CN108967185B - Breeding method of high-quality rice blast resistance strong-advantage indica rice restorer line - Google Patents

Breeding method of high-quality rice blast resistance strong-advantage indica rice restorer line Download PDF

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CN108967185B
CN108967185B CN201811024122.9A CN201811024122A CN108967185B CN 108967185 B CN108967185 B CN 108967185B CN 201811024122 A CN201811024122 A CN 201811024122A CN 108967185 B CN108967185 B CN 108967185B
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李育红
李爱宏
肖宁
吴云雨
戴正元
刘广青
潘存红
周长海
余玲
黄年生
张小祥
季红娟
王闯
王志平
吴诗清
李响
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JIANGSU LIXIAHE REGION AGRICULTURAL RESEARCH INSTITUTE
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Abstract

The invention relates to a breeding method of a high-quality indica rice restorer line with rice blast resistance and strong superiority. The method comprises crossing Huazhan as rice blast resistant parent and R13 as high-quality parent, and detecting rice blast resistant gene of single plant in progeny population by using molecular markerPi2、Pi54、Pid3、PitaAndPib(the first parent contains rice blast resistance genes), the appearance quality of a single plant in a progeny population is inspected by using a manual rice huller, the second parent is used as a contrast, a rice blast resistance identification result, an appearance quality identification result and agronomic character selection are integrated, a new high-quality rice blast resistance strong-dominance restoring line is bred, and then the restoring line is matched with different sterile lines to breed a new high-quality rice blast resistance high-yield hybrid indica rice combination, so that the rice quality and the rice blast resistance of the first hybrid generation are improved, and the requirements of current rice production are met.

Description

Breeding method of high-quality rice blast resistance strong-advantage indica rice restorer line
Technical Field
The invention provides a breeding method of a high-quality rice blast-resistant strong-superiority indica rice restorer line, belonging to the technical field of crop genetic breeding.
Background
The rice is one of the main grain crops in China and even in the world, and the hybrid rice is widely applied and popularized as one of the main ways of improving the yield of the rice, thereby making great contribution to the safe production of the grain. High yield, high quality and disease resistance are main targets of hybrid rice breeding, how to comprehensively coordinate the relationship among the three and cultivate high yield, high quality and disease resistance hybrid indica rice varieties is a main task of hybrid indica rice breeding researchers.
The application and popularization of hybrid rice in China enable the current rice yield to basically meet the living demands of people. With the continuous improvement of living standard and living quality, the demand of people for high-quality rice is increasingly strengthened, but because the hybrid indica rice varieties popularized at present have high yield and poor quality, the breeding of high-quality hybrid rice varieties becomes one of the main targets of the current rice breeding. The quality of rice is mainly a result of the comprehensive action of genetic characteristics and environmental conditions, but the quality characteristics of parents of hybrid rice are determining factors influencing the quality of rice. The genetic research of the quality traits of hybrid rice shows that the genetic research of the quality traits of the parents shows that the quality traits of the parents are both excellent, and the genetic research is an effective way for breeding new combinations of high-quality hybrid rice. In the breeding of high-quality hybrid rice, the parents with uniform and excellent quality must be selected, or at least one of the parents is a high-quality parent, and the quality of the other parent should have medium and superior quality.
Is prepared from rice blast fungusMagnaporthe oryzae) The caused rice blast is one of the most main fungal diseases threatening the safe production of rice in the world, and the yield of rice is reduced by 10 to 30 percent due to the rice blast every year in the world. In recent 40 years, due to the narrow genetic basis of parents and the diversity of highly pathogenic rice blast germs, rice blast has become a main problem for hybrid rice production, and the hybrid rice variety popularized recently does not resist rice blast or has resistance degeneration, so that the application of hybrid rice in production is limited. The new combination of resistance genes and the cultivation of disease-resistant hybrid rice is the most economic and effective method for preventing and treating the rice blast.
The restorer line is an important germplasm basis for breeding hybrid indica rice varieties, enhances the breeding of the restorer line with high quality and strong rice blast resistance and superiority, further prepares a new combination of high-quality and high-yield hybrid indica rice with high rice blast resistance, is one of main ways for improving the quality of the hybrid rice and reducing the damage of the rice blast, and also meets the urgent requirements of people on life and rice production.
