CN106962181A - A kind of method of integrated cultural hybrid rice odor type restorer - Google Patents
A kind of method of integrated cultural hybrid rice odor type restorer Download PDFInfo
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Abstract
The invention discloses a kind of method of integrated cultural hybrid rice odor type restorer, the method of integrated cultural hybrid rice odor type restorer is hybridized using geographical distant race breeding parent material, integrated use " four is early synchronous " multi-environment orientation stress breeding technique and method, cultivating to obtain has fragrance, high-combining ability, strong restoring force, the restorer that rice quality is excellent, comprehensive agronomy character is excellent and rice blast resistance is strong；The synchronous improvement of quality, yield and resistance, the Hybrid Aromatic Rice restorer that breeding goal character is prominent, Comprehensive Traits are excellent are realized using the hybridization between high-quality × high-quality, high-quality × breeding for disease resistance parent material.Integrated use of the present invention " four is early synchronous " multi-environment orientation stress breeding technique and method, can make whole spice type Hybrid Rice Combination by authorization, the whole spice type kind main quality index of incubation up to 3 grades of national standard and more than, savory, rice blast resistance is stronger.
Description
Technical field
The invention belongs to rice breeding technology field, more particularly to a kind of side of integrated cultural hybrid rice odor type restorer
Method.
Background technology
Grain security is global major issue.The development of China's Rice Production must walk high-quality, high yield and disease-resistant organic knot
Close, the road of coordinated development.The nineties in last century, it is not preceding universal with rice quality that China's hybrid rice production faces low yield
Deviation two is challenged greatly, and it is that breeding material hereditary basis is narrow to study carefully its main cause, and coordinate force does not have big breakthrough.Hybrid rice is real
Matter is that, using the hybrid vigour between sterile line and restorer, the key of breeding success or failure is the innovation and improvement of material.Such as me
Bright extensive 63 seed selection of the foremost restorer of state and the application of Shanyou 63 have promoted the significant development of China's series of three-series hybrid rice.It
High-grade rice restorer polyphyly 1, another name for Sichuan Province extensive 527, CDR22, Mianhui725 and the successful application of combination, have continued to press on China afterwards
The development of quality breeding in three-line hybrid rice, breeding for disease resistance, but the high-grade high-quality being had long enjoyed a good reputation in terms of rice quality with the world
Rice THAI Fragrant rice compares, and also has very big gap, far can not also meet the demand of market and consumer to rice quality.
The reason for further analysis hybrid paddy rice lacks high-grade superior rice, its one of the main reasons is female parent of hybrid rice sterile line
Caused by poor quality, another reason also lacks the hybrid paddy rice male parent (restorer) of quality breakthrough.Although new restorer line seed selection is successful
Probability is far above new sterile line, but real large-area applications are in the few of production, main cause be these restorers only quality,
Some or certain two characters are more prominent in the characters such as coordinate force, blast resisting characteristic, polymerize the breakthrough of multiple merits
Odor type restorer is even more rare.Simultaneously also neither one it is effective cultivate High quality and diseases resistance Hybrid Aromatic Rice restorer method.
The main method of existing seed selection restorer is pedigree method, and most breeding units are in restorer filial generation menu strain to F5, F6 generation
The basicly stable ability test cross sterile line of colony, and it is few in the means and method of Comprehensive Control seed selection, there is seed selection restorer
Year limit for length, single traits is protruded, and comprehensive merit polymerization is inadequate, and the defects such as large-area applications are unable in production.
In summary, there is seed selection year limit for length in the disease-resistant system, method that recovers of existing seed selection high-quality odor type, and single traits is protruded, comprehensive
Close merit polymerization inadequate, the problem of being unable to large-area applications in production.
The content of the invention
It is an object of the invention to provide a kind of method of integrated cultural hybrid rice odor type restorer, it is intended to solves existing
Seed selection high-quality odor type is disease-resistant to recover the year limit for length that system, method has seed selection, and single traits is protruded, and comprehensive merit polymerization is inadequate,
The problem of large-area applications being unable in production.
