CN104206266A - Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line - Google Patents
Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line Download PDFInfo
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- CN104206266A CN104206266A CN201410422867.6A CN201410422867A CN104206266A CN 104206266 A CN104206266 A CN 104206266A CN 201410422867 A CN201410422867 A CN 201410422867A CN 104206266 A CN104206266 A CN 104206266A
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Abstract
The invention discloses a method for rapidly screening a restorer of a pepper yellow sprout cytoplasm male sterile line. The method comprises the following steps: in spring, with a pepper yellow sprout CMS sterile line as a female parent and a pepper and capsicum annuum inbred line as a male parent, performing pairing test crossing; harvesting F1 seeds obtained from different test crossing combinations, respectively numbering; performing plug seedling on the test crossed F1 seeds harvested in the current year, using a 72-hole plug tray, randomly taking two plump seeds from the F1 seeds of each test crossing combination, dividing the two plump seeds into two groups with one seed per group, seeding one seed in each hole, and growing for about 20 days at normal temperature after emerging; placing the two groups of seeds in an illumination culture box for being cultured; taking out two groups of pepper seedlings and then managing at normal temperature until flowering; after one pepper plant flowers three flowers, starting identifying the fertility of the pepper; and comparing identifying results of the two groups of pepper combinations, wherein the pepper combination with expressions all according with the condition is the restorer of the pepper yellow sprout cytoplasm male sterile line. Compared with a conventional breeding method, the method has the characteristics of time-saving, space-saving and cost-saving properties.
Description
Technical field
The invention belongs to breeding field, be specifically related to the method for the restorer of screening the yellow cytoplasm male sterility line of capsicum bud.
Background technology
Male sterile restoring line is the strain (kind) that male sterile line can be made to recover normally solid.Restorer Pistil And Stamen is normal, can self-fertility.And can be divided invest male sterile line bear seeds the plant grown up to, and make its fertility restorer normal, and in the proterties such as output, there is obvious hybrid vigour.The height of male sterile restoring line restoring force and the quality of other proterties, directly affect three and tie up to value on heterosis utilization.With male sterile restoring line seed in the sowing of field large area, the plant grown up to both can be solid by pollination, can show stronger advantage in every respect again.In crossbreeding, male sterile line, male sterility maintainer line and male sterile restoring line must support the use.The three series mating that Here it is it has often been said.
Therefore the screening tool of male sterile restoring line is of great significance, and traditional screening process is generally all carried out large Tanaka, and be easily subject to the impact of weather, temperature is uncontrolled, and screening process is longer.
Summary of the invention
The technical problem that the present invention mainly solves is to provide the method for the restorer of the yellow cytoplasm male sterility line of a kind of rapid screening capsicum bud, greatly can shorten screening time, save time, economize ground, province's expense, for solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A method for the restorer of the yellow cytoplasm male sterility line of rapid screening capsicum bud, is characterized in that,
(1) spring is sterile with the yellow CMS of capsicum bud is maternal, is that male parent carries out paired test cross with capsicum/pimento selfing;
(2) gather in the crops different test cross and combine the F1 seed obtained, number respectively;
(3) the test cross F1 seed gathered in the crops then is carried out point disk seedling growing, with 72 hole dishes, each test cross combination F1 seed gets 2 full seeds at random, is divided into 2 groups, often organizes 1, every bunch planting 1, grow about 20 days after emerging under normal temperature state;
(4) 2 groups of seeds are all placed in illumination box to cultivate; The condition of culture of wherein one group of illumination box is set to: illumination: 20 ~ 22 DEG C/12h, dark: 15 ~ 18 DEG C/12h, in another group illumination box, condition of culture is set to: illumination: 26 ~ 28 DEG C/12h, dark: 20 ~ 22 DEG C/12h, respectively to cultivate 17 ~ 25 days with this understanding;
(5) manage, until bloom under 2 groups of Hot Pepper Seedlings taking-ups being placed on normal temperature state;
(6) when a strain capsicum output three spend come after start to identify its fertility; Identify that content is: a. flower opens; B. pollen is had; C.7 bear fruit in sky, described capsicum will meet the record of above-mentioned three kinds of conditions, otherwise eliminates.
