CN101107916B - Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root - Google Patents
Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root Download PDFInfo
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- CN101107916B CN101107916B CN2007100496562A CN200710049656A CN101107916B CN 101107916 B CN101107916 B CN 101107916B CN 2007100496562 A CN2007100496562 A CN 2007100496562A CN 200710049656 A CN200710049656 A CN 200710049656A CN 101107916 B CN101107916 B CN 101107916B
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Abstract
The invention discloses an asexual propagation method for nuclear male sterile line, restores line and common line in cotton by utilizing perennial root. The course of action is that: planting cottonof nuclear male sterile line, restores line and common line in the area that the cotton is capable of living through the winter for the first year and reserving the cotton plant to pass the winter naturally after harvested the seeds; then in the second year or each year later, the cotton plant with perennial root of the previous year gives off lateral bud and grows out new plantlet by the asexualpropagation. Harvesting seeds on the plantlet features asexual propagation to realize the asexual propagation for the parents of cotton nuclear coenospecies and cotton line under the conditions of perennial root of high purity strain, so as to maintain the reliability of netic gene and reduce the gene recombination and genovariation due to sexual multiplication; meanwhile, in cooperation with species pollinated by insects during propagating sterile line, the invention can both maintain the hereditary purity and lower the production cost.
Description
Affiliated field
The present invention relates to a kind of asexual reproduction method that utilizes perennial root to carry out cotton kernel sterility dual serial, recovery system and common strain.
Background technology
Cotton is an often cross-pollinated crop, and outcrossing rate is 3-10% generally speaking, can be up to 50% under the more situation of insect pollinator.In addition, self there is certain genetic heterozygosis in its parents of hybrid combination of seed selection at present, even isolate the selfing of bolt flower fully, its filial generation and parental generation also can morph because of genetic recombination.
People are in breeding parent's process, always according to selecting the excellent bad method of eliminating, if logical in a few years the breeding of isolation fully, all to isozygoty when its parents may be than combination selection or good, but because parents' change, the specific combining ability of its combined hybrid advantage also changes thereupon; If logical in a few years non-the isolation fully breeds, then the existing different friendship of parents has self reorganization again, and the variation of its parent and offspring's combination is bigger.
Therefore, after the good hybrid combination of seed selection of breeding man or good common cotton strain, logical experiment and demonstration in a few years, treat real expansion when promoting, change has taken place when hybrid after its parents and the combination or the common high-quality cotton strain of being released and initial test, some is changed beyond recognition, because breeding man also can't preserve the seed selection parents or the common strain in when success fully.Thereby the male parent and the female parent of good cotton strain of the breeding of not having variation or cotton hybrid combination keep the stability of cotton strain or hybrid combination, are problems anxious to be solved.
Summary of the invention
First purpose of the present invention just provides a kind of asexual reproduction method that utilizes perennial root to carry out the cotton kernel sterility dual serial, the offspring of the cotton kernel sterility dual serial that this kind method breeds does not produce genetic variation and reorganization, can reliably keep hybridizing maternal stability, make the quality and yield of hybrid cotton can access reliable assurance.And, saving of work and time, expense is low, helps commercialization and promotes.
The present invention solves the technical scheme that this goal of the invention adopts: utilize perennial root to carry out the asexual reproduction method of cotton kernel sterility dual serial, the steps include:
A, minimum temperature is greater than 10 ℃ area in the winter time,, the plant cotton kernel sterility dual serial, the identification of flower bud phase can be educated strain and sterile strain, pulls out in the cotton field 4/5～3/5 educated strain, and mark is done in remaining 1/5～2/5 educated strain; Bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell in the sterile strain of results uses for the preparation cotton crossbreed;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, when the regrowth that sends in the cotton stake that beta pruning is stayed reaches the 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, bloom the knot bell phase, utilize insect pollination or artificial pollination, and the educated strain that stay last year is made marks again, seed behind the knot bell in the sterile strain of results uses for the preparation cotton crossbreed;
D, repetition B, C step 1～3 time.
Second purpose of the present invention just provides a kind of asexual reproduction method that utilizes perennial root to carry out cotton nucleic sterility restoring line and common strain, the restoring line of cotton that this kind method breeds and the offspring of common strain do not produce genetic variation and reorganization, can reliably keep hybridizing the stability of male parent and common cotton strain, make the quality and yield of hybrid cotton and common cotton can access reliable assurance.And, saving of work and time, expense is low, helps commercialization and promotes.
The present invention solves the technical scheme that this goal of the invention adopts: utilize perennial root to carry out the asexual reproduction method of cotton nucleic sterility restoring line or common cotton strain, the steps include:
A, minimum temperature is greater than 10 ℃ area in the winter time,, plant cotton nucleic sterility restoring line or common cotton strain are bloomed the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, when the regrowth that sends in the cotton stake that beta pruning is stayed reaches the 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant is for using;
D, repetition B, C step 1～3 time.
Compared with prior art, the invention has the beneficial effects as follows: adopt once sowing, 3～5 years perennial roots of surviving the winter, on the perennial root cotton continuously during the pit-sterility cross-bred production of hybrid seeds of cotton in 3～5 years of results maternal seed of dual-purpose system and recovery be male parent seed and common strain, by vegetative mode, breeding results continuously cotton parent and the common strain seed in 3～5 years, thereby fundamentally avoided existing selfing, different friendship, the genetic recombination that hybridization waits the sexual propagation mode to be brought, variation, guaranteed the genetic high stability of cotton hybrid parent and common strain, made the quality and yield of hybrid cotton and common cotton can access reliable assurance.And once sowing, results have reduced the labour's input and the seed expense of sowing every year and have used for many years; Be aided with insect pollination again, reduce the workload of artificial pollination, make saving of work and time of the present invention, expense is low, helps commercialization and promotes.
The present invention is described in further detail below in conjunction with concrete embodiment.
Embodiment
Embodiment one
The present invention utilizes perennial root to carry out the asexual reproduction method of cotton kernel sterility dual serial, the steps include:
A, in the production of hybrid seeds district that cotton plants can be survived the winter winter minimum temperature greater than 10 ℃ area, the plant cotton kernel sterility dual serial, the identification of flower bud phase can be educated strain and sterile strain, pulls out in the cotton field 4/5～3/5 educated strain, and mark is done in remaining 1/5～2/5 educated strain; Bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell in the sterile strain of results uses for the preparation cotton crossbreed;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, elder generation's beta pruning, then the regrowth that sends is reached the cotton stake of 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, blooms the knot bell phase, utilize insect pollination or artificial pollination, and the educated strain that stay last year made marks again, the seed behind the knot bell in the sterile strain of results is for the use of preparation cotton crossbreed;
D, repetition B, C step 3 times.Also be that this routine method can be sowed in 1 year, gathered in the crops the seed that produces on the vegetative plant in continuous 5 years.
Embodiment two
The present invention utilizes perennial root to carry out the asexual reproduction method of cotton nucleic sterility restoring line or common cotton strain, the steps include:
A, be winter minimum temperature in the production of hybrid seeds district that cotton plants can be survived the winter greater than 10 ℃ area, plant cotton nucleic sterility restoring line or common cotton strain are bloomed the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, when the regrowth that sends in the cotton stake that beta pruning is stayed reaches the 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant is for using;
D, repetition B, C step 3 times.D, repetition B, C step 3 times.Also be that this routine method can be sowed in 1 year, gathered in the crops the seed that produces on the vegetative plant in continuous 5 years.
The present invention can also only repeat two step 1～2 of B-C time when implementing, promptly once sow continuous 3 years or 4 years results seeds.But generally surpass 5 years is after the 6th year, and growing way of perennial root cotton and hybrid vigour promptly produce degeneration and should not reuse.
The female parent of the present invention's breeding can be existing various genic male sterility dual purpose cotton strain, male parent can be various existing nucleic sterility restoring lines, can be various good quality and high output cotton strains, if these cotton strains in the winter time minimum temperature greater than 10 ℃ the area survive the winter naturally.
The concrete field management of the present invention when implementing should be adopted the existing cultivation management measure of corresponding strain according to different cotton strains.
Claims (2)
1. utilize perennial root to carry out the asexual reproduction method of cotton kernel sterility dual serial, the steps include:
A, minimum temperature is greater than 10 ℃ area in the winter time, the plant cotton kernel sterility dual serial, and the identification of flower bud phase can be educated strain and sterile strain, pulls out in the cotton field 4/5～3/5 educated strain, and mark is done in remaining 1/5～2/5 educated strain; Bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell in the sterile strain of results uses for the preparation cotton crossbreed;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, when the regrowth that sends in the cotton stake after the beta pruning reaches the 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, bloom the knot bell phase, utilize insect pollination or artificial pollination, and the educated strain that stay last year is made marks again, seed behind the knot bell in the sterile strain of results uses for the preparation cotton crossbreed;
D, repetition B, C step 1～3 time.
2. utilize perennial root to carry out the asexual reproduction method of cotton nucleic sterility restoring line or common cotton strain, the steps include:
A, minimum temperature is greater than 10 ℃ area in the winter time, and plant cotton nucleic sterility restoring line or common cotton strain are bloomed the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant;
B, gathered in the crops seed after, all cotton plants are stayed in the cotton field and are survived the winter naturally, carry out perennial root;
C, next year are pruned with fruittree shear the cotton plant of staying in the cotton field, when the regrowth that sends in the cotton stake that beta pruning is stayed reaches the 3-5 root, repair, every is stayed 1 regrowth the most healthy and the strongest, bloom the knot bell phase, utilize insect pollination or artificial pollination, the seed behind the knot bell on the results cotton plant is for using;
D, repetition B, C step 1～3 time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100496562A CN101107916B (en) | 2007-08-02 | 2007-08-02 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100496562A CN101107916B (en) | 2007-08-02 | 2007-08-02 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
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| Publication Number | Publication Date |
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| CN101107916A CN101107916A (en) | 2008-01-23 |
| CN101107916B true CN101107916B (en) | 2010-04-07 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598084A (en) * | 2013-10-23 | 2014-02-26 | 中国农业科学院棉花研究所 | Seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization |
| CN104160952B (en) * | 2014-08-27 | 2016-06-08 | 四川省农业科学院经济作物育种栽培研究所 | The method utilizing the continuous breeding seed of perennial root F1 generation |
| CN106034643B (en) * | 2016-06-02 | 2020-02-14 | 石河子农业科学研究院 | Method for storing and utilizing northern wild cotton germplasm resources |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276964A (en) * | 2000-08-09 | 2000-12-20 | 四川省农业科学院经济作物育种栽培研究所 | Production process of Za-13 as high-quality disease and pest resisting hybrid cotton |
| CN1378777A (en) * | 2002-04-26 | 2002-11-13 | 四川省农业科学院经济作物育种栽培研究所 | Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization |
| CN101124886A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Using with cotton sterile male line and recovery line to processing ratoon regenerating hybrid seed production method |
| CN101124885A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
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- 2007-08-02 CN CN2007100496562A patent/CN101107916B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276964A (en) * | 2000-08-09 | 2000-12-20 | 四川省农业科学院经济作物育种栽培研究所 | Production process of Za-13 as high-quality disease and pest resisting hybrid cotton |
| CN1378777A (en) * | 2002-04-26 | 2002-11-13 | 四川省农业科学院经济作物育种栽培研究所 | Bee pollination technique for breeding and reproducing nuclear male sterility line of cotton by hybridization |
| CN101124886A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Using with cotton sterile male line and recovery line to processing ratoon regenerating hybrid seed production method |
| CN101124885A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
Non-Patent Citations (1)
| Title |
|---|
| 张小红等.宿根条件下的棉花核不育杂交制种技术研究.中国棉花学会2008年年会论文汇编.2008,(2008),第189-191页. * |
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