CN102870667A - Open type cross breeding method of hot pepper cytoplasmic male sterile line - Google Patents
Open type cross breeding method of hot pepper cytoplasmic male sterile line Download PDFInfo
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- CN102870667A CN102870667A CN2012103914617A CN201210391461A CN102870667A CN 102870667 A CN102870667 A CN 102870667A CN 2012103914617 A CN2012103914617 A CN 2012103914617A CN 201210391461 A CN201210391461 A CN 201210391461A CN 102870667 A CN102870667 A CN 102870667A
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Abstract
The invention provides an open type cross breeding method of a hot pepper cytoplasmic male sterile line. The method takes a cytoplasmic male sterile line as the female parent and a restoring line as the male parent and breeds the first generation of hybrid by an open type pollination technology in a planting base. According to the invention, the female parent is subjected to open type pollination in the planting base, so that the hot pepper cross breeding process is simplified, and the breeding cost of the first generation of hybrid is reduced so as to promote large-scale seed production of hybrid.
Description
Technical field
The invention belongs to agricultural product heterosis utilization technical field, be specifically related to a kind of open cross breeding method of capsicum cytoplasm male sterility line.
Background technology
Capsicum (chillies) belongs to Solanaceae, and 1 year or herbaceos perennial originate in South America torrid areas, and the end of the Ming Dynasty is imported the ground of Hunan, China Chu into, and the plantation in existing more than 300 year is historical in China.Capsicum is the very abundant vegetables of a kind of nutrition, in recent years, along with improving constantly of people's living standard, is more and more liked by consumers in general, and consumption figure, cultivated area increase year by year.Plant hybrid vigour is extensively utilized in agricultural production, the capsicum crossbreed is than conventional variety, have the advantages such as high yield, product are of fine quality, resistance, become gradually the first-selection at vast pepper planting family, the demand of crossbreed is also increasing, and the extensive cross breeding seed of capsicum has become the task of top priority.At present, artificial pollination in the normal employing gauze in the capsicum cross breeding seed, the method Chang Yin has the defective of following several respects and affects cross breeding seed efficient: at first, artificial pollination not only needs to build facility plastic greenhouse, buys pollination equipment in the gauze, and labour that need to be more, breeding cost is higher, seriously restricts the extensive production of hybrid seeds; Secondly, in the facilities environment, insect is few, circulation of air is poor, insect pollination and anemophily are less, and mainly by manually pollinating, and that production of hybrid seeds parent is bloomed is relatively concentrated, need a large amount of labours, only have the workman to be skilled in technique, could ensure pollination quality, if pollinate untimely or the pollination quantity not sufficient all easily cause the fruit drop phenomenon; At last, there are the microclimate environments such as illumination is weak, high humidity in the facilities environment, the blooming of capsicum, loose powder, fertilization and fruit growth etc. are all had certain impact, therefore be necessary to improve.
Summary of the invention
The technical problem that the present invention solves: the open cross breeding method that a kind of capsicum cytoplasm male sterility line is provided, in the planting base, female parent is carried out open pollination, simplify the capsicum crossbreed procedure of breeding, reduce first generation of hybrid breeding cost, thereby promote the extensive production of hybrid seeds of crossbreed.
The technical solution used in the present invention: a kind of open cross breeding method of capsicum cytoplasm male sterility line take cytoplasm male sterility line as maternal, is male parent to recover, and adopts open pollination technique to carry out first generation of hybrid breeding in the planting base.
Further, in same breeding base, can plant simultaneously a plurality of cytoplasm male sterility lines and a kind of restorer.
Further, described cytoplasm male sterility line and restorer are planted in same base, also breed restorer in the breeding crossbreed.
Further, described male parent is 1:2 with the field planting ratio of female parent, and male parent is interplanted between female parent, and wherein the maternal spacing with male parent of delegation is 50cm, and the maternal spacing with male parent of another row is 70-82cm, the circulation field planting.
Preferably, described male parent and maternal plantation density are every mu of 3060 strains, wherein maternal 2040 strains, and male parent 1020 strains, the florescence is carried out open pollination.
