CN1669407A - Purification and pure state preservation method of rose violet type sterile line parental generation - Google Patents
Purification and pure state preservation method of rose violet type sterile line parental generation Download PDFInfo
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- CN1669407A CN1669407A CN 200510038778 CN200510038778A CN1669407A CN 1669407 A CN1669407 A CN 1669407A CN 200510038778 CN200510038778 CN 200510038778 CN 200510038778 A CN200510038778 A CN 200510038778A CN 1669407 A CN1669407 A CN 1669407A
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Abstract
The invention discloses a purification and purification protection method of rose violet infertility parental generation, its seed production pureness completely reaches the state-specified commercial application standard, it belongs to the ternary hybrid breeding field. The invention comprises: cultivate sterility maternal A and retention paternal B in the absolute isolated condition, the maternal and the paternal are duplicating planted together, cover the flower duplicated strains in the bag to finish the duplicated cross of maternal and paternal single strain; the gained seeds are cultivated in the absolute isolated condition to form duplicated cross strain series, check the sterility strain series in the field, pick the seeds whose pureness is above 99.9 percent and contains the original farming description to breed the seed of next generation.
Description
Technical field
Pure method is specifically protected for red lotus type male sterile line parent's purification in numerous kind of field of the Three-line Hybrid system of the invention belongs to.
Background technology
The hybrid rice technology of China can be divided into two line method and three line method two classes.The present invention relates to the Three-line Hybrid seeding technique.
China is since 1973 successfully since the invention ternary hybrid rice, use wild abortion type sterile series in the production always, this hereditary unicity tendency has hidden danger to Rice Production, therefore seeks and excavate the male sterile line in different cytoplasm source, is China rice breeding scientific research personnel's effort target always.Red lotus type male sterile line (1972) (latest report with the similar time discovery of wild abortion type sterile series, Xinhua News Agency's report on September 23rd, 2003, topic: China's red lotus type hybrid rice research obtains important breakthrough), have the cytoplasmic sterility gene different, but because the problem of purity never has by production application with wild abortion type sterile series.
Summary of the invention
The objective of the invention is at red lotus type male sterile line in the prior art because of the low problem that can not be applied in the actual production of purity, provide a kind of and can make the red lotus type male sterile line parent's of the commercial application standard that purity reaches national regulation fully purification protect pure method.
Purpose of the present invention can reach by following measure:
Pure method is protected in red lotus type male sterile line parent's purification, and this method comprises:
(1) maternal A of cultivation male sterile line and maintenance line male parent B under absolute isolation condition, maternal planting in pairs with male parent one cave one seedling is in the same place, and paired ear part bagging of florescence is handed in pairs to finish this individual plant of father and mother;
(2) the individual plant of receiving seed cultivation under absolute isolation condition of handing in pairs of future generation, be formed into handing over strain system, in the field sterile strain system is tested, receive purity 99.9% or more, the economical character seed that keeps the qualified strain of former male sterile line to be, breed breeder's stock of future generation.
Pure method is protected in red lotus type male sterile line parent's of the present invention purification, and wherein male sterile line is selected the safe A in red lotus type male sterile line Guangdong for use, and maintenance line is selected the safe B in Guangdong for use.
This method also comprises with the breeder's stock that makes breeds original seed of future generation.Wherein the breeding of breeder's stock and original seed is to make female parent with the red lotus type male sterile line, and maintenance line is bred as male parent, comprises safe isolation, proper seeding, row ratio, gibberellin use and artificial pollination, disease control, roguing measure rationally.Wherein safety is isolated into: japonica rice district＞1000 meter, long-grained nonglutinous rice district＞500 meter, or to be separated by other rice varieties of heading flowering period carries out production of hybrid seeds isolation more than 20 days.Wherein the gibberellin consumption is every mu of 10-12 gram.
This method also comprise the qualified strain of getting respectively in the paired friendship strain system be the father and mother that hand in pairs as individual plant of the seed mixture of maternal A and male parent B this, the circulation that forms red lotus type male sterile line parent is purified.This method comprises that also finishing individual plant every year at least hands over 50 pairs in pairs.
The purification of the present invention and wild scum of a community's type male sterile line with breed the following characteristics of having compared:
(1) aspect the male sterile line purification, at red lotus type stigma exposing ratio of sterile line height, the characteristics that outcrossing seed-setting rate is high, set up the circulation purification of " friendship one is two numerous usefulness " and protected pure system, once hand over strain system breeding to substitute with hybrid seeding to the 5th in pairs, the sterile strain system after the 5th generation can not be in order to hybrid seeding.Once hand in pairs breeding to the 4th on behalf of a samsara, year multiple samsara, definitely having every year the 5th generation to hand over sterile strain in pairs is that seed is in order to hybrid seeding.Wherein said " one hands over " refers to paired friendship; " one is " refers to hand in pairs strain system; " two is numerous " refers to the breeding of breeder's stock, original seed; " usefulness " refers to be used for hybrid seeding.
And wild abortion type sterile series is handed in pairs and is carried pure strain and can breed substitute with hybrid seeding more.
(2) numerous kind of cost is low, shows as the reproductive output height, about 250 kilograms of average yield per mus, and gibberellin (920) consumption is few.
The present invention is owing to adopt the circulation purification technique, and parental purity reaches more than 99.8%, thereby guarantees that seed production purity is stabilized in more than 98%, reaches the commercial application standard (country-level kind of standard) of national regulation fully; Because male sterile line phimosis degree is little, " 920 " consumption has only general three to be about 1/3 of male sterile line, cost is low.
Description of drawings
Fig. 1 protects the schematic diagram of pure method for red lotus type male sterile line parent's purification.
Embodiment
Pure method is protected in red lotus type male sterile line parent's of the present invention purification, as shown in Figure 1, be specially: cultivating male sterile line every year under absolute isolation condition, the safe B in Guangdong that serves as maternal and maintenance line with the safe A in the Guangdong of red lotus type male sterile line is a male parent, maternal planting in pairs with male parent one cave one seedling is in the same place, female parent generally shifts to an earlier date 3-5 days than male parent sowing time, and to guarantee father and mother's flower synchronization originally, paired ear part bagging of florescence is handed in pairs to finish this individual plant of father and mother; The individual plant of receiving hands over seed cultivation under absolute isolation condition of future generation in pairs, be formed into handing over strain system, in the field sterile strain system is tested, receive purity 99.9% or more, the economical character seed that keeps the qualified strain of former male sterile line to be, be used to breed breeder's stock of future generation, utilize the breeder's stock that makes to breed original seed of future generation again, the original seed quantity that makes is just relatively large, and the safe A original seed in the Guangdong that makes just can be used for large-area system hybrid rice seed.
Safe A is a female parent with the Guangdong of red lotus type male sterile line, the safe B in the Guangdong of maintenance line is a male parent, make the propagation technique of breeder's stock and original seed, because the red lotus type male sterile line is compared the characteristics that stigma exposing ratio extra-high-speed (100%) and outcrossing seed-setting rate height (about 70%) are arranged with wild abortion type sterile series, so the red lotus type hybrid seeding is compared with wild abortion type mainly to use in isolated area setting and " 920 " difference is arranged.In addition, on the pollen abortion time, red lotus type male sterile line (two nuclear phases) and wild abortion type sterile series (monokaryotic stage) have the difference of matter, so the impurity elimination technology that red lotus type male sterile line field similar shape can be educated strain is different from wild abortion type sterile series.
Except that These characteristics, other such as proper seeding, optimum period transplant, row ratio, florescence control, artificial supplementary pollination rationally, to prevent and treat supplementary measures such as banded sclerotial blight and kernel smut similar to the wild abortion type hybrid seeding.
Continuous 3 years facts have proved, uses this invention, and the red lotus type hybridization seed production purity reliably reaches more than 98%, and hybrid seed yield is stable to reach 250 kilograms/mu.
The propagation method of safe B breeder's stock in safe A in red lotus type male sterile line Guangdong and maintenance line Guangdong and original seed: safe isolation distance japonica rice district＞1000 meters, long-grained nonglutinous rice district＞500 meter.Sowing in maternal 6 days in advance, male parent divided for two phases, and the I phase, the II phase was apart from sowing in maternal 6 days apart from sowing in maternal 3 days.This transplanting method of father and mother and row be than 1: 6,12 * 16.5 centimetres of this seeding row spacings of father and mother, and this leaves the operation road that delegation widens every father and mother.A seedling is transplanted in every cave.Use twice of jinggangmeisu class chemical control banded sclerotial blight in meiosis stage (sword-like leave of full field 30-50% stem evil is all extracted out) and cut phase respectively.When the 10% stem evil cut of full field, cut leaf and handle, use every mu of 10-12 gram of gibberellin (920) to spray immediately.Respectively when the 30-50% stem evil cut of full field and during neat fringe with the black clever class chemical control kernel smut twice of going out, add wherein that to beat the powder rust clever during the full heading time medication.In the whole growing, pull out the discrepant plant of the safe A of morphological characters and Guangdong in the maternal row, contain and spend back 28 days timely results hybrid seeds.
Utilize the technical characterictic of the breading protospecies hybrid rice seed that method of the present invention makes:
(1) safety is isolated: japonica rice district＞1000 meter, long-grained nonglutinous rice district＞500 meter, or to be separated by other rice varieties of heading flowering period carries out numerous kind of isolation more than 20 days.
(2) begin sowing in good time: male parent is divided the sowing of two phases, and I phase male parent fell behind 3 days than maternal, II phase male parent distance I phase male parent 3 days.
(3) plant density, father and mother row ratio: this transplanting method of father and mother and row be than 1: 6,12 * 16.5 centimetres of this seeding row spacings of father and mother, and this leaves the operation road that delegation widens every father and mother.A seedling is transplanted in every cave.
(4) gibberellin 920 uses and artificial supplementary pollination: cut leaf and handle when the 10% stem tiller cut of full field, use every mu of 10-12 gram of gibberellin (920) to spray immediately.Customary impurity elimination after catch up with pollen to carry out artificial supplementary pollination when spending every day.
(5) disease control: use twice of jinggangmeisu class chemical control banded sclerotial blight in meiosis stage (sword-like leave of full field 30-50% stem tiller is all extracted out) and cut phase respectively.Respectively when the 30-50% stem evil cut of full field and during neat fringe with the black clever class chemical control kernel smut twice of going out, add wherein that to beat the powder rust clever during the full heading time medication.
(6) in the whole growing, pull out the discrepant plant of the safe A of morphological characters and Guangdong in the maternal row.The homotype impurity elimination that can educate strain of when flower every day sees Table 1.Sheng is spent back 28 days and is in time gathered in the crops hybrid seed.
Normal strain of the safe A male sterile line in table 1 Guangdong and the contrast identification that can educate assorted strain
| Can educate assorted strain | Sterile normal strain |
| 1. the heading of 1 day ahead of time florescence is done sth. in advance 0.5～1 hour during flower.2. flower pesticide stretches out behind the clever shell to touch with hand and promptly sees the vaporific pollen of scattering.3. ghost flower pesticide slightly was white in color the same day after the loose powder.4. filigree continues elongation back ghost flower pesticide has levitation 5. to spend the 2nd day withered brown of residual flower pesticide in back, the adhesion sense is arranged, be irregular shape. | 1. the florescence falls behind heading in 1 day, falls behind 0.5～1 hour during flower.2. flower pesticide stretches out behind the clever shell to touch with hand and loses loose powder, or only sees a small amount of non-vaporific pollen that is scattered (sterile).3. the not thorough flower pesticide of solid flower pesticide or loose powder slightly was yellow the same day.4. filigree continues still to see in the flower pesticide of elongation back has remnants (sterile) pollen grain, flower pesticide to have sagging (sense) 5. to spend the 2nd day residual flower pesticide withered yellow in back, no adhesion sense still is small rod. |
Utilize red lotus type male sterile line Guangdong safe A work female parent, the safe B in maintenance line Guangdong to make male parent circulation test cross, and test cross kind (original seed and breeder's stock) is bred among the present invention.It is maternal wherein to utilize the safe A in red lotus type male sterile line Guangdong to do, and can be this preparing hybrid of father and mother seed with long-grained nonglutinous rice R938.
The successful Application of the safe A in the parent Guangdong that the present invention makes on hybrid seeding is as the application on system crossbreed Guangdong excellent 938.The male sterile line of the present invention and wild scum of a community's type has following characteristics comparing aspect the hybrid seeding:
1, hybrid seed yield Gao Erwen: the present invention uses the safe A in red lotus type male sterile line Guangdong different preferably friendship flowering habit, and clever shell opening angle is big, and stigma exposing ratio almost 100%, two exposing ratio reaches more than 65% the outcrossing seed-setting rate height.The male sterile line big panicle many grains per panicle adds good different friendship, 260 kilograms of production of hybrid seeds per mu yields, and 370 kilograms of high-yield field pieces are the high 20-30% of group than similar three.
2, breeding cost is low: the safe A amount of application of nitrogen fertilizer in the red lotus type male sterile line Guangdong that the present invention uses is that male sterile line lacks 15-20% than general three, " 920 " consumption only needs three to be about 30% of male sterile line, therefore when the present invention used the safe A in red lotus type male sterile line Guangdong and carries out the hybrid rice production of hybrid seeds, production cost was that male sterile line descends 50% than general three.
Claims (8)
1, pure method is protected in red lotus type male sterile line parent's purification, and this method comprises:
(1) maternal A of cultivation male sterile line and maintenance line male parent B under absolute isolation condition, maternal planting in pairs with male parent one cave one seedling is in the same place, and paired ear part bagging of florescence is handed in pairs to finish this individual plant of father and mother;
(2) the individual plant of receiving seed cultivation under absolute isolation condition of handing in pairs of future generation, be formed into handing over strain system, in the field sterile strain system is tested, receive purity 99.9% or more, the economical character seed that keeps the qualified strain of former male sterile line to be, breed breeder's stock of future generation.
2, pure method is protected in purification according to claim 1, and wherein the maternal A of male sterile line selects the safe A in Guangdong of red lotus type male sterile line for use, and maintenance line male parent B selects the safe B in the Guangdong of maintenance line for use.
3, pure method is protected in purification according to claim 1 and 2, and this method also comprises with the breeder's stock that makes breeds original seed of future generation.
4, pure method is protected in purification according to claim 3, wherein the breeding of breeder's stock and original seed is to make female parent with the red lotus type male sterile line, maintenance line is bred as male parent, comprises safe isolation, proper seeding, row ratio, gibberellin use and artificial pollination, disease control, roguing measure rationally.
5, pure method is protected in purification according to claim 4, and wherein safety is isolated into: japonica rice district＞1000 meter, long-grained nonglutinous rice district＞500 meter, or to be separated by other rice varieties of heading flowering period carries out production of hybrid seeds isolation more than 20 days.
6, pure method is protected in purification according to claim 4, and wherein the gibberellin consumption is every mu of 10-12 gram.
7, pure method is protected in purification according to claim 1, this method also comprise the qualified strain of getting respectively in the paired friendship strain system be the father and mother that hand in pairs as individual plant next year of the seed mixture of maternal A and male parent B this, the circulation that forms red lotus type male sterile line parent is purified.
8, pure method is protected in purification according to claim 1, and this method comprises that also finishing individual plant every year at least hands over 50 pairs in pairs.
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| CN 200510038778 CN1669407A (en) | 2005-04-08 | 2005-04-08 | Purification and pure state preservation method of rose violet type sterile line parental generation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006463A (en) * | 2018-08-13 | 2018-12-18 | 辽宁省水稻研究所 | A kind of propagation method of sterile line of hybridized rice original silkworm egg |
| CN113367062A (en) * | 2021-06-29 | 2021-09-10 | 高邮周邶墩农业发展有限公司 | Method for improving purity of japonica rice sterile line |
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2005
- 2005-04-08 CN CN 200510038778 patent/CN1669407A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006463A (en) * | 2018-08-13 | 2018-12-18 | 辽宁省水稻研究所 | A kind of propagation method of sterile line of hybridized rice original silkworm egg |
| CN113367062A (en) * | 2021-06-29 | 2021-09-10 | 高邮周邶墩农业发展有限公司 | Method for improving purity of japonica rice sterile line |
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