CN103229713B - Selective breeding method for high-nutrition rice varieties - Google Patents

Selective breeding method for high-nutrition rice varieties Download PDF

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CN103229713B
CN103229713B CN201310162309.6A CN201310162309A CN103229713B CN 103229713 B CN103229713 B CN 103229713B CN 201310162309 A CN201310162309 A CN 201310162309A CN 103229713 B CN103229713 B CN 103229713B
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lysine
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iron
cellulose
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CN103229713A (en
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章清杞
陈志伟
陈幼玉
黄荣华
李美德
杨帆
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Luoning City Fengzhi Changshun Science and Technology Co., Ltd.
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Fujian Agriculture and Forestry University
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Abstract

The invention discloses a selective breeding method for high-nutrition rice varieties, which belongs to the technical field of special rice seed breeding. The selective breeding method disclosed by the invention comprises the following steps of: selecting high-yield, high-quality and disease-resistant rice varieties as recurrent parents; hybridizing the recurrent parents with iron-rich rice variety IR164, high-sugar rice variety Bai Tian No.1, high-lysine rice variety Milyang 164 and high-cellulose rice variety water source 464 respectively; performing backcross and convergent cross for times; and breeding the high-nutrition rice varieties having economical characters and yield characters which are similar to the economical characters and yield characters of the recurrent parents, and rich in the nutritional ingredients of iron, sugar, lysine, cellulose and the like by selecting the economical characters, yield characters, iron content, sugar content, lysine content, cellulose content and the like of separating generations.

