CN110100722A - A kind of selection of purple leaf white flower Wild cabbage type landscape rape conventional variety - Google Patents
A kind of selection of purple leaf white flower Wild cabbage type landscape rape conventional variety Download PDFInfo
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- CN110100722A CN110100722A CN201910486526.8A CN201910486526A CN110100722A CN 110100722 A CN110100722 A CN 110100722A CN 201910486526 A CN201910486526 A CN 201910486526A CN 110100722 A CN110100722 A CN 110100722A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of selections of purple leaf white flower Wild cabbage type landscape rape conventional variety.Include the following steps: a) to be female parent with the greenery white flower cabbage type rape strain, hybridized, obtain hybrid generation F using the purple green vegetables as male parent1；B) by the hybrid generation F1Selfing obtains F2Plant；C) F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, using the greenery white flower cabbage type rape strain as recurrent parent, carries out 3 backcrossings, obtains backcross progeny BC3F1Plant；D) by the backcross progeny BC3F1Plant selfing obtains BC3F2For plant, BC is chosen3F2And florescence most purple for seedling stage leaf in the plant flower highest strain of most white and yield, obtains purple leaf white flower Wild cabbage type normal rapeseed kind.It can breeding purple leaf white flower Wild cabbage type landscape rape conventional variety using this method.
Description
Technical field
The invention belongs to rapeseed breeding fields, are related to a kind of breeding side of purple leaf white flower Wild cabbage type landscape rape conventional variety
Method.
Background technique
Rape is the oil crops planted extensively in world wide.China is Rape-seed production big country, long-term cultivated area about 1
Hundred million mu, area and yield are all at the forefront in the world, but in recent years, and rapeseed area and total output but glide, and plantation rape compares
Low benefit is main reason, and Yao Tigao rapeseed cultivation comparative effectiveness, the multi-functional utilization for developing rape is one of effective measures.
There is rape oily uses, Hua Yong, honey to use, dish is used, is feeding, it is fertile with etc. six functions, wherein rape flower with mainly by
Rape is cultivated as ornamental crops, and for watching, but rape is as landscape crop, especially for there is the oil of landscape design
Cauliflower sea, can only enjoy pattern and Hua Hai at the florescence, cannot attract tourists in seedling stage.Therefore, breeding one kind can be in seedling stage
See leaf, the florescence sees colored landscape rape and is of great significance for the exploitation multi-functional utilization of rape.
Summary of the invention
The object of the present invention is to provide a kind of selections of purple leaf white flower Wild cabbage type landscape rape conventional variety.
Method provided by the invention, be by the purple leaf character transformation of purple green vegetables into greenery white flower cabbage type rape strain,
Breeding obtains purple leaf white flower Wild cabbage type normal rapeseed kind.
In the above method, the greenery white flower cabbage type rape strain is ZH17；
The ZH17 be in double 11 and 17 hybridization of white flower Shanghai oil after selfed breeding form；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
In the above method, the purple leaf character transformation by purple green vegetables to the method in greenery white flower cabbage type rape strain
Include the following steps:
A) using the purple green vegetables as male parent, it is female parent with the greenery white flower cabbage type rape strain, is hybridized, obtained
Hybrid generation F1；
B) by the hybrid generation F1Selfing obtains F2Plant；
C) F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with the greenery
White flower cabbage type rape strain is recurrent parent, carries out 3 backcrossings, obtains backcross progeny BC3F1Plant；
3 backcrossings are that selection seedling stage leaf is most purple from last backcross progeny and the florescence spends most white single plant certainly
It hands over, then selection seedling stage leaf is most purple from self progeny and the florescence most white single plant of flower is as the nonrecurrent parent being returned next time；
D) the backcross progeny BC is chosen3F1The individual plant selfing that seedling stage leaf is most purple in plant and florescence flower is most white, obtains
BC3F2For plant, BC is chosen3F2And florescence most purple for seedling stage leaf in the plant flower highest strain of most white and yield, obtains purple Ye Bai
Flower Wild cabbage type normal rapeseed kind.
In the above method, 3 backcrossings include the following:
C-1, the F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with described green
Leaf white flower cabbage type rape strain is recurrent parent, is returned 1 time, obtains backcross progeny BC1F1Plant；Again from the backcross progeny
BC1F1The individual plant selfing that seedling stage leaf is most purple and florescence flower is most white is selected in plant, obtains BC1F2Plant；
C-2, the BC is chosen1F2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with described
Greenery white flower cabbage type rape strain is recurrent parent, is returned 2 times, obtains backcross progeny BC2F1Plant；Again after the backcrossing
For BC2F1The individual plant selfing that plant selects seedling stage leaf most purple and florescence flower is most white, obtains BC2F2Plant；
C-3, the BC is chosen2F2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with described
Greenery white flower cabbage type rape strain is recurrent parent, is returned 3 times, obtains backcross progeny BC3F1Plant.
In the above method, c-1) in, choose the F2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is non-wheel
The single plant quantity for returning parent is 10；
Or, c-2) into c-3), every time from backcross progeny plant choose as nonrecurrent parent seedling stage leaf it is most purple and
The most white single plant quantity of florescence flower is 10；
Or, c-1) into c-3), seedling stage leaf of the selection for selfing is most purple from backcross progeny every time and florescence flower is most white
Single plant quantity be 2.
In the above method, d) in, choose the backcross progeny BC3F1The list that seedling stage leaf is most purple in plant and florescence flower is most white
The quantity of strain is 10.
In the above method, hybridize in the method, be selfed, backcrossing and the place being selfed staggeredly carry out.
Greenery white flower cabbage type rape strain ZH17 answering in purple leaf white flower Wild cabbage type landscape rape conventional variety breeding
With being also the scope of protection of the invention；
The ZH17 be in double 11 and 17 hybridization of white flower Shanghai oil after selfed breeding form；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
In the above method, the white flower cabbage type rape strain is Wild cabbage type normal rapeseed strain white flower ZH17, white flower
(Inst. of Oil Crops, Chinese Academy of Agriculture's breeding, 20008030) it is female parent and male parent Shanghai oil that state examines oil to ZH17 in double 11
17 (breeding of crop institute, Shanghai academy of agricultural sciences, after state examines oily 2011006) white flower mutant strain " white flower Shanghai oil 17 " hybridization, continuous 5 generation set
Bag selfing, and formed as objective trait through systematic breeding using estimating white flower.
White flower Shanghai oil 17, deposit number are CGMCC No.17384, and China Committee for Culture Collection of Microorganisms is common
Microorganism center, abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101, preservation day are on 2 28th, 2019, and classification naming is cabbage type rape Brassica napus.
Tests prove that the purple leaf character of purple green vegetables can be passed through hybridization, baclccrossing techniques transformation to white flower wild cabbage
In type napus lines, the excellent conventional oil with purple leaf white flower character of breeding (seedling stage leaf is purple, full-bloom stage flower is white)
Vegetable kind sees leaf as seedling stage, the florescence sees flower, seed oil expression multifunctional landscape rape；It is purple, full-bloom stage with the seedling stage leaf
When flower is provided commonly for the rape flower sea of landscape design with the ordinary cole of greenery chrysanthemum for the landscape rape variety of white,
It may insure to attract tourists in seedling stage but also at the florescence, extend rape as landscape arable farming timeliness, increase income.
Detailed description of the invention
Fig. 1 is the purple oily No. 1 breeding flow chart of purple leaf white flower Wild cabbage type landscape rape conventional variety.
Fig. 2 is purple green vegetables.
Fig. 3 is that purple leaf white flower Wild cabbage type landscape rape and greenery cabbage type rape blade compare.
Fig. 4 is purple leaf white flower Wild cabbage type landscape rape and greenery cabbage type rape contrast of seedling stage (left, white flower ZH17；The right side, it is purple
Oil 1).
Fig. 5 is purple leaf white flower Wild cabbage type landscape rape and greenery cabbage type rape squaring period comparison (left, purple oil 1；The right side,
White flower ZH17).
Fig. 6 is purple leaf white flower Wild cabbage type landscape rape peduncle-growing period for rapeseed.
Fig. 7 is purple leaf white flower Wild cabbage type landscape brassica napus inflorescence.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1, breeding purple leaf white flower Wild cabbage type landscape rape conventional variety
One, the breeding of Wild cabbage type normal rapeseed strain white flower ZH17
White flower ZH17: in double 11 (Inst. of Oil Crops, Chinese Academy of Agriculture's breeding, state examine oil and 20008030) are
It is maternal that (breeding of crop institute, Shanghai academy of agricultural sciences, state examines oil, and 2011006) white flower mutant strain " white flower Shanghai oil 17 " is miscellaneous with male parent Shanghai oil 17
After friendship, continuous 5 generation bagging selfing, and formed as objective trait through systematic breeding using estimating white flower.
White flower Shanghai oil 17, deposit number are CGMCC No.17384, and China Committee for Culture Collection of Microorganisms is common
Microorganism center, abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101, preservation day are on 2 28th, 2019, and classification naming is cabbage type rape Brassica napus.
