CN108901831B - Breeding method of indica rice restorer line with strong rice blast resistance and strong superiority - Google Patents

Breeding method of indica rice restorer line with strong rice blast resistance and strong superiority Download PDF

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CN108901831B
CN108901831B CN201810941763.4A CN201810941763A CN108901831B CN 108901831 B CN108901831 B CN 108901831B CN 201810941763 A CN201810941763 A CN 201810941763A CN 108901831 B CN108901831 B CN 108901831B
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rice
rice blast
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indica
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CN108901831A (en
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李育红
李爱宏
吴云雨
肖宁
戴正元
刘广青
潘存红
周长海
余玲
黄年生
张小祥
季红娟
王闯
王志平
吴诗清
李响
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JIANGSU LIXIAHE REGION AGRICULTURAL RESEARCH INSTITUTE
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JIANGSU LIXIAHE REGION AGRICULTURAL RESEARCH INSTITUTE
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract

The invention relates to a breeding method of a strong-superiority indica rice restorer line capable of resisting rice blast, belonging to the technical field of crop genetic breeding. The method relates to a method for breeding a new rice blast resistant strong dominant restoring line by taking a first indica rice restoring line parent (Huazhan) with rice blast resistance, excellent comprehensive agronomic characters, strong superiority and good combining ability as a rice blast resistant gene donor parent, taking a second indica rice restoring line parent (Huazhan) which is not resistant to rice blast, excellent comprehensive agronomic characters, strong superiority and good combining ability as a receptor parent, hybridizing, taking the first parent (Huazhan) as a recurrent parent, backcrossing once, detecting rice blast resistant genes such as Pi2, Pi54, Pid3, Pita and Pib contained in the first indica rice restoring line (Huazhan) by using molecular markers, breeding a new rice blast resistant strong dominant restoring line by synthesizing agronomic characters and rice blast resistance identification results, and further pairing with different sterile lines to breed a rice blast resistant high-quality hybrid new rice blast resistant rice combination, thereby improving the rice blast resistance of the first hybrid generation and meeting the requirements of current rice production.

Description

Breeding method of indica rice restorer line with strong rice blast resistance and strong superiority
Technical Field
The invention provides a breeding method of a strong rice blast resistance advantage indica rice restorer, belonging to the technical field of crop genetic breeding.
Background
Rice is one of the main grain crops in China and even the world, and high yield, high quality and disease resistance are important breeding targets of the rice. The hybrid rice is widely applied and popularized as one of the main ways for improving the yield of the rice, makes great contribution to the safe production of grains, but limits the application in the production due to the fact that the popularized variety does not resist rice blast or resistance degeneration in the near future. Therefore, there is an urgent need to improve the rice blast resistance of hybrid rice and to meet the demand for rice production. The rice blast caused by the Magnaporthe oryzae is one of the most main fungal diseases threatening the safe production of rice in the world, and the yield reduction of rice caused by the rice blast reaches 10 to 30 percent every year in the world. In recent 40 years, rice blast has become a main problem in hybrid rice production due to narrow genetic basis of parents and diversity of highly pathogenic rice blast germs, and in recent years, rice blast continuously and greatly outbreaks occur in rice growing areas such as southwest, middle and downstream of Yangtze river, northeast and the like, and further great hidden danger is brought to the safe production of rice in China. The method for preventing and treating the diseases is the most economic and effective method by utilizing the resistance genes and cultivating new disease-resistant hybrid indica rice combinations. The advantage restorer line is an important germplasm basis for the hybrid indica rice combination breeding, enhances the breeding of the strong rice blast resistance advantage restorer line, further prepares a new rice blast resistance hybrid indica rice combination with high quality and high yield, and is one of the main ways for reducing the harm of rice blast.