To achieve the above object, the technical solution adopted by the present invention is：A kind of integrated cultural hybrid rice odor type restorer
Method, step includes：
S101 is using advantage of the geographical distant hybridization to hybrid, with four early synchronous multi-environment orientation stress selection choosings
Educate high yield coordinate force restorer；
S102：Scent gene and middle low amylose content gene are imported using " high-quality × high-quality ", and in the high temperature dry season
Under the conditions of stress M8003 line low chalkness ratio, low chalkiness degree and high head rice rate；
S103：Resistant gene is imported using " high-quality × disease-resistant ", is combined by artificial infection and spontaneous induction and screens disease-resistant
The strong strain of property；The degeneration-resistant Sexual behavior mode of high- and low-temperature resistance is carried out using nature high/low temperature；
S104：Plant and leaf shape improvement is carried out using character complementation, battalion is improved with proper extension breeding time, increase plant height
Health is long-term and biological yield；
S105：On Spike-grain structure, appropriateness increase grain weight, grain number per spike is taken into account again with grain, and storehouse source stream is coordinated to solve kind
Stable high yield.
Further, it is material of the selection with the fragrant glutinous relationship in Yunnan or its hybridization that the high yield coordinate force restorer, which is cultivated,
Intermediate materials hybridize with excellent backbone parent material, reestablish diplomatic relations.
Further, in S101, described four early synchronous multi-environment orientation stress selection steps are：
1. rice quality is set up early for screening and assessment system：Mutually tied with strange land, field and indoor seed selection from early Dai Zheng seasons
Close and carry out early for Quality Identification；
2. disease-resistant degeneration-resistant morning is set up for characterization and evaluation；
3. fragrance is set up early for characterization and evaluation；
4. set up early for the excellent evaluation of test cross survey：Selection standard sterile line does manual test cross F1 kinds since the generation of selfing 2,3 generations,
Roguing system hybrid vigour is screened in progress, increases coordinate force seed selection dynamics；Standard sterile line is selected：Zhenshan 97a, II -32A, ridge
Multiple well-known sterile lines such as 46A, interior fragrant A make Testers.
Further, 1., set up rice quality early includes step for screening and assessment system：Exterior quality Screening and Identification：Even
In continuous generation more than 3 years, selects fragrance individual plant to be ground into brown rice with going out rough machine indoors in Sichuan inland river, Lingshui pair, Hainan, selects outward appearance complete
Whole, no chalk is white or substantially white without chalk, the good brown rice sowing of transparency.
Further, 2., set up disease-resistant degeneration-resistant morning includes step for characterization and evaluation：The early generation identification of rice blast resistance, from morning
For continuous 3 years of segregating generation selection exterior quality is excellent, dulcet fine individual plant carries out many fungus strain mixing artificial infections of rice blast
Identification, the individual plant of orientation stress selection resistance；The sowing of material selection, completes 4 people mid-October in annual completion preceding by the end of September
Work inoculated identification, mid or late November completes blast resisting identification, obtains the consistent material of resistance；
The early generation identification of high- and low-temperature resistance resistance, using the high temperature dry season in the first tenday period of a month inland river late July 8, in orientation stress screening
Roguing system discolors quality for index to the endurance of high temperature with colony when the height of setting percentage and maturation；Utilize rice blast resistance
Roguing system seedling stage is removed in the natural low temperature orientation stress screening of greenhouse after having identified to the endurance of low temperature, in mirror of same time
It is fixed, using the withered degree size of colony as index.
Further, 3., set up fragrance early includes step for characterization and evaluation：The tender seedling rubbing method in field, the grain yellow maturity grain of rice
Chew-in technique and laboratory KOH solution infusion method identification fragrance；From extensive cross combination early selfing line segregating generation continuous 3 generation is made in seedling
Phase, maturity period selection fine individual plant carry out fragrance Screening and Identification, and dulcet strain is target strain.
Further, 4. step, sets up early for the excellent assessment process of test cross survey：Opened from extensive combination selfing 2 generations of separation, 3 generations are made
Beginning selection standard sterile line does manual test cross F1 kinds, and roguing system hybrid vigour is screened in progress, increases coordinate force seed selection dynamics；Again
Select the apolegamy whole spice type high-quality scented rice combination of odor type sterile line.
Further, the one or more during the standard sterile line selection is following：It is Zhenshan 97a, II -32A, Gang No.46A, interior
Fragrant A.
