WO2010101274A1 - Seedless capsicum annuum l. and method for producing same - Google Patents

Seedless capsicum annuum l. and method for producing same Download PDF

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Publication number
WO2010101274A1
WO2010101274A1 PCT/JP2010/053731 JP2010053731W WO2010101274A1 WO 2010101274 A1 WO2010101274 A1 WO 2010101274A1 JP 2010053731 W JP2010053731 W JP 2010053731W WO 2010101274 A1 WO2010101274 A1 WO 2010101274A1
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capsicum annuum
cms
nat
fruit
line
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PCT/JP2010/053731
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French (fr)
Japanese (ja)
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伊藤智司
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横浜植木株式会社
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Priority to JP2011502833A priority Critical patent/JP5272071B2/en
Publication of WO2010101274A1 publication Critical patent/WO2010101274A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A01H1/023—Male sterility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/08—Fruits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/822—Capsicum sp. [pepper]

Definitions

  • the present invention relates to a nuclear-free capsicum annuum (L.) and a method for producing the same.
  • Non-Patent Document 1 a nuclear-free tomato has been developed (Patent Document 1).
  • the tomato is produced by crossing both a male-sterile tomato strain containing a partheno-result gene as a parent and a tomato strain containing a part-effect gene as a parent.
  • non-nucleated tomatoes can be easily produced using a chemical such as Tomato Tone (registered trademark, Ishihara Sangyo Co., Ltd.).
  • Tomato Tone registered trademark, Ishihara Sangyo Co., Ltd.
  • an anuclear eggplant variety utilizing cytoplasmic male sterility is being bred (Non-Patent Document 1).
  • An object of the present invention is to provide a nuclear-free capsicum annuum and a method for producing the same.
  • the present inventor found a cytoplasmic male sterile line in capsicum annuum during breeding, and various varieties of capsicum annuum in the line of capsicum annuum. Crossed the Annuum and succeeded in obtaining a stable and non-nuclear Capsicum Annuum. That is, the present invention includes the following inventions.
  • Capsicum annuum L. “CMS system KY111” accession number FERM BP-11223 which is cytoplasmic male sterile and does not have a fertility recovery gene.
  • Capsicum annuum L. “CMS NAT” (accession number FERM BP-11225), which is cytoplasmic male sterile and does not have a fertility recovery gene.
  • CMS KY111 accession number FERM BP-112263
  • CMS NAT accession number FERM BP-11225
  • Capsicum annuum (Capsicum annuum L.) includes cultivated species such as so-called bell peppers, paprika, chili pepper, and shishitou.
  • “Kapushikum annuum” includes plants, seeds, fruits, and those grown by vegetative propagation or seed propagation.
  • CMS cytoplasmic male sterility
  • fertility recovery gene refers to a nuclear gene that restores cytoplasmic male sterility.
  • No fertility recovery gene means that the fertility recovery gene is deleted or does not substantially function.
  • nuclear-free means that seeds are substantially absent.
  • Substantially free of seeds means 90% or more non-nucleated, preferably 95 to 99% when the number of seeds of the male sterile maintenance line of Capsicum annuum of the present invention is 100%. It is said that it is non-nuclear, more preferably 100% non-nuclear and free of “shina”.
  • the “male sterility maintenance line” is a pollen parent line used for maintaining a cytoplasmic male sterility line, having a cytoplasmic genome that is cute and having a nuclear genome fertility restoration gene. It means no line.
  • partial result means that a fruit is formed and enlarged without fertilization.
  • Partial outcome refers to the property of fruit formation and enlargement without fertilization.
  • backcross refers to crossing again or repeatedly one of the two lines (repetitive parent) as a progeny of the two lines.
  • deposit number refers to the patent biological deposit center of the National Institute of Advanced Industrial Science and Technology (Aichi 1-chome, Tsukuba City, Ibaraki, Japan 1 6 This is a number given from postal code 305-8565).
  • cultivar “AN69” and “KY111” are available from Yokohama Ueki Co., Ltd. Kikugawa Research Farm (2265 Kamo, Kikukawa, Shizuoka, Japan, postal code 439-0031).
  • the variety “AN69” is Capsicum annuum cultivated at Yokohama Ueki Co., Ltd.
  • the variety “KY111” is a succeeding fixed system of Capsicum Annuum “Kyo Yutaka” marketed in Japan.
  • Cultivar "CMS KY111” was crossed with sweet pepper obtained from the Japanese market, and when male sterility was expressed in one strain, cultivar "KY111” was backcrossed and grown. Is.
  • Variety “NAT” is obtained by repeatedly fixing self-breeding from large bell peppers purchased in the South American market.
  • the variety “CMS NAT” is obtained by crossing the variety “NAT” with the cultivated variety “CMS KY111” and then performing backcrossing and fixing.
  • This specification includes the contents described in the specification and / or drawings of Japanese Patent Application No. 2009-051685 which is the basis of the priority of the present application.
  • FIG. 1 shows the appearance of Capsicum annuum “CMS KY111” fruit.
  • FIG. 2 shows a longitudinal section of the fruit of Capsicum annuum “CMS KY111”.
  • FIG. 3 shows the appearance of the fruit of Capsicum annuum “CMS NAT”.
  • FIG. 4 shows a longitudinal cut surface of the fruit of Capsicum annuum “CMS NAT”.
  • FIG. 5 shows the appearance of the fruit of Capsicum annuum “CMS NAT”.
  • FIG. 6 shows the appearance of capsicum annuum “CMS NAT” (left) and capsicum annuum “NAT” (right).
  • FIG. 1 shows the appearance of Capsicum annuum “CMS KY111” fruit.
  • FIG. 2 shows a longitudinal section of the fruit of Capsicum annuum “CMS KY111”.
  • FIG. 3 shows the appearance of the fruit of Capsicum annuum “CMS NAT”.
  • FIG. 7 shows the longitudinal sections of the fruits of Capsicum annuum “CMS NAT” (two left) and Capsicum annuum “NAT” (two right).
  • FIG. 8 shows the transverse cut surfaces of the fruits of Capsicum annuum “CMS NAT” and Capsicum annuum “NAT”.
