CN105706901B - With leaf margin totally cleave marking character rape sterile line and selection and application - Google Patents
With leaf margin totally cleave marking character rape sterile line and selection and application Download PDFInfo
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- CN105706901B CN105706901B CN201610065700.8A CN201610065700A CN105706901B CN 105706901 B CN105706901 B CN 105706901B CN 201610065700 A CN201610065700 A CN 201610065700A CN 105706901 B CN105706901 B CN 105706901B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The rape sterile line breeding method of marking character of the present invention of being totally cleaved with leaf margin, by using with leaf margin totally cleave marking character the stabilization that obtains of plant and rape distant hybridization seed selection with leaf margin totally cleave marking character rape self-mating system as male parent, to produce excellent rape sterile line as female parent, selection cross obtains to totally cleave the rape sterile line of marking character with leaf margin, and the marking character of totally cleaving of the leaf margin in the rape sterile line of the marking character of being totally cleaved with leaf margin of acquisition has strong specificity.Leaf margin totally cleaves character of the marking character for a pair of dominant major gene resistances control, character is stable, not by environmental influence, leaf margin totally cleave carve character it is deeper, character is obvious, easily identify, can early find, it can typically distinguish when seedling true leaf occurs, in sterile line reproductive process, be totally cleaved the plant at quarter beneficial to identifying and pulling out all non-leaf margins, prevent from mixing other seeds, ensure male-sterile seed purity.
Description
Technical field
The invention belongs to crop breeding technical field, and in particular to a kind of rape for marking character of being totally cleaved with leaf margin
Sterile line and selection and application.
Background technology
At present, 80% Hybrid used above in China's Rape-seed production.Hybrid is miscellaneous including three line hybrid seed, two
Hand over kind.
The production of three line hybrid seed includes restorer breeding, maintainer breeding, sterile line propagation and hybrid seeding.Specific mistake
Cheng Shi：Restorer isolation breeding seed, maintainer isolation breeding seed；Sterile line hybridizes breeding by sterile line and maintainer；Greatly
Field production by sterile line and recovers line hybrid breeding acquisition with hybrid.
The production of two-series hybrid includes：The breeding of restorer, the breeding of sterile line and hybrid seed production.Rape cell
Shown in matter malesterile three-line seed flow as Figure of description Fig. 6.Rapeseed cytoplasmic male sterile two is that production of hybrid seeds flow is shown in
Shown in bright book accompanying drawing Fig. 7.
Because the restorer and sterile line phenotypic character of cabbage type rape are not easily distinguishable, current Brassica napus hybrid kind
Three during the production of hybrid seeds be present：One, in sterile line reproductive process, it is difficult to remove hybrid strain, easily directly contributes the production of hybrid seeds with not
It is that parent is impure to educate, and so as to cause the first generation of hybrid impure, commodity seed production and business risk are larger；Two, production of hybrid seeds production process
In, pollination will cut off restorer after spending eventually, because restorer and sterile line phenotypic character are not easily distinguishable, easily cause and cut off recovery
The sterile line after pollination is cut off when being, so as to cause hybrid seed yield to reduce, increases breeding cost；Three, its F1 generation cenospecies
If pseudostationary in (field production production kind), it is impossible to find, can be caused to production sternly in time in Production of Large Fields Seedling Stage
Lose again.Therefore, differentiate that the purity of colony and true and false hybrid are always what rapeseed breeding researcher was pursued with mark property
One of target.
At present, scientific research personnel is attempted with lobus cardiacus purple mark property, yellow seedling mark property, floral leaf mark property, shirt rim leaf
Mark property, lug mark property, purple stem mark property, wax powder-free mark property, white flower mark property, orange colored mark property
Cultivate the breeding material with mark property Deng mark property, but these characters are had a great influence by environment, especially by temperature,
The external environments such as illumination have a great influence so that it can not reach the effect of mark property, in actual applications by certain limit
System.Such as by the use of lobus cardiacus purple as mark property, in production of hybrid seeds production process, being declined in seedling stage by autumn and winter temperature is influenceed,
General rape can all change colour, and be not easy to differentiate, or when flower eventually, the complete leaf for having leaf color difference has all changed colour, it is difficult to differentiate, or
It has been come off that, also do not reach the effect of mark without practical value.For another example Chinese patent literature 01108531.2 discloses one
The method that kind identifies true and false kind using wax powder-free genetic marker, mark maintainer, sterile line or restorer are marked with wax powder-free
Turn seed selection, combined using the restorer or sterile line breeding advantage cross-bred rape for carrying wax powder-free genetic marker, in advantageous combination
When Parent separated according to genetic marker, non-parent's strain is rejected, after harvest production of hybrid seeds area seed, according to there is wax powder-free genetic marker.