R13 is indica rice restorer line bred by agricultural science research institute in Ri and Chi river of Jiangsu, and the main physical and chemical indexes of the rice quality of the indica rice restorer line except the amylose content all reach the third-level standard of national standard high-quality rice. The hybrid indica rice variety Yangliangyou 013 bred by using R13 as male parent, the rice quality of which is supervised and tested by food quality of Ministry of agricultureThe main physical and chemical indexes reach the level 3 of the national high-quality rice standard by the measurement of a test center. Huazhan is used as an indica rice restorer line (national rice data center, http:// www. ricedata. cn), and is widely applied to hybrid indica rice breeding in recent years, a configured hybrid combination shows good resistance to rice blast, Huazhan also shows broad-spectrum resistance to rice blast, and molecular marker positioning positions a disease-resistant gene contained in Huazhan on the 6 th chromosome of ricePi2 /Pi9/Pi50Gene region by Pi2Allelic sequence analysis demonstrates Huazhan containsPi2A gene. The research team designs molecular markers according to the information of the broad-spectrum rice blast resistance gene which is positioned or cloned, and detects that Huazhan simultaneously containsPi54、Pid3、PitaAndPiband the rice blast resistance gene, which also proves from another aspect that it shows broad-spectrum resistance to rice blast. In modern breeding, molecular Marker Assisted Selection (MAS) has become a main approach for breeding disease-resistant varieties, which can increase the reliability of selection and reduce the breeding workload and the breeding cost.
The invention uses Huazhan as disease-resistant gene donor parent, uses R13 as high-quality rice character donor, and utilizes molecular marker to detect rice blast resistance gene contained in HuazhanPi2、Pi54、Pid3、PitaAndPibthe selection of main indexes of high-quality rice is enhanced in the breeding process, the agronomic character selection and the rice blast resistance identification are integrated, a new high-quality rice blast-resistant strong-advantage indica rice restorer line Yanghui 508 is bred, and then a new high-quality rice blast-resistant high-yield hybrid indica rice combination is configured, so that the rice quality improvement and the rice blast hazard solving in rice production have important practical application values.
Disclosure of Invention
The invention aims to provide a breeding method of a high-quality rice blast-resistant strong-superiority indica rice restorer line.
Another purpose of the invention is to provide a breeding method of new high-yield hybrid indica rice combination with high quality and rice blast resistance.
Uses the indica rice restorer line with rice blast resistance, excellent comprehensive agronomic character, strong superiority and good combining ability as a parent I, and uses the rice with excellent qualityThe indica rice restorer line with good and no rice blast resistance, excellent comprehensive agronomic characters, strong superiority and good combining ability is used as a second parent, hybridization is carried out, and molecular markers are utilized to detect the indica rice restorer line contained in the first parentPi2、Pi54、Pid3、PitaAndPibthe rice blast resistance gene is used for enhancing the selection of main indexes of high-quality rice in the breeding process, and the agronomic character selection and the rice blast resistance identification result are integrated to breed a new rice blast resistance strong advantage restoring line, so that the rice blast resistance strong advantage restoring line can be effectively used for breeding high-quality, rice blast resistance and high-yield hybrid indica rice combinations and breeding high-quality, rice blast resistance and high-yield hybrid indica rice new rice combinations.
