CN107135941B - A kind of method of the surging three systems combination of quick breeding cotton - Google Patents
A kind of method of the surging three systems combination of quick breeding cotton Download PDFInfo
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- CN107135941B CN107135941B CN201710613189.5A CN201710613189A CN107135941B CN 107135941 B CN107135941 B CN 107135941B CN 201710613189 A CN201710613189 A CN 201710613189A CN 107135941 B CN107135941 B CN 107135941B
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- restorer
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method of the surging three systems combination of quick breeding cotton, the germ plasm resource by selecting Comprehensive Traits excellent is hybridized with restorer as parent, is compared by multiple years advantage, filters out the advantageous combination F of yield and quality1；Reselection F1Correspondence parent as male parent, hybridize with sterile line, obtain hybridization first filial generation；Then F is selected1Correspondence parent be returned to obtain BC as male parent, then with first filial generation is hybridized1F1；BC1F1For female parent, then and be returned with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource, after backcrossing mostly generation, filter out sterile plant, obtain BCnF1, from BCnF1In filter out with the sterile line that restorer coordinate force is higher, Comprehensive Traits are excellent, then hybridize with restorer and be measured, by plant comparative test, obtain cotton advantage " combination of three systems " of the present invention.
Description
Technical field
The present invention relates to cotton variety breeding technique fields, and in particular to a kind of combination of three systems of quick breeding cotton advantage
Method.
Background technique
Cotton has significant hybrid vigour.It is to improve output of cotton, improving quality, enhancing resistance using hybrid vigour
One of important channel.In recent years some practical reasons cause China hybrid cotton to develop slowly, but hybrid cotton cultivated area still has
1005.8 ten thousand mu, total cotton plants area 16.5% is accounted for, and is mainly planted in Yangtze river basin cotton region, this illustrates hybrid cotton industry also
Certain development prospect.
Cotton three series cenospecies has many advantages, such as that saving of labor, seed purity is high, cost is relatively low, easily forms industrialization, is to realize
Maximally efficient one of the approach of cotton hybrid vigor benefit.Cotton three series refer to sterile line, keep system, restorer.
At present restoring line of cotton change educate be hybridize, reestablish diplomatic relations or be returned with existing restorer with other parents, and
From F2Start, per generation carries out recovery capability identification and screening, to obtain novel restorer.This mode is long there are the period,
The problems such as heavy workload；Meanwhile the breeding of cotton sterile line and restorer is to separate progress, leads to specific combining ability between the two
Difference, although realizing three series mating, the cross combination advantage made is unobvious, it is difficult to apply in production.
Summary of the invention
In view of the deficiency of the prior art, the object of the present invention is to provide one kind to pass through directional transformation sterile line,
The method of the surging three systems combination of quick breeding cotton.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of surging three systems combination of quick breeding cotton
Method includes the following steps:
(1) germ plasm resource for selecting Comprehensive Traits excellent is hybridized as parent with restorer, excellent by multiple years
Gesture compares, and filters out the advantageous combination F of yield and quality1；
(2) F in step (1) is selected1Correspondence parent, i.e., with restorer coordinate force is higher, Comprehensive Traits are excellent germplasm
Resource is hybridized as male parent, then with sterile line as female parent, and hybridization first filial generation is obtained；
(3) F in step (1) is selected1Correspondence parent, i.e., with restorer coordinate force is higher, Comprehensive Traits are excellent germplasm
Resource carries out modification backcrossing as female parent as male parent, then with the first filial generation that hybridizes in step (2), obtains BC1F1；
(4) BC is used1F1For female parent, then and with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource as father
This is returned, and second backcross generation BC is obtained2F1, after continuing backcrossing mostly generation, sterile plant is filtered out, BC is obtainednF1,；
(5) from BCnF1In filter out with the sterile line that restorer coordinate force is higher, Comprehensive Traits are excellent, then it is miscellaneous with restorer
Friendship is measured, and by plant comparative test, it is surging " combination of three systems " to obtain cotton of the present invention.
The method of screening sterile line is by BC in step (5)nF1Hybridized with restorer, filters out yield and quality
Advantageous combination F1, then advantageous combination F1Corresponding parent as provides with restorer coordinate force is higher, Comprehensive Traits are excellent germplasm
Source is as male parent.
Compared with prior art, the advantages of present invention has and good effect:
The present invention is filtered out and restoring line of cotton is special matches in the germ plasm resource excellent from Comprehensive Traits by being measured extensively
Higher parent with joint efforts, and be to keep system with the parent, by hybridizing and be returned, obtain with restoring line of cotton specific combining ability compared with
High sterile line, and prepare surging " combination of three systems ".Method of the invention effectively avoid per generation be required to restoring force test and
The complicated transformation process of time-consuming restorer is transferred to the transformation to restorer and utilizes the breeding with restorer high-combining ability
Material filters out the probability of three system's advantages combination to that can greatly improve in the transformation of sterile line, reduces the blindness of three-line breeding
Property.
Detailed description of the invention
Fig. 1 is the flow chart of selection of the present invention.
Specific embodiment
Now in conjunction with specific embodiment, next the invention will be further elaborated.Specific embodiment described herein is only
It is used to explain the present invention, is not intended to limit the present invention.Test method without specific conditions in following embodiments, according to normal
Method and condition is advised, or is selected according to product manual.
