CN106613917B - A kind of selection of the complete female hua ' nan-type cucumber of white green - Google Patents
A kind of selection of the complete female hua ' nan-type cucumber of white green Download PDFInfo
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- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 69
- 244000299906 Cucumis sativus var. sativus Species 0.000 title 1
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 69
- 230000000306 recurrent effect Effects 0.000 claims abstract description 29
- 238000002955 isolation Methods 0.000 claims abstract description 26
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- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 120
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- 206010028980 Neoplasm Diseases 0.000 claims description 27
- 238000009395 breeding Methods 0.000 claims description 22
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- 241000894006 Bacteria Species 0.000 claims description 7
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- 238000012163 sequencing technique Methods 0.000 claims description 3
- 230000008774 maternal effect Effects 0.000 abstract description 20
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 abstract description 6
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract 1
- 241000223218 Fusarium Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 54
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 20
- 201000010099 disease Diseases 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 229910001961 silver nitrate Inorganic materials 0.000 description 10
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- 239000003292 glue Substances 0.000 description 6
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- 235000009852 Cucurbita pepo Nutrition 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- QDOXWKRWXJOMAK-UHFFFAOYSA-N dichromium trioxide Chemical compound O=[Cr]O[Cr]=O QDOXWKRWXJOMAK-UHFFFAOYSA-N 0.000 description 4
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- 244000183685 Citrus aurantium Species 0.000 description 1
- 235000007716 Citrus aurantium Nutrition 0.000 description 1
- 235000005976 Citrus sinensis Nutrition 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of selections of the white complete female hua ' nan-type cucumber of green, include the following steps：The female series kind obtained by selfing isolation and selection using purpleflower violet herb kind is nonrecurrent parent, the synoecy kind obtained by selfing isolation and selection using purpleflower violet herb kind is recurrent parent, the nonrecurrent parent and recurrent parent continuous 4~6 generation are returned, obtain backcross, the backcross is handed over by selfing or sisters, obtains female parent；It is male parent that dry land cucumber kind the raw kind on the 9th~13 section by the first female flower that 2~4 generations selfing isolation and selection obtains；By the maternal and paternal hybrid, the complete female hua ' nan-type cucumber of white green is obtained.The complete female hua ' nan-type cucumber of white green that the present invention cultivates is than emerald green volume increase 26.3~27.1%, thorn volume increase 22.6~23.5% closeer than Xintai City, and moderate resistance powdery mildew of cucumber, anti-cucumber downy mildew, moderate resistance cucumber fusarium axysporum and anti-Cucumber Target Leaf Spot.
Description
Technical field
The invention belongs to breeding method technical field more particularly to a kind of selection and breeding sides of the complete female hua ' nan-type cucumber of white green
Method.
Background technology
Cucumber is important gourd vegetable crop, and according to current statistics, national cucumber cultivation area reaches 963,000 hectares, I
The cucumber of state's cultivation mainly has North-China Type and South China type two major classes, and prevailing kind is mostly North-China Type currently on the market.
It is the Main Cultivation type in current China's production that the property type performance of cucumber, which has diversity, synoecy,.Female flower of cucumber
The height and density of section are the principal elements for influencing yield, complete female or strong female kind due to the not no nutrient competition of male flower and
Consumption, can increase substantially the economic flow rate of cucumber.It is to cultivate and produce height with gynoecy in cucumber heterosis utilization
Quality seeds reduce the major Seed company's realization kind monopolization power of seed costs maximally efficient technical measures and the world
Optimal path.Thus selection and breeding and creates and female be tied to form the technology focus mutually captured unexpectedly for breed cucumber entirely.
