CN108338070A - A kind of method of selection and breeding sorghum homogeneity restorer - Google Patents

A kind of method of selection and breeding sorghum homogeneity restorer Download PDF

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Publication number
CN108338070A
CN108338070A CN201810160598.9A CN201810160598A CN108338070A CN 108338070 A CN108338070 A CN 108338070A CN 201810160598 A CN201810160598 A CN 201810160598A CN 108338070 A CN108338070 A CN 108338070A
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China
Prior art keywords
restorer
selection
homogeneity
breeding
sorghum
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CN201810160598.9A
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Chinese (zh)
Inventor
高明超
李继洪
侯佳明
陈冰嬬
杨微
王阳
高士杰
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Jilin Academy of Agricultural Sciences
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Jilin Academy of Agricultural Sciences
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Priority to CN201810160598.9A priority Critical patent/CN108338070A/en
Publication of CN108338070A publication Critical patent/CN108338070A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of methods of selection and breeding sorghum homogeneity restorer, the narrow characteristic of leaf of short stem of the mainly restoring gene and sterile line of application recovery system carries out artificial hybridization, that is the hybridizing method selection and breeding Poa linn of short stem of A × R, remove artificial emasculation from, it is identical as the cytoplasm of sterile line, so being homogeneity restorer.And expand resource application range.The major advantage for the restorer Ji R125 that the present invention formulates out is that plant is short, plant height at 100 centimetres hereinafter, prepare cenospecies height at 120 centimetres hereinafter, the suitable for mechanization of resistance to dense planting of short stem produce.And resource application range is expanded, it will regenerate and play a role in promoting to sorghum precocity area of China Cultivar replacing.