The purpose of the invention can be realized by the following technical scheme:
a breeding method of a high-quality rice blast resistance strong-superiority indica rice restorer line comprises the following steps:
1) f is obtained by hybridizing a rice restoring line (parent I) which resists rice blast, has excellent comprehensive agronomic characters, strong advantages and good combining ability as a female parent and a rice restoring line (parent II) which has excellent quality, excellent comprehensive agronomic characters, strong advantages and good combining ability as a male parent1Seed generation;
2) planting F1Collecting to obtain F2Seeds;
3) large population planting F2Generation, population scale is more than or equal to 500, and seedling stage is detected by molecular marker F2The rice blast resistance gene of each single plant in the population (the rice blast resistance gene contained in the first parent) is inspected, the agronomic characters are inspected, the molecular marker detection and the agronomic character selection results are integrated, the single plant seeds are harvested in a single plant division mode, after the seeds are dried in the air, the appearance quality of each single plant is inspected by using a manual rice huller, the second parent is used as a contrast, the single plant seeds with high complete rice rate (the complete rice rate is more than or equal to 80 percent), good appearance transparency (the rice grains are transparent and have no color mixing), low chalky grain rate (less than or equal to 15 percent) and appearance quality equivalent to or superior to that of the second parent are reserved, and a plant F is established3；
4) Planting in different lines F3Generation, comprehensive molecular marker detection of rice blast resisting gene and agricultural character selection result, genealogical selection, harvesting single plant seed, and further examination with manual rice hullerThe appearance quality of each single plant is still compared with that of the second parent, the single plant seeds with high complete rice rate (the complete rice rate is more than or equal to 80%), good appearance transparency (rice grains are transparent and have no color mixture) and low chalky grain rate (less than or equal to 15%) and the appearance quality equivalent to or superior to that of the second parent are kept, and a strain F is established4；
5) Carrying out molecular marker detection on rice blast resistance genes generation by generation, selecting individual plants containing the rice blast resistance genes and having excellent agronomic characters, observing the appearance quality of the selected individual plants by using a manual rice huller, and repeating the step 4) until F7Selecting a strain containing rice blast resistance genes, having excellent agronomic characters and good appearance quality in the generation, and carrying out test cross on the selected strain by using Zhenxian 97A to obtain F8Generation seeds and corresponding test cross seeds;
6）F8generating pair planting optimized strain and test cross species, identifying heterosis, utilizing rice ear of rice naturally infected with rice blast collected from rice blast re-infected disease area field, culturing germ, selecting representative virulent monospore strain, expanding culture, eluting rice blast spore, regulating concentration to 2X 105each.mL-1As inoculum for identification, respectively, against stable F8Carrying out seedling blast and panicle blast resistance identification on the generation lines, still using a manual rice huller to inspect the appearance quality of each line, integrating heterosis, main agronomic characters, rice blast resistance identification results and appearance quality inspection results, selecting stable lines meeting breeding targets, and breeding into a new high-quality rice blast resistance strong-dominance indica rice restorer;
wherein, the parent is preferably Huazhan; parent two is preferably R13.
In the steps 3), 4) and 5), preferably, molecular markers are used for detecting rice blast resistance genes contained in parent strainsPi2、Pi54、Pid3、PitaAndPib。
preferably, in steps 3), 4), 5), agronomic trait selection is started from an early generation.
In the steps 3), 4), 5) and 6), preferably, a manual rice huller is used for inspecting the appearance quality of each strain, the second parent is used as a reference, the high complete rice rate (the complete rice rate is more than or equal to 80%), the good appearance transparency (the rice grains are transparent and have no color mixing), the chalky grain rate is low (less than or equal to 15%) and the appearance quality is equal to or superior to that of the second parent and the single parent can be selected.
The breeding method preferably further comprises the following steps:
7) expanding and propagating new high-quality rice blast resistant strong dominant indica rice restorer line seeds, simultaneously, taking the indica rice restorer line seeds as a male parent and taking different sterile lines as female parents to configure a hybridization combination, identifying the grouping advantages of the bred high-quality rice blast resistant strong dominant restorer line, inoculating rice blast germs used in the step 6) at the booting stage, identifying field resistance in different rice blast disease gardens, integrating the rice blast and disease garden resistance identification results, and comprehensively evaluating the rice blast resistance of the bred restorer line and the hybridization combination configured by taking the indica rice restorer line seeds as the male parent; harvesting the rice of the hybrid combination, and detecting the quality of the rice.
