CN106613917A - Breeding method for white green all-female Huanan-type cucumber - Google Patents
Breeding method for white green all-female Huanan-type cucumber Download PDFInfo
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- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 77
- 238000009395 breeding Methods 0.000 title abstract description 9
- 244000299906 Cucumis sativus var. sativus Species 0.000 title 1
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 77
- 230000000306 recurrent effect Effects 0.000 claims abstract description 29
- 241000233679 Peronosporaceae Species 0.000 claims abstract description 22
- 241000221785 Erysiphales Species 0.000 claims abstract description 19
- 241000219112 Cucumis Species 0.000 claims description 122
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 122
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 122
- 230000008774 maternal effect Effects 0.000 claims description 25
- 244000137773 Viola philippica Species 0.000 claims description 23
- 238000002955 isolation Methods 0.000 claims description 23
- 201000010099 disease Diseases 0.000 claims description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 13
- 230000008775 paternal effect Effects 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000010977 jade Substances 0.000 claims description 6
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims 1
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 238000005204 segregation Methods 0.000 abstract 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract 1
- 241000223218 Fusarium Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 52
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 20
- 244000265913 Crataegus laevigata Species 0.000 description 19
- 235000013175 Crataegus laevigata Nutrition 0.000 description 19
- 229910001961 silver nitrate Inorganic materials 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 239000003292 glue Substances 0.000 description 6
- 241000219122 Cucurbita Species 0.000 description 5
- 235000009852 Cucurbita pepo Nutrition 0.000 description 5
- 240000000400 Balanites maughamii Species 0.000 description 4
- QDOXWKRWXJOMAK-UHFFFAOYSA-N dichromium trioxide Chemical compound O=[Cr]O[Cr]=O QDOXWKRWXJOMAK-UHFFFAOYSA-N 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 238000010413 gardening Methods 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000004383 yellowing Methods 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 244000183685 Citrus aurantium Species 0.000 description 1
- 235000007716 Citrus aurantium Nutrition 0.000 description 1
- 235000005976 Citrus sinensis Nutrition 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009405 line breeding Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a breeding method for white green all-female Huanan-type cucumber. The breeding method for white green all-female Huanan-type cucumber comprises the following steps: using a female variety obtained by self-segregation breeding of a land cucumber variety as a nonrecurrent parent, and using a monoecious variety obtained by the self-segregation breeding of the land cucumber variety as a recurrent parent, and continuously carrying out 4-6 generations of the backcross to the nonrecurrent parent and the recurrent parent, obtaining a backcross variety, and obtaining a female parent by the selfing or sister cross of the backcross variety; using a variety, of which the first female flower is grown on the ninth-thirteenth knobs, obtained by 2-4 generations of the self-segregation breeding of a dry land cucumber variety as a male parent; hybridizing the female parent and the male parent to obtain the white green all-female Huanan-type cucumber. Compared with the emerald green cucumber, the yield of the white green all-female Huanan-type cucumber is improved by 26.3-27.1%; and compared with the Xintai Mici cucumber, the yield is improved by 22.6-23.5%. The white green all-female Huanan-type cucumber has the characteristics of cucumber powdery mildew moderate resistance, cucumber downy mildew resistance, cucumber fusarium wilt moderate resistance and cucumber target leaf spot resistance.
Description
Technical field
The invention belongs to breeding method technical field, more particularly to a kind of seed selection side of the complete female hua ' nan-type cucumber of white green
Method.
Background technology
Cucumber is important gourd vegetable crop, and according to statistics at present, national cucumber cultivation area reaches 963,000 hectares, I
The cucumber of state's cultivation mainly has North-China Type and the big class of South China type two, and currently on the market prevailing kind mostly is North-China Type.
The property type performance of cucumber has diversity, and synoecy is the Main Cultivation type in current China's production.Female flower of cucumber
The height and density of section position is the principal element for affecting yield, complete female or strong female kind due to the nutrient competition without male flower with
Consume, the economic flow rate of cucumber can be increased substantially.It is to cultivate and produce height with gynoecy in cucumber heterosis utilization
The maximally efficient technical measures of quality seeds, reduction seed costs, are also that Ge great Seed companies of the world realize kind monopolization power
Optimal path.Thus seed selection with create the complete female technology focus mutually captured unexpectedly for breed cucumber of being tied to form.
At present in developed countries such as the U.S., Japan, Israel and West Europe, female line breeding technique is obtained in breed cucumber
To extensively utilization, many kinds are bred as and have been promoted, generate economic results in society, it is domestic based on the cucumber research institute of Tianjin,
The kind for being bred as popularization mostly is the North-China Type of synoecy.And the South China type of domestic incubation and popularization and application aborning is yellow
Wide in variety based on outdoor cropping, the performance mostly non-gynoecy of melon, and it is more to implement fruit in the hua ' nan-type cucumber kind of cultivation
For bottle green or green variety, and the yield of the cucumber of these kinds is not high, such as the cucumber of emerald green kind, the yield on per mu of ground
For 3650kg, the cucumber of the close thorn kind in Xintai City, the yield on per mu of ground is 3762kg.
The content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide a kind of white green complete female south China
The selection of type cucumber, it is possible to increase the yield of cucumber.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
A kind of selection of white green complete female hua ' nan-type cucumber, comprises the following steps：
1) the female series kind obtained through selfing isolation and selection with purpleflower violet herb kind is as nonrecurrent parent, with purpleflower violet herb product
It is recurrent parent to plant the synoecy kind obtained through selfing isolation and selection, by the nonrecurrent parent and recurrent parent
Continuous 4～6 generation backcrossing, obtains backcross, and the backcross is handed over through selfing or sisters, obtains female parent；
2) dry land cucumber kind through the first female flower that 2～4 generations selfing isolation and selection is obtained raw on the 9th～13 section
Kind is male parent；
3) by the step 1) female parent that obtains and step 2) paternal hybrid that obtains, obtain the complete female South China type of white green yellow
Melon；
The step 1) and step 2) limit without time sequencing.
Preferably, the first maternal female flower it is raw on the 5th～7 section, melon 16～20cm of length, melon transverse diameter 2.5～
4cm, in vain thorn knurl, melon weigh 120～160g, downy mildew resistance, anthracnose and powdery mildew.
Preferably, the first female flower of the male parent life on the 9th～13 section, melon 14～18cm of length, and melon transverse diameter 2.5～
4.5cm, pierces in vain knurl, downy mildew resistance, anthracnose and powdery mildew.
Preferably, the step 1) in nonrecurrent parent the first female flower it is raw on the 2nd～3 section, melon length 20～
25cm, melon 2～4cm of transverse diameter, pierce in vain knurl, bacteria resistance angular leaf spot, powdery mildew and downy mildew.
Preferably, the step 2) in dry land cucumber kind be the dry land cucumber kind white jade spring.
Preferably, the step 1) in described in seed selection during recurrent parent selfing algebraically be 3～5 generations.
The invention provides a kind of selection of white green complete female hua ' nan-type cucumber, comprises the following steps：With purpleflower violet herb
Kind is nonrecurrent parent through the female series kind that selfing isolation and selection is obtained, with purpleflower violet herb kind through selfing isolation and selection
The synoecy kind for obtaining is recurrent parent, and the nonrecurrent parent and continuous 4～6 generation backcrossing of recurrent parent are obtained
To backcross, the backcross is handed over through selfing or sisters, obtains female parent；By dry land cucumber kind through 2～4 generations selfing point
It is male parent that the first female flower obtained from seed selection the raw kind on the 9th～13 section；By the maternal and paternal hybrid, obtain white
Green complete female hua ' nan-type cucumber.Female parent selection be high-yield disease resisting gynoecy kind, male parent select be the strong kind of disease resistance,
Therefore the filial generation high-yield disease resisting for obtaining.The complete female hua ' nan-type cucumber of white green that the present invention is cultivated than emerald green volume increase 26.3～
27.1%, it is closeer than Xintai City thorn volume increase 22.6～23.5%, and in anti-powdery mildew of cucumber, anti-cucumber downy mildew, in anti-yellowing melon it is withered
Disease of withering and anti-Cucumber Target Leaf Spot.
Specific embodiment
The invention provides a kind of selection of white green complete female hua ' nan-type cucumber, comprises the following steps：
1) the female series kind obtained through selfing isolation and selection with purpleflower violet herb kind is as nonrecurrent parent, with purpleflower violet herb product
It is recurrent parent to plant the synoecy kind obtained through selfing isolation and selection, by the nonrecurrent parent and recurrent parent
Continuous 4～6 generation backcrossing, obtains backcross, and the backcross is handed over through selfing or sisters, obtains female parent；
2) dry land cucumber kind through the first female flower that 2～4 generations selfing isolation and selection is obtained raw on the 9th～13 section
Kind is male parent；
3) by the step 1) female parent that obtains and step 2) paternal hybrid that obtains, obtain the complete female South China type of white green yellow
Melon；
The step 1) and step 2) limit without time sequencing.
In the present invention, the nonrecurrent parent is the female series product obtained through purpleflower violet herb kind selfing isolation and selection
Kind.The purpleflower violet herb kind preferably from the kind " glue county purpleflower violet herb " of Jiaozhou City, reserve seed for planting certainly by farmers'." the glue county ground
Cucumber " obtains nonrecurrent parent preferably through 2～4 generations selfing isolation and selection.Two individual plants that glue county purpleflower violet herb is selected and remain for 1996,
The strong female of 96481-1 performances.Spring in 1997, canopy 96481-1 transplanted altogether 24 plants, and performance is complete female, carried out medicine (silver nitrate) process,
Pollination self is carried out to each strain and mixes harvesting.The plantation 96481-1- of autumn canopy in 1997 from mixed, therefrom select earlier 3 plants carry out
Selfing is reserved seed for planting.Plantation observation result in 1998,96481-1- is basically identical from mixed -1 performance, and field growing performance is more frost-resistant mould
Disease, bacteria resistance angular leaf spot and droop, that is, obtain nonrecurrent parent.
In the present invention, the proterties of the nonrecurrent parent is preferably mid-early maturity, and the first female flower life on the 2nd～3 section,
Growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn knurl is little and rare, even thickness, in flavor quality on, resist thin
Bacterium property angular leaf spot, powdery mildew and downy mildew.The melon length of the nonrecurrent parent is preferably 20～25cm, more preferably 22～
24cm, most preferably 23cm.The melon transverse diameter of the nonrecurrent parent is preferably 2～4cm, more preferably 3cm.
In the present invention, the recurrent parent is the synoecy that purpleflower violet herb kind is obtained through selfing isolation and selection
Kind.The purpleflower violet herb kind is preferably the kind " Suizhong purpleflower violet herb " introduced from Suizhong County." the Suizhong purpleflower violet herb " is preferred
Recurrent parent is obtained through 3～5 generations selfing isolation and selection, it is more preferably isolated through the selfing of 4 generations.Suizhong purpleflower violet herb 1982
Year self cross and selection is carried out, the 7th plant of performance is excellent, the 82 Huang 4-7 continuation plantation observations selected and remain carry out self cross and selection, the therein 3rd
Strain performance is excellent.Then spring in 1999, plantation was observed, and proceeded selfing separation, the big portions of the observation Huang 4-7-3-1 of result 82
Divide plant pericarp to be light green color, 2 plants of not homophyletics occur, the short melon of fruit properties performance, white skin, white knurl of piercing are rare, plant strain growth table
It is now normal, carry out selfing and reserve seed for planting.To 2 strains " 82 Huang 4-7-3-1-2 " for selecting and remain and " 82 Huang 4-7-3-1-3 " in the same year in the autumn
Plantation observation is carried out under the conditions of booth delayed cultivation.The growing way performance of " 82 Huang 4-7-3-1-3 " will compared with " 82 Huang 4-7-3-1-2 "
Prosperous, maturity is also more early.In spring plastic green house in 2000 " 82 Huang 4-7-3-1-3 " strain is observed and reserved seed for planting, that is, taken turns
Return parent.
In the present invention, the target of the recurrent parent seed selection is preferably late-maturing, melon yellow-white, melon tip circle, and white thorn knurl is little
And it is rare, meat thickness accounts for the 60～70% of melon transverse diameter.First female flower of the recurrent parent preferably it is raw on the 10th～13 section, more
It is preferred that it is raw on 11.4 sections.The melon length of the recurrent parent is preferably 14～18cm, more preferably 15～17cm, most preferably
16cm.The melon transverse diameter of the recurrent parent is preferably 2～5cm, most preferably more preferably 3～4cm, 3.4cm.
After obtaining recurrent parent and nonrecurrent parent, the present invention is by the nonrecurrent parent and recurrent parent through continuous 4
In～6 generations, were returned, and obtained backcross.After obtaining backcross, the present invention hands over the backcross through selfing or sisters, obtains
To female parent.In the present invention, the selfing is preferably：By the full female plant in the backcross through silver nitrate aqueous solution process
Afterwards, selfing is carried out, self progeny selects the unseparated full female plant of self progeny's property spent, as female parent through identification.In the present invention
In, the mass concentration of the silver nitrate aqueous solution is preferably 0.02～0.04%, more preferably 0.025～0.035%, most preferably
For 0.03%.In the present invention, the silver nitrate is processed can induce full female plant to produce male flower, then carry out selfing.In the present invention
In, the processing method of the silver nitrate solution is specially：Sprayed in 41 heart of leaf of cucumber with the silver nitrate that mass concentration is 0.03%
Blade face is spilt, even spray 3 times, every minor tick 3-5 days.
In the present invention, the principle of the maternal seed selection is：Full female plant and common cultivation kind (synoecy) are yellow
The difference not being both due to one pair of genes of melon flower property, therefore the breeding of gynoecy strain can utilize the method for backcross transformation by entirely
Female is transferred in common variety, then backcrossing and selection again through continuous 5～6 times, until last time backcross generations,
Elected full female plant selfing, in the offspring of selfing, produces full female plant and male and female that homogeneity combines (FF) and heterogeneous combination (Ff)
The separation of three kinds of different genotype of different colored homophyletic (ff), first eliminates non-full female plant, and homogeneity combines (FF) or heterogeneous combination
(Ff) full female plant is not easy to distinguish in appearance, it is necessary to which with silver nitrate process, again respectively selfing is reserved seed for planting for each strain, Jing offsprings identification
Could differentiate.If the full female plant that homogeneity is combined, thereafter seville orange flower property will not be separated again, and the offspring of the full female plant of heterogeneous combination then can
Again row is separated.Then after identified can the full female plant of detached heterogeneous combination eliminate, retain the full female plant that the homogeneity being bred as is combined
Cucumis sativus line, it is as maternal.
Maternal breeding process is shown in Table 1：
In the present invention, the maternal proterties is preferably plant growing way by force, leaf green, median size, in branch, maturity
In, melon yellow-white, short cylinder shape, two ends are neat, and white thorn knurl is rare, median size, downy mildew resistance, anthracnose and powdery mildew.It is described
The first maternal female flower preferably it is raw on the 5th～7 section, more preferably it is raw on Section 6.2.The maternal melon length is preferably
16～20cm, more preferably 17.5～19cm, most preferably 18.4cm.The maternal melon transverse diameter is preferably 2.5～4cm, more
Preferably 3～3.5cm, most preferably 3.3cm.The maternal melon weighs 120～160g, more preferably 130～150g, most preferably
For 140g.
In the present invention, the male parent is the first female flower for obtaining dry land cucumber kind through 2～4 generations selfing isolation and selection
The raw kind on the 9th～13 section, is more preferably obtained through 3 generations selfing isolation and selection.In the present invention, the male parent choosing
Educate target and be preferably plant strain growth gesture by force and tolerance to low temperature and weak light.The leaf color of the male parent is shallow, and in branch, melon yellow green pierces in vain knurl
Less, big in, melon two ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew.The melon length of the male parent is preferably 14～18cm,
More preferably 15.5～17cm, most preferably 16.5cm.The melon transverse diameter of the male parent is preferably 2.5～4.5cm, more preferably
2.8～3.5cm, most preferably 3cm.The melon of the male parent is preferably 120～140g, more preferably 125～135g again, most preferably
For 132g.
The present invention is not particularly limited to the kind of the dry land cucumber, be preferably derived from Northeast gardening local dry land cucumber
The kind white jade spring (II).
After obtaining maternal and male parent, the maternal and paternal hybrid is obtained the complete female hua ' nan-type cucumber of white green by the present invention.
In the present invention, the proterties of the complete female hua ' nan-type cucumber of the white green is preferably complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain
Color, melon surface is smooth without rib ditch, and white thorn knurl is few, median size, and mouthfeel is tender and crisp, and plant strain growth gesture is strong, and melon is precocious, mildew-resistance,
Downy mildew, droop and brown spot.The melon length of the complete female hua ' nan-type cucumber of the white green is preferably 16～20cm, and more preferably 17
～19cm, most preferably 18cm；First female flower of the complete female hua ' nan-type cucumber of the white green preferably it is raw on the 2nd～4 section, more
It is preferred that it is raw in Section 3；The melon of the white green complete female hua ' nan-type cucumber is preferably 2～3cm, more preferably 2.8cm；Institute
The melon for stating the complete female hua ' nan-type cucumber of white green is preferably 120～150g, most preferably more preferably 125～140g, 133.7g again；
The transverse diameter of the white green complete female hua ' nan-type cucumber is preferably 2.5～4.5cm, more preferably 3～4.0cm, most preferably
3.3cm。
The present invention is not particularly limited to the cultural method of the white green complete female hua ' nan-type cucumber, is preferably planted using ridge
Mode, 70～90cm of wide row space, little 30～50cm of line-spacing, 20～40cm of spacing in the rows, planting density is 3500～4000 plants/acre；More
Preferably 75～85cm of wide row space, little 35～45cm of line-spacing, 22～35cm of spacing in the rows, planting density is 3600～3900 plants/acre；Most
Preferably wide row space 80cm, little line-spacing 40cm, 25～30cm of spacing in the rows, planting density is 3700～3800 plants/acre.
When being cultivated using large-scale attached-greenhouse, planting density is preferably 1600～2000 plants/acre, more preferably 1700～
1900 plants/acre, most preferably 1800 plants/acre.
The selection of the complete female hua ' nan-type cucumber of a kind of white green provided the present invention with reference to embodiment is carried out in detail
Thin explanation, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
1) maternal seed selection：
Glue county purpleflower violet herb goes out nonrecurrent parent through 3 generations selfing isolation and selection, and the proterties of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 2, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn knurl is little and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 23cm of melon, melon transverse diameter 3cm.
Suizhong purpleflower violet herb obtains recurrent parent through 4 generations selfing isolation and selection, and the proterties of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white thorn knurl is little and rareness, and meat thickness accounts for the 65% of melon transverse diameter, the first female flower preferably it is raw on Section 11.4, melon is long
16cm, melon transverse diameter 3.4cm.
Nonrecurrent parent is returned with recurrent parent through continuous 5 generation, obtains backcross, eliminates non-full female plant, stays complete
Female plant, full female plant is processed through the silver nitrate solution that mass concentration is 0.03%, and induction produces male flower, and full female plant selfing is eliminated
The detached full female plant of self progeny's property spent, leaves the unseparated full female plant of colored property and is female parent.Maternal proterties is plant growing way
By force, leaf green, median size, in branch, in maturity, melon yellow-white, short cylinder shape, two ends are neat, and white thorn knurl is rare, medium big
Little, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw on Section 6.2, the long 18.4cm of melon, melon transverse diameter
3.3cm, melon weight 140g.
2) male parent seed selection：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening through 3 generations selfing point
Male parent is obtained from seed selection, the proterties of male parent raw in Section 10 for the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, white thorn knurl is few, in it is big, melon two ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 16.5cm, melon transverse diameter 3cm, melon weight 132g.
3) seed selection of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, the complete female South China type of white green is obtained yellow
Melon, proterties is complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, and melon surface is smooth without rib ditch, and white thorn knurl is few, median size,
Mouthfeel is tender and crisp, and plant strain growth gesture is strong, and melon is precocious, the long 18cm of melon, and the first female flower life in Section 3, and melon is 2.8cm, melon weight
133.7g, melon transverse diameter 3.3cm.
Embodiment 2
1) maternal seed selection：
Glue county purpleflower violet herb goes out nonrecurrent parent through 2 generations selfing isolation and selection, and the proterties of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 3, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn knurl is little and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 20cm of melon, melon transverse diameter 4cm.
Suizhong purpleflower violet herb obtains recurrent parent through 3 generations selfing isolation and selection, and the proterties of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white thorn knurl is little and rareness, and meat thickness accounts for the 60% of melon transverse diameter, the first female flower preferably it is raw in Section 10, melon is long
17cm, melon transverse diameter 3cm.
Nonrecurrent parent is returned with recurrent parent through continuous 6 generation, obtains backcross, and backcross is through mass concentration
Silver nitrate solution for 0.02% is processed, and superseded staminiferous plant leaves female plant, and female plant obtains female parent through selfing.Maternal proterties is
Plant growing way is strong, leaf green, median size, and in branch, in maturity, melon yellow-white, short cylinder shape, two ends are neat, white thorn knurl rareness,
Median size, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw in Section 5, the long 16cm of melon, melon transverse diameter
3cm, melon weight 150g.
2) male parent seed selection：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening through 3 generations selfing point
Male parent is obtained from seed selection, the proterties of male parent raw in Section 9 for the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, white thorn knurl is few, in it is big, melon two ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 16cm, melon transverse diameter 2.5cm, melon weight 125g.
3) seed selection of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, the complete female South China type of white green is obtained yellow
Melon, proterties is complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, and melon surface is smooth without rib ditch, and white thorn knurl is few, median size,
Mouthfeel is tender and crisp, and plant strain growth gesture is strong, and melon is precocious, the long 19cm of melon, and the first female flower life in Section 3, and melon is 2.5cm, melon weight
140g, melon transverse diameter 3cm.
Embodiment 3
1) maternal seed selection：
Glue county purpleflower violet herb goes out nonrecurrent parent through 4 generations selfing isolation and selection, and the proterties of nonrecurrent parent is mid-early maturity, the
One female flower life in Section 3, and growth potential is medium, medium blade size, sugar-preserved gourd bottle green, and white thorn knurl is little and rare, thickness is equal
It is even, in flavor quality on, bacteria resistance angular leaf spot, powdery mildew and downy mildew, the long 24cm of melon, melon transverse diameter 2.5cm.
Suizhong purpleflower violet herb obtains recurrent parent through 3 generations selfing isolation and selection, and the proterties of recurrent parent is late-maturing, and melon is yellowish-white
Color, melon tip circle, white thorn knurl is little and rareness, and meat thickness accounts for the 70% of melon transverse diameter, the first female flower preferably it is raw in Section 12, melon is long
18cm, melon transverse diameter 4.5cm.
Nonrecurrent parent is returned with recurrent parent through continuous 6 generation, obtains backcross, and backcross is through mass concentration
Silver nitrate solution for 0.04% is processed, and superseded staminiferous plant leaves female plant, and female plant obtains female parent through selfing.Maternal proterties is
Plant growing way is strong, leaf green, median size, and in branch, in maturity, melon yellow-white, short cylinder shape, two ends are neat, white thorn knurl rareness,
Median size, downy mildew resistance, anthracnose and powdery mildew, the first maternal female flower raw in Section 7, the long 17.5cm of melon, melon horizontal stroke
Footpath 2.5cm, melon weight 145g.
2) male parent seed selection：By the local dry land cucumber kind white jade spring (II) introduced from Northeast gardening through 3 generations selfing point
Male parent is obtained from seed selection, the proterties of male parent raw in Section 9 for the first female flower, and plant strain growth gesture is strong and tolerance to low temperature and weak light, leaf
Color is shallow, in branch, melon yellow green, white thorn knurl is few, in it is big, melon two ends are neat, and melon top is blunt, downy mildew resistance, anthracnose and powdery mildew, melon
Long 17cm, melon transverse diameter 3.5cm, melon weight 140g.
3) seed selection of the complete female hua ' nan-type cucumber of white green：By maternal and paternal hybrid, the complete female South China type of white green is obtained yellow
Melon, proterties is complete female, and chambers of the heart transverse diameter ratio is less than 0.5, and melon is green in vain, and melon surface is smooth without rib ditch, and white thorn knurl is few, median size,
Mouthfeel is tender and crisp, and plant strain growth gesture is strong, and melon is precocious, the long 19cm of melon, and the first female flower and given birth in Section 3, and melon weighs 135g 3cm, melon,
Melon transverse diameter 3.5cm.
Embodiment 4
The complete female hua ' nan-type cucumber of white green obtained to embodiment 1～3 is colonized, and site preparation is carried out before field planting, and per mu is applied
Decomposed manure 8000kg, calcium superphosphate 50kg, N, P, K composite fertilizer 50kg is planted, wide row space 80cm, little line-spacing 40cm using ridge,
Spacing in the rows 30cm, planting density is 3500 plants/acre.Routine Management management by methods is adopted after field planting, with the emerald green and close thorn kind in Xintai City
For control, 1 is the results are shown in Table：
The yield of the white green complete female hua ' nan-type cucumber of 1 embodiment of table 1～3
As can be drawn from Table 1, the complete female hua ' nan-type cucumber of white green that the present invention is cultivated than emerald green volume increase 26.3～
27.1%, thorn volume increase 22.6～23.5% closeer than Xintai City.
