CN104521758A - New breeding method for resisting rice blast through indica-japonica hybrid gene introgression - Google Patents
New breeding method for resisting rice blast through indica-japonica hybrid gene introgression Download PDFInfo
- Publication number
- CN104521758A CN104521758A CN201510000782.3A CN201510000782A CN104521758A CN 104521758 A CN104521758 A CN 104521758A CN 201510000782 A CN201510000782 A CN 201510000782A CN 104521758 A CN104521758 A CN 104521758A
- Authority
- CN
- China
- Prior art keywords
- rice
- japonica
- xian
- medium
- blast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a new breeding method for indica-japonica hybrid gene introgression rice resistant to rice blast. The new breeding method comprises the following steps of 1 selecting a japonica rice variety/series to be improved and an indica rice resistant source resistant to rice blast, 2 carrying out course identification of toxin resistant concentration on the selected japonica rice variety/series, 3 carrying out cross breeding to obtain three-way cross F1 hybrid cultivar, 4 carrying out indica removing culture medium anther culture on the three-way cross F1 hybrid cultivar, 5 planting anther cultured seedlings in fields, harvesting single plants to obtain a plurality of japonica rice anther cultured families, further sowing the different japonica rice anther cultured families, and obtaining a japonica rice new strain with good agronomic characters and improved rice blast resistance by using the parents of the japonica rice variety/series to be improved as comparison. According to the new breeding method for indica-japonica hybrid gene introgression rice resistant to rice blast, rice blast resistant genes of indica rice can be specifically and efficiently infiltrated into the japonica rice, rice blast resistant seedling can be completed in about three years, the conventional seedling period of indica-japonica hybrid rice resistant to rice blast is shortened obviously, and the method has large breeding application value.
Description
Technical field
The present invention relates to technical field of agriculture science, refer in particular to a kind of specific and efficiently long-grained nonglutinous rice rice blast resistance gene is infiltrated the rice breeding method of japonica rice, specifically gene introgression blast resisting breeding new method handed over by a kind of Xian round-grained rice.
Background technology
Paddy rice is one of most important cereal crops in the whole world, and its area under cultivation and gross yield are only second to wheat.Due in the population of Asia 2,000,000,000 4/5 and Africa and nearly 1,000,000,000 populations in Latin America in 1/3 take all rice as main food source, so Rice Production is particularly outstanding to the importance of developing country.China is the big country that paddy is produced.According to the statistics over nearly 10 years, annual produce that paddy accounts for that world's paddy always produces 36.9%, rank first in the world; Rice growing area accounts for 22.8% of the total sowing area in the world, is only second to India; The average yield per mu of paddy is about 380 kilograms, higher than world average yield per mu, comes out at the top in main Chan Dao state.In recent years, along with the raising of China's industrial expansion and people's living standard, the demand of rice is continued to increase, and along with the outburst of rice pest, population sustainable growth, arid aggravation, rural labour are deficient and the impact of the key element such as environmental pollution, China's Rice Production does not catch up with the increase of demand just day by day, the degree of self-sufficiency of China's grain is declined year by year, and Rice Cropping and grain safety problem are faced with stern challenge.
The developing history of crop breeding shows, all breeding tactics have breakthrough, and will bring a qualitative leap to agricultural production.Since half a century, rice breeding experienced by 2 great revolutions, is the breeding wheat for semidwarfness occurring in the sixties in 20th century for the first time, and current revolution becomes modern dwarfted varieties traditional long-stalked variety, makes output increased 20% ~ 30%; Second time is the heterosis utilization occurring in 20 century 70s, and three series mating large-area applications are in production, and rice yield improves 20% than the sixties again.The cultivation success of hybrid rice, improves China paddy rice significantly and always produces.Twice breeding revolution makes the Rice Production in the world obtain large development, plays very important effect for supporting growing world population.
Indica rice breaks up and significant difference deeply, and make them differ from one another in output, quality and resistance etc., no matter its Hybrids F1 all has powerful hybrid vigour at nutrition organs or on yield traits.Yuan Longping proposes the imagination of subspecies indica and japonica hybrid use of advantage first, think and directly utilize inter-subspecific heterosis to be to be hopeful most during output breaks through and can compared with the approach obtaining effect in short-term, propose application wide compatibility gene and phto-temperature reaction and realize the strategy flexibility that inter-subspecific heterosis directly utilizes.Rice Cross Breeding Between Indica And Japonica will break the boundary of breeding in subspecies, and in subspecies, subspecies breeding is moved towards in breeding.Indica-japonica hybrid is sub-distant hybridization, and filial generation easily produces huge variation and heterobeltiosis, contains huge genetic potential.Indica-japonica hybridization conventional breeding and subspecies indica and japonica hybrid use of advantage have become important means and the method for the research of current Rice Super-yield Breeding.The breeding practice of China also proves, utilizes Indica-japonica hybridization to be create the effective method of novel strain type excellent germplasm.Current Xian round-grained rice hands over the kind be bred as to have the short round-grained rice 23 in Zhejiang, T209 and city spy 232 etc., No. 5, the distant round-grained rice in Liaoning, distant round-grained rice 326, Shen Nong 1033, and the Zijin round-grained rice in Jiangsu, makes system in Pekinese, and Hubei Province in Hubei educates late No. 5 etc.These kinds (being) show the good characteristic of high yield, disease-resistant and high-quality, but some Hybrids of Indica and Japonica Posterity phenotypes go out some shortcomings, as the not blast resisting of " thousandweight wave ", " southern round-grained rice 35 " be easy to Spike sprouting etc.
Abroad indica-japonica hybrid traditional breeding method is also paid close attention.From the middle and later periods sixties, the indica-japonica hybrid conventional breeding of Korea S achieves remarkable achievement, and be bred as the high yield Dwarf-lines systems such as unification, water are former, close sun, these kinds are generally than traditional round-grained rice type kind volume increase 20% ~ 40%.Japan introduces the Indica Rice kind on the ground such as China and Korea S, hybridize with local japonica rice variety, increase the floret bears of hybrid generation, improve resistance and the yield stability of filial generation simultaneously, successively 52 new lines likely and 5 new varieties are bred as, as China 91, northern land 125, northern land 142, the Northeast 146, Xing Feng, Qiu Li etc., than check variety volume increase 20% ~ 36%.Breeding man of Japan is thought, the index that will realize volume increase 50% in the phase III must rely on hybrid rice, and particularly after paddy rice wide compatibility gene theory proposes, the main subspecies indica and japonica hybrid use of advantage approach that relies on reaches.