The method have the benefit that：The method for the integrated cultural hybrid rice odor type restorer that the present invention is provided,
Hybridized using geographical distant race breeding parent material, it is fixed that novelty establishes hybrid rice odor type restorer " four is early synchronous " multi-environment
To stress breeding technique and method, by carrying out rice quality, fragrance, disease-resistant degeneration-resistant and survey advantage in early generation (F2), with reference to height
Warm dry season Quality Identification, Pyricularia oryzae inoculation resistance screening, natural low temperature Evaluation of Cold Tolerance, the different season adaptability identification in strange land, field
Between the multi-environment orientation stress selection technique such as polymerize with indoor comprehensive character, cultivate that objective trait is prominent, Comprehensive Traits are excellent
The method of odor type restorer；Specifically, material of the selection containing the fragrant glutinous relationship of Yunnan Guanyuan or its hybridize intermediate materials with it is excellent
Good backbone parent material hybridization, reestablishes diplomatic relations.Rice quality is synchronously carried out from restorer selfing F2 generations (early generation), it is fragrance, disease-resistant degeneration-resistant
With the identification of the advantage of survey, the increase stress selection pressure under multi-environment, orientation carries out many excellent genes polymerizations, how excellent economical character
Selection, cultivates the restorer of " two high one excellent one is fragrant " (high-combining ability, high resistant to rice blast, high-quality, fragrance).Of the present inventionly
The odor type material that remote edge parent material requirement carries Yunnan glutinous rice blood relationship is managed, " four is early synchronous " multi-environment orientation stress Comprehensive Control
The restorer that breeding method is bred as has following characteristics characteristic：Restorer main quality index reaches 3 grades of national standard, savory；Rice blast
Sick better resistance；Whole spice type Hybrid Rice Combination can be prepared, and by authorization, (sterile line, restorer are fragrant rice, and scent gene etc.
Position).Cultivating paddy rice new restorer line using this method has convenient, workable, the advantages of shortening the breeding time limit, extensive being lifted
The index of quality for being again, is that the cultivation of high-grade high quality white rice is done so as to improve the success rate of whole spice type Fine Quality Hybrid Rice seed selection
Go out contribution.
On the basis of the present invention utilizes geographical distant hybridization to hybrid vigour favorable attributes, breeding high-yield amount coordinate force recovers
It is to realize the breakthrough of yield target character；On rice quality, using " high-quality × high-quality " import scent gene and in it is low straight
Chain content of starch gene, and the low chalkness ratio of stress M8003 line, low chalkiness degree and high head rice rate under the conditions of the high temperature dry season；It is anti-
Property degeneration-resistant aspect, resistant gene is imported using " high-quality × disease-resistant ", passes through artificial infection and spontaneous induction and combines screening disease resistance
Strong strain；The degeneration-resistant Sexual behavior mode of high- and low-temperature resistance is carried out using nature high/low temperature；In comprehensive agronomy character, mutually covered using character
Row plant and leaf shape is improved, and vegetative growth phase and biological yield is improved with proper extension breeding time, increase plant height, so as to carry
High economic flow rate；On Spike-grain structure, appropriateness increase grain weight, grain number per spike is taken into account again with grain, and storehouse source stream is coordinated to solve the height of kind
Produce yield stability.
Brief description of the drawings
Fig. 1 is the method flow diagram of integrated cultural hybrid rice odor type restorer provided in an embodiment of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
The method of the present invention is that, using the hybridization of geographical distant race breeding parent material, integrated use " four is early synchronous " multi-environment is fixed
To stress breeding technique and method, cultivate and obtain " two high one excellent one is fragrant " (high-combining ability, high resistant to rice blast, high-quality, fragrance)
Restorer, and it is successfully applied to production.By using geographical distant hybridization to hybrid vigour favorable attributes on the basis of, use
Hybridize to realize quality, yield and resistance between the well-known breeding parent materials such as " high-quality × high-quality ", " high-quality × disease-resistant "
Synchronous improvement, the breeding strategy for the Hybrid Aromatic Rice restorer that breeding goal character is prominent, Comprehensive Traits are excellent.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, the method for integrated cultural hybrid rice odor type restorer provided in an embodiment of the present invention is including following
Step：
S101 is using advantage of the geographical distant hybridization to hybrid, with four early synchronous multi-environment orientation stress selection choosings
Educate high yield coordinate force restorer；
S102：Scent gene and middle low amylose content gene are imported using " high-quality × high-quality ", and in the high temperature dry season
Under the conditions of stress M8003 line low chalkness ratio, low chalkiness degree and high head rice rate；
S103：Resistant gene is imported using " high-quality × disease-resistant ", is combined by artificial infection and spontaneous induction and screens disease-resistant
The strong strain of property；The degeneration-resistant Sexual behavior mode of high- and low-temperature resistance is carried out using nature high/low temperature；
S104：Plant and leaf shape improvement is carried out using character complementation, battalion is improved with proper extension breeding time, increase plant height
Health is long-term and biological yield；
S105：On Spike-grain structure, appropriateness increase grain weight, grain number per spike is taken into account again with grain, and storehouse source stream is coordinated to solve kind
Stable high yield.
