CN109757360A - A kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties - Google Patents
A kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties Download PDFInfo
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Abstract
The present invention relates to rice breeding technology field, especially a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties includes the following steps；A, the cultivation of the black perfume 1A of the high-quality black rice sterile line of odor type；B, the cultivation of the high-quality round-grained rice restorer perfume extensive 78 of odor type；C, the cultivation of the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties.The present invention, which has, selects high-quality, better resistance kind to make parent, improves that filial generation quality is excellent, frequency of resistance, the characteristics of taking correctly hybridization and complex variable method, effectively improve the junction frequency of beneficial traits.
Description
Technical field
The present invention relates to rice breeding technology field more particularly to a kind of whole spice type good-quality high-yield hybrid black rice new varieties
Breeding method.
Background technique
Black rice is that kind of skin and aleurone contain special natural pitch black color substance -- it is " rice melanin " (i.e. anthocyanin), rough
Rice is in a kind of rice of black.It is documented that Black Rice is the ancient and rare seed rice in China, the rice made with black rice,
Integrate color, nutrition and health care, it is civil to be referred to as " rice of enriching blood ", " medicine rice ", " long-lived rice ", there is " king in rice "
Laudatory title.Show black rice because keeping the nutrition and health care value ratio rice of black rice higher rich in atrament according to modern study data.
Black rice is not the staple food rice of our people, and black rice adds primarily as food ingredient, custom articles and non-staple food
The raw material of work, the consumption figure that black rice directly works as staple food is few, therefore black rice Advances in Breeding is slow more than common rice rice Advances in Breeding.
The black rice varieties quantity brought out is few, and the old kind in some tradition places mainly continued to use in production, is mostly that service life is more long
Conventional black rice, kind sexual involution, low output, productivity effect are poor.And high yield, high-quality, stable yields, the extensively excellent hybridization of Comprehensive Traits such as suitable
Black rice is in great shortage, and especially there is not been reported so far for the hybridization black rice of high yield and high quality whole spice type.Because of the lag of black rice breeding work, sternly
The development of black rice production is constrained again, is not able to satisfy urgent need of the consumer to high-quality black rice.In view of the above-mentioned problems, in recent years
Come, Shannon industry Science and Technology Co., Ltd. and Xiaogan City Xiaonan District Institute of agricultural sciences research cooperation in Hubei, development rise
The breeding of whole spice type high yield and high quality hybridization black rice new varieties.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of whole spice type good quality and high output proposed
Hybridize the breeding method of black rice new varieties.
To achieve the goals above, present invention employs following technical solutions:
Design a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties, comprising the following steps:
A, the cultivation of the black perfume 1A of the high-quality black rice sterile line of odor type:
A1, incubation experiment: three line method is used, the black sterile line of BT type nucleo-cytoplasmic interreaction japonica rice is cultivated；
A2, Juvenile stage: the parental source of black perfume 1A are as follows: [force fortune round-grained rice 7A/ (the black round-grained rice of 17//Hubei Province of evening, Hubei Province, 17/ Xiaogan of evening)]；
A3, Crossing system: make female parent with Hubei Province evening 17 first, make male parent single cross with the black round-grained rice of Xiaogan, F1 is obtained, then with above-mentioned
F1 makees male parent, makees circulation female parent with Hubei Province evening 17, is returned, obtains F2, in backcrossing F2 group, selects multiple purple leaf black rice fragrance
Homozygous elite plant makees male parent, makees paired cross with force fortune round-grained rice 7A and surveys guarantor, protects pairing to the survey that sterility has been kept and continues to connect in pairs
Continuous backcrossing, until cultivating the stable black perfume 1A of odor type purple leaf black rice sterile line and keeping system；