Genetic analysis, gene mapping and cloning related researches related to rice blast resistance are carried out more. Since 3 independently inherited rice blast resistance genes, Pia, Pii and Pik, were first reported by Yamasaki and Kiyosawa (1966) in the last 60 th century, at least 88 major resistance genes of 73 sites were reported up to now, 27 of which were cloned (Chinese national Rice Gene database, http:// www.ricedata.cn/gene; Ma et). Huazhan is used as an indica rice restorer line (national rice data center, http:// www.ricedata.cn), is widely applied to hybrid indica rice breeding in recent years, a configured hybrid combination shows good resistance to rice blast, Huazhan also shows broad-spectrum resistance to rice blast, molecular marker positioning positions disease-resistant genes contained in Huazhan to a Pi2/Pi9/Pi50 gene region on a 6 th chromosome of rice, and the Pi2 allele sequence analysis proves that the Huazhan contains a Pi2 gene. According to the information of the broad-spectrum rice blast resistance genes which are positioned or cloned, the research team designs molecular markers, detects that Huazhan simultaneously contains the rice blast resistance genes such as Pi54, Pid3, Pita and Pib, and the like, and proves that the Huazhan shows broad-spectrum resistance to rice blast from another aspect. In modern breeding, molecular Marker Assisted Selection (MAS) has become a main approach for breeding disease-resistant varieties, which can increase the reliability of selection and reduce the breeding workload and the breeding cost.
The invention content is as follows:
the invention aims to provide a breeding method of a strong rice blast resistance advantage indica rice restorer line.
Another purpose of the invention is to provide a breeding method of a new rice blast-resistant two-line hybrid indica rice combination with high quality and high yield.
The purpose of the invention can be realized by the following technical scheme:
a breeding method of a strong rice blast resistance advantage indica rice restorer line comprises the following steps:
1) the first filial generation F is planted by taking the indica type rice restorer line (parent I) with rice blast resistance, excellent comprehensive agronomic characters, strong superiority and good combining ability as a female parent and taking the indica type rice restorer line (parent II) with excellent comprehensive agronomic characters, strong superiority and good combining ability as a male parent for hybridization1Then taking the parent as the recurrent parent, backcrossing once, and harvesting the BC1F1Seeds;
2) planting BC1F1Population, detection of BC by molecular markers1F1The rice blast resistance gene contained in a parent in a group is simultaneously inspected for main agronomic characters, a single plant which has the main agronomic characters superior to recurrent parents and contains the rice blast resistance gene is selected and mixed to obtain BC1F2Seeds;
3) big group planting BC1F2Planting 500 plants, detecting the rice blast resistance gene contained in a parent through molecular markers, investigating agronomic characters, integrating the molecular marker detection result and the agronomic character selection, harvesting single plant seeds, and establishing a plant line BC1F3；
4) Planting BC by strains1F3Using parent as reference, integrating MAS detection result, agronomic character selection and pedigree breeding, harvesting single plant seed, continuously establishing strain BC1F4；
5) Carrying out molecular marker detection on rice blast resistance genes contained in the first parent generation by generation, selecting a single plant with main agronomic characters superior to the first parent generation, and repeating the step 4) until BC is reached1F7Selecting a strain line containing rice blast resistance genes and having main agronomic characters superior to those of the parent I in the generation, carrying out test cross on the strain line by using pearl indica 97A, and harvesting BC1F8Generation seeds and corresponding test cross seeds;
6)BC1F8planting the lines in pairs and testing the hybrid, identifying heterosis, and respectively stabilizing BC1F8Carrying out resistance identification on the strains of the seedling blast and the panicle blast; and (3) synthesizing the heterosis, the main agronomic characters and the rice blast resistance identification result, selecting a stable strain line meeting the breeding target, and breeding into a new rice blast resistant indica rice restorer line with strong dominance.
Wherein, the parent is preferably Huazhan; parent II is preferably radiata recovery 838.
In the steps 2), 3), 4), 5), the rice blast resistance genes Pi2, Pi54, Pid3, Pita and Pib contained in the parents are preferably detected by using molecular markers.