In the developed countries such as the U.S., Japan, Israel and West Europe, female line breeding technique has obtained in breed cucumber at present
It is utilized to extensive, is bred as and has promoted many kinds, produce economic results in society, it is domestic based on the cucumber research institute of Tianjin,
The kind for being bred as promoting is mostly the North-China Type of synoecy.And the domestic South China type Huang for being bred as and promoting and applying in production
Wide in variety based on outdoor cropping, the performance mostly non-gynoecy of melon, and it is more to implement fruit in the hua ' nan-type cucumber kind of cultivation
For bottle green or green variety, and the yield of the cucumber of these kinds is not high, such as the cucumber of emerald green kind, yield per acre
For 3650kg, the cucumber of the close thorn kind in Xintai City, yield per acre is 3762kg.
Invention content
In view of this, in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to and providing a kind of complete female south China of white green
The selection of type cucumber can improve the yield of cucumber.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
A kind of selection of the complete female hua ' nan-type cucumber of white green, includes the following steps：
1) the female series kind obtained by selfing isolation and selection using purpleflower violet herb kind is nonrecurrent parent, with purpleflower violet herb product
Kind is recurrent parent by the synoecy kind that selfing isolation and selection obtains, by the nonrecurrent parent and recurrent parent
Continuous 4~6 generation backcrossing, obtains backcross, and the backcross is handed over by selfing or sisters, obtains female parent；
2) dry land cucumber kind by the first female flower that 2~4 generations selfing isolation and selection obtains raw on the 9th~13 section
Kind is male parent；
3) it is yellow to obtain the complete female South China type of white green for the paternal hybrid for obtaining the female parent that the step 1) obtains with step 2)
Melon；
The step 1) and step 2) are limited without time sequencing.
Preferably, the first female flower of the female parent it is raw the 5th~7 section is upper, melon grow 16~20cm, melon transverse diameter 2.5~
4cm, thorn tumor, melon weigh 120~160g, downy mildew resistance, anthracnose and powdery mildew in vain.
Preferably, the first female flower of the male parent life on the 9th~13 section, melon 14~18cm of length, and melon transverse diameter 2.5~
4.5cm pierces tumor, downy mildew resistance, anthracnose and powdery mildew in vain.
Preferably, the first female flower of the nonrecurrent parent in the step 1) raw on the 2nd~3 section, and melon length 20~
25cm, melon 2~4cm of transverse diameter pierce tumor, bacteria resistance angular leaf spot, powdery mildew and downy mildew in vain.
Preferably, the dry land cucumber kind in the step 2) is the dry land cucumber kind white jade spring.
Preferably, the algebraically being selfed when recurrent parent described in selection and breeding in the step 1) was 3~5 generations.
The present invention provides a kind of selections of the white complete female hua ' nan-type cucumber of green, include the following steps：With purpleflower violet herb
Kind is nonrecurrent parent by the female series kind that selfing isolation and selection obtains, with purpleflower violet herb kind by selfing isolation and selection
Obtained synoecy kind is recurrent parent, and the nonrecurrent parent and recurrent parent continuous 4~6 generation are returned, obtained
To backcross, the backcross is handed over by selfing or sisters, obtains female parent；By dry land cucumber kind by the selfing point of 2~4 generations
It is male parent that the first female flower obtained from selection and breeding, which the raw kind on the 9th~13 section,；By the maternal and paternal hybrid, obtain white
Green complete female hua ' nan-type cucumber.Female parent selection be high-yield disease resisting gynoecy kind, male parent selection be the strong kind of disease resistance,
Therefore the filial generation high-yield disease resisting obtained.The white complete female hua ' nan-type cucumber of green that the present invention cultivates than emerald green volume increase 26.3~
27.1%, thorn volume increase 22.6~23.5% closeer than Xintai City, and also moderate resistance powdery mildew of cucumber, anti-cucumber downy mildew, moderate resistance cucumber are withered
Disease of withering and anti-Cucumber Target Leaf Spot.
Specific implementation mode
The present invention provides a kind of selections of the white complete female hua ' nan-type cucumber of green, include the following steps：
1) the female series kind obtained by selfing isolation and selection using purpleflower violet herb kind is nonrecurrent parent, with purpleflower violet herb product
Kind is recurrent parent by the synoecy kind that selfing isolation and selection obtains, by the nonrecurrent parent and recurrent parent
Continuous 4~6 generation backcrossing, obtains backcross, and the backcross is handed over by selfing or sisters, obtains female parent；
2) dry land cucumber kind by the first female flower that 2~4 generations selfing isolation and selection obtains raw on the 9th~13 section
Kind is male parent；
3) it is yellow to obtain the complete female South China type of white green for the paternal hybrid for obtaining the female parent that the step 1) obtains with step 2)
Melon；
The step 1) and step 2) are limited without time sequencing.
In the present invention, the nonrecurrent parent is to be selfed the female series product that isolation and selection obtains by purpleflower violet herb kind
Kind.The purpleflower violet herb kind is preferably the kind " glue county purpleflower violet herb " from Jiaozhou City, and farmers' reserves seed for planting certainly.Described " glue county
Cucumber " obtains nonrecurrent parent preferably through 2~4 generations selfing isolation and selection.Two single plants that glue county purpleflower violet herb is selected and remain for 1996,
The strong female of 96481-1 performances.The canopy 96481-1 of spring in 1997 transplants 24 plants altogether, and performance is complete female, carries out drug (silver nitrate) and handles,
Pollination self, which is carried out, to each plant mixes harvesting.Autumn canopy plantation 96481-1- in 1997 therefrom selects 3 plants of progress earlier from mixing
Selfing is reserved seed for planting.For plantation observation in 1998 as a result, 96481-1- is almost the same from mixed -1 performance, field growing performance is more frost-resistant mould
Disease, bacteria resistance angular leaf spot and droop are to get to nonrecurrent parent.
In the present invention, the character of the nonrecurrent parent is preferably mid-early maturity, and the first female flower life on the 2nd~3 section,
Growth potential is medium, medium blade size, sugar-preserved gourd bottle green, white to pierce that tumor is small and rare, even thickness, in flavor quality on, resist thin
Bacterium property angular leaf spot, powdery mildew and downy mildew.The nonrecurrent parent melon length be preferably 20~25cm, more preferably 22~
24cm, most preferably 23cm.The melon transverse diameter of the nonrecurrent parent is preferably 2~4cm, more preferably 3cm.
In the present invention, the recurrent parent is the synoecy that purpleflower violet herb kind is obtained by selfing isolation and selection
Kind.The purpleflower violet herb kind is preferably the kind " Suizhong purpleflower violet herb " introduced from Suizhong County." the Suizhong purpleflower violet herb " is preferred
Recurrent parent is obtained by 3~5 generations selfing isolation and selection, it is isolated more preferably to pass through the selfing of 4 generations.Suizhong purpleflower violet herb 1982
Year carries out self cross and selection, and the 7th plant of performance is excellent, and the 82 Huang 4-7 selected and remain, which continue to plant to observe, carries out self cross and selection, and the therein 3rd
Strain performance is excellent.Then it plants and observes spring in 1999, continue selfing separation, the 82 big portions Huang 4-7-3-1 of observation result
It is light green color to divide plant pericarp, 2 plants of not homophyletics occurs, the short melon of fruit properties performance, white skin, white tumor of piercing are rare, plant strain growth table
It is now normal, it carries out selfing and reserves seed for planting.To 2 strains " 82 Huang 4-7-3-1-2 " selected and remain and " 82 Huang 4-7-3-1-3 " in the same year in the autumn
Plantation observation is carried out under the conditions of greenhouse delayed cultivation.The growing way performance of " 82 Huang 4-7-3-1-3 " is wanted compared with " 82 Huang 4-7-3-1-2 "
Prosperous, maturity is also relatively early." 82 Huang 4-7-3-1-3 " strain is observed and reserved seed for planting to get to wheel in spring plastic green house in 2000
Return parent.
