CN110607382A - SNP molecular marker of single ring weight major gene derived from Xinluzao 24 - Google Patents

SNP molecular marker of single ring weight major gene derived from Xinluzao 24 Download PDF

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CN110607382A
CN110607382A CN201811052726.4A CN201811052726A CN110607382A CN 110607382 A CN110607382 A CN 110607382A CN 201811052726 A CN201811052726 A CN 201811052726A CN 110607382 A CN110607382 A CN 110607382A
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李俊文
巩万奎
刘瑞贤
龚举武
葛群
刘爱英
石玉真
商海红
袁有禄
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Abstract

The invention discloses a molecular marker closely linked with major QTL sites qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the single boll weight of upland cotton. Then, the cotton SNP80K chip is used for carrying out genotyping and genetic linkage map construction on the population. Major sites influencing the single-boll weight of cotton and SNP molecular markers closely linked with the sites are obtained through QTL positioning. In the cotton breeding process, the molecular markers are used for auxiliary selection, so that the single boll weight of a new variety of upland cotton can be effectively and pertinently improved, and the high-yield and high-quality breeding efficiency is improved.

Description

SNP molecular marker of single ring weight major gene derived from Xinluzao 24
Technical Field
The invention belongs to the field of biotechnology application, and relates to upland cotton Single boll weight major QTLs and Single Nucleotide Polymorphism (SNP) molecular markers linked with the same.
Background
Cotton is an important economic crop of the world, and cotton fiber is an important raw material for the textile industry and is widely grown in over 80 countries (Jamshed et al, 2016). A crop that is also the source of the second most important edible oils and proteins (Zhang et al, 2014 b). Four cultivars of the cotton genus (Gossypium) (g. herbarum, g. arboreum, g. hirsutum and g. barbadene) are used for cotton fiber production (Li et al, 2014,2015; Wendel et al, 2015; Zhang et al, 2015), of which tetraploid uploid cotton upland (Gossypium g. hirsutum L) and sea island cotton (g. barbadene) are the main cultivars. Although the quality and disease resistance of upland cotton fibers are general, the upland cotton fibers have the characteristics of high yield potential, wide adaptability and the like, and the planting area of the upland cotton fibers still accounts for more than 95% of the total cotton planting area (Cai et al, 2014; Chen et al, 2007). With the continuous improvement of living standard and textile technology, the requirements for yield are higher and higher, and diversified requirements for the quality of cotton fibers, such as high-strength fibers, natural colored cotton and the like, are provided. The cotton yield and fiber quality traits are quantitative traits and are controlled by polygenes (Said et al, 2013), and the traits are mostly in negative correlation relationship (Shen et al, 2007; Wang et al, 2015). Therefore, it is difficult to improve these traits simultaneously by conventional breeding methods, and a large amount of time and labor cost are required (Shen et al, 2005; Lacape et al, 2009; Jamshed et al, 2016; Zhang et al, 2016). The rapid development of applied genomics research provides an effective tool for improving breeding efficiency, and the most typical examples are molecular marker-assisted selection and breeding by genome selection or molecular design through molecular markers closely linked to target genes.
Previous QTL mapping studies have focused primarily on cotton fiber quality traits (Fang et al, 2014; Li et al, 2016; Liu et al, 2017; Jamshed et al, 2016; Shen et al, 2005; Sun et al, 2012 a; Tan et al, 2015; Wang et al,2015 a; Xu et al, 2014; Yang et al, 2016; Zhang et al, 2017b), disease resistance (Jiang et al, 2009; palagaga et al, 2017; Ulloa et al, 2013; Zhao et al, 2014), early maturity (Li et al, 2012; 2013; Stiller et al, 2004), plant type (Qi et al, 2017; Tang et al, 2014), and the like. There are few reports relating to the production of, in particular, the formation of bell weights. The boll weight is an important component of the yield development of upland cotton. Qin et al (2008) used the four-way cross population to locate a cotton boll weight QTL on chromosome D2 that linked three SSR markers spanning a genetic distance of 14.5 cM. Liu et al (2012) used the data from the four environments of the recombinant inbred population to locate 8 bell-weight QTLs distributed across 15 chromosomes. Yu et al (2013) located 10 QTLs distributed on 8 chromosomes using the population of the backcross line between upland cotton and sea island cotton. Zhang et al (2016) located 16 multi-ambient-stable bell weight QTLs using a recombinant inbred population. These QTLs would be of great significance for cotton yield improvement.
