CN102630557A - Method for breeding functional formula rice varieties - Google Patents

Method for breeding functional formula rice varieties Download PDF

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CN102630557A
CN102630557A CN201210145861XA CN201210145861A CN102630557A CN 102630557 A CN102630557 A CN 102630557A CN 201210145861X A CN201210145861X A CN 201210145861XA CN 201210145861 A CN201210145861 A CN 201210145861A CN 102630557 A CN102630557 A CN 102630557A
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rice
proterties
performance
grain
select
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CN102630557B (en
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章清杞
陈幼玉
李美德
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Guangzhou Jing Yi Home Co. Ltd.
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a method for breeding functional formula rice varieties, belonging to the technical field of special rice breeding. The method is characterized by comprising the following steps of selecting a rice variety with larger extending area and good quality in production as the recurrent parent, crossing the recurrent parent with black rice, purple rice, red rice, glutinous rice and giant embryo rice respectively, backcrossing the offsprings with the recurrent parent, breeding the near-isogenic lines which have agronomic traits similar to the agronomic traits of the recurrent parent and respectively have the traits of the black rice, purple rice, red rice, glutinous rice and giant embryo rice and mixing the seeds in proportion to prepare the seeds for production of the formula rice. The method has the following beneficial effects that after the formula rice varieties bred by the method are planted, the shapes of plants and leaves and the agronomic traits are neat and consistent; the rice obtained by grinding unhulled rice can simultaneously contain the five functional rice, namely the black rice, the purple rice, the red rice, the glutinous rice and the giant embryo rice; the functional formula rice can be produced by planting the seeds; the production method is simple and is high in efficiency and good in benefits; and the produced high nutrition functional formula rice integrates color, aroma, taste and nutrition curative effect and has good health care function.

Description

A kind of selection of functional form prescription rice variety
Technical field
The present invention is specifically related to a kind of selection of functional form prescription rice variety, belongs to the special rice breeding technical field.
Background technology
In recent years; The alimentary health-care function of rice more and more receives consumer's attention; Some special rices; Because of it is of high nutritive value, mouthfeel is good, and some contained functional components have unique health-care efficacy and extremely consumer's favor to human body like black rice, purple rice, red rice, glutinous rice, giant embryo rice etc.Black rice has enriching yin and nourishing kidney, strengthening spleen and warming liver, qi and activate blood circulation, and curative effect such as nourish the liver to improve visual acuity, often edible black rice helps preventing and treating diseases such as Light-headedness, anaemia, xeropulmonary cough, constipation, poor appetite.Contain multivitamins such as abundant protein, fat, lysine, vitamin b3, sulphur peace element, folic acid in the purple rice; And needed by human body trace elements such as iron, zinc, calcium, phosphorus; It is the good merchantable brand of dietotherapy; Have blood yiqi, strong kidney profit liver, receive effect, particularly pregnant and lying-in women and rehabilitation patient's edible health of palace enriching yin, have very good effect.It is cream-coloured pink for red rice; Contain different trace elements, have the rising plasma hdl cholesterol, improve the effect of antioxidant ability of organism; To improving the antioxidant levels in the body through the normal diet approach, control has significant values because of the chronic disease that state of oxidation rising causes.Glutinous rice also belongs to extraordinary rice type, and glutinous rice is nice and can be processed into numerous food product, by numerous people are liked.Giant embryo rice contains needed by human body trace such as abundant GABA, protein and iron, zinc, calcium, phosphorus, and the prevention senile dementia is had very great help.These functional rice products have vast market prospect and are worth with exploitation; The economic benefit of its exploitation is considerable; Functional special rice market purchasing price is higher 2 to 5 times than common rice, and popularizing planting is for the income that improves vast kind of rice peasant, for the trophic level that improves broad masses of the people; Improving health all has great importance, and helps formation and the industrial restructuring and the adjustment of the new industry of China's rice simultaneously.