Preferably, the step 2), the step 3), the step 4) and the step 5), the agronomic character selection is started from the early generation, and only the individual plants with the agronomic characters superior to those of the first parent can be selected.
Preferably, collecting rice ears naturally infected with rice blast from rice blast retransmission disease area in step 6), culturing pathogenic bacteria, selecting representative strain, expanding culture, eluting rice blast spore, and adjusting concentration to 2 × 105each.mL-1To doInoculum for identifying rice blast resistance.
The breeding method preferably further comprises the following steps:
7) expanding and propagating new rice blast resistant strong dominant indica type rice restorer line seeds, simultaneously, taking the indica type rice restorer line seeds as a male parent and taking different sterile lines as female parents to configure a hybridization combination, identifying the grouping superiority of the bred rice blast resistant strong dominant restorer line, inoculating rice blast germs used in the step 6) at the booting stage, identifying field resistance in different rice blast disease gardens, synthesizing the identification results of panicle blast and disease garden resistance, and comprehensively evaluating the rice blast resistance of the hybridization combination which is bred into the restorer line and configured by taking the restorer line as the male parent.
The indica rice restorer line Yanghui 1512 with strong rice blast resistance and strong superiority bred according to the method has the characteristic characteristics that: the rice blast resistant indica rice restoring line belongs to an indica type medium indica rice restoring line, has the advantages of 130 days of the whole growth period, 130.6cm of plant height, 26.1cm of ear length, 15.12 thousand of effective ears per mu, 171.9 total grains per ear, 93.42 percent of seed setting rate, 21.8 grams of thousand grain weight, 1.4 of rice blast comprehensive index, rice blast resistance and strong matching advantages: the combination of Yanghui 1512 and different sterile lines has obvious yield advantage, all combinations are increased in yield compared with the control Fengliouyou No. four, and the rice blast resistance is achieved.
The breeding method of the new rice blast-resistant high-quality high-yield two-line hybrid indica rice combination comprises the following steps:
on the basis of breeding a restoring line with strong rice blast resistance and strong superiority, different sterile lines are used as female parents to configure a hybrid combination, the panicle blast resistance of the hybrid combination is identified on the premise of ensuring that the yield of the hybrid progeny is higher and the yield of the hybrid progeny is increased by No. 4 (indica control variety in the national regional test), and the rice blast resistance of the configured hybrid combination is comprehensively evaluated by combining the resistance identification results of different rice blast disease gardens; and harvesting the rice of hybrid progeny of all the hybrid combinations, and detecting the rice quality of all the hybrid combinations according to the national standard of high-quality rice. Finally, the new rice blast-resistant hybrid indica rice combination with high quality and high yield is obtained.
Wherein the rice blast resistance of the hybrid combination is preferably identified using the rice blast strain used in step 6).
The bred restoring line with strong rice blast resistance and strong superiority is Yanghui 1512 preferably.
The different rice blast disease gardens are preferably selected from 4 rice blast disease gardens of Hangzhou in Fujian, Jinggang mountain in Jiangxi, Jinzhai in Anhui and Enshi in Hubei.
According to the method, the new hybrid indica rice combination Yangliou 512 and Quangliou 1512 with rice blast resistance and high quality and high yield are bred by taking the strong rice blast resistance advantage indica rice restorer Yanghui 1512 as a male parent and taking Yangliang 5S and Quanluo 9311A as female parents;
the Yangliangyou 512 is characterized in that: belongs to indica type two-line hybrid rice varieties, is planted in middle and lower reaches of Yangtze river as middle rice in one season, has the full growth period of 136.1 days, is 0.4 days later than the Harvest-Shuangyou-four (CK), has the average plant height of 122.1 cm, has the effective spike number of 16.8 ten thousand per mu, has the spike length of 25.8 cm, has the total spike number of 217.9 grains per spike, has the maturing rate of 79.1 percent and has the thousand-grain weight of 23.5 grams; resistance: the rice blast comprehensive index is 3.6 grade, and the highest panicle blast loss rate is 5 grade; grade 7 of bacterial leaf blight; brown planthopper grade 7; the main indexes of the rice quality are as follows: the brown rice rate is 79.8%, the whole rice rate is 58.7%, the aspect ratio is 3.2, the chalky grain rate is 18.7%, the chalky degree is 3.0%, the transparency is 2 grade, the alkali digestion value is 3.4 grade, the gel consistency is 75mm, the amylose content is 13.4%, and the comprehensive rating is common in ministerial marks; the average yield per mu of a domestic district test in 2017 is 649.06 kg, the yield is increased by 7.17% compared with the yield of Fengshi you No. four (CK), and the yield point proportion is 92.9%;
quanyou 1512 is characterized in that: belongs to indica type three-line hybrid rice variety, is planted as middle rice in one season at middle and lower reaches of Yangtze river, has a full growth period of 133.7 days, and is precocious for 1.0 day compared with Fengshi excellent No. four (CK); the average plant height is 126.9 cm, the effective spike number per mu is 15.7 ten thousand ears, the spike length is 26.2 cm, the total number of the spikes is 192.0 grains, the seed setting rate is 83.6 percent, and the thousand grain weight is 27.3 g; resistance: the rice blast comprehensive index is 4.0 grade, and the highest panicle blast loss rate is 7 grade; grade 5 of bacterial leaf blight; brown planthopper grade 9; the heat resistance in the heading stage is grade 3; the main indexes of the rice quality are as follows: the whole rice rate is 59.3%, the length-width ratio is 3.2, the chalky grain rate is 26%, the chalky degree is 8.0%, the gel consistency is 86 mm, and the amylose content is 13.8%; in 2016, the rice is participated in green channel region tests of late ripening groups of middle and lower indica rice in the Yangtze river of the southern rice region of China, the average yield per mu is 665.04 kg, the yield is increased by 6.96 percent compared with the yield of Fengliou No. four (CK), and the level reaches extremely remarkable level; the average yield per mu of the 2017 continuing test is 631.41 kg, the yield is increased by 3.29 percent compared with the yield of Fengliangyou No. four (CK), and the extremely obvious level is achieved; the average yield per mu of a two-year district test is 648.23 kg, and the yield is increased by 4.99 percent compared with the yield of Fengliangyou No. four (CK); in a synchronous production test in 2017, the average yield per mu is 624.99 kg, and the yield is increased by 5.60 percent compared with the yield of Fengshi-you-No. four (CK), so that the level is extremely obvious.
The invention has the beneficial effects that:
the invention discloses a breeding method of a indica rice restorer line with strong rice blast resistance and strong superiority. The method relates to the detection of rice blast resistance genes contained in recurrent parent (parent I) by using molecular markers; in the lower generation (BC)1F1) Starting to select agronomic characters and expanding selection population (BC)1F2) And selecting a single plant with agronomic characters superior to those of a recurrent parent (parent I); advanced generation (BC)1F8) Inspecting and identifying heterosis and main agronomic characters of the stable strain, and carrying out field resistance identification on the stable strain through seedling blast, panicle blast and disease nursery; finally, combining the heterosis, the main agronomic characters and the rice blast resistance identification results to breed a new rice blast resistant strong-dominance indica rice restoring line; the restorer line bred by the method is matched with different sterile lines, and the new rice blast resistant hybrid rice combination with high quality and high yield is bred by carrying out yield advantage identification, rice quality detection, panicle blast inoculation identification and rice blast disease nursery field resistance identification on the hybrid combination. The hybrid combination has the following advantages: (1) the rice blast resistance contained in the recurrent parent (parent I) is detected by molecular markers and genetically improved, so that the rice blast resistance is expressed in a bred restorer line and hybrid rice combination, and the rice blast resistance is further realized, thereby being favorable for protecting the intellectual property of varieties. (2) The bred restoring line not only has the rice blast resistance basically same as that of a recurrent parent (parent I), but also has the agronomic characters superior to the rice blast resistance, and the hybrid combination configured by taking the restoring line as a male parent has large yield potential. (3) The new hybrid indica rice combination bred by matching different sterile lines as female parents with the newly bred restoring line with strong rice blast resistance and strong superiority not only resists rice blast, but also has high and stable yield.
Description of the drawings:
FIG. 1: the breeding pedigree of the indica rice restorer line with strong rice blast resistance and strong superiority is shown as a schematic diagram
The specific implementation mode is as follows:
the breeding method of the indica rice restorer line with strong rice blast resistance and strong superiority is further illustrated by combining the attached drawings.
Example 1:
1. breeding materials
1) Huazhan (parent I) is used as a female parent. Huazhan is a rice blast resistant indica rice restorer line cultivated by the Chinese rice institute. The variety has been applied and authorized to the new plant variety protection office of Ministry of agriculture, and the variety right number: CNA 20080059.0, available from Rice research institute of China. The variety is a backbone restoring line of hybrid rice in China, and the prepared hybrid rice has the advantages of high yield, high quality, rice blast resistance, wide adaptability and the like.
2) The radiata recovery 838 (parent II) is used as a male parent. Radiahui 838 is a conventional indica rice variety cultivated by the atomic energy institute of sichuan province. Although the variety does not apply new variety rights to new plant variety protection offices in Ministry of agriculture, the variety is widely applied as an indica rice restorer since being approved as an indica conventional rice variety in 1995, is generally existed in national agricultural research institutions, and can be purchased from Sichuan atomic energy research institute in a large amount. The hybrid rice is a backbone restoring line of hybrid indica rice in China, and the prepared hybrid rice has the advantages of high yield, wide adaptability and the like.
2. The breeding of indica rice restorer line with strong rice blast resistance is shown in figure 1:
1) the hybrid generation F is planted by hybridizing the hybrid generation taking indica rice restorer Huazhan as female parent with rice blast resistance, excellent comprehensive agronomic character, strong superiority and good combining ability and taking indica rice restorer radiata 838 as male parent with excellent comprehensive agronomic character, strong superiority and good combining ability1Then using Huazhan as recurrent parent to backcross for one generation and harvest BC1F1Seeds;
2) planting BC1F1Population 40, detection of BC by molecular marker1F1The Chinese population contains the Rice blast Resistance Genes Pi2, Pi54, Pid3, Pita and Pib (the primers used for marking are detailed in Yunyu Wu, Ning Xiao, Ling Yu, Cunhong Pan, Yuhong Li, Xiaoxing Zhang, Guangqing Liu, Zhengyuan Dai, Xuebiao Pan, Aihong Li.combination Patters of Major R Genes their levels of reaction to the M.organism in Rice (Oryza sativa L.). PLoS ONE,2015,10(6): e0126130), and the main agronomic properties are examined at the same timeSelecting 19 sterile single plants which have main agronomic characters superior to recurrent parents and contain rice blast resistance genes, and mixing and harvesting to obtain BC1F2Seeds;
3)BC1F2sowing seeds harvested in the first generation, planting 500 plants, detecting rice blast resistance genes Pi2, Pi54, Pid3, Pita and Pib through molecular markers, inspecting agronomic characters, integrating the molecular marker detection result and the agronomic character selection, selecting 30 single plants, and harvesting BC1F3Seeds and establishing a strain;
4) planting BC by plant lines1F3Using Huazhan as a contrast, integrating MAS detection results, investigating main agronomic characters and genealogical breeding, selecting 16 single plants, and harvesting BC1F4Seeds, and continuing to establish a strain;
5) carrying out molecular marker detection on rice blast resistant genes Pi2, Pi54, Pid3, Pita and Pib generation by generation, selecting single plants with main agronomic characters superior to Huazhan, and repeating the step 4) until BC1F7Selecting 5 strains which contain rice blast resistance genes and have main agronomic characters superior to Huazhan in the generation, and carrying out test cross by using Zhenxian 97A;
6)BC1F8planting 5 strains in pairs and testing cross species to identify heterosis, simultaneously collecting rice ears naturally infected with rice blast from rice blast retransmission disease areas such as Guangdong, Hubei, Hainan, Zhejiang and Jiangsu, culturing germs, selecting 5 representative virulent monospore strains GD18-3(B1, the pathogenic rate is 65.6%), ES9-5-1(B15, the pathogenic rate is 75.0%), hnn1-1(C1, the pathogenic rate is 59.4%), ZJ3-4(A15, the pathogenic rate is 63.3%) and XK8-4-4(G1, the pathogenic rate is 37.5%), eluting rice blast spores after expanding culture, adjusting the concentration to 2 x 105. mL-1, and respectively serving as inoculants for identification on stable BC1F8Carrying out resistance identification on the strains of the seedling blast and the panicle blast; and (3) synthesizing the heterosis, the main agronomic characters and the rice blast resistance identification result, selecting a stable line which meets the breeding target, and breeding a new rice blast resistant strong-dominance indica rice restorer line Yanghui 1512.
7) Propagating new rice blast resistant strong dominant indica rice restorer line Yanghui 1512 seeds in Hainan, simultaneously using the new rice blast resistant strong dominant indica rice restorer line as a male parent and using different sterile lines as female parents, configuring a hybridization combination, identifying the grouping advantages of the bred rice blast resistant strong dominant restorer line in the season, inoculating rice blast germs used in the step 6) in the booting stage, identifying field resistance in 4 rice blast disease gardens of Hangzhou, Jiangxi Jinggang mountain, Anhui Jinzhai and Hubei, synthesizing panicle blast and disease garden resistance identification results, and comprehensively evaluating the rice blast resistance of the bred restorer line and the hybridization combination configured by using the bred restorer line as the male parent.
The indica rice restorer line Yanghui 1512 with strong rice blast resistance and strong superiority bred according to the method has the following characteristic characteristics:
characteristic features: belongs to the restoring line of indica type. The total growth period is about 130 days, the plant height is 130.6cm, the ear length is 26.1cm, the effective ear per mu is 15.12 ten thousand, the total number of the ears per mu is 171.9, the seed setting rate is 93.42 percent, and the thousand-seed weight is 21.8 g. The comprehensive index of the rice blast is 1.4, and the rice blast is resistant.
The matching advantages are strong: the combination of Yanghui 1512 and different sterile lines has obvious yield advantage, all combinations are increased in yield compared with the control Fengliouyou No. four, and the rice blast resistance is achieved.
3. The new combination breeding of two-line hybrid rice with high quality and high yield and capable of resisting rice blast comprises the following steps:
1) the new hybrid indica rice combination Yangliangyou 512 and Quanyou 1512 resisting rice blast and having high quality and high yield are bred by taking Yanghui 1512 as a male parent and Yanghui 5S and Quanlin 9311A as a female parent. Performing advantage identification in Yangzhou, inoculating rice blast germs in the booting stage, wherein the panicle blast is 1.6 and 1.0 respectively, and the rice blast germs can be resistant to rice blast; the quality ratio test is continuously carried out for two years, and the perennial reissued areas of rice blast such as Hung-Shi, Jiangxi Tuchuan, Anhui Jinzhai, Hangzhou in Fujian and the like are naturally induced and identified, the comprehensive indexes of the rice blast are all about 3.0, and the rice blast has excellent performance, abundance, resistance and wide adaptability. The Yangliangyou 512 participates in a late maturing group test of the indica in the middle and lower reaches of the Yangtze river in the southern rice region in 2017 plus 2018 year; quanyou 1512 participates in 2016-plus 2017 year green channel region test of indica late maturing group in middle and lower reaches of Yangtze river in the southern rice region of China, 2017 year green channel production test of indica late maturing group in middle and lower reaches of Yangtze river in the southern rice region of China, large-area demonstration planting is carried out at the same time, and the test is approved by the national crop variety approval committee in 2018 year.
