CN1613296A - Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period - Google Patents
Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period Download PDFInfo
- Publication number
- CN1613296A CN1613296A CN 200410041588 CN200410041588A CN1613296A CN 1613296 A CN1613296 A CN 1613296A CN 200410041588 CN200410041588 CN 200410041588 CN 200410041588 A CN200410041588 A CN 200410041588A CN 1613296 A CN1613296 A CN 1613296A
- Authority
- CN
- China
- Prior art keywords
- seedling
- line
- type rape
- yellow
- hybrid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
A process for recognizing and rejecting the sterile plant of crossed rape by use of seedling's genetic marker includes such steps as crossing between yellow-seedling small Chinese cabbage and cabbage-type rape to obtain the selfing line of yellow-seedling cabbage-type rape, crossing with the sterile plant in cytoplasmic sterile line of cabbage-type rape, backcrossing to obtain the sterile line and maintenance line of cabbage-type rape with yellow-seedling genetic marker, screening the strong-dominance restoring line, crossing to obtain seeds, sowing, and rejecting the yellow-seedling plants.
Description
Technical field
The present invention relates to a kind of method of utilizing genetic marker identification purity of hybrid and rejecting pseudostationary, especially about differentiating the seedling stage of the sterile strain of cabbage type rape hybrid and rejecting.
Background technology
Cross-bred rape breeding approach mainly contains and utilizes nuclear male sterility and cytoplasmic male sterility.Nuclear male sterility, wherein half is for educating, and half is sterile, like this when hybrid seeding, pull out wherein half educated strain in the early flowering season, as pulls out untimelyly or unclean, will produce brother and sister and hand over, and forms impalpable pseudostationary.Because the educated strain of pulling out half, lean on manually the early flowering season by the strain inspection, will all can educate simultaneously after strain pulls out, also need spending all of having opened in the sterile strain removed.This method amount of labour used is bigger, and breeding cost is higher.The major defect of cytoplasmic male sterility is, sterility is thorough inadequately, to responsive to temperature, is easy to generate the micro mist selfing and solid, also can form impalpable pseudostationary.So the cross-bred rape seed purity is difficult to improve at present, it only is 86% that national standard requires, and this gives and produces actual making troubles, and after planting can cause output to be affected because of there being a considerable amount of pseudostationaries.Cross-bred rape purity discrimination method mainly contains three kinds at present: strange land plantation evaluation, isodynamic enzyme and Molecular Identification.Isodynamic enzyme and Molecular Identification not only require to possess certain operative skill and experience, and instrument and equipment and biochemical reagents preferably will be arranged, and it is big, at the experimental stage mostly that technology is popularized difficulty, uses difficulty in the production.The strange land planting method then is a main method at present commonly used, and specification requirement is lower, but that shortcoming is required qualification time is longer relatively, obtains the result and often lags behind the seed sales behavior, and is not only time-consuming, costly, and restricted sale greatly.Simultaneously, even identify the existence of pseudostationary, also have no idea in the production pseudostationary is rejected.
For this reason, people begin to utilize genetic marker that true and false hybrid is carried out EARLY RECOGNITION, disclose a kind of wax powder-free genetic marker of utilizing as Chinese patent 01108531.2 and discerned true and false hybrid method, it comprises 1), the commentaries on classics seed selection of wax powder-free mark maintenance line, male sterile line or recovery system.2), utilizing the male sterile line or the recovery of carrying the wax powder-free genetic marker is the combination of seed selection advantage cross-bred rape; 3), when the advantage combination production of hybrid seeds, separate father and mother originally, reject non-parent's strain according to mark.Behind the results production of hybrid seeds district seed, according to wax powder-free genetic marker Rapid identification hybrid rate is arranged; 4), in the production application of F1 crossbreed, reject the selfing strain of male sterile line micro mist and other pseudostationary strains, thereby guarantee the germplasm safety of cross-bred rape in the seedling phase.This method though a kind of means that current cabbage type rape hybrid is rejected sterile strain that solve are provided, make it just can discern true and false hybrid from rape seedling, thereby just pull out pseudostationary when thinning.But the genetic marker of this method (blade has wax powder-free) is actual and not obvious, still difficult identification during the land for growing field crops produces, and therefore not only inconvenience is still felt in practical operation, and it is also relatively large carefully to discern the amount of labour used, but also can still retain pseudostationary because of discerning difficulty.