At present, the functional special rice product in China's rice and converted products market thereof is also less, can not satisfy the requirement of the raising of development of market economy and living standards of the people.More existing dissimilar rice mix on the domestic and international market at present " prescription rice " is sold; Its preparation method is that dissimilar rice separate plantation, production, husk rice; Mechanical mixture forms in proportion again, and this kind method operation is quite loaded down with trivial details, and production cost is high.
Summary of the invention
The object of the present invention is to provide a kind of selection of functional form prescription rice variety.
Content of the present invention is a kind of selection of functional form formula rice kind, and this prescription rice variety is the mixed type kind, and the paddy of plantation grinds the rice that can contain black rice, purple rice, red rice, glutinous rice, 5 kinds of functional rices of giant embryo rice simultaneously.Its method is: through select for use in the present production the big and quality of popularizing area preferably rice varieties make recurrent parent; Respectively with black rice rice, purple rice rice, red rice rice, glutinous rice, giant embryo rice hybridization; In offspring and recurrent parent many generations, backcross, and seed selection economical character and recurrent parent are close, the proterties neat and consistent, stablizes and do not separate; And the NIL strain that has black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties respectively, the production that mixes prescription rice by a certain percentage is with planting.
Technical scheme of the present invention is following:
(1) recurrent parent R (good standing grain early accounts for, good spoke accounts for, FR315 or bright extensive 86); With black rice rice (Qin rice No. 2), purple rice rice (inferior bigger purple rice), red rice rice (Anhui rice 129), glutinous rice (glutinous No. 6 of chaste tree) and giant embryo rice (MhgeR) hybridization, must hybridize F respectively 1In generation, is (respectively with F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 expressions);
(2) plantation F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 seeds, flowering stage and recurrent parent R backcross to such an extent that backcrossed for 1 generation respectively, respectively with BC 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 expressions;
(3) results backcross 1 generation seed, the screening targets of shelling proterties seed, wherein BC 1F 1-1 selects the brown rice grain of performance black rice proterties, BC 1F 1-2 select the brown rice grain of performance purple rice proterties, BC 1F 1-3 select the brown rice grain of performance red rice proterties, BC 1F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 1F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(4) the 1 generation strain BC that backcrosses that obtains is screened in plantation 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 seeds are investigated each strain flowering stage and are selected economical character each 5 of individual plants close with recurrent parent R and that the loose powder proterties of blooming is good and continue to backcross to such an extent that backcrossed for 2 generations respectively with recurrent parent R, respectively with BC 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 expressions;
(5) results backcross 2 generation seed, the screening targets of shelling proterties seed, wherein BC 2F 1-1 selects the brown rice grain of performance black rice proterties, BC 2F 1-2 select the brown rice grain of performance purple rice proterties, BC 2F 1-3 select the brown rice grain of performance red rice proterties, BC 2F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 2F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(6) BC that obtains is screened in plantation 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 seeds are investigated each strain flowering stage and in each strain, are selected economical character and bloom loose powder proterties good individual plant each 5 close with recurrent parent R and recurrent parent R to continue to backcross to such an extent that backcrossed for 3 generations respectively, respectively with BC 3F 1-1, BC 3F 1-2, BC 3F 1-3, BC 3F 1-4, BC 3F 1-5 expressions;
(7) continue step (5), (6), up to backcrossing for 6 generations, each strain economical character of backcrossing does not separate with recurrent parent R is consistent basically, and have black rice respectively, purple rice, red rice, glutinous rice, giant embryo rice properties and characteristics; The economical character of breeding is consistent with recurrent parent R, and the NIL strain difference called after article 1, article 2, article 3, article 4, the article 5 that have black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties respectively;
(8) article 1, article 2, article 3, article 4, article 5 difference breeding seeds, breeding seed mixes promptly to get by 1:1:1:1:1 or 1:1:1:2:2 produces the seed of using the prescription rice varieties.