The high-quality rice blast-resistant strong-superiority indica rice restorer line Yanghui 508 bred according to the method has the characteristic characteristics that: belongs to the restoring line of indica type. The whole growth period is about 131 days, the plant height is 107.2cm, the ear length is 21.2cm, the effective ear per mu is 18.9 ten thousand, the total number of the ears per mu is 113.1, the seed setting rate is 82.8 percent, and the thousand-grain weight is 23.1 g. The comprehensive index of the rice blast is 3.0, and the rice blast is resisted. The matching advantages are strong: the Yanghui 508 is matched with different sterile lines, the yield advantage is obvious, all combinations are increased compared with the contrast, and the rice blast resistance is achieved. The main physical and chemical indexes of the rice quality are as follows: the rice flour has the advantages of 82% of coarseness, 66.2% of whole rice, 15% of chalky grain rate, 2.2% of chalky degree, 15.3% of amylose content, 3.3% of gel consistency and aspect ratio, and the comprehensive rating reaches the national high-quality grade 3 of the high-quality rice standard, the agricultural department of the high-quality grade three of the edible rice variety quality standard.
Breeding a new combination of high-quality rice blast-resistant high-yield hybrid indica rice:
on the basis of breeding a high-quality rice blast resistance strong-dominance indica rice restorer line, different sterile lines are used as female parents to configure a hybrid combination, the rice blast strains used in the step 6) are inoculated in the booting stage on the premise of ensuring that the yield of hybrid offspring is increased compared with that of II excellent 084 (hybrid indica regional test control variety in Jiangsu province) or Fengshiyou No. 4 (indica control variety in national regional test), the panicle blast resistance of the hybrid combination is identified, and meanwhile, the rice blast resistance of the configured hybrid combination is comprehensively evaluated by combining the resistance identification results of different rice blast disease gardens; and harvesting the rice of hybrid progeny of all the hybrid combinations, and detecting the rice quality of all the hybrid combinations according to the national standard of high-quality rice. Finally, the new combination of high-quality rice blast-resistant high-yield hybrid indica rice is obtained.
The bred high-quality rice blast resistant strong-advantage restoring line preferably has Yanhui 508.
The different rice blast disease gardens are preferably selected from 4 rice blast disease gardens of Hangzhou in Fujian, Jinggang mountain in Jiangxi, Jinzhai in Anhui and Enshi in Hubei.
According to the method, a new combination of high-quality, rice blast-resistant and high-yield hybrid indica rice Yangxiao 9508 is bred by taking the high-quality rice blast-resistant strong-superiority indica rice restorer Yanghui 508 as a male parent and Yangxian 9A as a female parent;
the Yangxiao 9508 is characterized in that: belongs to indica type three-line hybrid rice varieties. The hybrid indica rice is planted in the middle and lower reaches of Yangtze river and Jiangsu province, the whole growing period is 139.2 days, and the hybrid indica rice is earlier ripe than II-084 (CK) for 1.7 days. The average height of the plant is 123.3 cm, the number of effective spikes per mu is 14.8 ten thousand, the spike length is 25.2 cm, the total number of the spikes is 180.3, the seed setting rate is 84.9%, and the thousand seed weight is 27.8 g. Resistance: the rice blast comprehensive index is 3.0 grade, and the highest panicle blast loss rate is 5 grade; bacterial leaf blight 5 grade. The main indexes of the rice quality are as follows: the brown rice rate is 81.1 percent, the whole rice rate is 68.8 percent, the aspect ratio is 3.3, the chalkiness grain rate is 15.0 percent, the chalkiness degree is 3.3 percent, the transparency is grade 1, the alkali digestion value is grade 6.7, the glue consistency is 82mm, the amylose content is 15.0 percent, the comprehensive rating reaches the national high-quality rice standard grade 3, the Ministry of agriculture is high-quality rice variety quality standard grade three, and the like. The yield per mu of the hybrid indica rice in Jiangsu province in 2017 is 644.8 kg, which is 7.35% higher than that of II You 084 (CK).
The invention has the beneficial effects that:
the invention discloses a breeding method of a high-quality indica rice restorer line with rice blast resistance and strong superiority. The method involves detecting a rice blast resistance gene contained in a parent by using a molecular marker; throughout the whole breeding process, through agronomic character selection, appearance quality is inspected by using a manual rice huller; advanced generation (F)8) For stable line heterosis, main agricultureInspecting and identifying the characters and appearance quality, and performing field resistance identification on the seedlings plague, the panicle plague and the disease nursery; finally, combining the heterosis, main agronomic characters, appearance quality investigation and rice blast resistance identification results to breed a new high-quality rice blast-resistant strong-dominance indica rice restorer; the restorer line bred by the method is matched with different sterile lines, and the hybrid combination is subjected to yield advantage identification, rice quality detection, panicle blast inoculation identification and rice blast disease garden field resistance identification to breed the high-quality rice blast-resistant high-yield hybrid indica rice new combination. The hybrid combination has the following advantages: (1) the rice blast resistance contained in the first parent is expressed in the bred restorer line and hybrid rice combination through molecular marker detection and genetic improvement, so that the rice blast resistance is resistant to rice blast and the intellectual property of varieties is protected; (2) the appearance quality is inspected by a manual rice huller in the whole breeding process, and the bred restoring line has rice quality equivalent to or superior to that of the second parent group; (3) the new combination of hybrid indica rice bred by using different sterile lines as female parents and newly bred high-quality rice blast resistant strong-dominance restoring line not only can resist rice blast, but also has good rice quality.
Description of the drawings:
FIG. 1: is a pedigree schematic diagram of the breeding process of the high-quality rice blast-resistant strong-superiority indica rice restorer line.
The specific implementation mode is as follows:
the breeding method of the high-quality rice blast-resistant strong-superiority indica rice restorer line is further described with reference to the accompanying drawings.
Example 1:
1. breeding material
1) Huazhan (parent I) is used as a female parent. Huazhan is a rice blast resistant indica rice restorer line cultivated by the Chinese rice institute. The variety has been applied and authorized to the new plant variety protection office of Ministry of agriculture, and the variety right number: CNA20080059.0, available from Rice research institute of China. The variety is a backbone restoring line of hybrid rice in China, and the prepared hybrid combination has the advantages of rice blast resistance, high yield, high quality, wide adaptability and the like.
2) R13 (parent II) is used as a male parent. R13 is indica rice restorer line bred by agricultural science institute of Ri.Ching, and high-quality hybrid indica rice breed Yangliou 412 bred by matching with the indica rice restorer line as male parent passes the approval of the nation, Hubei province and Jiangsu province, and is applied and authorized to the protection office of new plant varieties of Ministry of agriculture, and the breed right number is as follows: CNA20070116.9, available from Jiangsu gold land species industry Co. The variety is used as a high-quality strong-superiority indica rice restorer line, and the prepared hybrid combination has the advantages of excellent quality, high yield, wide adaptability and the like.
2. The breeding of the high-quality rice blast-resistant strong-superiority indica rice restorer line is as shown in the attached figure 1:
1) f is obtained by hybridizing a rice blast resistant indica rice restorer line Huazhan as female parent with excellent comprehensive agronomic characters, strong superiority and good combining ability and a indica rice restorer line R13 as male parent with excellent quality, good comprehensive agronomic characters, strong superiority and good combining ability1Seed generation;
2) planting F1Collecting to obtain F2Seeds;
3) large population planting F2Planting 500 plants, detecting F by molecular marker2Rice blast Resistance Genes for each individual plant in the population (primers used for labeling are described in Yunyu Wu, Ning Xiao, Ling Yu, Cunhong Pan, Yuhong Li, Xiaoxing Zhang, Guangqing Liu, Zhengyuan Dai, Xuebiao Pan, Aihong Li. Combination Patters of Major R Genes determination the Level of Resistance to the M.oryzae in Rice. PLoS ONE2015, 10(6): e 0126130), examining agronomic characters, integrating molecular marker detection and agronomic character selection results, selecting seeds from individual plants, harvesting the seeds separately, after the seeds are dried in the air, examining the appearance quality of each individual plant by using a manual rice huller, using the second parent as a contrast, keeping high complete rice rate (the complete rice rate is more than or equal to 80%), good appearance transparency (rice grains are transparent and have no color mixing), low chalky grain rate (less than or equal to 15%), and 49 individual plants with appearance quality equivalent to or superior to that of the second parent, and establishing a plant system F3；