In the present invention, the target of the recurrent parent selection and breeding is preferably late-maturing, melon yellow-white, melon tip circle, and white thorn tumor is small
And it is rare, meat thickness accounts for the 60~70% of melon transverse diameter.First female flower of the recurrent parent preferably it is raw on the 10th~13 section, more
It is preferred that it is raw on 11.4 sections.Melon length preferably 14~18cm, the more preferably 15~17cm of the recurrent parent, most preferably
16cm.The melon transverse diameter of the recurrent parent is preferably 2~5cm, more preferably 3~4cm, most preferably 3.4cm.
After obtaining recurrent parent and nonrecurrent parent, the nonrecurrent parent and recurrent parent are passed through continuous 4 by the present invention
In~6 generations, were returned, and obtained backcross.After obtaining backcross, the present invention hands over the backcross by selfing or sisters, obtains
To female parent.In the present invention, described be selfed is preferably：Full female plant in the backcross is handled by silver nitrate aqueous solution
Afterwards, it is selfed, self progeny selects the unseparated full female plant of self progeny's property spent, as female parent by identification.In the present invention
In, the mass concentration of the silver nitrate aqueous solution is preferably 0.02~0.04%, and more preferably 0.025~0.035%, most preferably
It is 0.03%.In the present invention, the silver nitrate processing can induce full female plant to generate male flower, then be selfed.In the present invention
In, the processing method of the silver nitrate solution is specially：The silver nitrate for being 0.03% with mass concentration is sprayed in 41 heart of leaf of cucumber
Blade face is spilt, even spray 3 times, every minor tick 3-5 days.
In the present invention, the principle of the maternal selection and breeding is：Full female plant and common cultivation kind (synoecy) are yellow
What melon spent property is not both the difference due to one pair of genes, and the breeding of gynoecy strain can utilize the method for backcross transformation will be complete
Female is transferred in common variety, then using continuous 5~6 backcrossings and selection, until last time backcross generations,
Elected full female plant selfing generates full female plant and male and female that homogeneity combines (FF) and heterogeneous combination (Ff) in the offspring of selfing
The separation of three kinds of different genotype of different colored homophyletic (ff), first eliminates non-full female plant, and homogeneity combines (FF) or heterogeneous combination
(Ff) full female plant is not easy to distinguish in appearance, it is necessary to be handled with silver nitrate, selfing is reserved seed for planting respectively again for each strain, is identified through offspring
It could differentiate.If homogeneity combine full female plant, thereafter seville orange flower property will not detach again, the offspring of the full female plant of heterogeneous combination then can
Row separation again.Then the full female plant of heterogeneous combination that can be detached after identified is eliminated, retains the full female plant that the homogeneity of incubation combines
Cucumis sativus line, it is as maternal.
Maternal breeding process is shown in Table 1：
In the present invention, the character of the female parent is preferably that plant growing way is strong, leaf green, median size, in branch, maturity
In, melon yellow-white, short cylinder shape, both ends are neat, white to pierce tumor rareness, median size, downy mildew resistance, anthracnose and powdery mildew.It is described
The first maternal female flower preferably it is raw on the 5th~7 section, more preferably it is raw on Section 6.2.The melon of the female parent is grown
16~20cm, more preferably 17.5~19cm, most preferably 18.4cm.The melon transverse diameter of the female parent is preferably 2.5~4cm, more
Preferably 3~3.5cm, most preferably 3.3cm.The melon of the female parent weighs 120~160g, more preferably 130~150g, most preferably
For 140g.
In the present invention, the male parent is that dry land cucumber kind is selfed the first female flower that isolation and selection obtains by 2~4 generations
The raw kind on the 9th~13 section, more preferably passes through 3 generations selfing isolation and selection and obtains.In the present invention, the male parent choosing
It is preferably that plant strain growth gesture is strong and tolerance to low temperature and weak light to educate target.The leaf color of the male parent is shallow, and in branch, melon yellow green pierces tumor in vain
Less, big in, melon both ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew.The melon length of the male parent is preferably 14~18cm,
More preferably 15.5~17cm, most preferably 16.5cm.The melon transverse diameter of the male parent is preferably 2.5~4.5cm, more preferably
2.8~3.5cm, most preferably 3cm.The melon of the male parent again be preferably 120~140g, more preferably 125~135g, most preferably
For 132g.
The present invention is not particularly limited the kind of the dry land cucumber, be preferably derived from Northeast gardening local dry land cucumber
The kind white jade spring (II).
After obtaining maternal and male parent, the maternal and paternal hybrid is obtained the complete female hua ' nan-type cucumber of white green by the present invention.
In the present invention, the character of the complete female hua ' nan-type cucumber of white green is preferably complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain
Color, the smooth no rib ditch in melon surface, white to pierce that tumor is few, median size, crisp in taste, plant strain growth gesture is strong, and melon is precocious, mildew-resistance,
Downy mildew, droop and brown spot.The melon length of the white complete female hua ' nan-type cucumber of green is preferably 16~20cm, and more preferably 17
~19cm, most preferably 18cm；First female flower of the white complete female hua ' nan-type cucumber of green preferably it is raw on the 2nd~4 section, more
It is preferred that it is raw in Section 3；The melon of the complete female hua ' nan-type cucumber of white green is preferably 2~3cm, more preferably 2.8cm；Institute
The melon for stating the complete female hua ' nan-type cucumber of white green is preferably 120~150g, more preferably 125~140g, most preferably 133.7g again；
The transverse diameter of the complete female hua ' nan-type cucumber of white green is preferably 2.5~4.5cm, more preferably 3~4.0cm, most preferably
3.3cm。
The present invention is not particularly limited the cultural method of the white complete female hua ' nan-type cucumber of green, preferably ridge is used to plant
Mode, wide row space 70~90cm, small 30~50cm of line-spacing, 20~40cm of spacing in the rows, planting density are 3500~4000 plants/acre；More
Preferably 75~85cm of wide row space, small 35~45cm of line-spacing, 22~35cm of spacing in the rows, planting density are 3600~3900 plants/acre；Most
Preferably wide row space 80cm, small line-spacing 40cm, 25~30cm of spacing in the rows, planting density are 3700~3800 plants/acre.
When using large-scale attached-greenhouse cultivation, planting density is preferably 1600~2000 plants/acre, more preferably 1700~
1900 plants/acre, most preferably 1800 plants/acre.
It is detailed to a kind of selection progress of the white complete female hua ' nan-type cucumber of green provided by the invention with reference to embodiment
Thin explanation, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
1) maternal selection and breeding：
Glue county purpleflower violet herb goes out nonrecurrent parent by 3 generations selfing isolation and selection, and the character of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 2, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn tumor is small and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 23cm of melon, melon transverse diameter 3cm.
Suizhong purpleflower violet herb obtains recurrent parent by 4 generations selfing isolation and selection, and the character of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white tumor of piercing is small and rare, and meat thickness accounts for the 65% of melon transverse diameter, the first female flower preferably it is raw on Section 11.4, melon is long
16cm, melon transverse diameter 3.4cm.
Nonrecurrent parent is returned with recurrent parent by continuous 5 generation, obtains backcross, eliminates non-full female plant, is left complete
Female plant, full female plant are handled by the silver nitrate solution that mass concentration is 0.03%, and induction generates male flower, and full female plant selfing is eliminated
The full female plant of self progeny's property spent separation, it is female parent to leave the unseparated full female plant of colored property.Maternal character is plant growing way
By force, leaf green, median size, in branch, in maturity, melon yellow-white, short cylinder shape, both ends are neat, and white to pierce tumor rare, medium big
Small, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw on Section 6.2, the long 18.4cm of melon, melon transverse diameter
3.3cm, melon weight 140g.
2) male parent selection and breeding：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening by the selfing point of 3 generations
Male parent is obtained from selection and breeding, and it is raw in Section 10 that the character of male parent is that the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, it is white pierce tumor it is few, in it is big, melon both ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 16.5cm, melon transverse diameter 3cm, melon weight 132g.