Although such scheme can distinguish sterile line and restorer, hybrid strain is rejected, identifies true and false hybrid, the wax powder-free in the program
The actual simultaneously unobvious of genetic marker, are difficult to, operation difficulty is high, and workload is big, holds in identification process in Production of Large Fields
Easily retain false kind.Therefore, totally cleaved marking character mark rape sterile line the invention provides a kind of leaf margin so that it is from seedling
The appearance beginning of phase true leaf is just extremely obvious with ordinary cole difference, also has notable difference, Ke Yida even if the incomplete leaf in later stage
Effect is marked to preferable character.
The content of the invention
Therefore, the technical problem to be solved in the present invention is to overcome the mark property unobvious of rape in the prior art, no
It is stable, it is easily affected by environment, it is not easy to differentiate the purity of colony and true and false hybrid, a kind of character is obvious, character is steady so as to provide
It is fixed, be not easy it is affected by environment with leaf margin totally cleave marking character rape sterile line and its selection and application, reflecting
Significant effect in terms of the purity of other colony and true and false hybrid.
Therefore, totally cleaved the rape sterile line breeding method of marking character with leaf margin the invention provides a kind of, with band
There is the totally cleave stabilization that obtains of plant and rape distant hybridization seed selection of marking character of leaf margin to totally cleave blaze memory with leaf margin
The rape self-mating system of shape is male parent, and to produce excellent rape sterile line as female parent, selection cross obtains to totally cleave quarter with leaf margin
The rape sterile line of mark property.
The rape sterile line breeding method of described marking character of being totally cleaved with leaf margin, comprises the following steps：
(1) selection is using the sterile plant frequency of genotypes AA CC of the excellent rape sterile line of the production as female parent, with the stabilization
Have leaf margin totally cleave marking character rape self-mating system frequency of genotypes AA BBCC for paternal hybrid obtain F1For genotype
AABCC, with F1It is selfed for plant bagging and obtains F2For segregating population, from the F2For obtaining totally cleaving with leaf margin in segregating population
The sterile individual plant frequency of genotypes AA BBCC of marking character；
(2) using obtain in step (1) with leaf margin totally cleave marking character sterile individual plant frequency of genotypes AA BBCC to be female
This, using the stabilization there is the totally cleave rape self-mating system frequency of genotypes AA BBCC of marking character of leaf margin to carry out backcrossing as male parent and obtain
Obtain BC1F backcross populations；
(3) from the BC1Selection is totally cleaved the sterile individual plant genotype of marking character with leaf margin in F backcross populations
AABBCC for female parent, using the stabilization have leaf margin totally cleave marking character rape self-mating system frequency of genotypes AA BBCC as male parent
Continuous backcross 2-3 generations, from the backcross population obtained selection totally cleaved the sterile individual plant genotype of marking character with leaf margin
AABBCC, it is the rape sterile line frequency of genotypes AA BBCC of the marking character of being totally cleaved with leaf margin.
Preferably, the cabbage type rape is cabbage type rape new lines (RS013-008).
The rape sterile line breeding method of described marking character of being totally cleaved with leaf margin, it is characterised in that also including will
The rape sterile line individual plant frequency of genotypes AA BBCC continuous bag sleevings for the described marking character of being totally cleaved with leaf margin that step (3) obtains
Selfing 3 is commissioned to train the step of educating.
The rape sterile line breeding method of described marking character of being totally cleaved with leaf margin, in addition to step (3) is obtained
It is described with leaf margin totally cleave the rape sterile line individual plant frequency of genotypes AA BBCC of marking character and production to go up key rape extensive
It is frequency of genotypes AA CC test crosses again, filters out fertile rate up to 100%, and the maternal strain that hybrid vigour is strong and Comprehensive Traits are good is
The rape sterile line frequency of genotypes AA BBCC of described marking character of being totally cleaved with leaf margin.
The rape sterile line breeding method of described marking character of being totally cleaved with leaf margin, with Genotypes of Brassica napus
AACC is female parent, and belong to Ye Sheng indian rorippa herb genotype BB using Shi word Hua Ke indian rorippa herbs carries out the distant hybridization seed selection acquisition stabilization as male parent
Totally cleaved the rape of marking character with leaf margin.
