CN100393197C - Method for producing hybrid of celery and seedling-stage purity appraising method - Google Patents
Method for producing hybrid of celery and seedling-stage purity appraising method Download PDFInfo
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- CN100393197C CN100393197C CNB2005100865572A CN200510086557A CN100393197C CN 100393197 C CN100393197 C CN 100393197C CN B2005100865572 A CNB2005100865572 A CN B2005100865572A CN 200510086557 A CN200510086557 A CN 200510086557A CN 100393197 C CN100393197 C CN 100393197C
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Abstract
The present invention relates to a method for producing hybrid seeds of celery. The present invention takes parent materials of yellowing celery as the female parent and parent materials of normally green celery as the male parent to carry out hybridization so as to produce the hybrid seed. The yellowing character of the present invention is inherited stably. The present invention is not influenced by climate, conditions of cultivation, etc. (The yellowing character is obviously different from the light green character of the celery, which is influenced by environment.). False crossbreeds can be stably identified from the hybrid population. The result of appraising purity of the hybrid seeds is reliable, fast and accurate so that the period of appraising purity is shortened three months. The cost of appraising purity is reduced 90%. The yellowing false hybrid seed can be extracted in the seedling stage (after leaves unfold) in the process of production. The present invention can reduce the economic loss caused by the purity of the hybrid seeds.
Description
Technical field
The present invention relates to production and the purity authentication method of a kind of vegetable crop crossbreed.
Background technology
Celery is at the cultivation history of the existing two thousand years of China, and all generally there is cultivation all parts of the country at present, is to be subjected to one of popular vegetable that China consumer likes deeply.The adaptability of celery is very wide, but join protection ground and almost annually cultivating supply of different ecological condition.Breeding lacks and systematically studies and utilize but China is to celery.Existing celery breeding work mainly is to utilize original landrace to purificate and rejuvenate or utilize the offspring of the hybridization of abiogenesis between the celery different cultivars to select, and breeds new kind, but these breeding researches and to utilize work be very limited.This and the status of celery in China's vegetables production and supply are extremely disproportionate.
The kind of using in China's celery production at present all is conventional varieties, and this celery mainly is that landrace are main, and celery mainly is the kind of introducing from external (based on the U.S.), as texts and pictures draw, Utah series etc.Domesticly also do not carry out heterotic research of celery and utilization, had a strong impact on China's celery and produced and its international competitiveness.Because the celery floral organ is little, the single fruit seed is few, is again the cross pollination vegetables, can't produce crossbreed with artificial emasculation pollination's method.Indivedual countries (Holland etc.) have the male sterile of utilizing to carry out few report that the celery hybrid produces in the world, but because celery male sterile genus anas male sterile type (GMS), to dial educated strain when producing hybrid in the field except that 50%, this is difficult in time dial up hill and dale remove in the actual production of hybrid seeds, can not guarantee the purity of celery hybrid.So at present domestic and international celery kind is still based on conventional variety.
Utilizing the male sterile line production first generation of hybrid is at present domestic and international unique celery hybrid production approach.But the selective breeding celery male sterile line cycle is long, and technology is numerous and diverse, but also exist above-mentioned be difficult in time dialling up hill and dale remove 50% and can educate strain and make problems such as hybrid purity can not guarantee.Utilize the celery hybrid of male sterile line production also can't just carry out the evaluation of purity simultaneously in seedling stage.
Summary of the invention
(1), the technical problem that will solve
The purpose of this invention is to provide and a kind ofly can set up a kind of new celery hybrid production method by the present invention, set up simultaneously fast, accurately, good reproducibility, the impurity-removing method in seedling stage that celery hybrid purity authenticate technology that technology is simple to operation and field produce.
(2), technical scheme
The present invention uses Huang 9701 for maternal, and normal green celery parent material is that male parent is hybridized the production crossbreed.
Of the present invention yellow 9701, its cotyledon and true leaf are yellow.
The present invention when hybridization father and mother this plant by 1: 3～4 ratios, from the female parent that has the yellow proterties hybrid seed of gathering.