The method of integrated cultural hybrid rice odor type restorer provided in an embodiment of the present invention specifically includes following steps：
1st, the excavation of geographical distant race parent material：Yunnan is as one of Asian Cultivated Rice centre of origin, and rice biological is various
Property is higher, is only second to Guangdong, Guangxi.Select material or hybridization intermediate materials with the fragrant glutinous relationship in Yunnan and high-quality, it is disease-resistant excellent
Good backbone parent material hybridization, reestablishes diplomatic relations.
2nd, novelty establishes " four is early synchronous " multi-environment orientation stress selection technique and selection, and integrated use should
Method cultivates hybrid rice odor type restorer.
1. early generation (F2) the screening and assessment system of rice quality is set up：From early generation (F2) in positive season and strange land, field and interior
Seed selection is combined progress early for Quality Identification, significantly improves quality efficiency of selection.
Exterior quality Screening and Identification：In continuous generation more than 3 years, selects fragrance individual plant to use indoors in Sichuan inland river, Lingshui pair, Hainan
Go out rough machine and be ground into brown rice, select complete appearance, no chalk is white or substantially white without chalk, the good brown rice sowing of transparency.In making full use of
The high temperature dry season in the first tenday period of a month river late July 8, orientation stress selection exterior quality and milling quality, it is ensured that comprehensive agronomy character is excellent
It is combined with preferable rice quality.
2. disease-resistant degeneration-resistant early generation (F2) characterization and evaluation technical system is set up
The early generation identification of rice blast resistance：From continuous 3 years of F2 segregating generations selection exterior quality is excellent, dulcet fine individual plant
Carry out many fungus strain mixing artificial infection idenfications of rice blast, the individual plant of orientation stress selection resistance.Selected in annual completion preceding by the end of September
The sowing (inland river greenhouse sick nursery) of material, completes 4 artificial infection idenfications mid-October, and mid or late November completes blast resisting mirror
It is fixed, obtain the consistent material of resistance.
The early generation identification of high- and low-temperature resistance resistance：Make full use of the high temperature dry season in the first tenday period of a month inland river late July 8, orientation stress sieve
Choose roguing system to the endurance of high temperature, quality is discolored for index with colony when the height of setting percentage and maturation.Make full use of rice
Roguing system seedling stage is removed in the natural low temperature orientation stress screening of greenhouse after blast disease resistance identification is complete to the endurance of low temperature, same
One time was identified, using the withered degree size of colony as index.
3. fragrance screening and assessment system, is set up：The tender seedling rubbing method in field, grain yellow maturity grain of rice chew-in technique and laboratory KOH
Solution infusion method identifies fragrance.Cross combination selfing F2 segregating generations continuous 3 generation is protected in seedling stage, the excellent list of maturity period selection from system
Strain carries out fragrance Screening and Identification, and dulcet strain is target strain.
4., set up early for the excellent assessment process of test cross survey：Since making, selection standard extensive combination selfing separates 2 generations, 3 generations is sterile
Manual test cross F1 kinds are done by system, and roguing system hybrid vigour is screened in progress, increase coordinate force seed selection dynamics；Standard sterile line is selected：
Multiple well-known sterile lines such as Zhenshan 97a, II -32A, Gang No.46A, interior fragrant A make Testers, and test cross carries out cooperation force tracking in pairs by hand
Selection, it is ensured that target strain has excellent coordinate force.The apolegamy whole spice type high-quality scented rice combination of reselection odor type sterile line.
3rd, multi-environment orientation stress selective enhancement adaptability
High temperature dry season adverse circumstance：In Breeding Process, we make full use of the high temperature dry season adverse circumstance in inland river rice region to carry out not
Educate is parent and combined fertility, quality, setting percentage, heat resistance Screening and Identification.
Natural low temperature adverse circumstance：Make full use of autumn rice blast be inoculated with after natural low temperature increase breeding material it is low temperature resistant
The selection pressure of characteristic, enhancing sterile line parent material and the adaptability for matching somebody with somebody combination.
Pyricularia oryzae inoculated identification：Carry out many fungus strain mixing artificial infection idenfications of rice blast, orientation stress selection resistance
Strain, screening sterile line parent material and match somebody with somebody combine.
