CN106171941B - A kind of selection of highly resistance colour soybean varieties - Google Patents

A kind of selection of highly resistance colour soybean varieties Download PDF

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CN106171941B
CN106171941B CN201610527887.9A CN201610527887A CN106171941B CN 106171941 B CN106171941 B CN 106171941B CN 201610527887 A CN201610527887 A CN 201610527887A CN 106171941 B CN106171941 B CN 106171941B
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soybean
seed
character
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recurrent parent
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CN106171941A (en
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章清杞
陈幼玉
章珍
陈志伟
杨帆
李美德
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Fuzhou andI Agricultural Technology Development Co., Ltd.
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Genetics & Genomics (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of selections of highly resistance colour soybean varieties, belong to disease-resistant extraordinary soybean breeder technical field.The present invention selects yellow soybean varieties to make recurrent parent, hybridize respectively with the soybean varieties of black soybean, green soybean, brown soybean, highly resistance heart-eating worm, cyst nematode disease and grey speck of soybean, offspring is returned with recurrent parent mostly generation, hybridized two-by-two with Resistant strain by coloured strain again, screening through mostly generation kind skin and Resistant character, selection and breeding are respectively provided with the soybean near isogenic lines of Black seed coat, green kind of skin, brown seed coat color properties and characteristics, mix the production kind of formula highly resistance colour soybean in proportion with soya bean recurrent parent.It is high using this method selection and breeding Soybean Production, better resistance, its soybean harvested contains black soybean, green soybean, brown soybean, soybean, and the colored soybean of colour soybean varieties production integrates color and nutrition curative effect, has good alimentary health-care function.

Description

A kind of selection of highly resistance colour soybean varieties
Technical field
Present invention relates particularly to a kind of selections of highly resistance colour soybean varieties, belong to extraordinary soybean breeder technology neck Domain.
Background technology
Soybean is native to China, is a kind of legume that its seed contains abundant phytoprotein and fat, soybean The wider model of purposes, is usually used in squeezing soya-bean oil, makes various bean product and extraction protein etc., and the byproduct dregs of beans after oil expression is The most important protein raw material of feed industry.Grey speck of soybean, heart-eating worm, cyst nematode disease are the main diseases and insect pests of soybean, tool Have often hair property and it is sudden, seriously affect soybean yields and the raising of quality.Grey speck of soybean is normal hair venereal disease evil, is by soybean Tail spore bacterium fungal infection and fall ill.Have an impact to the yield and quality of soybean, the general plot underproduction 10-15%, harm is serious The plot underproduction up to 30% or more.Eating-core bean worm is the important pests in China soybean producing region, is distributed mainly on North of Yangtze River, Especially northern bean producing region.Eating-core bean worm feeding habits are single, mainly cause harm soybean and wild soybean.Soybean cyst nematode It is called verticillium wilt, it is worldwide soybean diseases, and there are large area hair in the U.S. of main Soybean production state, Brazil, China and Japan It is raw.China occurs mainly in the ground such as northeast, North China, Henan, Shandong and Anhui.Especially with Liaoning Province of three provinces in the northeast of China Kangping, Jilin Province The area such as Baicheng Prefecture, the Zhaodong of Heilongjiang Province, Anda, grand celebration, Qiqihar occurs serious.Generally make soybean underproduction 10%- 20%, serious underproduction 70%-90%, or even total crop failure.The prevention of soybean diseases, it is especially simultaneous to resist how anti-product to use disease-resistant variety Kind it is generally acknowledged in the world most economical effective measures.Soybean contains a large amount of protein, while also containing there are many needed by human Amino acid plays human tissue cell important trophism, can improve immune function of human body.In addition to containing high egg in soybean In vain, high in fat outer, also there is the nutritional ingredient of healthcare function containing there are many, such as containing adjustable human endocrine, raising is exempted from Epidemic disease power alleviates women's climacteric syndrome and anticancer, anti-oxidant, anti-hemolysis, the isoflavones for reducing the effects that cholesterol, Lecithin in soybean can remove the cholesterol being attached on vascular wall, prevent vascular sclerosis, prevention of cardiovascular disease from protecting the heart It is dirty.Contain several mineral materials in soybean, supplement calcium prevents the osteoporosis caused by calcium deficiency, promotes skeleton development.In soybean The Soluble Fiber contained, not only can defaecation, but also cholesterol can be reduced.Not only content is more for iron in soybean, but also easily by human body institute It absorbs, has certain curative effect to hypoferric anemia.Saponin contained by soybean has apparent effect for reducing blood fat, meanwhile, it can inhibit weight Increase, reduces lipid content and fat content in serum, liver.Adjustment with people's diet structure and soybean intensive processing industry Development, soybean are just gradually developed from the demand to protein and fat to the multiduty direction of diversification, and specificity and spy use Property increasingly highlights, and with the raising of health care consciousness, the healthcare function of extraordinary soybean is increasingly subject to the attention of people.Especially It is rich in the black soybean of the healthy trace elements with household such as selenium, iron, green soya bean is containing abundant protein, wherein containing a variety of ammonia needed by human Base acid even more has been favored by people especially with lysine content height.In recent years, the work(such as black soybean, green soybean, high-oil-content soybean Energy property soybean prod has a vast market foreground and Development volue, the economic benefits of exploitation, and extraordinary soybean market is received Purchase that price is higher than common soybeans, popularizing planting is for improving the incomes of vast farmers, for improving broad masses of the people Trophic level improves health all and has great importance.