Although the corresponding QTL was successfully mapped, there are few reports of QTL and markers successfully applied to molecular marker assisted selection. The main reasons for this are: 1) most of the early-stage located QTL is based on genetic maps with low saturation and coverage, and the located QTL has large intervals, so that the application of the QTL in marker-assisted selection is influenced. 2) An episodic QTL cannot be detected across populations, i.e. a QTL that is localized in one population may not be detected in another population. 3) There are few located multi-environmentally stable QTLs. These phenomena all affect the utility of these QTLs and their linked markers (Wei et al, 2014; Guo et al, 2015; Ma et al, 2015; Zhang et al, 2016).
Recently, a successfully developed Cotton SNP80K chip (Cai et al, 2017) is chip data developed after 100 gossypium hirsutum are re-sequenced based on genome data of a gossypium hirsutum standard system Tm-1, and therefore, the chip data may have more practical application value for related research of gossypium hirsutum (Cai et al, 2017). The total map distance for QTL positioning in the invention is 2477.99cM, and comprises 122 SSR markers and 4729 SNP markers, and the average distance between the markers is 0.51 cM. Compared with the prior map, the saturation of the map is greatly improved, and QTL positioning can be more effectively carried out (Liu et al, 2015; Li et al, 2016; Zhang et al, 2016; 2017; Tan et al, 2018).
The means for improving the yield and quality of upland cotton in the traditional breeding method is mainly to select according to the phenotype of yield and fiber quality in the breeding process of hybrid offspring. This requires the selection of a population large enough for the selection of individuals in the growth period, indoor examination and corresponding fiber quality instrumental detection and identification of the selected individuals in the later period, and then individual selection. And the indoor test of the individual plant and the detection and identification of the fiber quality by an instrument are carried out in each selection generation. This requires a high investment in labor, time and capital costs. And the yield character of cotton is the quantitative character controlled by multiple genes, and the phenotype of the cotton is greatly influenced by the environment, so that the selection effect and efficiency are greatly influenced. Cotton single boll weight is also a quantitative trait, and its phenotype is also influenced by the environment. Therefore, the single bell weight molecular marker technology can carry out a large amount of individual or family detection in a short time, and because the genotype of the molecular marker can be identified, if the target character is closely linked with a certain molecular marker, the molecular marker can be used for bell weight selection, thereby greatly improving the efficiency of detection and selection.
Disclosure of Invention
The invention obtains SNP molecular markers which are from upland cotton seeds and are closely linked with the single bell weight through the genetic linkage map of a recombination inbred line population between upland cotton high-yield varieties (Lu cotton research 28 and Xin Lu Zao 24) and the phenotypic identification of the single bell weight of the population, and by utilizing the molecular markers which are closely linked with the bell weight, the single bell weight can be directly selected at the DNA level by a separation generation in the breeding process without selecting the phenotype which is detected and identified through later-stage seed test, thereby improving the selection efficiency and effect. The inventor finds that the recombinant inbred line group generates a series of recombinant inbred lines with different single-bell weights in a separated way under the natural condition of a field in the process of constructing the recombinant inbred line group by utilizing Xinluzao 24 and Lu cotton research 28 and identifying the field, and the phenotype expression of the single-bell weights of the inbred lines is stable. The inventor locates 5 multi-environment-stable single-bell QTLs by constructing a genetic map of the segregation population and identifying relevant phenotypes thereof.
