CN109042293A - A kind of selection for Southeast Asia hybrid rice two-line sterile line - Google Patents

A kind of selection for Southeast Asia hybrid rice two-line sterile line Download PDF

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Publication number
CN109042293A
CN109042293A CN201810898284.9A CN201810898284A CN109042293A CN 109042293 A CN109042293 A CN 109042293A CN 201810898284 A CN201810898284 A CN 201810898284A CN 109042293 A CN109042293 A CN 109042293A
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rice
genotype
2abc
rice varieties
seed
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黄蓉
黎方仁
刘迎霞
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ANHUI HUARUN BIOTECHNOLOGY Co Ltd
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ANHUI HUARUN BIOTECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of selections for Southeast Asia hybrid rice two-line sterile line, the following steps are included: selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, make hybridization A/C and B/C, obtain first familiar generation, it is denoted as F1AC and F1BC, wherein, the genotype of high-temperature resistant water rice varieties A is aa, the genotype of waterlogging rice varieties B is bb, the genotype of high resistant to rice blast rice varieties C is cc, the genotype of F1AC is ac and the genotype of F1BC is bc, the present invention is used high-temperature resistant water rice varieties A, after waterlogging rice varieties B and high resistant to rice blast rice varieties C is hybridized and is returned, hybridize again with low-temperature-sensitive two-line sterile line, select two kinds of two-line sterile line strains, that is high temperature resistant disease resistance strain and resistance to Flooded disease resistance strain can be adapted for planting under the conditions of the Different climate of Southeast Asia different regions, applied widely.

Description

A kind of selection for Southeast Asia hybrid rice two-line sterile line
Technical field
It is specially a kind of for Southeast Asia hybrid rice two-line sterile line the present invention relates to rice breeding technology field Selection.
Background technique
Rice Cropping is concentrated mainly on Asia.Mainly from Chinese import hybrid rice seed, India carries out rice research for Vietnam Start to walk simultaneously with China, other countries also pay attention to scientific research, introduction and the popularization of hybrid rice to varying degrees, but it is horizontal with Still there is gap in China, and effect is not high.China is advanced in the world in terms of production technology of hybridized rice seed, but due to its biology Characteristic, applicability is not wide, and export trade scale Slow lifting does not show China's technical advantage.Therefore according to different zones ring Border foundation improves hybrid rice and educates, is numerous, pushing away the operational modes such as integration and mechanism, is future development important trend.
It is suitble to the Fine Quality Hybrid Rice new varieties of southeast asian market with enterprises as principal components breeding, promotes agriculture element orderly Flowing merges various countries' development innovation, growth linkage, interests along realization and has important meaning, and the weather master of south east asia It to be tropical rainy climate and tropical monsson climate, the former annual high temperature and rainy, the latter's high temperature and drought throughout the year, it would therefore be highly desirable to grind The resistance new rice variety for being suitble to south east asia growth is issued, for this purpose, proposing a kind of for two system of Southeast Asia hybrid rice Sterile line breeding method.
Summary of the invention
The purpose of the present invention is to provide a kind of selections for Southeast Asia hybrid rice two-line sterile line, with solution Certainly the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme: it is a kind of for two system of Southeast Asia hybrid rice infertility The selection of system, comprising the following steps:
1) selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, is made Hybridize A/C and B/C, obtain first familiar generation, be denoted as F1AC and F1BC, wherein the genotype of high-temperature resistant water rice varieties A is aa, waterlogging The genotype of rice varieties B is bb, and the genotype of high resistant to rice blast rice varieties C is cc, the genotype of F1AC be ac and The genotype of F1BC is bc；
2) F1AC is hybridized with F1BC, must reestablish diplomatic relations F1 generation, be denoted as F1ABC, genotype abcc；
3) in the seed of field diseases area plantation F1ABC, group selects without morbidity, disease resistance is good at 2500-3000 plants Plant, and harvest corresponding seed F2ABC, 50 parts, genotype abcc；
4) 25 parts of F2ABC and high-temperature resistant water rice varieties A are returned, obtain AF2ABC, then by remaining 25 parts of F2ABC It is returned with waterlogging rice varieties B, obtains BF2ABC, the genotype of AF2ABC is aacc, selects the genotype of BF2ABC to be bbcc；
5) seed of AF2ABC is seeded in field high-temperature region, the seed of BF2ABC is seeded in field flood area, is selected resistance to High temperatures good and good disease resistance AF2ABC plant, and AF seed is harvested, at the same time, select resistance to overhead flooding injury good, disease resistance is good BF2ABC plant, and harvest BF seed；
6) be female parent with low-temperature-sensitive two-line sterile line, in step 5) AF2ABC and BF2ABC into for male parent, respectively into Row hybridization, obtains hybrid AS1 and BS1, plants the seed of AS1 and BS1 respectively, and fertile strain is selected to be selfed, and obtains AS12 And BS12；
7) after AS12 and BS12 is selfed respectively, after selecting AS12 selfing, genotype aacc, two had excellent performance It is sterile line single plant one, after selecting BS12 selfing, genotype bbcc, the two-line sterile line single plant two haveing excellent performance.
Preferably, the high-temperature resistant water rice varieties A is military fortune round-grained rice 21 or military fortune round-grained rice 3.
Preferably, the waterlogging rice varieties B is black excellent No.1.
Preferably, the high resistant to rice blast rice varieties C is revive oily No. 1 famous-brand and high-quality seed rice Tianyou998, distant round-grained rice 92 or vibration Excellent 998.
Preferably, the field high-temperature region carries out localized hyperthermia's irradiation rice using incandescent lamp, and temperature is 38-45 DEG C.
Preferably, field flood area uses field part water irrigating depth for 10-15cm.
Compared with prior art, the beneficial effects of the present invention are:
The present invention, which uses, carries out high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C After hybridization and backcrossing, then hybridize with low-temperature-sensitive two-line sterile line, selects two kinds of two-line sterile line strains, i.e. high temperature resistant is disease-resistant Evil strain and waterlogging disease resistance strain, can be adapted for planting under the conditions of the Different climate of Southeast Asia different regions, Application Range Extensively, and using two groups of two-line sterile lines the rice grain planted out is full, and yield is high, while having good resistance.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described reality Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field Those of ordinary skill's every other embodiment obtained without making creative work, belongs to guarantor of the present invention The range of shield.
Embodiment 1
1) selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, is made Hybridize A/C and B/C, obtains first familiar generation, be denoted as F1ACAnd F1BC, wherein the genotype of high-temperature resistant water rice varieties A is aa, resistance to excess water The genotype of rice varieties B is bb, and the genotype of high resistant to rice blast rice varieties C is cc, F1ACGenotype be ac and F1BC's Genotype is bc, and high-temperature resistant water rice varieties A is military fortune round-grained rice 21, and waterlogging rice varieties B is black excellent No.1, high resistant to rice blast water Rice varieties C is distant round-grained rice 92；
2) by F1ACWith F1BCHybridized, must reestablish diplomatic relations F1 generation, be denoted as F1ABC, genotype abcc；
3) F is planted in field diseases area1ABCSeed, group is at 2500 plants, and selection is without morbidity, the good plant of disease resistance, And harvest corresponding seed F2ABC, 50 parts, genotype abcc；
4) by 25 parts of F2ABCIt is returned with high-temperature resistant water rice varieties A, obtains AF2ABC, then by remaining 25 parts of F2ABC and resistance to Excess water rice varieties B is returned, and BF is obtained2ABC, AF2ABCGenotype be aacc, select BF2ABCGenotype be bbcc；
5) by AF2ABCSeed be seeded in field high-temperature region, field high-temperature region carries out localized hyperthermia's irradiation using incandescent lamp Rice, temperature is 38 DEG C, by BF2ABCSeed be seeded in field flood area, field flood area use field part water irrigating depth for 10cm selects the AF that heat-resisting quantity is good and disease resistance is good2ABCPlant, and AF seed is harvested, at the same time, select resistance to overhead flooding injury It is good, the good BF of disease resistance2ABCPlant, and harvest BF seed；
It 6) is female parent with low-temperature-sensitive two-line sterile line, with the AF in step 5)2ABCAnd BF2ABCInto for male parent, carry out respectively Hybridization, obtains hybrid AS1 and BS1, plants the seed of AS1 and BS1 respectively, and fertile strain is selected to be selfed, obtain AS12 and BS12；
7) after AS12 and BS12 is selfed respectively, after selecting AS12 selfing, genotype aacc, two had excellent performance It is sterile line single plant one, after selecting BS12 selfing, genotype bbcc, the two-line sterile line single plant two haveing excellent performance.
Embodiment 2
1) selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, is made Hybridize A/C and B/C, obtains first familiar generation, be denoted as F1ACAnd F1BC, wherein the genotype of high-temperature resistant water rice varieties A is aa, resistance to excess water The genotype of rice varieties B is bb, and the genotype of high resistant to rice blast rice varieties C is cc, F1ACGenotype be ac and F1BC's Genotype is bc, and high-temperature resistant water rice varieties A is military fortune round-grained rice 21, and waterlogging rice varieties B is black excellent No.1, high resistant to rice blast water Rice varieties C is distant round-grained rice 92；
2) by F1ACWith F1BCHybridized, must reestablish diplomatic relations F1 generation, be denoted as F1ABC, genotype abcc；
3) F is planted in field diseases area1ABCSeed, group is at 2800 plants, and selection is without morbidity, the good plant of disease resistance, And harvest corresponding seed F2ABC, 50 parts, genotype abcc；
4) by 25 parts of F2ABCIt is returned with high-temperature resistant water rice varieties A, obtains AF2ABC, then by remaining 25 parts of F2ABC and resistance to Excess water rice varieties B is returned, and BF is obtained2ABC, AF2ABCGenotype be aacc, select BF2ABCGenotype be bbcc；
5) by AF2ABCSeed be seeded in field high-temperature region, field high-temperature region carries out localized hyperthermia's irradiation using incandescent lamp Rice, temperature is 42 DEG C, by BF2ABCSeed be seeded in field flood area, field flood area use field part water irrigating depth for 12cm selects the AF that heat-resisting quantity is good and disease resistance is good2ABCPlant, and AF seed is harvested, at the same time, select resistance to overhead flooding injury It is good, the good BF of disease resistance2ABCPlant, and harvest BF seed；
It 6) is female parent with low-temperature-sensitive two-line sterile line, with the AF in step 5)2ABCAnd BF2ABCInto for male parent, carry out respectively Hybridization, obtains hybrid AS1 and BS1, plants the seed of AS1 and BS1 respectively, and fertile strain is selected to be selfed, obtain AS12 and BS12；
7) after AS12 and BS12 is selfed respectively, after selecting AS12 selfing, genotype aacc, two had excellent performance It is sterile line single plant one, after selecting BS12 selfing, genotype bbcc, the two-line sterile line single plant two haveing excellent performance.
Embodiment 3
1) selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, is made Hybridize A/C and B/C, obtains first familiar generation, be denoted as F1ACAnd F1BC, wherein the genotype of high-temperature resistant water rice varieties A is aa, resistance to excess water The genotype of rice varieties B is bb, and the genotype of high resistant to rice blast rice varieties C is cc, F1ACGenotype be ac and F1BC's Genotype is bc, and high-temperature resistant water rice varieties A is military fortune round-grained rice 21, and waterlogging rice varieties B is black excellent No.1, high resistant to rice blast water Rice varieties C is distant round-grained rice 92；
2) by F1ACWith F1BCHybridized, must reestablish diplomatic relations F1 generation, be denoted as F1ABC, genotype abcc；
3) F is planted in field diseases area1ABCSeed, group is at 3000 plants, and selection is without morbidity, the good plant of disease resistance, And harvest corresponding seed F2ABC, 50 parts, genotype abcc；
4) by 25 parts of F2ABCIt is returned with high-temperature resistant water rice varieties A, obtains AF2ABC, then by remaining 25 parts of F2ABC and resistance to Excess water rice varieties B is returned, and BF is obtained2ABC, AF2ABCGenotype be aacc, select BF2ABCGenotype be bbcc；
5) by AF2ABCSeed be seeded in field high-temperature region, field high-temperature region carries out localized hyperthermia's irradiation using incandescent lamp Rice, temperature is 45 DEG C, by BF2ABCSeed be seeded in field flood area, field flood area use field part water irrigating depth for 15cm selects the AF that heat-resisting quantity is good and disease resistance is good2ABCPlant, and AF seed is harvested, at the same time, select resistance to overhead flooding injury It is good, the good BF of disease resistance2ABCPlant, and harvest BF seed；
It 6) is female parent with low-temperature-sensitive two-line sterile line, with the AF in step 5)2ABCAnd BF2ABCInto for male parent, carry out respectively Hybridization, obtains hybrid AS1 and BS1, plants the seed of AS1 and BS1 respectively, and fertile strain is selected to be selfed, obtain AS12 and BS12；
7) after AS12 and BS12 is selfed respectively, after selecting AS12 selfing, genotype aacc, two had excellent performance It is sterile line single plant one, after selecting BS12 selfing, genotype bbcc, the two-line sterile line single plant two haveing excellent performance.
Table 1 is the performance parameter table using the embodiment 1-3 two-line sterile line rice cultivation selected, and result is as follows:
As it can be seen from table 1 the two kinds of two-line sterile line kinds selected using the present invention, the rice pathogenesis of plantation Rate is low, and setting percentage is high, is adapted to the Different climate environment of Southeast Asia different regions completely, and embodiment 3 is optimal case.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (6)

1. a kind of selection for Southeast Asia hybrid rice two-line sterile line, it is characterised in that: the following steps are included:
1) selecting high-temperature resistant water rice varieties A, waterlogging rice varieties B and high resistant to rice blast rice varieties C is parent, makees hybridization A/ C and B/C, obtains first familiar generation, is denoted as F1ACAnd F1BC, wherein the genotype of high-temperature resistant water rice varieties A is aa, waterlogging rice varieties B Genotype be bb, the genotype of high resistant to rice blast rice varieties C is cc, F1ACGenotype be ac and F1BCGenotype be bc；
2) by F1ACWith F1BCHybridized, must reestablish diplomatic relations F1 generation, be denoted as F1ABC, genotype abcc；
3) F is planted in field diseases area1ABCSeed, group is at 2500-3000 plants, and selection is without morbidity, the good plant of disease resistance, And harvest corresponding seed F2ABC, 50 parts, genotype abcc；
4) by 25 parts of F2ABCIt is returned with high-temperature resistant water rice varieties A, obtains AF2ABC, then by remaining 25 parts of F2ABC and resistance to excess water Rice varieties B is returned, and BF is obtained2ABC, AF2ABCGenotype be aacc, select BF2ABCGenotype be bbcc；
5) by AF2ABCSeed be seeded in field high-temperature region, by BF2ABCSeed be seeded in field flood area, select heat-resisting quantity good And the AF that disease resistance is good2ABCPlant, and AF seed is harvested, at the same time, select resistance to overhead flooding injury good, the good BF of disease resistance2ABCIt plants Strain, and harvest BF seed；
It 6) is female parent with low-temperature-sensitive two-line sterile line, with the AF in step 5)2ABCAnd BF2ABCInto for male parent, hybridized respectively, Hybrid AS1 and BS1 are obtained, plants the seed of AS1 and BS1 respectively, and fertile strain is selected to be selfed, obtains AS12 and BS12；
7) after AS12 and BS12 is selfed respectively, after selecting AS12 selfing, genotype aacc, the two systems infertility haveing excellent performance It is single plant one, after selecting BS12 selfing, genotype bbcc, the two-line sterile line single plant two haveing excellent performance.
2. a kind of selection for Southeast Asia hybrid rice two-line sterile line according to claim 1, feature exist In: the high-temperature resistant water rice varieties A is military fortune round-grained rice 21 or military fortune round-grained rice 3.
3. a kind of selection for Southeast Asia hybrid rice two-line sterile line according to claim 1, feature exist In: the waterlogging rice varieties B is black excellent No.1.
4. a kind of selection for Southeast Asia hybrid rice two-line sterile line according to claim 1, feature exist In: the high resistant to rice blast rice varieties C is revive oily No. 1 famous-brand and high-quality seed rice Tianyou998, distant round-grained rice 92 or vibration excellent 998.
5. a kind of selection for Southeast Asia hybrid rice two-line sterile line according to claim 1, feature exist In: the field high-temperature region carries out localized hyperthermia using incandescent lamp and irradiates rice, and temperature is 38-45 DEG C.
6. a kind of selection for Southeast Asia hybrid rice two-line sterile line according to claim 1, feature exist In: field flood area uses field part water irrigating depth for 10-15cm.
CN201810898284.9A 2018-08-08 2018-08-08 A kind of selection for Southeast Asia hybrid rice two-line sterile line Pending CN109042293A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884931B2 (en) * 2002-08-30 2005-04-26 Ricetec, Inc. Rice hybrid RH101
CN104839010A (en) * 2015-05-21 2015-08-19 福建金山都发展有限公司 Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884931B2 (en) * 2002-08-30 2005-04-26 Ricetec, Inc. Rice hybrid RH101
CN104839010A (en) * 2015-05-21 2015-08-19 福建金山都发展有限公司 Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line

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Application publication date: 20181221