CN105594587A - Breeding method of germplasm material of male sterility restoring line of chilies - Google Patents
Breeding method of germplasm material of male sterility restoring line of chilies Download PDFInfo
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- CN105594587A CN105594587A CN201610165842.1A CN201610165842A CN105594587A CN 105594587 A CN105594587 A CN 105594587A CN 201610165842 A CN201610165842 A CN 201610165842A CN 105594587 A CN105594587 A CN 105594587A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The application discloses a breeding method of a germplasm material of a male sterility restoring line of chilies. The breeding method comprises the following steps: planting an F1-generation new chili variety of fascicled pod peppers to obtain F2; planting the F2 and selecting current-generation fertile plants with high yielding ability, good comprehensive properties and strong stress resistance and disease resistance to be subjected to third-generation planting and selfing; carrying out selfing from the fourth generation to the sixth generation by adopting the same method, so as to obtain an F6-generation fertile plant line; carrying out test crossing on the F6-generation fertile plant line and a bred fascicled pod pepper sterile line E4A, planted respectively, to assemble hybrid F1 novel combinations; and carrying out a plant variety comparison test on the hybrid F1 novel combinations to select the combination which has the best restoring, highest yielding ability, strongest stress resisting capability and good quality. A male parent of the hybrid F1 combination is a novel restoring line germplasm resource which is finally obtained, and is a novel breeding material of the fascicled pod peppers.
Description
Technical field
The application belongs to field of plant breeding, specifically, relates to a kind of hot pepper male sterile extensiveBe the selection of germplasm materials again.
Background technology
The hot pepper male sterile three series mating crossbreeding of science and technology developed country starts to walk early, scientific researchBasis is high, and particularly degeneration-resistant, breeding for disease resistance does better than domestic. It is domestic that to carry out capsicum assortedHand over breeding just to start starting 70 years last century, more developed country is half a century in recent years. InstituteWith, domestic in hot pepper germ plasm resource collection innovation, capsicum heterosis, hybrid vigor utilizes, capsicumMale sterility three line mating breeding technology and the pepper breeding of degeneration-resistant, breeding for disease resistance and capsicumAdvanced country compares very large gap. In order to catch up with foreign pepper breeding advanced level,Introduce the hot pepper germ plasm resource (gene) of screening foreign outstanding, by its outstanding germplasm moneySource, particularly hot pepper male sterile restorer material are applied to domestic hot pepper male sterile threeIt on series mating breeding, is object of the present invention.
In hot pepper male sterile three series mating breeding, general employing at present: capsicumSterile line does female parent, and domestic existing hot pepper germ plasm resource does male parent, through test cross,The full recovery of first-filial generation, its male parent is exactly restorer material. Through facts have proved, 100In the existing hot pepper germ plasm resource of part, restorer material occupies ratio generally only 10% left and right,And in 10 parts of restorer materials, be also difficult to find 1 part of outstanding restorer breeding material,Reason thus, has restricted the progress of China's hot pepper male sterile three series mating breeding technology.
Summary of the invention
In view of this, the application, for problems of the prior art, provides a kind of capsicumThe selection of male sterile restoring line germplasm materials, quotes that external male sterility three line is supporting educatesThe capsicum Hybrid becoming carries out separation screening, through inbreeding of more generation, selects stable maleSterile restoring is hot pepper germ plasm resource (gene), with existing capsicum sterile line material hybridization groupBe made into new F1 generation capsicum new varieties.
In order to solve the problems of the technologies described above, the application discloses a kind of hot pepper male sterile restorerThe selection of germplasm materials, comprises the following steps:
1) select the capsicum annum fasciculatum F1 generation capsicum new varieties of clustering to plant, obtain F2, then enterRow plantation F2, selects yielding ability high, and Comprehensive Traits is good, resistance, this generation that disease resistance is strongFertile plant is carried out third generation plantation selfing, adopts same procedure, carries out for the 4th six generations of generation to the certainlyHand over, show that F6 for fertile plant is;
2) by F6 for fertile plant system and clustering of being bred as be not to educate E4A to plant respectively towards skyPlant and carry out test cross, assembly goes out to hybridize F1 and newly combines;
3) new hybridization F1 combination is carried out to variety comparative test, select recover preferably, high yield is the highest,Anti-adversity ability is the strongest, the combination of quality better, and the male parent of this hybridization F1 combination is the present inventionThe final new capsicum annum fasciculatum restorer germ plasm resource of clustering obtaining.
Further, the capsicum annum fasciculatum F1 generation capsicum new varieties of clustering of specifically selecting in the present invention areNo. 3, the Korea S sky.