But, because Xian, round-grained rice belong to two subspecies, although Fl nutritional advantages is very strong.But generally all there is part reproductive disorder, the plant height of subspecies indica and japonica hybrid F1 generation is higher, and breeding time is super close late-maturing, and the abortion of pollen part, the problem such as ripening rate is on the low side, be the Three Obstacles that Xian round-grained rice friendship breeding faces, seriously limit the direct utilization of this advantage on producing.No matter Xian round-grained rice is conventional rice variety seed selection or Breeding for restoration lines if being handed over and utilized, be substantially to be based upon on random combo and a large amount of basis selected, and needs to expend years and the huge amount of labour.
Research finds, typical Xian Xian hands over and round-grained rice round-grained rice is transferred in gene exchange few, and Fl advantage is weak, and excessive Xian round-grained rice gene exchange is also unfavorable for that F1 is nourishing and growing and the coordination on reproductive growth, be difficult to equally show yield heterosis, this just relates to the degree problem of Xian round-grained rice gene introgression.The breeding fact shows, Xian round-grained rice gene introgression has played important function in Breeding on Super Hybrid Rice, and by Molecular Detection, the parent of a lot of super hybridized rice is all obtained by Xian round-grained rice gene introgression.But gene introgression is in Super rice breeding, and be realized by the hybridization in repeatedly generation, the screening that backcrosses, breeding cycle is long, and workload is huge.
Rice blast (Blast Fungus), Ge Dao district of the world evenly occurs, and is that rice district, the whole world endangers one of the most serious rice disease, has another name called rice blast, be commonly called as and burn pest, hang a pest, pinch neck pest etc.This disease all can work the mischief to paddy rice throughout the year, its harm, throughout each position of paddy rice, has seedling rice blast, leaf pest, pulvinus pest, joint rice blast, panicle blast, branch stalk pest and grain pest etc., generally causes the paddy rice underproduction 10% ~ 20%, the underproduction 40% ~ 50% time serious, even No kernels or seeds are gathered, as in a year of scarcity.For China, since the nineties in 20th century, this disease year occurring area all at 3,800,000 hm
2above, year loss paddy reaches several hundred million kilograms.In recent years, rice blast occurs increasing trend year by year, and local great outburst often occurs, and brings serious threat to paddy rice production safety.How effectively preventing rice blast is numerous agro-technicians and peasant's urgent problem.Integrated control technique is taked to prevent the generation of rice blast in present agricultural production, Main Means takes some preventive measures, antibiotic and sterilizing means and applying pesticides is adopted to carry out pharmacotherapy as selected in disease-resistant variety, cultivation, and set up rice blast forecast system, long-term production practices prove, the Breeding and application of Rice Resistance To Rice Blast kind is the most economical effective measures of control rice blast.
Mainly in blast resisting breeding in the early stage adopting traditional disease resistant and breeding method to carry out breeding, introducing a fine variety as utilized, selection and use, the conventional breeding approach such as crossbreeding select a collection ofly has vertical resistance and the good excellent strain of horizontal resistance.But because large-area unification uses certain resistance gene, cause the M8003 line effect to rice blast colony, the complexity of rice blast pathogen biological strain heredity and pathogenic diversity make the composition of the virulence gene of rice blast pathogen colony change, often namely the resistant variety of seed selection produces the dominant races that can infect this kind after 3 ~ 5 years in popularization, finally causes the resistant lose of new varieties.Conventional breeding elapsed time is long, and especially along with the increase of rice blast pathogen mutation frequency, the speed of a new varieties incubation has not caught up with the mutating speed of rice blast pathogen.
Break up deeply and significant difference at indica rice, them are made to differ from one another in output, quality and resistance etc., one of them is at the Resistant Difference highly significant of rice blast pathogen, the multipair rice blast of rice variety has higher resistance level, and the resistance of japonica rice to rice blast is poor, show to exist containing the unexistent blast resistant gene of a large amount of japonica rice in long-grained nonglutinous rice genome.Cultivate blast resisting japonica rice variety if shifted by accurate means efficiently by the blast resistant gene in long-grained nonglutinous rice into japonica rice genome, production has high value.
Along with the development of biotechnology, many new breeding techniques have been there is again except conventional cross-breeding, this give rice anti-rice blast breeding new way, one utilizes rice blast culture fluid to extract Raw toxin as Selective Pressure, carry out screening mutant on a cellular level, and be combined with traditional breeding method and cultivate new disease-resistant varieties, but low by the frequency of Raw toxin induced mutations, screening amount is large; The molecular breeding technology that the second is exactly is representative with transgenic technology and molecular marker assisted selection breeding technique.The appearance of molecular breeding technology, breeding difficulty is handed over to be a kind of feasible solution originally to rice anti-rice blast breeding and Xian round-grained rice, at present, transgenic breeding and molecular marker assisted selection equimolecular technology Breeding are the new roads of current agricultural development, are known as the appearance of the third time agriculture green revolution by scientific circles.But, specific excellent main breed is carried out to the transgenic breeding of blast resistant gene and other excellent genes, originally accurately can realize the improvement that Xian round-grained rice is handed over, but be resisted due to current transgenic breeding, and first molecular marker assisted selection breeding needs gene location, the hybridization of land for growing field crops many generations is needed to backcross equally, the problem that Xian round-grained rice is handed over can not be solved, in addition, molecular engineering breeding, all exist costly, problem that technical threshold is high, to be difficult to grasp by general breeder.
Summary of the invention
The Hybrids F1 plant height that the object of the invention is to hand over conventional breeding to face for existing Xian round-grained rice is higher, breeding time super close late-maturing, obstacle that pollen part abortion ripening rate is on the low side, provide a kind of by specific for long-grained nonglutinous rice rice blast resistance gene and infiltrate japonica rice efficiently thus the Xian round-grained rice friendship gene introgression blast resisting breeding new method of improvement japonica rice variety.