Different ecological environmental screening：Middle roguing is tied up into Sichuan inland river-numerous north in Hainan Lingshui south to educate, breeding of shuttling back and forth,
Select the strain all adapted in far different ecotope.Nan Fanbei, which educates, can be achieved middle roguing system 2 generations of breeding for 1 year, accelerates breeding and enters
Journey, shortens the breeding time limit.
Identification is combined in the room of field：Pass through intuitively comprehensive agronomy character observation, mark, record, the choosing of field breeding material
Select, to laboratory in data analysis, conclude, collect, required excellent target character and excellent genes are polymerize in one, screening
Obtain the target strain for entering next season.
The application principle of the present invention is further described with reference to specific embodiment.
Embodiment 1
Perfume extensive 2156 in the disease-resistant odor type restorer of high-quality high-combining ability
Winter in 1999, with extensive (the rear life of 99 winter 47 in the High quality and diseases resistance odor type restorer in the fragrant glutinous relationship Yunnan auspicious 306 containing Yunnan
Fragrant extensive No. 1, relationship in name：No. 1/narrow leaf of iron plum green grass or young crops //IR28/ //IR24/IR214/ ///Yunnan auspicious 306/ // // bright extensive 63) work is female
This, paternal hybrid is made with extensive 99-14 in self-fertile High quality and diseases resistance restorer, in pedigree Breeding Process, while strengthening to strain rice
The selection of meter Pin Zhi, fragrance, disease resistance and resistance, restoring force and coordinate force, multi-environment increases orientation stress selection pressure, control
System variation direction, polymerize excellent genes.Selection standard test cross sterile line and the continuous test cross of II -32A, Zhenshan 97a, F in 20036Obtain
Shaping strain must be stablized, plant, rice are savory, name as interior perfume extensive 2156.Selection interior perfume 1A, 2A, 3A, 5A are measured, small system
Kind.In early August, 2005 passes through Sichuan Province's technical appraisement.Perfume 8156 is (fragrant extensive in interior fragrant 5A/ in whole spice type High quality and diseases resistance Combination nova
2156) authorized (rice 2006004 is examined in river) by Sichuan within 2006, pass through country's authorization within 2008 (state examines rice 2008002).Specifically
Breeding Process is as shown in table 1.
Fragrant extensive 2156 seed selection flow table in table 1
Extensive 3399 in the disease-resistant odor type restorer of the high-quality high-combining ability of embodiment 2
Winter in 2002 is (extensive containing interior perfume with restorer R789 in the middle of self-fertile High quality and diseases resistance odor type in Hainan Lingshui Nan Fan bases
No. 2 blood relationships：No. 1/narrow leaf of iron plum green grass or young crops //IR28/ //IR24/IR214/ ///Yunnan auspicious 306/ // // it is bright it is extensive 63) with restorer R456
Hybridization, plants F1 in positive season in 2003 in inland river, then is reestablished diplomatic relations with the excellent restorer of comprehensive agronomy character wide extensive 128, the winter in 2003
It is numerous to plant F1 generation of reestablishing diplomatic relations in Hainan Lingshui base.Educated by Nan Fanbei, combining blast resisting CHARACTERISTICS IDENTIFICATION, hybrid vigour by generation sieves
Select identification, Identification of Rice-quality, fragrance identification.In Breeding Process, selection fine individual plant and Zhenshan 97a, II -32A, interior fragrant 5A
Deng sterile line test cross, positive season restoring force in 2005 is strong, coordinate force is high, rice is of fine quality, and economical character is excellent, there is being stable into for fragrant
Type strain 3399 is selected in, and names as interior extensive 3399.In August, 2008, by Sichuan Province's technical appraisement, is F10 generations.Whole spice type high-quality
5 excellent 5399 (extensive 3399) pass through country's authorization (state examines rice 2009005), rice product in 2009 in interior fragrant 5A/ in disease-resistant Combination nova
Matter reaches 2 grades of national standard.Specific Breeding Process is as shown in table 2.