Farmers' Daily's article on August 16th, 2003 " new technology causes seed purity and detects revolution ", report Zhejiang University adopts the albefaction seedling to discern straight pseudostationary.Though this method mark is obvious, be easy to identification, its recognition time is short, only be just to show this genetic character before the 2-3 leaf, so still exist because of seedling is little, the time is short, easily carelessness, difficulty is thoroughly removed sterile strain in the land for growing field crops produces, so the land for growing field crops still has sterile strain pseudostationary to exist.
Therefore present genetic marker is discerned true and false hybrid, all there is defective in various degree, not difficult identification, in actual production, be difficult to grasp, it is exactly the proterties instability, be subject to environmental influence, some also needs the later stage could discern (as cauline leaf, pattern, fertility etc.), and some genetic character is also relevant with bad economical character.So be not to cause being difficult in breeding directly using, be exactly that to use difficulty in the actual production big, cause present cross-bred rape seed purity still to be difficult to improve, differentiate in early days and reject the pseudostationary difficulty, influence output, therefore still have worth improved place.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned prior art, a kind of long period that has in seedling stage is provided, can utilize the effective fast identification of genetic marker in seedling stage and reject the method for the sterile strain of cross-bred rape, guarantee that the hybrid purity of field cultivation plant reaches 100%.
The object of the invention realizes that at first the yellow seedling a variety of Chinese cabbage that obviously can discern genetic marker seedling stage that has by having selected to cabbage type rape hybridization transformation, is bred stable yellow seedling cabbage type rape inbred line (naming butter No.); The yellow seedling cabbage type rape inbred line that to breed again, with the sterile strain in the cabbage type rape cytoplasmic male sterile line, by hybridizing, backcrossing, transformation becomes to have the cabbage type rape male sterile line and the maintenance line of yellow seedling genetic marker; With this male sterile line (naming A of butter) preparing hybrid make up hybrid seed.Thereby make first-filial generation sow seedling stage, just can fast, intuitively identify purity of hybrid and reject the pseudostationary strain, guarantee that the hybrid purity of field cultivation plant reaches 100%.Specifically, the present invention utilizes genetic marker identification in seedling stage and rejects the method for the sterile strain of cross-bred rape, it is characterized in that to have seedling stage the yellow seedling a variety of Chinese cabbage that obviously can discern genetic marker,, breed stable yellow seedling cabbage type rape inbred line to cabbage type rape hybridization transformation; The yellow seedling cabbage type rape inbred line that to breed again, with the sterile strain in the cabbage type rape cytoplasmic male sterile line, by hybridizing, backcrossing, transformation becomes to have the cabbage type rape male sterile line and the maintenance line of yellow seedling genetic marker; Survey with this male sterile line again and join the strong advantage of screening and recover system, and with its preparing hybrid make up hybrid seed, after planting pull out and be yellow seedling plant in seedling stage (before 6 leaves).
Because the hybridization of a variety of Chinese cabbage and cabbage type rape belongs to distant hybridization, a variety of Chinese cabbage sexual cell chromosome number is 10, and is bigger with cabbage type rape sexual cell chromosome number 19 difference, so both cross compatibilities are relatively poor, ripening rate is lower, and the infertile phenomenon of selfing can appear in the offspring.Therefore, a kind of better method of the present invention is in flowering stage, adopts many strains pollen mixed pollination, to overcome the infertile phenomenon of distant hybrid progeny selfing, improves offspring plant ripening rate.From its offspring, select the good yellow seedling cabbage type rape plant of comprehensive proterties later on, until breeding the stable strain of proterties.
