CN107517846A - A kind of method using sterile line as recurrent parent seed selection A3 restorers - Google Patents
A kind of method using sterile line as recurrent parent seed selection A3 restorers Download PDFInfo
- Publication number
- CN107517846A CN107517846A CN201711014440.2A CN201711014440A CN107517846A CN 107517846 A CN107517846 A CN 107517846A CN 201711014440 A CN201711014440 A CN 201711014440A CN 107517846 A CN107517846 A CN 107517846A
- Authority
- CN
- China
- Prior art keywords
- selfed
- restorers
- seed selection
- grade
- selection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a kind of method using sterile line as recurrent parent seed selection A3 restorers, including selection cross method and backcross breeding method, described selection cross method to comprise the following steps：The first step：A-grade in the first class 3A × second, without emasculation, pollinated directly to A；Second step：F1 is selfed；3rd step：F2 is selfed, and selects that fecundity is good, character fine individual plant；4th step：F3 selfing purifying, selects that fecundity is good, character fine individual plant；5th step：Character is basicly stable, due to being the seed selection under A3 cytoplasm backgrounds, so restoring force is strong；Wherein, second is A3 recovery resources.Method sequencing provided by the invention is simple, and workload is small, and the time limit is short, efficiency high.
Description
Technical field
The present invention relates to a kind of method using sterile line as recurrent parent seed selection A3 restorers.
Background technology
Sorghum A3 cytoplasmic male sterile lines are that J. V. Worstell, H. J. Kidd and Schertz are equal to 1980
It is bred as, the beginning of the eighties in last century introduces China.Its cytoplasm（IS1112C）From all drawings-double-colored race（Durra—
bicolor race）The bald group of all drawing-dipped beams（Durra—Subglabrescens group）, originate from India.In all moneys
In source material, only minimal amount of material can recover to its sterility, and substantial amounts of test cross shows, almost all of CHINA SORGHUM money
Source material can not all recover its fertility, all be its good holding source.But A3 cytoplasmic male sterile lines have multiple advantage, with
It is the application production of the maternal Sorghum Hybrid Grass prepared, but also fails to commodity using it as the grain sorghum cenospecies that female parent is prepared
Metaplasia is produced, and some applications are simply developed in breeding research.
1. the Testers for A1, A2 maintainer seed selection
Utilization about A3 cytoplasmic male sterile lines, we just proposed in 1987 A3 types cytoplasmic male sterile line can be used for A1,
The Testers of A2 maintainer seed selections.The seed selection of each sterile line, will through and assembling combination, separation, selection, treat that seed selection material is steady
After fixed, then backcross breeding work is carried out, transformation 3-4 can be just measured after generation, be eliminated according to coordinate force, workload is very
Greatly.If the restorer transformation being measured into A3 sterile lines, so that it may straight with the holding material of seed selection before backcross transformation
Connect and be measured, before superseded work is made in backcross transformation, so can largely reduce test area and workload, while accelerate and educate
Kind process, improves Selection effect.
Roderick D. Lee etc.（1993）, Zou Jianqiu etc.（1995）, Chen Yue etc.（1998）All research is had shown that in maintainer
Be returned before sterilization, method of the cytoplasmic test system of apparatus A3 types as the excellent maintainer of screening, be it is practicable,
There is huge application potential in breeding work.
In the Breeding Process of A1, A2 type male sterile line, there is advantages below with the method：1. can early generation test B systems
Coordinate force and hybrid vigour；It is returned afterwards 2. first eliminating, purpose is strong；3. it can therefore save a large amount of human and material resources, financial resources；④
Accelerate breeding process, avoid beneficial gene from producing genetic shift in long Breeding Process.
2. used in population improvement
To in the cytoplasmic researchs of A3 it has been found that it also has larger purposes in population improvement.Using interaction recurrent selection
During method, the coordinate force of B unexpected mass incident materials is often surveyed with the material in R colonies, because the character of two materials is all unstable
Fixed, the coordinate force to F1 is difficult accurate judgement.If main parent's transformations of composition R colonies into A3 sterile lines, as B colonies
Testers, the Selection effects of B colonies can be effectively improved.
Chen Yue, Shi Yuxue（1991）It is also proposed that A3 cell types matter can be applied in colony's recurrent selection.
3. grain sorghum is breeding hybridized
Chen Yue etc.（1994）Test cross is carried out with A1, A2 and A3 cytoplasmic male sterile line using sorghum conversion system, is studied thin to 3 kinds
The Fertility reaction of kytoplasm.As a result show, SC399-14E, SC499-14E can recover the cytoplasmic sterility of A3.
Hou Heting etc.（2000）Report, have found the restorer 1174 of A3 sterile lines（GD55546）, recovery rate 60%~
95%.
We（2000）It has studied fertility table of A3, A4,9E cytoplasmic male sterile line from different sorghum strain test cross F1
It is existing, there are 4 parts of external sorghum strain IS1112C, IS4988C, SC240-14E, SC499-14E to make educating for A3 cytoplasmic sterile lines
Property recovered, but most of test strain maintains the sterility of A3 cytoplasmic sterile lines, does not find that its fertility can be made extensive
Multiple Chinese type sorghum strain.
A3 Type Cs MS has two major features：First, anti-abortion is strong, and strong adaptability, stable fertility, second, CHINA SORGHUM is all
Strong with the A3 sterile line talent arch soil power of CHINA SORGHUM transformation for its maintainer, emergence is fast, better resistance, but lacks one due to recovering former
Do not applied in grain sorghum produces directly.Some later researchers, which contemplate, prepares A1, A3 mixed type cenospecies, to solve A1 classes
The problems such as type CMS generally existing annidations are poor, and abortion is serious, and seed production risk is big, but due to increasing kind
The program of son production fails to apply in production always.
In order to solve the problems, such as that A3 cytoplasmic male sterile lines recovery resource is few, Sorghum Breeding man is using the recovery resource of discovery by having
Property selection cross restorer, but restorative measure is carried out in progeny selection to each seed selection strain, give Breeding for restoration lines work to increase
Substantial amounts of work is added.In order to improve A3 restorer efficiencies of selection, we have invented one kind using A3 restoring genes as mark property
Breeding method using A as recurrent parent transformation A3 restorers.
The content of the invention
The technical problem to be solved in the present invention is to overcome program present in existing A3 Breeding for restoration lines is more, workload is big etc.
A kind of problem, there is provided simple, efficient selection of sorghum A3 cytoplasmic male sterility restorers.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
A kind of method using sterile line as recurrent parent seed selection A3 restorers, including selection cross method and backcross breeding method, it is described
Selection cross method comprise the following steps：
The first step：A-grade in the first class 3A × second, without emasculation, pollinated directly to A；
Second step：F1 is selfed；
3rd step：F2 is selfed, and selects that fecundity is good, character fine individual plant；
4th step：F3 selfing purifying, selects that fecundity is good, character fine individual plant；
5th step：F4 characters are basicly stable, due to being the seed selection under A3 cytoplasm backgrounds, so restoring force is strong；
Wherein, second is A3 recovery resources.
Described backcross breeding method comprises the following steps：
The first step：A-grade in the first class 3A × second（A3 recovery resources）, without emasculation, pollinated directly to A；
Second step：F1 is selfed；
3rd step：A-grade in the first class's 3A × F2 fertile plants；
4th step：Bc1 F1 are selfed；
5th step：A-grade in the first class's 3A × Bc1 F2 fertile plants；
6th step：Bc2F1 is selfed；
Same flow is repeatedly returned, and selects the A3 restorers of symbol breeding objective；Wherein, second is A3 recovery resources.
The A3 restorer economical characters having now been found that are all poor, and the economical character for once hybridizing the offspring of acquisition all compares
Difference, backcrossing 2-3 times is generally required, preferable improved effect could be obtained, inventive method is then more shown by recurrent parent of A
Superiority.
The beneficial effect that is reached of the present invention is：
Conventional method sequencing is more, and workload is big, and efficiency is low, and invents the method sequencing provided simply, and workload is small, and the time limit is short,
Efficiency high.
Embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
The selection cross method of embodiment 1.
Conventional method inventive method
First ground beetle × second（A3 recovery resources）A-grade in the first class 3A × second（A3 recovery resources）
Artificial emasculation hybridization does not have to emasculation, is pollinated directly to A
Second step F1 selfing F1 selfings
3rd step F2 is selfed, and selects fine individual plant F2 to be selfed, and selects that fecundity is good, character fine individual plant
4th step F3 test crosses identify restorative F3 selfing purifying, select that fecundity is good, character fine individual plant
Test cross kind progress restorative F4 character of 5th step to F3 generations is basicly stable, due to
Identification, it is under A3 cytoplasm backgrounds to F3 generations according to qualification result
Selected and eliminated, lost excellent single choice and educate, so restoring force is strong.
The possibility 50% of strain
The individual plant that the test of 6th step F4 previous generations fertility recovers
Additive selection, character is basicly stable, extensive
Renaturation also needs further to identify.
The backcross breeding method of embodiment 2.
The A3 restorer economical characters having now been found that are all poor, and it is all poor once to hybridize the economical character of the offspring of acquisition,
Backcrossing 2-3 times is generally required, preferable improved effect could be obtained, the excellent of inventive method is then more shown by recurrent parent of A
More property.
Conventional method inventive method
First ground beetle × second（A3 recovery resources）A-grade in the first class 3A × second（A3 recovery resources）
Artificial emasculation hybridization does not have to emasculation, is pollinated directly to A
Second step F1 selfing F1 selfings
3rd step F2 is selfed, and selects fine individual plant A-grade in the first class 3A × F2 fertile plants
With A3 sterile line test crosses.
4th pacing is handed over（Identify restorative）Bc1 F1 are selfed
Kind of an identification is handed in 5th pacing, according to qualification result A-grade in the first class's 3A × Bc1 F2 fertile plants
Restorative good individual plant again artificial emasculation with
Kind first is returned.
6th step Bc1 F1 selfing Bc2 F1 selfings
↓ ↓
Same flow is repeatedly returned, and selects the A3 restorers of symbol breeding objective.
It can thus be seen that conventional method sequencing is more, workload is big, and efficiency is low, and inventive method sequencing is simple, and workload is small,
The time limit is short, efficiency high.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (2)
1. a kind of method using sterile line as recurrent parent seed selection A3 restorers, including selection cross method and backcross breeding method, its
It is characterised by, described selection cross method comprises the following steps：
The first step：A-grade in the first class 3A × second, without emasculation, pollinated directly to A；
Second step：F1 is selfed；
3rd step：F2 is selfed, and selects that fecundity is good, character fine individual plant；
4th step：F3 selfing purifying, selects that fecundity is good, character fine individual plant；
5th step：F4 characters are basicly stable, due to being the seed selection under A3 cytoplasm backgrounds, so restoring force is strong；
Wherein, second is A3 recovery resources.
A kind of 2. method using sterile line as recurrent parent seed selection A3 restorers according to claim 1, it is characterised in that
Described backcross breeding method comprises the following steps：
The first step：A-grade in the first class 3A × second, without emasculation, pollinated directly to A；
Second step：F1 is selfed；
3rd step：A-grade in the first class's 3A × F2 fertile plants；
4th step：Bc1 F1 are selfed；
5th step：A-grade in the first class's 3A × Bc1 F2 fertile plants；
6th step：Bc2F1 is selfed；
Same flow is repeatedly returned, and selects the A3 restorers of symbol breeding objective；Wherein, second is A3 recovery resources.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711014440.2A CN107517846A (en) | 2017-10-25 | 2017-10-25 | A kind of method using sterile line as recurrent parent seed selection A3 restorers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711014440.2A CN107517846A (en) | 2017-10-25 | 2017-10-25 | A kind of method using sterile line as recurrent parent seed selection A3 restorers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107517846A true CN107517846A (en) | 2017-12-29 |
Family
ID=60685445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711014440.2A Pending CN107517846A (en) | 2017-10-25 | 2017-10-25 | A kind of method using sterile line as recurrent parent seed selection A3 restorers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107517846A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338070A (en) * | 2018-02-26 | 2018-07-31 | 吉林省农业科学院 | A kind of method of selection and breeding sorghum homogeneity restorer |
| CN112753571A (en) * | 2021-03-15 | 2021-05-07 | 四川省农业科学院水稻高粱研究所 | Breeding method of sorghum cytoplasmic male sterile line with high tannin content |
-
2017
- 2017-10-25 CN CN201711014440.2A patent/CN107517846A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 程庆军等: "高粱A3细胞质雄性不育的研究与利用", 《中国农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338070A (en) * | 2018-02-26 | 2018-07-31 | 吉林省农业科学院 | A kind of method of selection and breeding sorghum homogeneity restorer |
| CN112753571A (en) * | 2021-03-15 | 2021-05-07 | 四川省农业科学院水稻高粱研究所 | Breeding method of sorghum cytoplasmic male sterile line with high tannin content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107517846A (en) | A kind of method using sterile line as recurrent parent seed selection A3 restorers | |
| CN104285776A (en) | Breeding method for corn male sterile line | |
| CN105409762A (en) | Cyclic breeding method of cotton varieties | |
| CN1817096A (en) | Japonica rice restoring system breeding method by indica and japonica bridge technology | |
| CN104429918B (en) | A kind of method of Wild cabbage type recessive epistatic interaction core infertility rape temporary maintainer line improvement | |
| CN104115754B (en) | A kind of selection with the radish maintainer of Ogura male sterile cytoplasm | |
| CN103329796B (en) | The universal method of paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing | |
| CN103947541B (en) | Method for breeding rice cytoplasmic male sterile line | |
| CN116472954A (en) | Method for creating clubroot-resistant cabbage pure line | |
| CN1307866C (en) | Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method | |
| CN105830907A (en) | Genic male sterile line of radish and breeding method thereof | |
| CN1127893C (en) | Two-line male sterile method for assortively breeding ecological-type cytoplasm of rape | |
| CN1020390C (en) | Technology for breeding high-yield and quality radish by hybridisation | |
| CN108531635A (en) | The sites corn C type cytoplasmic male sterility restoring gene Rf4 compact linkage molecule label and its application | |
| CN1022079C (en) | Method for breeding cotton through hybridization of three varieties | |
| CN102919116B (en) | Selection breeding method of new homozygous sterile line of brassica napus | |
| CN105961187B (en) | A kind of selection of the polygonal cabbage type rape cytoplasmic male sterility restorer of main inflorescence | |
| CN107299130A (en) | A kind of and molecular labeling chain Stigma Extrusion of Rice QTL and application | |
| Mou et al. | Two-line hybrid rice breeding in and outside China | |
| CN108935068B (en) | Method for improving fertility temperature sensitivity of brassica napus pol CMS sterile line | |
| CN102172212A (en) | Method for breeding new sterile line by use of gene engineering | |
| CN105638447A (en) | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness | |
| CN112753565A (en) | Recurrent selective breeding method of upland cotton based on monogenic dominant genic male sterility | |
| CN107484655B (en) | A kind of selection of anti-clubroot preserved sichuan pickle cytoplasm male sterility hybrid | |
| CN101049082B (en) | Method for carrying out breeding crossbred crop by using male sterile line in type of nucleo-cytoplasmic interaction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171229 |
|
| RJ01 | Rejection of invention patent application after publication |