CN1159879A - Technique for breeding of hybridized rice - Google Patents
Technique for breeding of hybridized rice Download PDFInfo
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- CN1159879A CN1159879A CN 97100192 CN97100192A CN1159879A CN 1159879 A CN1159879 A CN 1159879A CN 97100192 CN97100192 CN 97100192 CN 97100192 A CN97100192 A CN 97100192A CN 1159879 A CN1159879 A CN 1159879A
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Abstract
A breeding technique for hybrid paddy rice uses broad-congenial round-grained non-glutinous rice "02428" as female parent and strong recovery long-grained non-glutinous rice "Gui 630" as male parent and includes hybridization between subspecies of said rice varieties, separation and selection breeding, tissue culture, stabilizing to obtain recovery line"Luhui 17" which has high combining ability and recovery power, and strong adaptability, and hybridizing with proper sterile line (including amphibolic karyosterile line) to obtain many varieties of hybrid paddy rice featuring early mature, high yield and high quality.
Description
The invention belongs to the rice heterosis utilization field, be specifically related to the breeding method of hybrid rice.
China's hybrid rice area under cultivation has about 0.16 hundred million hectare, account for national paddy rice cultivated area 50%, compare with the conventional rice kind, hybrid rice generally can be increased production about 10%, because like this, development of hybrid rice and popularization have been acknowledged as China paddy rice in modern age and have bred a important milestone on the history.But paddy rice is the same with other crop, and heterotic power is limited by the genetic variation and genetic differentiation level of parental gene, and China hybrid rice of promoting at present is because the reproductive disorder between indica and japonica subspecies all can only utilize the hybrid vigour in the same subspecies.Therefore since the middle and later periods eighties, a collection of strong advantage combination was promoted, although number of research projects has been done by breeding men.But yield level does not have new breakthrough, and along with agricultural develops to highly efficient and productive, high-quality direction, particularly China is faced with the serious situation that the people subtracts with increasing.Guarantee and improve the demand that total grain output adapts to development, seed selection has the super high-yielding yield level, and adaptability is strong, and the new combination of the hybrid rice of disease-resistant high-quality has become the great brainstorm subject on the rice breeding.Powerful hybrid vigour between indica and japonica subspecies is early paid attention to for scientists, but the breeding time that Hybrids of Indica and Japonica exists is long, and solid rate variance enriches the not full physiology reproductive disorder that waits and influenced heterotic effective utilization.The rice breeding worker has made number of research projects in this respect, has obtained significant achievement, and external breeding man successfully selects the super high-yielding kind that the Xian round-grained rice is handed over, as international paddy rice " super hybridization rice ".But these kinds can not be at Chinese popularizing planting owing to adaptability problem, and therefore, the super high-yielding kind (combination) of cultivating China is the task of top priority.
The objective of the invention is to solve the problems referred to above that the Xian round-grained rice mingles paddy rice, a kind of high-combining ability is provided, super high-yielding, disease-resistant, high-quality, adaptability are strong, and part is utilized the breeding new material of the hybrid rice of indica-japonica heterosis, cultivates the super high-yielding combination and goes up large-area applications for producing.
The object of the present invention is achieved like this.
The breeding new material of the new combination of this hybrid rice comprises and selects to have the japonica rice material of wide affinity characteristic and the indica type material with strong restoring force.With partly the transformation of japonica rice gene is to good indica restorer line, the middle generation is selected to have round-grained rice type blood relationship indica material and carries out tissue culture and the selfing of Duo Dai purifying with its wide compatibility gene, and stable back is used as the parent and prepares combination.
A kind of technique for breeding of hybridized rice is characterized in that being realized by following steps;
(1) be female parent with wide affine japonica rice variety " 02428 ", recovering system's " Gui 630 " with strong restoring force long-grained nonglutinous rice is that male parent is hybridized, and selects that coordinate force is good the middle generation, and the combo advantage is strong has round-grained rice type blood relationship indica material 717.
