CN102668977A - Method for cultivating seedless eggplant variety - Google Patents
Method for cultivating seedless eggplant variety Download PDFInfo
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- CN102668977A CN102668977A CN2012101925551A CN201210192555A CN102668977A CN 102668977 A CN102668977 A CN 102668977A CN 2012101925551 A CN2012101925551 A CN 2012101925551A CN 201210192555 A CN201210192555 A CN 201210192555A CN 102668977 A CN102668977 A CN 102668977A
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Abstract
The invention relates to the technical field of agriculture, in particular to a method for cultivating a seedless eggplant variety. According to the method, two characters, namely parthenocarpy and male sterility of eggplants are integrated on the same strain to obtain a novel male sterility breeding material with parthenocarpy performance; and the parthenocarpic rate of the material is about 50 percent, and the sterile plant rate and the sterility degree are over 99 percent. Fruits of the variety are seedless or have few seeds in the whole harvest period, and the character can be stably inherited. The hybrid eggplant variety which is naturally seedless or has few seeds in the whole harvest period can be cultivated by using the eggplant breeding material by a heterosis breeding method; and the natural seedless fruit rate of the variety is about 76 percent generally. Eggplant fruits which are seedless or have few seeds can be produced by using the variety.
Description
Technical field
The present invention relates to agricultural technology field, particularly a kind of method of cultivating no seed eggplant kind.
Background technology
The edible multiselect of eggplant is got young tender fruit, lacks seed or does not have the important indicator that seed is a high-quality.Cultivating no seed or lacking seed eggplant kind is the important goal of eggplant breeding research.
Only at pollination and after fertilization, fruit is setting and growth normally, all contains seed in the fruit usually for plant.The phenomenon that plant can develop into normal fruit without pollination and fertilization is called parthenocarpy, and the fruit of parthenocarpy does not normally have seed.
The parthenocarpy of plant can be divided into genotype and artificial induction's type, and wherein genotypical parthenocarpy is divided into obligate parthenocarpy and facultative parthenocarpy again.The obligate parthenocarpy is meant that parthenocarpy property receives gene control and do not receive the influence of environmental change, heredity stably and expressing, and it can only preserve and breed through vegetative method.Facultative parthenocarpy is meant that parthenocarpy property receives gene control equally; But be unfavorable for that pollination and fertilization condition (like low, high temperature etc.) down can parthenocarpies; Under the control environment condition, can normally pollinate again and be fertilized and set seeds, it can preserve and breed through seed.Artificial induction's parthenocarpy is meant with exogenous plant hormones or pollen extract, perhaps stimulate the inducing plant result with incompatible pollen or the like, and its fruit does not normally have seed, but does not have inheritance.The facultative parthenocarpy breeding material of eggplant is being unfavorable for that pollination and fertilization condition (like low temperature etc.) can bear no seed eggplant, but when environmental condition suited, the fruit of bearing had seed.Therefore the eggplant kind cultivated of the facultative parthenocarpy breeding material of single utilization can not be implemented in whole picking time eggplant fruit does not all have seed or few seed.
Present stage, also do not see the relevant report of cultivating no seed eggplant kind method.The main method of on producing, producing at present no seed eggplant is: first method is to utilize conventional eggplant kind, uses heavy dose of exogenous plant hormones at the florescence, realizes flower and fruit protecting, the promotion fruit expands, and generally is no seed in its fruit.But this method is owing to need manual work that every flower is used hormone, and labor cost increases, owing to use a large amount of exogenous hormones, possibly there is the security-hidden trouble that brings because of exogenous hormone in eggplant in addition; And, remain seed-bearing under optimum conditions in the fruit in case do not use hormone.Second method parthenocarpy breeding material capable of using is cultivated no seed eggplant; But just under the environment that is inappropriate for the pollination fertilization, can produce no seed eggplant; In case environment is suitable, in the words fruit of the normal pollination of gynoecium seed is arranged still, do not reach the requirement that all be no seed whole picking time.The third method is to utilize genetic engineering technology; (iaa) wait gene to import in the eggplant kind in growth hormone; Express at the ovary interior orientation, thereby obtain transgenosis parthenocarpy eggplant material, be unwell to adverse environmental factor that eggplant blossoms and bears fruit ability result down at low temperature etc.; And also be no seed in the fruit, equally with second method do not reach the requirement that all is no seed whole picking time.And because transformed variety has potential safety hazard, it is applied and is restricted.
Therefore, the present invention provides a kind of cultivation not have seed eggplant kind, and does not have the method that the seed proterties can genetic stability, has good realistic meaning and application prospect.