2) Characteristic characteristics of Yangliangyou 512
Belongs to indica type two-line hybrid rice variety. Middle-season rice is planted in the middle and lower reaches of Yangtze river, the whole growing period is 136.1 days, and the Bifeng Shuangyou No. four (CK) is late-maturing for 0.4 days. The average plant height is 122.1 cm, the number of effective spikes per mu is 16.8 ten thousand, the spike length is 25.8 cm, the total number of the spikes is 217.9, the seed setting rate is 79.1%, and the thousand-grain weight is 23.5 g. Resistance: the rice blast comprehensive index is 3.6 grade, and the highest panicle blast loss rate is 5 grade; grade 7 of bacterial leaf blight; brown planthopper grade 7. The main indexes of the rice quality are as follows: the brown rice rate is 79.8%, the whole rice rate is 58.7%, the aspect ratio is 3.2, the chalky grain rate is 18.7%, the chalky degree is 3.0%, the transparency is 2 grade, the alkali digestion value is 3.4 grade, the gel consistency is 75mm, the amylose content is 13.4%, and the comprehensive rating is general in ministerial marks. In 2017, the average yield per mu of domestic district tests is 649.06 kg, the yield is increased by 7.17% compared with the yield of Fengshi-you-No. four (CK), and the yield increasing point proportion is 92.9%.
2) Characteristic features of Quanyou 1512
Belongs to indica type three-line hybrid rice varieties. The middle-season rice is planted in the middle and lower reaches of Yangtze river, the whole growing period is 133.7 days, and Bifeng Shuangyou No. four (CK) is precocious for 1.0 day. The average height of the plant is 126.9 cm, the effective spike number per mu is 15.7 ten thousand, the spike length is 26.2 cm, the total number of the spikes is 192.0, the seed setting rate is 83.6 percent, and the thousand seed weight is 27.3 g. Resistance: the rice blast comprehensive index is 4.0 grade, and the highest panicle blast loss rate is 7 grade; grade 5 of bacterial leaf blight; brown planthopper grade 9; heat resistance at heading stage 3 grade. Main indexes of rice quality are as follows: the whole rice rate is 59.3%, the length-width ratio is 3.2, the chalky grain rate is 26%, the chalky degree is 8.0%, the gel consistency is 86 mm, and the amylose content is 13.8%. In 2016, the rice is participated in green channel region tests of late ripening groups of middle and lower indica rice in the Yangtze river of the southern rice region of China, the average yield per mu is 665.04 kg, the yield is increased by 6.96 percent compared with the yield of Fengliou No. four (CK), and the level reaches extremely remarkable level; the average yield per mu of the subsequent test in 2017 is 631.41 kg, and the yield is increased by 3.29 percent compared with that of Fengshi-you-No. four (CK), so that the extremely remarkable level is achieved; the average yield per mu of a two-year district test is 648.23 kg, and the yield is increased by 4.99 percent compared with the yield of Fengliangyou No. four (CK); in a synchronous production test in 2017, the average yield per mu is 624.99 kg, and the yield is increased by 5.60 percent compared with the yield of Fengshi-you-No. four (CK), so that the level is extremely obvious.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims (5)

1. A breeding method of a strong rice blast resistance indica rice restorer line is characterized by comprising the following steps:
1) hybridizing the rice blast resistance indica rice restorer line parent I with excellent comprehensive agronomic characters, strong superiority and good combining ability as a female parent and the rice blast resistance indica rice restorer line parent II with excellent comprehensive agronomic characters, strong superiority and good combining ability as a male parent to obtain F1Seed generation; the first parent is Huazhan; parent II is radia Hui 838;
2) using the first parent as recurrent parent and F1Backcrossing once to obtain BC1F1Seed, detection of BC Using molecular markers1F1The rice blast resistance gene contained in the first parent in the group is investigated for agronomic characters, a single plant which contains the rice blast resistance gene and has the agronomic characters superior to the first parent is selected and mixed to obtain BC1F2Generation;
3) big group planting BC1F2Planting 500 plants, detecting the rice blast resistance gene contained in a parent through molecular markers, investigating the agronomic characters, integrating the molecular marker detection result and the agronomic character selection, and harvesting and selecting a single plant BC1F3Seed, establishing line BC1F3；
4) Planting BC by strains1F3Using parent as contrast, synthesizing molecular marker assisted selection MAS detection result, agronomic character selection, pedigree breeding, selecting single plant BC in harvest1F4Seed, and continuously establishing strain BC1F4；
5) Carrying out molecular marker detection on rice blast resistance genes contained in the first parent generation by generation, selecting a single plant with main agronomic characters superior to the first parent generation, and repeating the step 4) until BC is reached1F7Selecting a strain line containing rice blast resistance genes and having main agronomic characters superior to those of the parent I in the generation, carrying out test cross on the strain line by using pearl indica 97A, and harvesting BC1F8Generation seeds and corresponding test cross seeds;
6) paired planting of BC1F8The strain is tested and cross-bred with the test cross-bred strain,identification of heterosis, respectively on Stable BC1F8Performing resistance identification on the strains through seedling plague and panicle plague; synthesizing heterosis, main agronomic characters and rice blast resistance identification results, selecting stable strains meeting breeding targets, breeding new rice blast resistance strong dominance indica rice restoring lines, and detecting rice blast resistance genes contained in parent lines by using molecular markers in steps 2), 3), 4) and 5)Pi2、Pi54、Pid3、PitaAndPib。
2. the breeding method of the rice blast resistant strong dominant indica rice restorer line according to claim 1, characterized in that: collecting rice ears naturally infected with rice blast from rice blast re-infected areas in step 6), culturing pathogenic bacteria, selecting representative strains, expanding culture, eluting rice blast spores, and adjusting concentration to 2 × 105each.mL-1As an inoculum for identifying rice blast resistance.
3. The breeding method of the rice blast resistant strong dominant indica rice restorer line according to claim 1, characterized in that: the breeding method further comprises the following steps:
7) expanding and propagating new rice blast resistant strong dominant indica rice restorer line seeds, simultaneously, taking the indica rice restorer line seeds as male parents, taking different sterile lines as female parents, configuring a hybridization combination, identifying the matching advantages of the bred rice blast resistant strong dominant restorer line, identifying the field resistance in different rice blast disease gardens, synthesizing the panicle blast and disease garden resistance identification results, and comprehensively evaluating the rice blast resistance of the bred restorer line and the hybridization combination configured by taking the indica rice restorer line seeds as male parents.
4. A method for breeding a new rice blast-resistant high-quality high-yield two-line hybrid indica rice combination is characterized by comprising the following steps: on the basis of breeding a rice blast resistant indica rice restorer line with strong dominance according to the breeding method of claim 1, different sterile lines are used as female parents to configure a hybrid combination, the rice blast strains used in the step 6) of claim 1 are inoculated in the booting stage of the hybrid progeny under the premise of ensuring that the yield is high and the yield is increased, the panicle blast resistance of the hybrid combination is identified, and meanwhile, the rice blast resistance of the configured hybrid combination is comprehensively evaluated by combining the resistance identification results of different rice blast disease gardens; harvesting rice of hybrid progeny of all the hybrid combinations, and detecting the rice quality of all the hybrid combinations according to the national standard of high-quality rice; finally, the new rice blast-resistant hybrid indica rice combination with high quality and high yield is obtained.
5. The breeding method according to claim 4, wherein the different disease nursery is selected from 4 disease nursery of rice blast in Hangzhou, Jiangxi Jinggang mountain, Anhui Jinzhai and Enshi in Hubei province.
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