CN101707977A - Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line - Google Patents
Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line Download PDFInfo
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- CN101707977A CN101707977A CN200910163216A CN200910163216A CN101707977A CN 101707977 A CN101707977 A CN 101707977A CN 200910163216 A CN200910163216 A CN 200910163216A CN 200910163216 A CN200910163216 A CN 200910163216A CN 101707977 A CN101707977 A CN 101707977A
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 38
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 239000006002 Pepper Substances 0.000 title claims abstract description 8
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 8
- 235000017804 Piper guineense Nutrition 0.000 title claims abstract description 8
- 235000008184 Piper nigrum Nutrition 0.000 title claims abstract description 8
- 244000203593 Piper nigrum Species 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims abstract description 24
- 241000722363 Piper Species 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000010899 nucleation Methods 0.000 claims abstract description 14
- 238000013138 pruning Methods 0.000 claims abstract description 7
- 240000008574 Capsicum frutescens Species 0.000 claims description 43
- 239000001390 capsicum minimum Substances 0.000 claims description 29
- 206010021929 Infertility male Diseases 0.000 claims description 22
- 208000007466 Male Infertility Diseases 0.000 claims description 22
- 230000009977 dual effect Effects 0.000 claims description 22
- 238000011084 recovery Methods 0.000 claims description 13
- 230000012010 growth Effects 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005194 fractionation Methods 0.000 claims description 3
- 206010000210 abortion Diseases 0.000 claims description 2
- 231100000176 abortion Toxicity 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000003306 harvesting Methods 0.000 abstract description 3
- 241000758706 Piperaceae Species 0.000 abstract 1
- 238000010415 tidying Methods 0.000 abstract 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 4
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 4
- 230000010152 pollination Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 235000002568 Capsicum frutescens Nutrition 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a method for producing hybrid seeds by use of pepper genic male sterile line and restorer line. The method comprises the following steps: 1, planting a pepper genic male sterile AB line and a restorer line in an area or a greenhouse where pepper plants are capable of overwintering cultivation respectively, taking sterile plants of the genic male AB line as female parent and taking the restorer line as male parent to perform hybridization so as to produce hybrid seeds; 2, pruning aerial parts of the sterile plants and the restorer line plants after the harvest of hybrid seeds in winter and then performing overwintering cultivation; 3, pruning and tidying dormant buds of the overwintering plants after the dormant buds sprout next year, taking the sterile plants as the female parent and taking the restorer line as the male parent to perform hybridization during florescence so as to produce hybrid seeds; and 4, repeating a step 2 and a step 3 three to four times. The method can continually harvest pepper hybrid seeds in a plurality of years after seeding once in a year, saves labor and time for hybrid seed production, is low in cost and long in heterosis-holding time, capable of guaranteeing the yield and quality of hybrid peppers and convenient for commercial application, and has important significance for popularizing the technology of utilizing pepper heterosis.
Description
Technical field
The present invention relates to vegetables hybrid seeds technical field, particularly a kind of hybrid seeds method of utilizing the dual-purpose system of chilli kernel male sterile.
Background technology
The utilization of capsicum heterosis, hybrid vigor is to improve yield of hot pepper, improves quality, strengthens one of effective way of resistance.Domestic and international research shows, the amount of increase in production of capsicum first-filial generation is generally 30 ~ 50%, but capsicum all shows as continuously and yields positive results, and the florescence is long, flower disperses, so the difficulty of heterosis, hybrid vigor research and utilization is more much bigger than the concentrated crop that yields positive results of paddy rice, corn etc.Three decisive factors of heterosis utilization, the one, the power of heterosis, hybrid vigor, the 2nd, the height of hybrid cost, the 3rd, the difficulty or ease of production of hybrid seeds process.