As shown in Figure 1, the method for the surging three systems combination sterile line of quick breeding cotton, includes the following steps:
(1) germ plasm resource for selecting Comprehensive Traits excellent is hybridized as parent with restorer, excellent by multiple years
Gesture compares, and filters out the advantageous combination F of yield and quality1.Comprehensive Traits are primarily referred to as high ginning outturn, big bell, high yield, high-quality, anti-
The characters such as worm, disease-resistant, degeneration-resistant.The excellent germ plasm resource source of Comprehensive Traits: it is a, artificial reconstructed, by distant hybridization, backcrossing, turn
The means such as gene, the excellent New idioplasm resource of initiative Comprehensive Traits；B, resource is introduced and is collected, and it is excellent to introduce other regional representations
Germ plasm resource and collect the high-quality resource that locally makes a variation.
(2) F in step (1) is selected1Correspondence parent, i.e., with restorer coordinate force is higher, Comprehensive Traits are excellent germplasm
Resource is hybridized as male parent, then with sterile line as female parent, and hybridization first filial generation is obtained.
(3) F in step (1) is selected1Correspondence parent, i.e., with restorer coordinate force is higher, Comprehensive Traits are excellent germplasm
Resource carries out modification backcrossing as female parent as male parent, then with the first filial generation that hybridizes in step (2), obtains BC1F1；Because not being institute
Some germ plasm resource is all higher with restorer coordinate force, but is measured by extensive (a large amount of), can screen and cooperate with restorer
The higher resource of power.
(4) BC is used1F1For female parent, then and with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource as father
This is returned, and second backcross generation BC is obtained2F1, after continuing backcrossing mostly generation, sterile plant is filtered out, BC is obtainednF1,；
(5) from BCnF1In filter out with the sterile line that restorer coordinate force is higher, Comprehensive Traits are excellent, then it is miscellaneous with restorer
Friendship is measured, and by plant comparative test, it is surging " combination of three systems " to obtain cotton of the present invention.
Embodiment one
2009, Changde using 49 parts of materials such as the excellent Hunan C1001-, the germ plasm resource Hunan C1049 of Comprehensive Traits and was restored
It is that 61R-9 is measured.
The winter -2010 years 2009, the ground such as Sanya, Changde, Yueyang found (Hunan C1027 × 61 by plant comparative test
R-9) F1It puts up the best performance (yield and quality).
Winter in 2010, Sanya are female parent with sterile line 9A, and using Hunan C1027 as male parent, hybridization is obtained (9A × Hunan C1027)
F1。
2011, Changde, with (9A × Hunan C1027) F1For female parent, Hunan C1027 is male parent, and backcrossing obtains (9A × Hunan
C1027) BC1F1。
The winter -2015 years 2011, in Changde and Sanya two places annual two season, continue to be returned, obtain (9A × Hunan C1027)
BC9F1, and sterile plant is screened, per generation shows single plant according to various trait and receives flower, obtains multiple strains.
Winter in 2015, Sanya, from (9A × Hunan C1027) BC9F1Middle screening simultaneously obtains Hunan C1027-1, Hunan C1027-13 etc. 19
The stable sterile line of part.And it is measured with restorer 61R-9.
2016, Changde found (Hunan C1027-13 × 61R-9) F by plant comparative test1Yield first.It obtains not
Educate based material Hunan C1027-13 and advantage " combination of three systems " Hunan C1027-13 × 61R-9.
Claims (2)
1. a kind of method of the surging three systems combination of quick breeding cotton, includes the following steps:
(1) germ plasm resource for selecting Comprehensive Traits excellent is hybridized as parent with restorer, by multiple years odds ratio
Compared with filtering out the advantageous combination F of yield and quality1；
(2) F in step (1) is selected1Correspondence parent, i.e., make with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource
For male parent, then hybridized as female parent with sterile line, obtains hybridization first filial generation；
(3) F in step (1) is selected1Correspondence parent, i.e., make with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource
For male parent, then with the first filial generation that hybridizes in step (2) modification backcrossing is carried out as female parent, obtains BC1F1；
(4) BC is used1F1For female parent, then and with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource as male parent into
Row backcrossing, obtains second backcross generation BC2F1, after continuing backcrossing mostly generation, sterile plant is filtered out, BC is obtainednF1,；
(5) from BCnF1In filter out with the sterile line that restorer coordinate force is higher, Comprehensive Traits are excellent, then hybridize survey with restorer
Match, by plant comparative test, it is surging " combination of three systems " to obtain cotton of the present invention.
2. a kind of method of the surging three systems combination of quick breeding cotton according to claim 1, the middle screening of step (5) is not
Educating the method for being is by BCnF1Hybridized with restorer, filters out the advantageous combination F of yield and quality1, then advantageous combination F1
Corresponding parent, as with restorer coordinate force is higher, Comprehensive Traits are excellent germ plasm resource.
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| CN201710613189.5A CN107135941B (en) | 2017-07-25 | 2017-07-25 | A kind of method of the surging three systems combination of quick breeding cotton |
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| CN107135941B true CN107135941B (en) | 2019-02-12 |
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| CN110352847B (en) * | 2019-08-16 | 2022-03-11 | 湖南省棉花科学研究所 | Rapid breeding and combined testing and matching identification method for cotton nuclear male sterile line |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598083A (en) * | 2013-09-11 | 2014-02-26 | 山西省农业科学院棉花研究所 | Method of synchronously breeding cotton restorer line and sterile line |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598083A (en) * | 2013-09-11 | 2014-02-26 | 山西省农业科学院棉花研究所 | Method of synchronously breeding cotton restorer line and sterile line |
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