4) Planting in different lines F3Generation, comprehensive molecular marker detection of rice blast resisting gene and agricultural character selection result, genealogical selective breeding, harvesting single plant seed, and further usingThe manual rice huller examines the appearance quality of each single plant, the two parents are still used as a reference, the full rice rate is high (the full rice rate is more than or equal to 80 percent), the appearance transparency is good (the rice grains are transparent and have no color mixture), the chalky grain rate is low (less than or equal to 15 percent), the appearance quality is equivalent to or superior to that of the two parents, 32 single plants are continuously established, and the plant system F is continuously established4；
5) Carrying out molecular marker detection on rice blast resistance genes generation by generation, selecting individual plants containing the rice blast resistance genes and having excellent agronomic characters, observing the appearance quality of the selected individual plants by using a manual rice huller, and repeating the step 4) until F7Selecting 10 lines with rice blast resistance gene, excellent agronomic character and good appearance quality in the generation, and testing cross with Zhenxian 97A to obtain F8Generation seeds and corresponding test cross seeds;
6）F8generating 10 strains in pairs, planting test cross species, identifying heterosis, simultaneously collecting rice ears naturally infected with rice blast from rice blast retransmission disease areas such as Guangdong, Hubei, Hainan, Zhejiang and Jiangsu, culturing germs, selecting 5 representative virulent monospore strains GD18-3 (B1, the pathogenic rate is 65.6%), ES9-5-1 (B15, the pathogenic rate is 75.0%), hnn1-1 (C1, the pathogenic rate is 59.4%), ZJ3-4 (A15, the pathogenic rate is 63.3%) and XK8-4-4 (G1, the pathogenic rate is 37.5%), eluting rice blast spores after expanding culture, adjusting the concentration to 2 x 105. mL-1, and respectively serving as inoculants for identification, wherein the stable F strains are respectively subjected to stable F infection8Carrying out resistance identification on seedling blast and panicle blast rice blast of the generation strains, still using a manual rice huller to inspect the appearance quality of each strain, integrating heterosis, main agronomic characters, rice blast resistance identification results and appearance quality inspection results, selecting stable strains meeting breeding targets, and breeding into a new high-quality rice blast resistant strong-dominance indica rice restorer Yanghui 508;
7) propagating new high-quality rice blast resistant strong-dominance indica rice restorer Yanghui 508 seeds in Hainan, simultaneously, taking the hybrid combination as a male parent and different sterile lines as female parents, identifying the grouping dominance of the bred rice blast resistant strong-dominance restorer in the season, inoculating the rice blast germs used in the step 6) in the booting stage, identifying the field resistance in 4 rice blast disease gardens of Hangzhou, Jiangxi Jinggang mountain, Anhui Jinzhai and Hunbei in Fujian, synthesizing the rice blast resistance identification results of the bred restorer and the hybrid combination prepared by taking the hybrid combination as the male parent.
The high-quality rice blast-resistant strong-superiority indica rice restorer line Yanghui 508 bred according to the method has the following characteristic characteristics:
characteristic features: belongs to the restoring line of indica type. The whole growth period is about 131 days, the plant height is 107.2cm, the ear length is 21.2cm, the effective ear per mu is 18.9 ten thousand, the total number of the ears per mu is 113.1, the seed setting rate is 82.8 percent, and the thousand-grain weight is 23.1 g. The comprehensive index of the rice blast is 3.0, and the rice blast is resisted.
The matching advantages are strong: the Yanghui 508 is matched with different sterile lines, the yield advantage is obvious, all combinations are increased compared with the Fengliouyou No. 4, and the rice blast resistance is achieved.
The main physical and chemical indexes of the rice quality are as follows: the coarseness of the rice was 82%, the whole rice percentage of 66.2%, the chalky grain percentage of 15%, the chalky degree of 2.2%, the amylose content of 15.3%, the consistency of the gum, the aspect ratio of 3.3.