The present invention is by seed selection yellow seedling a variety of Chinese cabbage that has the long period (before 6 leaves) obvious leaf color marker seedling stage by recessive gene control, the hybridization transformation is on cabbage type rape cytoplasmic male sterile line, breed and have recessive yellow seedling genetic marker in seedling stage and (be yellow seedling before 6 leaves, whole strain changes green behind 6 leaves, do not influence the economical character in male sterile line later stage, do not influence its vitality and numerous yet, hybrid seed yield and hybrid vigour) cabbage type rape cytoplasmic male sterile line, use its preparing hybrid seed again, reaching can be according to yellow seedling mark in seedling stage, during the seedbed, can conveniently identify hybrid purity to the first-filial generation seed of being prepared, and pull out the false hybrid plant (the sterile selfing of matter or examine sterile brother and sister hand over " Huang Miao " then appears), the land for growing field crops hybrid purity is significantly improved, purity can reach 100%, guarantee that hybrid vigour is not fully exerted, guarantee the cross-bred rape stable yields, high yield.The inventive method has than prior art: " yellow seedlings " seedling stage presentation time longer, and true and false hybrid difference is obvious, identity is strong, can both conveniently discern and pull out sterile pseudostationary the field ordinary people, not influenced by quality of the workforce, even in addition final singling has been a kind of routine techniques in China's rape production, adopt traditionally, therefore can carry out in conjunction with the agronomy operation, and not need to drop in addition the labour, be better than other genetic character identification greatly.Secondly, the inventive method has been created " dual " and has efficiently been arranged false technical system (can realize dual row's vacation in breeding field, seed farm and land for growing field crops).Besides, " Huang Miao " genetic marker is the qualitative character by recessive gene control, and expression is stable not affected by environment, do not have bad economical character again and follow, and be the method for guaranteeing cabbage type rape hybrid seed quality safety.In addition, also can when the preparing hybrid kind, can reject impure male sterile line parent, improve seed production purity according to yellow seedling mark property.Overcome the crossbreed of being prepared, can not fast, effectively identify its hybrid purity and reject the deficiency of false hybrid plant in seedling stage with other type male sterile line, especially can be obviously, the Rapid identification hybrid seed purity, and thorough removing pseudostationary plant wherein.
Description of drawings
Accompanying drawing is schemed general system for the inventive method hybridization.
Embodiment
Embodiment: referring to accompanying drawing.
1, the seed selection of yellow seedling cabbage type rape inbred line (the conventional kind)
The a variety of Chinese cabbage that selection has yellow seedling characteristic is a male parent, carries out distant hybridization with the sterile strain of cabbage type rape (No. 2, Qin's oil), and results obtain the F1 seed.With the F1 planting seed, the infertile phenomenon of selfing (have pollen strain and WUHUAFEN strain, have pollen strain meeting selfing shaky) appears when blooming, and adopt F1 many strains of generation pollen to carry out mixed pollination, improve ripening rate, obtain the F2 seed.With the F2 planting seed, separate among the offspring again, select proterties, and have the individual plant of yellow seedling genetic marker, carry out selfing, selected selfing, breed stable yellow seedling cabbage type rape inbred line (naming " No. one, butter ") through 4 years near cabbage type rape at it.
2, have the seed selection of the cabbage type rape cytoplasmic male sterile line of yellow seedling genetic marker
" No. one, the butter " that form with seed selection is male parent, with sterile strain hybridization in cabbage type rape (the rather assorted No. 3) cytoplasmic male sterile line, in its segregative generation, pressing paternal character (as far as possible as male parent) selects sterile strain for maternal, use " No. one, butter " to carry out pairing backcrossing for the samsara male parent more continuously, through the selection of backcrossing of 5 generations, it is stable to obtain proterties, comprehensive proterties is better, and has the cabbage type rape cytoplasmic male sterile line " A of butter " of yellow seedling genetic marker.Annual when backcrossing with " No. one, butter " also continuous selfing purifying, promptly get maintenance line " B of butter ".
3, preparing hybrid combination
Carry out test cross with the cabbage type rape male sterile line that has yellow seedling genetic marker " A of butter " and a plurality of cabbage type rape inbred lines, filter out strong advantage and recover system, recovering with strong advantage is that male parent and this male sterile line make good hybrid combination again, produce hybrid seed, produce for the land for growing field crops and use.
Claims (2)
1, a kind ofly utilizes genetic marker identification in seedling stage and reject the method for the sterile strain of cross-bred rape, it is characterized in that the yellow seedling a variety of Chinese cabbage that obviously can discern genetic marker,, breed stable yellow seedling cabbage type rape inbred line to cabbage type rape hybridization transformation with having seedling stage; The yellow seedling cabbage type rape inbred line that to breed again, with the sterile strain in the cabbage type rape cytoplasmic male sterile line, by hybridizing, backcrossing, transformation becomes to have the cabbage type rape male sterile line and the maintenance line of yellow seedling genetic marker; Survey with this male sterile line again and join the strong system of recovering of screening, and with its preparing hybrid make up hybrid seed, after planting pull out and be yellow seedling plant in seedling stage (before 6 leaves).