The method of using at present the capsicum heterosis, hybrid vigor aborning mainly contains: utilize artificial emasculation pollination's direct combo method, and the production cost height of seed, purity is difficult to guarantee; Utilize genic male sterility dual purpose to do the production that female parent carries out the first-filial generation seed at present and begun to occur, but all need annual plantation once, genic male sterility dual purpose, recover system and all need numerous kind, increased the cost of the production of hybrid seeds; The parent is unavoidable variation and the degeneration that produces gene in the process of breeding, causes the reduction of heterosis, hybrid vigor, influences output, quality and the resistance of capsicum.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of method of carrying out hybrid seeding with chilli kernel male sterile system and recovery system is provided, this method can be sowed sustained yield hybrid seed for many years in 1 year, hybrid seeding saving of work and time, expense are low, the heterosis, hybrid vigor retention time is long, output, quality and the resistance of hybridization capsicum also can be protected, and are convenient to commercial applications, utilize the popularization of technology significant to the capsicum heterosis, hybrid vigor.
The technical scheme of producing method for seed of the present invention is: utilize the dual-purpose system of chilli kernel male sterile and recover the cross-breeding method that system survives the winter and regenerates, it is characterized in that the step of said method is:
A, production of hybrid seeds area or the booth that can survive the winter at pepper plant in 1 year, plant the dual-purpose system of chilli kernel male sterile respectively and recover system, florescence is removed the educated strain in the genic male sterility dual purpose, female parent is done in sterile strain with genic male sterility dual purpose, recover system and do male parent, carry out hybrid seeding, produce capsicum first-filial generation seed, be used for commerial growing;
B, sterile strain and the recovery that will gather in the crops in the genic male sterility dual purpose of hybrid seed are plant, carry out overwintering cultivation after the pruning of acrial part in the winter time;
C, next year prune and put in order after the plant sleeping bud after surviving the winter is sprouted, and do female parent at the florescence with male sterile line subsequently, and recovering is to do male parent to hybridize, and produces hybrid seed, is used for the capsicum commerial growing;
D, repetition B, C two steps 3 ~ 4 times.
The production of hybrid seeds area that the said pepper plant of the present invention can be survived the winter is meant winter minimum temperature 〉=15 ℃, the illumination abundance, and the water source is the area easily, in the area that meets above-mentioned condition, pepper plant can both be survived the winter smoothly, and can not freeze to death, can regerminate in 1 year, grow new plant.
Said genic male sterility dual purpose is an abortion genic male sterility dual purpose completely; Contain 50% educated strain in the genic male sterility dual purpose of plantation in 1 year in the A step, can educate strain at the florescence and all pull out, and do female parent with remaining male sterile plant again, recovering is to do male parent to carry out hybrid seeding, production first-filial generation pepper seed is used for commerial growing.
Sterile strain and the recovery that to gather in the crops the genic male sterility dual purpose of hybrid seed in the said B step are plant, carry out overwintering cultivation after the pruning of acrial part in the winter time, specific practice is: mid-November to early December, pepper plant is pruned, overground part fractionation 20 ~ 30cm height, above the plant of cutting covered, watered once in 20 ~ 30 days; After next year sprouts the plant sleeping bud after surviving the winter in the said C step, prune and put in order, its concrete grammar is: it is long to 10 ~ 15cm when high to wait pepper plant to sprout sprouting, stay 1 ~ 2 growth vigorous, well-developed sprouting bud, other sproutings that sprouted are all removed, after 30 ~ 45 days, select the lateral bud of 1 robust growth, remove other lateral bud, carry out the conventional cultivation management.
Genic male sterility dual purpose that the present invention uses and recovery system can be that existing various genic male sterility dual purpose and recovery are capsicum variety, and, take the existing cultivation management measure of corresponding strain to carry out field management according to different genic male sterility dual purposes and recovery system.
Compared with prior art, the present invention has following advantage or good effect:
The present invention is as long as be the parent the plantation dual-purpose system of chilli kernel male sterile and recovery in 1 year, can gather in the crops capsicum first-filial generation seed in continuous 4 ~ 5 years, than sow the parent and need carry out parent propagation every year every year, its advantage is: 1, saved 1 year and after sow, grow seedlings, labour's such as transplanting input; 2, saved 1 year and after annual plantation of each year parent's seed expense usefulness, reduce the input that the capsicum hybrid seed is produced; 3, assorted strains such as the educated strain in the genic male sterility dual purpose were thoroughly removed in 1 year, do not needed operations such as impurity elimination in 1 year, further reduced the purity of capsicum hybrid seed, guaranteed the quality of seed; 4, since 1 year, pepper plant is bloomed early, can prolong the production of hybrid seeds time, has improved the benefit of capsicum hybrid seeding; 5, sowing once utilizes for many years, and production of hybrid seeds place is relatively stable, and scale is controlled easily; 6, just need to use new parental seed in 4 ~ 5 years, reduced the algebraical sum scale of parent propagation, reduced the phenomenons such as variation, degeneration of parent material, output and the quality of hybridization capsicum are protected.In a word, hybrid seeding of the present invention makes that capsicum hybrid seeding cost is low, labour's less investment, and yield of hot pepper height, stay in grade are convenient to commercial applications, and the popularization that utilizes technology of capsicum heterosis, hybrid vigor is had great importance.