Preferably, described male parent and maternal plantation density are every mu of 3366 strains, wherein maternal 2244 strains, and male parent 1122 strains, the florescence is carried out open pollination.
The present invention's advantage compared with prior art:
1, saves the input of the aspects such as labour, breeding equipment, greatly reduce the breeding cost of the first generation of hybrid, can realize extensive breeding;
2, the microclimate environment that does not have gauze isolation, natural environment are conducive to fully the blooming of capsicum, loose powder and fertilization, improve breeding efficiency;
3, do not need artificial pollination, but the maternal plant rational close planted, thus improve crossbreed output;
4, the pollen of open pollination in most cases belongs to pollen mixture, is conducive to like this improve fruit-setting rate and the fruit quality of capsicum, thereby improves breeding efficiency.
Description of drawings
Fig. 1 is male parent of the present invention and maternal mode of planting structural representation.
Embodiment
Below in conjunction with accompanying drawing 1 a kind of embodiment of the present invention is described.
A kind of open cross breeding method of capsicum cytoplasm male sterility line take cytoplasm male sterility line as maternal, is male parent to recover, and adopts open pollination technique to carry out first generation of hybrid breeding in the planting base; In same breeding base, can plant simultaneously a plurality of cytoplasm male sterility lines and a kind of restorer, carry out simultaneously the breeding of a plurality of crossbreed, and can guarantee the purity of a plurality of crossbreed; Male parent is 1:2 with maternal field planting ratio, male parent interplant between female parent (as shown in Figure 1, wherein ♂ represent male parent, and ♀ represent maternal) wherein the female parent of delegation and the spacing of male parent be 50cm, the spacing of another row female parent and male parent is 70-82cm, the circulation field planting.Male parent and maternal plantation density are every mu of 3060-3366 strain, wherein maternal 2040-2244 strain, and male parent 1020-1122 strain, the florescence is carried out open pollination.
Technical scheme of the present invention mainly comprises following several key technologies: the one, and production of hybrid seeds base, at first, production of hybrid seeds base is chosen as preferably area of nature isolation condition, guarantees purity of hybrid; Secondly, this area's soil property is fertile, can fill with and can arrange, and guarantees that production of hybrid seeds parent grows normally, grows; At last, this production of hybrid seeds base vegetation is abundant, has abundant pollination medium, can guarantee abundant pollination like this; The 2nd, production of hybrid seeds parent, at first, take capsicum cytoplasmic sterile line (CMS) as maternal, recovery is male parent, can guarantee like this pollen source that production of hybrid seeds base is only, thereby guarantee purity of hybrid; Secondly, father, maternal necessary flower synchronization can be carried out the florescence adjusting by regulating the means such as sowing time; The 3rd, cropping pattern, Parent definite value ratio and density, at first, male parent must be interplanted in maternal middle, is conducive to so maternal abundant pollination, is advisable with the wide-and narrow-row interplanting; Secondly, rational father, maternal ratio, the too small female parent of ratio can not obtain sufficient pollen impact fertilization, and the excessive hybrid seed yield that affects is advisable with 1:2 with the comparison of male parent and female parent; At last, rational plantation density, density is too small can not to take full advantage of the soil, the excessive fertilization that is unfavorable for, affect seed production efficiency, male parent and maternal plantation density are every mu of 3060-3366 strain, wherein maternal 2040-2244 strain, male parent 1020-1122 strain, florescence is carried out open pollination, every mu can obtain crossbreed 25.6kg, and restorer selfed seed 10.5kg invests 1000 yuan altogether.The results showed, the crossbreed thousand kernel weight that this method obtains is 7.65g, and germination rate is 100%, and purity is 100%.
The present invention also can be used for production of hybrid seeds parent's breeding, and cytoplasm male sterility line and restorer are planted in same base, also can breed restorer in the breeding crossbreed; Cytoplasm male sterility line and maintainer are planted in same base, can breed simultaneously cytoplasm male sterility line and maintainer.
Above-described embodiment is preferred embodiment of the present invention, is not to limit the scope of the present invention, therefore all equivalences of being done with the described content of claim of the present invention change, all should be included within the claim scope of the present invention.