(2) material 717 serve as for this to have in the round-grained rice type blood relationship indica from generation to generation, carry out the anther tissue culture purified after, to breed the high-combining ability recovery be Lu extensive 17 selfing many generations again.
(3) being female parent with round-grained rice matter Xian caryon sterile line K17A, is that Lu extensive 17 is male parent preparing hybrid combination " K excellent 17 " to recover.
Parental source and feature thereof:
Seed selection has the recovery system that the Xian round-grained rice is handed over the hybrid rice combination of strong advantage, and it is crucial that selected parent and offspring select stable.By evaluation and screening to 278 parts of japonica rice resources, japonica rice variety 02428 has wide compatibility gene, extremely strong with indica hybrid hybrid vigour, the biological yield height, but because of not having present three is that the strong recovery gene of male sterile line can not utilize on ternary hybrid rice, promptly select for use indica restorer line Gui 630 to hybridize with it for male parent, Gui 630 has strong recovery gene, characteristics such as work good.Selecting indica strain system to cultivate through anther tissue in filial generation quickens to stablize, it is good to breed coordinate force, and adaptability is strong, and it is Lu extensive 17 that pollen amount Xian round-grained rice too hands over indica to recover, identify that through coordinate force and adaptability Lu extensive 17 is recovery systems of desirable ternary hybrid rice.
The feature of Lu extensive 17: about plant height 115cm, plant is neat, and tillering ability is strong, the stem number of blade 15～16 leaves, duration from seeding to heading 105 days.Ye Selve is dark green, the leaf bud sheath, and leaf margin, auricle, internode, stem are all colourless, and fringe is spindle, spike length 25cm, every fringe 150 of grains, ripening rate 90%, thousand kernel weight 30 grams, grain is light yellow, grain length 0.91cm, the short awns in minority top, the grain husk point is colourless, the heading neat and consistent, flower pesticide is big and full, and pollen amount is big, and main rice matter index reaches " edible high quality rice " promulgated by the ministries or commissions of the Central Government secondary superior rice standard, the blast resisting ability is strong, and is resistant to lodging, and the later stage annesl is good.
The feature of the combination K excellent 17 of extensive 17 preparations in Lu:
About plant height 110cm, straight when bar is endured, the plant type compactness, tillering ability is strong, and effectively fringe is many, and the big grain of fringe is heavy, kipeming high yield, thousand kernel weight 30 grams, rice matter is better, and the blast resisting ability is stronger, and the cold-resistant fringe phase in seedling stage is high temperature resistant, the ripe phase discolors, and is resistant to lodging, wide adaptability, hybrid seed yield height.
Advantage of the present invention and good effect.
(1) yield level height: the recovery that the present invention breeds is to work good in Shanghai extensive 17, the hybrid combination performance of being prepared is by the yield level of high yield, K excellent 17 is in the trivial examination of nineteen ninety-five south rice, gather average per hectare and produce 9010 kilograms, comparison is according to excellent 64 volume increase 23.09% of kind of prestige, the daily output is up to 0.29 kilogram/hectare, and these three indexs are created all-time high in combination of the same type.
(2) wide adaptability: K excellent 17 all than plant combination performance volume increase as the landlord, in southern rice district, obtains high yield from 300 meters to 800 meters equal can plantations of height above sea level in trivial examination of southern rice and demonstration plant.
(3) resistance and better resistance: K excellent 17 seedling stages are low temperature resistant, the fringe phase is high temperature resistant, and lodging tolerance is strong.Blast resisting, 4 grades of seedling leaf pest, 5 grades of neck pest.The neck pest incidence of disease 12.15%, and contrast 9 grades of short excellent S leaf pest, the neck pest incidence of disease 98%.
(4) hybrid seed yield height: K excellent 17 is because of maternal flowering habit is good, and the paternal pollen amount is big.Easily obtain high yield in the production of hybrid seeds, hybrid seed yield generally can reach 4500 kilograms/hectare, and comparison improves 40～50% according to Shanyou 63.
Accompanying drawing is a concrete breeding line spectrogram of the present invention.
