CN1378777A - Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization - Google Patents
Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization Download PDFInfo
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- CN1378777A CN1378777A CN 02113659 CN02113659A CN1378777A CN 1378777 A CN1378777 A CN 1378777A CN 02113659 CN02113659 CN 02113659 CN 02113659 A CN02113659 A CN 02113659A CN 1378777 A CN1378777 A CN 1378777A
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Abstract
A bee pollination method for reproducing and hybridizing the nuclear male sterility line of cotton features that the insect-resistant nuclear male sterility line is used as the female parent and bee is used for pollination, and no pesticide is applied in the pollination period. Its advantages are saving babour and time, low cost and high yield of cotton.
Description
Affiliated technical field
The present invention relates to grow cotton a sterile line propagation and a cross-breeding method.
Background technology
Utilizing hybrid vigour is the effective way that increases substantially crop yield and improvement quality, but the utilization of cotton hybrid vigor lags behind other crop all the time.Main cause is that cotton is normal cross pollinated plant.In the ordinary course of things natural hybrization rate only is 5-10%, so the cotton hybrid production of hybrid seeds, necessary by extraneous supple-mentary pollination.Widely used now kernel male sterile two line method, kernel male sterile secondary method and manual detasseling method preparing hybrid kind all need artificial pollination.Because cotton is unlimited titbit, bloom and the artificial pollination time long, workload is big, the labor cost height, the rural laborer goes out morely in recent years in addition, more limits, hinders the production of cotton hybrid seed and popularization and the utilization of cenospecies.So seek simpler and more direct producing method for seed, and reduce labour's input that the cotton hybrid seed is produced, be a difficult problem and the developing direction that the crossbreeding urgent need is captured.
Summary of the invention
Purpose of the present invention just provides and does not a kind ofly need the artificial pollination can breeding male sterile lines and hybrid seeding, the saving of labor, save time, labour's less investment, cost is low, the production of hybrid seeds is profitable cotton nuclear male sterile line honeybee pollination breeding and cross-breeding method.
The technical solution used in the present invention is for achieving the above object: a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding method, with " one is two usages (GMS methods) " breeding genic male sterility dual purpose and hybrid seeding, perhaps use " secondary method (MM method) " hybrid seeding, its characteristics are: maternal is pest-resistant genic male sterile line, the breeding of every mu of flowering and boll-setting period or seed farm are placed 1-2 railway carriage or compartment honeybee and are pollinated, applying pesticides not during the honey bees pollination.
In the above-mentioned a kind of cotton nuclear male sterile line honeybee pollination breeding and cross-breeding method: the method with " one is two usages (GMS methods) " breeding genic male sterility dual purpose is: the pest-resistant genic male sterility dual purpose cotton of plantation in the breeding field, flowering and boll-setting period carries out mark to sterile strain, collects seed in the sterile strain of mark as using genic male sterility dual purpose next year of breeding.
In the above-mentioned a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding method: the method with " one is two usages (GMS methods) " hybrid seeding is:
The female parent district that a, every row male parent and 3-6 are capable is alternate plants in same seed farm, cotton variety, strain that the plantation of male parent row is good, the pest-resistant genic male sterility dual purpose cotton of maternal district's plantation;
The identification of b, flower bud phase is also in time pulled out educated strain in the maternal district, substantially pull out to the greatest extent after, put honeybee pollination, put behind the honeybee in 20 days, before the every morning cotton loose powder, by strain identification and pull out blooming in the maternal district and can educate strain.
Pest-resistant genic male sterility dual purpose cotton is the anti-A of pest-resistant kernel sterility dual serial
3
Above-mentioned a kind of cotton nuclear male sterile line honeybee pollinates in breeding and the cross-breeding method: use the method for " secondary method (MM method) " hybrid seeding to be: with the pest-resistant perfect kernel male sterile line body female parent that cell nucleus key-gene-multiple gene is controlled jointly, the capable female parent of every 3-6 and 1 is gone the alternate kind of male parent in same seed farm.
Sterile with the pest-resistant perfect kernel of nucleus key-gene-polygenes co-controlling is pest-resistant perfect kernel sterile line MA
2
In the above-mentioned a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding method: do not plant other cotton material in 1 kilometer around breeding and the seed farm; Stop pollination 20-25 day in August.
The utility model adopts technical scheme as above, its beneficial effect is: when the breeding and the production of hybrid seeds, need not carry out artificial pollination, and adopt honeybee pollination, save trouble, save time, cost is low, reduces labour's input that cotton crossbreed is produced significantly, adapts to the needs of the current structural readjustment of rural industry and Labor Transfer, help the cotton crossbreed utilization and extention, improve output of cotton, additional income has good economic benefits and social benefit.
Embodiment
The present invention is described in further detail below by embodiment:
Embodiment one: be two usages (GMS method) " breeding kernel sterility dual serial; the pest-resistant genic male sterility dual purpose cotton of plantation in the breeding field; flowering and boll-setting period carries out mark to sterile strain (accounting for 50%); place 1～2 railway carriage or compartment honeybee in the inherent every mu of breeding field of flowering and boll-setting period simultaneously and carry out honey bees pollination, applying pesticides no longer during the placement honeybee with one.The unlikely agricultural chemicals that is subjected to of honeybee is injured, and is pest-resistant cotton owing to what plant in the field simultaneously, and cotton can insect infestation.20-25 day in August, the breeding field is taken honeybee away, is stopped pollination (pollination).Do not plant the cotton of other strain during plantation around the breeding field in one kilometer.When receiving flower, collect the upper seed of mark as production of hybrid seeds next year or the stock genic male sterility dual purpose of breeding.Unmarked or make in addition seed in the educated strain of isolabeling not and then do not make kind of usefulness and process by the commodity cottonseed.
In this example, accounting for 50% sterile group of hill body in the dual-purpose system, to serve as sterile line namely maternal, and to serve as maintainer be male parent to about 50% educated group of hill body in addition.
Embodiment two: be two usages (GMS method) hybrid seedings with one: the female parent district of every row male parent and 3～6 row is alternate plants in same production of hybrid seeds field, the cotton variety that the plantation of male parent row is good, strain, the pest-resistant genic male sterility dual purpose cotton of maternal district's plantation.Flower bud phase identification is also in time pulled out educated strain in the maternal district, substantially pull out to the greatest extent (90% educated strain is pulled out) after, place 1-2 railway carriage or compartment honeybee in every mu of seed farm and pollinate, put between the honeybee pollinating period, not applying pesticides.Put simultaneously behind the honeybee in 20 days, before the every morning cotton loose powder, by strain identification and pull out blooming in the maternal district and can educate strain.Certainly, if can be sure of does not have to educate strain in the maternal district fully, then need not check full 20 days, checks more than 10 days but generally should discern.August 20～25 was taken honeybee away, stops pollination from seed farm, collected the seed in the maternal district when receiving flower, the cenospecies for use next year that the male parent that is merit and pest-resistant genic male sterility dual purpose hybridization of female parent produce.Do not plant other cotton material in 1 kilometer around the seed farm yet.
In this example, the maternal sterile group of hill body of not pulling out and being used of distinguishing serves as the i.e. female parent of sterile line.
Embodiment three: use secondary method (MM method) hybrid seeding:
With the nucleus key-gene---the pest-resistant perfect kernel sterile line of polygenes co-controlling is made female parent, and the maternal alternate kind with 1 row male parent of per 3～6 row is in same seed farm.Because female parent is complete sterile line, its sterile plant rate reaches 100%, and flower colony sterile degree reaches 95-100%, so do not need to carry out the work of pulling out that strain can be educated by maternal district again.Male parent (recovering system) can be all common upland cotton and sea-island cotton.See spend after, every mu of seed farm is placed 1-2 railway carriage or compartment honeybee and is pollinated, and puts not applying pesticides of honeybee pollinating period.Took honeybee away on August 20～25, stop pollination, collect the seed that the perfect kernel male sterile line is set seeds and produced, it promptly is the crossbreed that good male parent and pest-resistant perfect kernel male sterile line hybridization of female parent produce for large tracts of land use next year, the same with embodiment one, two, do not plant other cotton material around the seed farm in one kilometer yet.
Among the embodiment one, two, pest-resistant genic male sterility dual purpose cotton can be adopted the anti-A of pest-resistant kernel sterility dual serial of the applicant's seed selection
3In this moment example two, every row male parent and 4 row are maternal distinguish alternate plant in the effect of same seed farm best.Among the embodiment three, with the nucleus key-gene---the pest-resistant perfect kernel sterile line of polygenes co-controlling can adopt the pest-resistant perfect kernel sterile line MA of the applicant's seed selection
2The maternal alternate kind with 1 row male parent of per 4 row this moment is in the best results of same seed farm.Certainly also can adopt other pest-resistant genic male sterility dual purpose or pest-resistant perfect kernel male sterile line, contain hole A type sterile gene ms as all
14Sterile material pest-resistant genic male sterility dual purpose or the pest-resistant perfect kernel male sterile line bred, as long as can satisfy between pollinating period not applying pesticides, maternal cotton does not produce insect pest, the requirement that other proterties also meets breeding, the production of hybrid seeds gets final product.
The male parent of using among the present invention does not require it must is pest-resistant.Because the male parent cotton only provides pollen, required plant few, and the breeding of dual-purpose system is all only collected the seed of setting seeds on the maternal cotton and producing with hybrid seeding.So at the flowering and boll-setting period no pesticide grown, whether insect infestation is unimportant for the male parent cotton.
Claims (7)
1, a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding method, with " one is two usages (GMS methods) " breeding genic male sterility dual purpose and hybrid seeding, perhaps use " secondary method (MM method) " hybrid seeding, it is characterized in that: maternal is pest-resistant genic male sterile line, the breeding of every mu of flowering and boll-setting period or seed farm are placed 1-2 railway carriage or compartment honeybee and are pollinated, applying pesticides not during the honey bees pollination.
2, a kind of cotton nuclear male sterile line honeybee pollination breeding according to claim 1 and cross-breeding method, it is characterized in that: the method for described usefulness " one is two usages (GMS methods) " breeding genic male sterility dual purpose is: the pest-resistant genic male sterility dual purpose cotton of plantation in the breeding field, flowering and boll-setting period carries out mark to sterile strain, collects seed in the sterile strain of mark as using genic male sterility dual purpose next year of breeding.
3, a kind of cotton nuclear male sterile line honeybee pollination breeding according to claim 1 and cross-breeding method, it is characterized in that: the method for described usefulness " one is two usages (GMS methods) " hybrid seeding is:
The female parent district of a, every row male parent and 3～6 row is alternate plants in same seed farm, cotton variety, strain that the plantation of male parent row is good, the pest-resistant genic male sterility dual purpose cotton of maternal district's plantation;
The identification of b, flower bud phase is also in time pulled out educated strain in the maternal district, substantially pull out to the greatest extent after, put honeybee pollination, put behind the honeybee in 20 days, before the every morning cotton loose powder, by strain identification and pull out blooming in the maternal district and can educate strain.
4, according to claim 2 or 3 described a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding methods, it is characterized in that: described pest-resistant genic male sterility dual purpose cotton is the anti-A of pest-resistant kernel sterility dual serial
3
5, a kind of cotton nuclear male sterile line honeybee pollination breeding according to claim 1 and cross-breeding method, it is characterized in that: the method for described usefulness " secondary method (MM method) " hybrid seeding is: sterile with the common pest-resistant perfect kernel of controlling of cell nucleus key-gene-multiple gene is maternal, and capable female parent of every 3-6 and the alternate kind of 1 row male parent are in same seed farm.
6, a kind of cotton nuclear male sterile line honey bees pollination breeding according to claim 5 and cross-breeding method, it is characterized in that: sterile with the pest-resistant perfect kernel of nucleus key-gene-polygenes co-controlling is pest-resistant perfect kernel sterile line MA
2
7, according to claim 2 or 3 or 5 or 6 described a kind of cotton nuclear male sterile line honey bees pollination breeding and cross-breeding methods, it is characterized in that: do not plant other cotton material in 1 kilometer around breeding and the seed farm; Stop pollination 20-25 day in August.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 02113659 CN1378777A (en) | 2002-04-26 | 2002-04-26 | Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization |
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| CN 02113659 CN1378777A (en) | 2002-04-26 | 2002-04-26 | Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107916B (en) * | 2007-08-02 | 2010-04-07 | 四川诺亚生物科技有限公司 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
| CN101124885B (en) * | 2007-08-24 | 2011-03-23 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
| CN101124886B (en) * | 2007-08-24 | 2011-03-23 | 四川省农业科学院经济作物育种栽培研究所 | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration |
| CN104255435A (en) * | 2014-09-24 | 2015-01-07 | 西北农林科技大学 | Breeding method of semi-okra leaf type cotton variety |
| CN109349098A (en) * | 2018-11-08 | 2019-02-19 | 长江大学 | A kind of cotton hybrid seed production method |
| CN111972281A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method of cotton nuclear sterile dual-purpose line |
| CN111972282A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Production method of cotton genic male sterile dual-purpose line hybrid seeds |
-
2002
- 2002-04-26 CN CN 02113659 patent/CN1378777A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107916B (en) * | 2007-08-02 | 2010-04-07 | 四川诺亚生物科技有限公司 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
| CN101124885B (en) * | 2007-08-24 | 2011-03-23 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
| CN101124886B (en) * | 2007-08-24 | 2011-03-23 | 四川省农业科学院经济作物育种栽培研究所 | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration |
| CN104255435A (en) * | 2014-09-24 | 2015-01-07 | 西北农林科技大学 | Breeding method of semi-okra leaf type cotton variety |
| CN109349098A (en) * | 2018-11-08 | 2019-02-19 | 长江大学 | A kind of cotton hybrid seed production method |
| CN111972281A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Breeding method of cotton nuclear sterile dual-purpose line |
| CN111972282A (en) * | 2020-08-27 | 2020-11-24 | 四川省农业科学院经济作物育种栽培研究所 | Production method of cotton genic male sterile dual-purpose line hybrid seeds |
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