Black perfume 1A: E Wan 17 of a4, cultivation odor type purple leaf black rice sterile line makees female parent, makees paternal hybrid with the black round-grained rice of Xiaogan, obtains
Hybrid seed is obtained, F1 is then planted, then make female parent with Hubei Province evening 17, makees male parent backcrossing with Hybrids F1, obtain backcrossing seed, then plant
Backcrossing F1 is planted, when mature, is identified in conjunction with fragrance, roguing shape is partially female, fragrance is homozygous, brown rice aterrimus fine individual plant, then plants
It is returned F2, greenery seedling is eliminated, retains purple Ye Miao, does paired cross when heading with No. 7 A of Wu Yun round-grained rice, obtains to survey and protects female parent seed,
Then it is sub (i.e. pairing backcrossing F1) to survey conservation for plantation, while corresponding plantation surveys and protects male parent, when heading, in conjunction with pollen microscopic examination, observation
It surveys and protects maternal strain sterility and separation situation, sterility is kept as, and the pairing system continuation that male parent economical character is excellent
Pairing backcrossing, hereafter, with ibid method, continuous pairs backcrossing when being returned F9, has cultivated sterility stabilization, economical character
Neat odor type purple leaf black rice sterile line black perfume 1A and black perfume 1B；
B, the cultivation of the high-quality round-grained rice restorer perfume extensive 78 of odor type:
B1, Juvenile stage: the parental source of male parent perfume extensive 78 is [R254/ //C418//C418/ perfume (or spice) round-grained rice 9505]；
B2, Crossing system: make female parent with Elite restorer line C418 first, make paternal hybrid with fragrant round-grained rice 9505, hybridized
Then seed makees male parent with above-mentioned Hybrids F1, make circulation female parent with C418, be returned, and make male parent with backcrossing F1, use is excellent
Restorer R254 makees maternal, reestablishes diplomatic relations, in the offspring that reestablishes diplomatic relations, in conjunction with the methods of fragrance detection, restorability test, and directive breeding,
Target restorer perfume extensive 78 is brought out；
B3, the process for cultivating restorer perfume extensive 78: round-grained rice restorer C418 makees maternal in use, and it is miscellaneous to make male parent with fragrant round-grained rice 9505
It hands over, makees male parent with above-mentioned Hybrids F1, and make circulation mother backcrossing with C418, then plantation backcrossing F1, it is extensive in heading and excellent late round-grained rice
Again it is that R254 reestablishes diplomatic relations, obtains seed of reestablishing diplomatic relations, then plant the F1 that reestablishes diplomatic relations, detected through fragrance, the elected dulcet fine individual plant of tool stays
Kind, then reestablish diplomatic relations F2, this generation, chooses elite plant strain, and fragrance is combined to detect, and every is that elected fragrance is homozygous and Comprehensive Traits are excellent
Good single plant is reserved seed for planting, and then reestablish diplomatic relations F3, this generation, and in heading, every system selects elite plant, and be listed singling, transports round-grained rice 7A with sterile line force,
It is made to restorability test, harvest surveys extensive male parent and corresponding test cross seed, then plants the F4 that reestablishes diplomatic relations, and plant corresponding test cross
F1, in the restoring degree of primary part observation test cross F1, hybrid vigour and the economical character for corresponding to male parent, this generation, are elected to restoring degree after heading
Height, hybrid vigour is strong, economical character is excellent, the small male parent strain of separating degree, chooses elite plant, and be listed singling, transports with sterile line force
Round-grained rice 7A, repetition are made to restorability test, and harvest surveys extensive male parent and hereafter corresponding test cross seed with ibid method, that is, is reestablished diplomatic relations
F14 has cultivated the japonica rice that restoring degree is good, coordinate force is high, quality is excellent, fragrance is homozygous, Comprehensive Traits are excellent, economical character is neat
Restorer perfume extensive 78；
C, the cultivation of the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties:
C1, make female parent with the black perfume 1A of obtained odor type purple leaf black rice round-grained rice sterile line, made with self-fertile odor type restorer fragrant extensive 78
Male parent obtains the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties through hybridization；
Preferably, in step a3, traditional black japonica rice of local varieties Xiaogan is selected to make male parent.