Beneficial effect of the present invention is to import the gene of control black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties in the same kind through the back cross breeding method respectively; Breed the NIL that contains identical genetic background; Economical characters such as the leaf attitude of these NIL strains are close; The rice strain that mixed planting is come out is neat and consistent also, its seed that mixes in proportion of plantation in the production, and the paddy husk rice of being gathered in the crops is the high trophic function type prescription rice of 5 type combination.Produce " prescription rice " seed through breeding method of the present invention, plant this seed and can produce functional form " prescription rice ".Production method is simple, efficient is high, profitable; Save operation and cost than traditional " prescription rice " production method; And the high trophic function type prescription rice of producing is rich in natural nutrition element and physiological activators such as GABA, anthocyan, carotin, cardiac glycoside, protein, several mineral materials, amino acid, vitamin; Normal food can improve health, and regulates body ' s physiological rhythm.Functional form " prescription rice " integrates natural color and nutrition curative effect, has great health care function.
Embodiment
Embodiment 1:
(1) selects good standing grain early to account for and make recurrent parent, with black rice rice-Qin rice No. 2, the bigger purple rice of purple rice rice-Ya, red rice rice-Anhui rice 129, glutinous rice-chaste tree glutinous No. 6 and giant embryo rice-MhgeR hybridization, must hybridize F respectively 1For F 1-1, F 1-2, F 1-3, F 1-4, F 1-5;
(2) plantation F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 seeds, flowering stage and good standing grain early account for respectively to be backcrossed, and 1 generation BC must backcross 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5;
(3) results backcross 1 generation seed, the screening targets of shelling proterties seed, wherein BC 1F 1-1 selects the brown rice grain of performance black rice proterties, BC 1F 1-2 select the brown rice grain of performance purple rice proterties, BC 1F 1-3 select the brown rice grain of performance red rice proterties, BC 1F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 1F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(4) BC that obtains is screened in plantation 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 seeds, investigate each strain flowering stage and select economical character and good standing grain early accounts for close and each 5 of individual plants that the loose powder proterties of blooming is good and good standing grain early account for continue respectively to backcross the 2 generation BC that backcross 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5;
(5) results backcross 2 generation seed, the screening targets of shelling proterties seed, wherein BC 2F 1-1 selects the brown rice grain of performance black rice proterties, BC 2F 1-2 select the brown rice grain of performance purple rice proterties, BC 2F 1-3 select the brown rice grain of performance red rice proterties, BC 2F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 2F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(6) BC that obtains is screened in plantation 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 seeds, investigate each strain flowering stage and in each strain, select economical character is close with recurrent parent R and the loose powder proterties of blooming is good each 5 of individual plants and recurrent parent R continue respectively to backcross the 3 generation BC that backcross 3F 1-1, BC 3F 1-2, BC 3F 1-3, BC 3F 1-4, BC 3F 1-5;
(7) continue step (5), (6),, breed economical character and good standing grain and early account for and consistently do not separate up to backcrossing for 6 generations, and have black rice respectively, purple rice, red rice, glutinous rice, giant embryo rice properties and characteristics;
(8) early account for consistent with good standing grain the economical character of breeding; And the NIL difference breeding seed that has black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties respectively, the seed of breeding mix promptly to get by 1:1:1:1:1 or 1:1:1:2:2 produces the seed of using the prescription rice varieties.The functional form " prescription rice " that this prescription rice varieties is produced contains GABA 6mg/100g, protein 9.4%, total amino acid 9.6%, Cobastab 14.6mg/kg, Cobastab 20.91mg/kg, also being rich in natural nutrition element and physiological activators such as anthocyan, carotin, cardiac glycoside and various mineral matters, normal food can improve health, and regulates body ' s physiological rhythm.
Embodiment 2:
(1) selects FR315 to make recurrent parent, with black rice rice-Qin rice No. 2, the bigger purple rice of purple rice rice-Ya, red rice rice-Anhui rice 129, glutinous rice-chaste tree glutinous No. 6 and giant embryo rice-MhgeR hybridization, must hybridize F respectively 1For F 1-1, F 1-2, F 1-3, F 1-4, F 1-5;
(2) plantation F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 seeds, flowering stage and FR315 backcross respectively, and 1 generation BC must backcross 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5;
(3) results backcross 1 generation seed, the screening targets of shelling proterties seed, wherein BC 1F 1-1 selects the brown rice grain of performance black rice proterties, BC 1F 1-2 select the brown rice grain of performance purple rice proterties, BC 1F 1-3 select the brown rice grain of performance red rice proterties, BC 1F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 1F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(4) BC that obtains is screened in plantation 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 seeds, investigate each strain flowering stage and select economical character and each 5 of FR315 is close and the loose powder proterties of blooming is good individual plants and FR315 continue respectively to backcross the 2 generation BC that backcross 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5;
(5) results backcross 2 generation seed, the screening targets of shelling proterties seed, wherein BC 2F 1-1 selects the brown rice grain of performance black rice proterties, BC 2F 1-2 select the brown rice grain of performance purple rice proterties, BC 2F 1-3 select the brown rice grain of performance red rice proterties, BC 2F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 2F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(6) BC that obtains is screened in plantation 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 seeds, investigate each strain flowering stage and in each strain, select economical character is close with recurrent parent R and the loose powder proterties of blooming is good each 5 of individual plants and recurrent parent R continue respectively to backcross the 3 generation BC that backcross 3F 1-1, BC 3F 1-2, BC 3F 1-3, BC 3F 1-4, BC 3F 1-5;
(7) continue step (5), (6),, breed that economical character is consistent with FR315 not to be separated up to backcrossing for 6 generations, and have black rice respectively, purple rice, red rice, glutinous rice, giant embryo rice properties and characteristics;
(8) economical character of breeding is consistent with FR315; And the NIL difference breeding seed that has black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties respectively, the seed of breeding mix promptly to get by 1:1:1:1:1 or 1:1:1:2:2 produces the seed of using the prescription rice varieties.The functional form " prescription rice " that this prescription rice varieties is produced contains GABA 6mg/100g, protein 9.4%, total amino acid 9.6%, Cobastab 14.6mg/kg, Cobastab 20.91mg/kg, also being rich in natural nutrition element and physiological activators such as anthocyan, carotin, cardiac glycoside and various mineral matters, normal food can improve health, and regulates body ' s physiological rhythm.
Embodiment 3:
(1) selects bright extensive 86 to make recurrent parent, with black rice rice-Qin rice No. 2, the bigger purple rice of purple rice rice-Ya, red rice rice-Anhui rice 129, glutinous rice-chaste tree glutinous No. 6 and giant embryo rice-MhgeR hybridization, must hybridize F respectively 1For F 1-1, F 1-2, F 1-3, F 1-4, F 1-5;
(2) plantation F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 seeds, backcross flowering stage and bright extensive 86 respectively, and 1 generation BC must backcross 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5;
(3) results backcross 1 generation seed, the screening targets of shelling proterties seed, wherein BC 1F 1-1 selects the brown rice grain of performance black rice proterties, BC 1F 1-2 select the brown rice grain of performance purple rice proterties, BC 1F 1-3 select the brown rice grain of performance red rice proterties, BC 1F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 1F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(4) BC that obtains is screened in plantation 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 seeds, investigate each strain flowering stage and select economical character and bright extensive 86 close and each 5 and bright extensive 86 of individual plants that the loose powder proterties of blooming is good continue respectively to backcross the 2 generation BC that backcross 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5;
(5) results backcross 2 generation seed, the screening targets of shelling proterties seed, wherein BC 2F 1-1 selects the brown rice grain of performance black rice proterties, BC 2F 1-2 select the brown rice grain of performance purple rice proterties, BC 2F 1-3 select the brown rice grain of performance red rice proterties, BC 2F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 2F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(6) BC that obtains is screened in plantation 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 seeds, investigate each strain flowering stage and in each strain, select economical character is close with recurrent parent R and the loose powder proterties of blooming is good each 5 of individual plants and recurrent parent R continue respectively to backcross the 3 generation BC that backcross 3F 1-1, BC 3F 1-2, BC 3F 1-3, BC 3F 1-4, BC 3F 1-5;
(7) continue step (5), (6),, breed that economical character is consistent with bright extensive 86 not to be separated up to backcrossing for 6 generations, and have black rice respectively, purple rice, red rice, glutinous rice, giant embryo rice properties and characteristics;
(8) economical character of breeding is consistent with bright extensive 86; And the NIL difference breeding seed that has black rice, purple rice, red rice, glutinous rice, giant embryo rice proterties respectively, the seed of breeding mix promptly to get by 1:1:1:1:1 or 1:1:1:2:2 produces the seed of using the prescription rice varieties.The functional form " prescription rice " that this prescription rice varieties is produced contains GABA 6mg/100g, protein 9.4%, total amino acid 9.6%, Cobastab 14.6mg/kg, Cobastab 20.91mg/kg, also being rich in natural nutrition element and physiological activators such as anthocyan, carotin, cardiac glycoside and various mineral matters, normal food can improve health, and regulates body ' s physiological rhythm.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.