Description

A kind of method of selection and breeding sorghum homogeneity restorer
Technical field
The present invention relates to sorghum breeding technique field more particularly to a kind of methods of selection and breeding sorghum homogeneity restorer.
Background technology
What is applied in China's sorghum production is cenospecies.Therefore, initiative new parent system is the premise for cultivating the hybrid strain with high yield. What is applied in being produced before last century the eighties is that high stalk hybrid sorghum weaves while producing grain using stalk Frame material etc., with the development and social progress of chemical industry, the purposes of stalk is reduced;Therefore sorghum production turns after the nineties For the middle stalk the hybrid strain with high yield suitable for manually harvesting;With artificial rise in price, production must mechanization reduce production cost. In order to adapt to mechanization growth requirement, it is necessary to cultivate parent and its cenospecies of suitable for mechanization of short stem.Therefore, the present invention will train Parent material of short stem is educated as main breeding objective, and this item work is hybridized to cultivate recovery of short stem with restorer using sterile line System.
Sorghum hybrid application is three systems, i.e. sterile line, maintainer and restorer, and some maintenance materials are prepared miscellaneous Kind is handed over to do well, since the cenospecies matched is unable to self-fertility, so may not apply to produce, if this excellent guarantor Production can be directly applied at recovery-type material by holding the material transformation of type, not only solve the problems, such as fertility in this way, but also solve Problem of short stem.The present invention cultivates the serial restorer such as lucky R125 of precocious wide adaptability of short stem by the effort of more than ten years.
Invention content
Technical problems based on background technology, the present invention propose a kind of method of selection and breeding sorghum homogeneity restorer.
Technical scheme is as follows:
A kind of method of selection and breeding sorghum homogeneity restorer, includes the following steps:
The first step:First × second, obtains F1, wherein first is female parent, and second is male parent;
Second step:First × F1, and Precocious Strains of short stem is continuously selected to be returned, the BCx that obtains BC1, BC2, BC3 successively ... ..., until Obtain the backcross progeny BCx alike with former sterile line first character;
Third walks:BCx is selfed and is purified, obtains BCxF successively1、BCxF2、BCxF3... ... BCxFy, until BCxFyCharacter It is basicly stable;
4th step:BCxFySelfing purifying, obtains the restorer of character stabilization;
In the first step, first is excellent sterile system, second is Elite restorer line.
Preferably, the first is excellent sterile system Ji 1230A, and second is Elite restorer line Ji R105.
Preferably, the restorer of short stem for the stabilization that the present invention obtains is Ji R125, and 100 centimetres of plant height, blade is narrow, plant type It is good, 110 days breeding times;Seedling green, 26 centimetres of spike length, the red red grain of shell, coordinate force are high.
Preferably, the first can also be excellent maintainer.
Preferably, method of the invention can be by arbitrary sterile line or maintainer transformation at restorer.
The invention has the beneficial effects that:
The present invention is mainly that the narrow characteristic of leaf of short stem of the restoring gene and sterile line of application recovery system carries out artificial hybridization, i.e., The hybridizing method selection and breeding Poa linn of short stem of A × R, removes artificial emasculation from, identical as the cytoplasm of sterile line, so being homogeneity Restorer.And expand resource application range.
The major advantage for the restorer Ji R125 that the present invention formulates out is that plant is short, and plant height is at 100 centimetres hereinafter, matching The cenospecies height of system is at 120 centimetres hereinafter, the suitable for mechanization of resistance to dense planting of short stem produces.And resource application range is expanded, It will regenerate and play a role in promoting to sorghum precocity area of China Cultivar replacing.
Description of the drawings
Fig. 1:In embodiment 1, sorghum restorer Ji R125 Breeding Process figures of short stem.
Specific implementation mode
Embodiment 1:
A kind of method of selection and breeding sorghum homogeneity restorer, includes the following steps:
The first step:First × second, obtains F1, wherein first is female parent, and second is male parent;
Second step:First × F1, and Precocious Strains of short stem is continuously selected to be returned, BC1, BC2, BC3 are obtained successively;BC3 and original infertility It is that first character is alike;
Third walks:BC3 is selfed and is purified, obtains BC3F successively1、BC3F2、BC3F3、BC3F4;BC3F4Character is basicly stable;
4th step:BC3F4Selfing purifying, obtains the restorer Ji R125 of character stabilization;
The first is excellent sterile system Ji 1230A, and second is Elite restorer line Ji R105.
The restorer of short stem for the stabilization that the present invention obtains is Ji R125, and 100 centimetres of plant height, blade is narrow, and plant type is good, breeding time 110 days;Seedling green, 26 centimetres of spike length, the red red grain of shell, coordinate force are high.
Embodiment 2:
First in embodiment 1 is replaced with into excellent maintainer, remaining method is constant.
Comparative example
The Chinese invention patent CN107517846A of Sorghum Research Institute, Shanxi Academy of Agricultural Sciences's application (is hereinafter referred to as compared Example) provide it is a kind of using sterile line as the method for recurrent parent selection and breeding A3 restorers.
The difference of the embodiment of the present invention and comparative example:
The selection-breeding method that this method provides and the method for the present invention are more similar, but still have following difference:
First, material therefor is different with the purpose of selection:The present invention's is the restorer restored to A1 and A2 types, comparison Example is A3 types restorer (A3 type restorers are considerably less, so comparative example uses extensive Crossing system);Therefore the present invention selects It is the qualitative selection for having target to select, and comparative example is to be widely separated middle selection after hybridization.
Second is that method is different:In the present invention, be directly returned in first familiar generation and original sterile line, backcross progeny continue with Original sterile line backcrossing, offspring to be returned and sterile line are alike to be selfed again, selects new extensive similar with sterile line of form Multiple system.And in comparative example, sterile line hybridizes with A3 restorers, F1 generation selfing, the loose powder strain self cross and selection separated from F2, warp It crosses multiple self cross and selection and goes out new restorer line;Also it can continue to be selfed and be returned in segregating generation selection loose powder strain and the sterile plant separated It hands over, then selects new restorer line through inbreeding of more generation.
Third, result difference to get to restorer character be different:What the present invention obtained is to have short and small sterile line The restorer of form is conducive to solve suitable for mechanization short-stalked variety select permeability;It is asked the other is solving resource and utilizing extensively It inscribes, such as the cenospecies that the material having is configured to is fine, but does not restore shaky, may not apply to produce, so this kind of Based material transformation is kept just to solve the problems, such as this at restorer.Therefore, the method for the present invention can be used arbitrary sterile line or Person's maintainer transformation is at restorer.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (5)