  • the upper left is the upper half of the “CMS NAT” fruit
  • the lower left is the lower half of the “CMS NAT” fruit
  • the upper right is the upper half of the “NAT” fruit
  • the lower right is the lower half of the “NAT” fruit.
  • Capsicum Annuum “CMS KY111” The Capsicum Annuum “CMS KY111” is produced by multiplying the Capsicum Annuum varieties “AN69” and “KY111” and backcrossing “KY111”. “AN69” is considered to be cytoplasmic male sterile, and “KY111” is male dainty and has no fertility recovery gene. It is preferable to perform backcrossing four times or more. “CMS KY111” does not form a stamen, and a non-nucleated fruit is obtained as a result of partion. There is no “Shina” in the fruit.
  • FIG. 1 shows the appearance of “CMS KY111” fruit.
  • FIG. 2 shows a vertically cut fruit of “CMS KY111”.
  • CMS KY111 The seeds of “CMS KY111” are obtained by crossing using “CMS KY111” as a parent and “KY111”, a male sterile maintenance line, as a father. 2.
  • Method for producing CMS-type Capsicum annuum CMS-type KY111” or “CMS-type NAT” is used as a parent, and capsicum annuum, which is male and has no fertility recovery gene, is used as a father.
  • a new CMS type capsicum annuum can be obtained.
  • the backcrossing is preferably performed a sufficient number of times for stably expressing the characteristics of the father. For example, it is preferable to perform backcrossing four times or more.
  • the father may be any capsicum annuum as long as it is male and has no fertility recovery gene, such as pepper, paprika, chili pepper, and shishito.
  • paprika is selected as the father's book and the capsicum annuum obtained through backcrossing is a partly result.
  • capsicum is selected as the father's book and use the capsicum annuum obtained through backcrossing as a part-time result. Seeds can be obtained by multiplying Capsicum annuum used as the father's book as a male sterility maintenance line.
  • Capsicum annuum produced using “CMS line KY111” or “CMS line NAT” as a parent can be propagated by vegetative propagation or seed propagation.
  • the method of vegetative propagation is not particularly limited as long as Capsicum annuum can be propagated.
  • tissue culture organ culture, shoot apical culture, embryo culture, protoplast culture, etc.
  • tissue culture see, for example, Asakura-Arnold Biology 11 “Plant tissue culture”, Eizo Maeda, Asakura Shoten (The Institute of Biology's Studies in Biology no. 65, Plant Tissue Culture N. B. 1976, Edward Arnold Ltd.) and the like.
  • CMS type capsicum annuum obtained by the above production method has, for example, no pollen, no seed formation, high participatory results, and the father or mother It is preferably selected based on the fact that it is at least as large as the fruit of the capsicum annuum and has a well-balanced shape. For example, backcrossing with Capsicum Annuum “CMS KY111” as the parent and Capsicum Annuum “NAT” as the parent, “CMS NAT” is created. “NAT” is male and has no fertility recovery gene.
  • CMS-based NAT does not form stamens, and produces partless results as partless results. There is no “Shina” in the fruit. Moreover, when compared with “CMS KY111”, the result of part-effect is high, the fruit is larger, and the shape is good.
  • FIG. 3 shows the appearance of “CMS NAT” fruit.
  • FIG. 4 shows the fruit of “CMS NAT” cut vertically.
  • FIG. 5 shows the appearance of “CMS NAT” fruit.
  • FIG. 6 shows the appearance of fruits of “CMS NAT” (left) and “NAT” (right).
  • FIG. 7 shows a vertically cut fruit of “CMS NAT” and “NAT”.
  • the two on the left are “CMS NAT” and the two on the right are “NAT”.
  • FIG. 8 shows the “CMS NAT” and “NAT” fruits cut horizontally.
  • Upper left is the fruit side of "CMS NAT” fruit
  • lower left is the fruit side of "CMS NAT” fruit
  • upper right is the fruit side of "NAT” fruit
  • lower right is the bottom side of “NAT” fruit .
  • “CMS NAT” in FIGS. 5 to 8 is obtained by crossing “NAT” with “CMS KY111” and performing backcrossing 5 times thereafter.
  • CMS NAT The seed of “CMS NAT” is obtained by crossing using “CMS NAT” as a parent and “NAT” which is a male sterile maintenance line as a parent.
  • NAT a male sterile maintenance line
  • CMS NAT has better part-results and hypertrophy than “CMS KY111”, and is larger than the “CMS KY111” non-nucleated fruit, resulting in a well-shaped non-nucleated fruit.
  • Capsicum annuum of the present invention provides a stable and nucleusless fruit.
  • the work of removing the seeds becomes unnecessary, and waste can be reduced.
  • the whole fruit can be used for cooking such as stuffing peppers.
  • seeds are not formed, the burden on the plant body is small, so that many fruits can be harvested.
  • the capsicum annuum of the present invention is used for breeding as a mother book, a new variety can be produced.

Abstract

Disclosed is seedless Capsicum annuum L. Also disclosed is a method for producing the seedless Capsicum annuum L. Specifically disclosed is Capsicum annuum L. "CMS-line KY111" (Accession No. FERM BP-11223) or "CMS-line NAT" (Accession No. FERM BP-11225", which is cytoplasmically male-sterile and does not have a fertility restoring gene. Also specifically disclosed is a method for producing a line of Capsicum annuum L. which is cytoplasmically male-sterile and does not have a fertility restoring gene. The method comprises carrying out backcross breeding using "CMS-line KY111" or "CMS-line NAT" as a maternal line and a line of Capsicum annuum L. which is male-fertile and does not have a fertility restoring gene as a paternal line.

Description

無核のカプシクム・アンヌームおよびその作出方法Nucleusless Capsicum Annuum and its production method
　本発明は、無核のカプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ　Ｌ．）およびその作出方法に関する。 The present invention relates to a nuclear-free capsicum annuum (L.) and a method for producing the same.