Currently, China's functional special soybean prod is also less, it is mixed to only have some different type soybean on domestic and international market " formula soybean " sale altogether, production method are different type soybean difference plant development, then mechanical mixture in proportion It forms, such method process is quite cumbersome, and production cost is high, and the functional special soybean varieties of high disease and insect resistance are then more It is rare.
Invention content
Present disclosure is a kind of selection of highly resistance colour soybean varieties, and it is an object of the present invention to provide a kind of high Resistant The selection of harmful colored soybean varieties selects high yield and high quality soybean kind using genetic principle and cross breeding method Make recurrent parent, respectively with the soybean of black soybean, green soybean, brown soybean and highly resistance heart-eating worm, cyst nematode disease, grey speck of soybean Mixing breed, offspring is returned with recurrent parent mostly generation, then is hybridized two-by-two with Resistant strain by coloured strain, through mostly generation kind skin With the screening of Resistant character, selecting leaf morphology and economical character is close with recurrent parent, trait expression does not detach unanimously, Yield is high, better resistance and the soybean near isogenic lines for being respectively provided with Black seed coat, green kind of skin, brown seed coat color properties and characteristics, It is mixed in a certain ratio the production kind into formula highly resistance colour soybean with soya bean recurrent parent.Utilize the method for the present invention selection and breeding The plantation of highly resistance colour soybean varieties after leaf morphology and Agronomic neat and consistent, yield is high, better resistance, but it is received The soybean obtained contains black soybean, green soybean, brown soybean, soybean, the colour soybean varieties production colored soybean collection color, perfume (or spice), Taste and nutrition curative effect, have good alimentary health-care function.The colour soybean varieties are bulk variety, and highly resistance eats the heart Worm, cyst nematode disease, grey speck of soybean, as long as the primary bulk variety of plantation, can harvest simultaneously containing black soybean, green soybean, The soybean of 4 kinds of brown soybean, soybean colors, this method can not only produce the functional soy of high nutrition, moreover it is possible to simplify " formula The production process of soybean " reduces production cost, greatly improves farmers' income.
Technical scheme is as follows：
1) high yield and high quality highly resistance soybean varieties Ji agriculture 40 is selected（It is represented with parent R）Make recurrent parent, eats the heart with highly resistance respectively Worm soybean varieties Jilin 16（P1）, the high anti-line 1 of resistance to SCN soybean varieties（P2）, highly resistance grey speck of soybean kind close Rich 29（P3）, black soybean kind dragon black soybean 2 or southern agriculture 26（P4）, green soybean kind north agriculture 106 or Shanxi product 1（P5）, it is brown Soybean varieties are bright to educate 0305（P6）Hybridization, P1, P2, P3, P4, P5, P6 make male parent, hybridize to obtain hybridization F1In generation, is respectively with F1-A、F1- B、F1-C、F1-D、F1-E、F1- F is indicated；
2) plantation hybridization F1For seed F1-A、F1-B、F1-C、F1-D、F1-E、F1Each 5 plants of-F, florescence is respectively therefrom selected excellent Single plant 2 and recurrent parent R（Make maternal）It is returned to obtain 1 generation BC of backcrossing respectively1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、 BC1F1-E、BC1F1-F；
3) 1 generation BC of plantation backcrossing1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、BC1F1- F seeds, maturity period receive Obtain seed（BC1F2）, to BC1F1-A、BC1F1-B、BC1F1The soya seeds BC of-C harvests1F2Kernel seed coat colour is screened, wherein BC1F2The seed 20 of-A selection performance Black seed coat characters, BC1F2The seed 20 of green kind of the skin character of-B selection performances, BC1F2The seed 20 of-C selection performance brown seed coat color characters；
4) BC that plantation screening obtains1F2-A、BC1F2-B、BC1F2- C seeds and without screening to obtain BC1F2-D、BC1F2-E、 BC1F2- F seeds, to BC1F2-D、BC1F2-E、BC1F2The pest-resistant disease resistance of 3 combinations such as-F is identified that each combination is only reflected Its fixed corresponding resistant parent institute Resistant kind, screening highly resistance and agronomy and yield traits and recurrent parent R phases from each combination Close single plant 3 continues to be returned respectively with maternal recurrent parent is made, BC1F2-A、BC1F2-B、BC1F2- C strains also select agronomy It is close with recurrent parent R with yield traits and bloom each 3 of the good elite seedling of character with make maternal recurrent parent R respectively after It is continuous to be returned to obtain 2 generation BC of backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1-F；
