CN103875523A - Breeding method of cold-resistant all-female north China type cucumbers - Google Patents
Breeding method of cold-resistant all-female north China type cucumbers Download PDFInfo
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- CN103875523A CN103875523A CN201410095808.2A CN201410095808A CN103875523A CN 103875523 A CN103875523 A CN 103875523A CN 201410095808 A CN201410095808 A CN 201410095808A CN 103875523 A CN103875523 A CN 103875523A
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Abstract
The invention relates to a breeding method of cold-resistant all-female north China type cucumbers in the technical field of the breeding method. The breeding method comprises the steps of carrying out hybridization on Xintaimici high-generation inbred line X8 and brilliant century No.2 high-generation inbred line M16, carrying out six-generation saturated backcross by utilizing the X8 as recurrent parent, and carrying out six-generation selfing purification to obtain female parent; carrying out hybridization by taking Tianjin excellent No. 10 high-generation inbred line as male parent to obtain the cold-resistant all-female north China type cucumbers. The obtained planets are in all-female type, high in cold resistance and capable of resisting brown patch, powdery mildew and wilt, thus being suitable for being planted in a sunlight greenhouse through the winter in north China.
Description
technical field
The present invention relates to breeding method technical field.Be particularly related to the selection of a kind of cold-resistant complete female North-China Type cucumber.
background technology
Cucumber is one of important vegetable crop of China, and the facility cultivation of surviving the winter is its important production model.North-China Type kind accounts for leadingly surviving the winter in facility cultivation, these crops for rotation are because cucumber at seedling stage is in high temperature long-day condition, and Female flower differentiation is subject to the impact of warm optical condition, often shows as female flower joint position high, and female-flower rate is low, affects especially early yield of its output.North-China Type lacks female germplasm in essence, causes North-China Type female series kind to lack.For head it off, in production, often adopt the method for external source plant growth regulator to increase melon processing, how much in close relations because of the application concentration of medicament and temperature, the growth period of seedling, usage amount, result of use is often difficult to guarantee, often occurs that Female flower differentiation is excessive, internode cripetura, growing point dwindles, the situation such as pinch serious, and result causes cucumber to nourish and grow and reproductive growth is unbalance, plant concentrations result, the situations such as knot melon is long interval time, and early ageing is serious occur.Utilizing female series kind is the effective measures that address the above problem.Female series kind cucumber plant is only opened female flower and is not opened male flower, and Female flower differentiation is not affected by environment, and plant strain growth stalwartness.The most American-European fruit type kinds for introducing of complete female kind in domestic production at present, this veriety fruit is short and small smooth poor without knurl and cold resistance, and wintering habit is poor, is not suitable for the consumption habit in the close thorn of North-China Type cucumber producing region, application limitation is very large, can not meet need of production.Therefore the cold-resistant gynoecy North China of a seed selection close thorn type kind solution production difficult problem is necessary and urgent.
summary of the invention
For above-mentioned cucumber the survive the winter production status of facility cultivation and an urgent demand to cold-resistant variety, the invention provides a kind of selection of cold-resistant complete female North-China Type cucumber variety, the kind of seed selection can better adapt to produce, greatly enrich China's cucumber ecotype, reduce peasant household and lure using of female medicine and issuable harm seedling stage, peasant is increased production and improved productivity effective assurance is provided.
The present invention is achieved by the following measures:
A selection for cold-resistant complete female North-China Type cucumber, comprises the following steps:
1, maternal seed selection:
1.1 by outstanding China's North-China Type landrace Xintai City close thorn kind inbreeding of more generation, get Gao Dai inbred line X8;
Recurrent parent X8:
This is the purifying strain being gone out by the outstanding landrace of China's North-China Type Xintai City directed selfed breeding of 8 generations of close thorn.X8 is monoecism, and sugar-preserved gourd color of the leather is even, green, and without yellow cord, chilling under low light is strong, anti-blight, brown spot, powdery mildew.
