Markers for Detection blast resistant gene and economical character selection result, pedigree
Breeding selects single-strain seed in harvest, continues the exterior quality for investigating each single plant with Manual rice sheller, still with parent two
It compares, retains head rice rate high (head rice rate >=80%), appearance transparence good (grain of rice transparent, non-mixed color), chalky grain rate
Low (≤15%), and exterior quality and parent two are suitable or single plant 32 better than parent two, continue to establish strain F4；
5) proceed by generations Markers for Detection blast resistant gene, selection containing blast resistant gene and economical character it is excellent
Single plant, with Manual rice sheller investigate in menu strain exterior quality, step 4) is repeated, until F7Generation, in the selection from generation to generation
F is obtained with precious Xian 97A test cross containing blast resistant gene, economical character is excellent, exterior quality is good strain 108For seed and
Corresponding test cross kind；
6)F8Generation 10 strains of plantation and test cross kind in pairs, identify hybrid vigour, at the same using from Guangdong, Hubei, Hainan,
The rice blast resend ward field such as Zhejiang and Jiangsu acquires the rice disease fringe of natural infection rice blast, cultivates germ, selects 5 generations
Table strong virus force single-ascospore strain GD18-3 (B1, pathogenicity rate 65.6%), ES9-5-1 (B15, pathogenicity rate 75.0%), hnn1-1
(C1, pathogenicity rate 59.4%), ZJ3-4 (A15, pathogenicity rate 63.3%) and XK8-4-4 (G1, pathogenicity rate 37.5%) expand culture
Rice blast fungus spore is eluted afterwards, adjusts concentration to 2 × 105 mL-1, the inoculum used as identification, respectively to stable F8
Seedling pest, fringe pest blast resistance identification are carried out for strain, the exterior quality of each strain is still investigated with Manual rice sheller,
Synthetic heterosis, Other Main Agronomic Characters, blast resistance identification result, exterior quality are investigated as a result, selection meets breeding mesh
Target stable strain is bred as the strong advantage Indica Rice Restorer Lines of new high-quality blast resisting and raises extensive 508；
7) expand numerous new strong advantage Indica Rice Restorer Lines of high-quality blast resisting in Hainan and raise extensive 508 seed, meanwhile, father is made with it
This, makees maternal, configuration cross combination with different sterile lines, and the combo for the strong advantage restorer of blast resisting that positive season identification is bred as is excellent
Gesture, and in boot stage inoculation step 6) used in Pyricularia oryzae, in Shanghang, Fujian, Jinggangshan County, Jiangxi Province, Anhui Jinzhai County, enshi 4
A rice blast Disease garden identification field resistance, comprehensive fringe pest, sick nursery Resistance Identification as a result, overall merit be bred as restorer and with
The rice blast resistance of its cross combination for making male parent configuration.
Extensive 508 are raised according to the strong advantage Indica Rice Restorer Lines of high-quality blast resisting that above method is bred as, with following characteristics
Characteristic:
Feature: belong to indica type middle Xian restorer.The time of infertility 131 days or so, plant height 107.2cm, spike length 21.2cm, often
Mu effectively fringe 18.9 ten thousand, every total grain panicle number 113.1, setting percentage 82.8%, 23.1 grams of mass of 1000 kernel.Rice blast composite index is
3.0, middle blast resisting.
Combo advantage is strong: with extensive 508 and different sterile line combos are raised, yield heterosis is obvious, and all combinations all relatively compare rich
Two excellent No. four volume increase, and blast resisting.
The main physical and chemical index of rice quality: brown rice yield 82%, head rice rate 66.2%, chalky grain rate 15%, chalkiness degree
2.2%, amylose content 15.3%, gel consistence, length-width ratio 3.3.
3, the breeding of high-quality blast resisting good quality and high output double-linear hybrid rice Combination nova:
1) make male parent to raise extensive 508, make maternal combo to raise Xian 9A, it is new to be bred as high-quality, blast resisting, high-yield hybrid long-grained nonglutinous rice
Xian excellent 9508 is raised in combination.Yangzhou carry out advantage identification, boot stage be inoculated with Pyricularia oryzae, fringe pest is respectively 3.6, in anti-rice blast
Disease；Continuous 2 years progress variety comparative tests, and weighed throughout the year in rice blast such as enshi, Jiangxi Suichuan, Anhui Jinzhai County, Shanghang, Fujians
The identification of area's spontaneous induction is sent out, rice blast composite index 3.0 or so, shows excellent, rich, anti-, wide adaptability.Raise excellent 9508 ginseng of Xian
Add Jiangsu Province's indica Hybrid Rice preliminary experiment in 2017.
2) feature of Xian excellent 9508 is raised
Belong to Three-line Indica Hybrid Rice kind.It is planted in the middle and lower reach of Yangtze River and indica Hybrid Rice area of Jiangsu Province, the time of infertility
139.2 days, 084 (CK) more excellent than II was 1.7 days precocious.Plant height is 123.3 centimetres average, per acre number of productive ear 14.8 ten thousand, spike length 25.2
Centimetre, every total grain panicle number 180.3, setting percentage 84.9%, 27.8 grams of mass of 1000 kernel.Resistance: 3.0 grades of rice blast composite index, fringe
Pest loss late is 5 grades highest；5 grades of bacterial leaf-blight.Rice matter main indicator: Coarse Rice Rate 81.1%, head rice rate 68.8%, length-width ratio
3.3, chalky grain rate 15.0%, chalkiness degree 3.3%, 1 grade of transparency, 6.7 grades of caustic SCC, gel consistence 82mm, amylose content
15.0%, composite rating reaches national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture " edible rice varieties quality " standard high quality three
Deng.644.8 kilograms of Jiangsu Province's indica Hybrid Rice preliminary experiment per mu yield in 2017,084 (CK) volume increase 7.35% more excellent than II.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (11)
1. a kind of selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting characterized by comprising
The breeding of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting
1) make female parent with blast resisting, the Indica Rice Restorer Lines (parent one) that comprehensive agronomy character is excellent, advantage is strong, coordinate force is good,
Make paternal hybrid with the Indica Rice Restorer Lines (parent two) that high-quality, comprehensive agronomy character is excellent, advantage is strong, coordinate force is good, obtains F1
For seed；
2) F is planted1In generation, mixes and receives, and obtains F2Seed；
3) F plants in big group2Generation, population size >=500 plant, seedling stage pass through Markers for Detection F2Each single plant is anti-in group
Rice blast ospc gene (blast resistant gene that parent one is contained), maturity period investigate economical character, comprehensive Markers for Detection and agriculture
Skill character determination selects single-strain seed as a result, dividing in single plant harvest, after seed dries, investigates each list with Manual rice sheller
The exterior quality of strain, is compared with parent two, retains head rice rate high (head rice rate >=80%), the good (grain of rice of appearance transparence
Transparent, non-mixed color), chalky grain rate it is low (≤15%), and exterior quality and parent two quite or better than parent two single-strain seed,
Establish strain F3；
4) F plants in plant division system3Generation, comprehensive Markers for Detection blast resistant gene and economical character selection result, pedigree breeding,
Single-strain seed is selected in harvest, is continued the exterior quality for investigating each single plant with Manual rice sheller, is still opposed with parent two
According to, retain head rice rate high (head rice rate >=80%), appearance transparence good (grain of rice transparent, non-mixed color), chalky grain rate it is low (≤
15%), and exterior quality and parent two quite or better than parent two single-strain seed, establish strain F4；
5) it proceeds by generations Markers for Detection blast resistant gene, selects containing blast resistant gene and the excellent list of economical character
Strain, with Manual rice sheller investigate in menu strain exterior quality, step 4) is repeated, until F7In generation, in the generation, selection contains
The strain that blast resistant gene, economical character are excellent, exterior quality is good obtains F with precious Xian 97A test cross8For seed and corresponding
Cenospecies；
6)F8For plantation superior strain and test cross kind in pairs, hybrid vigour is identified, while utilizing and adopting from rice blast resend ward field
The rice disease fringe of the natural infection rice blast of collection cultivates germ, selects representative strong virus force single-ascospore strain, elutes after expanding culture
Rice blast fungus spore adjusts concentration to 2 × 105 mL-1, the inoculum used as identification, respectively to stable F8For strain
Seedling pest, fringe pest blast resistance identification are carried out, the exterior quality of each strain is still investigated with Manual rice sheller, synthesis is miscellaneous
Kind advantage, Other Main Agronomic Characters, blast resistance identification result, exterior quality are investigated as a result, selection meets the steady of breeding objective
Singling system is bred as the strong advantage Indica Rice Restorer Lines of new high-quality blast resisting.
2. a kind of selection of high-quality strong advantage Indica Rice Restorer Lines of blast resisting according to claim 1, feature exist
In: the parent one accounts for for China；Parent two is R13.
3. the selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting according to claim 1, it is characterised in that: step
It is rapid 3), 4), 5) in, blast resistant gene Pi2, Pi54, Pid3, Pita for being contained using Markers for Detection parent one (China accounts for)
And Pib.
4. the selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting according to claim 1, it is characterised in that: step
It is rapid 3), 4), 5) in preferably, begin to economical character selection from early generation.
5. the selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting according to claim 1, it is characterised in that: step
It is rapid 3), 4), 5), 6) in, the exterior quality of each single plant is investigated with Manual rice sheller, is compared, has been retained with parent two
Head rice yield height (head rice rate >=80%), appearance transparence good (grain of rice transparent, non-mixed color), chalky grain rate are low (≤15%), and
Exterior quality and parent two in the single plant ability of parent two quite or better than selecting.
6. the selection of the strong advantage Indica Rice Restorer Lines of blast resisting according to claim 1 or 2, it is characterised in that: institute
The selection stated further include:
7) expand numerous new strong advantage Indica Rice Restorer Lines seed of high-quality blast resisting, meanwhile, male parent is made with it, with different sterile lines work
Female parent configures cross combination, identifies the combo advantage of the strong advantage restorer of the high-quality blast resisting of incubation, and be inoculated in boot stage
Pyricularia oryzae used in step 6), in different rice blast Disease garden identification field resistances, comprehensive fringe pest, sick nursery Resistance Identification as a result,
Overall merit is bred as restorer and with the rice blast resistance of its cross combination for making male parent configuration；Harvest the rice of cross combination
Paddy detects rice quality.
7. the selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting according to claim 1, it is characterised in that: root
Extensive 508 are raised according to the strong advantage Indica Rice Restorer Lines of high-quality blast resisting that above method is bred as, feature are as follows: it is extensive to belong to indica type middle Xian
Multiple system.The time of infertility 131 days or so, plant height 107.2cm, spike length 21.2cm, effective fringe 18.9 ten thousand per acre, every total grain panicle number
113.1, setting percentage 82.8%, 23.1 grams of mass of 1000 kernel.Rice blast composite index is 3.0, middle blast resisting.Combo advantage is strong:
With extensive 508 and different sterile line combos are raised, yield heterosis is obvious, all combinations all relatively control volume increase, and middle blast resisting.Rice
The main physical and chemical index of quality: brown rice yield 82%, head rice rate 66.2%, chalky grain rate 15%, chalkiness degree 2.2%, amylose
Content 15.3%, gel consistence, length-width ratio 3.3, composite rating reach national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture " edible rice
Variety and quality " standard high quality is third.
8. a kind of selection of the breeding of high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova, it is characterised in that: according to power
Benefit require 1 described in selection be bred as on the basis of the high-quality strong advantage restorer of blast resisting, made with different sterile lines maternal
Configure cross combination, hybrid generation is guaranteeing yield compared with compared with II excellent 084 (trying check variety in the miscellaneous area Xian of Jiangsu Province) or rich two excellent No. 4
Under the premise of (Xian check variety in national region test) volume increase, boot stage is with rice blast strain inoculated used in step 6), identification
The neck blast resistance of cross combination, in combination with different rice blast sick nursery Resistance Identifications as a result, the cross combination of overall merit configuration
Rice blast resistance；The paddy of all cross combination hybrid generations is harvested, referring to national " high quality paddy " standard, is detected all miscellaneous
Hand over combined rice quality.Finally, high-quality blast resisting high-yield hybrid long-grained nonglutinous rice Combination nova is obtained.
9. selection according to claim 8, it is characterised in that the selection described in accordance with the claim 1
Being bred as the strong advantage restorer of high-quality blast resisting is to raise extensive 508.
10. selection according to claim 8, it is characterised in that the different rice blast sick nurseries are selected from Fujian
Hangzhoupro, Jinggangshan County, Jiangxi Province, Anhui Jinzhai County, the rice blast sick nursery of enshi 4.
11. the selection of the strong advantage Indica Rice Restorer Lines of high-quality blast resisting according to claim 1 to 10,
It is characterized by: raising extensive 508 according to the above method with the strong advantage Indica Rice Restorer Lines of blast resisting and making male parent, make mother to raise Xian 9A
This combo is bred as high-quality blast resisting, high-yield hybrid long-grained nonglutinous rice Combination nova raises Xian excellent 9508；
The feature for raising Xian excellent 9508 is: belonging to Three-line Indica Hybrid Rice kind.Hybridize in the middle and lower reach of Yangtze River and Jiangsu Province
Indica Rice Region plantation, the time of infertility 139.2 days, 084 (CK) more excellent than II was 1.7 days precocious.Plant height is 123.3 centimetres average, per acre effectively
Spike number 14.8 ten thousand, 25.2 centimetres of spike length, every total grain panicle number 180.3, setting percentage 84.9%, 27.8 grams of mass of 1000 kernel.Resistance: rice blast
3.0 grades of sick composite index, fringe pest loss late are 5 grades highest；5 grades of bacterial leaf-blight.Rice matter main indicator: Coarse Rice Rate 81.1%, it is whole
Polished rice rate 68.8%, length-width ratio 3.3, chalky grain rate 15.0%, chalkiness degree 3.3%, 1 grade of transparency, 6.7 grades of caustic SCC, glue is thick
82mm, amylose content 15.0% are spent, composite rating reaches national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture's " edible rice product
Kind of quality " standard high quality is third.644.8 kilograms of Jiangsu Province's indica Hybrid Rice preliminary experiment per mu yield in 2017,084 (CK) more excellent than II increase
Produce 7.35%.
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| CN114793890A (en) * | 2022-03-17 | 2022-07-29 | 重庆市农业科学院 | Efficient breeding method for two-line and three-line strong-advantage restorer line |
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| CN114375828A (en) * | 2021-12-03 | 2022-04-22 | 上海市农业科学院 | Breeding method of new high-quality national-Qing japonica rice variety |
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