Description

A kind of selection of high nutrition rice kind
Technical field
The present invention is specifically related to a kind of selection of high nutrition rice kind, belongs to special rice breeding technical field.
Background technology
In recent years, the alimentary health-care function of rice is more and more subject to consumer's attention, and some special rices have the extremely consumer favor of unique health-care efficacy to human body because of some contained functional components.As international paddy rice has been bred as the rich iron high-yield rice kind IR164 that iron content exceedes 25mg/kg, the breeding man of Switzerland has been bred as " golden rice " kind (Goldenrice) that is rich in vitamin A, breeding man of Korea S has been bred as sweet rice hwachengbyeo su-1, hwachengbyeo su-2 (total sugar content is up to 5%-9%), opaque rice hwachengbyeofro, soft rice, high-lysine rice varieties Milyang164, high-cellulose kind " water source 464 " (dietary fiber content of brown rice and polished rice is higher 2 times and 3 times than contrast respectively), the Kumamaru T of breeding man of Japan brings out 10 high-lysine mutation bodies that lysine content is 5.10%-6.38%, Rice Inst., Jiling Academy of Agricultural Science has cultivated the sticky Jilin of the nutritious black perfume (or spice) of round-grained rice type black rice dragon brocade No. one and red fragrant No. one, red perfume (or spice) is glutinous, delicate fragrance giant embryo is glutinous, black sweet No. one, red sweet No. one, No. Bai Tianyi, sweet glutinous No. one, a series of Special Rices new lines such as black glutinous No. one.These Special Rices strains have different nutrition and health care functions because being rich in different special dietary compositions.These functional rice products have wide market prospects and exploitation is worth, its market price is higher 2 to 5 times than common rice, popularizing planting, for the income that improves vast kind of rice peasant, for the trophic level that improves broad masses of the people, improves health and all has great importance.
But, the kind of report is and is rich in 1-2 kind functional component at present, the rice varieties that is rich in several functions composition is comparatively rare, and the high nutrition rice kind that is particularly simultaneously rich in 4 kinds of nutrient components such as iron (needed by human body trace element), sugar (improving mouthfeel), lysine (essential amino acid) and cellulose (improving functions of intestines and stomach) there is not yet report.
Summary of the invention
The object of the present invention is to provide a kind of selection of high nutrition rice kind, utilize the method can select the high nutrition rice kind of nutrient components such as being rich in iron, sugar, lysine and cellulose.
For reaching above object, the present invention by the following technical solutions:
1) selecting high yield and high quality disease resisting rice kind is recurrent parent R, take iron-rich rice kind N1, high syrup rice varieties N2, high-lysine rice varieties N3, high-cellulose rice varieties N4 as non-recurrent parent, make hybridization R/N1, R/N2, R/N3, R/N4, must hybridize F 1in generation,, respectively with F 1-N1, F 1-N2, F 1-N3, F 1-N4 represents;
2) plantation hybridization F 1in generation, make hybridization F flowering stage 1-N1/F 1-N2 and F 1-N3/F 1-N4, F must reestablish diplomatic relations 1in generation,, respectively with F 1-N12, F 1-N34 represents;
3) plantation F 1-N12, F 1-N34, R/F backcrosses flowering stage 1-N12 and R/F 1-N34, in 1 generation that must backcross,, respectively with BC 1f 1-N12, BC 1f 1-N34 represents;
4) plantation BC 1f 1-N12, BC 1f 1-N34, selfing obtains F 2for BC 1f 2-N12, BC 1f 2-N34;
5) plantation F 2for BC 1f 2-N12, BC 1f 2-N34, maturing stage individual plant results seed, respectively with BC 1f 3-N12 and BC 1f 3-N34 represents, carries out nutrient component determining, BC 1f 3-N12 measures iron and sugar content, BC 1f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 1f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 1f 310 of-N34 individual plants;
6) plantation 5) BC that obtains of screening 1f 3-N12 and BC 1f 3-N34, investigates each strain flowering stage and selects 5 of the individual plants that economical character, yield traits are close with recurrent parent R to continue respectively to backcross obtaining 2 generations that backcrossed with recurrent parent R, respectively with BC 2f 1-N12, BC 2f 1-N34 represents;
7) plantation 6) BC that backcrosses and obtain 2f 1-N12, BC 2f 1-N34, selfing obtains BC 2f 2-N12, BC 2f 2-N34;
8) plantation 7) BC that obtains 2f 2-N12, BC 2f 2-N34, maturing stage individual plant results seed, respectively with BC 2f 3-N12, BC 2f 3-N34 represents, carries out nutrient component determining, BC 2f 3-N12 measures iron and sugar content, BC 2f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 2f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 2f 310 of-N34 individual plants;
9) plantation 8) BC that obtains of screening 2f 3-N12, BC 2f 3-N34, investigates each strain flowering stage and selects 1 of the individual plant that economical character, yield traits are the most close with recurrent parent R to make hybridization BC 2f 3-N12/ BC 2f 3-N34, the F1 generation of must reestablishing diplomatic relations, with BC 2f 3-N1234 represents;
10) plantation 9) BC that obtains of screening 2f 3-N1234, selfing obtains its F 2for BC 2f 4-N1234;
11) plantation 10) BC that obtains of screening 2f 4-N1234, investigate each strain flowering stage and select economical character, individual plant 200 strain sowings that yield traits is close with recurrent parent R, measure iron, sugar, lysine and content of cellulose, screen all 10 of high individual plants of 4 kinds of nutrient composition contents, the screening for economical character, yield traits through 1-2 again, be bred as economical character and yield traits close with recurrent parent R, proterties is stable not to be separated, and is rich in iron, sugar, lysine and cellulosic high nutrition rice kind.
Described recurrent parent R accounts for or faces rice No. 10 for Huang is soft.
Take iron-rich rice kind N1:IR164, high syrup rice varieties N2: No. Bai Tianyi, high-lysine rice varieties N3:Milyang164, high-cellulose rice varieties N4: water source 464 is as non-recurrent parent.
Beneficial effect of the present invention:
The present invention is aggregated to the proterties of high Fe content, high sugar content, high-lysine and content of cellulose in high yield, high-quality, disease-resistant good rice varieties by hybridization, back cross breeding method respectively, be bred as economical character close with former improved seeds with yield traits, but the high trophic function Special rice kind of the nutrient components such as rich iron, sugar, lysine and cellulose, the rice mouthfeel of this variety production is good and be rich in physiological activator, edible can promote healthy, regulate function of human body, there is good nutritional health function.
Embodiment
Embodiment 1:
1) select yellow soft accounting for as recurrent parent (R), with iron-rich rice kind IR164(N1) (Hu Peisong. functional rice research and development. Chinese rice, 2003, 5:3-5), No. Bai Tianyi (N2) (Nan Zhonghao of high syrup rice varieties, full Dongxing, Zhou Jian, Deng. the feature of Jilin Special Rices and exploitation. Yanbian University's agricultural journal, 2000, 22 (1): 60-62), high-lysine rice varieties Milyang164(N3) (Li Shengyuan, Jin Shengnan, Piao Zhongze, Deng. the research of Korea S's special rice with utilize status and prospects. Shanghai Agricultural journal 2002, 18 (supplementary issue): 47-52), high-cellulose rice varieties water source 464(N4) (Li Shengyuan, Jin Shengnan, Piao Zhongze, Deng. the research of Korea S's special rice with utilize status and prospects. Shanghai Agricultural journal 2002, 18 (supplementary issue): 47-52) be non-recurrent parent, make hybridization R/N1, R/N2, R/N3, R/N4, must hybridize F 1in generation,, respectively with F 1-N1, F 1-N2, F 1-N3, F 1-N4 represents,
2) plantation F 1-N1, F 1-N2, F 1-N3, F 1-N4 seed, makes hybridization F flowering stage 1-N1/F 1-N2 and F 1-N3/F 1-N4, F must reestablish diplomatic relations 1in generation,, respectively with F 1-N12, F 1-N34 represents;
3) plantation F 1-N12, F 1-N34, R/F backcrosses flowering stage 1-N12 and R/F 1-N34, in 1 generation that must backcross,, respectively with BC 1f 1-N12, BC 1f 1-N34 represents;
4) plantation BC 1f 1-N12, BC 1f 1-N34, selfing obtains F 2for BC 1f 2-N12, BC 1f 2-N34;
5) plantation F 2for BC 1f 2-N12, BC 1f 2-N34, the maturing stage, individual plant results seed was (with BC 1f 3-N12 and BC 1f 3-N34 represents), carry out nutrient component determining, BC 1f 3-N12 measures iron and sugar content, BC 1f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 1f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 1f 310 of-N34 individual plants;
6) plantation 5) BC that obtains of screening 1f 3-N12 and BC 1f 3-N34, investigates each strain flowering stage and selects economical character, yield traits and yellow soft 5 of the close individual plants that account for to continue respectively to backcross obtaining 2 generations that backcrossed with recurrent parent R, respectively with BC 2f 1-N12, BC 2f 1-N34 represents;
7) plantation 6) BC that backcrosses and obtain 2f 1-N12, BC 2f 1-N34, selfing obtains BC 2f 2-N12, BC 2f 2-N34;
8) plantation 7) BC that obtains 2f 2-N12, BC 2f 2-N34, maturing stage individual plant results seed (BC 2f 3-N12, BC 2f 3-N34), carry out nutrient component determining, BC 2f 3-N12 measures iron and sugar content, BC 2f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 2f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 2f 310 of-N34 individual plants;
9) plantation 8) BC that obtains of screening 2f 3-N12, BC 2f 3-N34, investigates each strain flowering stage and selects economical character, yield traits and yellow soft 1 of the most close individual plant that accounts for to make hybridization BC 2f 3-N12/ BC 2f 3-N34, the F1 generation of must reestablishing diplomatic relations is with BC 2f 3-N1234 represents;
10) plantation 9) BC that obtains of screening 2f 3-N1234, selfing obtains its F 2for BC 2f 4-N1234;
11) plantation 10) BC that obtains of screening 2f 4-N1234, investigate each strain flowering stage and select economical character, yield traits and the yellow soft close individual plant 200 strain sowings that account for, measure iron, sugar, lysine and content of cellulose, screen all 10 of high individual plants of 4 kinds of nutrient composition contents, again through 1-2 for the screening of economical character, yield traits be bred as economical character and yield traits and yellow soft account for close, proterties is stable not to be separated, and is rich in the high nutrition rice kind " yellow soft the accounting for of high nutrition " of the nutrient components such as iron, sugar, lysine and cellulose.
Embodiment 2:
1) selecting to face rice is for No. 10 recurrent parent (R), take iron-rich rice kind IR164(N1), high syrup rice varieties No. Bai Tianyi (N2), high-lysine rice varieties Milyang164(N3), high-cellulose rice varieties water source 464(N4) as non-recurrent parent, make hybridization R/N1, R/N2, R/N3, R/N4, must hybridize F 1in generation,, respectively with F 1-N1, F 1-N2, F 1-N3, F 1-N4 represents;
2) plantation F 1-N1, F 1-N2, F 1-N3, F 1-N4 seed, makes hybridization F flowering stage 1-N1/F 1-N2 and F 1-N3/F 1-N4, F must reestablish diplomatic relations 1in generation,, respectively with F 1-N12, F 1-N34 represents;
3) plantation F 1-N12, F 1-N34, R/F backcrosses flowering stage 1-N12 and R/F 1-N34, in 1 generation that must backcross,, respectively with BC 1f 1-N12, BC 1f 1-N34 represents;
4) plantation BC 1f 1-N12, BC 1f 1-N34, selfing obtains F 2for BC 1f 2-N12, BC 1f 2-N34;
5) plantation F 2for BC 1f 2-N12, BC 1f 2-N34, the maturing stage, individual plant results seed was (with BC 1f 3-N12 and BC 1f 3-N34 represents), carry out nutrient component determining, BC 1f 3-N12 measures iron and sugar content, BC 1f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 1f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 1f 310 of-N34 individual plants;
6) plantation 5) BC that obtains of screening 1f 3-N12 and BC 1f 3-N34, investigates each strain flowering stage and selects economical character, yield traits and face 5 of the close individual plants of rice No. 10 and continue respectively to backcross obtaining 2 generations that backcrossed with recurrent parent R, respectively with BC 2f 1-N12, BC 2f 1-N34 represents;
7) plantation 6) BC that backcrosses and obtain 2f 1-N12, BC 2f 1-N34, selfing obtains BC 2f 2-N12, BC 2f 2-N34;
8) plantation 7) BC that obtains 2f 2-N12, BC 2f 2-N34, maturing stage individual plant results seed (BC 2f 3-N12, BC 2f 3-N34), carry out nutrient component determining, BC 2f 3-N12 measures iron and sugar content, BC 2f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 2f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 2f 310 of-N34 individual plants;
9) plantation 8) BC that obtains of screening 2f 3-N12, BC 2f 3-N34, investigates each strain flowering stage and selects economical character, yield traits and face 1 of the most close individual plant of rice No. 10 and make hybridization BC 2f 3-N12/ BC 2f 3-N34, the F1 generation of must reestablishing diplomatic relations is with BC 2f 3-N1234 represents;
10) plantation 9) BC that obtains of screening 2f 3-N1234, selfing obtains its F 2for BC 2f 4-N1234;
11) plantation 10) BC that obtains of screening 2f 4-N1234, investigate each strain flowering stage and select economical character, yield traits and face the close individual plant 200 strain sowings of rice No. 10, measure iron, sugar, lysine and content of cellulose, screen all 10 of high individual plants of 4 kinds of nutrient composition contents, be bred as economical character and yield traits through 1-2 for the screening of economical character, yield traits again and face rice No. 10 close, proterties is stable not to be separated, and is rich in the high nutrition rice kind " high nutrition is faced rice No. 10 " of the nutrient components such as iron, sugar, lysine and cellulose.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.