Claims (6)
1. the open cross breeding method of a capsicum cytoplasm male sterility line is characterized in that: take cytoplasm male sterility line as maternal, be male parent to recover, adopt open pollination technique to carry out first generation of hybrid breeding in the planting base.
2. the open cross breeding method of capsicum cytoplasm male sterility line according to claim 1 is characterized in that: in same breeding base, can plant simultaneously a plurality of cytoplasm male sterility lines and a kind of restorer.
3. the open cross breeding method of capsicum cytoplasm male sterility line according to claim 1 and 2, it is characterized in that: described cytoplasm male sterility line and restorer are planted in same base, also breed restorer in the breeding crossbreed.
4. the open cross breeding method of capsicum cytoplasm male sterility line according to claim 1 and 2, it is characterized in that: described male parent is 1:2 with the field planting ratio of female parent, male parent is interplanted between female parent, and wherein the maternal spacing with male parent of delegation is 50cm, the maternal spacing with male parent of another row is 70-82cm, the circulation field planting.
5. the open cross breeding method of capsicum cytoplasm male sterility line according to claim 4 is characterized in that: described male parent and maternal plantation density are every mu of 3060 strains, wherein maternal 2040 strains, and male parent 1020 strains, the florescence is carried out open pollination.
6. the open cross breeding method of capsicum cytoplasm male sterility line according to claim 4 is characterized in that: described male parent and maternal plantation density are every mu of 3366 strains, wherein maternal 2244 strains, and male parent 1122 strains, the florescence is carried out open pollination.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103914617A CN102870667A (en) | 2012-10-12 | 2012-10-12 | Open type cross breeding method of hot pepper cytoplasmic male sterile line |
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| CN2012103914617A CN102870667A (en) | 2012-10-12 | 2012-10-12 | Open type cross breeding method of hot pepper cytoplasmic male sterile line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912369A (en) * | 2017-02-13 | 2017-07-04 | 南通科技职业学院 | A kind of spring of male sterility capsicum, autumn " two-part " cross-breeding technology |
| CN114902956A (en) * | 2022-06-08 | 2022-08-16 | 三明市农业科学研究院 | Greenhouse hybridization seed production method for CMS three-line pepper |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397158A (en) * | 2002-08-14 | 2003-02-19 | 江苏省农业科学院蔬菜研究所 | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds |
| CN101707977A (en) * | 2009-12-23 | 2010-05-19 | 昆明理工大学 | Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line |
-
2012
- 2012-10-12 CN CN2012103914617A patent/CN102870667A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1397158A (en) * | 2002-08-14 | 2003-02-19 | 江苏省农业科学院蔬菜研究所 | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds |
| CN101707977A (en) * | 2009-12-23 | 2010-05-19 | 昆明理工大学 | Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line |
Non-Patent Citations (4)
| Title |
|---|
| 《中国辣椒》 20011231 王述彬等 "辣(甜)椒胞质雄性不育系杂优利用及制种技术研究" 第3-8页 1-6 , 第2期 * |
| 《安徽农业技术师范学院学报》 20011231 张子学等 "不同授粉方法对辣椒座果的影响" 第20-23页 1-6 第15卷, 第2期 * |
| 张子学等: ""不同授粉方法对辣椒座果的影响"", 《安徽农业技术师范学院学报》, vol. 15, no. 2, 31 December 2001 (2001-12-31), pages 20 - 23 * |
| 王述彬等: ""辣(甜)椒胞质雄性不育系杂优利用及制种技术研究"", 《中国辣椒》, no. 2, 31 December 2001 (2001-12-31), pages 3 - 8 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912369A (en) * | 2017-02-13 | 2017-07-04 | 南通科技职业学院 | A kind of spring of male sterility capsicum, autumn " two-part " cross-breeding technology |
| CN114902956A (en) * | 2022-06-08 | 2022-08-16 | 三明市农业科学研究院 | Greenhouse hybridization seed production method for CMS three-line pepper |
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Application publication date: 20130116 |