Summary of the invention
In view of this, the present invention provides a kind of method of cultivating no seed eggplant kind.This method obtains the proterties of no seed eggplant can genetic stability, and the natural seedless fruit rate of its fruit is generally about 76%.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme:
The invention provides a kind of parthenocarpy method of breeding material of male sterile eggplant of holding concurrently of cultivating, comprise the steps:
Step 1: as female parent, as the male parent sexual hybridization, obtain hybridization F with parthenocarpy eggplant kind with sterile eggplant kind
1Seed;
Step 2: get hybridization F
1Seed plantation and selfing obtain F
2Seed;
Step 3: plantation F
2Seed, choose have sterile proterties plant as female parent, backcross as male parent with parthenocarpy eggplant kind, obtain BC
1F
1Seed;
Step 4: get BC
1F
1Seed plantation and selfing obtain BC
1F
2Seed;
Step 5: plantation BC
1F
2Seed or step 2 obtain F
2Seed, the plant individual plant of selecting to have sterile proterties and parthenocarpy proterties is simultaneously reserved seed for planting, and through the selfing of 7～9 generations, promptly gets.
As preferably, sterile eggplant kind is F16-5-8, F12-1-1 or F13-1-7.
As preferably, parthenocarpy eggplant kind is D-7-1 or D-16-3.
The present invention also provides a kind of method of cultivating no seed eggplant kind, comprises the steps:
Step 1: as female parent, as the male parent sexual hybridization, obtain hybridization F with parthenocarpy eggplant kind with sterile eggplant kind
1Seed;
Step 2: get hybridization F
1Seed plantation and selfing obtain F
2Seed;
Step 3: plantation F
2Seed, choose have sterile proterties plant as female parent, backcross as male parent with parthenocarpy eggplant kind, obtain BC
1F
1Seed;
Step 4: get BC
1F
1Seed plantation and selfing obtain BC
1F
2Seed;
Step 5: plantation BC
1F
2Seed and/or step 2 obtain F
2Seed, the plant individual plant of selecting to have sterile proterties and parthenocarpy proterties is reserved seed for planting, through the selfing of 5～9 generations, the hold concurrently breeding material of male sterile eggplant of acquisition parthenocarpy;
Step 6: get the parthenocarpy breeding material hybridization of male sterile eggplant of holding concurrently, promptly get through screening.
No seed eggplant kind whole picking time of natural no seed or the few seed cultivated can utilize this variety production not have seed or few seed eggplant fruit, and its natural seedless fruit rate is generally about 76%.
Plants male sterility is meant in the hermaphrodite flower, the fertilization of can not normally pollinating of male organs degeneration, deformity or afunction, and phenomenon with seed can not normally bear fruit.The present invention chooses male sterile eggplant kind as female parent, after selfing, obtains F with parthenocarpy eggplant kind as the hybrid seed that the male parent sexual hybridization obtains
2For seed, selfing again after choosing individual plant with sterile proterties and male parent and backcrossing, how reserve seed for planting for individual plant after, cultivate the stable strain system of regularity height, sterility and parthenocarpy property.Sterility and parthenocarpy property to each strain system are identified, cultivate the good target strain system of comprehensive proterties.
As preferably, male sterile eggplant kind is F16-5-8, F12-1-1 or F13-1-7.
As preferably, parthenocarpy eggplant kind is D-7-1 or D-16-3.
The present invention provides a kind of method of cultivating no seed eggplant kind.The facultative parthenocarpy of eggplant and two proterties of male sterile are aggregated in same article fasten, obtain male sterile breeding material, have the performance of producing no seed eggplant whole picking time with facultative parthenocarpy performance.Utilize this type of eggplant breeding material to adopt the method for heterosis breeding; Just can cultivate the natural no seed of the whole fruit picking time eggplant kind of (perhaps lacking seed); Produce no seed (perhaps lacking seed) eggplant fruit; This proterties that obtains no seed eggplant can genetic stability, significant and good prospects for application in eggplant production.
Embodiment
The invention discloses a kind of method of cultivating no seed eggplant kind, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention and application are described through preferred embodiments; The related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
Cultivation provided by the invention does not have in the method for seed eggplant kind, and the sterile eggplant kind F16-5-8 that chooses, F12-1-1, F13-1-7 and parthenocarpy eggplant kind D-7-1, D-16-3 provide by Chongqing Academy of Agricultural Sciences.