5) 2 generation BC of plantation backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1- F seeds, maturity period receive Obtain seed（BC2F2）, to BC2F2-A、BC2F2-B、BC2F2The kernel seed coat colour of-C soya seeds is screened, wherein BC2F2- A is selected Select the seed 20 of performance black character, BC2F2The seed 20 of the green character of-B selection performances, BC2F2- C selection performance brown The seed of character 20；
6) BC that plantation screening obtains2F2Strain BC2F2-A、BC2F2-B、BC2F2- C seeds and without screening to obtain BC2F2-D、 BC2F2-E、BC2F2- F seeds, to BC2F2-D、BC2F2-E、BC2F2The pest-resistant disease resistances of the combination of-F etc. 3 are identified, each Its corresponding resistant parent institute Resistant kind is only identified in combination, screening highly resistance and agronomy and yield traits and samsara from each combination Single plant 3 and recurrent parent similar in parent R（Make maternal）Continue to be returned respectively, BC2F2-A、BC2F2-B、BC2F2- C strains Selection agronomy and yield traits are close with recurrent parent R and bloom each 3 of the good elite seedling of character and recurrent parent R（Make female This）Continue to be returned to obtain 3 generation BC of backcrossing respectively3F1-A、BC3F1-B、BC3F1-C、BC3F1-D、BC3F1-E、BC3F1-F；
7) above step 5 is repeated）,6）To 4 generation BC of backcrossing4F1In generation, plants BC4F1In generation, obtains BC4F2Generation, BC4F2-D、BC4F2- E、BC4F2The pest-resistant disease resistance of 3 combinations such as-F is identified that its corresponding resistant parent institute Resistant kind is only identified in each combination, Highly resistance is screened from each combination and agronomy and 3 maturity periods of yield traits and single plant similar in recurrent parent R harvest seed （BC4F2）, to BC4F2-A、BC4F2-B、BC4F2The kernel seed coat colour of-C soya seeds is screened, wherein BC4F2- A selection performances The seed of black character 15, BC4F2The seed 15 of the green character of-B selection performances, BC4F2- C selection performance brown characters Seed 15；
8) plantation step 7）Screen the BC of gained4F2-A、BC4F2-B、BC4F2- C seeds and BC4F2-D、BC4F2-E、BC4F2- F seeds, florescence investigate each strain and select economical character in each strain and recurrent parent R is closest, character of blooming Each 2 of single plant good, yield traits are excellent, makees hybridization BC4F2-A/BC4F2-D、BC4F2-B/ BC4F2-E、BC4F2-C/ BC4F2- F, must reestablish diplomatic relations F1 generation, with F1-AD、F1-BE、F1- CF is indicated；
9) F is planted1-AD、F1-BE、F1- CF, respectively sowing obtain F2-AD、F2-BE、F2- CF, to the kernel seed coat colour of seed into Row screening, F2The seed 50 of-AD selection performance black characters, F2The seed 50 of the green character of-BE selection performances, F2- CF is selected Select the seed 50 of performance brown character, the F of plantation screening gained2-AD、F2-BE、F2- CF, the pest-resistant disease resistance that 3 are combined Identified, its corresponding resistant parent institute Resistant kind is only identified in each combination, screen highly resistance from each combination and agronomy and Yield traits mix sowing for 5 with single plant similar in recurrent parent R, obtain F3-AD、F3-BE、F3-CF；
10) step 9 is repeated）To F4In generation, obtains F4-AD、F4-BE、F4- CF plants F4-AD、F4-BE、F4- CF, it is each to combine Screen agronomy and yield traits it is excellent, and with single plant similar in recurrent parent R 5 mix sowing, that is, be bred as economical character with take turns It returns parent R not detach unanimously, yield is high and is respectively provided with the anti-heart-eating worm of Black seed coat, green kind of skin resistance to SCN, brown The soybean near isogenic lines of the kind anti-graywall of skin is respectively designated as black anti-1, green anti-1, brown anti-1；
11) black anti-1, green anti-1, brown anti-1 distinguishes breeding seed with recurrent parent R, and the seed of breeding presses 1:1:1:1 or 1:2: 2:1 mixes up to the seed of the colored highly resistance soybean varieties of production, which is by 4 genetic background phases Closely, the near isogenic lines containing different kernel seed coat colours and resistant gene mixes in proportion, soybean near isogenic lines plantation Leaf morphology and Agronomic neat and consistent afterwards, yield is high, better resistance, but its soybean harvested contains black soybean, blueness Soybean, brown soybean, soybean.
