CN103329795A - General method for rapidly obtaining novel cytoplasm male sterility restoration source of rice - Google Patents
General method for rapidly obtaining novel cytoplasm male sterility restoration source of rice Download PDFInfo
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- CN103329795A CN103329795A CN2013102898043A CN201310289804A CN103329795A CN 103329795 A CN103329795 A CN 103329795A CN 2013102898043 A CN2013102898043 A CN 2013102898043A CN 201310289804 A CN201310289804 A CN 201310289804A CN 103329795 A CN103329795 A CN 103329795A
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Abstract
The invention discloses a general method for rapidly obtaining a novel cytoplasm male sterility restoration source of rice. The method comprises the following steps: detecting the fertility of an original cytoplasm source donor according to the original cytoplasm source donor of the obtained cytoplasm male sterility material, and hybridizing the cytoplasm source donor material as a cytoplasm male sterility restoration source with the cytoplasm male sterility if the cytoplasm source donor material is normally male-fertile; and backing to the generation of the original donor material of the cytoplasm male sterility material if the cytoplasm male sterility material is male-sterile, and hybridizing the original donor material as the cytoplasm male sterility restoration source with the cytoplasm male sterility. The method allows the novel cytoplasm male sterility restoration source of rice to be rapidly obtained, is especially suitable for the like rice novel cytoplasm male sterility lines which allow good restoration sources to be not screened through a wide testcross process, and has a good repeatability and a reliable result.
Description
Technical field
The invention belongs to the technique for breeding of hybridized rice field, recover the universal method in source in particular to a kind of quick acquisition paddy rice cytoplasmic male sterilty.
Background technology
Since the seventies in 20th century, by paddy rice interspecific hybridization and the seed selection of nucleus substitution method paddy rice kytoplasm male (CMS) male sterile line of multiple separate sources.At present, the separate sources paddy rice cytoplasm male sterility line of using in the production,, protect relation extensive according to it totally can be divided into wild abortion type (WA-CMS), bag bench-type (BT-CMS) and red lotus type 3 kinds of main male sterile cytoplasm types such as (HL-CMS).
The recovery system that constantly cultivates the cytoplasm male sterility line with novel male sterile cytoplasm source and recover gene pool, be to avoid hybrid rice owing to long-term promote the effective technology means that single cell matter source produces potential risk, also be overcome current paddy rice cytoplasm male sterility line, recover be extensive because of being subjected in the seed selection process, protect relation specially the existing rice germplasm utilization of resources that brings of effect property restriction limit the effective way of this drawback mutually.
Behind the cytoplasm male sterility line of cultivating novel cell matter source, seek its corresponding cytoplasmic male sterilty and recover system, realize that " three are " (male sterile line, keep system and recover system) is supporting, be the key issue that breeder institute must solution.The most frequently used solution is that to utilize existing all kinds of recoveries be kind (the recovery system of WA-CMS, HL-CMS, BT-CMS) and to keep system and other rice varieties to carry out that extensive testcross and screening recovers be or germplasm that can be extensive that this method is commonly called as " extensively surveying extensive method ".Yet, in the process that the new germplasm source cytoplasm male sterility line is cultivated, usually run into by the extensive method of extensive survey and can not screen recovery system or recovery source.As, international perennial wild rice that paddy rice utilizes (female parent) is hybridized, is backcrossed with cultivated rice (hybridizing and the male parent of backcrossing), cultivation is from the novel cytoplasm male sterility line IR66707A in perennial wild rice cytoplasmic male sterilty matter source, China Jiaxing City Academy of Agricultural Sciences is sterile with this to be maternal, cultivate good 935A early, the male sterile line in this novel cytoplasmic male sterilty matter source does not find the good recovery source of restoring degree till now yet.
Summary of the invention
The invention provides the novel cytoplasmic male sterilty of a kind of quick acquisition paddy rice and recover the universal method in source.In order to realize purpose of the present invention, intend adopting following technical scheme:
One aspect of the present invention relates to the novel cytoplasmic male sterilty of quick acquisition paddy rice and recovers the universal method in source, it comprises the steps: the primary fine kytoplasm source donor according to the cytoplasmic male sterilty material that obtains, detect the fertility of this primary fine kytoplasm source donor, if this source of cytoplasm donor material is normal male-fertile, then hybridize as recovery source and this cytoplasmic male sterilty of this cytoplasmic male sterilty with the donor material of this source of cytoplasm; If this source of cytoplasm donor material is male sterile, then further date back to the original donor material that produces this cytoplasmic male sterilty material, hybridize as recovery source and this cytoplasmic male sterilty of this cytoplasmic male sterilty with this original donor material.
In a preferred embodiment of the present invention, described cytoplasm male sterility line is still to screen the novel cytoplasm male sterility line of paddy rice less than the good recovery source of restoring degree by " extensively surveying extensive method ".
In another preferred embodiment of the present invention, described cytoplasm male sterility line is that Dongxiang Wild Rice is to be kytoplasm, middle morning 35 cytoplasm male sterility line of cell nucleus background, and cytoplasmic male sterilty recovery source is Dongxiang Wild Rice.
By technical method provided by the present invention, can accelerate the supporting application of novel cytoplasmic male sterilty paddy rice " three are ", good reproducibility, reliable results.
Description of drawings:
Fig. 1: the novel cytoplasm male sterility line in Dongxiang Wild Rice kytoplasm source---middle 35A seed selection morning flow chart.
Embodiment
For maternal, indica type rice varieties morning " middle morning 35 " is for backcrossing male parent with Dongxiang Wild Rice (male normally can educate), backcrosses through 6 generations, and having obtained with the Dongxiang Wild Rice is to be kytoplasm, middle morning 35 the new germplasm source cytoplasm male sterility line (see figure 1) of cell nucleus background.
In order to determine guarantee the quality extensive guarantor's relation of male sterile line of this new germplasm source, we utilize the maintenance system of existing " wild abortion type ", " red lotus type " hybrid rice and recover system and conventional rice kind, respectively with this new germplasm source cytoplasm male sterility line in 35 test crosses early.All test cross F1 all are male sterile as a result, and sterile degree very thoroughly (table 1).Illustrate: the first, there is retentivity completely in the maintenance system of existing " wild abortion type ", " red lotus type " hybrid rice and recover system to the male sterility of this new germplasm source cytoplasm male sterility line; Second, extensive guarantor's relation of this new germplasm source cytoplasm male sterility line is different from " wild abortion type " and " red lotus type " fully, belong to novel cytoplasm male sterility line, because no matter be " wild abortion type " or " red lotus type ", it recovers system and keeps is all can not be to this novel sterile recovery capability that has; The 3rd, conventional variety (in the table 1 last 7) can not have recovery capability to this novel male sterile line, on the contrary, has hold facility completely.
Utilize the original donor material of the source of cytoplasm of this novel cytoplasm male sterility line---Dongxiang Wild Rice is male parent, carries out test cross with this novel male sterile line (female parent).The result shows, test cross F
1The pollen fertility of acting normally and ripening rate (table 2).Show that Dongxiang Wild Rice is the recovery source of this novel cytoplasm male sterility line.
Early 35A and test kind test cross F in the male sterile line in table 1 Dongxiang Wild Rice kytoplasm source
1But dyeing flower powder rate
1: WAR and WAB represent recovery system, the maintenance system of wild abortion type respectively in the bracket, and HL R and HL B represent recovery system, the maintenance system of red lotus type respectively.
Early 35A and Dongxiang Wild Rice test cross F in the male sterile line in table 2 Dongxiang Wild Rice kytoplasm source
1But dyeing flower powder rate and ripening rate
The above is the preferred embodiments of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (3)
1. obtain the universal method that the paddy rice cytoplasmic male sterilty recovers the source, it comprises the steps: the primary fine kytoplasm source donor according to the cytoplasmic male sterilty material that obtains, detect the fertility of this primary fine kytoplasm source donor, if this source of cytoplasm donor material is normal male-fertile, then hybridize as recovery source and this cytoplasmic male sterilty of this cytoplasmic male sterilty with the donor material of this source of cytoplasm; If this source of cytoplasm donor material is male sterile, then further date back to the original donor material that produces this cytoplasmic male sterilty material, hybridize as recovery source and this cytoplasmic male sterilty of this cytoplasmic male sterilty with this original donor material.
2. universal method according to claim 1, described cytoplasm male sterility line are still to screen the novel cytoplasm male sterility line of paddy rice less than the good recovery source of restoring degree by " extensively surveying extensive method ".
3. universal method according to claim 1, described cytoplasm male sterility line is that Dongxiang Wild Rice is to be kytoplasm, middle morning 35 cytoplasm male sterility line of cell nucleus background, cytoplasmic male sterilty recovery source is Dongxiang Wild Rice (male-fertile).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651100A (en) * | 2013-12-05 | 2014-03-26 | 江西省农业科学院水稻研究所 | Method for cultivating cytoplasmic male sterility restoring line |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1296736A (en) * | 2000-11-30 | 2001-05-30 | 武汉大学 | Method for breeding recovery line of trilinear super hybridized rice |
| JP2004283126A (en) * | 2003-03-25 | 2004-10-14 | Mitsui Chemicals Inc | Cytoplasmic male sterility restoring gene |
| CN1559189A (en) * | 2004-02-27 | 2005-01-05 | 华南农业大学 | Breeding method for rice of japonica type affined to indica rice restoring series |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1296736A (en) * | 2000-11-30 | 2001-05-30 | 武汉大学 | Method for breeding recovery line of trilinear super hybridized rice |
| JP2004283126A (en) * | 2003-03-25 | 2004-10-14 | Mitsui Chemicals Inc | Cytoplasmic male sterility restoring gene |
| CN1559189A (en) * | 2004-02-27 | 2005-01-05 | 华南农业大学 | Breeding method for rice of japonica type affined to indica rice restoring series |
Non-Patent Citations (3)
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| 张天真: "《作物育种学总论》", 30 March 2003 * |
| 牡丹江地区革命委员会科学技术局: "《农业科技手册》", 31 January 1973 * |
| 陈大洲等: "江西东乡野生稻细胞质雄性不育系的恢复源探讨", 《杂交水稻》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651100A (en) * | 2013-12-05 | 2014-03-26 | 江西省农业科学院水稻研究所 | Method for cultivating cytoplasmic male sterility restoring line |
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Application publication date: 20131002 |