Compared with prior art, the application can obtain and comprise following technique effect:
Inventor is through facts have proved of the separation innovation to multiple external capsicum varieties for many years,The external capsicum variety majority of introducing utilizes the supporting incubation of male sterility three line. TherefromYielding ability can be isolated high, the restorer hot pepper germ plasm resource (base degeneration-resistant, disease resistance is strongBecause of), for domestic male sterility three line mating breeding, be a kind of innovation pepper breedingThe new way of material.
Separate the front topic of innovation restorer pepper breeding material, must select in the worldThe best Hybrid of spread, and be to utilize the supporting choosing of male sterility three lineEducate and form, just can accurately obtain wherein outstanding restorer germplasm materials (gene),Be applied in the pepper breeding of oneself.
Differentiate whether the combination of introducing is to utilize the supporting seed selection of male sterility three lineForm, need to observe through its F2 of plantation, if do not isolate sterile in F2Strain, illustrates that this combination is to adopt double-line hybrid seed selection to form, but also can continueCreate conventional breeding material (even obtain and have the breeding material that recovers gene), but notBe same as selection of the present invention and seed selection step.
Certainly, arbitrary product of enforcement the application must not necessarily need to reach above-described simultaneouslyAll technique effects.
Brief description of the drawings
Accompanying drawing described herein is used to provide further understanding of the present application, forms the applicationA part, the application's schematic description and description is used for explaining the application, not structureThe application's improper restriction in pairs. In the accompanying drawings:
Fig. 1 is the flow process of the selection of the application's hot pepper male sterile restorer germplasm materialsFigure; Wherein, " F " adds numeral is selfing algebraically, " ♀ " sterile line female parent, and " ♂ " is extensiveBe male parent again, " F1 " is the first-filial generation of new assembly.
Detailed description of the invention
Below cooperation drawings and Examples are described in detail to the application's embodiment, right by thisThe application's realization how application technology means solve technical problem and reach technology effectJourney can fully understand and implement according to this.
Embodiment 1
The present embodiment is the capsicum annum fasciculatum F1 generation capsicum new varieties " sky of clustering that utilizes Korea S to introduceNo. 3 ", plant F1, plant F2 again, in F2, isolate fertile plant and sterile strain,Wherein 2 yielding abilities are high in selection, and Comprehensive Traits is good, resistance, strong (the rear abbreviation choosing of disease resistanceEducate standard) this generation fertile plant, name: F2-1, F2-2; Carry out third generation plantation selfing,In F3 generation, in every strain, select again i.e. 4 strains of 2 strains by seed selection standard, name: F3-1-1,F3-1-2, F3-2-1, F3-2-2; Carry out the 4th generation plantation selfing, by seed selection standard again at F3In generation, in every strain, select i.e. 8 strains of 2 strains, name: F4-1-1-1, F4-1-1-2,F4-1-2-1, F4-1-2-2, F4-2-1-1, F4-2-1-2, F4-2-2-1, F4-2-2-2; Carry outThe plantation selfing of the 5th generation, in F4 generation, selecting 2 strains in every strain again by seed selection standard is 16Individual strain; Name: F5-1-1-1-1, F5-1-1-1-2, F5-1-1-2-1, F5-1-1-2-2,F5-1-2-1-1、F5-1-2-1-2、F5-1-2-2-1、F5-1-2-2-2、F5-2-1-1-1、F5-2-1-1-2、F5-2-1-2-1、F5-2-1-2-2、F5-2-2-1-1、F5-2-2-1-2、F5-2-2-2-1、F5-2-2-2-2，In F5 generation, selects 2 strains to become 32 strains again in every strain, certainly sends to for the 6th generation, names:F6-1-1-1-1-1、F6-1-1-1-1-2、F6-1-1-1-2-1、F6-1-1-1-2-2、F6-1-1-2-1-1、F6-1-1-2-1-2、F6-1-1-2-2-1、F6-1-1-2-2-2、F6-1-2-1-1-1、F6-1-2-1-1-2、F6-1-2-1-2-1、F6-1-2-1-2-2、F6-1-2-2-1-1、F6-1-2-2-1-2、F6-1-2-2-2-1、 F6-1-2-2-2-2、F6-2-1-1-1-1、F6-2-1-1-1-2、F6-2-1-1-2-1、F6-2-1-1-2-2、F6-2-1-2-1-1、F6-2-1-2-1-2、F6-2-1-2-2-1、F6-2-1-2-2-2、F6-2-2-1-1-1、F6-2-2-1-1-2、F6-2-2-1-2-1、F6-2-2-1-2-2、F6-2-2-2-1-1、F6-2-2-2-1-2、F6-2-2-2-2-1, F6-2-2-2-2-2, each strain gene is basicly stable, just can recoverGenetic test.