The technical scheme provided by the invention is as follows: the molecular markers closely linked with the major gene loci qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the upland cotton single boll weight, wherein the major gene loci qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 are respectively and sequentially located on the 4 th, 5 th, 6 th, 21 th and 24 th chromosomes of upland cotton, and the markers linked with qBW-chr04-2 are as follows: TM9831, TM 9827; markers linked to qBW-chr05-5 are: TM10953, TM10955, TM10930, TM 10979; markers linked to qBW-chr06-3 include: TM17001, TM14514, TM14512, TM14465, TM14428, TM14429, TM14432, TM14433, TM14434, TM14430, TM14446, TM14443, TM14437, TM14436, TM14435, TM14438, TM14441, TM14448, TM14493, TM14477, and TM 14509; markers linked to qBW-chr21-4 are: TM53503, TM53502, TM53501, TM53504, TM53571, and TM 53577; the markers linked to qBW-chr24-1 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. Markers linked to qBW-chr21-4 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887.
Wherein the meanings of the 5 cotton single-boll weight major gene loci are as follows: qBW-chr04-2 shows that the 2 nd locus located on the upland cotton No. 4 chromosome is a major gene locus; qBW-chr05-5 shows that the 5 th site located on the upland cotton chromosome 5 is the major site; and so on. The meaning of the markers closely linked to the major gene locus is: taking TM10953 as an example, the SNP marker is shown, and the number of the SNP marker is TM10953 in a cotton 80KSNP chip, and the TM shows that the marker develops sequencing data derived from a upland cotton standard line TM-1.
The molecular marker linked with the upland cotton single boll weight major gene locus is obtained by the following method:
1) constructing a recombinant inbred line group containing 231 inbred lines by using a large-area popularized upland cotton cultivation variety Lu cotton research 28 with high yield potential and a high-quality upland cotton variety Xinluzao 24 as parents;
2) the genotype analysis of the recombinant inbred line population and the parents thereof is carried out by utilizing a cotton 80KSNP chip, and 4729 SNP markers with polymorphism between two parents and in the recombinant inbred line population are obtained. Simultaneously, 122 SSR markers with polymorphism between two parents and in a recombinant inbred line population are selected, and a genetic linkage map which comprises 4851 markers in total is constructed;
3) under the field production condition, the phenotype of the yield character of the recombinant inbred line population is investigated and identified in multiple environments under 9 environmental conditions of a test field (2013, 2014,2015, 2016) of a cotton institute of Chinese agricultural sciences (Henan Anyang), a test station (2013, 2014) of Shandong cotton center Linqing, a Weeku test station (2013) of Chinese agricultural university, a Xinjiang Korla test base (2014) of Chinese agricultural sciences and a cotton research base (2015) of Xinjiang Arael of Chinese agricultural sciences.
4) And according to the identification result of the yield trait phenotype of the recombinant inbred line population, positioning the single-bell-weight major gene locus/QTL in multiple environments by using the 4851-marker-containing genetic linkage map. 5 multi-environment-stable single-ring-weight major QTLs are positioned. Namely: qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr 24-1. The single bell weight synergistic genes are all from Xinluzao 24. Markers linked to qBW-chr04-2 are: TM9831, TM 9827; markers linked to qBW-chr05-5 are: TM10953, TM10955, TM10930, TM 10979; markers linked to qBW-chr06-3 include: TM17001, TM14514, TM14512, TM14465, TM14428, TM14429, TM14432, TM14433, TM14434, TM14430, TM14446, TM14443, TM14437, TM14436, TM14435, TM14438, TM14441, TM14448, TM14493, TM14477, and TM 14509; markers linked to qBW-chr21-4 are: TM53503, TM53502, TM53501, TM53504, TM53571, and TM 53577; the markers linked to qBW-chr24-1 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. Markers linked to qBW-chr21-4 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887.
The molecular markers related to the major gene sites qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the upland cotton single boll weight are shown in the following table 1 as characteristic sequences, positions in a physical map and SNP mutation sites:
table 1: qBW location on genetic and physical maps, SNP flanking sequence (the base in parentheses is SNP locus), and parental genotype
The invention has the following advantages:
the invention relates to 5 sites (qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1) related to the major gene of the single bell weight of upland cotton, and the synergistic gene of the single bell weight is derived from Xinluzao 24. The 5 major gene loci all show multi-environment stabilization effect and can be used for molecular marker-assisted selection of upland cotton single boll weight. qBW-chr04-2 explained phenotypic variation between 3.77% and 5.74% with additive effect between 0.13 and 0.16 (g); qBW-chr05-5 explained phenotypic variation between 4.28% and 6.42%, additive effect between 0.11-0.13 (g); qBW-chr06-3 explained phenotypic variation of 3.87-4.07% with additive effect of 0.09-0.10 (g); qBW-chr21-4 explained phenotypic variation of 6.05% -7.26%, additive effect of 0.11 (g); qBW-chr24-1 explained phenotypic variation of 4.13% -6.04%, additive effect of 0.11-0.14 (g).
The invention constructs the genetic linkage map of the genetic markers in the recombinant inbred line population through the genotypes of the molecular markers, and identifies the phenotype of the population on the yield traits including the bell weight under the multi-environment condition. The genotype and phenotype of the genetic map are combined and analyzed to obtain the molecular marker related to the ring weight. The molecular markers closely linked with the boll weight are used for auxiliary selection, so that the boll weight improvement of the upland cotton variety can be pertinently carried out, and the high-yield and high-quality breeding efficiency of the upland cotton can be improved.
Drawings
FIG. 1 shows the molecular marker linkage map and major gene locus/QTL of the recombinant inbred line population of the invention. These major gene loci are located on chromosomes 4, 5, 6, 21 and 24, respectively.
Among them, qBW-chr04-2 linked markers are: TM9831, TM 9827; markers linked to qBW-chr05-5 are: TM10953, TM10955, TM10930, TM 10979; markers linked to qBW-chr06-3 include: TM17001, TM14514, TM14512, TM14465, TM14428, TM14429, TM14432, TM14433, TM14434, TM14430, TM14446, TM14443, TM14437, TM14436, TM14435, TM14438, TM14441, TM14448, TM14493, TM14477, and TM 14509; markers linked to qBW-chr21-4 are: TM53503, TM53502, TM53501, TM53504, TM53571, and TM 53577; the markers linked to qBW-chr24-1 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. Markers linked to qBW-chr21-4 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887.
Detailed Description
The present invention will be further illustrated by the following detailed description of embodiments, wherein the molecular markers linked to the major gene of upland boll weight are obtained by the following method:
(1) the cultivation method and process of the recombinant inbred line for identifying the weight of the cotton bolls on land and constructing the genetic linkage map comprises the following steps: a large-area popularized upland cotton cultivar Lu Cotton research 28 with high yield potential cultivated by Shandong cotton research center is used as a female parent, a high-quality variety Xinluzao 24 cultivated by Xinjiang Kangdi company is used as a male parent, a hybrid combination is configured on a cotton research institute (Henan Anyang) test farm of Chinese academy of agricultural sciences in summer of 2008, and F is planted in Hainan of 20081. Planting 238F plants in Anyang in spring of 20092Individual plants, selfing to obtain F23 families. 231F are selected23 lines, breeding in Hainan in winter, selfing each generation, selecting a selfing bell for each plant in the lines, and harvesting until F26 at F26 randomly selecting a single plant from the 6 families, selfing for 2 generations to obtain F6The 8 generation population. F is to be6The 8 generation and later populations are used as Recombinant Inbred Line (RIL) populations.
(2) Taking a recombinant inbred line population (F)6: 8) the DNA of 231 families and amphiphilic Xinluzao 24 and Lumian 28 were extracted from the leaf samples of (1).
(3) The inbred line population and the parents thereof are subjected to genotype analysis by using a cotton 80KSNP chip, and 4729 SNP markers having polymorphism between two parents and in a recombinant inbred line population are obtained. Simultaneously, 122 SSR markers with polymorphism between two parents and in a recombinant inbred line population are selected. In the invention, the molecular markers related to the major gene sites qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the upland cotton single boll weight have the characteristic sequences, the positions in a physical map and SNP mutation sites shown in Table 1:
table 1: qBW location on genetic and physical maps, SNP flanking sequence (the base in parentheses is SNP locus), and parental genotype
(4) Genetic linkage maps were constructed using HighMap mapping software (Liu et al, 2014) and the distances between the maps were estimated using Kosambi functions (Kosambi, 1943). A genetic linkage map containing 4851 marker loci (including 4729 SNP markers and 122 SSR markers) is constructed, 26 linkage groups are constructed in total, all the linkage groups are positioned on 26 chromosomes of upland cotton, 2477.99cM is covered on a upland cotton genome, and the average inter-marker distance is 0.51 cM. Wherein subgroup A contains 3300 markers covering the genome 1474.63cM, with an average inter-marker distance of 0.45 cM. Subgroup D contains 1551 molecular markers, covering 1003.36cM genome. The average inter-marker distance was 0.65 cM. The molecular marker linkage map and major gene locus/QTL of the recombinant inbred line population of the invention are shown in figure 1.
(5) Combining the identification result of the single bell weight of the recombination inbred line population, carrying out QTLs positioning by utilizing Windows QTL Cartogpher 2.5, carrying out 1000 times of sequencing test, screening the single bell weight main effect QTL stably expressed in multiple environments, screening 5 main single bell weight QTLs, qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1, respectively and sequentially locating on No. 4, No. 5, No. 6, No. 21 and No. 24 chromosomes of upland cotton, and the marker linked with qBW-chr04-2 comprises: TM9831, TM 9827; markers linked to qBW-chr05-5 are: TM10953, TM10955, TM10930, TM 10979; markers linked to qBW-chr06-3 include: TM17001, TM14514, TM14512, TM14465, TM14428, TM14429, TM14432, TM14433, TM14434, TM14430, TM14446, TM14443, TM14437, TM14436, TM14435, TM14438, TM14441, TM14448, TM14493, TM14477, and TM 14509; markers linked to qBW-chr21-4 are: TM53503, TM53502, TM53501, TM53504, TM53571, and TM 53577; the markers linked to qBW-chr24-1 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. Markers linked to qBW-chr21-4 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. The single bell weight synergistic genes are all from Xinluzao 24. The 5 major gene loci all show multi-environment stabilization effect and can be used for molecular marker-assisted selection of upland cotton single boll weight. qBW-chr04-2 explained phenotypic variation between 3.77% and 5.74% with additive effect between 0.13 and 0.16 (g); qBW-chr05-5 explained phenotypic variation between 4.28% and 6.42%, additive effect between 0.11-0.13 (g); qBW-chr06-3 explained phenotypic variation of 3.87-4.07% with additive effect of 0.09-0.10 (g); qBW-chr21-4 explained phenotypic variation of 6.05% -7.26%, additive effect of 0.11 (g); qBW-chr24-1 explained phenotypic variation between 4.13% and 6.04% with additive effect between 0.11-0.14 (g).

Claims (4)

1. Molecular markers closely linked with major gene loci qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the upland cotton single boll weight, and is characterized in that the major gene loci qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 of the upland cotton single boll weight are respectively and sequentially located on chromosomes 4, 5, 6, 21 and 24 of the upland cotton; the marker interval linked with qBW-chr04-2 is TM9831-TM 9827; the marker interval linked with qBW-chr05-5 is TM10953-TM 10979; the marker interval linked with qBW-chr06-3 is TM17001-TM 14509; the marker interval linked with qBW-chr21-4 is TM76018-TM 75887; the marker interval linked with qBW-chr24-1 is TM67152-TM 67127.
2. The molecular markers closely linked to upland cotton single boll major gene loci qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr21-4 and qBW-chr24-1 as claimed in claim 1, which is obtained by:
1) the method comprises the steps of preparing a hybrid combination by using a high-yield upland cotton cultivation variety Lu cotton research 28 popularized in a large area in a yellow river basin cotton area as a female parent and an upland cotton high-quality variety Xinluzao 24 popularized in a large area in Xinjiang as a male parent, and breeding a recombinant inbred line group containing 231 inbred lines through multi-generation inbred breeding;
2) carrying out genotype analysis on the recombinant inbred line population and the parents thereof by using a upland cotton SNP80K chip to obtain 4729 SNP markers with polymorphism between two parents and between the recombinant inbred lines in the recombinant inbred line population; and simultaneously carrying out polymorphism analysis on the two parents by using SSR markers, and carrying out genotyping on the recombinant inbred line population by using the SSR markers with polymorphism between the two parents to obtain 122 SSR markers in total. Constructing a genetic linkage map containing 4851 markers and having a total map distance of 2477.99 cM;
3) under the natural production condition of a field, performing multi-environment phenotypic identification on the single cotton boll weight of the recombinant inbred line population in two ecological areas, namely a northwest inland cotton area and a yellow river valley;
4) according to the single-boll phenotype identification result of the recombinant inbred line population under the field natural production condition, the genetic linkage map containing 4851 markers is utilized to carry out single-boll major QTL positioning of upland cotton under multiple environments, and 5 multi-environment-stable QTLs related to the single-boll weight of the upland cotton are positioned together, namely: qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr07-4, qBW-chr20-1, qBW-chr21-4 and qBW-chr 24-1; the bell weight synergistic genes are all from Xinluzao 24, and the qBW-chr04-2 linked markers comprise: TM9831, TM 9827; markers linked to qBW-chr05-5 are: TM10953, TM10955, TM10930, TM 10979; markers linked to qBW-chr06-3 include: TM17001, TM14514, TM14512, TM14465, TM14428, TM14429, TM14432, TM14433, TM14434, TM14430, TM14446, TM14443, TM14437, TM14436, TM14435, TM14438, TM14441, TM14448, TM14493, TM14477, and TM 14509; markers linked to qBW-chr21-4 are: TM53503, TM53502, TM53501, TM53504, TM53571, and TM 53577; the markers linked to qBW-chr24-1 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887. Markers linked to qBW-chr21-4 are: TM76018, TM76009, TM76030, TM76010, TM76033, TM76021, TM76006, TM76007, TM76012, TM76000, TM76014, TM76017, TM76027, TM75885, and TM 75887.
3. The molecular markers related to upland cotton single bell weight major gene sites qBW-chr04-2, qBW-chr05-5, qBW-chr06-3, qBW-chr07-4, qBW-chr20-1, qBW-chr21-4 and qBW-chr24-1 of claim 2, wherein the characteristic sequences, the positions in the physical map and the SNP mutation sites are shown in the following table one:
table one: qBW location on genetic and physical maps, SNP flanking sequences and parental genotypes
4. Use of a molecular marker as claimed in any one of claims 1 to 3 in cotton assisted breeding to increase cotton single boll weight and hence cotton lint yield.
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* Cited by examiner, † Cited by third party
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CN113373254A (en) * 2021-06-11 2021-09-10 中国农业大学 Molecular marker linked with salt-tolerant QTL of upland cotton and application thereof
CN115820892A (en) * 2022-07-04 2023-03-21 中国农业科学院棉花研究所 SNP molecular marker associated with upland cotton chromosome A07 and boll weight and application thereof
CN117286286A (en) * 2023-11-23 2023-12-26 南京农业大学三亚研究院 Molecular marker closely linked with cotton boll weight character and application thereof

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CN106929574A (en) * 2017-02-22 2017-07-07 中国农业科学院棉花研究所 No. 4 chromosomes of upland cotton SNP marker related to fibre strength

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113373254A (en) * 2021-06-11 2021-09-10 中国农业大学 Molecular marker linked with salt-tolerant QTL of upland cotton and application thereof
CN113373254B (en) * 2021-06-11 2023-02-21 中国农业大学 Group of molecular markers linked with salt-tolerant QTL of upland cotton and application
CN115820892A (en) * 2022-07-04 2023-03-21 中国农业科学院棉花研究所 SNP molecular marker associated with upland cotton chromosome A07 and boll weight and application thereof
CN117286286A (en) * 2023-11-23 2023-12-26 南京农业大学三亚研究院 Molecular marker closely linked with cotton boll weight character and application thereof
CN117286286B (en) * 2023-11-23 2024-02-20 南京农业大学三亚研究院 Molecular marker closely linked with cotton boll weight character and application thereof

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