Advantageous effect
The beneficial effects of the present invention are respectively control black, green, brown seed coat color, highly resistance heart-eating worm, cyst roundworm Disease, grey speck of soybean gene by hybridization, be returned, breeding method of reestablishing diplomatic relations imports in same kind, be bred as genetic background it is identical, Near isogenic lines containing different kernel seed coat colours and Resistant Gene, these near isogenic lines leaf morphology, economical character and yield Close, the seed mixed in proportion plants not only 3 kinds of pest and disease damages of highly resistance, and plants beans plant shape state out and height expansion homogeneous It causes, produces the colored soybean that the seed of harvest mixes for black soybean, green soybean, brown soybean, soybean.Breeding through the invention Method generates " highly resistance colour soybean " seed, and colored soybean can be produced by planting the seed.The inventive method is simple, efficiently, can Row, the highly resistance colour soybean varieties production method of invention is simple, better resistance, yield are high, efficient, profitable, production method section Process and cost are saved, and the colored soybean produced is rich in a variety of natural nutrition elements and physiological activator, often feeding can be promoted strong Health adjusts body's physiological rhythm.The colour soybean price is high, plants the colour soybean varieties and increases receipts than common variety per acre Enter 600-800 members, high financial profit, wide market.
Specific implementation mode：
Embodiment 1：
1) high yield and high quality highly resistance soybean varieties Ji agriculture 40 is selected to make recurrent parent, respectively with highly resistance heart-eating worm soybean varieties Jilin No. 16（P1）, the high anti-line 1 of resistance to SCN soybean varieties（P2）, highly resistance grey speck of soybean kind close rich 29（P3）, it is black big Beans kind dragon black soybean 2（P4）, green soybean kind north agriculture 106（P5）, brown soybean kind is bright educates 0305（P6）Hybridization, P1, P2, P3, P4, P5, P6 make male parent, hybridize to obtain hybridization F1In generation, is respectively with F1-A、F1-B、F1-C、F1-D、F1-E、F1- F is indicated；
2) plantation hybridization F1For seed F1-A、F1-B、F1-C、F1-D、F1-E、F1Each 5 plants of-F, florescence is respectively therefrom selected excellent Single plant 2 makees female parent with recurrent parent and is returned to obtain 1 generation BC of backcrossing respectively1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、 BC1F1-F；
3) 1 generation BC of plantation backcrossing1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、BC1F1- F seeds, maturity period receive Obtain seed（BC1F2）, to BC1F1-A、BC1F1-B、BC1F1The kernel seed coat colour of-C soya seeds is screened, wherein BC1F2- A is selected Select the seed 20 of performance Black seed coat character, BC1F2The seed 20 of green kind of the skin character of-B selection performances, BC1F2- C is selected Show the seed 20 of brown seed coat color character；
4) BC that plantation screening obtains1F2-A、BC1F2-B、BC1F2- C seeds and without screening to obtain BC1F2-D、BC1F2-E、 BC1F2- F seeds, to BC1F2-D、BC1F2-E、BC1F2The pest-resistant disease resistance of 3 combinations such as-F is identified that each combination is only reflected Its fixed corresponding resistant parent institute Resistant kind, screens highly resistance from each combination and agronomy and yield traits is close with recurrent parent Single plant 3 make female parent with recurrent parent and continue to be returned respectively, BC1F2-A、BC1F2-B、BC1F2- C strains also select agronomy and production Amount character is close with recurrent parent and each 3 of the good elite seedling of character of blooming makees female parent with recurrent parent and continues to be returned respectively It is returned 2 generation BC2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1-F；
5) 2 generation BC of plantation backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1- F seeds, maturity period receive Obtain seed（BC2F2）, to BC2F2-A、BC2F2-B、BC2F2The kernel seed coat colour of-C soya seeds is screened, wherein BC2F2- A is selected Select the seed 20 of performance black character, BC2F2The seed 20 of the green character of-B selection performances, BC2F2- C selection performance brown The seed of character 20；