The object of the invention is to solve by the following technical programs:
Gene introgression blast resisting breeding new method handed over by a kind of Xian round-grained rice, it is characterized in that: the step of this breeding new method is as follows:
(1) choose and wait to improve japonica rice variety/be source anti-with blast resisting long-grained nonglutinous rice: choose the good japonica rice variety of economical character/be and to the japonica rice variety chosen/be the Fiber differentiation carrying out arranging Xian medium, final selected induction of anther callus rate higher than 25% japonica rice variety/be as treating improvement japonica rice variety/be, choose the good and rice variety containing rice blast high resistance gene of economical character/be as the anti-source of blast resisting long-grained nonglutinous rice;
(2) improvement japonica rice variety/be the qualification carrying out Raw toxin patience concentration is treated to what choose: the Extracted toxin liquid adding certain gradient concentration respectively in differential medium, differentiation cultivation is carried out to the anther callus waiting to improve japonica rice variety/be selected in step (1), and selects the Extracted toxin liquid of suitable screening pressure level concentration as japonica rice anther culture Raw toxin Selective Pressure;
(3) crossbreeding obtains three and hands over F1 crossbreed: the anti-source of blast resisting long-grained nonglutinous rice of screen field planting and Wide compability material Xian round-grained rice are built bridge and obtained Hybrid F1, the flower pesticide choosing Hybrid F1 again with wait to improve japonica rice variety/be and hybridize and obtain crossbreed three and hand over F1;
(4) F1 crossbreed is handed over to carry out row Xian medium anther culture to three: three hand over the positive season sowing of F1 crossbreed, choose healthy and strong main Honoka medicine and carry out row Xian medium flower pesticide Fiber differentiation, obtain anther callus, and the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium that with the addition of Selective Pressure concentration Raw toxin, temperature 27 ~ 29 DEG C, cultivate under the condition of the dark 10h of light 14h/, obtain flower training seedling, treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, growing to until bloom control to transplant to Turnover Box strong sprout after 8cm, land for growing field crops is transplanted into after 10 ~ 15 days,
(5) bloom control individual plant sowing obtains flower training family, the screening of further economical character, rice blast resistance obtains the Keng rice lines of improvement: field planting bloom control, the japonica rice training family that individual plant sowing obtains rice blast resistance improvement is some, the further field seeding of training family of not suiting, to treat that improvement japonica rice variety/be parent is for contrast, screening obtains the Keng rice lines that the good and rice blast resistance of agronomic shape obtains improveing.
The japonica rice variety that economical character in described step (1) is good/be refers to that output is high, good grain quality, rice blast resistance need to be improved and plant height should not too high, breeding time is precocious or in ripe japonica rice variety/be; The anti-source of blast resisting long-grained nonglutinous rice refers to that output is high, the rice variety of good grain quality, high resistant to rice blast/be.
The process that the japonica rice variety that the economical character chosen in described step (1) is good/be carries out the Fiber differentiation arranging Xian medium is as follows:
(1.1) to draw materials and pretreatment: by japonica rice variety good for the economical character chosen/be land for growing field crops conventional cultivation, get pulvinus apart from be 5 ~ 7cm, fringe look be pistac, flower pesticide is faint yellow and length be about clever shell 1/3 ~ 1/2, the main fringe of stalwartness that do not split of booting is for for trying material, retain 2 ~ 3 blades, behind 75% alcohol wipe surface, wrap up and overcoat plastic sack with wet towel, Cold pretreatment 3 days at putting 4 DEG C;
(1.2) inoculate: after pretreatment, rice bud containing main fringe is cut, then this rice bud is soaked in the alcohol of 75% and processes 3 ~ 4min, then this rice bud is shifted and peel off main fringe in superclean bench, strip uniform ramuscule stalk sized by small ear further, the following rear concentration of gauze parcel ramuscule stalk of sterilizing is the mercuric chloride sterilizing 10min of 0.1%, rinsed with sterile water 3 ~ 4 times, open gauze to dry, finally cut off flower pesticide top with the scissors of sterilizing, tremble medicine method inoculation flower pesticide in row Xian medium;
(1.3) Fiber differentiation: dark surrounds evoked callus row Xian medium being placed in 24 ~ 26 DEG C, white-flaxen unformed cell mass is there is at the edge of flower pesticide after 30 ~ 40 days, and add up induction of anther callus rate afterwards flower pesticide inoculated and cultured 50 days, selected induction of anther callus rate higher than the good japonica rice variety of the economical character of 25%/be as treating improvement japonica rice variety/be.
Rice bud in described step (1.2) refers in the region of rice bud top pillow distance for internode top 0.5cm under 7cm to fringe the part of cutting material and obtaining, and the upper flower pesticide quantity of the ramuscule stripped stalk is 15 ~ 25.
Row Xian culture medium prescription in described step (1.2) is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, and the pH value of row Xian medium is 5.8.
Raw toxin patience concentration qualification process in described step (2) is as follows:
(2.1) preparation of Raw toxin: mixing Pyricularia oryzae is inoculated in plane expand breeding culture medium expands numerous, be transferred in liquid nutrient medium after growing mycelium and produce spore, sterile gauze elimination bacterium is used after shaken cultivation more than 20 days under the condition of 25 DEG C, centrifugal 20min under the rotating speed of 1000r/min, get supernatant 115 ~ 125 DEG C of high-temperature sterilization 20min, the bacteria-free filtrate that obtains is Crude Toxin Produced by Pyricularia Oryzae;
(2.2) treat that improvement japonica rice variety/be carries out the qualification of Raw toxin endurance concentration to what choose: the Extracted toxin liquid adding certain gradient concentration respectively in differential medium, then the anther callus waiting to improve japonica rice variety/be Fiber differentiation selected in step (1) is shifted into differential medium, Fiber differentiation added up phenylacetic acid after 30 ~ 40 days, and selected the Extracted toxin liquid of suitable screening pressure level concentration to hand over F1 crossbreed anther culture Raw toxin Selective Pressure concentration as three.