3) selection and breeding of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, it is yellow to obtain the complete female South China type of white green
Melon, character are complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, the smooth no rib ditch in melon surface, white to pierce that tumor is few, median size,
Crisp in taste, plant strain growth gesture is strong, and melon is precocious, the long 18cm of melon, and the first female flower life in Section 3, and melon is 2.8cm, melon weight
133.7g, melon transverse diameter 3.3cm.
Embodiment 2
1) maternal selection and breeding：
Glue county purpleflower violet herb goes out nonrecurrent parent by 2 generations selfing isolation and selection, and the character of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 3, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn tumor is small and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 20cm of melon, melon transverse diameter 4cm.
Suizhong purpleflower violet herb obtains recurrent parent by 3 generations selfing isolation and selection, and the character of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white tumor of piercing is small and rare, and meat thickness accounts for the 60% of melon transverse diameter, the first female flower preferably it is raw in Section 10, melon is long
17cm, melon transverse diameter 3cm.
Nonrecurrent parent is returned with recurrent parent by continuous 6 generation, obtains backcross, and backcross passes through mass concentration
It is handled for 0.02% silver nitrate solution, superseded staminiferous plant leaves female plant, and female plant obtains female parent by selfing.Maternal character is
Plant growing way is strong, leaf green, median size, and in branch, in maturity, melon yellow-white, short cylinder shape, both ends are neat, white thorn tumor rareness,
Median size, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw in Section 5, the long 16cm of melon, melon transverse diameter
3cm, melon weight 150g.
2) male parent selection and breeding：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening by the selfing point of 3 generations
Male parent is obtained from selection and breeding, and it is raw in Section 9 that the character of male parent is that the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, it is white pierce tumor it is few, in it is big, melon both ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 16cm, melon transverse diameter 2.5cm, melon weight 125g.
3) selection and breeding of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, it is yellow to obtain the complete female South China type of white green
Melon, character are complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, the smooth no rib ditch in melon surface, white to pierce that tumor is few, median size,
Crisp in taste, plant strain growth gesture is strong, and melon is precocious, the long 19cm of melon, and the first female flower life in Section 3, and melon is 2.5cm, melon weight
140g, melon transverse diameter 3cm.
Embodiment 3
1) maternal selection and breeding：
Glue county purpleflower violet herb goes out nonrecurrent parent by 4 generations selfing isolation and selection, and the character of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 3, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn tumor is small and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 24cm of melon, melon transverse diameter 2.5cm.
Suizhong purpleflower violet herb obtains recurrent parent by 3 generations selfing isolation and selection, and the character of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white tumor of piercing is small and rare, and meat thickness accounts for the 70% of melon transverse diameter, the first female flower preferably it is raw in Section 12, melon is long
18cm, melon transverse diameter 4.5cm.
Nonrecurrent parent is returned with recurrent parent by continuous 6 generation, obtains backcross, and backcross passes through mass concentration
It is handled for 0.04% silver nitrate solution, superseded staminiferous plant leaves female plant, and female plant obtains female parent by selfing.Maternal character is
Plant growing way is strong, leaf green, median size, and in branch, in maturity, melon yellow-white, short cylinder shape, both ends are neat, white thorn tumor rareness,
Median size, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw in Section 7, the long 17.5cm of melon, and melon is horizontal
Diameter 2.5cm, melon weight 145g.
2) male parent selection and breeding：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening by the selfing point of 3 generations
Male parent is obtained from selection and breeding, and it is raw in Section 9 that the character of male parent is that the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, it is white pierce tumor it is few, in it is big, melon both ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 17cm, melon transverse diameter 3.5cm, melon weight 140g.