The rape sterile line breeding method of described marking character of being totally cleaved with leaf margin, the stabilization it is complete with leaf margin
The rape seed selection acquisition by the following method of marking character is split, is comprised the following steps：
A) using Genotypes of Brassica napus AACC as female parent, Ye plant Zi Yuan indian rorippa herb genotype is belonged to Shi word Hua Ke indian rorippa herbs
BB is that male parent carries out distant hybridization acquisition F1For genotype ABC, with F1It is selfed for plant bagging and obtains F2For segregating population, from F2
For the individual plant frequency of genotypes AA BBCC that marking character of being totally cleaved with leaf margin is obtained in segregating population；
B) using F2 segregating populations obtain with leaf margin totally cleave marking character individual plant frequency of genotypes AA BBCC to be maternal, with
Genotypes of Brassica napus AACC is that male parent backcrossing obtains BC1F backcross populations；
C) from the BC1In F backcross populations selection with leaf margin totally cleave marking character individual plant frequency of genotypes AA BBCC for
Female parent, using Genotypes of Brassica napus AACC as the generation of male parent continuous backcross 2, obtain BC3F1 backcross populations；
D) from the BC3In F1 backcross populations selection with leaf margin totally cleave marking character individual plant frequency of genotypes AA BBCC connect
Continuous bagging was selfed for 3 generations, cultivated the stable Rape Germplasm Resources frequency of genotypes AA BBCC with marking character of totally cleaving, and was steady
The rape self-mating system of fixed marking character of being totally cleaved with leaf margin.
The invention provides it is a kind of by above-mentioned method cultivate to obtain with leaf margin totally cleave marking character rape not
It is frequency of genotypes AA BBCC to educate.
The invention provides it is a kind of it is described with leaf margin totally cleave marking character rape sterile line application, utilize institute
Rape sterile line frequency of genotypes AA BBCC and the common blade profile cabbage type rape restorer for the marking character of being totally cleaved with leaf margin stated
Frequency of genotypes AA CC test crosses, obtain the cross rape frequency of genotypes AA BCC for marking character of being totally cleaved with leaf margin.
The invention provides it is a kind of it is described with leaf margin totally cleave marking character rape sterile line application, with described
With leaf margin totally cleave marking character rape sterile line frequency of genotypes AA BBCC for female parent, totally cleaved quarter with leaf margin with described
The rape self-mating system frequency of genotypes AA BBCC of mark property is that male parent is hybridized, and obtains the oil for marking character of being totally cleaved with leaf margin
Dish sterile line homotype maintainer frequency of genotypes AA BBCC.
The invention provides it is a kind of it is described with leaf margin totally cleave marking character rape sterile line application, with described
With leaf margin totally cleave marking character rape sterile line frequency of genotypes AA BBCC for female parent, totally cleaved quarter with leaf margin with described
The rape self-mating system frequency of genotypes AA BBCC of mark property is to obtain in the generation of male parent continuous backcross 2 homozygous to totally cleave blaze memory with leaf margin
The rape sterile line frequency of genotypes AA BBCC of shape.
It is sterile line model 99A01 that excellent rape sterile line is produced in the present invention；Cabbage type rape new lines (RS013-
008)；Shi word Hua Ke indian rorippa herbs category Ye Sheng indian rorippa herb models A985054；Common blade profile cabbage type rape restorer model RS013-008；
Key rape restorer model C 01 in production；It is above-mentioned to be provided by Jiangxi Academy of Agricultural Sciences Crop Institute.
Technical solution of the present invention compared with prior art, has the following advantages that：
(1) it is of the present invention to be totally cleaved the rape sterile line breeding method of marking character with leaf margin, by with
The totally cleave stabilization that obtains of plant and rape distant hybridization seed selection of marking character of leaf margin is totally cleaved marking character with leaf margin
Rape self-mating system be male parent, to produce excellent rape sterile line as female parent, selection cross obtains to totally cleave blaze with leaf margin
The rape sterile line of memory shape, acquisition it is described with leaf margin totally cleave marking character rape sterile line in leaf margin totally cleave quarter
Mark property has strong specificity, is the character of a pair of dominant major gene resistances control, and character is stable, not by environmental influence,
Leaf margin totally cleave carve character it is deeper, character is obvious, easily identification, can early find, can typically be distinguished when seedling true leaf occurs,
In sterile line reproductive process, totally cleaved the plant at quarter beneficial to identifying and pulling out all non-leaf margins, prevent from mixing other seeds, ensure not
It is seed purity to educate；
During the first generation of hybrid production of hybrid seeds of Production of Large Fields kind, readily identified restorer and not breeding system, in sterile line
