CN1774998A - BTtype Yunnan-type cytoplasmic breeded indica sterile line method - Google Patents
BTtype Yunnan-type cytoplasmic breeded indica sterile line method Download PDFInfo
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- CN1774998A CN1774998A CN 200510061727 CN200510061727A CN1774998A CN 1774998 A CN1774998 A CN 1774998A CN 200510061727 CN200510061727 CN 200510061727 CN 200510061727 A CN200510061727 A CN 200510061727A CN 1774998 A CN1774998 A CN 1774998A
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Abstract
The present invention discloses a method for cultivating long grain non-glutinous rice sterile line by using cytoplasm of BT type and Yunnan type. It uses early-maturing broad-affinity chunjiang 05 keng rice maintenance line material as female parent and uses low-stemmed early-maturing high-quality and disease-resistant long-grain non-glutinous rice maintenance as male parent, and respectively adopts the processes of hybridization, back-crossing and transfer cultivation so as to obtain the invented long-grain non-glutinous rice sterile line. Said invention also provides the concrete steps of every above-mentioned process.
Description
Technical field
The invention belongs to the rice breeding field, particularly the utilization of paddy rice inter-subspecific heterosis.
Background technology
Paddy rice is the first cereal crops of China, and its cultivated area accounts for 30% of national cereal crops cultivated area, and output accounts for 40% of total output of grain.The hybrid rice of whole nation plantation now area accounts for 50% of rice area, and output then is about 60% of paddy rice gross yield.The paddy rice cross breeding advantage is generally acknowledged, than conventional rice volume increase about 10%, is that another important milestone makes China's rice yield occur twice and increases production greatly, develops on a large scale after the short barization of rice varieties at the end of the fifties in last century to the beginning of the sixties generally.China's breeding of hybridized rice constantly makes progress, still keep world lead level at present, but along with the adjustment of the structure of rural undertaking, arable area reduces constantly, population constantly increases, and grain security, growth of agricultural efficiency, increasing peasant income have become the problem that governments at all levels extremely pay close attention to.
Paddy rice is the same with other crop, and heterotic power is limited by the genetic variation and genetic differentiation level of parental gene.The hybrid rice of China's popularization at present because the physiological disorder of indica rice subspecies all can only be utilized with the hybrid vigour in a kind of, has restricted the potentiality of further raising output.Powerful hybrid vigour between indica and japonica subspecies is that early scientists payes attention to, but fertility length that Hybrids of Indica and Japonica exists, solid rate variance, substantial physiology, reproductive disorder such as not full have influenced heterotic utilization.The rice breeding worker has done a large amount of research in this respect, has obtained significant achievement.Utilize the part indica-japonica heterosis, cultivated a collection of super indica hybrid rice.But owing to be subjected to the restriction in the cytoplasm source of long-grained nonglutinous rice male sterile line, the flower pesticide abortion of long-grained nonglutinous rice male sterile line is that allusion quotation loses, exist recovery face narrow and numerous kind, spend problem such as time difference during the production of hybrid seeds, restricted heterotic performance, and numerous kind, the further raising of hybrid seed yield.
Summary of the invention
The objective of the invention is to mingle the problems referred to above of paddy rice, and provide the cytoplasm of a kind of BT type, Yunnan type to breed the method for indica cms line for solving the Xian round-grained rice.The male sterile line that this method is cultivated is that a kind of recovery is wide, high-combining ability, super high-yielding, high-quality, adaptability strong, numerous kind, hybrid seed yield height, part is utilized the breeding of hybridized rice new material of indica-japonica heterosis, is suitably for to cultivate the super high-yielding combination for producing upward large-area applications.
Paddy rice " three line method " heterosis utilization is the plasmotype sterility, and is different because of the cytoplasm source mainly owing to cytoplasm and cell nucleus is inharmonious causes, just produces flower pesticide abortion degree difference.The cytoplasm majority of long-grained nonglutinous rice male sterile line derives from the open country and loses, and the flower pesticide abortion is that allusion quotation loses.The cytoplasm of japonica rice sterile (BT type, Yunnan type) derives from long-grained nonglutinous rice, and the flower pesticide abortion is that the garden is lost, dyed and lose.Breed the cytoplasmic paddy rice indica cms line of BT type, Yunnan type, will be from improvement long-grained nonglutinous rice maintenance line, selection has the japonica rice maintenance line of wide affinity characteristic and has short bar high-quality, disease-resistant long-grained nonglutinous rice maintenance line material, with wide compatibility gene and the transformation of japonica rice sterile gene to good indica cms line.
Based on above reason and thinking, the BT type that the present invention provides for achieving the above object, the cytoplasm of Yunnan type are bred the method for indica cms line, it is characterized in that its concrete steps are as follows:
A, selection have the japonica rice maintenance line material of wide compatibility gene for maternal, are male parent with short bar, high-quality, disease-resistant long-grained nonglutinous rice maintenance line material, hybridize, and it hybridizes F
1Dai Zaiyong long-grained nonglutinous rice maintenance line is backcrossed;
B, through selecting the ideotype of inclined to one side Xian in the middle generation of backcrossing, promptly ripe phase, good, the solid normal indica type rice plant of disease resistance carry out transformation with the sterile line material of BT type or Yunnan type;
C, be in the cytoplasmic transformation sub-district in BT type, Yunnan type, select high sub-district of complete sterility strain or sterile plant rate and corresponding indica type maintenance line material thereof, and to meet the breeding objective requirement as the transformation emphasis, carry out continuous transformation and backcross, stable until the indica type maintenance line material settling out and the sterility of transformation improvement;
The inventive method also comprises steps d: to stable sterile material, recover the face test cross, match strong excellent hybrid rice combination.
Described BT type is spring river 05A etc.
Described Yunnan type is N27A or N117A etc.
The described maternal material of japonica rice maintenance line with wide compatibility gene is spring river 05 etc.
Described short bar, high-quality, disease-resistant long-grained nonglutinous rice maintenance line male parent material are that early Xian 312 improves material N2770, N2742 etc.
The feature of the BT type of having bred, the indica cms line material of Yunnan type:
Short bar, plant height are at 75～85 centimetres, plant is neat, and tillering ability is strong, and average every fringe is about 100, it is about 55～60 days that work rice in local dry season is broadcast phase beginning, flowering habit is good, the stigma exposing ratio height, and the flower pesticide abortion is that the garden is lost, dyed and lose, help improving numerous kind, hybrid seed yield, join through pacing just, the round-grained rice type of the indica type recovery material of round-grained rice matter and round-grained rice matter is recovered material all recovery capability, recovers characteristic such as wide.
Compared with prior art, advantage of the present invention is that the male sterile line of breeding has following characteristics:
1, recovers wide
The indica cms line that the present invention breeds through preliminary test cross, all has recovery capability to the round-grained rice type recovery system of round-grained rice matter and the indica restorer line of round-grained rice matter, has strong hybrid vigour, helps matching out strong advantage combination.
2, numerous kind and hybrid seed yield height
The BT type of breeding, the indica cms line of Yunnan type, flowering habit is good, and the flower pesticide abortion is that the garden is lost, dyed and lose, and the stigma exposing ratio height, helps further improving the output of the breeding seed and the production of hybrid seeds.
Description of drawings
The cultivating process schematic diagram of Fig. 1 embodiment of the invention.
Specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment
Shown in the accompanying drawing 1, be the cultivating process schematic diagram of present embodiment.Concrete cultivating process is:
Season morning in 2003, the japonica rice maintenance line material of wide compatibility gene---spring river 05 was a female parent to have in Ningbo, with short bar, high-quality, disease-resistant long-grained nonglutinous rice maintenance line material---and early Xian 312 improvement material N2770 or N2742 are male parent, hybridize;
Season in evening in 2003 was selected above-mentioned hybridization F in Ningbo
1Dai Zaiyong N2770 or N2742 backcross;
Winter～2004 spring in 2003 is in Hainan, and through selecting the ideotype of inclined to one side Xian in the middle generation of backcrossing, i.e. ripe phase, good, the solid normal indica type rice plant of disease resistance carry out transformation with the sterile line material N27A or the N117A of BT type spring river 05A or Yunnan type;
After this, from year season in evening in season to 2005 morning in 2004, wherein season morning, season in evening are in Ningbo, the Winter-Spring is in Hainan, in BT type, Yunnan type is in the cytoplasmic transformation sub-district, select complete sterility strain or high sub-district and the corresponding indica type maintenance line material thereof of sterile plant rate, and to meet the breeding objective requirement as the transformation emphasis, carrying out continuous transformation backcrosses, early promptly obtained basicly stable maintenance line material and sterile line material after season to 2005, season in evening in 2005 gained maintenance line material and the stability of sterile line material more satisfactory.In addition, spring in 2005 also to stable sterile material, was carried out recovery face test cross in Hainan.
The feature of the BT type of having bred, the indica cms line material of Yunnan type:
Short bar, plant height are at 75～85 centimetres, plant is neat, and tillering ability is strong, and average every fringe is about 100, it is about 55～60 days that work rice in local dry season is broadcast phase beginning, flowering habit is good, the stigma exposing ratio height, and the flower pesticide abortion is that the garden is lost, dyed and lose, help improving numerous kind, hybrid seed yield, join through pacing just, the indica type recovery material of round-grained rice matter and the round-grained rice type recovery material of round-grained rice matter are all had recovery capability, recover wide and numerous kind and hybrid seed yield height.
Claims (7)
1, the cytoplasm of BT type, Yunnan type is bred the method for indica cms line, it is characterized in that its concrete steps are as follows:
A, selection have the japonica rice maintenance line material of wide compatibility gene for maternal, are male parent with short bar, high-quality, disease-resistant long-grained nonglutinous rice maintenance line material, hybridize, and it hybridizes F
1Dai Zaiyong long-grained nonglutinous rice maintenance line is backcrossed;
B, through selecting the ideotype of inclined to one side Xian in the middle generation of backcrossing, promptly ripe phase, good, the solid normal indica type rice plant of disease resistance carry out transformation with the sterile line material of BT type or Yunnan type;
C, be in the cytoplasmic transformation sub-district in BT type, Yunnan type, select high sub-district of complete sterility strain or sterile plant rate and corresponding indica type maintenance line material thereof, and to meet the breeding objective requirement as the transformation emphasis, carry out continuous transformation and backcross, stable until the indica type maintenance line material settling out and the sterility of transformation improvement;
2, method according to claim 1 is characterized in that further comprising the steps of:
D, to stable sterile material, recover the face test cross, match strong excellent hybrid rice combination.
3, method according to claim 1 and 2 is characterized in that described BT type is spring river 05A.
4, method according to claim 1 and 2 is characterized in that described Yunnan type is N27A or N117A.
5, method according to claim 1 and 2 is characterized in that the described maternal material of japonica rice maintenance line with wide compatibility gene is spring river 05.
6, method according to claim 1 and 2 is characterized in that described short bar, high-quality, disease-resistant long-grained nonglutinous rice maintenance line male parent material are that early Xian 312 improves materials.
7, method according to claim 6 is characterized in that described morning, Xian 312 improvement materials were N2770, N2742.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200510061727 CN1774998A (en) | 2005-11-25 | 2005-11-25 | BTtype Yunnan-type cytoplasmic breeded indica sterile line method |
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| CN 200510061727 CN1774998A (en) | 2005-11-25 | 2005-11-25 | BTtype Yunnan-type cytoplasmic breeded indica sterile line method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599048A (en) * | 2012-03-28 | 2012-07-25 | 云南农业大学 | Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding |
| CN104756861A (en) * | 2015-04-02 | 2015-07-08 | 上海市农业科学院 | Method for breeding early maturing japonica rice |
| CN104839012A (en) * | 2015-06-04 | 2015-08-19 | 张友恒 | Method for breeding wide-compatibility indica male sterile line with dian-type cytoplasm |
| CN106962185A (en) * | 2017-05-26 | 2017-07-21 | 宁波市农业科学研究院 | It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance |
| CN111631129A (en) * | 2020-05-26 | 2020-09-08 | 上海市农业科学院 | Rapid breeding method of fragrant and soft hybrid japonica rice sterile line |
-
2005
- 2005-11-25 CN CN 200510061727 patent/CN1774998A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599048A (en) * | 2012-03-28 | 2012-07-25 | 云南农业大学 | Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding |
| CN104756861A (en) * | 2015-04-02 | 2015-07-08 | 上海市农业科学院 | Method for breeding early maturing japonica rice |
| CN104839012A (en) * | 2015-06-04 | 2015-08-19 | 张友恒 | Method for breeding wide-compatibility indica male sterile line with dian-type cytoplasm |
| CN106962185A (en) * | 2017-05-26 | 2017-07-21 | 宁波市农业科学研究院 | It is a kind of while improving the breeding method of conventional japonica rice and round-grained rice type two-line sterile line rice blast resistance |
| CN111631129A (en) * | 2020-05-26 | 2020-09-08 | 上海市农业科学院 | Rapid breeding method of fragrant and soft hybrid japonica rice sterile line |
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