The 1.2 South China type female cucumber kinds of introducing from Japan: No. two (commercially available kinds of century splendidness, on the books in the article " " brilliant No. two of century " Cucumber High-yield Culture technology " of " vegetables " the 9th interim Du's Yulin in 2002) inbreeding of more generation, after each selfing, choose the plant insensitive to warm light, the first female flower joint position 2-3 joint of female, female sex expression entirely and reserve seed for planting, get Gao Dai inbred line M16;
Female source M16:
The female material of the South China type 6 generation selfed breeding of being introduced by Japan goes out to stablize pure and mild female series M16.M16 is for entirely female, and female sex expression is insensitive to warm light.
1.3 M16 and X8 hybridization forms F1, the F1 of acquisition and recurrent parent X8 is carried out to 6 generations saturated backcrossing and obtain BC6, and BC1-BC6 selected in six generations, and plant female, that sting close, the long sugar-preserved gourd of knurl (more than sugar-preserved gourd length 30cm) backcrosses;
BC6 is carried out 6 generations selfing purifying by 1.4, in conjunction with indoor and field cold resistance, Disease Resistance Identification, chooses female, cold-resistant, mildew-resistance, anti-brown spot, the good female series of anti-blight entirely and reserve seed for planting, must MC2065 after 6 generations selfing purifying, as female parent;
MC2065 is characterized as entirely female, and female stable, sugar-preserved gourd color of the leather is green, evenly, without yellow cord, close thorn, weak light resistance is outstanding, mildew-resistance, anti-brown spot, anti-blight.
2, male parent seed selection:
From introducing a fine variety material Jinyou No.10 (commercially available kind, breathe out pure as jade grade mentions in interim article of " China's Vegetable " calendar year 2001 the 5th " seed selection of spring plastic tent cucumber new varieties Jinyou No.10 ") in the monoecism of seed selection, sugar-preserved gourd color of the leather is green, evenly, without yellow cord, chilling under low light is strong, the good inbred line of mildew-resistance, anti-brown spot, called after YQ217-1-1.
3, hybridization:
By maternal MC2065 and male parent YQ217-1-1 hybridization, to obtain final product.
" MC2065*YQ217-1-1 " hybrid combination is through 3 years varietal yield tests, 1 year regional test and the test of annual product, show entirely female, strong adaptability, strong stress resistance, names as Shandong vegetables 869, this kind is at 10 ℃ of left and right low temperature, 140 μ mol/m
2under s low light condition, vine growth and development has no significant effect, not shrinkage of blade, and growing point growth is normal, shows strong cold resistance.
Beneficial effect of the present invention:
The Shandong vegetables 869 that adopt the inventive method to cultivate, plant allfemale type, sugar-preserved gourd color of the leather is even, green, without yellow cord, anti-brown spot, powdery mildew, fusarium wilt, female stable, long season cultivation output 10000kg/667m
2above; The survive the winter greenhouse by solar heat long season cultivation of applicable North China.
Embodiment
Below in conjunction with specific embodiment, selection of the present invention is further described.
One, the selection of cold-resistant complete female North-China Type cucumber Shandong vegetables 869
1, maternal seed selection:
The target of maternal seed selection: entirely female, cold resistance is strong, disease-resistant.
1.1 with the close thorn 8 generation selfing in domestic good landrace Xintai City, get Gao Dai inbred line X8.
1.2 on a large amount of bases of collecting domestic and international female cucumber variety source, by selfing directional separation and female, disease-resistant, tolerance to low temperature and weak light evaluation, from having obtained the good female material M16 of South China type that complete female, female sex expression is insensitive to warm light, the first female flower saves position 2-3 joint one female germplasm century of Japan brilliant No. two (commercially available kinds, on the books in the article " " brilliant No. two of century " Cucumber High-yield Culture technology " of " vegetables " the 9th interim Du's Yulin in 2002) after the selfing of 6 generations.
1.3 take M16 as maternal, and X8 is that male parent hybridize and obtained F1, and F1 performance is entirely female, and sugar-preserved gourd proterties, between Parent, backcrosses with X8, acquisition BC1; Repeat said process, continue to select individual plant female, thorn close, the long sugar-preserved gourd of knurl (more than 30cm) to backcross, to backcrossing, in 6 generations, obtained BC6.
1.4 utilize and lure seedling stage male technological guide BC6 female strain to produce male flower to carry out individual plant selfing, the concrete male technology that lures is: seedling carries out shading spraying processing after 17:00 in the afternoon at the liquor argenti nitratis ophthalmicus of 4 leaf 1 heart 300mg/L, the spray of leave dual sides and growing point is even to be covered with little water droplet and not to flow down and be advisable, after spray, carry out immediately full dark processing 12 hours, processed once again every 2 days.Select the close thorn type of female, long melon (more than 30cm) plant to reserve seed for planting.
Growth mid-term and later stage are carried out the screening of disease resistance field test, the strain seed that meets breeding objective is divided into 3 parts, portion carry out seedling stage disease-resistant inoculated identification and tolerance to low temperature and weak light identify, tolerance to low temperature and weak light, disease resistance, yielding ability, the sugar-preserved gourd commodity property in field are observed in survive the winter Cultivation in Greenhouse plantation of portion, in conjunction with the artificial qualification result in field and laboratory, the strain that meets breeding objective is proceeded to selfing.Repeat above process, selfing purifying screened after 6 generations, formulate out entirely female, sugar-preserved gourd color of the leather is green, evenly, without yellow cord, close thorn, weak light resistance is outstanding, the good female series MC2065 of North-China Type of mildew-resistance, anti-brown spot, anti-blight.
2, the seed selection of male parent:
The breeding goal of male parent: plant type compactness, fruit is good, disease-resistant, chilling under low light is strong.
Male parent YQ217-1-1 is from introducing a fine variety material Jinyou No.10 (commercially available kind, breathe out pure as jade grade mentions in interim article of " China's Vegetable " calendar year 2001 the 5th " seed selection of spring plastic tent cucumber new varieties Jinyou No.10 ") in the monoecism of seed selection, color of the leather is even, green, without yellow cord, chilling under low light is strong, the good inbred line of mildew-resistance, anti-brown spot.
3, hybridization
Maternal MC2065 and male parent YQ217-1-1 hybridization, to obtain final product.
" MC2065*YQ217-1-1 " hybrid combination is through 3 years varietal yield tests, 1 year regional test and the test of annual product, show entirely female, long melon, strong adaptability, strong stress resistance, names as Shandong vegetables 869, this kind is at 10 ℃ of left and right low temperature, 140 μ mol/m
2under s low light condition, vine growth and development has no significant effect, not shrinkage of blade, and growing point growth is normal, shows strong cold resistance.
Two, the feature of Shandong vegetables 869:
(1) entirely female: plant is entirely female, female stable.(2) cold resistance is good: the stubble plantation cold resistance of surviving the winter is strong, is difficult for female flower and pinches, and returns head spring fast, the seedling of not having a rest.(3) yielding ability is good: plant strain growth gesture is strong.(4) color of the leather is even, green, and without yellow cord, long melon, stings close.(5) disease resistance is strong: anti-brown spot, powdery mildew, fusarium wilt.
Three, high-yield culture technique main points:
Day-light greenhouse overwintering cultivation is generally in sowing mid-September, Cai Yong ?the mode of seed pumpkin graft seedling growth, field planting when physiology seedling age reaches 2-3 leaf 1 heart.Produce and adopt high bedding cultivation mode, before field planting, use sufficient base fertilizer.Plantation density is every 667 ㎡ 3600 strain left and right, after field planting, should not too restrain the growth of seedlings, and root melon carries out rich water supply after sitting.This kind melon code is close, need not spray the plant growth regulator such as Zenggualing.Low temperature successive cloudy days should increase illumination as far as possible, keep the normal photosynthesis of cucumber.February next year, should pluck in time melon, can not cross dividing potential drop melon, strengthen rich water supply simultaneously.Damage by disease and insect is relied mainly on prevention, attention control downy mildew and brown spot in earlier stage, later stage attention control trialeurodes vaporariorum and aphid.Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not subject to the restriction of embodiment; other is any does not deviate from change, modification, the combination made under Spirit Essence of the present invention and principle, substitute, simplify and all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (3)
1. a selection for cold-resistant complete female North-China Type cucumber, is characterized in that comprising the following steps:
(1) maternal seed selection:
1.1 by close Xintai City thorn kind inbreeding of more generation, get Gao Dai inbred line X8;
1.2 No. two inbreeding of more generation of the female material of South China type century splendidness of introducing from Japan, get Gao Dai inbred line M16;
1.3 M16 and X8 hybridization forms F1, the F1 of acquisition and recurrent parent X8 is carried out to 6 generations saturated backcrossing and obtain BC6, and the middle selection that at every turn backcrosses is female, sting close, the more than sugar-preserved gourd length 30cm plant of knurl backcrosses;
BC6 is carried out 6 generations selfing purifying by 1.4, each selfing choose entirely female, sugar-preserved gourd color of the leather is green, evenly, without yellow cord, close thorn, weak light resistance is outstanding, the female series of mildew-resistance, anti-brown spot, anti-blight is reserved seed for planting, and obtains MC2065, as female parent after 6 generations selfing purifying;
(2) male parent seed selection:
From Jinyou No.10 self progeny, select monoecism, sugar-preserved gourd color of the leather is green, and evenly, without yellow cord, chilling under low light is strong, the inbred line of mildew-resistance, anti-brown spot, and called after YQ217-1-1, as male parent;
(3) hybridization:
By maternal MC2065 and male parent YQ217-1-1 hybridization, to obtain final product.
2. selection according to claim 1, is characterized in that in step 1.1, Xintai City obtains X8 in 8 generations of close thorn selfing.
3. selection according to claim 1, is characterized in that obtaining M16 with brilliant No. two 6 generations of selfing in the female material of the South China type century of introducing from Japan in step 1.2.
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| CN 201310088888 CN103120120A (en) | 2013-03-20 | 2013-03-20 | Method for breeding cold-resistant all-female north China cucumber |
| CN201410095808.2A CN103875523A (en) | 2013-03-20 | 2014-03-17 | Breeding method of cold-resistant all-female north China type cucumbers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134983A (en) * | 2016-07-26 | 2016-11-23 | 山东省农业科学院蔬菜花卉研究所 | A kind of breeding method of glossy type cucumber female series |
| CN106613917A (en) * | 2017-01-12 | 2017-05-10 | 青岛市农业科学研究院 | Breeding method for white green all-female Huanan-type cucumber |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120120A (en) * | 2013-03-20 | 2013-05-29 | 山东省农业科学院蔬菜研究所 | Method for breeding cold-resistant all-female north China cucumber |
| CN106134984A (en) * | 2016-08-30 | 2016-11-23 | 郑州市蔬菜研究所 | A kind of method of east show Fructus Cucumidis sativi cross-breeding |
| CN108703065B (en) * | 2018-05-29 | 2021-06-08 | 唐山市农业科学研究院 | Breeding method of no-female flowers or few-female flowers of cucumber plant with less than 8 knots |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103120120A (en) * | 2013-03-20 | 2013-05-29 | 山东省农业科学院蔬菜研究所 | Method for breeding cold-resistant all-female north China cucumber |
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| CN103120120A (en) * | 2013-03-20 | 2013-05-29 | 山东省农业科学院蔬菜研究所 | Method for breeding cold-resistant all-female north China cucumber |
Non-Patent Citations (2)
| Title |
|---|
| 孙小镭，等: "华北型黄瓜雌性系核心种质MC2065 的创制", 《山东农业科学》 * |
| 李建吾，等: "保护地黄瓜新品种‘旺世’", 《园艺学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134983A (en) * | 2016-07-26 | 2016-11-23 | 山东省农业科学院蔬菜花卉研究所 | A kind of breeding method of glossy type cucumber female series |
| CN106613917A (en) * | 2017-01-12 | 2017-05-10 | 青岛市农业科学研究院 | Breeding method for white green all-female Huanan-type cucumber |
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Address after: 250010 No. 202 industrial North Road, Licheng District, Shandong, Ji'nan Applicant after: Vegetable or flower institute of Agriculture in Shandong Province academy of sciences Address before: 250010 No. 202 industrial North Road, Licheng District, Shandong, Ji'nan Applicant before: Vegetable Research Institute, Shandong Academy of Agricultural Sciences |
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Application publication date: 20140625 |