Below in conjunction with instance, further set forth the present invention:
Embodiment 1
With male sterile line F16-5-8, F12-1-1, F13-1-7 is female parent; With 2 parthenocarpy strain D-7-1, D-16-3 is the transformation male parent; The method of adopt sexual hybridization, backcrossing and combining with the pedigree breeding; Through the evaluation of 11 years seed selections, economical character and sterility, parthenocarpy property, cultivated 9 good eggplant functional male sterilities of comprehensive proterties parthenocarpy strain system of holding concurrently, concrete seed selection process is following:
1997: making female parent with F16-5-8, F12-1-1, F13-1-7, was that male parent is hybridized with D-7-1, D-16-3 parthenocarpy material, obtained the first familiar generation combination.
1998: plantation F1 hybrid combination and selfing obtained F2 for seed.
1999: F2 was for seed in plantation, from plant colony, identifies, individual plant and the corresponding transformation male parent selecting to have sterile proterties are backcrossed, and obtained BC1F1 generation.The same year, Yunnan added generation acquisition BC1F2 for seed winter.
2000-2003: F2 generation and BC1F2 are for seed in plantation, for the individual plant purifying of reserving seed for planting, cultivate the stable strain of regularity height, sterility and parthenocarpy property and are more than the plant of from its plant colony, selecting to have simultaneously sterility and parthenocarpy property carries out.Sterility, parthenocarpy property and comprehensive proterties to each strain system are identified, cultivate the good target strain system of comprehensive agronomy proterties.
The sterile double parthenocarpy strain that selects; Its main proterties is stable, and the parthenocarpy rate is about 50%, and sterile plant rate and sterile degree are all more than 99%; Its parthenocarpy rate, sterile plant rate and sterile degree investigation result are seen table 1 to table 3, and main plant proterties and economic characters are seen table 4.
Wherein, Because the eggplant material of using is the functional form male sterile; Just flower pesticide can not normal crack; Therefore the sterile degree authentication method is exactly to use hypocotyl to give eggplant parthenocarpy male sterile material (hypocotyl is green white, stealthy proterties) flower in full bloom (the castrating) pollination of holding concurrently as eggplant kind (the hypocotyl purple the is dominance) pollen of purple, and the seed of gathering in the crops is identified.If behind the seed sprouting that obtains, the hypocotyl color is a purple, shows that this is the offspring of pollination hybridization, if hypocotyl is green white, shows it is the parthenocarpy seed that male sterile material pollination self obtains of holding concurrently.The hold concurrently hybrid rate of male sterile material of the parthenocarpy of measuring is exactly the sterile degree of said material.It can weigh the intensity of the sterility of said material, and numerical value shows that more greatly the intensity of sterility is high more.
Botany proterties and economic characters observed result show that target strain growing way all belongs to medium.Cauline leaf is green, and the fruit atropurpureus is long bar-shaped, more than the heavy 500g of aging fruit.Find out relatively that from the sterile double parthenocarpy strain and the female parent that are selected the ripe property of male sterile line has obtained bigger improvement with fruit shape, economical character meets the plantation and the consumption habit of China.
Table 1 parthenocarpy investigation result
The table 2 parthenocarpy male sterile material sterile plant rate investigation result of holding concurrently
| Investigate total strain number | Sterile strain number | Sterile plant rate (%) |
| 258 | 256 | 99.22 |
The table 3 parthenocarpy male sterile material sterile degree investigation result of holding concurrently
The functional male sterile of the table 4 eggplant parthenocarpy property strain main plant proterties application form of holding concurrently
(unit: cm, g)
2004: utilize the male sterile parthenocarpy eggplant strain of holding concurrently to prepare 45 combinations.
2005-2006: observation, comparative test have been carried out in combination, and having filtered out two performances does not have the combination of seed eggplant preferably.
2007-2008: two performances do not have the combination of seed eggplant preferably and carry out regional test, and have carried out the small size production test, declare Chongqing City's kind identification.
2009: " 04-45 ", " 04-51 " obtained Chongqing City variety of crops validation board and assert, named to be " no seed eggplant No. ", " no seed eggplant No. two ".