The identification of Jing Institute of Vegetables, Chinese Academy of Agricultural Sciences, the anti-yellowing melon of white green complete female hua ' nan-type cucumber that the present invention is cultivated
Powdery mildew disease refers to as 49.68, anti-grade in belonging to；Anti- cucumber downy mildew disease refers to as 31.30, belongs to disease-resistant grade；Anti-yellowing melon is withered
Disease of withering disease refers to as 34.58, anti-grade in belonging to；Anti- Cucumber Target Leaf Spot disease refers to as 20.50, belongs to disease-resistant grade.
Can be drawn by above example, the complete female hua ' nan-type cucumber of white green that the present invention is cultivated than emerald green volume increase 26.3～
27.1%, it is closeer than Xintai City thorn volume increase 22.6～23.5%, and in anti-powdery mildew of cucumber, anti-cucumber downy mildew, in anti-yellowing melon it is withered
Disease of withering and anti-Cucumber Target Leaf Spot.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of selection of the complete female hua ' nan-type cucumber of white green, comprises the following steps：
1) the female series kind obtained through selfing isolation and selection with purpleflower violet herb kind is as nonrecurrent parent, with purpleflower violet herb kind Jing
It is recurrent parent to cross the synoecy kind that selfing isolation and selection obtains, and the nonrecurrent parent and recurrent parent is continuous
In 4～6 generations, were returned, and obtained backcross, and the backcross is handed over through selfing or sisters, obtains female parent；
2) dry land cucumber kind into the raw kind on the 9th～13 section through the first female flower that 2～4 generations selfing isolation and selection is obtained
For male parent；
3) by the step 1) female parent that obtains and step 2) paternal hybrid that obtains, obtain the complete female hua ' nan-type cucumber of white green；
The step 1) and step 2) limit without time sequencing.
2. selection according to claim 1, it is characterised in that the first maternal female flower raw in the 5th～7 section
Upper, melon 16～20cm of length, melon 2.5～4cm of transverse diameter, in vain thorn knurl, melon weigh 120～160g, downy mildew resistance, anthracnose and powdery mildew.
3. selection according to claim 1, it is characterised in that the first female flower of the male parent raw the 9th～13
On section, melon 14～18cm of length, melon 2.5～4.5cm of transverse diameter pierce in vain knurl, and melon weighs 120～140g, downy mildew resistance, anthracnose and white powder
Disease.
4. selection according to claim 1, it is characterised in that the step 1) in nonrecurrent parent it is first female
Spend raw on the 2nd～3 section, melon 20～25cm of length, melon 2～4cm of transverse diameter pierce in vain knurl, bacteria resistance angular leaf spot, powdery mildew and frost
Mildew.
5. selection according to claim 1, it is characterised in that the step 2) in dry land cucumber kind be dry land cucumber
The kind white jade spring.
6. the selection according to claim 1 or 3, it is characterised in that the step 1) in recurrent parent described in seed selection
When selfing algebraically be 3～5 generations.
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| CN110278840A (en) * | 2019-08-08 | 2019-09-27 | 山东省农业科学院蔬菜花卉研究所 | A kind of secondary breeding method of cucumber |
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| CN101861827A (en) * | 2010-01-12 | 2010-10-20 | 南京农业大学 | Cultivation and application of full female processing cucumber Youjia full female 09 |
| CN102428865A (en) * | 2010-09-29 | 2012-05-02 | 深圳市农科蔬菜科技有限公司 | Breeding method for preparing European greenhouse-type cucumber all-female hybrid by utilizing female lines |
| CN103875523A (en) * | 2013-03-20 | 2014-06-25 | 山东省农业科学院蔬菜研究所 | Breeding method of cold-resistant all-female north China type cucumbers |
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| CN101861827A (en) * | 2010-01-12 | 2010-10-20 | 南京农业大学 | Cultivation and application of full female processing cucumber Youjia full female 09 |
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| CN110278840A (en) * | 2019-08-08 | 2019-09-27 | 山东省农业科学院蔬菜花卉研究所 | A kind of secondary breeding method of cucumber |
| CN110278840B (en) * | 2019-08-08 | 2021-04-23 | 山东省农业科学院蔬菜花卉研究所 | Secondary seed reproduction method for cucumbers |
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