3. The new combination breeding of the high-quality rice blast-resistant high-quality high-yield two-line hybrid rice:
1) the new hybrid indica rice combination Yanghui 9508 with high quality, rice blast resistance and high yield is bred by taking Yanghui 508 as a male parent and Yanghui 9A as a female parent. Performing advantage identification in Yangzhou, inoculating rice blast germs in the booting stage, wherein the panicle blast is 3.6 respectively, and resisting rice blast; the quality ratio test is continuously carried out for two years, and the perennial reissued areas of rice blast such as Hung-Shi, Jiangxi Tuchuan, Anhui Jinzhai, Hangzhou in Fujian and the like are naturally induced and identified, the comprehensive indexes of the rice blast are all about 3.0, and the rice blast has excellent performance, abundance, resistance and wide adaptability. Yangxiao you 9508 participates in the preparation test of hybrid indica rice in Jiangsu province in 2017.
2) Characteristic characteristics of Yangxiao you 9508
Belongs to indica type three-line hybrid rice varieties. The hybrid indica rice is planted in the middle and lower reaches of Yangtze river and Jiangsu province, the whole growing period is 139.2 days, and the hybrid indica rice is earlier ripe than II-084 (CK) for 1.7 days. The average height of the plant is 123.3 cm, the number of effective spikes per mu is 14.8 ten thousand, the spike length is 25.2 cm, the total number of the spikes is 180.3, the seed setting rate is 84.9%, and the thousand seed weight is 27.8 g. Resistance: the rice blast comprehensive index is 3.0 grade, and the highest panicle blast loss rate is 5 grade; bacterial leaf blight 5 grade. The main indexes of the rice quality are as follows: the brown rice rate is 81.1 percent, the whole rice rate is 68.8 percent, the aspect ratio is 3.3, the chalkiness grain rate is 15.0 percent, the chalkiness degree is 3.3 percent, the transparency is grade 1, the alkali digestion value is grade 6.7, the glue consistency is 82mm, the amylose content is 15.0 percent, the comprehensive rating reaches the national high-quality rice standard grade 3, the Ministry of agriculture is high-quality rice variety quality standard grade three, and the like. The yield per mu of the hybrid indica rice in Jiangsu province in 2017 is 644.8 kg, which is 7.35% higher than that of II You 084 (CK).
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims (6)

1. A breeding method of a high-quality rice blast resistance strong-superiority indica rice restorer line is characterized by comprising the following steps: 1) f is obtained by hybridizing a rice blast resistant indica rice restorer line Huazhan with excellent comprehensive agronomic characters, strong superiority and good combining ability as a female parent and a high-quality indica rice restorer line R13 with excellent comprehensive agronomic characters, strong superiority and good combining ability as a male parent1Seed generation;
2) planting F1Collecting to obtain F2Seeds;
3) large population planting F2Generation, population scale is more than or equal to 500, and seedling stage is detected by molecular marker F2Rice blast resistance gene of each individual plant in the colony, examining agronomic characters in the mature period, integrating molecular marker detection and agronomic character selection results, selecting individual plant seeds in individual plant harvest, after the seeds are dried in the air, examining the appearance quality of each individual plant by using a manual rice huller, using indica rice restorer R13 as a contrast, keeping the complete rice rate more than or equal to 80%, rice grains transparent, no color mixing, chalky grain rate less than or equal to 15%, and establishing a plant line F of the individual plant seeds with the appearance quality equal to or superior to indica rice restorer R13 or superior to indica rice restorer R133；
4) Planting in different lines F3Generation, detecting the result of rice blast resistance gene and agronomic character selection by integrating molecular markers, performing genealogical selection, harvesting seeds of single plants, continuously using a manual rice huller to examine the appearance quality of each single plant, and observing the appearance quality of each single plant according to the result of the manual rice hullerThen using indica rice restorer R13 as a reference, keeping the single plant seeds with the complete rice rate of more than or equal to 80 percent, transparent rice grains, no mixed color, chalky grain rate of less than or equal to 15 percent, and the appearance quality equivalent to or superior to indica rice restorer R13, and establishing a strain F4；
5) Carrying out molecular marker detection on rice blast resistance genes generation by generation, selecting individual plants containing the rice blast resistance genes and having excellent agronomic characters, observing the appearance quality of the selected individual plants by using a manual rice huller, and repeating the step 4) until F7Selecting a strain containing rice blast resistance genes, having excellent agronomic characters and good appearance quality in the generation, and carrying out test cross on the selected strain by using Zhenxian 97A to obtain F8Generation seeds and corresponding hybrid seeds;