Preferably, maternal is black rice, and cauline leaf is in aubergine；Male parent is rice, and cauline leaf is in green；Parent hybridization, obtains
Hybrids F1 rice be aterrimus, and cauline leaf be green.
Preferably, Parent is in the cultivating process of every generation, requires to carry out fragrance identification, to fragrance carry out tracking and
Selection guarantees that fragrance is not lost.
Preferably, when F1 and excellent evening round-grained rice restorer R254 reestablish diplomatic relations, with molecular marker assisted selection method, choosing carries perfume (or spice)
The elite plant of taste gene is reestablished diplomatic relations F1.
Preferably, step A and step B is carried out simultaneously, the black excellent training of perfume of whole spice type high-quality hybrid black rice new varieties can be shortened
The time brought out.
A kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties proposed by the present invention, beneficial effect are:
It selects high-quality, better resistance kind to make parent, improves that filial generation quality is excellent, frequency of resistance, take correct hybridization
With complex variable method, the rice for effectively improving the junction frequency of beneficial traits, while cultivating, brown rice aterrimus, after processing
The rice that rice with part Black seed coat is made, color is scarlet, and aromatic flavour is pure, glossy soft, sweet and dilitious, food flavor
Fabulous, single food, with eating, cooking, cooking congee, yield is high, and yielding ability is good, and stalk is more sturdy, and toughness is good, fertilizer resistance anti-lodging
By force.
Specific embodiment
Below in conjunction in the embodiment of the present invention, technical solution in the embodiment of the present invention is clearly and completely retouched
It states, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
A kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties, comprising the following steps:
A, the cultivation of the black perfume 1A of the high-quality black rice sterile line of odor type:
A1, incubation experiment: three line method is used, the black sterile line of BT type nucleo-cytoplasmic interreaction japonica rice is cultivated；
A2, Juvenile stage: the parental source of black perfume 1A are as follows: [force fortune round-grained rice 7A/ (the black round-grained rice of 17//Hubei Province of evening, Hubei Province, 17/ Xiaogan of evening)]；
A3, Crossing system: make female parent with Hubei Province evening 17 first, make male parent single cross with the black round-grained rice of Xiaogan, F1 is obtained, then with above-mentioned
F1 makees male parent, makees circulation female parent with Hubei Province evening 17, is returned, obtains F2, in backcrossing F2 group, selects multiple purple leaf black rice fragrance
Homozygous elite plant makees male parent, makees paired cross with force fortune round-grained rice 7A and surveys guarantor, protects pairing to the survey that sterility has been kept and continues to connect in pairs
Continuous backcrossing, until cultivating the stable black perfume 1A of odor type purple leaf black rice sterile line and keeping system；
Black perfume 1A: E Wan 17 of a4, cultivation odor type purple leaf black rice sterile line makees female parent, makees paternal hybrid with the black round-grained rice of Xiaogan, obtains
Hybrid seed is obtained, F1 is then planted, then make female parent with Hubei Province evening 17, makees male parent backcrossing with Hybrids F1, obtain backcrossing seed, then plant
Backcrossing F1 is planted, when mature, is identified in conjunction with fragrance, roguing shape is partially female, fragrance is homozygous, brown rice aterrimus fine individual plant, then plants
It is returned F2, greenery seedling is eliminated, retains purple Ye Miao, does paired cross when heading with No. 7 A of Wu Yun round-grained rice, obtains to survey and protects female parent seed,
Then it is sub (i.e. pairing backcrossing F1) to survey conservation for plantation, while corresponding plantation surveys and protects male parent, when heading, in conjunction with pollen microscopic examination, observation
It surveys and protects maternal strain sterility and separation situation, sterility is kept as, and the pairing system continuation that male parent economical character is excellent
Pairing backcrossing, hereafter, with ibid method, continuous pairs backcrossing when being returned F9, has cultivated sterility stabilization, economical character
Neat odor type purple leaf black rice sterile line black perfume 1A and black perfume 1B；
B, the cultivation of the high-quality round-grained rice restorer perfume extensive 78 of odor type:
B1, Juvenile stage: the parental source of male parent perfume extensive 78 is [R254/ //C418//C418/ perfume (or spice) round-grained rice 9505]；
B2, Crossing system: make female parent with Elite restorer line C418 first, make paternal hybrid with fragrant round-grained rice 9505, hybridized
Then seed makees male parent with above-mentioned Hybrids F1, make circulation female parent with C418, be returned, and make male parent with backcrossing F1, use is excellent
Restorer R254 makees maternal, reestablishes diplomatic relations, in the offspring that reestablishes diplomatic relations, in conjunction with the methods of fragrance detection, restorability test, and directive breeding,
Target restorer perfume extensive 78 is brought out；
B3, the process for cultivating restorer perfume extensive 78: round-grained rice restorer C418 makees maternal in use, and it is miscellaneous to make male parent with fragrant round-grained rice 9505
It hands over, makees male parent with above-mentioned Hybrids F1, and make circulation mother backcrossing with C418, then plantation backcrossing F1, it is extensive in heading and excellent late round-grained rice
Again it is that R254 reestablishes diplomatic relations, obtains seed of reestablishing diplomatic relations, then plant the F1 that reestablishes diplomatic relations, detected through fragrance, the elected dulcet fine individual plant of tool stays
Kind, then reestablish diplomatic relations F2, this generation, chooses elite plant strain, and fragrance is combined to detect, and every is that elected fragrance is homozygous and Comprehensive Traits are excellent
Good single plant is reserved seed for planting, and then reestablish diplomatic relations F3, this generation, and in heading, every system selects elite plant, and be listed singling, transports round-grained rice 7A with sterile line force,
It is made to restorability test, harvest surveys extensive male parent and corresponding test cross seed, then plants the F4 that reestablishes diplomatic relations, and plant corresponding test cross
F1, in the restoring degree of primary part observation test cross F1, hybrid vigour and the economical character for corresponding to male parent, this generation, are elected to restoring degree after heading
Height, hybrid vigour is strong, economical character is excellent, the small male parent strain of separating degree, chooses elite plant, and be listed singling, transports with sterile line force
Round-grained rice 7A, repetition are made to restorability test, and harvest surveys extensive male parent and hereafter corresponding test cross seed with ibid method, that is, is reestablished diplomatic relations
F14 has cultivated the japonica rice that restoring degree is good, coordinate force is high, quality is excellent, fragrance is homozygous, Comprehensive Traits are excellent, economical character is neat
Restorer perfume extensive 78；
C, the cultivation of the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties:
C1, make female parent with the black perfume 1A of obtained odor type purple leaf black rice round-grained rice sterile line, made with self-fertile odor type restorer fragrant extensive 78
Male parent obtains the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties through hybridization；
In step a3, traditional black japonica rice of local varieties Xiaogan is selected to make male parent.
Female parent is black rice, and cauline leaf is in aubergine；Male parent is rice, and cauline leaf is in green；Parent hybridization, the Hybrids F1 of acquisition
Rice is aterrimus, and cauline leaf is green.
Parent requires to carry out fragrance identification, is tracked and selected to fragrance, protect in the cultivating process of every generation
Card fragrance is not lost.
When F1 and excellent evening round-grained rice restorer R254 reestablish diplomatic relations, with molecular marker assisted selection method, choosing carries scent gene
Elite plant reestablish diplomatic relations F1.