6) BC that plantation screening obtains2F2Strain BC2F2-A、BC2F2-B、BC2F2- C seeds and without screening to obtain BC2F2-D、 BC2F2-E、BC2F2- F seeds, to BC2F2-D、BC2F2-E、BC2F2The pest-resistant disease resistances of the combination of-F etc. 3 are identified, each Its corresponding resistant parent institute Resistant kind is only identified in combination, screening highly resistance and agronomy and yield traits and samsara from each combination Single plant 3 and recurrent parent similar in parent（Make maternal）Continue to be returned respectively, BC2F2-A、BC2F2-B、BC2F2- C strains are also selected Select that agronomy and yield traits are close with recurrent parent and bloom each 3 of the good elite seedling of character and recurrent parent R（Make maternal） Continue to be returned to obtain 3 generation BC of backcrossing respectively3F1-A、BC3F1-B、BC3F1-C、BC3F1-D、BC3F1-E、BC3F1-F；
7) above step 5 is repeated）,6）To 4 generation BC of backcrossing4F1In generation, plants BC4F1In generation, obtains BC4F2Generation, BC4F2-D、BC4F2- E、BC4F2The pest-resistant disease resistance of 3 combinations such as-F is identified that its corresponding resistant parent institute Resistant kind is only identified in each combination, Highly resistance is screened from each combination and agronomy and 3 maturity periods of yield traits and single plant similar in recurrent parent R harvest seed （BC4F2）, to BC4F2-A、BC4F2-B、BC4F2The kernel seed coat colour of-C soya seeds is screened, wherein BC4F2- A selection performances The seed of black character 15, BC4F2The seed 15 of the green character of-B selection performances, BC4F2- C selection performance brown characters Seed 15；
8) plantation step 7）Screen the BC of gained4F2-A、BC4F2-B、BC4F2- C seeds and BC4F2-D、BC4F2-E、BC4F2- F seeds, florescence investigate each strain and select economical character in each strain and recurrent parent R is closest, character of blooming Each 2 of single plant good, yield traits are excellent, makees hybridization BC4F2-A/BC4F2-D、BC4F2-B/ BC4F2-E、BC4F2-C/ BC4F2- F, must reestablish diplomatic relations F1 generation, with F1-AD、F1-BE、F1- CF is indicated；
9) F is planted1-AD、F1-BE、F1- CF, respectively sowing obtain F2-AD、F2-BE、F2- CF, to the kernel seed coat colour of seed into Row screening, F2The seed 50 of-AD selection performance black characters, F2The seed 50 of the green character of-BE selection performances, F2- CF is selected Select the seed 50 of performance brown character, the F of plantation screening gained2-AD、F2-BE、F2- CF, the pest-resistant disease resistance that 3 are combined Identified, its corresponding resistant parent institute Resistant kind is only identified in each combination, screen highly resistance from each combination and agronomy and Yield traits mix sowing for 5 with single plant similar in recurrent parent R, obtain F3-AD、F3-BE、F3-CF；
10) step 9 is repeated）To F4In generation, obtains F4-AD、F4-BE、F4- CF plants F4-AD、F4-BE、F4- CF, it is each to combine Screen agronomy and yield traits it is excellent, and with single plant similar in recurrent parent R 5 mix sowing, that is, be bred as economical character with take turns It returns parent R not detach unanimously, yield is high and is respectively provided with the anti-heart-eating worm of Black seed coat, green kind of skin resistance to SCN, brown The soybean near isogenic lines of the kind anti-graywall of skin is respectively designated as black anti-1, blueness anti-1, brown anti-1；
11) black anti-1, blueness anti-1, brown anti-1 and recurrent parent R difference breeding seeds, the seed of breeding press 1:1:1:1 is mixed It closes up to the seed of the colored highly resistance soybean varieties of production, which is by 4 similar genetic backgrounds, contains The near isogenic lines of different kernel seed coat colours and resistant gene mixes in proportion, and strain is leaf after soybean near isogenic lines plantation State and Agronomic neat and consistent, yield are high, better resistance, but its soybean harvested contains black soybean, green soybean, brown big Beans, soybean.