(7) contrasted by 2 groups of capsicum combination qualification results, performance all meets the restorer being the yellow cytoplasm male sterility line of this capsicum bud of the 6th step three articles.
The invention has the beneficial effects as follows:
(1) save time, carry out restorer screening by this invention and screen restorer method with tradition, can save time about 200 days.
(2) economize ground, test and carry out in the dish of cave, only need a small amount of soil.
(3) economize expense, save the expense of qualification in autumn, if there is thermosensitive type male sterility line situation, then need spring and autumn to carry out, the present invention's illumination box carries out, and saves the expense of land for growing field crops qualification in autumn.
Embodiment
Embodiment
Spring with S140 ?2A capsicum cytoplasm male sterility line (utilize publication number for CN 103283587 A
The material that denomination of invention selects for " selection of the environmental male sterile line of the yellow capsicum of a kind of bud and mating system ") for maternal, be that male parent carries out paired test cross with 210 capsicums and pimento material, obtain 210 parts of hybrid combination F1 seeds.
Numbered respectively by 210 parts of F1 seeds and be divided into A, B two groups, each test cross combination F1 seed gets 2 full seeds at random, is divided into 2 groups, often organizes 1, every bunch planting 1 sowing 72 hole dish, normal temperature condition managing 20 days after emerging.
A group Hot Pepper Seedling is placed in temperature and is set to illumination: 21 DEG C, dark: 17 DEG C, the illumination box of illumination/dark: 12/12h, the test of B group is placed in temperature and is set as illumination: 27 DEG C, dark: 21 DEG C, in the illumination box of illumination/dark: 12/12h, to cultivate and take out normal management after 20 days.
After going out 3 and spending, start to identify its fertility, qualification content: a. flower opens; B. pollen is had; C.7 bear fruit in sky.Meet the record of above-mentioned three kinds of conditions, otherwise eliminate.
Contrast is with the pimento of spring and autumn at field seeding, and qualification content is identical, and comparing result is in table 1.
Table 1
As can be seen from the table, the sterile restoring based material that A, B of the present invention two groups screens accounts for 24.8%, 24.8% of experiment material, control group be 24.3%, difference is little, but it is 3 ~ 4 months that the present invention screens the time used, control group but needs year, and screening time greatly reduces.
Claims (3)
1. a method for the restorer of the yellow cytoplasm male sterility line of rapid screening capsicum bud, is characterized in that, comprise the steps:
(1) spring is sterile with the yellow CMS of capsicum bud is maternal, is that male parent carries out paired test cross with capsicum and pimento selfing;
(2) gather in the crops different test cross and combine the F1 seed obtained, number respectively;
(3) the test cross F1 seed gathered in the crops then is carried out point disk seedling growing, with 72 hole dishes, each test cross combination F1 seed gets 2 full seeds at random, is divided into 2 groups, often organizes 1, every bunch planting 1, grow about 20 days after emerging under normal temperature state;
(4) 2 groups of seeds are all placed in illumination box to cultivate;
(5) manage, until bloom under 2 groups of Hot Pepper Seedlings taking-ups being placed on normal temperature state;
(6) when a strain capsicum output three spend come after start to identify its fertility;
(7) 2 groups of capsicum combination qualification results are contrasted, show all qualified restorer being the yellow cytoplasm male sterility line of this capsicum bud.
2. the method for the restorer of the yellow cytoplasm male sterility line of rapid screening capsicum bud as claimed in claim 12, it is characterized in that, the condition of culture of step (4) is: be set to by the condition of culture of wherein one group of illumination box: illumination: 20 ~ 22 DEG C/12h, dark: 15 ~ 18 DEG C/12h, in another group illumination box, condition of culture is set to: illumination: 26 ~ 28 DEG C/12h, dark: 20 ~ 22 DEG C/12h, respectively to cultivate 17 ~ 25 days with this understanding.
3. the method for the restorer of the yellow cytoplasm male sterility line of rapid screening capsicum bud as claimed in claim 1 or 2, it is characterized in that, the Fertility identification content described in step (6) is: a. flower opens; B. pollen is had; C.7 bear fruit in sky, described capsicum will meet the record of above-mentioned three kinds of conditions, otherwise eliminates, and the condition described in step (7) meets the qualification content in (6) step exactly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410422867.6A CN104206266A (en) | 2014-08-25 | 2014-08-25 | Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410422867.6A CN104206266A (en) | 2014-08-25 | 2014-08-25 | Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line |
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| CN104206266A true CN104206266A (en) | 2014-12-17 |
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| CN201410422867.6A Pending CN104206266A (en) | 2014-08-25 | 2014-08-25 | Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075855A (en) * | 2015-09-22 | 2015-11-25 | 镇江瑞繁农艺有限公司 | Method for breeding male sterility restoring line of yellow sprout pepper cytoplasm |
| CN105594587A (en) * | 2016-03-22 | 2016-05-25 | 四川省川椒种业科技有限责任公司 | Breeding method of germplasm material of male sterility restoring line of chilies |
| CN109156336A (en) * | 2018-08-20 | 2019-01-08 | 青岛农业大学 | A kind of selection of High color values and slow sweet tea pigment green pepper restorer of fading |
Citations (4)
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| CN1820581A (en) * | 2006-02-10 | 2006-08-23 | 北京市海淀区植物组织培养技术实验室 | Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line |
| WO2010101274A1 (en) * | 2009-03-05 | 2010-09-10 | 横浜植木株式会社 | Seedless capsicum annuum l. and method for producing same |
| CN102090325A (en) * | 2010-12-02 | 2011-06-15 | 贵州省园艺研究所 | Method for transforming cytoplasmic male sterile line, homomaintainer line and same type restoring line of chillies |
| CN103283587A (en) * | 2013-05-29 | 2013-09-11 | 镇江瑞繁农艺有限公司 | Selective breeding and propagating method of ecological type sterile line of yellow-sprout capsicum |
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2014
- 2014-08-25 CN CN201410422867.6A patent/CN104206266A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820581A (en) * | 2006-02-10 | 2006-08-23 | 北京市海淀区植物组织培养技术实验室 | Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line |
| WO2010101274A1 (en) * | 2009-03-05 | 2010-09-10 | 横浜植木株式会社 | Seedless capsicum annuum l. and method for producing same |
| CN102090325A (en) * | 2010-12-02 | 2011-06-15 | 贵州省园艺研究所 | Method for transforming cytoplasmic male sterile line, homomaintainer line and same type restoring line of chillies |
| CN103283587A (en) * | 2013-05-29 | 2013-09-11 | 镇江瑞繁农艺有限公司 | Selective breeding and propagating method of ecological type sterile line of yellow-sprout capsicum |
Non-Patent Citations (2)
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| 沈火林等: "辣椒雄性不育恢复系选育研究", 《中国园艺学会第五届青年学术讨论会论文集》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075855A (en) * | 2015-09-22 | 2015-11-25 | 镇江瑞繁农艺有限公司 | Method for breeding male sterility restoring line of yellow sprout pepper cytoplasm |
| CN105594587A (en) * | 2016-03-22 | 2016-05-25 | 四川省川椒种业科技有限责任公司 | Breeding method of germplasm material of male sterility restoring line of chilies |
| CN109156336A (en) * | 2018-08-20 | 2019-01-08 | 青岛农业大学 | A kind of selection of High color values and slow sweet tea pigment green pepper restorer of fading |
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Application publication date: 20141217 |