6）F8generating pair planting optimized strain and test cross species, identifying heterosis, utilizing rice ear of rice naturally infected with rice blast collected from rice blast re-infected disease area field, culturing germ, selecting representative virulent monospore strain, expanding culture, eluting rice blast spore, regulating concentration to 2X 105each.mL-1As inoculum for identification, respectively, against stable F8Carrying out seedling blast and panicle blast resistance identification on the generation lines, still using a manual rice huller to inspect the appearance quality of each line, integrating heterosis, main agronomic characters, rice blast resistance identification results and appearance quality inspection results, selecting stable lines meeting breeding targets, and breeding into a new high-quality rice blast resistance strong-dominance indica rice restorer;
wherein, in the steps 3), 4) and 5), the molecular marker is used for detecting the rice blast resistance gene contained in the HuazhanPi2、Pi54、Pid3、PitaAndPib。
2. the breeding method of the high-quality rice blast-resistant strong-dominance indica rice restorer line according to claim 1, characterized in that: step 3), 4), 5), starting agronomic trait selection from the early generation.
3. The breeding method of the high-quality rice blast-resistant strong-dominance indica rice restorer line according to claim 1, characterized in that: in the steps 3), 4), 5) and 6), the appearance quality of each single plant is inspected by using a rice manual huller, and compared with indica rice restorer R13, the rice manual huller is high in complete rice rate, good in appearance transparency and low in chalkiness grain rate, the appearance quality of each single plant is equivalent to that of indica rice restorer R13 or superior to that of indica rice restorer R13, the complete rice rate is high and means that the complete rice rate is more than or equal to 80%, the appearance transparency means that rice grains are transparent and have no color mixture, and the chalkiness grain rate is low and means that the chalkiness grain rate is less than or equal to 15%.
4. The breeding method of the rice blast resistant strong dominant indica rice restorer line according to claim 1, characterized in that: the breeding method further comprises the following steps:
7) expanding and propagating new high-quality rice blast resistant strong dominant indica rice restorer line seeds, simultaneously, taking the indica rice restorer line seeds as a male parent and taking different sterile lines as female parents to configure a hybridization combination, identifying the grouping advantages of the bred high-quality rice blast resistant strong dominant restorer line, inoculating rice blast germs used in the step 6) at the booting stage, identifying field resistance in different rice blast disease gardens, integrating the rice blast and disease garden resistance identification results, and comprehensively evaluating the rice blast resistance of the bred restorer line and the hybridization combination configured by taking the indica rice restorer line seeds as the male parent; harvesting the rice of the hybrid combination, and detecting the quality of the rice.
5. A breeding method of new combination of high-quality rice blast-resistant high-yield hybrid indica rice is characterized in that: on the basis of a high-quality rice blast resistance strong-dominance restoring line bred according to the breeding method of claim 1, different sterile lines are used as female parents to configure a hybrid combination, the rice blast strains used in the step 6) are inoculated in the booting stage of the hybrid progeny under the premise of ensuring that the yield is increased compared with the yield of a hybrid indica regional trial control variety II-Yo 084 in Jiangsu province or a indica control variety Fengliou 4 in a national regional trial, the panicle blast resistance of the hybrid combination is identified, and the rice blast resistance of the configured hybrid combination is comprehensively evaluated by combining the resistance identification results of different rice blast disease gardens; and (3) harvesting rice of hybrid progeny of all the hybrid combinations, detecting the rice quality of all the hybrid combinations according to the national standard of high-quality rice, and finally obtaining the high-quality rice blast resistant high-yield hybrid indica rice new combination.
6. The breeding method according to claim 5, wherein the different disease nursery is selected from 4 disease nursery of rice blast in Hangzhou, Jiangxi Jinggang mountain, Anhui Jinzhai and Enshi in Hubei province.
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