Embodiment 2：
1) high yield and high quality highly resistance soybean varieties Ji agriculture 40 is selected（It is represented with parent R）Make recurrent parent, eats the heart with highly resistance respectively Worm soybean varieties Jilin 16（P1）, the high anti-line 1 of resistance to SCN soybean varieties（P2）, highly resistance grey speck of soybean kind close Rich 29（P3）, black soybean kind south agriculture 26（P4）, green soybean kind Shanxi product 1（P5）, brown soybean kind is bright educates 0305（P6）It is miscellaneous It hands over, P1, P2, P3, P4, P5, P6 make male parent, hybridize to obtain hybridization F1In generation, is respectively with F1-A、F1-B、F1-C、F1-D、F1-E、F1- F tables Show；
2) plantation hybridization F1For seed F1-A、F1-B、F1-C、F1-D、F1-E、F1Each 5 plants of-F, florescence is respectively therefrom selected excellent Single plant 2 and recurrent parent R（Make maternal）It is returned to obtain 1 generation BC of backcrossing respectively1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、 BC1F1-E、BC1F1-F；
3) 1 generation BC of plantation backcrossing1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、BC1F1- F seeds, maturity period receive Obtain seed（BC1F2）, to BC1F1-A、BC1F1-B、BC1F1The kernel seed coat colour of-C soya seeds is screened, wherein BC1F2- A is selected Select the seed 20 of performance Black seed coat character, BC1F2The seed 20 of green kind of the skin character of-B selection performances, BC1F2- C is selected Show the seed 20 of brown seed coat color character；
4) BC that plantation screening obtains1F2-A、BC1F2-B、BC1F2- C seeds and without screening to obtain BC1F2-D、BC1F2-E、 BC1F2- F seeds, to BC1F2-D、BC1F2-E、BC1F2The pest-resistant disease resistance of 3 combinations such as-F is identified that each combination is only reflected Its fixed corresponding resistant parent institute Resistant kind, screening highly resistance and agronomy and yield traits and recurrent parent R phases from each combination Close single plant 3 and recurrent parent（Make maternal）Continue to be returned respectively, BC1F2-A、BC1F2-B、BC1F2- C strains also select agronomy And bloom character good elite seedling each 3 and recurrent parent R close with recurrent parent R with yield traits（Make maternal）Respectively after It is continuous to be returned to obtain 2 generation BC of backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1-F；
5) 2 generation BC of plantation backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1- F seeds, maturity period receive Obtain seed（BC2F2）, to BC2F2-A、BC2F2-B、BC2F2The kernel seed coat colour of-C soya seeds is screened, wherein BC2F2- A is selected Select the seed 20 of performance black character, BC2F2The seed 20 of the green character of-B selection performances, BC2F2- C selection performance brown The seed of character 20；
6) BC that plantation screening obtains2F2Strain BC2F2-A、BC2F2-B、BC2F2- C seeds and without screening to obtain BC2F2-D、 BC2F2-E、BC2F2- F seeds, to BC2F2-D、BC2F2-E、BC2F2The pest-resistant disease resistances of the combination of-F etc. 3 are identified, each Its corresponding resistant parent institute Resistant kind is only identified in combination, screening highly resistance and agronomy and yield traits and samsara from each combination Single plant 3 and recurrent parent similar in parent R（Make maternal）Continue to be returned respectively, BC2F2-A、BC2F2-B、BC2F2- C strains Selection agronomy and yield traits are close with recurrent parent R and bloom each 3 of the good elite seedling of character and recurrent parent R（Make female This）Continue to be returned to obtain 3 generation BC of backcrossing respectively3F1-A、BC3F1-B、BC3F1-C、BC3F1-D、BC3F1-E、BC3F1-F；
7) above step 5 is repeated）,6）To 4 generation BC of backcrossing4F1In generation, plants BC4F1In generation, obtains BC4F2Generation, BC4F2-D、BC4F2- E、BC4F2The pest-resistant disease resistance of 3 combinations such as-F is identified that its corresponding resistant parent institute Resistant kind is only identified in each combination, Highly resistance is screened from each combination and agronomy and 3 maturity periods of yield traits and single plant similar in recurrent parent R harvest seed （BC4F2）, to BC4F2-A、BC4F2-B、BC4F2The kernel seed coat colour of-C soya seeds is screened, wherein BC4F2- A selection performances The seed of black character 15, BC4F2The seed 15 of the green character of-B selection performances, BC4F2- C selection performance brown characters Seed 15；
8) plantation step 7）Screen the BC of gained4F2-A、BC4F2-B、BC4F2- C seeds and BC4F2-D、BC4F2-E、BC4F2- F seeds, florescence investigate each strain and select economical character in each strain and recurrent parent R is closest, character of blooming Each 2 of single plant good, yield traits are excellent, makees hybridization BC4F2-A/BC4F2-D、BC4F2-B/ BC4F2-E、BC4F2-C/ BC4F2- F, must reestablish diplomatic relations F1 generation, with F1-AD、F1-BE、F1- CF is indicated；
9) F is planted1-AD、F1-BE、F1- CF, respectively sowing obtain F2-AD、F2-BE、F2- CF, to the kernel seed coat colour of seed into Row screening, F2The seed 50 of-AD selection performance black characters, F2The seed 50 of the green character of-BE selection performances, F2- CF is selected Select the seed 50 of performance brown character, the F of plantation screening gained2-AD、F2-BE、F2- CF, the pest-resistant disease resistance that 3 are combined Identified, its corresponding resistant parent institute Resistant kind is only identified in each combination, screen highly resistance from each combination and agronomy and Yield traits mix sowing for 5 with single plant similar in recurrent parent R, obtain F3-AD、F3-BE、F3-CF；
10) step 9 is repeated）To F4In generation, obtains F4-AD、F4-BE、F4- CF plants F4-AD、F4-BE、F4- CF, it is each to combine Screen agronomy and yield traits it is excellent, and with single plant similar in recurrent parent R 5 mix sowing, that is, be bred as economical character with take turns It returns parent R not detach unanimously, yield is high and is respectively provided with the anti-heart-eating worm of Black seed coat, green kind of skin resistance to SCN, brown The soybean near isogenic lines of the kind anti-graywall of skin is respectively designated as black anti-2, blueness anti-2, brown anti-2；
11）Black anti-2, blueness anti-2, brown anti-2 and recurrent parent R distinguishes breeding seed, and the seed of breeding presses 1:2:2:1 is mixed It closes up to the seed of the colored highly resistance soybean varieties of production, which is by 4 similar genetic backgrounds, contains The near isogenic lines of different kernel seed coat colours and resistant gene mixes in proportion, and strain is leaf after soybean near isogenic lines plantation State and Agronomic neat and consistent, yield are high, better resistance, but its soybean harvested contains black soybean, green soybean, brown big Beans, soybean.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification should all belong to the covering scope of the present invention.

Claims (1)

1. a kind of selection of highly resistance colour soybean varieties, it is characterised in that：The soybean varieties strain of the selection selection and breeding Leaf pattern neat and consistent, character stablize heritable, highly resistance heart-eating worm, cyst nematode disease and grey speck of soybean, the colour produced Soybean contains the soybean of black soybean, green soybean, 4 kinds of soybean, brown soybean colors；The selection includes the following steps：
1）Select high yield and high quality highly resistance soybean varieties Ji agriculture 40 with parent's R masterpiece recurrent parents, respectively with highly resistance heart-eating worm soybean - the P1 of kind Jilin 16, No. 1-P2 of the anti-line of high resistance to SCN soybean varieties, highly resistance grey speck of soybean kind close rich 29-P3, Black soybean kind dragon black soybean 2 or No. 1 southern agriculture 26-P4, green soybean kind north agriculture 106 or Shanxi product-P5, brown soybean kind is bright educates 0305-P6 hybridizes, and P1, P2, P3, P4, P5, P6 make male parent, hybridizes to obtain hybridization F1In generation, is respectively with F1-D、F1-E、F1-F、F1-A、F1- B、F1- C is indicated；
2）Plantation hybridization F1For seed F1-A、F1-B、F1-C、F1-D、F1-E、F1Each 5 plants of-F, florescence respectively therefrom selects elite seedling 2 It is a to be returned to obtain 1 generation BC of backcrossing respectively with recurrent parent R that is making female parent1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、 BC1F1-F；
3）1 generation BC of plantation backcrossing1F1-A、BC1F1-B、BC1F1-C、BC1F1-D、BC1F1-E、BC1F1- F seeds, maturity period harvest kind Sub- BC1F2, to BC1F1-A、BC1F1-B、BC1F1The soya seeds BC of-C harvests1F2Kernel seed coat colour screened, wherein BC1F2- The seed 20 of A selection performance Black seed coat characters, BC1F2The seed 20 of green kind of the skin character of-B selection performances, BC1F2-C The seed 20 of selection performance brown seed coat color character；
4）The BC that plantation screening obtains1F2-A、BC1F2-B、BC1F2- C seeds and without screening to obtain BC1F2-D、BC1F2-E、BC1F2- F seeds, to BC1F2-D、BC1F2-E、BC1F2The pest-resistant disease resistance of 3 combinations such as-F is identified that its phase is only identified in each combination Resistant parent institute Resistant kind is answered, screening highly resistance and agronomy and yield traits and list similar in recurrent parent R from each combination Strain 3 continues to be returned respectively with maternal recurrent parent is made, BC1F2-A、BC1F2-B、BC1F2- C strains also select agronomy and yield Character is close with recurrent parent R and each 3 of the good elite seedling of character of blooming continues to be returned respectively with maternal recurrent parent R is made 2 generation BC must be returned2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1-F；
5）2 generation BC of plantation backcrossing2F1-A、BC2F1-B、BC2F1-C、BC2F1-D、BC2F1-E、BC2F1- F seeds, maturity period harvest kind Sub- BC2F2, to BC2F2-A、BC2F2-B、BC2F2The kernel seed coat colour of-C soya seeds is screened, wherein BC2F2- A selection performances The seed of black character 20, BC2F2The seed 20 of the green character of-B selection performances, BC2F2- C selection performance brown characters Seed 20；
6）The BC that plantation screening obtains2F2Strain BC2F2-A、BC2F2-B、BC2F2- C seeds and without screening to obtain BC2F2-D、 BC2F2-E、BC2F2- F seeds, to BC2F2-D、BC2F2-E、BC2F2The pest-resistant disease resistances of the combination of-F etc. 3 are identified, each Its corresponding resistant parent institute Resistant kind is only identified in combination, screening highly resistance and agronomy and yield traits and samsara from each combination Single plant 3 similar in parent R continues to be returned respectively with maternal recurrent parent is made, BC2F2-A、BC2F2-B、BC2F2- C strains Selection agronomy and the samsara parent that yield traits are close with recurrent parent R and each 3 of the good elite seedling of character of blooming is maternal with work This R continues to be returned to obtain 3 generation BC of backcrossing respectively3F1-A、BC3F1-B、BC3F1-C、BC3F1-D、BC3F1-E、BC3F1-F；
7）Repeat above step 5）,6）To 4 generation BC of backcrossing4F1In generation, plants BC4F1In generation, obtains BC4F2Generation, BC4F2-D、BC4F2-E、 BC4F2The pest-resistant disease resistance of 3 combinations such as-F is identified that its corresponding resistant parent institute Resistant kind is only identified in each combination, from 3 maturity period harvest seed BC of screening highly resistance and agronomy and yield traits and single plant similar in recurrent parent R in each combination4F2, To BC4F2-A、BC4F2-B、BC4F2The kernel seed coat colour of-C soya seeds is screened, wherein BC4F2- A selection performance black characters Seed 15, BC4F2The seed 15 of the green character of-B selection performances, BC4F2The seed 15 of-C selection performance brown characters Grain；
8）Plantation step 7）Screen the BC of gained4F2-A、BC4F2-B、BC4F2- C seeds and BC4F2-D、BC4F2-E、BC4F2- F kinds Son, florescence investigate each strain and select economical character in each strain and recurrent parent R is closest, character of blooming is good, Each 2 of the excellent single plant of yield traits, makees hybridization BC4F2-A/BC4F2-D、BC4F2-B/ BC4F2-E、BC4F2-C/BC4F2- F, Must reestablish diplomatic relations F1 generation, with F1-AD、F1-BE、F1- CF is indicated；
9）Plant F1-AD、F1-BE、F1- CF, respectively sowing obtain F2-AD、F2-BE、F2- CF sieves the kernel seed coat colour of seed Choosing, F2The seed 50 of-AD selection performance black characters, F2The seed 50 of the green character of-BE selection performances, F2- CF selects table The seed 50 of existing brown character, the F of plantation screening gained2-AD、F2-BE、F2- CF, the pest-resistant disease resistance combined to 3 carry out Its corresponding resistant parent institute Resistant kind is only identified in identification, each combination, screening highly resistance and agronomy and yield from each combination Character mixes sowing for 5 with single plant similar in recurrent parent R, obtains F3-AD、F3-BE、F3-CF；
10）Repeat step 9）To F4In generation, obtains F4-AD、F4-BE、F4- CF plants F4-AD、F4-BE、F4- CF, each combined sorting agriculture Skill and yield traits are excellent, and with single plant similar in recurrent parent R 5 mix sowing, that is, be bred as economical character and recurrent parent R It does not detach unanimously, yield is high and is respectively provided with the anti-heart-eating worm of Black seed coat, green kind of skin resistance to SCN, the anti-ash of brown seed coat color The soybean near isogenic lines of pinta is respectively designated as black anti-1, green anti-1, brown anti-1；
11）Black anti-1, green anti-1, brown anti-1 distinguishes breeding seed with recurrent parent R, and the seed of breeding presses 1:1:1:1 or 1:2:2:1 It mixes up to the seed of the colored highly resistance soybean varieties of production, which is by 4 similar genetic backgrounds, contains There is the near isogenic lines of different kernel seed coat colours and resistant gene to mix in proportion, strain leaf after soybean near isogenic lines plantation Form and Agronomic neat and consistent, yield are high, better resistance, but its soybean harvested contains black soybean, green soybean, brown Soybean, soybean.
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Publication number Priority date Publication date Assignee Title
US6350937B1 (en) * 2000-06-22 2002-02-26 Kevin Threlkeld Soybean cultivar M713612
CN101204137A (en) * 2006-12-18 2008-06-25 中国科学院东北地理与农业生态研究所 Selecting method for wide adaptable, high-yielded and adverse-resistant soybean varieties
CN102630557A (en) * 2012-05-12 2012-08-15 福建农林大学 Method for breeding functional formula rice varieties

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350937B1 (en) * 2000-06-22 2002-02-26 Kevin Threlkeld Soybean cultivar M713612
CN101204137A (en) * 2006-12-18 2008-06-25 中国科学院东北地理与农业生态研究所 Selecting method for wide adaptable, high-yielded and adverse-resistant soybean varieties
CN102630557A (en) * 2012-05-12 2012-08-15 福建农林大学 Method for breeding functional formula rice varieties

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我国培育成功彩色大豆;无;《广西热带农业》;20041231(第5期);24 *

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