The purity authentication method of a kind of celery hybrid of the present invention, utilizing the yellow character pair crossbreed of celery cotyledon and true leaf to carry out purity identifies, launch or seedling carries out purity when 2-3 sheet true leaf occurring and identifies at cotyledon, cotyledon and true leaf for yellow be pseudostationary.
Concrete seed selection process is as follows:
Occur 2 strain yellow mutant strains among " 9816 celery " inbred line self progeny, select yellow strain selfing, obtain the 9816 individual plants system of whole yellows, selfing obtains " yellow 9816 celeries " again, with it is female parent, draws 9701 to be paternal hybrid with the normal celery inbred line of green texts and pictures, F
1All be normal green strain, F
1Selfing separates the normal green strain of acquisition again: the yellow strain is 3: 1, selects yellow strain wherein to make female parent, draws 9701 to backcross with texts and pictures, F
1All be normal green strain, backcross progeny selfing (BC
1F
2) separate obtaining normal green strain: the yellow strain is 3: 1, selects wherein yellow to involve 3 generations of continuous individual plant selfing in a criminal case and obtains yellow 9701 inbred lines.Except that yellow, other proterties of yellow 9701 are near " texts and pictures draw 9701 ".
(3), beneficial effect
The celery kind first generation of hybrid that is applied as of the technology of the present invention melts the new approach of having warded off, yellow character inheritance among the present invention is stable, its performance is not subjected to the influence (light green color proterties affected by environment has difference significantly in this and the celery) of weather, cultivation condition etc., make pseudostationary stably from hybrid population, differentiate, the hybrid purity qualification result is reliable, quick, accurate, make the purity assay period shorten 3 months, the purity appraisal cost has reduced by 90%; The present invention also can promptly dial pseudostationary in seedling stage in the production, reduce the economic loss that causes because of hybrid purity, so can make the celery seed industry obtain huge economic benefit and social benefit except that yellow.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Utilize " yellow 9816 inbred lines " and " yellow 9701 inbred lines " the spring in 2004,30 hybrid combinations have been prepared with leaf look normal 15 parts of green celery inbred lines respectively, carried out varietal yield test autumn and winter in 2004 in the greenhouse, therefrom filter out 4 good hybrid combinations, further produce the experiment well afoot.
Embodiment 1
(solid, petiole is thick, and is tender and crisp, quality better, and anti-bolting, more heat-resisting, main breed " texts and pictures draw " was high by 16% during production ratio was produced.)
Hybrid seed production is divided into parental seed production and hybrid seed is produced two parts:
1, parental seed production technology
(1) maternal yellow 9816 breeding
Must adopt into strain seed collecting method and collect seed, and the parent is carried out strict selection and superseded.When spontaneous pollination is collected seed, in maternal multiplication plot, with interior other celery kinds must not be arranged all at 2000 meters.
(2) the male parent texts and pictures draw the breeding of 9701 inbred lines
The Seed Collecting Methods of male parent is identical with common celery kind seed collecting method.Isolate to wait and require with maternal identical.
2, large rich No. 1 breeding of the first generation of hybrid
(1) isolate: hybridization parents Huang 9816, texts and pictures draw 9701 to isolate more than 1500 meters naturally with other celery kinds.
(2) this ratio of father and mother: the male parent texts and pictures draw 9701 with maternal yellow 9816 planting proportions of yellow be 1: 4, i.e. the maternal 1 row male parent of planting of 4 row yellows.
(3) florescence is adjusted and collects seed: the prematurity parent extracts main flower a kind of sedge, makes parents' flower synchronization, and the hybridization of giving free rein to is dialled before the florescence seed prematurity later and removed male parent.From yellow maternal yellow 9816 hybrid seed of gathering.
(4) impurity elimination: according to this proterties of father and mother of breeding, before blooming, in time remove assorted strain, particularly before blooming, remove the non-yellow strain that may mix in the female parent.
(5) purity is identified: identify purity (hybrid rate) in indoor seeding as early as possible behind the seed of gathering.
Embodiment 2:
(plant height, solid, petiole is slightly grown, quality better, anti-bolting, warm tolerance is better, and " the real celery of Jin Nan " of cultivation was high by 13% during production ratio was produced.)
Hybrid seed production is divided into parental seed production and hybrid seed is produced two parts:
1, parental seed production technology
(1) maternal yellow 9816 breeding
Must adopt into strain seed collecting method and collect seed, and the parent is carried out strict selection and superseded.When spontaneous pollination was collected seed, all must not there be other celery kinds maternal multiplication plot with interior for inherent 2000 meters.
(2) breeding of male parent Tianjin 9606 inbred lines
The Seed Collecting Methods of male parent is identical with common celery kind seed collecting method.Isolate to wait and require with maternal identical.
2, large rich No. 2 breedings of the first generation of hybrid
(1) isolate: hybridization parents Huang 9816, Tianjin 9606 isolate more than 1500 meters naturally with other celery kinds.
(2) this ratio of father and mother: male parent Tianjin 9606 is 1: 4 with maternal yellow 9816 planting proportions of yellow, i.e. the maternal 1 row male parent of planting of 4 row yellows.
(3) florescence is adjusted and collects seed: the prematurity parent extracts main flower a kind of sedge, makes parents' flower synchronization, and the hybridization of giving free rein to is dialled before the florescence seed prematurity later and removed male parent.From yellow maternal yellow 9816 hybrid seed of gathering.
(4) impurity elimination: according to this proterties of father and mother of breeding, before blooming, in time remove assorted strain, particularly before blooming, remove the non-yellow strain that may mix in the female parent.
(5) purity is identified: identify purity (hybrid rate) in indoor seeding as early as possible behind the seed of gathering down.
Embodiment 3
(plant height, hollow, the shallow white of petiole, tender and crisp, quality better, more anti-bolting, warm tolerance is better, kind volume increase 18% of the same type during production ratio is produced.)
Hybrid seed production is divided into parental seed production and hybrid seed is produced two parts:
1, parental seed production technology
(1) maternal yellow 9701 breeding
Must adopt into strain seed collecting method and collect seed, and the parent is carried out strict selection and superseded.When spontaneous pollination was collected seed, all must not there be other celery kinds maternal multiplication plot with interior for inherent 2000 meters.
(2) breeding of white 9807 inbred lines of male parent
The Seed Collecting Methods of male parent is identical with common celery kind seed collecting method.Isolate to wait and require with maternal identical.
2, large rich No. 3 breedings of the first generation of hybrid
(1) isolate: hybridization parents Huang 9701, white 9807 is isolated more than 1500 meters naturally with other celery kinds.
(2) this ratio of father and mother: male parent white 9807 is 1: 3 with maternal yellow 9701 planting proportions of yellow, i.e. the maternal 1 row male parent of planting of 3 row yellows.
(3) florescence is adjusted and collects seed: the prematurity parent extracts main flower a kind of sedge, makes parents' flower synchronization, and the hybridization of giving free rein to is dialled before the florescence seed prematurity later and removed male parent.From yellow maternal yellow 9701 hybrid seed of gathering.
(4) impurity elimination: according to this proterties of father and mother of breeding, before blooming, in time remove assorted strain, particularly before blooming, remove the non-yellow strain that may mix in the female parent.
(5) purity is identified: identify purity (hybrid rate) in indoor seeding as early as possible behind the seed of gathering down.Cotyledon and true leaf yellow person are pseudostationary.
Embodiment 4
(strain is short, and is solid, and petiole is short and thick, light green color, quality better, anti-bolting.)
Hybrid seed production is divided into parental seed production and hybrid seed is produced two parts:
1, parental seed production technology
(1) maternal yellow 9701 breeding
Must adopt into strain seed collecting method and collect seed, and the parent is carried out strict selection and superseded.Isolate in the canopy artificial supplementary pollination in insect protected and collect seed, hybridizing maternal multiplication plot all must not have other celery kinds with interior for inherent 2000 meters.