The no seed eggplant of table 5 new varieties 04-45, the performance of 04-51 Other Main Agronomic Characters
The no seed eggplant of table 6. new varieties 04-45,04-51 seedless fruit application form
| Combination | Investigation strain number | Investigation fruit number | The seedless fruit number | Few seed fruit number | The polyspermous fruit number | No seed rate | Few seed rate | The seediness rate |
| 04-45 | 20 | 100 | 75 | 16 | 9 | 75％ | 16％ | 9％ |
| 04-51 | 20 | 100 | 78 | 14 | 8 | 78％ | 14％ | 8％ |
| March eggplant | 20 | 100 | 0 | 6 | 94 | 0 | 6％ | 94％ |
| 90-11 | 20 | 100 | 0 | 8 | 92 | 0 | 8％ | 92％ |
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (6)
1. cultivate the parthenocarpy method of breeding material of male sterile eggplant of holding concurrently for one kind, it is characterized in that, comprise the steps:
Step 1: as female parent, as the male parent sexual hybridization, obtain hybridization F with parthenocarpy eggplant kind with sterile eggplant kind
1Seed;
Step 2: get said hybridization F
1Seed plantation and selfing obtain F
2Seed;
Step 3: plant said F
2Seed, choose have sterile proterties plant as female parent, backcross as male parent with said parthenocarpy eggplant kind, obtain BC
1F
1Seed;
Step 4: get said BC
1F
1Seed plantation and selfing obtain BC
1F
2Seed;
Step 5: plant said BC
1F
2Seed or step 2 obtain F
2Seed, the plant individual plant of selecting to have sterile proterties and parthenocarpy proterties is simultaneously reserved seed for planting, and, promptly gets for selfing through 7-9.
2. method according to claim 1 is characterized in that, said sterile eggplant kind is F16-5-8, F12-1-1 or F13-1-7.
3. method according to claim 1 is characterized in that, said parthenocarpy eggplant kind is D-7-1 or D-16-3.
4. a method of cultivating no seed eggplant kind is characterized in that, comprises the steps:
Step 1: as female parent, as the male parent sexual hybridization, obtain hybridization F with parthenocarpy eggplant kind with sterile eggplant kind
1Seed;
Step 2: get said hybridization F
1Seed plantation and selfing obtain F
2Seed;
Step 3: plant said F
2Seed, choose have sterile proterties plant as female parent, backcross as male parent with said parthenocarpy eggplant kind, obtain BC
1F
1Seed;
Step 4: get said BC
1F
1Seed plantation and selfing obtain BC
1F
2Seed;
Step 5: plant said BC
1F
2Seed and/or step 2 obtain F
2Seed, the plant individual plant of selecting to have sterile proterties and parthenocarpy proterties is reserved seed for planting, through the selfing of 7～9 generations, the hold concurrently breeding material of male sterile eggplant of acquisition parthenocarpy;
Step 6: get the said parthenocarpy male sterile eggplant breeding material hybridization of holding concurrently, promptly get through screening.
5. method according to claim 4 is characterized in that, said sterile eggplant kind is F16-5-8, F12-1-1 or F13-1-7.
6. method according to claim 4 is characterized in that, said parthenocarpy eggplant kind is D-7-1 or D-16-3.
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| CN2012101925551A CN102668977A (en) | 2012-06-12 | 2012-06-12 | Method for cultivating seedless eggplant variety |
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| CN2012101925551A CN102668977A (en) | 2012-06-12 | 2012-06-12 | Method for cultivating seedless eggplant variety |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105916372A (en) * | 2013-12-12 | 2016-08-31 | 瑞克斯旺种苗集团公司 | Fertilisation independent fruit formation in eggplant |
| CN110800604A (en) * | 2019-10-21 | 2020-02-18 | 福州市蔬菜科学研究所 | Breeding method and application of eggplant parthenocarpic inbred line |
| CN112931191A (en) * | 2021-04-22 | 2021-06-11 | 重庆市农业科学院 | Breeding method of bacterial wilt-resistant male sterile eggplant rootstock |
| CN115517165A (en) * | 2022-10-20 | 2022-12-27 | 福州市蔬菜科学研究所 | Breeding method of unlimited growth type eggplant inbred line |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105916372A (en) * | 2013-12-12 | 2016-08-31 | 瑞克斯旺种苗集团公司 | Fertilisation independent fruit formation in eggplant |
| CN105916372B (en) * | 2013-12-12 | 2019-08-27 | 瑞克斯旺种苗集团公司 | Fruit in eggplant independent of fertilization is formed |
| US10849303B2 (en) | 2013-12-12 | 2020-12-01 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Fertilisation independent fruit formation in eggplant |
| CN110800604A (en) * | 2019-10-21 | 2020-02-18 | 福州市蔬菜科学研究所 | Breeding method and application of eggplant parthenocarpic inbred line |
| CN112931191A (en) * | 2021-04-22 | 2021-06-11 | 重庆市农业科学院 | Breeding method of bacterial wilt-resistant male sterile eggplant rootstock |
| CN115517165A (en) * | 2022-10-20 | 2022-12-27 | 福州市蔬菜科学研究所 | Breeding method of unlimited growth type eggplant inbred line |
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Application publication date: 20120919 |