The production of hybrid seeds district that above-mentioned pepper plant can be survived the winter is: winter minimum temperature 〉=15 ℃, and the illumination abundance, the water source is the area easily.In the area that meets above-mentioned condition, pepper plant can both be survived the winter smoothly, and can not freeze to death, and can regerminate in 1 year again, grows new plant.
Embodiment
The invention will be further described with embodiment below.
Embodiment 1
Utilize the dried dual-purpose 2003A of being of green pepper type chilli kernel male sterile (commercial) to produce the method for capsicum first-filial generation seed, may further comprise the steps
1, carried out the capsicum cross experiment at Yunnan Province's Yuanmou County in 1 year, the this area capsicum can overwintering cultivation, winter minimum temperature 〉=15 ℃, the illumination abundance, the water source facility, sowing male parent respectively is that commercial recovery is 9104, female parent is commercial genic male sterility dual purpose 2003A, the all independent contiguous plant of the ratio of male parent and female parent 1: 8, male parent and female parent.The flower bud phase to initial bloom stage, pull out among the genic male sterility dual purpose 2003A about 50% educated strain, removing simultaneously that male parent recovers is assorted strain in 9104, hybridizes by conventional artificial pollination mode of the prior art, promptly makes the capsicum first-filial generation seed for commerial growing; Wherein:
Distance between capsicum hybrid seeding field and the capsicum production field is that 100 meters, middle plantation long-stalked crop corn carry out distance isolation; Planting of maternal density adopts the narrow bedding cultivation of zanjon, and furrow are wide 0.9 meter, a ridge cultivation 2 strains, and seeding row spacing 35 * 40cm, mu is planted 3000 strains; The density of male parent plantation is big slightly, is 4000 strains; After father and mother are originally bloomed, remove the ground floor flower, the pollen that utilizes male parent carries out artificial pollination to the flower of the maternal second layer and above opening, pollination time is 8:00 ~ 11:00 in morning every day, afternoon, 3:30 ~ 6:00 carried out the conventional cultivation management, the allocarpy on the results female parent, promptly obtain capsicum first-filial generation seed, use for commerial growing;
2, after the hybridization capsicum that harvests on the female parent, this plant of father and mother is not all cooked pruning, lets alone growth, when growing into November 15, pepper plant is pruned, and overground part fractionation 20cm height above the plant of cutting covered, watered once in 20 days;
3, after next year sprouts the plant sleeping bud after surviving the winter, prune and put in order, concrete grammar is: wait pepper plant to sprout that sprouting is long when high, to stay 2 growths vigorous to 10cm, and well-developed sprouting bud, other sproutings that sprouted are all removed; After 40 days, select the lateral bud of 1 robust growth, remove other lateral bud, carry out the conventional cultivation management;
4, repeating the step 3 time in 2,3 liang of steps, also is third and fourth, the five average annual steps that repeat 2,3 liang of steps; Promptly 1 year (once) sowing, recycling can continue to produce capsicum first-filial generation seed in 5 years.
Embodiment 2
The method of utilizing the dual-purpose 0908A of being of line green pepper type chilli kernel male sterile to produce capsicum first-filial generation seed
This example is substantially the same manner as Example 1, different only is: used chilli kernel male sterile is dual-purpose to be commercial line green pepper type, and the planting density of the dual-purpose system of chilli kernel male sterile is littler than dried green pepper type, mu is planted 2500 strains, used recovery is commercial 2001, isolate and adopt gauze to isolate, plant is trimmed to December 15, prune the back watered once in per 30 days, sprout Deng pepper plant that sprouting is long, to be stayed 2 to sprout buds, and only repeat 2 when high to 15cm, the step 1 in 3 liang of steps time, the time limit that also promptly continues to produce the hybridization pepper seed is 3 years altogether.
Claims (4)
1. one kind is the method for carrying out hybrid seeding with chilli kernel male sterile system and recovery, it is characterized in that process the following step:
A, production of hybrid seeds area or the booth that can survive the winter at pepper plant in 1 year, plant the dual-purpose system of chilli kernel male sterile respectively and recover system, florescence is removed the educated strain in the genic male sterility dual purpose, female parent is done in sterile strain with genic male sterility dual purpose, recover system and do male parent, carry out hybrid seeding, produce capsicum first-filial generation seed, be used for commerial growing;
B, sterile strain and the recovery that will gather in the crops in the genic male sterility dual purpose of hybrid seed are plant, carry out overwintering cultivation after the pruning of acrial part in the winter time;
C, next year prune and put in order after the plant sleeping bud after surviving the winter is sprouted, and do female parent at the florescence with male sterile line subsequently, and recovering is to do male parent to hybridize, and produces hybrid seed, is used for the capsicum commerial growing;
D, repetition B, C two steps 3 ~ 4 times.
2. according to the producing method for seed of claim 1, it is characterized in that, the production of hybrid seeds area that said pepper plant can be survived the winter is meant winter minimum temperature 〉=15 ℃, illumination abundance, water source be the area easily, in the area that meets above-mentioned condition, pepper plant can both be survived the winter smoothly, and can not freeze to death, can regerminate in next year, grow new plant.
3. according to the producing method for seed of claim 1, it is characterized in that said genic male sterility dual purpose is an abortion genic male sterility dual purpose completely; Contain the educated strain about 50% in the genic male sterility dual purpose of A plantation in 1 year in the step, can educate strain at the florescence and all pull out, and do female parent with remaining male sterile plant again, recovering is to do male parent to carry out hybrid seeding, production first-filial generation pepper seed is used for commerial growing.
4. according to the producing method for seed of claim 1, it is characterized in that, sterile strain and recovery that said B step will be gathered in the crops the genic male sterility dual purpose of hybrid seed are plant, carry out overwintering cultivation after the pruning of acrial part in the winter time, specific practice is: mid-November to early December, pepper plant is pruned overground part fractionation 20 ~ 30cm height, above the plant of cutting covered, 20 ~ 30d watered once; After next year sprouts the plant sleeping bud after surviving the winter in the said C step, prune and put in order, its concrete grammar is: it is long to 10 ~ 15cm when high to wait pepper plant to sprout sprouting, stay 1 ~ 2 growth vigorous, well-developed sprouting bud, other sproutings that sprouted are all removed, after 30 ~ 45 days, select the lateral bud of 1 robust growth, remove other lateral bud, carry out the conventional cultivation management.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910163216A CN101707977A (en) | 2009-12-23 | 2009-12-23 | Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910163216A CN101707977A (en) | 2009-12-23 | 2009-12-23 | Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line |
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| CN101707977A true CN101707977A (en) | 2010-05-19 |
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| CN200910163216A Pending CN101707977A (en) | 2009-12-23 | 2009-12-23 | Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870667A (en) * | 2012-10-12 | 2013-01-16 | 宝鸡市农业技术推广服务中心 | Open type cross breeding method of hot pepper cytoplasmic male sterile line |
| CN105766623A (en) * | 2016-04-07 | 2016-07-20 | 青岛金妈妈农业科技有限公司 | Production method for pepper hybrids |
-
2009
- 2009-12-23 CN CN200910163216A patent/CN101707977A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870667A (en) * | 2012-10-12 | 2013-01-16 | 宝鸡市农业技术推广服务中心 | Open type cross breeding method of hot pepper cytoplasmic male sterile line |
| CN105766623A (en) * | 2016-04-07 | 2016-07-20 | 青岛金妈妈农业科技有限公司 | Production method for pepper hybrids |
| CN105766623B (en) * | 2016-04-07 | 2018-05-11 | 青岛金妈妈农业科技有限公司 | A kind of producing method for seed of capsicum cenospecies |
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Open date: 20100519 |