　従来から、商品価値の向上や加工処理の効率化等を図るため、無核の作物が育成されている。
　例えば、無核のトマトが開発されている（特許文献１）。該トマトは、単為結果遺伝子を含む雄性不稔性のトマト株を母本とし、単為結果遺伝子を含むトマト株を父本として、両者を交雑することにより作出される。また、これとは別に、無核のトマトは、トマトトーン（登録商標、石原産業株式会社）などの薬剤を用いて、容易に生産することができる。
　ナスでは、細胞質雄性不稔性を利用した、無核のナス品種が育成されつつある（非特許文献１）。
　ピーマン（カプシクム・アンヌーム）では、トマトトーンのような、無核のピーマンを生産するために一般的に用いられる薬剤は、今のところ存在していない。また、雄性不稔性のピーマンは従来から存在していたが、該ピーマンを母本として、他のピーマンを父本として交雑した場合、Ｆ１に花粉ができることが多い。Ｆ１で花粉ができなかったとしても、何度も戻し交雑するうちに、花粉ができて雄性不稔性が回復する。冬期には、低温や日照不足によるストレス等により、異常な花器が形成されて受粉できず、単為結果により無核のピーマンが発生することがある。しかし、そのようなピーマンは石実と呼ばれ、硬く小型で、商品価値が低い。従って、商品価値が高く、単為結果性が高い、無核のピーマンの安定した作出が求められている。 Conventionally, nuclear-free crops have been cultivated in order to improve the commercial value and increase the efficiency of processing.
For example, a nuclear-free tomato has been developed (Patent Document 1). The tomato is produced by crossing both a male-sterile tomato strain containing a partheno-result gene as a parent and a tomato strain containing a part-effect gene as a parent. Apart from this, non-nucleated tomatoes can be easily produced using a chemical such as Tomato Tone (registered trademark, Ishihara Sangyo Co., Ltd.).
In eggplant, an anuclear eggplant variety utilizing cytoplasmic male sterility is being bred (Non-Patent Document 1).
In peppers (Kapushikum Annuum), there are currently no drugs that are commonly used to produce nuclear-free peppers, such as tomato tones. Although male sterile pepper was present conventionally the peppers as a seed parent, when crossing other peppers as the father present, can often pollen F 1. Even I could not pollen in the F 1, while the backcross many times, male sterility and be pollen is restored. In winter, due to low temperature and stress due to lack of sunshine, abnormal vases are formed and cannot pollinate, and a partless result may produce a nuclear-free pepper. However, such bell peppers are called stone fruits, are hard and small, and have a low commercial value. Accordingly, there is a demand for stable production of a nucleusless pepper with high commercial value and high participatory result.
特表２００１−５２０８６３号Special table 2001-520863
　本発明の課題は、無核のカプシクム・アンヌームおよびその作出方法を提供することである。
　本発明者は、上記課題を解決すべく鋭意研究を重ねた結果、育種中のカプシクム・アンヌームの中に細胞質雄性不稔性の系統を見出し、該系統のカプシクム・アンヌームに種々の品種のカプシクム・アンヌームを交雑し、無核のカプシクム・アンヌームを安定して得ることに成功した。
　すなわち、本発明は以下の発明を包含する。
〔１〕　細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ　Ｌ．）「ＣＭＳ系ＫＹ１１１」（受託番号ＦＥＲＭ　ＢＰ−１１２２３）。
〔２〕　細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ　Ｌ．）「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）。
〔３〕　〔１〕に記載のカプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」（受託番号ＦＥＲＭ　ＢＰ−１１２２３）または〔２〕に記載のカプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）を母本として、雄性可稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌームを父本として、戻し交雑することを含む、細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌームの作出方法。
　〔４〕　栄養繁殖または種子繁殖で増殖することをさらに含む、〔３〕に記載の方法。
　〔５〕　父本がカプシクム・アンヌーム「ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２４）である、〔３〕または〔４〕に記載の方法。
　〔６〕　〔３〕~〔５〕のいずれかに記載の方法で作出された、細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム。
　〔７〕　「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）である、〔６〕に記載のカプシクム・アンヌーム。
　〔８〕　〔６〕または〔７〕に記載のカプシクム・アンヌームの単為結果により得られた、カプシクム・アンヌームの果実。
　本明細書において、「カプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ　Ｌ．）」は、ナス科の、いわゆるピーマン、パプリカ、トウガラシ、シシトウなどの栽培種を含む。また、特にことわらない限り、「カプシクム・アンヌーム」は、植物体、種子、果実、および栄養繁殖または種子繁殖により増殖したものを含む。
　本明細書において、「細胞質雄性不稔性」（ＣＭＳ）とは、雄蘂が欠如したり、機能的な花粉が作られない雄性不稔のうち、細胞質遺伝を行う雄性不稔の性質をいう。
　本明細書において、「稔性回復遺伝子」とは、細胞質雄性不稔性を回復させる核遺伝子をいう。「稔性回復遺伝子を持たない」とは、稔性回復遺伝子が欠失しているか、実質的に機能しないことをいう。
　本明細書において、「無核」とは、種子が実質的に無いことをいう。「種子が実質的に無い」とは、本発明のカプシクム・アンヌームの雄性不稔維持系統の果実の種子の数を１００％とした場合に、９０％以上の無核、好ましくは９５~９９％の無核、より好ましくは１００％の無核でかつ「しいな」が無いことをいう。
　本明細書において、「雄性不稔維持系統」とは、細胞質雄性不稔系統を維持するために用いられる花粉親系統であって、細胞質ゲノムが可稔型で核ゲノムの稔性回復遺伝子を持たない系統をいう。
　本明細書において、「単為結果」とは、受精をしなくても果実が形成、肥大することをいう。また、「単為結果性」とは、受精をしなくても果実が形成、肥大する性質をいう。
　本明細書において、「戻し交雑」とは、２つの系統の交雑において、交雑後代に、２つの系統の片方（反復親）を、再びあるいは繰り返し交雑することをいう。
　本明細書において、「受託番号」とは、本発明のカプシクム・アンヌームの種子の寄託により、独立行政法人産業技術総合研究所　特許生物寄託センター（日本国茨城県つくば市東１丁目１番地１　中央第６　郵便番号　３０５−８５６６）から付与された番号をいう。本明細書に記載されたカプシクム・アンヌームの品種「ＣＭＳ系ＫＹ１１１」の種子（受託番号ＦＥＲＭ　ＢＰ−１１２２３、受託日２００９年２月３日）については、独立行政法人産業技術総合研究所　特許生物寄託センターから入手可能である。該種子は、受託日が２００９年２月３日であるＦＥＲＭ　Ｐ−２１７６４の国内寄託が国際寄託に移管されたものである。また、品種「ＣＭＳ系ＮＡＴ」の種子（受託番号ＦＥＲＭ　ＢＰ−１１２２５、受託日２０１０年１月１５日）および品種「ＮＡＴ」の種子（受託番号ＦＥＲＭ　ＢＰ−１１２２４、受託日２０１０年１月１５日）についても、独立行政法人産業技術総合研究所　特許生物寄託センターから入手可能である。品種「ＡＮ６９」の種子、および「ＫＹ１１１」の種子については、横浜植木株式会社　菊川研究農場（日本国静岡県菊川市加茂２２６５　郵便番号４３９−００３１）から入手可能である。品種「ＡＮ６９」は、横浜植木株式会社で栽培されていたカプシクム・アンヌームである。品種「ＫＹ１１１」は、日本国内で市販されているカプシクム・アンヌーム「京ゆたか」の後代固定系である。品種「ＣＭＳ系ＫＹ１１１」は、日本国内のマーケットより入手した甘トウガラシに品種「ＫＹ１１１」を交雑したところ、１株に雄性不稔が発現し、以後、品種「ＫＹ１１１」を戻し交雑し、育成したものである。品種「ＮＡＴ」は、南米の市場にて購入した大型ピーマンより採種したものを、自殖を繰り返し固定したものである。品種「ＣＭＳ系ＮＡＴ」は、品種「ＮＡＴ」を育成済みの品種「ＣＭＳ系ＫＹ１１１」に交雑し、以後、戻し交雑を行い固定したものである。
　本明細書は本願の優先権の基礎である日本国特許出願２００９−０５１６８５号の明細書および／または図面に記載される内容を包含する。 An object of the present invention is to provide a nuclear-free capsicum annuum and a method for producing the same.