Two, hybridize transformation
In March, 2010 carries out hybridization transformation according to Fig. 1:
1, hybridize
In March, 2010 Hefei with 2 plants of purple green vegetables (Fang Zhenzi green vegetables, production number: 7095, Hebei Qing County grain rich kind of industry of grain；
Fig. 2) it is male parent, is female parent with 5 plants of white flower cabbage type rape strain white flower ZH17, hybridization obtains 10 parts of filial generation F1Seed；
2, it is selfed
In May, 2010, by 10 parts of F of harvest1Hybrid seed takes Qinghai to, and in July, 2010 is from 10 parts of F1In respectively select seedling stage
2 plants of bagging selfings of the single plant that leaf is most purple and florescence flower is most white, obtain 20 plants of F2Selfed seed；
3, repeatedly backcrossing
1) it is returned for the first time
In September, 2010, in 20 plants of F of plantation Qinghai, Hefei harvest2Selfed seed, every plant of F2It is selfed strain and plants 2 rows, totally 20
A F2Strain.In March, 2011 is from 20 F2Selection seedling stage leaf is most purple in strain and the florescence spends most white 10 plants of single plant；With 10 plants of F2
As male parent (nonrecurrent parent), 10 plants of white flower ZH17 are maternal (recurrent parent), and backcrossing obtains 10 parts of 1 generation groups of backcrossing
BC1F1Seed；
In May, 2011, by 10 parts of 1 generation group BC of backcrossing of harvest1F1Seed takes Qinghai plantation to, obtains 10 parts of BC1F1It plants
Strain；In July, 2011 is from 10 parts of BC1F12 plants of bagging selfings of single plant that seedling stage leaf is most purple and florescence flower is most white are selected in plant, are obtained
20 plants of selfing strain seeds are denoted as BC1F2。
In September, 2011, in 20 plants of BC of plantation Qinghai, Hefei harvest1F2Seed obtains BC1F2Plant, every plant of 2 rows of plantation,
Totally 20 BC1F2Strain.
2) the 2nd backcrossing
In March, 2012 is from 20 BC1F2Selection seedling stage leaf is most purple in strain and the florescence spends most white 10 plants of single plant；With this 10
Strain BC1F2As male parent (nonrecurrent parent), 10 plants of white flower ZH17 are maternal (recurrent parent), and backcrossing obtains 10 parts of 2 generations of backcrossing
Group BC2F1Seed；
In May, 2012, by 10 parts of BC of harvest2F1Seed takes Qinghai plantation to, obtains BC2F1Plant, in July, 2012 from
10 parts of BC2F12 plants of bagging selfings of single plant that seedling stage leaf is most purple and florescence flower is most white are selected in plant, obtain 20 plants of selfing strain seeds,
It is denoted as BC2F2；
In September, 2012, in 20 plants of BC of plantation Qinghai, Hefei harvest2F2Seed obtains BC2F2Plant, every plant of 2 rows of plantation,
Totally 20 BC2F2Strain.
3) the 3rd backcrossing
In March, 2013 is from 20 BC2F2Selection seedling stage leaf is most purple in strain and the florescence spends most white 10 plants of single plant；With this 10
Strain BC2F2As male parent (nonrecurrent parent), 10 plants of white flower ZH17 are maternal (recurrent parent), and backcrossing obtains 10 parts of 3 generations of backcrossing
Group BC3F1Seed；
In May, 2013, by 10 parts of BC of harvest3F1Seed takes Qinghai plantation to, obtains BC3F1Plant, in July, 2013 from
10 parts of BC3F110 plants of bagging selfings of single plant that seedling stage leaf is most purple and florescence flower is most white are selected in plant, are obtained 10 plants of selfing strains and are denoted as
BC3F2Seed；
10 parts of BC of Qinghai harvest are planted in September, 2013, Hefei3F2Seed obtains BC3F2Plant, every part is planted 100 plants,
Totally 10 strains.
In March, 2014 is in 10 BC3F2Breeding, maturity period tune is isolated with the nylon wire account of 100 mesh respectively before just spending in strain
The purple leaf white flower characters for looking into the strain of 10 set accounts isolation breedings, after rape is mature, harvest, which is surveyed, to be produced, screening seedling stage purple leaf it is most purple and
Florescence white flower is most white and the highest strain of yield is as target purple leaf white flower Wild cabbage type landscape rape conventional variety, names as purple oil
No. 1.
Fig. 3 is that purple leaf white flower Wild cabbage type landscape rape and greenery cabbage type rape blade compare.
By common greenery cabbage type rape (white flower ZH17) and purple leaf white flower wild cabbage landscape rape (purple oil 1) seedling stage pair
Than as a result such as Fig. 4.Common greenery cabbage type rape (white flower ZH17) and purple leaf white flower wild cabbage landscape rape (purple oil 1) is existing
Flower bud phase comparison, as a result such as Fig. 5.As can be seen that purple leaf white flower rape (right, purple oil 1) seedling stage, to estimate purple leaf character obvious and one
It causes.Fig. 6 is purple leaf white flower Wild cabbage type landscape rape peduncle-growing period for rapeseed.Fig. 7 is purple leaf white flower Wild cabbage type landscape brassica napus inflorescence.