Claims (1)

1. the selection of functional form prescription rice variety, it is characterized in that: the concrete steps of said selection are following:
(1) with good standing grain early account for, good spoke accounts for, FR315 or bright extensive 86 be that recurrent parent R is hybridized with black rice rice-Qin rice No. 2, the bigger purple rice of purple rice rice-Ya, red rice rice-Anhui rice 129, glutinous rice-chaste tree glutinous No. 6 and giant embryo rice-MhgeR respectively, must hybridize F 1In generation, is respectively with F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 expressions;
(2) plantation F 1-1, F 1-2, F 1-3, F 1-4, F 1-5 seeds, flowering stage and recurrent parent R backcross to such an extent that backcrossed for 1 generation respectively, respectively with BC 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 expressions;
(3) results backcross 1 generation seed, the screening targets of shelling proterties seed, wherein BC 1F 1-1 selects the brown rice grain of performance black rice proterties, BC 1F 1-2 select the brown rice grain of performance purple rice proterties, BC 1F 1-3 select the brown rice grain of performance red rice proterties, BC 1F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 1F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(4) BC that obtains is screened in plantation 1F 1-1, BC 1F 1-2, BC 1F 1-3, BC 1F 1-4, BC 1F 1-5 seeds are investigated each strain flowering stage and are selected economical character each 5 of individual plants close with recurrent parent R and that the loose powder proterties of blooming is good and continue to backcross to such an extent that backcrossed for 2 generations respectively with recurrent parent R, respectively with BC 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 expressions;
(5) results backcross 2 generation seed, the screening targets of shelling proterties seed, wherein BC 2F 1-1 selects the brown rice grain of performance black rice proterties, BC 2F 1-2 select the brown rice grain of performance purple rice proterties, BC 2F 1-3 select the brown rice grain of performance red rice proterties, BC 2F 1-4 select the brown rice grain of performance glutinous rice proterties, BC 2F 1-5 select the brown rice grain of performance giant embryo rice proterties;
(6) BC that obtains is screened in plantation 2F 1-1, BC 2F 1-2, BC 2F 1-3, BC 2F 1-4, BC 2F 1-5 seeds are investigated each strain flowering stage and in each strain, are selected each 5 of the individual plants that economical character is close with recurrent parent R and the loose powder proterties of blooming is good to continue to backcross to such an extent that backcrossed for 3 generations respectively with recurrent parent R, respectively with BC 3F 1-1, BC 3F 1-2, BC 3F 1-3, BC 3F 1-4, BC 3F 1-5 expressions;
(7) continue step (5), (6); Up to backcrossing for 6 generations; Breed economical character and do not separate with recurrent parent R is consistent, and have black rice respectively, the NIL of purple rice, red rice, glutinous rice, giant embryo rice properties and characteristics, 5 NILs are called after article 1, article 2, article 3, article 4, article 5 respectively;
(8) article 1, article 2, article 3, article 4, article 5 difference breeding seeds, breeding seed mixes promptly to get by 1:1:1:1:1 or 1:1:1:2:2 produces the seed of using the prescription rice variety.
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