As a result of intensive studies to solve the above problems, the present inventor found a cytoplasmic male sterile line in capsicum annuum during breeding, and various varieties of capsicum annuum in the line of capsicum annuum. Crossed the Annuum and succeeded in obtaining a stable and non-nuclear Capsicum Annuum.
That is, the present invention includes the following inventions.
[1] Capsicum annuum L. “CMS system KY111” (accession number FERM BP-11223) which is cytoplasmic male sterile and does not have a fertility recovery gene.
[2] Capsicum annuum L. “CMS NAT” (accession number FERM BP-11225), which is cytoplasmic male sterile and does not have a fertility recovery gene.
[3] Capsicum annuum “CMS KY111” (accession number FERM BP-11223) according to [1] or Capsicum annuum “CMS NAT” (accession number FERM BP-11225) according to [2] As a book, the production of capsicum annuum that is male sterile and does not have the fertility recovery gene, including backcrossing with Capsicum annuum that is male fragile and has no fertility recovery gene Method.
[4] The method according to [3], further comprising growing by vegetative propagation or seed propagation.
[5] The method according to [3] or [4], wherein the parent book is Capsicum annuum “NAT” (Accession No. FERM BP-11224).
[6] Capsicum annuum produced by the method according to any one of [3] to [5] and having no cytoplasmic male sterility and no fertility recovery gene.
[7] Capsicum annuum according to [6], which is “CMS NAT” (accession number FERM BP-11225).
[8] A fruit of Capsicum annuum obtained as a result of participation of Capsicum annuum according to [6] or [7].
In this specification, "Capsicum annuum (Capsicum annuum L.)" includes cultivated species such as so-called bell peppers, paprika, chili pepper, and shishitou. In addition, unless otherwise specified, “Kapushikum annuum” includes plants, seeds, fruits, and those grown by vegetative propagation or seed propagation.
As used herein, “cytoplasmic male sterility” (CMS) refers to the male sterility of cytoplasmic inheritance among male sterility that lacks stamens or does not produce functional pollen. .
As used herein, “fertility recovery gene” refers to a nuclear gene that restores cytoplasmic male sterility. “No fertility recovery gene” means that the fertility recovery gene is deleted or does not substantially function.
In the present specification, “nuclear-free” means that seeds are substantially absent. “Substantially free of seeds” means 90% or more non-nucleated, preferably 95 to 99% when the number of seeds of the male sterile maintenance line of Capsicum annuum of the present invention is 100%. It is said that it is non-nuclear, more preferably 100% non-nuclear and free of “shina”.
In the present specification, the “male sterility maintenance line” is a pollen parent line used for maintaining a cytoplasmic male sterility line, having a cytoplasmic genome that is cute and having a nuclear genome fertility restoration gene. It means no line.
In the present specification, the “partial result” means that a fruit is formed and enlarged without fertilization. “Partial outcome” refers to the property of fruit formation and enlargement without fertilization.
In the present specification, “backcross” refers to crossing again or repeatedly one of the two lines (repetitive parent) as a progeny of the two lines.
In the present specification, the “deposit number” refers to the patent biological deposit center of the National Institute of Advanced Industrial Science and Technology (Aichi 1-chome, Tsukuba City, Ibaraki, Japan 1 6 This is a number given from postal code 305-8565). Regarding the seeds of the cultivar “CMS KY111” of Capsicum Annuum described in this specification (accession number FERM BP-11223, date of accession February 3, 2009), the National Institute of Advanced Industrial Science and Technology patent biological deposit It is available from the center. The seeds are obtained by transferring the domestic deposit of FERM P-2164, whose deposit date is February 3, 2009, to the international deposit. Also, seeds of cultivar “CMS NAT” (accession number FERM BP-11225, accession date January 15, 2010) and seeds of cultivar “NAT” (accession number FERM BP-11224, accession date January 15, 2010). ) Can also be obtained from the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology. Seeds of cultivar “AN69” and “KY111” are available from Yokohama Ueki Co., Ltd. Kikugawa Research Farm (2265 Kamo, Kikukawa, Shizuoka, Japan, postal code 439-0031). The variety “AN69” is Capsicum annuum cultivated at Yokohama Ueki Co., Ltd. The variety “KY111” is a succeeding fixed system of Capsicum Annuum “Kyo Yutaka” marketed in Japan. Cultivar "CMS KY111" was crossed with sweet pepper obtained from the Japanese market, and when male sterility was expressed in one strain, cultivar "KY111" was backcrossed and grown. Is. Variety “NAT” is obtained by repeatedly fixing self-breeding from large bell peppers purchased in the South American market. The variety “CMS NAT” is obtained by crossing the variety “NAT” with the cultivated variety “CMS KY111” and then performing backcrossing and fixing.