Step A and step B is carried out simultaneously, can shorten the black perfume of whole spice type high-quality hybrid black rice new varieties it is excellent cultivate when
Between.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties, which comprises the following steps:
A, the cultivation of the black perfume 1A of the high-quality black rice sterile line of odor type:
A1, incubation experiment: three line method is used, the black sterile line of BT type nucleo-cytoplasmic interreaction japonica rice is cultivated；
A2, Juvenile stage: the parental source of black perfume 1A are as follows: [force fortune round-grained rice 7A/ (the black round-grained rice of 17//Hubei Province of evening, Hubei Province, 17/ Xiaogan of evening)]；
A3, Crossing system: make female parent with Hubei Province evening 17 first, make male parent single cross with the black round-grained rice of Xiaogan, obtain F1, then made with above-mentioned F1
Male parent makees circulation female parent with Hubei Province evening 17, is returned, obtains F2, in backcrossing F2 group, selects multiple purple leaf black rice fragrance homozygous
Elite plant makees male parent, makees paired cross with force fortune round-grained rice 7A and surveys guarantor, protects pairing to the survey that sterility has been kept and continues continuously to return in pairs
It hands over, until cultivating the stable black perfume 1A of odor type purple leaf black rice sterile line and keeping system；
Black perfume 1A: E Wan 17 of a4, cultivation odor type purple leaf black rice sterile line makees female parent, makees paternal hybrid with the black round-grained rice of Xiaogan, obtains miscellaneous
Seed is handed over, F1 is then planted, then make female parent with Hubei Province evening 17, makees male parent backcrossing with Hybrids F1, obtain backcrossing seed, then plant back
F1 is handed over, when mature, is identified in conjunction with fragrance, roguing shape is partially female, fragrance is homozygous, brown rice aterrimus fine individual plant, then plantation backcrossing
F2 eliminates greenery seedling, retains purple Ye Miao, does paired cross when heading with No. 7 A of Wu Yun round-grained rice, obtains to survey and protects female parent seed, then
It is sub (i.e. pairing backcrossing F1) that conservation is surveyed in plantation, while corresponding plantation surveys and protects male parent, and when heading, in conjunction with pollen microscopic examination, observation, which is surveyed, to be protected
Maternal strain sterility and separation situation, are kept as sterility, and the excellent pairing system of male parent economical character continue it is pairs of
Backcrossing, hereafter, with ibid method, when being returned F9, it is neat to have cultivated sterility stabilization, economical character for continuous pairs backcrossing
Odor type purple leaf black rice sterile line black perfume 1A and black perfume 1B；
B, the cultivation of the high-quality round-grained rice restorer perfume extensive 78 of odor type:
B1, Juvenile stage: the parental source of male parent perfume extensive 78 is [R254/ //C418//C418/ perfume (or spice) round-grained rice 9505]；
B2, Crossing system: making female parent with Elite restorer line C418 first, makees paternal hybrid with fragrant round-grained rice 9505, obtains hybrid seed,
Then make male parent with above-mentioned Hybrids F1, make circulation female parent with C418, be returned, and make male parent with backcrossing F1, with excellent recovery
It is that R254 makees female parent, reestablishes diplomatic relations, in the offspring that reestablishes diplomatic relations, in conjunction with the methods of fragrance detection, restorability test, directive breeding is brought out
Target restorer perfume extensive 78；
B3, the process for cultivating restorer perfume extensive 78: round-grained rice restorer C418 makees maternal in use, makees paternal hybrid with fragrant round-grained rice 9505, uses
Above-mentioned Hybrids F1 makees male parent, and makees circulation mother backcrossing with C418, then plantation backcrossing F1, in heading and excellent late round-grained rice restorer
R254 reestablishes diplomatic relations, and obtains seed of reestablishing diplomatic relations, and then plants the F1 that reestablishes diplomatic relations, detects through fragrance, and the elected dulcet fine individual plant of tool is reserved seed for planting, so