Suitable screening pressure level concentration in described step (2.2) refers to the selected Raw toxin concentration of anther callus when being 0.8% ~ 1.2% containing the differentiation rate in the differential medium of Raw toxin until improvement japonica rice variety/be Fiber differentiation.
Plane in described step (2.1) expands breeding culture medium formula: yeast extract 2.5g/L+ soluble starch 10g/L+ agar powder 16g/L, and pH value is 6.0; Liquid culture based formulas is KH
2pO
40.5g/L+K
2hPO
40.5g/L+MgSO
40.5g/L+ glucose 25g/L+ dusty yeast 6g/L, pH value nature; Differential medium formula in described step (2.2) is: MS minimal medium+2mg/L6-BA+0.2mg/LNAA+ gradient concentration Extracted toxin liquid, the pH value of differential medium is 6.0.
In described step (4) three hand over the induction of anther callus rate of F1 crossbreed lower than 3% time, F1 crossbreed and japonica rice parent need be handed over to backcross by three and obtain BC1F1 crossbreed, the positive season sowing of BC1F1 crossbreed, choose healthy and strong main fringe and re-start row Xian medium flower pesticide Fiber differentiation, until obtain the anther callus that inductivity is not less than 3%, the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium adding Raw toxin Selective Pressure, temperature 27 ~ 29 DEG C, cultivate under the condition of the dark 10h of light 14h/, obtain flower training seedling, treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, growing to until bloom control to transplant to Turnover Box strong sprout after 8cm, land for growing field crops is transplanted into after 10 ~ 15 days.
Row Xian culture medium prescription in described step (4) is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, and the pH value of row Xian medium is 5.8; Differential medium formula is: the Extracted toxin liquid of MS mineral salt+MS organic matter+suitable screening pressure level concentration of sucrose 3%+ agar 0.7%+KT2.0mg/L+NAA0.5mg/L+ protein hydrolysate 1g/L+, and the pH value of differential medium is 5.8; Prescription of rooting medium is: a large amount of+Ms trace+Ms organic matter+sucrose 3%+ agar 0.8% of 1/2Ms, the pH value of root media is 5.8.
The present invention has the following advantages compared to existing technology:
Breeding new method of the present invention, compared with conventional breeding methods, by specific for long-grained nonglutinous rice blast resistant gene and infiltrate japonica rice efficiently, can greatly reduce screening operation amount, within about 3 years, can complete breeding, significantly shortens conventional Xian round-grained rice and hands over blast resisting breeding cycle; Compared with molecular engineering breeding, there is again the advantage that operation threshold is relatively low, spend less and breeding cycle to shorten, there is larger Breeding Application and be worth.
Accompanying drawing explanation
Accompanying drawing 1 is breeding new method and technique route map of the present invention.
Embodiment
Below in conjunction with accompanying drawing 1, the present invention is further illustrated with embodiment.
As shown in the Technology Roadmap of Fig. 1: gene introgression blast resisting breeding new method handed over by a kind of Xian round-grained rice, and the step of this breeding new method is as follows:
(1) choose and treat improvement japonica rice variety/be source anti-with blast resisting long-grained nonglutinous rice:
Choose the good japonica rice variety of economical character/be, because Xian round-grained rice is handed over, F-1 hybrids has that plant height is higher usually, breeding time super close late-maturing feature, therefore the good japonica rice variety of this economical character/be refers to that output is high, good grain quality, rice blast resistance need improvement and plant height should not too high, breeding time is precocious or in ripe japonica rice variety/be, then the Fiber differentiation arranging Xian medium is carried out to the japonica rice variety chosen/be, whether flower pesticide evoked callus is carried out in the row of being applicable to Xian medium to check this japonica rice variety/be, the process of Fiber differentiation is as follows: (1.1) draw materials and pretreatment: by japonica rice variety good for the economical character chosen/be land for growing field crops conventional cultivation, getting pulvinus distance is 5 ~ 7cm, fringe look is pistac, flower pesticide is faint yellow and length is about 1/3 ~ 1/2 of clever shell, the main fringe of stalwartness that booting is not split is for supplying examination material, retain 2 ~ 3 blades, behind 75% alcohol wipe surface, wrap up and overcoat plastic sack with wet towel, Cold pretreatment 3 days at putting 4 DEG C, (1.2) inoculate: after pretreatment, rice bud containing main fringe is cut, rice bud refers in the region of rice bud top pillow distance for internode top 0.5cm under 7cm to fringe the part of cutting material and obtaining, then this rice bud is soaked in the alcohol of 75% and processes 3 ~ 4min, then this rice bud is shifted and peel off main fringe in superclean bench, strip uniform ramuscule stalk sized by small ear further, the upper flower pesticide restricted number of each ramuscule stalk is 15 ~ 25, following sterilized gauze parcel ramuscule stalk rear concentration be 0.1% mercuric chloride sterilizing be not less than 10min, rinsed with sterile water 3 ~ 4 times, open gauze to dry, finally cut off flower pesticide top with the scissors of sterilizing, tremble medicine method inoculation flower pesticide in row Xian medium, row Xian culture medium prescription is: N6 macroelement+MS trace element+MS organic matter+2mg/L2, 4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, the pH value of this row Xian medium is 5.8, (1.3) Fiber differentiation: dark surrounds evoked callus row Xian medium being placed in 24 ~ 26 DEG C, white-flaxen unformed cell mass is there is at the edge of flower pesticide after 30 ~ 40 days, and add up induction of anther callus rate afterwards flower pesticide inoculated and cultured 50 days, selected induction of anther callus rate higher than the good japonica rice variety of the economical character of 25%/be as treating improvement japonica rice variety/be.Choose the good and rice variety containing rice blast high resistance gene of economical character/be as the anti-source of blast resisting long-grained nonglutinous rice simultaneously, the anti-source of blast resisting long-grained nonglutinous rice refers to that output is high, the rice variety of good grain quality, high resistant to rice blast/be, guarantees that rice blast high resistance gene is contained in the anti-source of this long-grained nonglutinous rice.