This specification includes the contents described in the specification and / or drawings of Japanese Patent Application No. 2009-051685 which is the basis of the priority of the present application.
　図１は、カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」の果実の外観を示す。
　図２は、カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」の果実の縦切断面を示す。
　図３は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」の果実の外観を示す。
　図４は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」の果実の縦切断面を示す。
　図５は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」の果実の外観を示す。
　図６は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」（左）とカプシクム・アンヌーム「ＮＡＴ」（右）の果実の外観を示す。
　図７は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」（左２つ）とカプシクム・アンヌーム「ＮＡＴ」（右２つ）の果実の縦切断面を示す。
　図８は、カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」とカプシクム・アンヌーム「ＮＡＴ」の果実の横切断面を示す。左上は「ＣＭＳ系ＮＡＴ」の果実の上半分、左下は「ＣＭＳ系ＮＡＴ」の果実の下半分、右上は「ＮＡＴ」の果実の上半分、右下は「ＮＡＴ」の果実の下半分である。 FIG. 1 shows the appearance of Capsicum annuum “CMS KY111” fruit.
FIG. 2 shows a longitudinal section of the fruit of Capsicum annuum “CMS KY111”.
FIG. 3 shows the appearance of the fruit of Capsicum annuum “CMS NAT”.
FIG. 4 shows a longitudinal cut surface of the fruit of Capsicum annuum “CMS NAT”.
FIG. 5 shows the appearance of the fruit of Capsicum annuum “CMS NAT”.
FIG. 6 shows the appearance of capsicum annuum “CMS NAT” (left) and capsicum annuum “NAT” (right).
FIG. 7 shows the longitudinal sections of the fruits of Capsicum annuum “CMS NAT” (two left) and Capsicum annuum “NAT” (two right).
FIG. 8 shows the transverse cut surfaces of the fruits of Capsicum annuum “CMS NAT” and Capsicum annuum “NAT”. The upper left is the upper half of the “CMS NAT” fruit, the lower left is the lower half of the “CMS NAT” fruit, the upper right is the upper half of the “NAT” fruit, and the lower right is the lower half of the “NAT” fruit. .
１．カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」
　カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」は、カプシクム・アンヌームの品種「ＡＮ６９」と「ＫＹ１１１」を掛け合わせ、「ＫＹ１１１」を戻し交雑することにより作出される。「ＡＮ６９」は、細胞質雄性不稔性であると考えられ、「ＫＹ１１１」は、雄性可稔性であり、かつ稔性回復遺伝子を持たない。戻し交雑は４回以上行うことが好ましい。
　「ＣＭＳ系ＫＹ１１１」は、雄蘂を形成せず、単為結果により無核の果実が得られる。該果実には「しいな」も見られない。図１に、「ＣＭＳ系ＫＹ１１１」の果実がなっている様子を示す。図２に、「ＣＭＳ系ＫＹ１１１」の果実を縦に切断したものを示す。
　「ＣＭＳ系ＫＹ１１１」の種子は、「ＣＭＳ系ＫＹ１１１」を母本とし、雄性不稔維持系統である「ＫＹ１１１」を父本として交雑することによって得られる。
２．ＣＭＳ系カプシクム・アンヌームの作出方法
　カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」または「ＣＭＳ系ＮＡＴ」を母本として、雄性可稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌームを父本として、該父本を反復親として戻し交雑することにより、新規のＣＭＳ系カプシクム・アンヌームを得ることができる。戻し交雑は、父本の特性を安定して発現させるための十分な回数を行うことが好ましい。例えば、戻し交雑を４回以上行うことが好ましい。
　父本は、雄性可稔性でかつ稔性回復遺伝子を持たないものであれば、どのカプシクム・アンヌームでもよく、ピーマン、パプリカ、トウガラシ、シシトウなどが挙げられる。例えば、肉厚の無核の果実を得たい場合は、父本としてパプリカを選択し、戻し交雑を経て得られたカプシクム・アンヌームを単為結果させることが考えられる。辛味のある無核の果実を得たい場合は、父本としてトウガラシを選択し、戻し交雑を経て得られたカプシクム・アンヌームを単為結果させることが考えられる。種子は、父本として用いたカプシクム・アンヌームを雄性不稔維持系統として掛け合わせれば、得ることができる。
　「ＣＭＳ系ＫＹ１１１」または「ＣＭＳ系ＮＡＴ」を母本として作出されたカプシクム・アンヌームは、栄養繁殖または種子繁殖で増殖させることができる。栄養繁殖の方法は、カプシクム・アンヌームを増殖できれば特に限定されない。例えば、挿し木、接ぎ木、および組織培養（器官培養、茎頂培養、胚培養、プロトプラスト培養など）が挙げられる。組織培養については、例えば、Ａｓａｋｕｒａ−Ａｒｎｏｌｄ　Ｂｉｏｌｏｇｙ　１１「植物組織培養」、前田英三訳、朝倉書店（Ｔｈｅ　Ｉｎｓｔｉｔｕｔｅ　ｏｆ　Ｂｉｏｌｏｇｙ’ｓ　Ｓｔｕｄｉｅｓ　ｉｎ　Ｂｉｏｌｏｇｙ　ｎｏ．６５，Ｐｌａｎｔ　Ｔｉｓｓｕｅ　Ｃｕｌｔｕｒｅ，Ｄｅｎｎｉｓ　Ｎ．Ｂｕｔｃｈｅｒ　ｅｔ　ａｌ．，１９７６，Ｅｄｗａｒｄ　Ａｒｎｏｌｄ　Ｌｔｄ．）などを参考にして行うことができる。種子繁殖は、雄性不稔維持系統と交雑して得られた種子を播種して行うことができる。
３．上記作出方法により得られたＣＭＳ系カプシクム・アンヌーム
　上記作出方法により得られたＣＭＳ系カプシクム・アンヌームは、例えば、花粉が出ず、種子が形成されないこと、単為結果性が高く、父本または母本のカプシクム・アンヌームの果実と同等以上の大きさであり、均整のとれた形を有することなどを基準として選抜されたものであることが好ましい。
　例えば、カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」を母本として、カプシクム・アンヌーム「ＮＡＴ」を父本として、戻し交雑することにより、「ＣＭＳ系ＮＡＴ」が作出される。「ＮＡＴ」は、雄性可稔性であり、かつ稔性回復遺伝子を持たない。上述のように母本の「ＣＭＳ系ＫＹ１１１」も稔性回復遺伝子を持たないので、何度戻し交雑をしても、稔性は回復しない。戻し交雑は４回以上行うことが好ましい。