2, according to the described method of utilizing the sterile strain of genetic marker identification cross-bred rape in seedling stage of claim 1, it is characterized in that adopting in the inbred line breeding many strains pollen mixed pollination in the filial generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410041588 CN1613296A (en) | 2004-07-30 | 2004-07-30 | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410041588 CN1613296A (en) | 2004-07-30 | 2004-07-30 | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1613296A true CN1613296A (en) | 2005-05-11 |
Family
ID=34763710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410041588 Pending CN1613296A (en) | 2004-07-30 | 2004-07-30 | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1613296A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706901A (en) * | 2016-01-29 | 2016-06-29 | 江西省农业科学院作物研究所 | Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application |
| CN108739327A (en) * | 2018-06-19 | 2018-11-06 | 安徽省农业科学院园艺研究所 | A kind of chilli kernel male sterile dual purpose lines producing method for seed |
| CN110235774A (en) * | 2019-03-18 | 2019-09-17 | 长沙丰景大地农业科技有限公司 | A method of cultivating wax powder-free cabbage type rape tender flower stalk |
| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
-
2004
- 2004-07-30 CN CN 200410041588 patent/CN1613296A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706901A (en) * | 2016-01-29 | 2016-06-29 | 江西省农业科学院作物研究所 | Rape sterile line with leaf margin holoblastically-carved marker characters and breeding method and application |
| CN105706901B (en) * | 2016-01-29 | 2017-12-19 | 江西省农业科学院作物研究所 | With leaf margin totally cleave marking character rape sterile line and selection and application |
| CN108739327A (en) * | 2018-06-19 | 2018-11-06 | 安徽省农业科学院园艺研究所 | A kind of chilli kernel male sterile dual purpose lines producing method for seed |
| CN108739327B (en) * | 2018-06-19 | 2020-05-29 | 安徽省农业科学院园艺研究所 | Seed production method of capsicum annuum nuclear male sterile dual-purpose line |
| CN110235774A (en) * | 2019-03-18 | 2019-09-17 | 长沙丰景大地农业科技有限公司 | A method of cultivating wax powder-free cabbage type rape tender flower stalk |
| CN110235774B (en) * | 2019-03-18 | 2022-08-19 | 长沙丰景大地农业科技有限公司 | Method for cultivating wax powder-free cabbage type rape bolts |
| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106069720A (en) | Brassica campestris L dihaploid induction system's selection-breeding cabbage type rape variety and the method for material | |
| CN103875462A (en) | Triple cross wheat breeding technical method | |
| CN103283587B (en) | Selective breeding and propagating method of ecological type sterile line of yellow-sprout capsicum | |
| CN106069719A (en) | The method of Brassica campestris L inheritance stability colony is created in the scale of Brassica campestris L dihaploid induction system | |
| EP0921720B1 (en) | Aphid resistance in composites | |
| CN1613296A (en) | Method for selecting and removing sterilic hybrid rapes by genetic marks in seedling period | |
| CN108651273B (en) | Method for breeding salt-tolerant lagerstroemia indica leaves by polymerization based on gene detection | |
| CN106035068A (en) | Method for breeding leaf mustard type rape varieties and materials by rape double haploid inducing line | |
| CN105613256A (en) | Watermelon hybridization breeding and hybrid identification method | |
| US5977443A (en) | Aphid resistance in composites | |
| CN1020390C (en) | Technology for breeding high-yield and quality radish by hybridisation | |
| CN104170716A (en) | Seed selection method of high stigma exsertion rate japonica two-line sterile line | |
| CN107593435B (en) | The identification of strong cold-resistant Chinese cabbage type winter rape study on temperature sensitive male sterility mutant and screening technique | |
| CN111512959A (en) | Hybrid rape breeding method capable of early identifying seed purity | |
| CADIC | Breeding for ever-red barberries (Berberis spp.) | |
| CN108633726B (en) | Rapid identification and seed reservation method for bitter gourd all-female plants | |
| CN106035069A (en) | Breeding method for hybrid rice two-line sterility line with albino ear lemmas | |
| CN106069721A (en) | Brassica campestris L dihaploid induction system's selection-breeding turnip type rape kind and the method for material | |
| CN100364385C (en) | Supergrade hybrid rice breeding parent matching method | |
| CN105671039A (en) | Molecular marker indel15-1 of soybean early-flowering-season major QTL (quantitative trait locus) and application thereof | |
| CN100393197C (en) | Method for producing hybrid of celery and seedling-stage purity appraising method | |
| CN114532216B (en) | Breeding method of multi-marker dual-purpose hybrid rice sterile line | |
| JP2002095352A (en) | Tomato graft seedling and tomato for rootstock to be used for the seedling | |
| CN108651268A (en) | A kind of selection of balsam pear gynoecy system | |
| CN107318642B (en) | A kind of cotton cells matter sterile line breeding high efficiency seed method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |