CN103651100A - Method for cultivating cytoplasmic male sterility restoring line - Google Patents
Method for cultivating cytoplasmic male sterility restoring line Download PDFInfo
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- CN103651100A CN103651100A CN201310642753.8A CN201310642753A CN103651100A CN 103651100 A CN103651100 A CN 103651100A CN 201310642753 A CN201310642753 A CN 201310642753A CN 103651100 A CN103651100 A CN 103651100A
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Abstract
The invention discloses a method for cultivating a cytoplasmic male sterility restoring line. The method comprises the following steps: hybridizing a cytoplasmic sterility line and Dongxiang wild rice as a female parent and a male parent respectively to obtain F1; hybridizing the F1 as a female parent and a selected male parent to obtain recrossing F1; and backcrossing the recrossing F1 as a female parent and the selected male parent or another male parent through a backcrossing breeding process or a pedigree seed selection process to obtain the cytoplasmic male sterility restoring line. The invention aims to cultivate cytoplasmic male sterility restoring line from novel male sterility cytoplasm, and the restoring line thereof and cultivate and popularize novel cybridization rice, and effectively solves the latent risk generated in the long-term popularization of single cytoplasm sources, and the present mutual rice germplasm resource utilization restriction abuse caused by the restoring and maintaining relationship specialty restriction in the seed selection process of the present rice cytoplasm male sterility lines and restoring lines. The method is a general technologic method for culitivating cytoplasmic male sterility restoring lines, and has a good repeatability and a reliable result.
Description
Technical field
The invention belongs to technique for breeding of hybridized rice field, relate in particular to a kind of method of cultivating cytoplasmic male sterilty restorer.
Background technology
Since 20 century 70s, by paddy rice interspecific hybridization and the seed selection of nucleus substitution method paddy rice kytoplasm male (CMS) male sterile line of multiple separate sources.
At present, the separate sources paddy rice cytoplasm male sterility line of application in production,, guarantor relation extensive according to it totally can be divided into 3 kinds of main male sterile cytoplasm types such as wild abortion type (WA-CMS), bag bench-type (BT-CMS) and red lotus type (HL-CMS).
Cultivate one of hot fields of paying close attention to existing wild abortion type (WA-CMS), the cytoplasm male sterility line that wraps 3 kinds of novel fine kytoplasm sources that main male sterile cytoplasm type is extensive, guarantor's relation is different such as bench-type (BT-CMS) and red lotus type (HL-CMS) and the restorer person that is breeding of hybridized rice.
At present, how to avoid hybrid rice due to the long-term potential risk of promoting the generation of single cell matter source, how to solve drawback extensive because being subject in current paddy rice cytoplasm male sterility line, restorer Breeding Process, that the existing Rice Germplasm Resources utilization that specially restriction of effect property brings of guarantor's relation limits is mutually to perplex people's a great problem always.
Summary of the invention
The invention provides a kind of method of cultivating cytoplasmic male sterilty restorer, be intended to solve hybrid rice due to long-term promote extensive because of being subject in potential risk that single cell matter source produces and current paddy rice cytoplasm male sterility line, restorer Breeding Process, guarantor's relation specially effect property limit the problem that the existing Rice Germplasm Resources utilization that brings limits mutually.
The object of the present invention is to provide a kind of method of cultivating cytoplasmic male sterilty restorer, the method comprises the following steps:
Step 1, take " eastern wild type " cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridizes and obtains F
1;
Step 2, with F
1for female parent, select male parent, hybridize and obtain the F that reestablishes diplomatic relations
1;
Step 3, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, obtain " eastern wild type " cytoplasmic male sterilty restorer.
Further, in step 3, F also can reestablish diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, obtain " eastern wild type " cytoplasmic male sterilty restorer.
Further, the male parent of hybridizing, backcrossing require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
The male parent of further, reestablishing diplomatic relations require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
Further, in step 3, F reestablishes diplomatic relations
1for maternal requirement is: fringe neck degree of stretching out is good, panicle neck at least exposes the leaf sheath of sword-like leave; Pollen fertility is normal, and after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle.
Further, in step 3, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From 1 generation that backcrossed, select fringe neck degree of stretching out good, panicle neck at least exposes the leaf sheath of sword-like leave, pollen fertility is normal, after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle and the phenotype individual plant similar to the male parent that backcrosses and backcrosses with the male parent that backcrosses, and so continuous backcross is to 3-4 generation;
Selection fringe neck degree of stretching out is good, pollen fertility normal and the phenotype individual plant similar to the male parent that backcrosses, selfing 2-3 generation, from selfing 2-3 for strain, selection fringe neck degree of stretching out is good, pollen fertility is normally each strain that individual plant pollen fertility is normal, economical character is stable in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
Further, the F that reestablishes diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From the F that reestablishes diplomatic relations for the last time
1in generation, starts, the individual plant that select that fringe neck degree of stretching out is good, pollen fertility normal, meets breeding objective carries out selfing, selfing is stable to economical character so continuously, last therefrom select that fringe neck degree of stretching out is good, pollen fertility is normally normal, the strain that meets breeding objective of each individual plant pollen fertility in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
Further, meet breeding objective and refer to, the individual plant of selection should retain the advantage of the male parent of reestablishing diplomatic relations, and has improved the shortcoming of the male parent of reestablishing diplomatic relations, and flowering habit is good, pollen amount is large.
The method of cultivation cytoplasmic male sterilty restorer provided by the invention, take " eastern wild type " cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridizes and obtains F
1, with F
1for female parent, select male parent, hybridize and obtain the F that reestablishes diplomatic relations
1, with the F that reestablishes diplomatic relations
1for female parent, select same male parent to backcross, or select another one male parent to reestablish diplomatic relations, adopt backcross breeding or pedigree selection-breeding method, obtain " eastern wild type " cytoplasmic male sterilty restorer, the present invention is intended to cultivate cytoplasm male sterility line and the restorer thereof with Cytoplasmic male sterility source, cultivate and promote novel cybridization paddy rice, effectively solved hybrid rice due to the long-term potential risk of promoting the generation of single cell matter source, and current paddy rice cytoplasm male sterility line, extensive because being subject in restorer Breeding Process, the drawback that the existing Rice Germplasm Resources utilization that specially restriction of effect property brings of guarantor's relation limits mutually, the present invention is the current techique method of cultivating " eastern wild type " cytoplasmic male sterilty restorer, reproducible, reliable results, having stronger propagation and employment is worth.
Accompanying drawing explanation
Fig. 1 is the realization flow figure of the method for the cultivation cytoplasmic male sterilty restorer that provides of the embodiment of the present invention.
Fig. 2 is the specific implementation flow chart of the method for the cultivation cytoplasmic male sterilty restorer that provides of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, and be not used in restriction invention.
Fig. 1 shows the realization flow of the method for the cultivation cytoplasmic male sterilty restorer that the embodiment of the present invention provides.
The method comprises the following steps:
In step S101, take " eastern wild type " cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridize and obtain F
1;
In step S102, with F
1for female parent, select male parent, hybridize and obtain the F1 that reestablishes diplomatic relations;
In step S103, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, obtain " eastern wild type " cytoplasmic male sterilty restorer.
In embodiments of the present invention, in step S103, F also can reestablish diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, obtain " eastern wild type " cytoplasmic male sterilty restorer.
In embodiments of the present invention, the male parent of hybridizing, backcrossing require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
The male parent of reestablishing diplomatic relations in embodiments of the present invention, require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
In embodiments of the present invention, in step S103, F reestablishes diplomatic relations
1for maternal requirement is: fringe neck degree of stretching out is good, panicle neck at least exposes the leaf sheath of sword-like leave; Pollen fertility is normal, and after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle.
In embodiments of the present invention, in step S103, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From 1 generation that backcrossed, select fringe neck degree of stretching out good, panicle neck at least exposes the leaf sheath of sword-like leave, pollen fertility is normal, after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle and the phenotype individual plant similar to the male parent that backcrosses and backcrosses with the male parent that backcrosses, and so continuous backcross is to 3-4 generation;
Selection fringe neck degree of stretching out is good, pollen fertility normal and the phenotype individual plant similar to the male parent that backcrosses, selfing 2-3 generation, from selfing 2-3 for strain, selection fringe neck degree of stretching out is good, pollen fertility is normally each strain that individual plant pollen fertility is normal, economical character is stable in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
In embodiments of the present invention, the F that reestablishes diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From the F that reestablishes diplomatic relations for the last time
1in generation, starts, the individual plant that select that fringe neck degree of stretching out is good, pollen fertility normal, meets breeding objective carries out selfing, selfing is stable to economical character so continuously, last therefrom select that fringe neck degree of stretching out is good, pollen fertility is normally normal, the strain that meets breeding objective of each individual plant pollen fertility in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
In embodiments of the present invention, meet breeding objective and refer to, the individual plant of selection should retain the advantage of the male parent of reestablishing diplomatic relations, and has improved the shortcoming of the male parent of reestablishing diplomatic relations, and flowering habit is good, pollen amount is large.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 2, take " eastern wild type " cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridize and obtain F
1.Utilize this F
1for female parent, the kind good with Comprehensive Traits, pollen amount is large, coordinate force is good or product are male parent, hybridize and obtain the F that reestablishes diplomatic relations
1.Then, with these kinds or product, be that the male parent that backcrosses backcrosses, or repeatedly reestablish diplomatic relations.Backcross at every turn or reestablish diplomatic relations, all should select that fringe neck degree of stretching out is good, the normal individual plant of pollen fertility is that female parent backcrosses or reestablishes diplomatic relations.
If adopt back cross breeding method, from 1 generation that backcrossed, select fringe neck degree of stretching out good, the individual plant that pollen fertility is normal and phenotype is similar to the male parent that backcrosses backcrosses with the male parent that backcrosses, so continuous backcross is to 3-4 generation, then, therefrom select fringe neck degree of stretching out good, normal and the phenotype individual plant similar to the male parent that backcrosses of pollen fertility, selfing 2-3 generation, selfing 2-3 is in strain, select fringe neck degree of stretching out good, pollen fertility normal (in strain, each individual plant pollen fertility is normal) the stable strain of economical character (substantially), can become " eastern wild type " cytoplasmic male sterilty restorer.
If employing pedigree method, from the F that reestablishes diplomatic relations for the last time
1in generation, starts, selection fringe neck degree of stretching out is good, pollen fertility normal, (meeting breeding objective refers to meet breeding objective, middle menu strain has retained the advantage of the male parent of reestablishing diplomatic relations, improved the shortcoming of the male parent of reestablishing diplomatic relations, and flowering habit is good, pollen amount is large etc.) individual plant carry out selfing, so continuously selfing is basicly stable to economical character.Finally therefrom select that fringe neck degree of stretching out is good, pollen fertility normal (in strain, each individual plant pollen fertility is normal), meet the strain of breeding objective, can become " eastern wild type " cytoplasmic male sterilty restorer.The novel cytoplasmic male sterilty restorer of this class is referred to as " eastern wild type " cytoplasmic male sterilty restorer.
The method of the cultivation cytoplasmic male sterilty restorer that the embodiment of the present invention provides, take " eastern wild type " cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridizes and obtains F
1, with F
1for female parent, select male parent, hybridize and obtain the F that reestablishes diplomatic relations
1, with the F that reestablishes diplomatic relations
1for female parent, select same male parent to backcross, or select another one male parent to reestablish diplomatic relations, adopt backcross breeding or pedigree selection-breeding method, obtain " eastern wild type " cytoplasmic male sterilty restorer, the present invention is intended to cultivate cytoplasm male sterility line and the restorer thereof with Cytoplasmic male sterility source, cultivate and promote novel cybridization paddy rice, effectively solved hybrid rice due to the long-term potential risk of promoting the generation of single cell matter source, and current paddy rice cytoplasm male sterility line, extensive because being subject in restorer Breeding Process, the drawback that the existing Rice Germplasm Resources utilization that specially restriction of effect property brings of guarantor's relation limits mutually, the present invention is the current techique method of cultivating " eastern wild type " cytoplasmic male sterilty restorer, reproducible, reliable results, having stronger propagation and employment is worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. a method of cultivating cytoplasmic male sterilty restorer, is characterized in that, the method comprises the following steps:
Step 1, take cytoplasm male sterility line as maternal, take Dongxiang Wild Rice as male parent, hybridizes and obtains F
1;
Step 2, with F
1for female parent, select male parent, hybridize and obtain the F that reestablishes diplomatic relations
1;
Step 3, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, obtain " eastern wild type " cytoplasmic male sterilty restorer.
2. the method for claim 1, is characterized in that, in step 3, F also can reestablish diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, obtain " eastern wild type " cytoplasmic male sterilty restorer.
3. the method for claim 1, is characterized in that, the male parent of hybridizing, backcrossing require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
4. method as claimed in claim 2, is characterized in that, the male parent of reestablishing diplomatic relations require that Comprehensive Traits is good, pollen amount is large, coordinate force is good.