Embodiment 2
Will be in " No. 3, the sky " isolated F6 generation 32 strains and the court of clustering that has been bred asIt sterile line E4A plants respectively and carries out test cross, and assembly goes out 32 hybridization F1 newly combines, groupClose method and the results are shown in following table 1.
Table 1 test cross combination table
| Sequence number | Combination | Sequence number | Combination | Sequence number | Combination |
| 1 | E4a×F6-1-1-1-1-1 | 12 | E4a×F6-1-2-1-2-2 | 23 | E4a×F6-2-1-2-2-1 |
| 2 | E4a×F6-1-1-1-1-2 | 13 | E4a×F6-1-2-2-1-1 | 24 | E4a×F6-2-1-2-2-2 |
| 3 | E4a×F6-1-1-1-2-1 | 14 | E4a×F6-1-2-2-1-2 | 25 | E4a×F6-2-2-1-1-1 |
| 4 | E4a×F6-1-1-1-2-2 | 15 | E4a×F6-1-2-2-2-1 | 26 | E4a×F6-2-2-1-1-2 |
| 5 | E4a×F6-1-1-2-1-1 | 16 | E4a×F6-1-2-2-2-2 | 27 | E4a×F6-2-2-1-2-1 |
| 6 | E4a×F6-1-1-2-1-2 | 17 | E4a×F6-2-1-1-1-1 | 28 | E4a×F6-2-2-1-2-2 |
| 7 | E4a×F6-1-1-2-2-1 | 18 | E4a×F6-2-1-1-1-2 | 29 | E4a×F6-2-2-2-1-1 |
| 8 | E4a×F6-1-1-2-2-2 | 19 | E4a×F6-2-1-1-2-1 | 30 | E4a×F6-2-2-2-1-2 |
| 9 | E4a×F6-1-2-1-1-1 | 20 | E4a×F6-2-1-1-2-2 | 31 | E4a×F6-2-2-2-2-1 |
| 10 | E4a×F6-1-2-1-1-2 | 21 | E4a×F6-2-1-2-1-1 | 32 | E4a×F6-2-2-2-2-2 |
| 11 | E4a×F6-1-2-1-2-1 | 22 | E4a×F6-2-1-2-1-2 |
32 cross combinations are carried out to variety comparative test, and test method is as follows: select equal soil fertility,Fertilising, Shi Shui, formulation rate are identical, and management method is consistent, 0.03 mu of every combination cultivated area,Plant seedling 96 strains. Repeat random alignment for three times. Observe at ordinary times its growing way, disease resistance and degeneration-resistantProperty, observe its pollen situation florescence, make a record, after fruit maturation, disposable harvesting is surveyed and is producedAmount, finally select recover preferably, high yield is the highest, anti-adversity ability is the strongest, the group of quality betterClose 1-2, the male parent of this 1-2 hybridization F1 combination is final obtain new extensive of the present inventionBe germ plasm resource (gene) again---the new breeding material of the capsicum annum fasciculatum of clustering.
Use above-mentioned inventive method, inventor has successfully selected dissimilar capsicum restorer and has educatedBecome material several, and be applied in multiple Hybrid capsicum breeding of new varieties.
Some preferred embodiments of invention are shown and have been described in above-mentioned explanation, but as previously mentioned, shouldBe not limited to disclosed form herein when understanding invention, should do not regard as other embodimentEliminating, and can be used for various other combinations, amendment and environment, and can be at described hereinIn bright contemplated scope, change by technology or the knowledge of above-mentioned instruction or association area. AndThe change that those skilled in the art carry out and change and do not depart from the spirit and scope of invention, all should beIn the protection domain of invention claims.
Claims (2)
1. a selection for hot pepper male sterile restorer germplasm materials, is characterized in that, comprise withLower step:
1) select the capsicum annum fasciculatum F1 generation capsicum new varieties of clustering to plant, obtain F2, then plant F2,Select yielding ability high, Comprehensive Traits is good, and resistance, this generation fertile plant that disease resistance is strong are carried out third generation kindPlant selfing, adopt same procedure, carry out the six generations selfing of the 4th generation to the, show that F6 for fertile plant is;
2) by F6 for fertile plant system and clustering of being bred as be not to educate E4A to plant and survey respectively towards skyHand over, assembly goes out to hybridize F1 and newly combines;
3) new hybridization F1 combination is planted to variety comparative test, select recover preferably, high yield is the highest,Anti-adversity ability is the strongest, the combination of quality better, and the male parent of this hybridization F1 combination is the final acquisition of the present inventionNew restorer germ plasm resource, the new breeding material of the capsicum annum fasciculatum of clustering.
2. the selection of hot pepper male sterile restorer germplasm materials according to claim 1, itsBe characterised in that, described in the capsicum annum fasciculatum F1 generation capsicum new varieties of clustering specifically select the sky No. 3.
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