CN109554492B - SNP molecular marker of fiber strength major gene from Xinluzao 24 and Lumian 28 - Google Patents

SNP molecular marker of fiber strength major gene from Xinluzao 24 and Lumian 28 Download PDF

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CN109554492B
CN109554492B CN201811045873.9A CN201811045873A CN109554492B CN 109554492 B CN109554492 B CN 109554492B CN 201811045873 A CN201811045873 A CN 201811045873A CN 109554492 B CN109554492 B CN 109554492B
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巩万奎
刘瑞贤
李俊文
龚举武
葛群
刘爱英
石玉真
商海红
袁有禄
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Abstract

The invention discloses a molecular marker closely linked with major QTL sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the fiber strength of upland cotton. Meanwhile, the cotton SNP80K chip is used for carrying out genotyping and genetic linkage map construction on the population. The major effect sites influencing the cotton fiber strength and SNP molecular markers closely linked with the major effect sites are obtained through QTL positioning. When the molecular markers are used for auxiliary selection, the fiber strength of upland cotton varieties can be effectively and pertinently improved, and the high-yield and high-quality breeding efficiency is improved.

Description

SNP molecular marker of fiber strength major gene from Xinluzao 24 and Lumian 28
Technical Field
The invention belongs to the field of biotechnology application, and relates to upland cotton fiber strength major QTLs and Single Nucleotide Polymorphism (SNP) and Simple Sequence Repeat (SSR) molecular markers linked with the same.
Background
Cotton is an important economic crop of the world, and cotton fiber is an important raw material for the textile industry and is widely grown in over 80 countries (Jamshed et al, 2016). A crop that is also the source of the second most important edible oils and proteins (Zhang et al, 2014 b). Four cultivars of the cotton genus (Gossypium) (g. herbarum, g. arboreum, g. hirsutum and g. barbadene) are used for cotton fiber production (Li et al, 2014,2015; Wendel et al, 2015; Zhang et al, 2015), of which tetraploid uploid cotton upland (Gossypium g. hirsutum L) and sea island cotton (g. barbadene) are the main cultivars. Although the quality and disease resistance of upland cotton fibers are general, the upland cotton fibers have the characteristics of high yield potential, wide adaptability and the like, and the planting area of the upland cotton fibers still accounts for more than 95% of the total cotton planting area (Cai et al, 2014; Chen et al, 2007). With the continuous improvement of living standard and textile technology, the requirements for yield are higher and higher, and diversified requirements for the quality of cotton fibers, such as high-strength fibers, natural colored cotton and the like, are provided. The cotton yield and fiber quality traits are quantitative traits and are controlled by polygenes (Said et al, 2013), and the traits are mostly in negative correlation relationship (Shen et al, 2007; Wang et al, 2015). Therefore, it is difficult to improve these traits simultaneously by conventional breeding methods, and a large amount of time and labor cost are required (Shen et al, 2005; Lacape et al, 2009; Jamshed et al, 2016; Zhang et al, 2016). The rapid development of applied genomics research provides an effective tool for improving breeding efficiency, and the most typical examples are molecular marker-assisted selection and breeding by genome selection or molecular design through molecular markers closely linked to target genes.
A series of segregating populations within upland cotton species have been established to date, with various traits aimed at cotton including fiber quality ((Fang et al, 2014; Li et al, 2016; Liu et al, 2017; Jamshed et al, 2016; Shen et al, 2005; Sun et al, 2012 a; Tan et al, 2015; Wang et al,2015 a; Xu et al, 2014; Yang et al, 2016; Zhang et al, 2017b)), yield (Liuu et al, 2017; Wang et al,2015 a; Xia et al, 2014; Zhang et al, 2016), drought tolerance (Levi et al, 2011), disease resistance (Jiai et al, 2009; Palanga et al, 2017; Ulloa et al, 2013; ZHao et al, 2014), early maturity (Li et al, 2012; 2013; Stiller et al, 2004), plant type (Tang et al, 2017; Qi et al, 2014), etc. Shen et al (2007) mapped 7 fiber strength QTLs, which could account for 4.31-16.15% of phenotypic variation. Sun et al (2012) mapped to a QTL of 40 fiber strength using a population of recombinant inbred lines, which could account for 1.60-27.86% of the phenotypic variation. Ning et al (2014) mapped a QTL of 23 fiber strengths that could account for 3.73-17.55% of the phenotypic variation. Fang et al (2014) mapped to a QTL of 20 fiber strengths and Zhang (2017) mapped to a QTL of 16 multi-ring ambient stable fiber strengths.
Although the corresponding QTL was successfully mapped, there are few reports of QTL and markers successfully applied to molecular marker assisted selection. The reason for this is that: 1) most of the early-stage located QTL are linked genetic maps constructed based on SSR markers, the relatively low polymorphism of SSR enables the saturation and coverage of the maps obtained by the early-stage located QTL to be low, and the located QTL has a large interval, so that the application of the early-stage located QTL in marker-assisted selection is influenced. Another reason is that QTLs localized by one population may not be detected in another population, which also affects the use of these QTLs and their linked markers (Wei et al, 2014; Guo et al, 2015; Ma et al, 2015; Zhang et al, 2016).
Recently, two sets of successful SNP chip studies (CottonSNP63K and CottonSNP80K) (Hulse-Kemp et al, 2015; Cai et al, 2017) have been applied to cotton studies. The CottonSNP80K chip is the chip data developed after 100 upland cotton are re-sequenced based on the genome data of the standard line Tm-1 of upland cotton, so that the CottonSNP chip is likely to have more practical application value in the related research of upland cotton (Cai et al, 2017). The total map distance for QTL positioning in the invention is 2477.99cM, and comprises 122 SSR markers and 4729 SNP markers, and the average distance between the markers is 0.51 cM. Compared with the prior map, the saturation of the map is greatly improved, and QTL positioning can be more effectively carried out (Liu et al, 2015; Li et al, 2016; Zhang et al, 2016; 2017; Tan et al, 2018).
The means for improving the fiber quality of upland cotton varieties in the traditional breeding method is mainly to select according to the phenotype of the fiber quality in the breeding process of hybrid offspring, and the fiber quality identification needs to be detected and identified indoors through an instrument after harvesting. This requires the selection of a population large enough for the indoor instrumental evaluation of fiber quality prior to individual selection during the growth period. And the quality of the fiber is detected and identified by indoor instruments in each selection generation. This requires a high investment in labor, time and capital costs. And the fiber quality character of cotton is a quantitative character controlled by multiple genes, and the phenotype of the cotton is greatly influenced by the environment, so that the selection effect and efficiency are greatly influenced. The molecular marker technology can carry out a large amount of individual or family detection in a short time, and because the genotype of the molecular marker can be identified, if the target character is closely linked with a certain molecular marker, the molecular marker can be used for selecting the target character, thereby greatly improving the efficiency of detection and selection.
Disclosure of Invention
The invention obtains SNP molecular markers which are from upland cotton seeds and are closely linked with fiber strength through genetic linkage maps of upland cotton recombinant inbred line groups and phenotype identification of the groups, and by utilizing the molecular markers which are closely linked with the fiber strength, fiber strength can be directly selected at a DNA level by a separation generation in a breeding process without selecting the phenotype which is detected and identified by a fiber strength instrument, thereby improving the selection efficiency and effect. The inventor finds that the recombinant inbred line group can be separated to generate a series of high-strength fiber-quality recombinant inbred lines under the natural conditions of the field, and the phenotype of the fiber strength of the inbred lines is stable in the process of constructing the recombinant inbred line group and identifying the field by using Xinluzao 24 and Lu cotton research 28. The inventor locates 9 multi-environment stable QTLs of fiber strength by constructing a genetic map of the segregation population and identifying relevant phenotypes thereof.
The technical scheme provided by the invention is as follows: and molecular markers closely linked with major gene loci qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the upland cotton high-strength fiber quality, wherein the major gene loci qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the upland cotton are respectively and sequentially located on chromosomes 1, 7, 11, 13, 20 and 24 of the upland cotton respectively. The marker linked to qFS-chr01-2 is TM 379-TM 404; the marker linked to qFS-chr07-1 is TM 19848-TM 19875; the marker linked to qFS-chr11-1 is TM 37826-TM 37828; the marker linked to qFS-chr11-2 is TM 37897-TM 37935; the marker linked to qFS-chr13-1 is TM 43230-TM 43229; the marker linked with qFS-chr20-1 is TM 75088-TM 75100; the marker linked to qFS-chr20-5 is TM 73152; the marker linked to qFS-c24-1 is TM 67152-TM 67146.
Wherein the ten cotton fiber strength major gene loci have the meanings as follows: qFS-chr01-2 shows that the second site of two fiber strength gene sites on the upland cotton No. 1 chromosome is a major gene site; qFS-chr07-1 shows that the first locus on upland cotton No. 7 chromosome is the major gene locus, and the rest is analogized; the meaning of the markers closely linked to the major gene locus is: taking TM19848 as an example, it is shown to be an SNP marker, numbered TM19848 in the Cotton80KSNP chip, and TM shows that the marker develops sequencing data derived from the upland Cotton standard line TM-1.
The molecular marker linked with the major gene locus of the upland cotton fiber strength is obtained by the following method:
1) constructing a recombinant inbred line group containing 231 inbred lines by using a large-area popularized upland cotton cultivation variety Lu cotton research 28 with high yield potential and a high-quality upland cotton variety Xinluzao 24 as parents;
2) by using a Cotton80KSNP chip, genotype analysis is carried out on the recombinant inbred line population and the parents thereof, and 4729 SNP markers with polymorphism between the two parents and in the recombinant inbred line population are obtained. Simultaneously, 122 SSR markers with polymorphism between two parents and in a recombinant inbred line population are selected, and a genetic linkage map which comprises 4851 markers in total is constructed;
3) under the field production condition, the phenotype of the fiber quality character of the recombinant inbred line population is investigated and identified in multiple environments under 9 environmental conditions of a test field (2013, 2014,2015, 2016) of a cotton institute of Chinese agricultural sciences (Henan Anyang), a test station (2013, 2014) of Shandong cotton center Linqing, a Weeku test station (2013) of Chinese agricultural university, a Xinjiang Korla test base (2014) of Chinese agricultural sciences and a cotton research base (2015) of Xinjiang Arael of Chinese agricultural sciences.
4) And according to the identification result of the fiber quality phenotype of the recombinant inbred line population, positioning the fiber strength major gene locus/QTL in multiple environments by using the 4851 marker-containing genetic linkage map. A total of 8 multi-environmental stable fiber strength major QTLs were located, namely: qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c 24-1. Wherein, fiber strength enhancing genes of qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1 and qFS-chr20-1 are derived from Xinluzao 24; the fiber strength enhancing genes of qFS-chr20-5 and qFS-c24-1 are from Lu cotton research 28. Markers linked to qFS-chr01-2 are: TM379, TM374, TM378, TM381, TM382, TM386, TM390, TM389, TM391, TM405, TM406, and TM 404; markers linked to qFS-chr07-1 are: TM19848, TM19877, TM19894, TM19872, TM19865, TM19873, TM19870, TM19855, TM19853, TM19859, TM19868, TM19862, and TM 19875; markers linked to qFS-chr11-1 are: TM37826, TM37831, TM37829, and TM 37828; markers linked to qFS-chr11-2 are: TM37897, TM37888, TM37893, TM37880, TM37894, TM37879, TM37878, TM37887, TM37875, TM37883, TM37884, TM37901, TM37904, TM37902, TM37917, TM37906, TM37907, TM37909, TM37908, TM37924, TM37929, TM37912, TM37919, TM37916, TM37918, TM37911, TM37931, TM37938, TM37932, TM37937, and TM 37935; markers linked to qFS-chr13-1 are: TM43230, TM43227, and TM 43229; markers linked to qFS-chr20-1 are: TM75088, TM75098, and TM 75100; markers linked to qFS-chr20-5 are: TM 73152; markers linked to qFS-chr24-1 are: TM67152 and TM 67146.
The molecular markers related to the major gene sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the cotton fiber strength in upland area are characterized in that the characteristic sequences, the positions in a physical map and SNP mutation sites are shown in Table 1:
TABLE 1 location of qFS on genetic and physical maps, SNP flanking sequences (where the bases in brackets are SNP sites), and parental genotypes
Figure BDA0001793281210000041
Figure BDA0001793281210000051
Figure BDA0001793281210000061
Figure BDA0001793281210000071
Figure BDA0001793281210000081
Figure BDA0001793281210000091
Figure BDA0001793281210000101
The invention has the following advantages:
8 loci (qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1) related to the major fiber strength genes of upland cotton, wherein the fiber strength synergistic genes of qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1 and qFS-chr20-1 are derived from Xinluzao 24; the fiber strength enhancing genes of qFS-chr20-5 and qFS-c24-1 are from Lu cotton research 28. The 8 major gene loci all show multi-environment stabilization effect and can be used for molecular marker-assisted selection of upland cotton fiber strength. qFS-chr01-2 explained phenotypic variation between 5.32% and 8.86%, additive effect between 0.39-0.55 (cN/Tex); qFS-chr07-1 explained phenotypic variation of 6%, additive effect between 0.49-0.52 (cN/Tex); qFS-chr11-1 explained phenotypic variation between 4.87-5.59%, additive effect between 0.43-0.44 (cN/Tex); qFS-chr11-2 explained phenotypic variation between 5.26% and 7.21%, additive effect between 0.37 and 0.48 (cN/Tex); qFS-chr13-1 explained phenotypic variation between 5.74% and 10.69%, additive effect between 0.48 and 0.72 (cN/Tex); qFS-chr20-1 explained phenotypic variation between 2.91% and 8.18%, additive effect between 0.39-0.67 (cN/Tex); qFS-chr20-5 explained phenotypic variation between 4.5% and 5.9%, additive effect between 0.37 and 0.45 (cN/Tex); qFS-c24-1 explained phenotypic variation between 5.11% and 5.43%, additive effect between 0.40 and 0.51 (cN/Tex).
The invention constructs the genetic linkage map of the recombinant inbred line population through the genotypes of the molecular markers in the population, and identifies the fiber strength phenotype of the population under multi-environment conditions. The genotype and phenotype of the genetic map are combined and analyzed to obtain molecular markers related to fiber strength. The molecular markers closely linked with the fiber strength are utilized to carry out auxiliary selection, so that the fiber strength of the upland cotton variety can be improved in a targeted manner, and the quality breeding efficiency of the upland cotton can be improved.
Drawings
FIG. 1 shows the molecular marker linkage map and major gene locus/QTL of the recombinant inbred line population of the invention. These major gene loci are located on chromosomes 1, 7, 11, 13, 20, and 24, respectively.
Wherein, the markers linked with qFS-chr01-2 are as follows: TM379, TM374, TM378, TM381, TM382, TM386, TM390, TM389, TM391, TM405, TM406, and TM 404; markers linked to qFS-chr07-1 are: TM19848, TM19877, TM19894, TM19872, TM19865, TM19873, TM19870, TM19855, TM19853, TM19859, TM19868, TM19862, and TM 19875; markers linked to qFS-chr11-1 are: TM37826, TM37831, TM37829, and TM 37828; markers linked to qFS-chr11-2 are: TM37897, TM37888, TM37893, TM37880, TM37894, TM37879, TM37878, TM37887, TM37875, TM37883, TM37884, TM37901, TM37904, TM37902, TM37917, TM37906, TM37907, TM37909, TM37908, TM37924, TM37929, TM37912, TM37919, TM37916, TM37918, TM37911, TM37931, TM37938, TM37932, TM37937, and TM 37935; markers linked to qFS-chr13-1 are: TM43230, TM43227, and TM 43229; markers linked to qFS-chr20-1 are: TM75088, TM75098, and TM 75100; markers linked to qFS-chr20-5 are: TM 73152; markers linked to qFS-chr24-1 are: TM67152 and TM 67146.
Detailed Description
The present invention will be further illustrated by the following detailed description of specific embodiments, wherein the molecular markers linked to the major genes of upland cotton fiber strength are obtained by the following methods:
(1) the cultivation method and process of the recombinant inbred line for identifying the fiber strength of upland cotton and constructing the genetic linkage map comprises the following steps: a large-area popularized upland cotton cultivar Lu Cotton research 28 with high yield potential cultivated by Shandong cotton research center is used as a female parent, a high-quality variety Xinluzao 24 cultivated by Xinjiang Kangdi company is used as a male parent, a hybrid combination is configured on a cotton research institute (Henan Anyang) test farm of Chinese academy of agricultural sciences in summer of 2008, and F is planted in Hainan of 20081. Planting 238F plants in Anyang in spring of 20092Individual plants, selfing to obtain F2:3Family members. 231F are selected2:3The family is bred in combination with the additional generation breeding in Hainan in winter, each generation is selfed, each individual plant in the family is selected to be a selfing bell, and the selfing bells are harvested until F2:6At F2:6Randomly selecting a single plant from the family, selfing for 2 generations to obtain F6:8A generation population. F is to be6:8The generation and later populations are used as Recombinant Inbred Line (RIL) populations.
(2) Taking a recombinant inbred line population (F)6：8) The DNA of 231 families and amphiphilic Xinluzao 24 and Lumian 28 were extracted from the leaf samples of (1).
(3) The inbred line population and the parents thereof are subjected to genotype analysis by using a Cotton80KSNP chip, and 4729 SNP markers having polymorphism between two parents and in a recombinant inbred line population are obtained. Simultaneously, 122 SSR markers with polymorphism between two parents and in a recombinant inbred line population are selected. In the invention, the molecular markers related to the major gene sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the cotton fiber strength in upland area are shown in the table 1, and the characteristic sequences, the positions in a physical map and the SNP mutation sites thereof are shown in the table 1.
(4) Genetic linkage maps were constructed using HighMap mapping software (Liu et al, 2014) and the distances between the maps were estimated using Kosambi functions (Kosambi, 1943). A genetic linkage map containing 4851 marker loci (including 4729 SNP markers and 122 SSR markers) is constructed, 26 linkage groups are constructed in total, all the linkage groups are positioned on 26 chromosomes of upland cotton, 2477.99cM is covered on a upland cotton genome, and the average inter-marker distance is 0.51 cM. Wherein subgroup A contains 3300 markers covering the genome 1474.63cM, with an average inter-marker distance of 0.45 cM. Subgroup D contains 1551 molecular markers, covering 1003.36cM genome. The average inter-marker distance was 0.65 cM. The molecular marker linkage map and major gene locus/QTL of the recombinant inbred line population of the invention are shown in figure 1.
(5) Combining the identification result of the fiber quality of the population of the recombinant inbred line, carrying out QTLs positioning by utilizing Windows QTL Cartographer2.5, carrying out 1000 times of sequencing tests, screening the main effect QTLs of the fiber strength stably expressed in multiple environments, and screening 8 main effect fiber strength QTLs, namely: qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c 24-1. Wherein, fiber strength enhancing genes of qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1 and qFS-chr20-1 are derived from Xinluzao 24; the fiber strength enhancing genes of qFS-chr20-5 and qFS-c24-1 are from Lu cotton research 28. Markers linked to qFS-chr01-2 are: TM379, TM374, TM378, TM381, TM382, TM386, TM390, TM389, TM391, TM405, TM406, and TM 404; markers linked to qFS-chr07-1 are: TM19848, TM19877, TM19894, TM19872, TM19865, TM19873, TM19870, TM19855, TM19853, TM19859, TM19868, TM19862, and TM 19875; markers linked to qFS-chr11-1 are: TM37826, TM37831, TM37829, and TM 37828; markers linked to qFS-chr11-2 are: TM37897, TM37888, TM37893, TM37880, TM37894, TM37879, TM37878, TM37887, TM37875, TM37883, TM37884, TM37901, TM37904, TM37902, TM37917, TM37906, TM37907, TM37909, TM37908, TM37924, TM37929, TM37912, TM37919, TM37916, TM37918, TM37911, TM37931, TM37938, TM37932, TM37937, and TM 37935; markers linked to qFS-chr13-1 are: TM43230, TM43227, and TM 43229; markers linked to qFS-chr20-1 are: TM75088, TM75098, and TM 75100; markers linked to qFS-chr20-5 are: TM 73152; markers linked to qFS-chr24-1 are: TM67152 and TM 67146. The 8 major gene loci all show multi-environmental effect stability and can be used for molecular marker-assisted selection for improving the quality of upland cotton fibers. qFS-chr01-2 explained phenotypic variation between 5.32% and 8.86%, additive effect between 0.39-0.55 (cN/Tex); qFS-chr07-1 explained phenotypic variation of 6%, additive effect between 0.49-0.52 (cN/Tex); qFS-chr11-1 explained phenotypic variation between 4.87-5.59%, additive effect between 0.43-0.44 (cN/Tex); qFS-chr11-2 explained phenotypic variation between 5.26% and 7.21%, additive effect between 0.37 and 0.48 (cN/Tex); qFS-chr13-1 explained phenotypic variation between 5.74% and 10.69%, additive effect between 0.48 and 0.72 (cN/Tex); qFS-chr20-1 explained phenotypic variation between 2.91% and 8.18%, additive effect between 0.39-0.67 (cN/Tex); qFS-chr20-5 explained phenotypic variation between 4.5% and 5.9%, additive effect between 0.37 and 0.45 (cN/Tex); qFS-c24-1 explained phenotypic variation between 5.11% and 5.43%, additive effect between 0.40 and 0.51 (cN/Tex).

Claims (2)

1. The application of molecular markers closely linked with major gene sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the cotton fiber strength in cotton auxiliary breeding to improve the cotton fiber strength, the method is characterized in that the major gene sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the fiber strength of upland cotton are respectively and sequentially located on chromosomes 1, 7, 11, 13, 20 and 24 of upland cotton; the marker linked to qFS-chr01-2 is TM 379-TM 404; the marker linked to qFS-chr07-1 is TM 19848-TM 19875; the marker linked to qFS-chr11-1 is TM 37826-TM 37828; the marker linked to qFS-chr11-2 is TM 37897-TM 37935; the marker linked to qFS-chr13-1 is TM 43230-TM 43229; the marker linked with qFS-chr20-1 is TM 75088-TM 75100; the marker linked to qFS-chr20-5 is TM 73152; the marker linked to qFS-c24-1 is TM 67152-TM 67146; the molecular markers related to the major gene sites qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 of the cotton fiber strength in upland area are characterized in that the characteristic sequences, the positions in a physical map and SNP mutation sites are shown in the following table one:
table one: position of qFS on genetic map and physical map, SNP flanking sequence and parent genotype
Figure FDA0002970245740000011
Figure FDA0002970245740000021
Figure FDA0002970245740000031
Figure FDA0002970245740000041
Figure FDA0002970245740000051
Figure FDA0002970245740000061
Figure FDA0002970245740000071
2. The use of claim 1, wherein the molecular markers closely linked to the major gene loci of upland cotton fiber strength qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c24-1 are obtained by:
1) the method comprises the steps of preparing a hybrid combination by using a high-yield upland cotton high-yield cultivation variety Lu-Cotton research 28 popularized in large area in cotton production as a female parent and a high-quality variety Xinluzao 24 of upland cotton as a male parent, and breeding a recombinant inbred line group containing 231 inbred lines on the basis of the hybrid combination;
2) carrying out genotype analysis on the recombinant inbred line population and the parents thereof by using a upland cotton SNP80K chip to obtain 4729 SNP markers with polymorphism between two parents and between the recombinant inbred lines in the recombinant inbred line population; simultaneously, polymorphism analysis is carried out on the two parents by using SSR markers, genotyping is carried out on the recombinant inbred line population by using the SSR markers with polymorphism between the two parents to obtain 122 SSR markers in total, and a genetic linkage map containing 4851 markers and with the total map distance of 2477.99cM is constructed;
3) under the natural production condition of the field, performing multi-environment phenotype identification on the fiber strength of the recombinant inbred line population in two ecological areas of Xinjiang and yellow river watershed;
4) according to the fiber strength phenotype identification result of the recombinant inbred line population under the natural production condition of the field, the genetic linkage map containing 4851 markers is utilized to carry out major QTL positioning of the fiber strength of the upland cotton in multiple environments, and 8 QTLs which are stable in multiple environments and related to the fiber strength of the upland cotton are positioned in total, namely: qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1, qFS-chr20-1, qFS-chr20-5 and qFS-c 24-1; wherein, fiber strength enhancing genes of qFS-chr01-2, qFS-chr07-1, qFS-chr11-1, qFS-chr11-2, qFS-chr13-1 and qFS-chr20-1 are derived from Xinluzao 24; fiber strength enhancing genes of qFS-chr20-5 and qFS-c24-1 are from Lu cotton research 28; markers linked to qFS-chr01-2 are: TM379, TM374, TM378, TM381, TM382, TM386, TM390, TM389, TM391, TM405, TM406, and TM 404; markers linked to qFS-chr07-1 are: TM19848, TM19877, TM19894, TM19872, TM19865, TM19873, TM19870, TM19855, TM19853, TM19859, TM19868, TM19862, and TM 19875; markers linked to qFS-chr11-1 are: TM37826, TM37831, TM37829, and TM 37828; markers linked to qFS-chr11-2 are: TM37897, TM37888, TM37893, TM37880, TM37894, TM37879, TM37878, TM37887, TM37875, TM37883, TM37884, TM37901, TM37904, TM37902, TM37917, TM37906, TM37907, TM37909, TM37908, TM37924, TM37929, TM37912, TM37919, TM37916, TM37918, TM37911, TM37931, TM37938, TM37932, TM37937, and TM 37935; markers linked to qFS-chr13-1 are: TM43230, TM43227, and TM 43229; markers linked to qFS-chr20-1 are: TM75088, TM75098, and TM 75100; markers linked to qFS-chr20-5 are: TM 73152; markers linked to qFS-chr24-1 are: TM67152 and TM 67146.
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