CN110073971A - A kind of selection of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer - Google Patents
A kind of selection of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer Download PDFInfo
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- CN110073971A CN110073971A CN201910486070.5A CN201910486070A CN110073971A CN 110073971 A CN110073971 A CN 110073971A CN 201910486070 A CN201910486070 A CN 201910486070A CN 110073971 A CN110073971 A CN 110073971A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of selections of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer.Include the following steps: a) to be female parent with the greenery white flower cabbage type rape cytoplasmic male sterility restorer, hybridized, obtain hybrid generation F using the purple green vegetables as male parent1；B) by the hybrid generation F1Selfing obtains F2Plant；C) F is chosen2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is nonrecurrent parent in plant, using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent, carries out 3 backcrossings, obtains backcross progeny BC3F1Plant；D) BC is chosen in selfing3F2Restore fertility in plant 100% and seedling stage leaf is most purple and the florescence spends the highest strain of most white and yield, obtains purple leaf white flower cabbage type rape cytoplasmic male sterility restorer.It can breeding purple leaf white flower Wild cabbage type landscape rape conventional variety using this method.
Description
Technical field
The invention belongs to rapeseed breeding fields, are related to a kind of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer
Selection.
Background technique
Rape is the oil crops planted extensively in world wide, there is oily use, Hua Yong, honey to use, dish is used, is feeding, fertile use
Etc. functions.Cabbage type rape blade is usually that green flower is yellow, and purple leaf white flower rape seedling can cook use, purple leaf oil dish leaf
The anthocyanin that piece is rich in have it is anti-oxidant, antitumor, wait many biology healthcare functions；Purple leaf white flower rape is in seedling stage and colored
Phase is also used as seeing leaf crop for landscape moulding；In addition, the purple leaf white flower character of cabbage type rape can be used as it is a kind of heavy
The indication trait wanted applies to hybrid rape seed can when purple leaf white flower rape is used for hybrid rape seed as indication trait
In seedling stage until flowering stage is easy to the removal of the hybrid strain of non-purple leaf or non-white flower completely, play the difficulty for reducing production of hybrid seeds impurity elimination
Degree improves hybrid rape seed purity, reduces the effect of hybrid rape seed cost.
It is the main approach that rape heterosis utilizes using male sterile line production cenospecies.It selects in the practices of breeding
Educate and be transformed the main path that restorer is breeding and transformation Hybrid.It is choosing that breeding, which has the restorer of purple leaf white flower character,
Educate one of the key technology of purple leaf white flower cross-bred rape kind.
Summary of the invention
The object of the present invention is to provide a kind of breeding sides of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer
Method.
Method provided by the invention is by the purple leaf character transformation of purple green vegetables to greenery white flower cabbage type rape cytoplasm male
In property sterile restoring system, breeding obtains purple leaf white flower cabbage type rape cytoplasmic male sterility restorer.
In the above method, the greenery white flower cabbage type rape cytoplasmic male sterility restorer is white flower 7P71；
The white flower 7P71 is miscellaneous No. 8 complete fertile strains of magnificent oil and through the strain that test cross is 100% recovery and white flower Shanghai
Oil 17 hybridizes, and the white flower for selecting continuous test cross to restore for 100% separates single plant and is selfed to obtain；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
In the above method, the purple leaf character transformation by purple green vegetables to greenery white flower cabbage type rape cytoplasmatic male not
The method educated in restorer includes the following steps:
It a) is mother with the greenery white flower cabbage type rape cytoplasmic male sterility restorer using the purple green vegetables as male parent
This, is hybridized, and hybrid generation F is obtained1；
B) by the hybrid generation F1Selfing obtains F2Plant；
C) F is chosen2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-circulation in plant
Parent carries out 3 backcrossings, obtains using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
Backcross progeny BC3F1Plant；
3 backcrossings are that selection seedling stage leaf is most purple from last backcross progeny and florescence flower is most white and 100% is extensive
The individual plant selfing of multiple fertility, then selection seedling stage leaf is most purple from self progeny and florescence flower is most white and 100% restores fertility single plant
As the nonrecurrent parent being returned next time；
D) the backcross progeny BC is chosen3F1Seedling stage leaf is most purple in plant and florescence flower is most white and 100% restores fertility single plant
Selfing, obtains BC3F2For plant, BC is chosen3F2For in plant 100% restore fertility and seedling stage leaf is most purple and florescence flower is most white and
The highest strain of yield obtains purple leaf white flower cabbage type rape cytoplasmic male sterility restorer.