This hybrid rice new combination material is take wide affine japonica rice variety " 02428 " as maternal, and strong restoring force Indica Rice Restorer Lines " Gui 630 " is hybridized for male parent. Selfing is selected to such an extent that have a round-grained rice type blood relationship indica material 717 after 7 generations again, organizes cultivation with flower training technology again, acceleration for stabilization, and then in 3 generations of selfing, bred high-combining ability restorer Lu extensive 17. Take round-grained rice matter Xian line with genic sterile K17A as maternal, extensive 17 are male parent preparing hybrid combination " K excellent 17 " take the restorer Lu.
Specific implementation method of the present invention is described in detail in detail below.
The different friendship habit of the maternal K17A of K excellent 17 is good, and the bag neck is light, and the pollination attitude is good; stigma exposing ratio is up to 95%, and two exposing can reach about 75%, and vitality of stigma is strong; Zao about 1 hour when flower than precious oily 97A; ripening rate is about 80% when normal flower synchronization, and extensive 17 tillering abilities in male parent Lu are strong, and the many fringes of fringe are big; flower pesticide is full; pollen amount is big, and the production of hybrid seeds is easy, and this combination production of hybrid seeds generally can reach more than 300 kilograms.The seeding technique main points are:
1, broadcast the poor phase: in the Luzhou, Sichuan and identical ecotope adopt the male parent production of hybrid seeds of two phases, the male parent first phase is in the last ten-days period in March sowings, the second phase is every 6 days, maternal date of seeding, be as the criterion during with male parent age, sows when promptly male parent first phase leaf age reaches 6,7 leaves, the time difference is 40～42 days.
2, the male parent hectare amount of broadcasting is 0.027～0.033 kilogram, broadcasts preferablely with 13.33 * 13.33cm two point, helps the strong seedling of the many tillers of male parent.0.233 kilogram of the maternal hectare amount of broadcasting, suitably light seeding.
3, seed farm is planted father and mother's one's own profession than 2: 12 or 2: 14.
4, responsive to " 920 " because of female parent, using of " 920 " is lighter than other male sterile line, and general hectare restrains " 920 " with 0.8.
5, " excellent 63 production of hybrid seeds are identical with Shan for other control measures.
Table 1 " K excellent 17 " and the output comparison sheet that contrasts
| Identify unit | The name of an article | Paddy per mu yield (Kg) | The paddy daily output (kg) | Brown rice per mu yield (Kg) |
| In the examination of indica hybrid rice district, the whole nation Xian early in ripe group | Excellent 64 (the CK) ± % of excellent 17 prestige of K | 600.67 488.00 +23.09 | 4.48 3.91 +14.58 | 486.36 388.79 +25.10 |
| In provinces and regions, the Sichuan examinations among the Xian ripe group | Excellent 195 (the CK) ± % of excellent 17 Shans of K | 563.31 478.92 +17.62 | 4.02 3.54 +13.56 | 450.65 387.92 +16.17 |
Claims (3)
- A kind of technique for breeding of hybridized rice is characterized in that being realized by following steps:(1) be female parent with wide affine japonica rice variety " 02428 ", recovering system's " Gui 630 " with strong restoring force long-grained nonglutinous rice is that male parent is hybridized, and selects that coordinate force is good the middle generation, and the combo advantage is strong has round-grained rice type blood relationship indica material 717;
- (2) material 717 serve as for this to have in the round-grained rice type blood relationship indica from generation to generation, carry out the anther tissue culture purified after, to breed the high-combining ability recovery be Lu extensive 17 selfing many generations again;
- (3) being female parent with round-grained rice matter Xian caryon sterile line K17A, is that Lu extensive 17 is male parent preparing hybrid combination " K excellent 17 " to recover.
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| Application Number | Priority Date | Filing Date | Title |
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| CN97100192A CN1075346C (en) | 1997-01-17 | 1997-01-17 | Technique for breeding of hybridized rice |
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| CN97100192A CN1075346C (en) | 1997-01-17 | 1997-01-17 | Technique for breeding of hybridized rice |
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| CN1159879A true CN1159879A (en) | 1997-09-24 |
| CN1075346C CN1075346C (en) | 2001-11-28 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
| CN103461111A (en) * | 2013-09-27 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for high-cold-resistance rice |
| US8743238B2 (en) | 2010-11-30 | 2014-06-03 | Fujifilm Corporation | Image processing apparatus, imaging apparatus, image processing method, and white balance adjustment method |
| CN104429932A (en) * | 2015-01-04 | 2015-03-25 | 王开 | Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method |
| CN104521744A (en) * | 2015-01-04 | 2015-04-22 | 黎建波 | New bacterial-blight-resisting japonica rice breeding method |
| CN104521758A (en) * | 2015-01-04 | 2015-04-22 | 安徽袁粮水稻产业有限公司 | New breeding method for resisting rice blast through indica-japonica hybrid gene introgression |
| CN104542256A (en) * | 2015-01-04 | 2015-04-29 | 陈丁龙 | Breeding method for importing false smut resistance of indica rice into japonica rice |
| CN106489727A (en) * | 2015-09-08 | 2017-03-15 | 无锡南理工科技发展有限公司 | A kind of bacterial Footrot resistance Japonica two line hybrid rice method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210844B (en) * | 2013-04-23 | 2014-05-21 | 江苏徐淮地区徐州农业科学研究所 | Method for improving frequency of callus induction and plant differentiation rate in japonica rice anther culture |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1014298B (en) * | 1990-03-09 | 1991-10-16 | 江苏省农业科学院粮食作物研究所 | Method of producing hybrid rice between subspecies of long-grained and round-grained nonglutinous rice |
| CN1028471C (en) * | 1990-07-17 | 1995-05-24 | 邓祚荣 | Two system method crossbreeding technology for long-grained nonglutinous rice subspecised |
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1997
- 1997-01-17 CN CN97100192A patent/CN1075346C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8743238B2 (en) | 2010-11-30 | 2014-06-03 | Fujifilm Corporation | Image processing apparatus, imaging apparatus, image processing method, and white balance adjustment method |
| CN102577933A (en) * | 2012-03-20 | 2012-07-18 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
| CN102577933B (en) * | 2012-03-20 | 2014-05-21 | 安徽绿亿种业有限公司 | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method |
| CN103461111A (en) * | 2013-09-27 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for high-cold-resistance rice |
| CN103461111B (en) * | 2013-09-27 | 2015-07-22 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for high-cold-resistance rice |
| CN104521758A (en) * | 2015-01-04 | 2015-04-22 | 安徽袁粮水稻产业有限公司 | New breeding method for resisting rice blast through indica-japonica hybrid gene introgression |
| CN104521744A (en) * | 2015-01-04 | 2015-04-22 | 黎建波 | New bacterial-blight-resisting japonica rice breeding method |
| CN104542256A (en) * | 2015-01-04 | 2015-04-29 | 陈丁龙 | Breeding method for importing false smut resistance of indica rice into japonica rice |
| CN104429932A (en) * | 2015-01-04 | 2015-03-25 | 王开 | Novel indica-japonica hybrid gene introgression sheath blight resistance breeding method |
| CN104521758B (en) * | 2015-01-04 | 2017-03-29 | 安徽袁粮水稻产业有限公司 | A kind of Xian Jing hand over gene introgression blast resisting breeding new method |
| CN104521744B (en) * | 2015-01-04 | 2017-06-13 | 浙江原态农业股份有限公司 | A kind of bacterial leaf spot resistance Japonica two line hybrid rice method |
| CN104429932B (en) * | 2015-01-04 | 2017-06-20 | 兰进好 | A kind of Xian round-grained rice hands over the anti-banded sclerotial blight breeding new method of gene introgression |
| CN106489727A (en) * | 2015-09-08 | 2017-03-15 | 无锡南理工科技发展有限公司 | A kind of bacterial Footrot resistance Japonica two line hybrid rice method |
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| CN1075346C (en) | 2001-11-28 |
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