Claims (8)
1. a kind of selection of purple leaf white flower Wild cabbage type landscape rape conventional variety, is to arrive the purple leaf character transformation of purple green vegetables
In greenery white flower cabbage type rape strain, breeding obtains purple leaf white flower Wild cabbage type normal rapeseed kind.
2. according to the method described in claim 1, it is characterized by: the greenery white flower cabbage type rape strain is ZH17；
The ZH17 be in double 11 and 17 hybridization of white flower Shanghai oil after selfed breeding form；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
3. method according to claim 1 or 2, it is characterised in that:
The purple leaf character transformation by purple green vegetables includes the following steps: to the method in greenery white flower cabbage type rape strain
A) using the purple green vegetables as male parent, it is female parent with the greenery white flower cabbage type rape strain, is hybridized, hybridized
Filial generation F1；
B) by the hybrid generation F1Selfing obtains F2Plant；
C) F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with the greenery white flower
Cabbage type rape strain is recurrent parent, carries out 3 backcrossings, obtains backcross progeny BC3F1Plant；
3 backcrossings are the individual plant selfing that selection seedling stage leaf is most purple from last backcross progeny and florescence flower is most white, then
Selection seedling stage leaf is most purple from self progeny and the florescence most white single plant of flower is as the nonrecurrent parent being returned next time；
D) the backcross progeny BC is chosen3F1The individual plant selfing that seedling stage leaf is most purple in plant and florescence flower is most white, obtains BC3F2Dai Zhi
BC is chosen in strain3F2And florescence most purple for seedling stage leaf in the plant flower highest strain of most white and yield, obtains purple leaf white flower Wild cabbage type
Normal rapeseed kind.
4. according to the method described in claim 3, it is characterized by:
3 backcrossings include the following:
C-1, the F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, white with the greenery
Flower cabbage type rape strain is recurrent parent, is returned 1 time, obtains backcross progeny BC1F1Plant；Again from the backcross progeny BC1F1
The individual plant selfing that seedling stage leaf is most purple and florescence flower is most white is selected in plant, obtains BC1F2Plant；
C-2, the BC is chosen1F2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with the greenery
White flower cabbage type rape strain is recurrent parent, is returned 2 times, obtains backcross progeny BC2F1Plant；Again from the backcross progeny
BC2F1The individual plant selfing that plant selects seedling stage leaf most purple and florescence flower is most white, obtains BC2F2Plant；
C-3, the BC is chosen2F2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is nonrecurrent parent, with the greenery
White flower cabbage type rape strain is recurrent parent, is returned 3 times, obtains backcross progeny BC3F1Plant.
5. according to the method described in claim 4, it is characterized by:
C-1 in), the F is chosen2The single plant that seedling stage leaf is most purple in plant and florescence flower is most white is the single plant quantity of nonrecurrent parent
It is 10；
Or, c-2) into c-3), chosen from backcross progeny plant every time as nonrecurrent parent seedling stage leaf is most purple and the florescence
The most white single plant quantity of flower is 10；
Or, c-1) into c-3), the list that the seedling stage leaf for being used for being selfed is most purple and florescence flower is most white is chosen from backcross progeny every time
Strain number amount is 2.
6. according to the method any in claim 3-5, it is characterised in that:
D) in, the backcross progeny BC is chosen3F1The quantity for the single plant that seedling stage leaf is most purple in plant and florescence flower is most white is 10.
7. according to the method any in claim 3-6, it is characterised in that: hybridize in the method, be selfed, backcrossing and from
The place of friendship staggeredly carries out.
8. application of the greenery white flower cabbage type rape strain ZH17 in purple leaf white flower Wild cabbage type landscape rape conventional variety breeding；
The ZH17 be in double 11 and 17 hybridization of white flower Shanghai oil after selfed breeding form；
The deposit number of white flower Shanghai oil 17 is CGMCC No.17384.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
| CN112438199A (en) * | 2020-12-18 | 2021-03-05 | 湖南省作物研究所 | Breeding method of rape variety suitable for balcony cultivation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN105900831A (en) * | 2016-05-12 | 2016-08-31 | 安徽农业大学 | Breeding method of main-inflorescence multi-silique brassica napus type conventional rape variety |
-
2019
- 2019-06-05 CN CN201910486526.8A patent/CN110100722B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN105900831A (en) * | 2016-05-12 | 2016-08-31 | 安徽农业大学 | Breeding method of main-inflorescence multi-silique brassica napus type conventional rape variety |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
| CN112438199A (en) * | 2020-12-18 | 2021-03-05 | 湖南省作物研究所 | Breeding method of rape variety suitable for balcony cultivation |
| CN112438199B (en) * | 2020-12-18 | 2021-11-02 | 湖南省作物研究所 | Breeding method of rape variety suitable for balcony cultivation |
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