1. a kind of method of selection and breeding sorghum homogeneity restorer, which is characterized in that include the following steps:
The first step:First × second, obtains F1, wherein first is female parent, and second is male parent;
Second step:First × F1, and Precocious Strains of short stem is continuously selected to be returned, the BCx that obtains BC1, BC2, BC3 successively ... ..., until obtaining The alike backcross progeny BCx with former sterile line first character;
Third walks:BCx is selfed and is purified, obtains BCxF successively1、BCxF2、BCxF3... ... BCxFy, until BCxFyCharacter is basic Stablize;
4th step:BCxFySelfing purifying, obtains the restorer of character stabilization;
In the first step, first is excellent sterile system, second is Elite restorer line.
2. the method for selection and breeding sorghum homogeneity restorer as described in claim 1, which is characterized in that the first is excellent sterile It is Ji 1230A, second is Elite restorer line Ji R105.
3. the method for selection and breeding sorghum homogeneity restorer as claimed in claim 2, which is characterized in that the stabilization that the present invention obtains Restorer of short stem is Ji R125, and 100 centimetres of plant height, blade is narrow, and plant type is good, 110 days breeding times;Seedling green, 26 centimetres of spike length, The red red grain of shell, coordinate force are high.
4. the method for selection and breeding sorghum homogeneity restorer as described in claim 1, which is characterized in that the first can also be excellent Good maintainer.
5. the method for selection and breeding sorghum homogeneity restorer as described in claim 1, which is characterized in that this method can be by arbitrary infertility System or maintainer transformation are at restorer.
CN201810160598.9A 2018-02-26 2018-02-26 A kind of method of selection and breeding sorghum homogeneity restorer Pending CN108338070A (en)

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CN201810160598.9A CN108338070A (en) 2018-02-26 2018-02-26 A kind of method of selection and breeding sorghum homogeneity restorer

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109479708A (en) * 2019-01-24 2019-03-19 吉林省农业科学院 Sorghum keeps being method and restorer Ji BR115 of the transformation at restorer
CN115226625A (en) * 2022-07-18 2022-10-25 商丘市农林科学院 Breeding method of sorghum restorer line

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521735A (en) * 2014-11-28 2015-04-22 山西省农业科学院高粱研究所 Method for creating sorghum A3 cytoplasmic male sterility restorer line
CN105210855A (en) * 2015-10-29 2016-01-06 吉林省农业科学院 A kind of method of formulating sorghum restorer line R109
CN105961192A (en) * 2016-06-07 2016-09-28 吉林省农业科学院 Method for breeding sorghum sterile line through stabilizing and backcrossing method
CN107517846A (en) * 2017-10-25 2017-12-29 山西省农业科学院高粱研究所 A kind of method using sterile line as recurrent parent seed selection A3 restorers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104521735A (en) * 2014-11-28 2015-04-22 山西省农业科学院高粱研究所 Method for creating sorghum A3 cytoplasmic male sterility restorer line
CN105210855A (en) * 2015-10-29 2016-01-06 吉林省农业科学院 A kind of method of formulating sorghum restorer line R109
CN105961192A (en) * 2016-06-07 2016-09-28 吉林省农业科学院 Method for breeding sorghum sterile line through stabilizing and backcrossing method
CN107517846A (en) * 2017-10-25 2017-12-29 山西省农业科学院高粱研究所 A kind of method using sterile line as recurrent parent seed selection A3 restorers

Non-Patent Citations (2)

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Title
卢庆善等: ""高粱恢复系遗传改良策略和进展"", 《辽宁农业科学》 *
李继洪等: ""矮秆_极早熟高粱杂交种吉杂140的选育与栽培研究"", 《园艺与种苗》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109479708A (en) * 2019-01-24 2019-03-19 吉林省农业科学院 Sorghum keeps being method and restorer Ji BR115 of the transformation at restorer
CN115226625A (en) * 2022-07-18 2022-10-25 商丘市农林科学院 Breeding method of sorghum restorer line

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Application publication date: 20180731