Extensive 3399 seed selection flow table in table 2
Perfume extensive 3306 in the disease-resistant odor type restorer of the high-quality high-combining ability of embodiment 3
Positive season in 2004 inland river using self-fertile High quality and diseases resistance restorer intermediate materials R2565/R5125 (interior extensive No. 2 of perfume //
Extensive 99-14/ //R456 in 99 winters 47/) hybridize with extensive 99-14 in high resistant to rice blast restorer, winter in 2004 is in Hainan Lingshui
Nan Fan bases add generation plantation F1, in plantation F2 generations positive season in 2005, are educated by Nan Fanbei, by generation combination blast resistance identification, miscellaneous
The identification of the advantage of kind, Identification of Rice-quality and fragrance identification.It is selection fine individual plant and II -32A, Gang No.46A, interior in Breeding Process
The sterile line test crosses such as fragrant 5A, interior fragrant 7A, the strain of winter 3306 in 2006 is because restoring force is strong, coordinate force is high, rice is of fine quality, better resistance, have
Fragrance, economical character are excellent selected.The experts field technology identification organized by Sichuan seed station for 2011 and quality
Examine, name as interior perfume extensive 3306, be F14 generations.(fragrant extensive 3306) in interior fragrant 5A/ 5 excellent 306 in whole spice type High quality and diseases resistance Combination nova
Authorized (rice 2012001 is examined in river) by Sichuan within 2012, rice quality reaches 3 grades of national standard.Specific Breeding Process is as shown in table 3.
Fragrant extensive 3306 seed selection flow table in table 3
The hybrid rice odor type restorer feature that the embodiment of the present invention 1,2 and 3 is cultivated is as follows：
1st, comprehensive agronomy character is excellent
Extensive 2156 grade of interior perfume 3 is investigated in inland river special for the form of 14 such as strain, fringe, leaf, breeding time of examination restorer
Levy and show and (be shown in Table 4), the comprehensive agronomy character of interior fragrant sterile line is excellent, there is longer nutrition breeding time, to improve biology production
Amount and hybrid vigour yield are laid a good foundation.
Perfume extensive 2156, interior extensive 3399, the feature of extensive 3306 major traits of interior perfume in table 4
2nd, itself exterior quality is excellent
Through being carried out continuously rice outward appearance, processing, Cooking Quality screening：Interior fragrant restorer integrated quality excellent, low chalk
White and high head rice rate is protruded, hence it is evident that use Mianhui725 (CK) better than the same period.It is mainly manifested in and contains with middle low amylose
Amount, and the important quality indicator such as chalkness ratio, chalkiness degree, gel consistence and head rice rate entirely reaches state and issues 3 grades of standards of high quality paddy,
It is one of best odor type restorer that the quality traits such as outward appearance, processing, boiling, food flavor are combined.
The rice quality of fragrant restorer in table 5
3rd, interior fragrant restorer high-quality hereditary capacity is strong
The combination rice quality leading indicator that interior fragrant restorer is prepared reaches national standard more than 3 grades, wherein interior 5 excellent 5399 rice product
Matter index reaches 2 grades of national standard, and is the combination of whole spice type grain quality rice, and stem, leaf, flower and every rice are savory.
Table 6 is by authorizing the whole spice type fine quality up to more than 3 grades of national standard
4th, rice blast resistance is stronger
Using the throughout the year popular rice blast fungus strain in Sichuan, internally fragrant restorer is inoculated with field test result (table 7) with compareing,
1~3 grade of the restorer leaf pest, 1~3 grade of neck pest such as wherein interior perfume extensive 2156, hence it is evident that better than control Mianhui725.
Fragrant restorer blast resistance identification rating result unit in table 7：Level
Interior fragrant restorer blast resisting genetic force is strong, matches somebody with somebody that kind rice blast is tried in national area, disease resistance is tried in Sichuan provinces and regions
Prominent (table 8).
The disease-resistant resistance of fragrant kind in 83 whole spices of table
5th, cold resistance is stronger
The internal fragrant restorer of natural low temperature stress after the completion of being identified using annual autumn rice blast leaf pest is with compareing progress
Cold tolerance at the seedling stage field test shows (table 9) that interior fragrant restorer is stronger than the Mianhui725 that the same period uses to the cold resistance of low temperature, its
In in the fragrant restorer cold tolerance at the seedling stage such as extensive 2156 be 5~7 grades, field is resisted cold, and relatively to compare Mianhui725 strong for sex expression.
Fragrant sterile line and conventional sterile line cold resistance in table 9
6th, yield traits general combining ability is high
9 × 6 coordinate force diallel crosses experiment in 2012 shows that interior perfume extensive 3306 (R3) is in effective fringe, plant height, total grain panicle
Number, Spikelet density, show as positive-effect on duration from seeding to heading.