(2) improvement japonica rice variety/be the qualification carrying out Raw toxin patience concentration is treated to what choose:
Raw toxin patience concentration qualification process is as follows: the preparation of (2.1) Raw toxin: mixing Pyricularia oryzae is inoculated in plane expand breeding culture medium expands numerous, plane expands breeding culture medium formula: yeast extract 2.5g/L+ soluble starch 10g/L+ agar powder 16g/L and pH value is 6.0, be transferred in liquid nutrient medium after growing mycelium and produce spore, liquid culture based formulas is KH
2pO
40.5g/L+K
2hPO
40.5g/L+MgSO
40.5g/L+ glucose 25g/L+ dusty yeast 6g/L and pH value nature, sterile gauze elimination bacterium is used after shaken cultivation more than 20 days under the condition of 25 DEG C, centrifugal 20min under the rotating speed of 1000r/min, get supernatant 115 ~ 125 DEG C of high-temperature sterilization 20min, the bacteria-free filtrate that obtains is Crude Toxin Produced by Pyricularia Oryzae, (2.2) treat that improvement japonica rice variety/be carries out the qualification of Raw toxin endurance concentration to what choose: the Extracted toxin liquid adding certain gradient concentration respectively in differential medium, then the anther callus waiting to improve japonica rice variety/be Fiber differentiation selected in step (1) is shifted into differential medium, differential medium formula is: MS minimal medium+2mg/L6-BA+0.2mg/LNAA+ gradient concentration Extracted toxin liquid and the pH value of differential medium is 6.0, Fiber differentiation added up phenylacetic acid after 30 ~ 40 days, and select the Extracted toxin liquid reaching suitable screening pressure level concentration to hand over F1 crossbreed anther culture Raw toxin Selective Pressure concentration as three, wherein suitable screening pressure level concentration refers to the selected Raw toxin concentration of anther callus when being 0.8% ~ 1.2% containing the differentiation rate in the differential medium of Raw toxin until improvement japonica rice variety/be Fiber differentiation.
(3) crossbreeding obtains three and hands over F1 crossbreed:
The anti-source of blast resisting long-grained nonglutinous rice of screen field planting and Wide compability material Xian round-grained rice are built bridge and are obtained Hybrid F1, the flower pesticide choosing Hybrid F1 again with wait to improve japonica rice variety/be and hybridize and obtain crossbreed three and hand over F1.
(4) F1 crossbreed is handed over to carry out row Xian medium anther culture to three:
Three hand over the positive season sowing of F1 crossbreed, choose healthy and strong main Honoka medicine and carry out row Xian medium flower pesticide Fiber differentiation acquisition anther callus, row Xian culture medium prescription is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, the pH value of row Xian medium is 5.8, when three hand over the induction of anther callus rate of F1 crossbreed lower than 3% time, F1 crossbreed and japonica rice parent need be handed over to backcross by three and obtain BC1F1 crossbreed, the positive season sowing of BC1F1 crossbreed, choose healthy and strong main fringe and re-start row Xian medium flower pesticide Fiber differentiation, until obtain the anther callus that inductivity is not less than 3%, the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium that with the addition of Selective Pressure concentration Raw toxin, differential medium formula is: the Extracted toxin liquid of the gradient concentration of MS mineral salt+MS organic matter+sucrose 3%+ agar 0.7%+KT2.0mg/L+NAA0.5mg/L+ protein hydrolysate 1g/L+ screening pressure level and the pH value of differential medium is 5.8, temperature 27 ~ 29 DEG C, cultivate under the condition of the dark 10h of light 14h/, obtain flower training seedling, treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, prescription of rooting medium is: a large amount of+Ms trace+Ms organic matter+sucrose 3%+ agar 0.8% of 1/2Ms and the pH value of root media is 5.8, grow to until bloom control and to transplant after 8cm to Turnover Box strong sprout, after 10 ~ 15 days, be transplanted into land for growing field crops.
(5) bloom control individual plant sowing obtains flower training family, and the screening of further economical character, rice blast resistance obtains the Keng rice lines of improvement:
Field planting bloom control, the japonica rice training family that individual plant sowing obtains rice blast resistance improvement is some, do not suit training family further field seeding, and to treat that improvement japonica rice variety/be parent is for contrast, screening obtains the Keng rice lines that the good and rice blast resistance of agronomic shape obtains improveing.
Embodiment
(1) respectively screen S0412 and ground paddy as Japonica two line hybrid rice parent and the anti-source of long-grained nonglutinous rice rice blast:
Breeding practice for many years finds, breeding trunk strain S0412 has that output is high, the feature of good grain quality, simultaneously, the plant height of S0412 measures for 2 years on average only has 92.8cm, the time of infertility only has 142 days, sense rice blast, and 2011 positive gets S0412 flower pesticide season and be inoculated in and arrange Xian medium and carry out Fiber differentiation, experiment finds that the inductivity of S0412 callus is the same up to 54%(anther culture operating procedure), be applicable to very much the requirement of the Japonica two line hybrid rice parent of this technology path.En Hui58Shi Hubei Province bestows favour, and Academy of Agricultural Sciences of autonomous prefecture adopts extensive 63 to make female parent, Milyang 46 makes the indica type conventional rice that paternal hybrid is bred as, how to, multiple rice blast fungus strain apparent altitude resistances of rice blast lesion, are one of important anti-sources of blast resisting breeding.
(2) S0412 is carried out to the qualification of Raw toxin patience concentration:
The qualification of Raw toxin endurance concentration was carried out to the S0412 chosen in 2011, the Extracted toxin liquid of 5%, 10%, 15%, 20%, 25% is added in differential medium, preparation differential medium, differential medium formula is: MS minimal medium+2mg/L6-BA+0.2mg/LNAA+ gradient concentration Extracted toxin liquid and pH value 6.0, then the anther callus of Fiber differentiation in step (1) is shifted into differential medium, statistics phenylacetic acid.Result is as follows:
| Extracted toxin liquid concentration | 0 | 5% | 10% | 15% | 20% | 25% |
| Anther callus differentiation rate | 38% | 9% | 1.4% | 0.4% | 0.08% | 0 |
S0412 further south is numerous, and obtain flower pesticide and carry out Fiber differentiation, and carry out the optimization of Raw toxin patience concentration further, result is as follows:
| Extracted toxin liquid concentration | 10% | 11% | 12% | 13% | 14% | 15% |
| Anther callus differentiation rate | 1.5% | 1.3% | 1.0 % | 0.7% | 0.5% | 0.3% |
Therefore, the Extracted toxin liquid concentration of 12% is decided to be three friendship F1 crossbreed anther culture Raw toxin Selective Pressure by us.