　「ＣＭＳ系ＮＡＴ」は、雄蘂を形成せず、単為結果により無核の果実が得られる。該果実には「しいな」も見られない。また、「ＣＭＳ系ＫＹ１１１」と比較すると、単為結果性が高く、果実もより大型であり、形は良好である。図３に、「ＣＭＳ系ＮＡＴ」の果実の外観を示す。図４に、「ＣＭＳ系ＮＡＴ」の果実を縦に切断したものを示す。図５に、「ＣＭＳ系ＮＡＴ」の果実がなっている様子を示す。図６に、「ＣＭＳ系ＮＡＴ」（左）と「ＮＡＴ」（右）の果実の外観を示す。図７に、「ＣＭＳ系ＮＡＴ」と「ＮＡＴ」の果実を縦に切断したものを示す。左２つが「ＣＭＳ系ＮＡＴ」、右二つが「ＮＡＴ」である。図８に、「ＣＭＳ系ＮＡＴ」と「ＮＡＴ」の果実を横に切断したものを示す。左上は「ＣＭＳ系ＮＡＴ」の果実のヘタ側、左下は「ＣＭＳ系ＮＡＴ」の果実の尻側、右上は「ＮＡＴ」の果実のヘタ側、右下は「ＮＡＴ」の果実の尻側である。なお、図５~図８の「ＣＭＳ系ＮＡＴ」は、「ＮＡＴ」を「ＣＭＳ系ＫＹ１１１」に交雑し、以後、５回の戻し交雑を行なったものである。
　「ＣＭＳ系ＮＡＴ」の種子は、「ＣＭＳ系ＮＡＴ」を母本とし、雄性不稔維持系統である「ＮＡＴ」を父本として交雑することによって得られる。
　以下、実施例によって本発明を更に具体的に説明するが、これらの実施例は本発明を何ら限定するものでない。 1. Capsicum Annuum “CMS KY111”
The Capsicum Annuum “CMS KY111” is produced by multiplying the Capsicum Annuum varieties “AN69” and “KY111” and backcrossing “KY111”. “AN69” is considered to be cytoplasmic male sterile, and “KY111” is male dainty and has no fertility recovery gene. It is preferable to perform backcrossing four times or more.
“CMS KY111” does not form a stamen, and a non-nucleated fruit is obtained as a result of partion. There is no “Shina” in the fruit. FIG. 1 shows the appearance of “CMS KY111” fruit. FIG. 2 shows a vertically cut fruit of “CMS KY111”.
The seeds of “CMS KY111” are obtained by crossing using “CMS KY111” as a parent and “KY111”, a male sterile maintenance line, as a father.
2. Method for producing CMS-type Capsicum annuum Capsicum annuum “CMS-type KY111” or “CMS-type NAT” is used as a parent, and capsicum annuum, which is male and has no fertility recovery gene, is used as a father. By backcrossing the book as a recurrent parent, a new CMS type capsicum annuum can be obtained. The backcrossing is preferably performed a sufficient number of times for stably expressing the characteristics of the father. For example, it is preferable to perform backcrossing four times or more.
The father may be any capsicum annuum as long as it is male and has no fertility recovery gene, such as pepper, paprika, chili pepper, and shishito. For example, when it is desired to obtain a thick, non-nucleated fruit, it is conceivable that paprika is selected as the father's book and the capsicum annuum obtained through backcrossing is a partly result. If you want to get a spicy, non-nucleated fruit, you can select capsicum as your father's book and use the capsicum annuum obtained through backcrossing as a part-time result. Seeds can be obtained by multiplying Capsicum annuum used as the father's book as a male sterility maintenance line.
Capsicum annuum produced using “CMS line KY111” or “CMS line NAT” as a parent can be propagated by vegetative propagation or seed propagation. The method of vegetative propagation is not particularly limited as long as Capsicum annuum can be propagated. For example, cuttings, grafts, and tissue culture (organ culture, shoot apical culture, embryo culture, protoplast culture, etc.) can be mentioned. For tissue culture, see, for example, Asakura-Arnold Biology 11 “Plant tissue culture”, Eizo Maeda, Asakura Shoten (The Institute of Biology's Studies in Biology no. 65, Plant Tissue Culture N. B. 1976, Edward Arnold Ltd.) and the like. Seed breeding can be performed by sowing seeds obtained by crossing with a male sterile line.
3. CMS type capsicum annuum obtained by the above production method The CMS type capsicum annuum obtained by the above production method has, for example, no pollen, no seed formation, high participatory results, and the father or mother It is preferably selected based on the fact that it is at least as large as the fruit of the capsicum annuum and has a well-balanced shape.
For example, backcrossing with Capsicum Annuum “CMS KY111” as the parent and Capsicum Annuum “NAT” as the parent, “CMS NAT” is created. “NAT” is male and has no fertility recovery gene. As described above, the mother “CMS KY111” also does not have a fertility recovery gene, and thus fertility does not recover even if backcrossing. It is preferable to perform backcrossing four times or more.