3) selection and breeding of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, it is yellow to obtain the complete female South China type of white green
Melon, character are complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, the smooth no rib ditch in melon surface, white to pierce that tumor is few, median size,
Crisp in taste, plant strain growth gesture is strong, and melon is precocious, the long 19cm of melon, and the first female flower, which, gives birth in Section 3, and 3cm, melon are weighed 135g by melon,
Melon transverse diameter 3.5cm.
Embodiment 4
The complete female hua ' nan-type cucumber of white green that Examples 1 to 3 obtains is colonized, site preparation is carried out before being colonized, applies per acre
Decomposed manure 8000kg, calcium superphosphate 50kg, N, P, K composite fertilizer 50kg, is planted using ridge, wide row space 80cm, small line-spacing 40cm,
Spacing in the rows 30cm, planting density are 3500 plants/acre.Routine Management method management is used after field planting, with the emerald green and close thorn kind in Xintai City
For control, 1 the results are shown in Table：
The yield of the complete female hua ' nan-type cucumber of the white green of 1 Examples 1 to 3 of table
As can be drawn from Table 1, the white complete female hua ' nan-type cucumber of green that the present invention cultivates than emerald green volume increase 26.3~
27.1%, thorn volume increase closeer than Xintai City 22.6~23.5%.
It is identified through Institute of Vegetables, Chinese Academy of Agricultural Sciences, the complete female anti-yellowing melon of hua ' nan-type cucumber of white green that the present invention cultivates
It is 49.68 that powdery mildew disease, which refers to, belongs to moderate resistance grade；It is 31.30 that anti-cucumber downy mildew disease, which refers to, belongs to disease-resistant grade；Anti-yellowing melon is withered
It is 34.58 that disease of withering disease, which refers to, belongs to moderate resistance grade；It is 20.50 that anti-Cucumber Target Leaf Spot disease, which refers to, belongs to disease-resistant grade.
Can be obtained by above example, the white complete female hua ' nan-type cucumber of green that the present invention cultivates than emerald green volume increase 26.3~
27.1%, thorn volume increase 22.6~23.5% closeer than Xintai City, and also moderate resistance powdery mildew of cucumber, anti-cucumber downy mildew, moderate resistance cucumber are withered
Disease of withering and anti-Cucumber Target Leaf Spot.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of selection of the complete female hua ' nan-type cucumber of white green, comprises the steps of：
1) the female series kind obtained by selfing isolation and selection using purpleflower violet herb kind is passed through as nonrecurrent parent with purpleflower violet herb kind
It is recurrent parent to cross the synoecy kind that selfing isolation and selection obtains, and the nonrecurrent parent and recurrent parent is continuous
In 4~6 generations, were returned, and obtained backcross, and the backcross is handed over by selfing or sisters, obtains female parent；
2) dry land cucumber kind into the raw kind on the 9th~13 section by the first female flower that 2~4 generations selfing isolation and selection obtains
For male parent；
3) paternal hybrid for obtaining the female parent that the step 1) obtains with step 2) obtains the complete female hua ' nan-type cucumber of white green；
The step 1) and step 2) are limited without time sequencing；
First female flower of the female parent life and saves upper, 16~20cm of melon length, 2.5~4cm of melon transverse diameter, in vain thorn tumor, melon the 5th~7
Weigh 120~160g, downy mildew resistance, anthracnose and powdery mildew；
First female flower of the male parent life on the 9th~13 section, and melon grows 14~18cm, and melon 2.5~4.5cm of transverse diameter pierces tumor in vain,
Melon weighs 120~140g, downy mildew resistance, anthracnose and powdery mildew；
First female flower of the nonrecurrent parent in the step 1) life on the 2nd~3 section, melon 20~25cm of length, and melon transverse diameter 2~
4cm pierces tumor, bacteria resistance angular leaf spot, powdery mildew and downy mildew in vain.
2. selection according to claim 1, which is characterized in that the dry land cucumber kind in the step 2) is dry land cucumber
The kind white jade spring.
3. selection according to claim 1, which is characterized in that in the step 1) described in selection and breeding when recurrent parent from
The algebraically of friendship was 3~5 generations.
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