After reestablish diplomatic relations F2, in this generation, chooses elite plant strain, and fragrance is combined to detect, and every is the list that elected fragrance is homozygous and Comprehensive Traits are excellent
Strain is reserved seed for planting, and then reestablish diplomatic relations F3, this generation, and in heading, every system selects elite plant, and be listed singling, is transported round-grained rice 7A with sterile line force, is made pair
Restorability test, harvest survey extensive male parent and corresponding test cross seed, then plant the F4 that reestablishes diplomatic relations, and plant corresponding test cross F1, are taking out
In the restoring degree of primary part observation test cross F1, hybrid vigour and the economical character for corresponding to male parent after fringe, this generation, are elected to restoring degree height, hybrid
Advantage is strong, economical character is excellent, the small male parent strain of separating degree, chooses elite plant, and be listed singling, transports round-grained rice 7A, weight with sterile line force
It is made again to restorability test, harvest surveys extensive male parent and corresponding test cross seed, and hereafter, with ibid method, that is, reestablish diplomatic relations F14, cultivates
It is fragrant the Restoring Line of Japonica Rice that restoring degree is good, coordinate force is high, quality is excellent, fragrance is homozygous, Comprehensive Traits are excellent, economical character is neat has been gone out
Extensive 78；
C, the cultivation of the black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties:
C1, make female parent with the black perfume 1A of obtained odor type purple leaf black rice round-grained rice sterile line, make male parent with self-fertile odor type restorer perfume extensive 78,
The black perfume excellent 178 of whole spice type high-quality hybrid black rice new varieties is obtained through hybridization.
2. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties according to claim 1, feature exist
In in step a3, the traditional black japonica rice of local varieties Xiaogan of selection makees male parent.
3. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties according to claim 1, feature exist
In female parent is black rice, and cauline leaf is in aubergine；Male parent is rice, and cauline leaf is in green；Parent hybridization, the Hybrids F1 rice of acquisition
For aterrimus, and cauline leaf is green.
4. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties according to claim 1, feature exist
In Parent requires to carry out fragrance identification, tracked and selected to fragrance in the cultivating process of every generation, guarantees fragrant
Taste is not lost.
5. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties according to claim 1, feature exist
In when F1 and excellent evening round-grained rice restorer R254 reestablish diplomatic relations, with molecular marker assisted selection method, choosing carries the excellent of scent gene
Strain is reestablished diplomatic relations F1.
6. a kind of breeding method of whole spice type good-quality high-yield hybrid black rice new varieties according to claim 1, feature exist
In being carried out simultaneously to step A and step B, the whole spice type high-quality hybrid black rice new varieties black perfume excellent time cultivated can be shortened.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111328699A (en) * | 2020-01-21 | 2020-06-26 | 江苏沿海地区农业科学研究所 | Breeding method of rice variety with purple black yellow glume seed coats |
| CN111631129A (en) * | 2020-05-26 | 2020-09-08 | 上海市农业科学院 | Rapid breeding method of fragrant and soft hybrid japonica rice sterile line |
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| CN104170724A (en) * | 2014-08-14 | 2014-12-03 | 淮南市开元生物技术研究中心 | Breeding method for aromatic hybrid rice Guangxiangyou 36 |
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
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| CN104170724A (en) * | 2014-08-14 | 2014-12-03 | 淮南市开元生物技术研究中心 | Breeding method for aromatic hybrid rice Guangxiangyou 36 |
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111328699A (en) * | 2020-01-21 | 2020-06-26 | 江苏沿海地区农业科学研究所 | Breeding method of rice variety with purple black yellow glume seed coats |
| CN111328699B (en) * | 2020-01-21 | 2022-05-06 | 江苏沿海地区农业科学研究所 | Breeding method of rice variety with purple black yellow glume seed coats |
| CN111631129A (en) * | 2020-05-26 | 2020-09-08 | 上海市农业科学院 | Rapid breeding method of fragrant and soft hybrid japonica rice sterile line |
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