“CMS-based NAT” does not form stamens, and produces partless results as partless results. There is no “Shina” in the fruit. Moreover, when compared with “CMS KY111”, the result of part-effect is high, the fruit is larger, and the shape is good. FIG. 3 shows the appearance of “CMS NAT” fruit. FIG. 4 shows the fruit of “CMS NAT” cut vertically. FIG. 5 shows the appearance of “CMS NAT” fruit. FIG. 6 shows the appearance of fruits of “CMS NAT” (left) and “NAT” (right). FIG. 7 shows a vertically cut fruit of “CMS NAT” and “NAT”. The two on the left are “CMS NAT” and the two on the right are “NAT”. FIG. 8 shows the “CMS NAT” and “NAT” fruits cut horizontally. Upper left is the fruit side of "CMS NAT" fruit, lower left is the fruit side of "CMS NAT" fruit, upper right is the fruit side of "NAT" fruit, and lower right is the bottom side of "NAT" fruit . Note that “CMS NAT” in FIGS. 5 to 8 is obtained by crossing “NAT” with “CMS KY111” and performing backcrossing 5 times thereafter.
The seed of “CMS NAT” is obtained by crossing using “CMS NAT” as a parent and “NAT” which is a male sterile maintenance line as a parent.
Hereinafter, the present invention will be described more specifically with reference to examples. However, these examples do not limit the present invention.
〔カプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」の作出〕
　１９９８年に、カプシクム・アンヌーム（中型ピーマン）の「ＡＮ６９」×「ＫＹ１１１」のＦ１のすべての株が、雄性不稔となり、着果不良となった。
　「ＡＮ６９」が細胞質雄性不稔性（ＣＭＳ）である可能性があると考えられたので、「ＡＮ６９」と様々なカプシクム・アンヌームの品種とを交雑し、稔性を調査した。その結果、「ＫＹ１１１」を含む６品種とのＦ１が雄性不稔となった。また、「ＡＮ６９」の自殖および系統内交雑では、雄性可稔となり、「ＡＮ６９」は、稔性回復遺伝子を持っていると考えられた。
　以後、上記６品種を、繰り返し戻し交雑（年２回）し、花粉稔性と着果の調査を行った。「ＫＹ１１１」以外の５品種は、戻し交雑を１０回ほど行う内に途中で花粉稔性が戻り、細胞質雄性不稔性が崩れた。「ＫＹ１１１」は、稔性回復遺伝子を持たないと考えられた。
　従って、「ＡＮ６９」×「ＫＹ１１１」に「ＫＹ１１１」を戻し交雑することにより、「ＡＮ６９」の細胞質と「ＫＹ１１１」の核を持つ「ＣＭＳ系ＫＹ１１１」（受託番号ＦＥＲＭ　ＢＰ−１１２２３）を得ることができた。「ＣＭＳ系ＫＹ１１１」の単為結果により得られた果実は、無核であった。 [Production of Capsicum Annuum "CMS KY111"]
In 1998, all strains of the F 1 of "AN69" × "KY111" of Capsicum annuum (medium-sized green pepper) becomes a male sterile, became the fruit set failure.
Since it was thought that "AN69" may be cytoplasmic male sterility (CMS), "AN69" was crossed with various capsicum annuum varieties and fertility was investigated. As a result, F 1 with 6 varieties including “KY111” became male sterile. In addition, the self-breeding of “AN69” and the cross within the line resulted in male facilitation, and “AN69” was considered to have a fertility recovery gene.
Thereafter, the above six varieties were repeatedly backcrossed (twice a year) to investigate pollen fertility and fruit set. Five varieties other than “KY111” returned to pollen fertility during the course of backcrossing about 10 times, and the cytoplasmic male sterility was disrupted. “KY111” was thought to have no fertility recovery gene.
Therefore, by backcrossing “KY111” to “AN69” × “KY111”, it is possible to obtain “CMS KY111” (accession number FERM BP-11223) having the cytoplasm of “AN69” and the nucleus of “KY111”. did it. The fruit obtained by the part-result of “CMS KY111” was anucleate.
〔カプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」の作出〕
　果実の品質がより良く、単為結果性がより高い系統を作出するため、実施例１で作出した「ＣＭＳ系ＫＹ１１１」を母本として、カプシクム・アンヌームの様々な品種を父本として、組み合わせ検定を行った。検定では、安定した雄性不稔性と高い単為結果性を持つ組み合わせを模索した。
　２００４年に、横浜植木株式会社が以前より所有していた「ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２４）と組み合わせることにより、無核のカプシクム・アンヌームの果実が得られることがわかった。「ＮＡＴ」は、稔性回復遺伝子を持たないと考えられた。以後、「ＮＡＴ」を繰り返し戻し交雑して「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）を構築した。また、雄性不稔維持系統として「ＮＡＴ」を用いることにより、「ＣＭＳ系ＮＡＴ」の種子を得た。
　「ＣＭＳ系ＮＡＴ」は、「ＣＭＳ系ＫＹ１１１」よりも単為結果性、肥大性が向上しており、「ＣＭＳ系ＫＹ１１１」の無核の果実よりも大きく、形のよい無核果実が得られた（図３および図４）。 [Production of Capsicum Annuum "CMS NAT"]
In order to produce a line with better fruit quality and higher participatory results, the combination test was conducted using the “CMS KY111” produced in Example 1 as the parent and various capsicum annuum varieties as the father. Went. The test sought combinations with stable male sterility and high parthenologic outcome.
In 2004, it was found that when combined with “NAT” (Accession No. FERM BP-11224), which was previously owned by Yokohama Ueki Co., Ltd., it was possible to obtain the fruit of a nuclear-free Capsicum annum. “NAT” was thought to have no fertility recovery gene. Thereafter, “NAT” was repeatedly backcrossed to construct “CMS NAT” (Accession No. FERM BP-11225). In addition, seeds of “CMS NAT” were obtained by using “NAT” as a male sterility maintenance line.
“CMS NAT” has better part-results and hypertrophy than “CMS KY111”, and is larger than the “CMS KY111” non-nucleated fruit, resulting in a well-shaped non-nucleated fruit. (FIGS. 3 and 4).
　本発明のカプシクム・アンヌームの単為結果により、安定して無核の果実が得られる。該果実を加工する際、種子を除去する作業が不要になり、廃棄物が削減できる。ピーマンの肉詰めなどの料理の際、果実を丸ごと用いることができる。
　また、種子が形成されないことにより、植物体への負担が少ないので、果実を多く収穫し得る。
　さらに、本発明のカプシクム・アンヌームを母本として育種に用いれば、新品種を作出することができる。 The participatory result of Capsicum annuum of the present invention provides a stable and nucleusless fruit. When processing the fruits, the work of removing the seeds becomes unnecessary, and waste can be reduced. The whole fruit can be used for cooking such as stuffing peppers.
Moreover, since seeds are not formed, the burden on the plant body is small, so that many fruits can be harvested.
Furthermore, if the capsicum annuum of the present invention is used for breeding as a mother book, a new variety can be produced.
ＦＥＲＭ　ＢＰ−１１２２３
ＦＥＲＭ　ＢＰ−１１２２４
ＦＥＲＭ　ＢＰ−１１２２５
　本明細書で引用した全ての刊行物、特許および特許出願をそのまま参考として本明細書にとり入れるものとする。 FERM BP-11223
FERM BP-11224
FERM BP-11225
All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

Claims (8)

  1. 　細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ＿　Ｌ．）「ＣＭＳ系ＫＹ１１１」（受託番号ＦＥＲＭ　ＢＰ−１１２２３）。 Capsicum annuum “CMS system KY111” (accession number FERM BP-11223) which is cytoplasmic male sterile and does not have a fertility recovery gene.
  2. 　細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム（Ｃａｐｓｉｃｕｍ　ａｎｎｕｕｍ　Ｌ．）「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）。 Capsicum annuum “CMS NAT” (accession number FERM BP-11225) which is cytoplasmic male sterile and does not have a fertility recovery gene.
  3. 　請求項１に記載のカプシクム・アンヌーム「ＣＭＳ系ＫＹ１１１」（受託番号ＦＥＲＭ　ＢＰ−１１２２３）または請求項２に記載のカプシクム・アンヌーム「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）を母本として、雄性可稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌームを父本として、戻し交雑することを含む、細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌームの作出方法。 Capsicum annuum “CMS system KY111” (Accession number FERM BP-11223) according to claim 1 or Capsicum annuum “CMS system NAT” (Accession number FERM BP-11225) according to claim 2 A method for producing Capsicum annuum that is cytoplasmic male sterile and does not have a fertility recovery gene, including backcrossing with Capsicum annuum that is male and has no fertility recovery gene as a parent.
  4. 　栄養繁殖または種子繁殖で増殖することをさらに含む、請求項３に記載の方法。 4. The method of claim 3, further comprising growing by vegetative propagation or seed propagation.
  5. 　父本がカプシクム・アンヌーム「ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２４）である、請求項３または４に記載の方法。 The method according to claim 3 or 4, wherein the father's book is Capsicum Annuum "NAT" (Accession Number FERM BP-11224).
  6. 　請求項３~５のいずれか１項に記載の方法で作出された、細胞質雄性不稔性でかつ稔性回復遺伝子を持たないカプシクム・アンヌーム。 Capsicum annuum produced by the method according to any one of claims 3 to 5 and having no cytoplasmic male sterility and no fertility recovery gene.
  7. 　「ＣＭＳ系ＮＡＴ」（受託番号ＦＥＲＭ　ＢＰ−１１２２５）である、請求項６に記載のカプシクム・アンヌーム。 The capsicum annuum according to claim 6, which is “CMS NAT” (accession number FERM BP-11225).
  8. 　請求項６または７に記載のカプシクム・アンヌームの単為結果により得られた、カプシクム・アンヌームの果実。 The fruit of Capsicum annuum obtained as a result of participation of Capsicum annuum according to claim 6 or 7.
PCT/JP2010/053731 2009-03-05 2010-03-02 Seedless capsicum annuum l. and method for producing same WO2010101274A1 (en)

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WO2011085216A2 (en) 2010-01-08 2011-07-14 Ironwood Pharmaceuticals, Inc. Use of faah inhibitors for treating parkinson's disease and restless legs syndrome
WO2011123719A2 (en) 2010-03-31 2011-10-06 Ironwood Pharmaceuticals, Inc. Use of faah inhibitors for treating abdominal, visceral and pelvic pain
US9253953B2 (en) 2010-12-22 2016-02-09 Rijk Zwaan Zaadteelt En Zaadhandel B.V Fruit formation in the absence of fertilisation
WO2012087140A3 (en) * 2010-12-22 2012-09-20 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Fruit formation in the absence of fertilisation
CN103501592A (en) * 2010-12-22 2014-01-08 瑞克斯旺种苗集团公司 Fruit formation in the absence of fertilisation
NL2005908C2 (en) * 2010-12-22 2012-06-28 Rijk Zwaan Zaadteelt En Zaadhandel Bv Fruit formation in the absence of fertilisation.
AU2011345435B2 (en) * 2010-12-22 2017-02-23 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Fruit formation in the absence of fertilisation
CN107278872A (en) * 2010-12-22 2017-10-24 瑞克斯旺种苗集团公司 Formed in the absence of the fruit in the case of fertilization
US9642318B2 (en) 2011-11-22 2017-05-09 Seminis Vegetable Seeds, Inc. Seedless pepper plants
US10499578B2 (en) 2011-11-22 2019-12-10 Seminis Vegetable Seeds, Inc. Seedless pepper plants
US11091773B2 (en) 2014-01-17 2021-08-17 National Research And Development Agency National Agriculture And Food Research Organization Parthenocarpy regulation gene and use thereof
CN104206266A (en) * 2014-08-25 2014-12-17 镇江瑞繁农艺有限公司 Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line
CN111406644A (en) * 2020-04-20 2020-07-14 山西省农业科学院蔬菜研究所 Breeding method of nucleus-cytoplasm interactive male sterile line of cluster pod pepper and application of three-line matching system
CN111406644B (en) * 2020-04-20 2023-05-30 山西农业大学 Cluster-growing pod pepper nuclear-cytoplasmic interaction type male sterile line breeding method and application of three-line matched system

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