(2) breeding of male parent Cornell inbred line
The Seed Collecting Methods of male parent is identical with common celery kind seed collecting method, isolates to wait to require with maternal identical.
2, large rich No. 4 breedings of the first generation of hybrid
(1) isolate: hybridization parents and other celery kinds are isolated more than 1500 meters naturally.
(2) this ratio of father and mother: maternal yellow 9701 planting proportions of male parent Cornell and yellow are 1: 3, i.e. the maternal 1 row male parent of planting of 3 row yellows.
(3) florescence is adjusted and collects seed: the prematurity parent extracts main flower a kind of sedge, makes parents' flower synchronization, and the hybridization of giving free rein to is dialled before the florescence seed prematurity later and removed male parent.From yellow maternal yellow 9701 hybrid seed of gathering.
(4) impurity elimination: according to this proterties of father and mother of breeding, before blooming, in time remove assorted strain, particularly before blooming, remove the non-yellow strain that may mix in the female parent.
(5) purity is identified: identify purity (hybrid rate) in indoor seeding as early as possible behind the seed of gathering down.
Claims (5)
1. the production method of a celery hybrid is characterized in that, use yellow celery parent material yellow 9701 is maternal, and normal green celery parent material is that male parent is hybridized the production crossbreed; Wherein, yellow 9701 selection is: yellow 9816 being female parent, draw 9701 to be that male parent is hybridized and obtained F with normal green celery inbred line texts and pictures
1, F
1Carry out selfing, it is maternal to select self progeny's yellow strain to do, and draws 9701 to backcross with texts and pictures, and backcross progeny is selfing again, involves 3 generations of continuous individual plant selfing in a criminal case with the yellow that obtains and obtains yellow 9701.
2. production method as claimed in claim 1 is characterized in that, described yellow 9701, its cotyledon and true leaf are yellow.
3. production method as claimed in claim 1 is characterized in that, yellow 9701 during with normal green celery parent material hybridization, and this plants father and mother by 1: 3～4 ratios, from the female parent that has the yellow proterties hybrid seed of gathering.
4. the purity authentication method of a celery hybrid, it is characterized in that, the crossbreed that the described method of use claim 1 is produced after planting utilizes the yellow character pair crossbreed of celery cotyledon and true leaf to carry out the purity evaluation, and cotyledon and true leaf are pseudostationaries for yellow.
5. purity authentication method as claimed in claim 5 is characterized in that, carries out purity and identify when 2-3 sheet true leaf appears in cotyledon period or seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100865572A CN100393197C (en) | 2005-09-30 | 2005-09-30 | Method for producing hybrid of celery and seedling-stage purity appraising method |
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| CNB2005100865572A CN100393197C (en) | 2005-09-30 | 2005-09-30 | Method for producing hybrid of celery and seedling-stage purity appraising method |
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| CN100393197C true CN100393197C (en) | 2008-06-11 |
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| CNB2005100865572A Expired - Fee Related CN100393197C (en) | 2005-09-30 | 2005-09-30 | Method for producing hybrid of celery and seedling-stage purity appraising method |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP6815075B2 (en) * | 2015-10-15 | 2021-01-20 | 株式会社サカタのタネ | New spinach and its production method |
| CN110029189B (en) * | 2019-05-13 | 2020-09-01 | 中国农业大学 | Molecular marker coseparated with celery cytoplasmic male sterility gene and application |
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- 2005-09-30 CN CNB2005100865572A patent/CN100393197C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 美国文图拉西芹高效栽培技术. 谢陈国.中国农业信息快讯，第1期. 2002 |
| 美国文图拉西芹高效栽培技术. 谢陈国.中国农业信息快讯，第1期. 2002 * |
| 芹菜杂交技术初探. 张燕，卢长明，赵新彬.长江蔬菜，第11期. 1998 |
| 芹菜杂交技术初探. 张燕，卢长明，赵新彬.长江蔬菜，第11期. 1998 * |
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Granted publication date: 20080611 Termination date: 20120930 |