CN104160952B - The method utilizing the continuous breeding seed of perennial root F1 generation - Google Patents
The method utilizing the continuous breeding seed of perennial root F1 generation Download PDFInfo
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- CN104160952B CN104160952B CN201410427612.9A CN201410427612A CN104160952B CN 104160952 B CN104160952 B CN 104160952B CN 201410427612 A CN201410427612 A CN 201410427612A CN 104160952 B CN104160952 B CN 104160952B
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Abstract
Present invention one specifically utilizes perennial root F1For the method for continuous breeding seed, comprise the steps: A, Selection parent, preparing hybrid F1For seed, select F1Combination, and to F1Test and resistance, insect resistance identification is compared, it is provided that participate in various regional testing and by authorizing, obtain the legitimacy at region utilization and extention definitely for cenospecies; B, plant cotton F1For cenospecies, carry out perennial root; C, the F gathered in the crops then2Experiment and demonstration is carried out in cotton region for cenospecies seed; D, the 2nd��5 year results F2For cenospecies seed. The inventive method, it is possible to save F1Consumption for cenospecies; Sowing in 1 year is gathered in the crops for years, it is ensured that breeding F2Quality for seed has concordance; Utilizing this technology 1 year sowing to gather in the crops, purity and the quality of seed are more guaranteed, decrease the sport technique segments such as sowings in 2��5 years for years, reduce planting cost, the cotton benefit of planting of peasant household improves further.
Description
Technical field
The present invention relates to crop breeding technical field, one utilizes perennial root F specifically1Method for continuous breeding seed.
Background technology
Cotton Gossypii is often cross-pollinated crop, and generally outcrossing rate is 3��10%, may be up to 50% when insect pollinator is more; It addition, its parents of cross combination of selection-breeding at present self exist certain loss of heterozygosity, even if the flower selfing of completely isolated bolt, its filial generation and parental generation also can morph because of gene recombinaton.
People are in the process of breeding parent, always excellent eliminate bad method according to selecting, if logical completely isolated breeding in a few years, its parents be likely to than combination selection time be intended to isozygoty or excellent, but due to the change of parents, the specific combining ability of its combined hybrid advantage also changes therewith; In a few years non-fully isolate breeding if logical, then the existing outcrossing of parents has again and self recombinate, and the change that its parent and offspring combine is bigger.
Utilize Cotton Gossypii perennial root technology, perennial root F1For cenospecies, it is possible to effectively overcome the impact on hybrid seed quality due to hybrid parents variation.
Summary of the invention
It is an object of the present invention to provide one and utilize perennial root F1For the method for continuous breeding seed, the method need not plant F every year1For cenospecies, sow the labour force with thinning after eliminating Second Year and put into, reduce the input of hybrid seeding, it is ensured that seed quality. The parent of the method from the second year, bloom ahead of time, and yield is higher by 20��40% than First Year, improves production of hybrid seeds benefit by parent. F1Generation sowing once, utilizes for many years, and production of hybrid seeds place is relatively stable, and production of hybrid seeds scale is easily controlled, and can be easy to carry out commercial scale application.
For achieving the above object, the technical solution used in the present invention is: one utilizes perennial root F1For the method for continuous breeding seed, comprise the steps:
The parent that A, selection field trait expression are consistent, utilizes artificial emasculation technology preparing hybrid F1For seed, select high advantage F by comparative test1Combination, and to F1Test and Resistance Identification is compared, it is provided that participate in various regional testing and by authorizing, obtain the legitimacy at certain area utilization and extention for cenospecies;
B, the area can survived the winter at cotton plants, plant cotton F1For cenospecies, being managed according to common land for growing field crops Cotton Gossypii, after having gathered in the crops, all cotton plants stay overwintering in cotton field, carry out perennial root;
C, the F gathered in the crops then2Experiment and demonstration is carried out in cotton region for cenospecies seed;
D, perennial root cotton within the 2nd��5 year, is utilized to continue results gined cotton and F2For cenospecies seed;
In step A, described parent is non-sterile system parent; Described artificial emasculation technical step is: by the female parent of non-sterile system and paternal hybrid, obtain the F of artificial emasculation in maternal colony1Hybrid cotton seed.
Further, in step B, described Cotton Gossypii area of can surviving the winter refers to height above sea level less than 1200 meters, and light, heat are abundant, winter warm summer heat, climate simulation is little, round the clock temperature great disparity, and dry and wet is clearly demarcated for season, dry season many strong wind, rainy season many showers, heavy rain and night rain, the area that evaporation capacity is big.
Further, in step B, described perennial root is to have gathered in the crops the F of seed1Retaining its root and the stem of 1��5 leaf position, ground for plant, survive the winter, keep its root and the vigor of stayed aerial parts under natural environmental condition, next year is gone out sprouting by upper grown, proceeds every management, gathers in the crops hybrid seed and gined cotton autumn.
The method have the benefit that:
(1) the inventive method is utilized, it is possible to save F1Consumption for cenospecies;
(2) the inventive method sowing in a year is gathered in the crops for years, it is ensured that breeding F2Quality for seed has concordance.
(3) utilizing this technology 1 year sowing to gather in the crops, purity and the quality of seed are more guaranteed, decrease the sport technique segments such as sowings in 2��5 years for years, reduce planting cost, the cotton benefit of planting of peasant household improves further.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment 1
One, the natural conditions of Cotton Gossypii parent's perennial root are met
1, temperature:
(1), average annual temperature 20��24 DEG C, the effective accumulated temperature of >=10 DEG C is more than 7000 DEG C.
(2), JIUYUE to monthly mean temperature more than 12 DEG C in February next year, 3��April monthly mean temperature 22��23 DEG C, 5��August monthly mean temperature more than 25 DEG C.
(3), the natural law of frost occurs morning in January below 10 days, other in month free of frost.
(4), height above sea level less than 1200 meters.
2, illumination: year sunshine time be 1700��2100h, percentage of sunshine 50��60%.
3, humidity: annual rainfall 800��1200, is mainly distributed on late May to late September. Needing arid winter, humidity is little.
Total feature is: light, heat are abundant, winter warm summer heat, and climate simulation is little, round the clock temperature great disparity, and dry and wet is clearly demarcated for season, dry season many strong wind, rainy season many showers, heavy rain and night rain, evaporation capacity is big.
Two, place and material are implemented:
The area of Panzhihua City Renhe District, Sichuan Province height above sea level less than 1200 meters, same parent material must be independently selected in a completely isolated area. Enforcement parent material is ZR5 and G5, utilizes G5 for female parent, and ZR5 is male parent, prepares artificial emasculation cross combination H23.
Three, pest-resistant F1Field practice technology for cross combination H23 (non-perennial root, i.e. S0 generation)
1, sowing and seedling management:
(1) nursery and seedbed management: selecting hidding and sunny side place to make seedbed, railway carriage or compartment opened by 4��4.5 chis, and mu is with 5000��6000 lattice, every mu of seedbed carbamide 1kg, phosphate fertilizer 1.5kg, oil cake 2kg, be divided into little lattice with cutter after mixing fertilizer and soil thoroughly latting drown leveling with plain boiled water, ensure every lattice 2��2.5 cun, irrigated land should be sowed mid-March to early April, and before sowing, seed hot water steeps more than 2 hours, mixes the anti-damping-off of carbendazim, every lattice 2, mu is with 2.5 jin of seeds. Slurry railway carriage or compartment is watered wants many, too not dry during blinding, the sagitta 40��50cm of covering with ground sheeting; If temperature reaches more than 30 DEG C during sowing, seedbed not practicably film cover, cover with millet straw, so as not to temperature be too high and burn seedlings. Seedling notes taking off film (taking off grass) seedling exercising, first two, afterwards one side of something after all sprouting, more entirely takes off. Generally not watering in seedbed, seedling stage mainly prevents and treats damping-off, aphid, caterpillar, cotten aphid etc.
(2) transfer: transplant for after nursery 20��30 days, adopt the plantation of size row, big row 3 chi, little row 2 chi, spacing in the rows 1.2 chi; Also row plantation, line-spacing 3 chi, spacing in the rows 0.9��1.0 chi can be waited. Every mu of 2000 nests, transplant by 1:2:1 mode, i.e. double; two strains and the alternate transplanting of individual plant, and density is 3000 plants/acre. During transplanting, mu is with dry mud miscellaneous fertilizers 450��500kg, phosphate fertilizer 20kg, carbamide 7.5��10kg, oil cake 10kg, potash fertilizer 5��10kg, loses and waters in nest, puts Seedling blinding.
2, flower bud period management
(1), training: arrange leaf branch and axillalry bud in time, can control to nourish and grow, regulate the nutrient allocation in cotton plant body, reduce the effective consumption of nutrient, be conducive to the growth of flower bud, Hua Heling.
(2), clamp railway carriage or compartment is fertile: pulling out and use before and after fertile plant, mu carbamide 5��10kg, phosphate fertilizer 30kg, potash fertilizer 7.5kg, oil cake 20kg, slag fertilizer 400kg, fertilization mode nest is executed.
(3), chemical regulation: mu casing coupling pacifies 1��1.5g, is watered 15��25kg, and effect is strong flower bud prematurity.
(4), cultivation and banking: fertilising ridging after first weeding before the fertilizer of clamp railway carriage or compartment, to promote to prick under root system, Spindling control, during cotton bud stage, this area has located rainy season, and quickly, weeding is one of important process to weed growth.
(5), prevention and elimination of disease and pests: generally requiring preventing and treating in 7��10 days once, primary pest is aphid, bollworm, red spider etc., and general organophosphorus pesticide can be prevented and treated.
3, flowering and boll-setting period management
(1), heavy dressing flower bell is fertile: having during 2��3 hard Fructus Persicae and use, mu carbamide 50��80kg, potash fertilizer 5kg nest is executed.
(2), chemical regulation: entering florescence mu casing coupling peace 1.5��2g and add water 30kg, beat once in 10��15 days, increase to 3��4g after pinching, its effect is to mould ideotype, postpones sealing and goes.
(3), bud is removed in training: 13��16, irrigated land, 8��12, mountain region point of pinching, destroy escenter and bud from bottom to top gradually, in order to avoid increase the moon covers and causes rotten bell, remove invalid alabastrum when pollination terminates according to practical situation, cut sky branch and stem bottom Lao Ye, increase ventilation and penetrating light, reduce rotten bell, reduce Nutrient loss.
(4), benefit executes caping fertilizer: takes the circumstances into consideration to use according to growing way, can execute 10��15kg carbamide by nest, it is possible to foliage top dressing.
4, management in the term of opening bolls:
(1), foliage application: the cotton field for de-fertilizer or fertility difference can adopt foliage application, with the urea liquid of 1%, each every mu sprays 45��60kg solution, every spray in 8��10 days once, even spray 2��3 times, it is also possible to the potassium dihydrogen phosphate of 2% carries out foliage-spray.
(2), later stage training: cut the superfluous bud of sky branch.
5, perennial root cotton plant is retained:
After harvesting unginned cotton (about about 30 days October), the whole strain of cotton plant is stayed in soil, and cotton plant in the ranks can not water and plant short season vegetable etc.
Four, pest-resistant F1Field practice technology for cross combination H23 (perennial root, i.e. S1, S2, S3, S4, S5 generation)
1, beta pruning: mid-February to early March, to the cotton plant (F stayed in cotton field1For plant) prune with fruittree shear. Pruning method is: from ground, upwards 10��15cm cuts off stem, and 2��3 leaf segments are stayed in every strain.
2, pruning and stay Seedling: irrigated land mid or late March, in first 10��15 days of rainy season of mountain region, the regrowth that the cotton stake that beta pruning is stayed sends is repaired. Its method is: all erased by the seedlings in cotton stake, and every is stayed that 1 the most healthy and the strongest and the regrowth that just sent.
3, seedling management: irrigated land manages, first water to apply fertilizer afterwards in late March and carry railway carriage or compartment again, (beating nest between two strain cotton stakes) dry mud miscellaneous fertilizers 450��500kg executed by every mu of nest, oil cake 10kg, carbamide 7.5��10kg, phosphate fertilizer 20kg, potash fertilizer 7.5��10kg, rain is increased soil fertility and is only carried out weeding before rainfall and carry railway carriage or compartment. Can the management in this period have important function to obtain high yield, it focuses on cultivating healthy and strong Seedling frame, flower for nourishing and growing on more weak regrowth must remove, attention control insect pest simultaneously and removing weeds, the rain that can not water increase soil fertility block until rainy season first 5 days start basal dressing, fertilizing method and quantity are the same with irrigated land.
4, flower bud period management: field management is as fertile in executed big nest, it is identical that weeding, training, change control, the prevention and control of plant diseases, pest control etc. manage on behalf of another reason with S0, decreases identification and pulls out the link of part fertile plant. Additionally in those more weak fields of nourishing and growing, 1��2 Fruit branch or front 2��5 alabastrums can be removed, reach to a certain degree ensureing that it is nourished and grown.
5, flowering and boll-setting period management: perennial root is cotton and non-perennial root is cotton essentially identical in the management of flowering and boll-setting period, removes bud etc. including heavy dressing flower bell fertilizer, chemical regulation, training.
6, management in the term of opening bolls: bloom knot bell phase (mid-May to mid-August) cotton due to perennial root is long, its term of opening bolls (mid-July to late October) also extends accordingly, and 7��August this area just rainy season, therefore diligent flower must be received, rob fine flower of receiving and shine flower, guarantee that kind of unginned cotton will not be subject to wet, it is ensured that the germination percentage of seed. Other are with the management in S0 generation.
7, retain perennial root cotton plant: the same with S0 generation, still cotton plant is retained in order to continuing the perennial root production of hybrid seeds next year.
8, within 3��5 years, continue to repeat above-mentioned work, complete the production of surface and seed.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (3)
1. utilize perennial root F1Method for continuous breeding seed, it is characterised in that comprise the steps:
The parent that A, selection field trait expression are consistent, utilizes artificial emasculation technology preparing hybrid F1For seed, select high advantage F by comparative test1Combination, and to F1Test and Resistance Identification is compared, it is provided that participate in various regional testing and by authorizing, obtain the legitimacy at certain area utilization and extention for cenospecies;
B, the area can survived the winter at cotton plants, plant cotton F1For cenospecies, being managed according to common land for growing field crops Cotton Gossypii, after having gathered in the crops, all cotton plants stay overwintering in cotton field, carry out perennial root;
C, the F gathered in the crops then2Experiment and demonstration is carried out in cotton region for cenospecies seed;
D, perennial root cotton within the 2nd��5 year, is utilized to continue results gined cotton and F2For cenospecies seed;
In step A, described parent is non-sterile system parent; Described artificial emasculation technical step is: by the female parent of non-sterile system and paternal hybrid, obtain the F of artificial emasculation in maternal colony1Hybrid cotton seed.
2. utilize perennial root F according to claim 11Method for continuous breeding seed, it is characterized in that: in step B, described Cotton Gossypii area of can surviving the winter refers to height above sea level less than 1200 meters, light, heat are abundant, winter warm summer heat, and climate simulation is little, round the clock temperature great disparity, dry and wet is clearly demarcated for season, dry season many strong wind, rainy season many showers, heavy rain and night rain, the area that evaporation capacity is big.
3. utilize perennial root F according to claim 11Method for continuous breeding seed, it is characterised in that: in step B, described perennial root is to have gathered in the crops the F of seed1Retaining its root and the stem of 1��5 leaf position, ground for plant, survive the winter, keep its root and the vigor of stayed aerial parts under natural environmental condition, next year is gone out sprouting by upper grown, proceeds every management, gathers in the crops hybrid seed and gined cotton autumn.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101107916A (en) * | 2007-08-02 | 2008-01-23 | 四川诺亚生物科技有限公司 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
| CN101124885A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
| CN101485260A (en) * | 2009-02-25 | 2009-07-22 | 广西大学 | Perennial cultivation method for annual cotton |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6998519B2 (en) * | 2003-07-22 | 2006-02-14 | D&Pl Technology Holding Company, Llc | Cotton cultivar 01Z34 |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107916A (en) * | 2007-08-02 | 2008-01-23 | 四川诺亚生物科技有限公司 | Asexual propagation method of gossypii nuclear sterile dual-purpose line, restoring line and ordinary line by using biennial root |
| CN101124885A (en) * | 2007-08-24 | 2008-02-20 | 四川省农业科学院经济作物育种栽培研究所 | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon |
| CN101485260A (en) * | 2009-02-25 | 2009-07-22 | 广西大学 | Perennial cultivation method for annual cotton |
Non-Patent Citations (4)
| Title |
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| Expression of genes associated with carbohydrate metabolism in cotton stems and roots;EarlWTaliercio et al.;《BMC Plant Biology》;20090122;第9卷(第11期);第1-10页 * |
| 宿根条件下的棉花核不育杂交制种技术研究;张小红等;《中国棉花学会2008年年会论文汇编》;20081231;第189-191页，尤其是对比文件1第1.1-3.4节 * |
| 棉花宿根制种技术研究初探;张相琼等;《中国棉花学会2009年年会论文汇编》;20101231;第209-211页 * |
| 棉花抗虫核不育系抗A4的培育;张小红等;《中国棉花学会2011年年会论文汇编》;20111231;第172-174页 * |
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