Fragrant extensive 3306 (R3) parent's Other Main Agronomic Characters general combining ability (GCA) effect in table 9
Value
Those skilled in the art of the present technique are appreciated that unless otherwise defined, all terms used herein (including technology art
Language and scientific terminology) with the general understanding identical meaning with the those of ordinary skill in art of the present invention.Should also
Understand, those terms defined in such as general dictionary, which should be understood that, to be had and the meaning in the context of prior art
The consistent meaning of justice, and unless defined as here, will not be explained with idealization or excessively formal implication.
It should be noted last that：Above example is only used to illustrative and not limiting technical scheme, although ginseng
The present invention is described in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that：Still can be to this
Invention is modified or equivalent substitution, any modification or partial replacement without departing from the spirit and scope of the present invention, and its is equal
It should cover among scope of the presently claimed invention.
Claims (8)
1. a kind of method of integrated cultural hybrid rice odor type restorer, it is characterised in that step includes：
S101 is high with four early synchronous multi-environment orientation stress selection seed selections using advantage of the geographical distant hybridization to hybrid
Yield combining ability restorer；
S102：Scent gene and middle low amylose content gene are imported using " high-quality × high-quality ", and in high temperature dry season condition
The low chalkness ratio of lower stress M8003 line, low chalkiness degree and high head rice rate；
S103：Resistant gene is imported using " high-quality × disease-resistant ", it is strong to combine screening disease resistance by artificial infection and spontaneous induction
Strain；The degeneration-resistant Sexual behavior mode of high- and low-temperature resistance is carried out using nature high/low temperature；
S104：Plant and leaf shape improvement is carried out using character complementation, nutrition life is improved with proper extension breeding time, increase plant height
Long-term and biological yield；
S105：On Spike-grain structure, appropriateness increase grain weight, grain number per spike is taken into account again with grain, and storehouse source stream is coordinated to solve the high yield of kind
Yield stability.
2. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 1, it is characterised in that the high yield is matched somebody with somebody
It is material of the selection with the fragrant glutinous relationship in Yunnan that restorer, which is cultivated, with joint efforts or it hybridizes intermediate materials and excellent backbone parent material is miscellaneous
Hand over, reestablish diplomatic relations.
3. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 1, it is characterised in that described in S101
Four early synchronous multi-environment orientations coerce selection steps and are：
1. rice quality is set up early for screening and assessment system：From early Dai Zheng seasons with strange land, field with indoor seed selection be combined into
Row is early for Quality Identification；
2. disease-resistant degeneration-resistant morning is set up for characterization and evaluation；
3. fragrance is set up early for characterization and evaluation；
4. set up early for the excellent evaluation of test cross survey：Selection standard sterile line does manual test cross F1 kinds since the generation of selfing 2,3 generations, carries out
The hybrid vigour screening of middle roguing system, increases coordinate force seed selection dynamics；Standard sterile line is selected：Zhenshan 97a, II -32A, Gang No.46A,
Multiple well-known sterile lines such as interior fragrant A make Testers.
4. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 3, it is characterised in that 1. step, builds
Vertical rice quality early includes for screening and assessment system：
Exterior quality Screening and Identification：In continuous generation more than 3 years, selects fragrance individual plant rough with going out indoors in Sichuan inland river, Lingshui pair, Hainan
Machine is ground into brown rice, selects complete appearance, and no chalk is white or substantially white without chalk, the good brown rice sowing of transparency.
5. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 3, it is characterised in that 2. step, is set up
Disease-resistant degeneration-resistant morning includes for characterization and evaluation：
The early generation identification of rice blast resistance, from morning for continuous 3 years selection exterior qualities of segregating generation are excellent, dulcet fine individual plant enters
The many fungus strain mixing artificial infection idenfications of row rice blast, the individual plant of orientation stress selection resistance；Selection in annual completion preceding by the end of September
The sowing of material, completes 4 artificial infection idenfications mid-October, and mid or late November completes blast resisting identification, obtains resistance consistent
Material；
The early generation identification of high- and low-temperature resistance resistance, using the high temperature dry season in the first tenday period of a month inland river late July 8, roguing in orientation stress screening
It is the endurance to high temperature, quality is discolored for index with colony when the height of setting percentage and maturation；Utilize blast resistance identification
Remove roguing system seedling stage in the natural low temperature orientation stress screening of greenhouse after complete, to the endurance of low temperature, identifies in the same time,
Using the withered degree size of colony as index.
6. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 3, it is characterised in that 3. step, is set up
Fragrance is early for characterization and evaluation, including：The tender seedling rubbing method in field, grain yellow maturity grain of rice chew-in technique and laboratory KOH solution infusion method
Identify fragrance；Enter to hold or participate in a prayer service at a temple in seedling stage, maturity period selection fine individual plant from extensive cross combination early selfing line segregating generation continuous 3 generation is made
Taste Screening and Identification, dulcet strain is target strain.
7. the method for integrated cultural hybrid rice odor type restorer as claimed in claim 3, it is characterised in that 4. step, is set up
It is early to survey excellent assessment process for test cross：Manual test cross F1 is since making selection standard sterile line extensive combination selfing separates 2 generations, 3 generations
Kind, roguing system hybrid vigour is screened in progress, increases coordinate force seed selection dynamics；Reselection odor type sterile line matches whole spice type high-quality
Rice combines.
8. the method for integrated cultural hybrid rice odor type restorer as described in claim 3 or 6, it is characterised in that the standard
One or more during sterile line selection is following：
Zhenshan 97a, II -32A, Gang No.46A, interior fragrant A.
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| CN108849482A (en) * | 2018-08-01 | 2018-11-23 | 重庆市农业科学院 | A kind of selection of hybrid rice restorer line |
| CN108967185A (en) * | 2018-09-04 | 2018-12-11 | 江苏里下河地区农业科学研究所 | The selection of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting |
| CN109122438A (en) * | 2018-09-06 | 2019-01-04 | 中国科学院烟台海岸带研究所 | A kind of purple sea hybrid scallop bottom sowing aquaculture method |
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN111771717A (en) * | 2020-07-28 | 2020-10-16 | 怀化职业技术学院 | Method for comprehensively cultivating hybrid rice scent type restorer line |
| CN115191342A (en) * | 2022-07-14 | 2022-10-18 | 四川省农业科学院水稻高粱研究所 | Breeding method of high-temperature-resistant drought-resistant high-quality rice restorer line |
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| CN104823843A (en) * | 2015-05-06 | 2015-08-12 | 云南种业集团有限责任公司 | Breeding method of one-restore dual-purpose aromatic soft hybrid rice restorer line |
| CN106508663A (en) * | 2016-10-26 | 2017-03-22 | 内江杂交水稻科技开发中心 | Method for comprehensive cultivation of sterile line of three-line hybrid rice |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104823843A (en) * | 2015-05-06 | 2015-08-12 | 云南种业集团有限责任公司 | Breeding method of one-restore dual-purpose aromatic soft hybrid rice restorer line |
| CN106508663A (en) * | 2016-10-26 | 2017-03-22 | 内江杂交水稻科技开发中心 | Method for comprehensive cultivation of sterile line of three-line hybrid rice |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849482A (en) * | 2018-08-01 | 2018-11-23 | 重庆市农业科学院 | A kind of selection of hybrid rice restorer line |
| CN108967185A (en) * | 2018-09-04 | 2018-12-11 | 江苏里下河地区农业科学研究所 | The selection of the high-quality strong advantage Indica Rice Restorer Lines of blast resisting |
| CN108967185B (en) * | 2018-09-04 | 2021-09-10 | 江苏里下河地区农业科学研究所 | Breeding method of high-quality rice blast resistance strong-advantage indica rice restorer line |
| CN109122438A (en) * | 2018-09-06 | 2019-01-04 | 中国科学院烟台海岸带研究所 | A kind of purple sea hybrid scallop bottom sowing aquaculture method |
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN111771717A (en) * | 2020-07-28 | 2020-10-16 | 怀化职业技术学院 | Method for comprehensively cultivating hybrid rice scent type restorer line |
| CN115191342A (en) * | 2022-07-14 | 2022-10-18 | 四川省农业科学院水稻高粱研究所 | Breeding method of high-temperature-resistant drought-resistant high-quality rice restorer line |
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Effective date of registration: 20190325 Address after: No. 401 Huaitan Road, Central District, Neijiang City, Sichuan Province, 641000 Applicant after: Hybrid rice Science and Technology Development Center, inland river Applicant after: China National Rice Research Institute Applicant after: SICHUAN PROVINCE NEIJIANG CITY ACADEMY OF AGRICULTURAL SCIENCE Address before: 641000 No. 401 Huaitan Road, Shizhong District, Neijiang City, Sichuan Province Applicant before: Hybrid rice Science and Technology Development Center, inland river |
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Application publication date: 20170721 |