5. the method for claim 1, is characterized in that, in step 3, F reestablishes diplomatic relations
1for maternal requirement is: fringe neck degree of stretching out is good, panicle neck at least exposes the leaf sheath of sword-like leave; Pollen fertility is normal, and after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle.
6. the method for claim 1, is characterized in that, in step 3, with the F that reestablishes diplomatic relations
1for female parent, select the male parent of step 2 to backcross, adopt backcross breeding, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From 1 generation that backcrossed, select fringe neck degree of stretching out good, panicle neck at least exposes the leaf sheath of sword-like leave, pollen fertility is normal, after pollen I-KI dyeing, more than 90% pollen staining is dark blue, is shaped as circle and the phenotype individual plant similar to the male parent that backcrosses and backcrosses with the male parent that backcrosses, and so continuous backcross is to 3-4 generation;
Selection fringe neck degree of stretching out is good, pollen fertility normal and the phenotype individual plant similar to the male parent that backcrosses, selfing 2-3 generation, from selfing 2-3 for strain, selection fringe neck degree of stretching out is good, pollen fertility is normally each strain that individual plant pollen fertility is normal, economical character is stable in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
7. method as claimed in claim 2, is characterized in that, F reestablishes diplomatic relations
1for female parent, select another one male parent to reestablish diplomatic relations, adopt pedigree method, the concrete methods of realizing that obtains " eastern wild type " cytoplasmic male sterilty restorer is:
From the F that reestablishes diplomatic relations for the last time
1in generation, starts, the individual plant that select that fringe neck degree of stretching out is good, pollen fertility normal, meets breeding objective carries out selfing, selfing is stable to economical character so continuously, last therefrom select that fringe neck degree of stretching out is good, pollen fertility is normally normal, the strain that meets breeding objective of each individual plant pollen fertility in strain, can become " eastern wild type " cytoplasmic male sterilty restorer.
8. method as claimed in claim 7, is characterized in that, meets breeding objective and refers to, and the individual plant of selection should retain the advantage of the male parent of reestablishing diplomatic relations, and has improved the shortcoming of the male parent of reestablishing diplomatic relations, and flowering habit is good, pollen amount is large.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104067929A (en) * | 2014-06-17 | 2014-10-01 | 萍乡市农业科学研究所 | Method for establishing three-line multiple-grain restoring line breeding and multiple-grain hybrid rice thereof |
| CN105532432A (en) * | 2016-01-04 | 2016-05-04 | 安徽省农业科学院水稻研究所 | Rice restorer creation method applicable to mechanized seed production |
| CN107182778A (en) * | 2017-07-21 | 2017-09-22 | 江西省农业科学院水稻研究所 | A kind of screening technique of the affine maintainer of " eastern wild type " series of three-series hybrid rice outcrossing |
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| US20120246756A1 (en) * | 2011-03-18 | 2012-09-27 | Honda Motor Co., Ltd. | Method for producing rice f1 seed, rice f1 seed, and rice male sterile line |
| CN103329796A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source |
| CN103329795A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly obtaining novel cytoplasm male sterility restoration source of rice |
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| US20120246756A1 (en) * | 2011-03-18 | 2012-09-27 | Honda Motor Co., Ltd. | Method for producing rice f1 seed, rice f1 seed, and rice male sterile line |
| CN103329796A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067929A (en) * | 2014-06-17 | 2014-10-01 | 萍乡市农业科学研究所 | Method for establishing three-line multiple-grain restoring line breeding and multiple-grain hybrid rice thereof |
| CN104067929B (en) * | 2014-06-17 | 2016-08-24 | 萍乡市农业科学研究所 | A kind of three is composite grain rice Breeding for restoration lines and the method for creating of composite grain hybrid paddy rice thereof |
| CN105532432A (en) * | 2016-01-04 | 2016-05-04 | 安徽省农业科学院水稻研究所 | Rice restorer creation method applicable to mechanized seed production |
| CN107182778A (en) * | 2017-07-21 | 2017-09-22 | 江西省农业科学院水稻研究所 | A kind of screening technique of the affine maintainer of " eastern wild type " series of three-series hybrid rice outcrossing |
| CN107182778B (en) * | 2017-07-21 | 2019-04-09 | 江西省农业科学院水稻研究所 | A kind of affine screening technique for keeping system of " eastern wild type " series of three-series hybrid rice outcrossing |
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