(3) crossbreeding obtains three and hands over F1 crossbreed:
Grace in 2011 extensive 58 and Wide compability japonica rice 02428 of short stem hybridizes builds bridge, and gathers in the crops Hybrid F1, and Hainan in 2012 is chosen F1 flower pesticide and hybridized with S0412 and obtain crossbreed three and hand over F1.
(4) F1 crossbreed is handed over to carry out row Xian medium anther culture to three:
Positive season in 2012, land for growing field crops general planting three handed over F1 crossbreed, choose healthy and strong main Honoka medicine to be inoculated in row's Xian medium and to carry out flower pesticide Fiber differentiation and obtain anther callus, row Xian culture medium prescription is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, pH value 5.8; And the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium that with the addition of 12% Extracted toxin liquid, differential medium formula is: MS mineral salt+MS organic matter+sucrose 3%+ agar 0.7%+KT2.0mg/L+NAA0.5mg/L+protein hydrolysate 1g/L+12% Extracted toxin liquid, pH value 6.0, cultivate under the condition of temperature 27 ~ 29 DEG C, the dark 10h of light 14h/, obtain 113 flower training seedling; Treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, prescription of rooting medium is: a large amount of+Ms trace+Ms organic matter+sucrose 3%+ agar 0.8% of 1/2Ms and the pH value of root media is 5.8, grow to until bloom control and to transplant after 8cm to Turnover Box strong sprout, within 2013, be transplanted into greenhouse.
(5) flower training family field planting, the screening of economical character, rice blast resistance obtains 2 excellent Keng rice lines of rice blast resistance improvement:
Be transplanted to the bloom control individual plant sowing in greenhouse, obtain 113 flower training familys, positive season in 2014 further field seeding 113 flower training familys take S0412 as contrast, therefrom screen the Keng rice lines that the good and rice blast resistance of acquisition 2 agronomic shapes obtains improvement.
Breeding new method of the present invention, compared with conventional breeding methods, by special for long-grained nonglutinous rice blast resistant gene and infiltrate japonica rice efficiently, can greatly reduce screening operation amount, within about 3 years, can complete breeding, significantly shortens conventional Xian round-grained rice and hands over blast resisting breeding cycle; Compared with molecular engineering breeding, there is again the advantage that operation threshold is relatively low, spend less and breeding cycle to shorten, there is larger Breeding Application and be worth.
Above embodiment is only and technological thought of the present invention is described, can not limit protection scope of the present invention with this, every technological thought proposed according to the present invention, and any change that technical scheme basis is done, all falls within scope; The technology that the present invention does not relate to all is realized by prior art.
Claims (10)
1. a gene introgression blast resisting breeding new method handed over by Xian round-grained rice, it is characterized in that: the step of this breeding new method is as follows:
(1) choose and wait to improve japonica rice variety/be source anti-with blast resisting long-grained nonglutinous rice: choose the good japonica rice variety of economical character/be and to the japonica rice variety chosen/be the Fiber differentiation carrying out arranging Xian medium, final selected induction of anther callus rate higher than 25% japonica rice variety/be as treating improvement japonica rice variety/be, choose the good and rice variety containing rice blast high resistance gene of economical character/be as the anti-source of blast resisting long-grained nonglutinous rice;
(2) improvement japonica rice variety/be the qualification carrying out Raw toxin patience concentration is treated to what choose: the Extracted toxin liquid adding certain gradient concentration respectively in differential medium, differentiation cultivation is carried out to the anther callus waiting to improve japonica rice variety/be selected in step (1), and selects the Extracted toxin liquid of suitable screening pressure level concentration as japonica rice anther culture Raw toxin Selective Pressure;
(3) crossbreeding obtains three and hands over F1 crossbreed: the anti-source of blast resisting long-grained nonglutinous rice of screen field planting and Wide compability material Xian round-grained rice are built bridge and obtained Hybrid F1, the flower pesticide choosing Hybrid F1 again with wait to improve japonica rice variety/be and hybridize and obtain crossbreed three and hand over F1;
(4) F1 crossbreed is handed over to carry out row Xian medium anther culture to three: three hand over the positive season sowing of F1 crossbreed, choose healthy and strong main Honoka medicine and carry out row Xian medium flower pesticide Fiber differentiation, obtain anther callus, and the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium that with the addition of Selective Pressure concentration Raw toxin, temperature 27 ~ 29 DEG C, cultivate under the condition of the dark 10h of light 14h/, obtain flower training seedling, treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, growing to until bloom control to transplant to Turnover Box strong sprout after 8cm, land for growing field crops is transplanted into after 10 ~ 15 days,
(5) bloom control individual plant sowing obtains flower training family, the screening of further economical character, rice blast resistance obtains the Keng rice lines of improvement: field planting bloom control, the japonica rice training family that individual plant sowing obtains rice blast resistance improvement is some, the further field seeding of training family of not suiting, to treat that improvement japonica rice variety/be parent is for contrast, screening obtains the Keng rice lines that the good and rice blast resistance of agronomic shape obtains improveing.
2. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 1, it is characterized in that: the japonica rice variety that the economical character in described step (1) is good/be refers to that output is high, good grain quality, rice blast resistance need to be improved and plant height should not too high, breeding time is precocious or in ripe japonica rice variety/be; The anti-source of blast resisting long-grained nonglutinous rice refers to that output is high, the rice variety of good grain quality, high resistant to rice blast/be.
3. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 1, it is characterized in that: the process that the japonica rice variety that the economical character chosen in described step (1) is good/be carries out the Fiber differentiation arranging Xian medium is as follows:
(1.1) to draw materials and pretreatment: by japonica rice variety good for the economical character chosen/be land for growing field crops conventional cultivation, get pulvinus apart from be 5 ~ 7cm, fringe look be pistac, flower pesticide is faint yellow and length be about clever shell 1/3 ~ 1/2, the main fringe of stalwartness that do not split of booting is for for trying material, retain 2 ~ 3 blades, behind 75% alcohol wipe surface, wrap up and overcoat plastic sack with wet towel, Cold pretreatment 3 days at putting 4 DEG C;
(1.2) inoculate: after pretreatment, rice bud containing main fringe is cut, then this rice bud is soaked in the alcohol of 75% and processes 3 ~ 4min, then this rice bud is shifted and peel off main fringe in superclean bench, strip uniform ramuscule stalk sized by small ear further, the following rear concentration of gauze parcel ramuscule stalk of sterilizing is the mercuric chloride sterilizing 10min of 0.1%, rinsed with sterile water 3 ~ 4 times, open gauze to dry, finally cut off flower pesticide top with the scissors of sterilizing, tremble medicine method inoculation flower pesticide in row Xian medium;
(1.3) Fiber differentiation: dark surrounds evoked callus row Xian medium being placed in 24 ~ 26 DEG C, white-flaxen unformed cell mass is there is at the edge of flower pesticide after 30 ~ 40 days, and add up induction of anther callus rate afterwards flower pesticide inoculated and cultured 50 days, selected induction of anther callus rate higher than the good japonica rice variety of the economical character of 25%/be as treating improvement japonica rice variety/be.
4. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 3, it is characterized in that: the rice bud in described step (1.2) refers in the region of rice bud top pillow distance for internode top 0.5cm under 7cm to fringe the part of cutting material and obtaining, and the upper flower pesticide quantity of the ramuscule stripped stalk is 15 ~ 25.
5. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 3, it is characterized in that: the row Xian culture medium prescription in described step (1.2) is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, the pH value of row Xian medium is 5.8.
6. gene introgression blast resisting breeding new method handed over by the Xian round-grained rice according to claim 1 or 3, it is characterized in that: the Raw toxin patience concentration qualification process in described step (2) is as follows:
(2.1) preparation of Raw toxin: mixing Pyricularia oryzae is inoculated in plane expand breeding culture medium expands numerous, be transferred in liquid nutrient medium after growing mycelium and produce spore, sterile gauze elimination bacterium is used after shaken cultivation more than 20 days under the condition of 25 DEG C, centrifugal 20min under the rotating speed of 1000r/min, get supernatant 115 ~ 125 DEG C of high-temperature sterilization 20min, the bacteria-free filtrate that obtains is Crude Toxin Produced by Pyricularia Oryzae;
(2.2) treat that improvement japonica rice variety/be carries out the qualification of Raw toxin endurance concentration to what choose: the Extracted toxin liquid adding certain gradient concentration respectively in differential medium, then the anther callus waiting to improve japonica rice variety/be Fiber differentiation selected in step (1) is shifted into differential medium, Fiber differentiation added up phenylacetic acid after 30 ~ 40 days, and selected the Extracted toxin liquid of suitable screening pressure level concentration to hand over F1 crossbreed anther culture Raw toxin Selective Pressure concentration as three.
7. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 6, it is characterized in that: the suitable screening pressure level concentration in described step (2.2) refers to the selected Raw toxin concentration of anther callus when being 0.8% ~ 1.2% containing the differentiation rate in the differential medium of Raw toxin until improvement japonica rice variety/be Fiber differentiation.
8. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 1, it is characterized in that: the plane in described step (2.1) expands breeding culture medium formula and is: yeast extract 2.5g/L+ soluble starch 10g/L+ agar powder 16g/L, and pH value is 6.0; Liquid culture based formulas is KH
2pO
40.5g/L+K
2hPO
40.5g/L+MgSO
40.5g/L+ glucose 25g/L+ dusty yeast 6g/L, pH value nature; Differential medium formula in described step (2.2) is: MS minimal medium+2mg/L6-BA+0.2mg/LNAA+ gradient concentration Extracted toxin liquid, the pH value of differential medium is 6.0.
9. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 1, it is characterized in that: three in described step (4) hand over the induction of anther callus rate of F1 crossbreed lower than 3% time, F1 crossbreed and japonica rice parent need be handed over to backcross by three and obtain BC1F1 crossbreed, the positive season sowing of BC1F1 crossbreed, choose healthy and strong main fringe and re-start row Xian medium flower pesticide Fiber differentiation, until obtain the anther callus that inductivity is not less than 3%, the anther callus selecting diameter 0.4 ~ 0.8cm is inoculated in the differential medium adding Raw toxin Selective Pressure, temperature 27 ~ 29 DEG C, cultivate under the condition of the dark 10h of light 14h/, obtain flower training seedling, treat that bloom control is transferred in root media and carried out squamous subculture after growing to 2cm, growing to until bloom control to transplant to Turnover Box strong sprout after 8cm, land for growing field crops is transplanted into after 10 ~ 15 days.
10. gene introgression blast resisting breeding new method handed over by Xian round-grained rice according to claim 1, it is characterized in that: the row Xian culture medium prescription in described step (4) is: N6 macroelement+MS trace element+MS organic matter+2mg/L2,4-D+0.5mg/LNAA+0.5mg/L protein hydrolysate+5% sucrose+0.8% plant gel, the pH value of row Xian medium is 5.8; Differential medium formula is: the Extracted toxin liquid of MS mineral salt+MS organic matter+suitable screening pressure level concentration of sucrose 3%+ agar 0.7%+KT2.0mg/L+NAA0.5 mg/L+ protein hydrolysate 1g/L+, and the pH value of differential medium is 5.8; Prescription of rooting medium is: a large amount of+Ms trace+Ms organic matter+sucrose 3%+ agar 0.8% of 1/2Ms, the pH value of root media is 5.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510000782.3A CN104521758B (en) | 2015-01-04 | 2015-01-04 | A kind of Xian Jing hand over gene introgression blast resisting breeding new method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510000782.3A CN104521758B (en) | 2015-01-04 | 2015-01-04 | A kind of Xian Jing hand over gene introgression blast resisting breeding new method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104521758A true CN104521758A (en) | 2015-04-22 |
| CN104521758B CN104521758B (en) | 2017-03-29 |
Family
ID=52837542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510000782.3A Active CN104521758B (en) | 2015-01-04 | 2015-01-04 | A kind of Xian Jing hand over gene introgression blast resisting breeding new method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104521758B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069726A (en) * | 2016-06-29 | 2016-11-09 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-tingia dwarfing Oryza sativa L. |
| CN106069725A (en) * | 2016-06-29 | 2016-11-09 | 无锡南理工科技发展有限公司 | A kind of bacterial Footrot resistance Japonica two line hybrid rice method |
| CN109601059A (en) * | 2019-01-21 | 2019-04-12 | 西南科技大学 | A kind of blast resisting biology seed soaking agent and preparation method thereof and application method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044201A (en) * | 1990-03-09 | 1990-08-01 | 江苏省农业科学院粮食作物研究所 | Hybrids of Indica and Japonica rice---inferior excellent No. two breeding method |
| CN1082811A (en) * | 1992-07-23 | 1994-03-02 | 湖南农学院 | Utilize low temperature to castrate the method for preparation indica-japonica hybrid rice |
| CN1159879A (en) * | 1997-01-17 | 1997-09-24 | 四川省农业科学院水稻高粱研究所 | Technique for breeding of hybridized rice |
| CN1366808A (en) * | 2002-02-09 | 2002-09-04 | 四川农业大学 | Method for breeding rice varieties by using early generation stable material |
| CN1887062A (en) * | 2006-07-31 | 2007-01-03 | 中国水稻研究所 | Selective breeding method of hybrid rice of late blooming season long-grained rice restoring line and subspecies |
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
-
2015
- 2015-01-04 CN CN201510000782.3A patent/CN104521758B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044201A (en) * | 1990-03-09 | 1990-08-01 | 江苏省农业科学院粮食作物研究所 | Hybrids of Indica and Japonica rice---inferior excellent No. two breeding method |
| CN1082811A (en) * | 1992-07-23 | 1994-03-02 | 湖南农学院 | Utilize low temperature to castrate the method for preparation indica-japonica hybrid rice |
| CN1159879A (en) * | 1997-01-17 | 1997-09-24 | 四川省农业科学院水稻高粱研究所 | Technique for breeding of hybridized rice |
| CN1366808A (en) * | 2002-02-09 | 2002-09-04 | 四川农业大学 | Method for breeding rice varieties by using early generation stable material |
| CN1887062A (en) * | 2006-07-31 | 2007-01-03 | 中国水稻研究所 | Selective breeding method of hybrid rice of late blooming season long-grained rice restoring line and subspecies |
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
Non-Patent Citations (1)
| Title |
|---|
| 朴钟泽等: ""利用病菌培养液离体筛选水稻抗稻瘟病植株研究"", 《华北农学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069726A (en) * | 2016-06-29 | 2016-11-09 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-tingia dwarfing Oryza sativa L. |
| CN106069725A (en) * | 2016-06-29 | 2016-11-09 | 无锡南理工科技发展有限公司 | A kind of bacterial Footrot resistance Japonica two line hybrid rice method |
| CN109601059A (en) * | 2019-01-21 | 2019-04-12 | 西南科技大学 | A kind of blast resisting biology seed soaking agent and preparation method thereof and application method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104521758B (en) | 2017-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104542256B (en) | A kind of long-grained nonglutinous rice false smut resistance efficiently imports the breeding method of japonica rice | |
| CN104839010B (en) | A kind of selection of paddy rice High quality and diseases resistance is high temperature resistant two-line sterile line | |
| CN104429932B (en) | A kind of Xian round-grained rice hands over the anti-banded sclerotial blight breeding new method of gene introgression | |
| CN103609428B (en) | The method of utilizing the unreduced gamete characteristic of friction standing grain to cultivate corn allopolyploid | |
| CN104255443B (en) | A kind of polymerization is containing the seed selection method for rice of anti-three kinds of disease genes | |
| CN107435079A (en) | Utilize the method for the wide affine rice material of Anther Culture quick breeding | |
| CN110100723A (en) | A kind of hybrid selection method and its application of fast period cabbage type rape | |
| CN105142394A (en) | Brassica plants resistant to clubroot | |
| CN104521744B (en) | A kind of bacterial leaf spot resistance Japonica two line hybrid rice method | |
| CN102144560B (en) | Method and application method for obtaining novel germ plasm of brassica A genome vegetable | |
| CN109042304B (en) | Breeding method of rice blast resistant high-quality high-yield two-line hybrid indica rice combination | |
| CN106489716A (en) | A kind of Rice Resistance linum leaf spot breeding method | |
| KR102276931B1 (en) | New indica rice variety 'Sejong Indi 1' having excellent submergence tolerance, anaerobic germinability and blast resistance and breeding method thereof | |
| CN104521758B (en) | A kind of Xian Jing hand over gene introgression blast resisting breeding new method | |
| CN101810136A (en) | Cauliflower cytoplasmic male sterile line breeding method and application of male sterile line | |
| CN109329046B (en) | Breeding method of Chancalyx and TYLCV-resistant cherry tomato inbred line | |
| CN108770678B (en) | Cross breeding and breeding method of sea rice and japonica rice restorer | |
| CN103141376A (en) | Cultivation method of rice germplasm with high-resistant starch and low-amylose starch | |
| CN101886083B (en) | Application of rice OsEATB gene in modifying rice yield traits | |
| CN111357642B (en) | Breeding method of Chinese cabbages | |
| CN103798125A (en) | Method for acquiring novel species of brassicaceous vegetables and application method of novel specie of brassicaceous vegetables | |
| CN108243949B (en) | Breeding method of light and simple type temperature-sensitive genic male sterile line | |
| CN106489727A (en) | A kind of bacterial Footrot resistance Japonica two line hybrid rice method | |
| CN105104185B (en) | Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving | |
| CN106342680A (en) | Method suitable for cultivation of various genotype wheat microspores |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |