CN101697706B - Application and cultivation method of high-frequency recessive female sterile rice restorer lines - Google Patents
Application and cultivation method of high-frequency recessive female sterile rice restorer lines Download PDFInfo
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Abstract
The invention discloses application of high-frequency recessive female sterile rice restorer lines in mixed-sowing or mixed-harvesting type rice seed production. A specific method of the application comprises the steps of taking a high-frequency recessive female sterile rice restorer line as a male parent, taking a hybrid rice male sterile line as a female parent, performing mixed sowing on male/female-parent seeds and performing mixed harvesting on the male/female-parent seeds subjected to hybridization and completing the process of seed production, or the steps of taking the hybrid rice male sterile line with screenable genetic specific characters as the female parent, performing mixed sowing and mixed harvesting and then adopting different separation methods to perform further impurity removal on the seeds subjected to mixed harvesting. The invention also provides a cultivation method which hybridizes a female sterile restorer line FSV1 with a conventional hybrid rice restorer line R, chooses and gets the high-frequency recessive female sterile rice restorer line with stable genetic characters and excellent comprehensive agronomic characters from F1 progenies. The method has the advantages of realizing the full-mechanized operation of producing and processing hybrid rice seeds and greatly improving the efficiency of hybrid rice seed production.
Description
Technical field
The present invention relates to application and the breeding method thereof of a kind of hybrid rice, relate in particular to a kind of application and breeding method of hybrid rice restoring line.
Background technology
Hybrid rice is a flag of China's agricultural science and technology.Hybrid rice is promoted from beginning large tracts of land in 1976 and adds up between so far more than 30 year to plant more than 60 hundred million mu, and accumulative total is increased production more than 6,000 hundred million kilograms of paddy; In recent years cultivated area accounts for about 60% of the national paddy rice gross area, and output accounts for about 67% of paddy rice gross output.We can say that the hybrid rice industry is the national industry and the international famous brand of China, planting industry science and technology is the core of hybrid rice science and technology.Promote that further developing of hybrid rice kind industry science and technology is to keep China hybrid rice first place in the world and the strategic measure of guaranteeing China's grain security.
The biparent cross first generation (F1) hybrid vigour that hybrid rice utilizes just can produce proterties to the second generation (F2) and separate, and therefore, the production of hybrid seeds every year could ensure that the land for growing field crops produces with planting.Existing breeding technology for hybrid rice mainly is with male sterile line (female parent) and male sterile restoring line (male parent); Than alternate plantation, hybrid seed on the male sterile line and male parent selfed seed be harvesting (male parent also can just slice off after having caught up with powder) separately respectively according to certain row.Traditional lengthy and tedious complicacy of production of hybrid seeds process, labour intensity is big, inefficiency.
Along with the acceleration of social development, agricultural modernization process, China rural laborer worsening shortages, the in fact continuous atrophy of the area of base of China's production of hybrid seeds in recent years; Foreign in addition multinational garrison constitutes grave danger to the survival and development of China's hybrid rice kind industry; The severe situation of this " interior tired outer sorrow "; Requirement is reformed China's production technology of hybridized rice seed, realizes seed production and the omnidistance modernization of processing.
For this reason, many scientific workers have carried out correlative study and technological innovation:
At application number is in the application for a patent for invention of CN97107633.2 " a kind of breeding technology for hybrid rice of suitable for mechanization operation "; Wanxian City Inst. of Agricultural Science, Sichuan Prov. has proposed the father and mother this part seeding technique same period, explains that it is feasible carrying out breeding of hybrid rice through the sowing same period; It mainly is to utilize grain look between hybrid seed and male parent, grain weight, particle shape to differ greatly, and separates hybrid seed and male parent through optical instrument or mechanical means again.It is the system of mixing that this patented method is used normal conventional recovery of female fertility, mixes and receives male parent seed content height in the seed; Separating method is complicated operation not only, and equipment has high input, and relies on a grain look, grain reliability heavy, that particle shape separates not high, obtains the high-purity hybrid seed and has certain degree of difficulty.
At application number is in the application for a patent for invention of CN99115125.9 " chemical roguing technology for breeding hybrid rice "; Inst. of Paddy Rice, Sichuan Agriculture Univ. proposes anti-herbicide gene is imported the maintenance line of ternary hybrid rice; Make maintenance line obtain anti-herbicide gene, possess the characteristic of antiweed; Become the production of hybrid seeds to use male sterile line with this maintenance line with the male sterile line outbreeding that does not contain anti-herbicide gene of homotype, make the male sterile line of breeding have the antiweed characteristic; Is that trilinear hybrid rice seed is produced in hybridization with the male sterile line with antiweed characteristic with recovering.Adopt this kind technology to reach the purpose of production of hybrid seeds time and labour saving, raising seed purity through going out except that the assorted seedling in the male sterile line seedling at male sterile line seedling spraying weed killer herbicide.Though the assorted technical measures of male sterile line when this technology has proposed to utilize weed killer herbicide to remove the production of hybrid seeds, this technology mainly are the production for hybrid rice seed highly purified male sterile line rice shoot is provided, and this only is one of condition that guarantees hybrid seed purity.In addition, for how reforming seeding technique, how improving problems such as production of hybrid seeds process efficiency, all do not relate in this patent application.
At application number is in the application for a patent for invention of CN00114492.8 " decontaminating and purity maintaining technology for three-line hybrid rice "; A kind of method of removing the seedling of the maternal male sterile line of sneaking in the Three-line Hybrid paddy rice seedling and maintenance line thereof with the weed killer herbicide Bentazon has been proposed: select to have maintenance line and the cytoplasmic nuclear male sterile line outbreeding production male-sterile seed that has equally the recessive character of isozygotying of Bentazon sensitive lethality to the recessive character of isozygotying of Bentazon sensitive lethality; Using male sterile line of being produced and the recovery that does not have said stealthy proterties is the preparing hybrid rice, after planting sprays 0.001～1.0% Bentazon solution at hybrid rice seed.This patent application points out that its method can be applied in the production of hybrid seeds of Three-line Hybrid paddy rice or hybrid seed purity Rapid identification.This patent has proposed to utilize weed killer herbicide to go out except that the male sterile line selfing seedling to the Bentazon sensitive lethality contained in the hybrid, does not but relate to (can not realize) this mixed seeding of father and mother and mixes the receipts seeding technique.
At application number is in the application for a patent for invention of CN02104889.4 " mechanization seed making method of hybridized rice using three line method "; Proposed a kind of mechanical mixed seeding, mix to have received that to separate hybrid with the look choosing be the three line for hybrid seed production method of selfed seed with recovering: selected a kind of rice varieties of husk colouring discrimination for use in normal husk look; With the inter breed crossing of the normal a kind of paddy rice maintenance line of husk color; Obtain the husk colouring discrimination in the paddy rice maintenance line seed of normal husk look, and then transformation goes out the rice sterile line seed of husk colouring discrimination in normal husk look; With of rice sterile line seed and the husk color normal rice restorer seed machinery mixed seeding of husk colouring discrimination in normal husk look; Flowering stage machinery catch up with powder; It is solid after mechanical mixed receipts husk colouring discrimination is the normal seed of husk color of selfing in the crossbreed seed of normal husk look with recovering; Use the color selector sorting again, obtaining crossbreed seed and recovery respectively is seed.But the method that this patent application proposed mainly is limited to the Three-line Hybrid paddy rice, and uses the normal routine recovery of female fertility system to mix system, and male parent seed content is high in the mixed receipts seed; Obtain the high-purity hybrid seed through expensive color-selection apparatus screening and also have certain degree of difficulty.
More than in existing method and the technology, part has related to Mechanicalized seeding method of hybrid rice, but all has limitation more or less, but still is necessary to grind that wound is a kind of is fit to the method that various breeding of hybrid rice and suitable entire mechanization are produced.
Summary of the invention
The technical problem that the present invention will solve is the deficiency that overcomes prior art; A kind of breeding method that can effectively be applied to mixed seeding, mix the high-frequency recessive female sterile rice restorer lines of receipts formula paddy rice production of hybrid seeds process is provided; A kind of application method of high-frequency recessive female sterile rice restorer lines also is provided; Can realize the Fully-mechanized operation of breeding of hybrid rice, processing through this application, improve the efficient of breeding of hybrid rice greatly.
For solving the problems of the technologies described above; The technical scheme that the present invention proposes is the application of a kind of high-frequency recessive female sterile rice restorer lines in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process; The concrete grammar of said application is: as male parent, as female parent, said father, maternal seed are carried out mixed seeding with the hybrid rice male sterile line with said high-frequency recessive female sterile rice restorer lines; Again the seed after this father, the hybridization of female parent is mixed receipts, accomplish production of hybrid seeds process.
To effectively improve the efficient of hybrid rice production of hybrid seeds process; Alleviate peasant's labour intensity in hybrid rice production of hybrid seeds process; Just must widely popularize the mechanization seeding technique,, then open up wide prospect for applying of the mechanization production of hybrid seeds if can realize the mixed seeding of hybrid rice production of hybrid seeds process, the mixed receipts.Under the prior art condition, realize that the mixed seeding of hybrid rice production of hybrid seeds process mixes receipts mainly based on following technical thought, that is: make father, the special genetic character of maternal increase earlier, handle through follow-up physics or chemical method again, mix the purpose of receiving to reach mixed seeding.And the technical scheme of the invention is the important breakthrough on the prior art basis; We are through using said high-frequency recessive female sterile rice restorer lines; Make the mixed seeding of the whole hybrid rice production of hybrid seeds mix harvest for real; And technical process is simplified greatly; Because we select the male parent material of the production of hybrid seeds for use is the recessive female sterile restoring of high frequency system (especially we are from the recessive female sterile restoring of row filter, the high frequency cultivated system), therefore in the seed that mixes after receiving, only contains the male parent selfed seed of extremely low ratio, mixes removal of impurities that seed after receiving basically need not be follow-up and handle and directly to be used for the land for growing field crops and to produce; And then realize the Fully-mechanized operation of breeding of hybrid rice, processing, improve the efficient of breeding of hybrid rice greatly.
Because the difference of mixed seeding ratio in the technique scheme, perhaps owing to father, the otherness of maternal material on moral character, purity, the purity that might cause mixed seeding to mix receipts back hybrid seed can't satisfy the requirement that the land for growing field crops produces.Based on the technical conceive identical with technique scheme; The present invention also provides another kind of improved technical scheme; Be the application of a kind of high-frequency recessive female sterile rice restorer lines in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process, this is applied on the basis of technique scheme, has further combined existing related techniques; The concrete grammar of said application is: with said high-frequency recessive female sterile rice restorer lines as male parent; And have can screen the genetic specificity shape the hybrid rice male sterile line as female parent, said father, maternal seed are carried out mixed seeding, again the seed after this father, the hybridization of female parent is mixed receipts; Last according to said difference of screening the genetic specificity shape, adopt different separating methods that the seed that mixes after receiving is done further removal of impurities processing.
In the above-mentioned improved technical scheme, the described genetic specificity shape that screens can be any genetic character with differentiation, screening or recognition function, preferably can be a kind of color and luster genetic character with non-common husk color; Under with the situation of hybrid rice male sterile line as female parent with non-common husk color color and luster, follow-up corresponding separating method then is meant the hybrid seed that adopts mechanical look to select separating method to sub-elect to have this color and luster.
In the above-mentioned improved technical scheme, the described genetic specificity shape that screens also preferably is meant to have the antiweed characteristic; Under with the situation of hybrid rice male sterile line as female parent with antiweed characteristic, follow-up corresponding separating method then is meant the spraying herbicide in seedling stage behind the planting seed that mixes after receiving.
Can further improve the purity of mixing the receipts hybrid seed through each technical scheme after the above-mentioned improvement, obtain high-purity hybrid seed near 100%.
In each above-mentioned technical scheme, said high-frequency recessive female sterile rice restorer lines is meant that preferably the female abortion rate of individual plant is at 70%～90% female sterile rice restorer.If the female abortion rate of individual plant is too high, then has influence on the acquisition of this high-frequency recessive female sterile rice restorer lines self seed, and then have influence on the promotion and application of this genetic stocks.If the female abortion rate of individual plant is low excessively, then has influence on mixed seeding and mix the seed purity after receiving, thereby might have influence on the realization of the object of the invention.The female abortion rate of described individual plant is meant preferably that at 70%～90% female sterile rice restorer female sterile restoring is FSV1, is meant that perhaps female sterile restoring is that FSV1 and conventional rice recovery are that the female sterile restoring that obtains after the R hybridization is R
fSaid female sterile restoring is that FSV1 is paddy rice (Oryza sativa) FSV1 that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center; The depositary institution address is a Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica; Preservation date is on October 14th, 2009, and deposit number is CGMCC No.3294.Because through our long-term and arduous ground field experiment and laboratory observation, the female sterile restoring that we screen, cultivate be the female abortion rate of individual plant of FSV1 about 80%, can satisfy the requirement of above-mentioned each technical scheme well.
In each above-mentioned technical scheme, the mixed seeding ratio of said father, maternal seed is preferably 1: (10～40).Because if this mixed seeding ratio is excessive, then there is very big waste in the male parent seed, and the hybrid seed purity that mixes after receiving also can descend thereupon; If this mixed seeding ratio is too small, then the part female parent possibly can not get enough pollen pollinations, has reduced hybrid seed yield, has increased cost.
The present invention also provides a kind of breeding method of above-mentioned high-frequency recessive female sterile rice restorer lines; This method is that to be FSV1 (CGMCC No.3294) recover with conventional rice is R hybridization with above-mentioned female sterile restoring; Obtain the F1 offspring, utilize conventional breeding method from described F1 offspring, to select stabilization characteristics of genetics, strain R that the comprehensive agronomy proterties is good again
f(be that above-mentioned preferred female sterile restoring is R
f), this strain R
fBe the high-frequency recessive female sterile rice restorer lines that makes.Because through us long-term observation and practice, be that FSV1 carries out the production of hybrid seeds as male parent with female sterile restoring directly, it is optimum that combination advantage still can not reach; And adopt the female sterile restoring of newly breeding is R
fAs male parent, because comprehensive agronomy proterties such as its output and coordinate force are that FSV1 has improvement than female sterile restoring all, combination advantage is more obvious.
Compared with prior art; The invention has the advantages that: utilize rice restorer to make male parent with the recessive female sterile hereditary capacity of high frequency; With hybrid rice two be or three be male sterile line as female parent, only contain the male parent selfed seed of extremely low ratio in the seed through mixed seeding, after mix receiving, mix removal of impurities that seed after receiving basically need not be follow-up and handle and can directly be used for the land for growing field crops and produce; And then realize the Fully-mechanized operation of breeding of hybrid rice, processing, improve the efficient of breeding of hybrid rice greatly.The invention has changed in the conventional method with female technical thought of educating recovery system as production of hybrid seeds pollen supplier fully; Replacing that recessive female sterile, the pollen fertility of high frequency recovers normally is the genetic stocks as hybrid seeding; The application that is not merely scale, mechanization, high efficiency seeding technique provides prerequisite, and lays a good foundation for the application of female sterile rice restorer from now on.
The female sterile restoring of using among the present invention is that FSV1 is the paddy rice FSV1 that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); The depositary institution address is a Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica; Preservation date is on October 14th, 2009, and deposit number is CGMCC No.3294.
Description of drawings
Fig. 1 is the process chart of a kind of preferred implementation of the present invention;
Fig. 2 is the embodiment of the invention 1 an application technology flow chart;
Fig. 3 is the embodiment of the invention 2 application technology flow charts;
Fig. 4 is the solid situation photo of FSV1 fringe portion for the female sterile restoring of paddy rice in the specific embodiment of the invention;
Fig. 5 is FSV1 abortion embryo strain (female sterile) situation photo for the female sterile restoring of paddy rice in the specific embodiment of the invention.
Embodiment
The application of the preferred high-frequency recessive female sterile rice restorer lines of a kind of the present invention in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process, its basic procedure is as shown in Figure 1.The concrete grammar of this application is: the female sterile restoring of paddy rice to have the recessive female sterile hereditary capacity of high frequency is that FSV1 is as male parent; With common hybrid rice male sterile line A/S as female parent; To father, maternal seed in 1: the ratio of (10～40) is carried out mixed seeding; Again the seed after this father, the hybridization of female parent is mixed receipts, accomplish production of hybrid seeds process.
The purity of the hybrid seed that mixed receipts obtain after above-mentioned application generally about 98%, can reach the basic demand of secondary seed more than 96% of China's regulation.
Below the preferred embodiment of the invention is done further to introduce.
Embodiment 1:
The application of a kind of high-frequency recessive female sterile rice restorer lines of the present invention in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process, as shown in Figure 2, its concrete steps are following:
1. the recessive female sterile restoring of male parent high frequency is R
fCultivation: the female sterile restoring of paddy rice to have the recessive female sterile hereditary capacity of high frequency is that FSV1 is as male parent; Hybridize with other conventional hybrid rice restoring line R (for example Hunan of Hunan Research Centre for Hybrid Rice's seed selection extensive 299 etc.) or breeding intermediate materials; Obtain the F1 offspring, again with the conventional breeding method from the F1 offspring, select to obtain stabilization characteristics of genetics, the recovery with the female sterile genetic character of high frequency recessiveness that the comprehensive agronomy proterties is good is R
f(R
fSeed can pass through self propagated).
2. maternal three is the acquisition of male sterile line 05Z227A: the paddy rice three with anti-weeding characteristic is that male sterile line 05Z227A introduces from subtropics Agro-ecology research institute of the Chinese Academy of Sciences.This three is to contain weed killer herbicide grass fourth phosphine (Basta) resistance bar gene among the male sterile line 05Z227A.
3. mixed seeding mixes and receives: with the recessive female sterile restoring of the high frequency of above-mentioned improvement is R
f(can be FSV1 with the female sterile restoring of paddy rice directly also) be made male parent; With three is that male sterile line 05Z227A is as female parent; Father, maternal seed fully mix in 1: 25 ratio, adopt conventional method presoaking and germinating, mechanical planting again, and the duration from seeding to heading of maternal seed is than about Zao 3 days of male parent seed (production of hybrid seeds this florescence of father and mother is fully met); After machinery mixes receives, institute obtains the purity about 98% of hybrid seed in the seed that mixes after the receipts.
4, follow-up removal of impurities: the mechanical live mode of production that the seeds using after the above-mentioned mixed receipts is conventional is carried out field seeding; With reference to the method for weed killer herbicide operation instructions at spraying herbicide Basta in seedling stage; Remove the male parent seedling, make the land for growing field crops produce seedling purity near 100%.
Embodiment 2:
The application of a kind of high-frequency recessive female sterile rice restorer lines of the present invention in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process, as shown in Figure 3, its concrete steps are following:
1. the recessive female sterile restoring of male parent high frequency is R
fCultivation: its method and step are with embodiment 1.
2. the seed selection of the purple 867S of maternal purple shell two-line sterile line: purple 32 (Hunan Research Centre for Hybrid Rice's seed selection) breeding intermediate materials of conventional purple rice material and hybrid rice with the control of dominance single-gene are trained short 64S (Hunan Research Centre for Hybrid Rice's cultivation; For sale) hybridize the purple 867S of purple shell two-line sterile line of the husk purple that from generation system thereafter, filters out.
3. mixed seeding mixes and receives: with the recessive female sterile restoring of the high frequency of above-mentioned improvement is R
f(can be FSV1 with the female sterile restoring of paddy rice directly also) be made male parent; As female parent, father, maternal seed fully mix in 1: 10 ratio with the purple 867S of purple shell two-line sterile line, and the duration from seeding to heading of male parent was about 3 days than female parent; To guarantee that this florescence of father and mother fully meets; Adopt conventional method presoaking and germinating, mechanical planting again, after machinery mixes and receives, the purity of the hybrid seed in the seed that mixes after receiving that obtains can be up to 93%.
4, follow-up removal of impurities: select separating method that the hybrid seed of husk purple and normal yellow male parent seed separate through the conventional mechanical look, with the purity that obtains to be used for land for growing field crops production near 100% hybrid seed.
The female sterile restoring of using among above-mentioned each embodiment of paddy rice be FSV1 grind the wound process and key property following:
Spring in 1993 imported big broomcorn millet (Panicum maximum, tool high frequency facultative apomixis characteristic) genomic DNA in the rice restorer osmanthus 99 with the ear stem injection in the Sanya, and the summer in 1993 obtained low solid variant Vg99 in Changsha; Spring in 1994 Sanya plantation Vg99 the 2nd generation colony, ripening rate separates, and normal solid strain (ripening rate is more than 75%), low solid strain (about ripening rate 20%) and extremely low solid strain (ripening rate contains shaky strain below 1%) occur; Eliminate normal solid strain and extremely low solid strain; Obtain the strain system that low fecundity is not separated from the 3rd generation strain system (summer Changsha in 1994) of low solid strain of the 2nd generation; Educate the multigeneration choosing through Nan Fanbei, breeding the female sterile restoring of material paddy rice with low fecundity and other economical character genetic stability is FSV1 (seeing accompanying drawing 4).
Process microscopy and the female sterile restoring of embryo's dissection discovery are that the pollen of FSV1 is normal, about 80% embryo strain abortion (seeing accompanying drawing 5); With this genetic stocks and typical three is male sterile line V20A (Hunan Research Centre for Hybrid Rice's seed selection; For sale), typical two-line sterile line trains short 64S (Hunan Research Centre for Hybrid Rice's seed selection; For sale) and conventional long-grained nonglutinous rice strain etc. hybridize combo; Hybrid F1 is all solid normal, explains that female sterile restoring is that FSV1 has the recovery gene; F2 isolates normal solid strain and low solid strain according to 3: 1 ratio, and low solid strain ripening rate is consistent with FSV1: explain that this material is the recessive female sterile rice of high frequency of recessive single-gene control.
Claims (5)
1. the application of high-frequency recessive female sterile rice restorer lines in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process; The concrete grammar of said application is: with said high-frequency recessive female sterile rice restorer lines as male parent; As female parent, said father, maternal seed are carried out mixed seeding with the hybrid rice male sterile line, the mixed seeding ratio of said father, maternal seed is 1: (10～40); Again the seed after this father, the hybridization of female parent is mixed receipts, accomplish production of hybrid seeds process; Said high-frequency recessive female sterile rice restorer lines is meant that the female abortion rate of individual plant is at 70%～90% female sterile rice restorer; The female abortion rate of said individual plant is meant that at 70%～90% female sterile rice restorer female sterile restoring is FSV1, is meant that perhaps female sterile restoring is that FSV1 and conventional rice recovery are that the female sterile restoring that obtains after the R hybridization is R
fSaid female sterile restoring is that FSV1 is the paddy rice FSV1 that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.3294.
2. the application of high-frequency recessive female sterile rice restorer lines in mixed seeding, mixed receipts formula paddy rice production of hybrid seeds process; The concrete grammar of said application is: with said high-frequency recessive female sterile rice restorer lines as male parent; And have can screen the genetic specificity shape the hybrid rice male sterile line as female parent; Said father, maternal seed are carried out mixed seeding, and the mixed seeding ratio of said father, maternal seed is 1: (10～40), again the seed after this father, the hybridization of female parent is mixed receipts; Last according to said difference of screening the genetic specificity shape, adopt different separating methods that the seed that mixes after receiving is done further removal of impurities processing; Said high-frequency recessive female sterile rice restorer lines is meant that the female abortion rate of individual plant is at 70%～90% female sterile rice restorer; The female abortion rate of said individual plant is meant that at 70%～90% female sterile rice restorer female sterile restoring is FSV1, is meant that perhaps female sterile restoring is that FSV1 and conventional rice recovery are that the female sterile restoring that obtains after the R hybridization is R
fSaid female sterile restoring is that FSV1 is the paddy rice FSV1 that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.3294.
3. application according to claim 2 is characterized in that: the described genetic specificity shape that screens is meant the color and luster with non-common husk color; Said separating method is meant the hybrid seed that adopts mechanical look to select separating method to sub-elect to have said color and luster.
4. application according to claim 2 is characterized in that: the described genetic specificity shape that screens is meant to have the antiweed characteristic; Said separating method is meant the spraying herbicide in seedling stage behind the planting seed that mixes after receiving.
5. the breeding method of a high-frequency recessive female sterile rice restorer lines described in claim 1 or 2; This method is be that FSV1 and conventional rice recover with female sterile restoring is R hybridization; Obtain the F1 offspring, utilize conventional breeding method from described F1 offspring, to select stabilization characteristics of genetics, strain R that the comprehensive agronomy proterties is good again
f, this strain R
fBe the high-frequency recessive female sterile rice restorer lines that makes; Said female sterile restoring is that FSV1 is the paddy rice FSV1 that is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.3294.
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| CN103834684A (en) * | 2013-03-29 | 2014-06-04 | 湖南杂交水稻研究中心 | Method for mechanically producing seed by using female sterile hybrid rice |
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| CN102972285B (en) * | 2012-12-05 | 2014-03-26 | 湖南杂交水稻研究中心 | Breeding method of hybrid crops |
| CN107125129A (en) * | 2017-05-27 | 2017-09-05 | 湖南杂交水稻研究中心 | A kind of mixed method received and separate hybrid seed of the lopsided glume restorer production of hybrid seeds of utilization rice recessive |
| CN112772404B (en) * | 2020-12-31 | 2021-07-27 | 云南农业大学 | Breeding method of rice female nuclear sterility restoring line |
| CN112715349B (en) * | 2020-12-31 | 2021-07-16 | 云南农业大学 | Breeding method of female genic male sterile line of multi-year type rice |
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| CN101697706A (en) | 2010-04-28 |
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