In the above method, 3 backcrossings include the following:
C-1, the F is chosen2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-wheel in plant
Parent is returned, using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent, is returned 1 time, is returned
Hand over offspring BC1F1Plant；Again from the backcross progeny BC1F1Selection 100% restores fertility in plant and seedling stage leaf is most purple and the florescence
The most white individual plant selfing of flower, obtains BC1F2Plant；
C-2, the BC is chosen1F2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-in plant
Recurrent parent is returned 2 times, obtains using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
Backcross progeny BC2F1Plant；Again from the backcross progeny BC2F1Selection 100% restores fertility in plant and seedling stage leaf is most purple and colored
The most white individual plant selfing of phase flower, obtains BC2F2Plant；
C-3, the BC is chosen2F2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-in plant
Recurrent parent is returned 3 times, obtains using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
Backcross progeny BC3F1Plant.
In the above method, in c-1, the selection F2In plant 100% restore fertility and seedling stage leaf is most purple and florescence flower most
White single plant is that the single plant quantity of nonrecurrent parent is 20；
Or c-1) into c-3), choose that seedling stage leaf is most purple and florescence flower is most white from backcross progeny plant every time and 100%
The single plant of recovery fertility is 20 as the single plant quantity of nonrecurrent parent；
Or c-1) into c-3), chosen from backcross progeny every time for selfing seedling stage leaf is most purple and florescence flower is most white and
The 100% single plant quantity for restoring fertility is 2.
In the above method, the selection backcross progeny BC3F1Seedling stage leaf is most purple in plant and florescence flower is most white and 100% is extensive
The quantity of multiple fertility individual plant selfing is 10.
In the above method, hybridize in the method, be selfed, backcrossing and the place being selfed staggeredly carry out.
Greenery white flower cabbage type rape cytoplasmic male sterility restorer white flower 7P71 is thin in purple leaf white flower cabbage type rape
Application in the breeding of cytoplasmic male sterilty restorer is also the scope of protection of the invention；
The white flower 7P71 is miscellaneous No. 8 complete fertile strains of magnificent oil and through the strain that test cross is 100% recovery and white flower Shanghai
Oil 17 hybridizes, and the white flower for selecting continuous test cross to restore for 100% separates single plant and is selfed to obtain；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
Above-mentioned 100% restores fertility as the strain and cabbage type rape cytoplasmic male sterile line (such as miscellaneous No. 8 of China's oil) survey
It hands over, sterile line 100% is made to restore fertility.
In the above method, the white flower cabbage type rape cytoplasmic male sterility restorer is white flower 7P71, for China's oil miscellaneous 8
The complete fertile strain of number (state examine oil 2004007) separation is 100% strain restored and 17 (Shanghai oil 17 of white flower Shanghai oil through test cross
(breeding of crop institute, Shanghai academy of agricultural sciences, state examine oil 2011006) white flower mutant strain, deposit number CGMCCNo.17384, the micro- life of China
Object culture presevation administration committee common micro-organisms center, abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, Institute of Microorganism, Academia Sinica, postcode 100101) hybridization, and test cross is that the 100% separation single plant restored is selected through system
It educates.
White flower Shanghai oil 17, deposit number are CGMCC No.17384, and China Committee for Culture Collection of Microorganisms is common
Microorganism center, abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101, preservation day are on 2 28th, 2019, and classification naming is cabbage type rape Brassicanapus.
Tests prove that the purple leaf character of purple green vegetables can be passed through hybridization, backcrossing, test cross technology transformation to white flower
In cabbage type rape cytoplasmic male sterility restorer, the excellent cytoplasmic male sterility with purple leaf white flower character of breeding is extensive
Multiple system.
Detailed description of the invention
Fig. 1 is purple leaf white flower cabbage type rape cytoplasmic male sterility restorer purple 7P71 breeding flow chart.
Fig. 2 is different times purple leaf white flower cabbage type rape cytoplasmic male sterility restorer；
A, seedling stage purple leaf white flower cabbage type rape cytoplasmic male sterility restorer；
B, squaring period purple leaf white flower cabbage type rape cytoplasmic male sterility restorer；
C, peduncle-growing period for rapeseed purple leaf white flower cabbage type rape cytoplasmic male sterility restorer.
Fig. 3 is that purple leaf white flower Brassica napus hybrid combines breeding flow chart.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1, breeding purple leaf white flower cabbage type rape cytoplasmic male sterility restorer purple 7P71
One, the breeding of white flower cabbage type rape cytoplasmic male sterility restorer white flower 7P71
The complete fertile strain of miscellaneous No. 8 of magnificent oil (state examines oil 2004007) separation is maternal through the strain that test cross is 100% recovery
Hybridize with male parent white flower Shanghai oil 17, hybridizes F1Bagging separates, and choosing range estimation is for white flower and oily No. 8 miscellaneous (state examines oil 2004007) with China
Separating sterile plant test cross is the 100% separation single plant restored, is selfed and synchronizes through continuous multi-generation and mostly restores white for test cross 100%
Flower single plant systematic breeding forms, and names as white flower 7P71, is white flower cabbage type rape cytoplasmic male sterility restorer.
White flower Shanghai oil 17, deposit number are CGMCC No.17384, and China Committee for Culture Collection of Microorganisms is common
Microorganism center, abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101, preservation day are on 2 28th, 2019, and classification naming is cabbage type rape Brassicanapus.
Two, hybridize transformation
In March, 2010 carries out hybridization transformation according to Fig. 1:
1, hybridize
In March, 2010 is in Hefei with 2 plants of purple green vegetables (Fang Zhenzi green vegetables, production number: 7095, Hebei Qing County rich kind of industry of grain grain)
Be female parent with 5 plants of white flower cabbage type rape cytoplasmic male sterility restorer white flower 7P71 for male parent, hybridization, obtain 10 parts it is miscellaneous
Hand over offspring F1Seed；
2, it is selfed
In May, 2010, by 10 parts of F of harvest1Hybrid seed takes Qinghai to, and in July, 2010 is from 10 parts of F1In respectively select
100% recovery fertility and 2 plants of baggings selfings of single plant that seedling stage leaf is most purple and florescence flower is most white, obtain 20 plants of F2Selfed seed；
3, repeatedly backcrossing
1) it is returned for the first time
In September, 2010, in 20 plants of F of plantation Qinghai, Hefei harvest2Selfed seed, every plant of F2It is selfed strain and plants 2 rows, totally 20
A F2Strain.In March, 2011 is from 20 F2100% recovery fertility of selection (refers to and cabbage type rape cytoplasmic male sterility in strain
System (such as the isolated sterile plant of China's oil miscellaneous No. 8 selfings) test cross, makes the recovery of sterile line 100% fertility) and seedling stage leaf is most purple and the florescence
Most white 20 plants are spent, with 20 plants of F2As male parent (nonrecurrent parent), 10 plants of white flower 7P71 are maternal (recurrent parent), are returned,
Obtain 10 parts of 1 generation group BC of backcrossing1F1Seed；
In May, 2011, by 10 parts of 1 generation group BC of backcrossing of harvest1F1Seed takes Qinghai plantation to, obtains 10 parts of BC1F1It plants
Strain；In July, 2011 is from 10 parts of BC1F1The single plant 2 that selection 100% restores fertility in plant and seedling stage leaf is most purple and florescence flower is most white
Strain bagging selfing, obtains 20 plants of selfing strain seeds and is denoted as BC1F2。
In September, 2011, in 20 plants of BC of plantation Qinghai, Hefei harvest1F2Seed obtains BC1F2Plant, every plant of 2 rows of plantation,
Totally 20 BC1F2Strain.
2) the 2nd backcrossing
In March, 2012 is from 20 BC1F2Selection 100% restores fertility in strain and seedling stage leaf is most purple and florescence flower is most white
20 plants of single plant；With this 20 plants of BC1F2As male parent (nonrecurrent parent), 10 plants of white flower 7P71 are maternal (recurrent parent), are returned,
Obtain 10 parts of 2 generation group BC of backcrossing2F1Seed；
In May, 2012, by 20 parts of BC of harvest2F1Seed takes Qinghai plantation to, obtains BC2F1Plant, in July, 2012 from
20 parts of BC2F12 plants of bagging selfings of the single plant that selection 100% restores fertility in plant and seedling stage leaf is most purple and florescence flower is most white, obtain
20 plants of selfing strain seeds, are denoted as BC2F2；
In September, 2012, in 20 plants of BC of plantation Qinghai, Hefei harvest2F2Seed obtains BC2F2Plant, every plant of 2 rows of plantation,
Totally 20 BC2F2Strain.
3) the 3rd backcrossing
In March, 2013 is from 20 BC2F2Selection 100% restores fertility in strain and seedling stage leaf is most purple and florescence flower is most white
20 plants of single plant；With this 20 plants of BC2F2As male parent (nonrecurrent parent), 20 plants of white flower 7P71 are maternal (recurrent parent), are returned,
Obtain 20 parts of 3 generation group BC of backcrossing3F1Seed；
In May, 2013, by 20 parts of BC of harvest3F1Seed takes Qinghai plantation to, obtains BC3F1Plant, in July, 2013 from
10 parts of BC3F110 plants of bagging selfings of the single plant that selection 100% restores fertility in plant and seedling stage leaf is most purple and florescence flower is most white, are obtained
It obtains 10 plants of selfing strains and is denoted as BC3F2Seed；
10 parts of BC of Qinghai harvest are planted in September, 2013, Hefei3F2Seed obtains BC3F2Plant, every part is planted 100 plants,
Totally 10 strains.
In March, 2014 is in 10 BC3F2Breeding is isolated with the nylon wire accounts of 100 mesh respectively before just spending in strain, at the florescence,
Each strain is each to take 5 plants and cabbage type rape cytoplasmic male sterile line test cross at random；Investigate the seedling stage purple leaf flower of 10 strains
Phase pattern character；10 strain test cross results are identified in Qinghai in July, 2014；After rape is mature, plant division system harvest, which is surveyed, to be produced.Screening is surveyed
5 plant of 100% recovery is handed over, selects purple leaf white flower cabbage type rape in the most highest strain conduct of the most white and yield of pale reddish brown phase flower of seedling stage leaf
Cytoplasmic male sterility restorer is named as purple 7P71.
From the figure of purple leaf white flower Brassica napus cytoplasmic male sterility restorer purple 7P71 different times as shown in Fig. 2, A,
Seedling stage purple leaf white flower cabbage type rape cytoplasmic male sterility restorer；B, squaring period purple leaf white flower cabbage type rape cytoplasm male
Property sterile restoring system；C, peduncle-growing period for rapeseed purple leaf white flower cabbage type rape cytoplasmic male sterility restorer；As can be seen that seedling stage range estimation
Purple leaf character is obvious and consistent.
Fig. 3 is that purple leaf white flower Brassica napus hybrid combines breeding flow chart.
Claims (8)
1. a kind of selection of purple leaf white flower cabbage type rape cytoplasmic male sterility restorer, is by the purple leaf of purple green vegetables
Into greenery white flower cabbage type rape cytoplasmic male sterility restorer, it is thin that breeding obtains purple leaf white flower cabbage type rape for shape transformation
Cytoplasmic male sterilty restorer.
2. according to the method described in claim 1, it is characterized by: the greenery white flower cabbage type rape cytoplasmic male sterility
Restorer is white flower 7P71；
The white flower 7P71 is miscellaneous No. 8 complete fertile strains of magnificent oil and through the strain that test cross is 100% recovery and white flower Shanghai oil 17
Hybridization, the white flower for selecting continuous test cross to restore for 100% separate single plant and are selfed to obtain；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
3. method according to claim 1 or 2, it is characterised in that:
The purple leaf character transformation by purple green vegetables is to the side in greenery white flower cabbage type rape cytoplasmic male sterility restorer
Method includes the following steps:
It a) is female parent with the greenery white flower cabbage type rape cytoplasmic male sterility restorer using the purple green vegetables as male parent,
Hybridized, obtains hybrid generation F1；
B) by the hybrid generation F1Selfing obtains F2Plant；
C) F is chosen2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is nonrecurrent parent in plant,
Using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent, 3 backcrossings are carried out, after obtaining backcrossing
For BC3F1Plant；
3 backcrossings are that selection seedling stage leaf is most purple from last backcross progeny and florescence flower is most white and 100% restores to educate
Property individual plant selfing, then choose from self progeny that seedling stage leaf is most purple and florescence flower is most white and 100% restores the conduct of fertility single plant
The nonrecurrent parent being returned next time；
D) the backcross progeny BC is chosen3F1Seedling stage leaf is most purple in plant and florescence flower is most white and 100% restores fertility single plant certainly
It hands over, obtains BC3F2For plant, BC is chosen3F2Restore fertility in plant 100% and seedling stage leaf is most purple and florescence flower is most white and produces
Highest strain is measured, purple leaf white flower cabbage type rape cytoplasmic male sterility restorer is obtained.
4. according to the method described in claim 3, it is characterized by:
3 backcrossings include the following:
C-1, the F is chosen2100% recovery fertility and the single plant that seedling stage leaf is most purple and florescence flower is most white are non-circulation parent in plant
This is returned 1 time, after obtaining backcrossing using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
For BC1F1Plant；Again from the backcross progeny BC1F1Selection 100% restores fertility in plant and seedling stage leaf is most purple and the florescence is spent most
White individual plant selfing, obtains BC1F2Plant；
C-2, the BC is chosen1F2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-circulation in plant
Parent is returned 2 times, is returned using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
Offspring BC2F1Plant；Again from the backcross progeny BC2F1Selection 100% restores fertility in plant and seedling stage leaf is most purple and the florescence is spent
Most white individual plant selfing, obtains BC2F2Plant；
C-3, the BC is chosen2F2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-circulation in plant
Parent is returned 3 times, is returned using the greenery white flower cabbage type rape cytoplasmic male sterility restorer as recurrent parent
Offspring BC3F1Plant.
5. according to the method described in claim 4, it is characterized by:
In c-1, the selection F2100% restores fertility and seedling stage leaf is most purple and florescence flower is most white single plant is non-circulation in plant
The single plant quantity of parent is 20；
Or c-1) into c-3), selection seedling stage leaf is most purple from backcross progeny plant every time and florescence flower is most white and 100% restores
The single plant of fertility is 20 as the single plant quantity of nonrecurrent parent；
Or c-1) into c-3), chosen from backcross progeny every time for selfing seedling stage leaf is most purple and florescence flower is most white and
The 100% single plant quantity for restoring fertility is 2.
6. according to the method any in claim 3-5, it is characterised in that:
The selection backcross progeny BC3F1Seedling stage leaf is most purple in plant and florescence flower is most white and 100% restores fertility individual plant selfing
Quantity is 10.
7. according to the method any in claim 3-6, it is characterised in that: hybridize in the method, be selfed, backcrossing and from
The place of friendship staggeredly carries out.
8. greenery white flower cabbage type rape cytoplasmic male sterility restorer white flower 7P71 is in purple leaf white flower cabbage type rape cell
Application in the breeding of matter male sterile restoring line；
The white flower 7P71 is miscellaneous No. 8 complete fertile strains of magnificent oil and through the strain that test cross is 100% recovery and white flower Shanghai oil 17
Hybridization, the white flower for selecting continuous test cross to restore for 100% separate single plant and are selfed to obtain；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
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| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
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| CN105900831A (en) * | 2016-05-12 | 2016-08-31 | 安徽农业大学 | Breeding method of main-inflorescence multi-silique brassica napus type conventional rape variety |
| CN105961187A (en) * | 2016-05-12 | 2016-09-28 | 安徽农业大学 | Breeding method of main inflorescence polyhedron brassica napus cytoplasm male sterility restorer line |
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| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
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