After spending eventually, it is easier thoroughly to cut off restorer, does not allow fallibility to cut sterile line again, ensures seed purity and the production of the first generation of hybrid
Amount；
On Production of Large Fields, true and false hybrid can be differentiated in time, reduce thus caused Seed enterprises with Rape-seed production person's
Seed dispute；
(2) the rape sterile line breeding method of marking character of the present invention of being totally cleaved with leaf margin, by with wild cabbage
Type rape line (RS013-008) is female parent, and belong to Ye Sheng indian rorippa herbs using Shi word Hua Ke indian rorippa herbs carries out distant hybridization seed selection as male parent
Obtain the stabilization with leaf margin totally cleave produced described in the rape of marking character excellent rape sterile line sterile plant it is miscellaneous
The rape sterile line of the marking character of being totally cleaved with leaf margin obtained is handed over, is advantageous to improve hybrid seed yield and unit yield,
The rape sterile line of the marking character of being totally cleaved with leaf margin, some of favorable genes derive from wild plant resource, with
Relatively far away from, both are beneficial to new group of the strong advantage cross-bred rape of seed selection at hybridization to common blade profile cabbage type rape restorer affiliation
Close, so as to improve hybrid seeds yield and the upper unit yield of production, meanwhile, the Hybrids F1 leaf margin of cultivation, which totally cleaves, carves character, due to
Leaf area is small with respect to cabbage type rape, beneficial to planting density is improved, makes full use of unit area luminous energy, effectively improves light and effect
Rate, further improve rape specific yield；
(3) it is of the present invention with leaf margin totally cleave marking character rape sterile line application, utilize described band
There is leaf margin to totally cleave rape sterile line and the common blade profile cabbage type rape restorer test cross of marking character, obtain complete with leaf margin
The cross rape of marking character is split, the cenospecies is beneficial to resistance of the enhancing to sclerotinia sclerotiorum, due to utilizing cultivation
The strong advantage hybrid Combination nova F1 generation plant that sterile line is cultivated shows as leaf margin entirely totally cleaves and carves character, in production on whole florescence blade face
The fallen flowers of carrying are less, correspondingly reduce the sclerotiniose incidence of disease.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the rape sterile line breeding flow of the marking character of being totally cleaved with leaf margin described in the embodiment of the present invention 1
Figure；
Fig. 2 is the rape seed selection flow chart of marking character of being totally cleaved with leaf margin stable described in the embodiment of the present invention 1；
Fig. 3 is rape sterile line and the common blade profile of the marking character of being totally cleaved with leaf margin described in the embodiment of the present invention 1
Cabbage type rape restorer test cross flow chart；
Fig. 4 is the cabbage type rape sterile line breeding flow chart described in the embodiment of the present invention 4；
Fig. 5 is the rape sterile line infertility of the described marking character of being totally cleaved with leaf margin of the seed selection of the embodiment of the present invention 3
The comparison figure of the cabbage type rape sterile line sterile plant of strain and the seed selection of embodiment 4；
Fig. 6 is rape cytoplasm male sterility three line production of hybrid seeds flow chart in background of invention；
Fig. 7 is that rapeseed cytoplasmic male sterile two is production of hybrid seeds flow chart in background of invention.
Embodiment
Embodiment 1
The rape sterile line breeding method of the described marking character of being totally cleaved with leaf margin of this implementation, it specifically includes as follows
Step：
The stabilization with leaf margin totally cleave marking character rape selection flow as shown in Fig. 2 its is specific
Comprise the following steps：
A) using cabbage type rape (RS013-008) AACC as female parent, Ye plant Zi Yuan indian rorippa herbs are belonged to Shi word Hua Ke indian rorippa herbs
BB is that male parent carries out distant hybridization acquisition F1For ABC, with F1It is selfed for plant bagging and obtains F2For segregating population, from F2Generation separation
The individual plant AABBCC for marking character of being totally cleaved with leaf margin is obtained in colony；
B) using F2 segregating populations obtain with leaf margin totally cleave marking character individual plant AABBCC to be maternal, with Wild cabbage type
Rape (RS013-008) AACC is that male parent backcrossing obtains BC1F backcross populations；
C) from the BC1In F backcross populations selection with leaf margin totally cleave marking character individual plant AABBCC be it is maternal, with
Cabbage type rape new lines (RS013-008) AACC is the generation of male parent continuous backcross 2, obtains BC3F1 backcross populations；
D) from the BC3Selection is totally cleaved the individual plant AABBCC continuous bag sleevings of marking character with leaf margin in F1 backcross populations
It was selfed for 3 generations, cultivates the stable Rape Germplasm Resources AABBCC with marking character of totally cleaving, as stable has leaf margin
The rape self-mating system AABBCC for marking character of totally cleaving.
There is the stabilization obtained from above method seed selection the totally cleave rape self-mating system of marking character of leaf margin to enter
Row it is described with leaf margin totally cleave marking character rape sterile line seed selection seed selection flow as shown in figure 1, including as follows
Step：
(1) selection is using the sterile plant AACC of the excellent rape sterile line of the production as female parent, with having for the stabilization
Leaf margin totally cleave marking character rape self-mating system AABBCC for paternal hybrid obtain F1For AABCC, with F1It is selfed for plant bagging
Obtain F2For segregating population, from the F2For obtaining totally cleaving the sterile individual plant of marking character with leaf margin in segregating population
AABBCC；
(2) using obtain in step (1) with leaf margin totally cleave marking character sterile individual plant AABBCC to be maternal, with institute
Stating stable there is the totally cleave rape self-mating system AABBCC of marking character of leaf margin to carry out backcrossing for male parent and obtain BC1F is returned group
Body；
(3) from the BC1In F backcross populations selection with leaf margin totally cleave marking character sterile individual plant AABBCC and life
Key rape restorer AACC test crosses in production, fertile rate is filtered out up to 100%, and the band that hybrid vigour is strong and Comprehensive Traits are good
Have leaf margin totally cleave marking character maternal strain for described marking character of totally cleaving with leaf margin rape sterile line
AABBCC。
Present embodiments provide using it is described with leaf margin totally cleave marking character rape sterile line AABBCC to be maternal,
Carried using described with the totally cleave rape self-mating system AABBCC of marking character of leaf margin for the generation of male parent continuous backcross 2 acquisition homozygosis
Leaf margin totally cleaves the rape sterile line AABBCC of marking character.
Present embodiments provide using it is described with leaf margin totally cleave marking character rape sterile line AABBCC to be maternal,
Hybridized using described with the totally cleave rape self-mating system AABBCC of marking character of leaf margin as male parent, obtain that to carry leaf margin complete
Split the rape sterile line homotype maintainer AABBCC of marking character.
The present embodiment additionally provide utilize it is described with leaf margin totally cleave marking character rape sterile line AABBCC with
Common blade profile cabbage type rape restorer AACC test crosses flow is as shown in Figure 3, described to be totally cleaved marking character with leaf margin
Rape sterile line AABBCC with leaf margin totally cleave marking character rape sterile line homotype maintainer AABBCC in isolated area
(1) crossbreed obtains the rape sterile line AABBCC of described marking character of being totally cleaved with leaf margin, the common blade profile in
Cabbage type rape restorer AACC is bred in isolated area (two) obtains the common blade profile cabbage type rape restorer AACC, so
Afterwards by it is described with leaf margin totally cleave marking character rape sterile line AABBCC and the common blade profile cabbage type rape it is extensive
It is AACC test crosses in isolated area (three) again, is obtaining the cross rape F for marking character of being totally cleaved with leaf margin1AABCC, should
Test cross F1For the fertile rates of AABCC up to 100%, it is available for Production of Large Fields to use.In above-mentioned production process；1. produced in sterile line breeding
During i.e. in isolated area (one), pull out all non-leaf margins and totally cleave the plant at quarter, prevent field last year leave it is other
Kind, which is emerged, or seed is impure causes to mix；2. in Production of Large Fields with kind being totally cleaved the cross rape of marking character with leaf margin
F1The production production of hybrid seeds during i.e. in isolated area (three), after sterile line is spent eventually, cut off all carry cabbage type rape in time
Restorer plant, the comprehensive of restorer cuts off during ensureing hybrid seeding, prevents hybrid seed to be contaminated with restorer, ensures life
The purity of production kind.Leaf margin is totally cleaved quarter sterile line plant all retain, prevent excessive mistake from having cut sterile line and having influenceed the production of hybrid seeds
Yield；3. on F1 Production of Large Fields, Seedling Stage differentiates true and false hybrid in time, is planted at quarter if appearance a small amount of non-leaf margin in field totally cleaves
Strain, non-leaf margin to be totally cleaved in time and carve plant and all pull out, totally cleaved if there is a large amount of (more than 20%) non-leaf margins and carve plant, then
DNA identifications are carried out in time, and whether identification has used pseudosperm.Prevent pseudostationary or pseudosperm from causing seriously by this two measures
Loss, and reduce thus caused Seed enterprises and the seed dispute of Rape-seed production person.
Embodiment 2
The rape sterile line breeding method of the described marking character of being totally cleaved with leaf margin of this implementation, specifically includes following step
Suddenly：
The stabilization with leaf margin totally cleave marking character rape selection flow as shown in Fig. 2 its is specific
Comprise the following steps：
A) using cabbage type rape (RS013-008) AACC as female parent, Ye plant Zi Yuan indian rorippa herbs are belonged to Shi word Hua Ke indian rorippa herbs
BB is that male parent carries out distant hybridization acquisition F1For ABC, with F1It is selfed for plant bagging and obtains F2For segregating population, from F2Generation separation
The individual plant AABBCC for marking character of being totally cleaved with leaf margin is obtained in colony；
B) using F2 segregating populations obtain with leaf margin totally cleave marking character individual plant AABBCC to be maternal, with Wild cabbage type
Rape (RS013-008) AACC is that male parent backcrossing obtains BC1F backcross populations；
C) from the BC1In F backcross populations selection with leaf margin totally cleave marking character individual plant AABBCC be it is maternal, with
Cabbage type rape new lines (RS013-008) AACC is the generation of male parent continuous backcross 2, obtains BC3F1 backcross populations；
D) from the BC3Selection is totally cleaved the individual plant AABBCC continuous bag sleevings of marking character with leaf margin in F1 backcross populations
It was selfed for 3 generations, cultivates the stable Rape Germplasm Resources AABBCC with marking character of totally cleaving, as stable has leaf margin
The rape self-mating system AABBCC for marking character of totally cleaving.
There is the stabilization obtained from above method seed selection the totally cleave rape self-mating system of marking character of leaf margin to enter
Row it is described with leaf margin totally cleave marking character rape sterile line seed selection, comprise the following steps：
(1) selection is using the sterile plant AACC of the excellent rape sterile line of the production as female parent, with having for the stabilization
Leaf margin totally cleave marking character rape self-mating system AABBCC for paternal hybrid obtain F1For AABCC, with F1It is selfed for plant bagging
Obtain F2For segregating population, from the F2For obtaining totally cleaving the sterile individual plant of marking character with leaf margin in segregating population
AABBCC；
(2) using obtain in step (1) with leaf margin totally cleave marking character sterile individual plant AABBCC to be maternal, with institute
Stating stable there is the totally cleave rape self-mating system AABBCC of marking character of leaf margin to carry out backcrossing for male parent and obtain BC1F is returned group
Body；
(3) from the BC1Selection is female with the totally cleave sterile individual plant AABBCC of marking character of leaf margin in F backcross populations
This, using the stabilization have leaf margin totally cleave marking character rape self-mating system AABBCC as the generation of male parent continuous backcross 2, from obtaining
Backcross population in selection totally cleaved the sterile individual plant AABBCC of marking character with leaf margin, be described to be totally cleaved with leaf margin
The rape sterile line AABBCC of marking character, by the rape sterile line of the described marking character of being totally cleaved with leaf margin of acquisition
Individual plant AABBCC continuous bag sleevings selfing 3, which is commissioned to train, educates, the rape sterile line of the described marking character of being totally cleaved with leaf margin of acquisition
AABBCC individual plants and the upper key rape restorer AACC test crosses of production, filter out fertile rate up to 100%, and hybrid vigour is by force and comprehensive
Close rape sterile line AABBCC of the good maternal strain of character for described marking character of being totally cleaved with leaf margin.
Embodiment 3
The rape sterile line breeding method of the described marking character of being totally cleaved with leaf margin of this implementation, specifically includes following step
Suddenly：
The stabilization with leaf margin totally cleave marking character rape selection flow as shown in Fig. 2 its is specific
Comprise the following steps：
A) using cabbage type rape (RS013-008) AACC as female parent, Ye plant Zi Yuan indian rorippa herbs are belonged to Shi word Hua Ke indian rorippa herbs
BB is that male parent carries out distant hybridization acquisition F1For ABC, with F1It is selfed for plant bagging and obtains F2For segregating population, from F2Generation separation
The individual plant AABBCC for marking character of being totally cleaved with leaf margin is obtained in colony；
B) using F2 segregating populations obtain with leaf margin totally cleave marking character individual plant AABBCC to be maternal, with Wild cabbage type
Rape (RS013-008) AACC is that male parent backcrossing obtains BC1F backcross populations；
C) from the BC1In F backcross populations selection with leaf margin totally cleave marking character individual plant AABBCC be it is maternal, with
Cabbage type rape new lines (RS013-008) AACC is the generation of male parent continuous backcross 2, obtains BC3F1 backcross populations；
D) from the BC3Selection is totally cleaved the individual plant AABBCC continuous bag sleevings of marking character with leaf margin in F1 backcross populations
It was selfed for 3 generations, cultivates the stable Rape Germplasm Resources AABBCC with marking character of totally cleaving, as stable has leaf margin
The rape self-mating system AABBCC for marking character of totally cleaving.
There is the stabilization obtained from above method seed selection the totally cleave rape self-mating system of marking character of leaf margin to enter
Row it is described with leaf margin totally cleave marking character rape sterile line seed selection, comprise the following steps：
(1) selection is using the sterile plant AACC of the excellent rape sterile line of the production as female parent, with having for the stabilization
Leaf margin totally cleave marking character rape self-mating system AABBCC for paternal hybrid obtain F1For AABCC, with F1It is selfed for plant bagging
Obtain F2For segregating population, from the F2For obtaining totally cleaving the sterile individual plant of marking character with leaf margin in segregating population
AABBCC；
(2) using obtain in step (1) with leaf margin totally cleave marking character sterile individual plant AABBCC to be maternal, with institute
Stating stable there is the totally cleave rape self-mating system AABBCC of marking character of leaf margin to carry out backcrossing for male parent and obtain BC1F is returned group
Body；
(3) from the BC1Selection is female with the totally cleave sterile individual plant AABBCC of marking character of leaf margin in F backcross populations
This, using the stabilization have leaf margin totally cleave marking character rape self-mating system AABBCC as the generation of male parent continuous backcross 2, from obtaining
Backcross population in selection totally cleaved the sterile individual plant AABBCC of marking character with leaf margin, be described to be totally cleaved with leaf margin
The rape sterile line AABBCC of marking character, the rape sterile line AABBCC cultivations of the marking character of being totally cleaved with leaf margin
Sterile individual plant as shown in figure 5, wherein left side leaf margin totally cleave marking character cytoplasmatic male rape sterile line be with
Leaf margin totally cleave marking character rape sterile line cultivate sterile individual plant.
The rape sterile line and cabbage type rape sterile line of the relatively more described marking character of being totally cleaved with leaf margin of embodiment 4
Character
The seed selection flow of described cabbage type rape sterile line breeding method is as shown in figure 4, it specifically comprises the following steps：
(1) selection is using the sterile plant AACC of the excellent rape sterile line of the production as female parent, with cabbage type rape new product
It is that (RS013-008) AACC is that paternal hybrid obtains F1For AACC, with F1It is selfed for plant bagging and obtains F2For segregating population, from
The F2For the sterile individual plant AACC that cabbage type rape is obtained in segregating population；
(2) using the sterile individual plant AACC of the cabbage type rape of acquisition in step (1) as female parent, with the cabbage type rape certainly
Friendship is that AACC carries out backcrossing acquisition BC for male parent1F backcross populations；
(3) from the BC1The sterile individual plant AACC that cabbage type rape is selected in F backcross populations is female parent, with the wild cabbage
Type rape self-mating system AACC is that male parent carries out the generation of continuous backcross 2, and cabbage type rape sterile line is selected from the backcross population obtained
Sterile individual plant AACC, as described cabbage type rape sterile line AACC, the cabbage type rape cytoplasmatic male rape of cultivation is not
Educate is sterile individual plant as shown in figure 5, wherein right side is the cabbage type rape cytoplasmatic male rape sterile line infertility individual plant cultivated.
Compare the rape sterile line and cabbage type rape sterile line character of described marking character of being totally cleaved with leaf margin, such as
Shown in Fig. 5, wherein rape sterile line of the left side for the marking character of being totally cleaved with leaf margin described in embodiment 3, right side is training
The cabbage type rape cytoplasmatic male rape sterile line educated, by comparing it can be found that carrying leaf margin described in the cultivation of embodiment 3
The rape sterile line character of marking character of totally cleaving is stable, and leaf margin, which totally cleaves, carves that character is deeper, and character is obvious, easily identification.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (8)
- A kind of 1. rape sterile line breeding method for marking character of being totally cleaved with leaf margin, it is characterised in that comprise the following steps：(1) selection to be to produce the sterile plant AACC of excellent rape sterile line as female parent, stably to be totally cleaved blaze with leaf margin The rape self-mating system AABBCC of memory shape is that paternal hybrid obtains F1For AABCC, with F1It is selfed for plant bagging and obtains F2Generation separation Colony, from the F2For obtaining totally cleaving the sterile individual plant AABBCC of marking character with leaf margin in segregating population；(2) using obtain in step (1) with leaf margin totally cleave marking character sterile individual plant AABBCC for female parent, with it is described surely It is fixed to carry out backcrossing with the totally cleave rape self-mating system AABBCC of marking character of leaf margin for male parent and obtain BC1F backcross populations；(3) from the BC1In F backcross populations selection with leaf margin totally cleave marking character sterile individual plant AABBCC be it is maternal, with The stabilization is male parent continuous backcross 2-3 generations with the totally cleave rape self-mating system AABBCC of marking character of leaf margin, from obtaining Backcross population in selection totally cleaved the sterile individual plant AABBCC of marking character with leaf margin, be described to be totally cleaved quarter with leaf margin The rape sterile line AABBCC of mark property.
- 2. the rape sterile line breeding method of marking character according to claim 1 of being totally cleaved with leaf margin, its feature exist In including described with the totally cleave rape sterile line AABBCC individual plants of marking character of leaf margin connecting what step (3) obtained Continuous bagging selfing 3 is commissioned to train the step of educating.
- 3. the rape sterile line breeding method of marking character according to claim 1 or 2 of being totally cleaved with leaf margin, its feature It is, includes the rape sterile line individual plant AABBCC for the described marking character of being totally cleaved with leaf margin for obtaining step (3) With producing upper key rape restorer AACC test crosses, fertile rate is filtered out up to 100%, and hybrid vigour is strong and Comprehensive Traits are good Maternal strain for described marking character of totally cleaving with leaf margin rape sterile line AABBCC.
- 4. the rape sterile line breeding method of marking character according to claim 3 of being totally cleaved with leaf margin, its feature exist In, using cabbage type rape AACC as female parent, using Shi word Hua Ke indian rorippa herbs category Ye Sheng indian rorippa herbs BB be male parent progress distant hybridization seed selection obtain Obtain the rape self-mating system AABBCC of the marking character of being totally cleaved with leaf margin of the stabilization.
- 5. the rape sterile line breeding method of marking character according to claim 4 of being totally cleaved with leaf margin, its feature exist In, the rape self-mating system AABBCC seed selection acquisitions by the following method of the marking character of being totally cleaved with leaf margin of the stabilization, bag Include following steps：A) using cabbage type rape AACC as female parent, Ye plant Zi Yuan indian rorippa herbs BB is belonged to using Shi word Hua Ke indian rorippa herbs and carry out remote edge as male parent Hybridization obtains F1For ABC, with F1It is selfed for plant bagging and obtains F2For segregating population, from F2Leaf is carried for being obtained in segregating population Edge totally cleaves the individual plant AABBCC of marking character；B) using F2 segregating populations obtain with leaf margin totally cleave marking character individual plant AABBCC to be maternal, with cabbage type rape AACC is that male parent backcrossing obtains BC1F backcross populations；C) from the BC1Selection is maternal with the totally cleave individual plant AABBCC of marking character of leaf margin in F backcross populations, with wild cabbage Type rape AACC is the generation of male parent continuous backcross 2, obtains BC3F1 backcross populations；D) from the BC3In F1 backcross populations selection with leaf margin totally cleave marking character individual plant AABBCC continuous bag sleevings selfing 3 In generation, the Rape Germplasm Resources AABBCC of stable marking character of being totally cleaved with leaf margin is cultivated, as stable carries leaf margin The rape self-mating system AABBCC for marking character of totally cleaving.
- 6. it is a kind of it is according to claim 1 with leaf margin totally cleave marking character rape sterile line application, its feature Be, using it is described with leaf margin totally cleave marking character rape sterile line AABBCC and common blade profile cabbage type rape it is extensive It is AACC test crosses again, obtains the cross rape AABCC for marking character of being totally cleaved with leaf margin.
- 7. it is a kind of it is according to claim 1 with leaf margin totally cleave marking character rape sterile line application, its feature Be, using it is described with leaf margin totally cleave marking character rape sterile line AABBCC to be maternal, leaf margin is carried with described The rape self-mating system AABBCC for marking character of totally cleaving is that male parent is hybridized, and obtains the oil for marking character of being totally cleaved with leaf margin Dish sterile line homotype maintainer AABBCC.
- 8. it is a kind of it is according to claim 1 with leaf margin totally cleave marking character rape sterile line application, its feature Be, using it is described with leaf margin totally cleave marking character rape sterile line AABBCC to be maternal, leaf margin is carried with described The rape self-mating system AABBCC for marking character of totally cleaving is to obtain in the generation of male parent continuous backcross 2 homozygous to totally cleave blaze memory with leaf margin The rape sterile line AABBCC of shape.
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| CN108950052A (en) * | 2018-08-17 | 2018-12-07 | 中国农业科学院油料作物研究所 | With the InDel label of cabbage type rape floral leaf gene linkage and application |
| CN112941227B (en) * | 2021-03-30 | 2023-02-17 | 江西省农业科学院作物研究所 | Molecular marker for detecting rape leaf margin deep-etching marker character and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1559185A (en) * | 2004-02-24 | 2005-01-05 | 北京市农林科学院 | Method for selectively culturig with cytoplasmic male sterile line and its hybridized combination |
| CN1613296A (en) * | 2004-07-30 | 2005-05-11 | 镇江瑞繁农艺有限公司 | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period |
-
2016
- 2016-01-29 CN CN201610065700.8A patent/CN105706901B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1559185A (en) * | 2004-02-24 | 2005-01-05 | 北京市农林科学院 | Method for selectively culturig with cytoplasmic male sterile line and its hybridized combination |
| CN1613296A (en) * | 2004-07-30 | 2005-05-11 | 镇江瑞繁农艺有限公司 | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period |
Non-Patent Citations (4)
| Title |
|---|
| 油菜×蔊菜远缘杂交创新油菜种质资源研究;戴兴临等;《植物遗传资源学报》;20050728;第6卷(第2期);第242-244页 * |
| 甘蓝型油菜几个苗期形态标记的遗传;涂金星等;《华中农业大学学报》;20010831;第20卷(第4期);第318-320页 * |
| 甘蓝型油菜叶缘裂刻性状特征及其遗传分析;涂玉琴等;《中国油料作物学报》;20131031;第93-94页摘要，第1.1、1.4节，第95页 3讨论 第1段第9-17行 * |
| 甘蓝型油菜形态标记性状研究进展;吴新杰等;《农艺科学》;20051130;第21卷(第12期);第128-131页 * |
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