Claims (3)

1. a selection for high nutrition rice kind, is characterized in that: the concrete steps of described selection are as follows:
1) selecting high yield and high quality disease resisting rice kind is recurrent parent R, take iron-rich rice kind N1, high syrup rice varieties N2, high-lysine rice varieties N3, high-cellulose rice varieties N4 as non-recurrent parent, make hybridization R/N1, R/N2, R/N3, R/N4, must hybridize F 1in generation,, respectively with F 1-N1, F 1-N2, F 1-N3, F 1-N4 represents;
2) plantation hybridization F 1in generation, make hybridization F flowering stage 1-N1/F 1-N2 and F 1-N3/F 1-N4, F must reestablish diplomatic relations 1in generation,, respectively with F 1-N12, F 1-N34 represents;
3) plantation F 1-N12, F 1-N34, R/F backcrosses flowering stage 1-N12 and R/F 1-N34, in 1 generation that must backcross,, respectively with BC 1f 1-N12, BC 1f 1-N34 represents;
4) plantation BC 1f 1-N12, BC 1f 1-N34, selfing obtains F 2for BC 1f 2-N12, BC 1f 2-N34;
5) plantation F 2for BC 1f 2-N12, BC 1f 2-N34, maturing stage individual plant results seed, respectively with BC 1f 3-N12 and BC 1f 3-N34 represents, carries out nutrient component determining, BC 1f 3-N12 measures iron and sugar content, BC 1f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 1f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 1f 310 of-N34 individual plants;
6) plantation 5) BC that obtains of screening 1f 3-N12 and BC 1f 3-N34, investigates each strain flowering stage and selects 5 of the individual plants that economical character, yield traits are close with recurrent parent R to continue respectively to backcross obtaining 2 generations that backcrossed with recurrent parent R, respectively with BC 2f 1-N12, BC 2f 1-N34 represents;
7) plantation 6) BC that backcrosses and obtain 2f 1-N12, BC 2f 1-N34, selfing obtains BC 2f 2-N12, BC 2f 2-N34;
8) plantation 7) BC that obtains 2f 2-N12, BC 2f 2-N34, maturing stage individual plant results seed, respectively with BC 2f 3-N12, BC 2f 3-N34 represents, carries out nutrient component determining, BC 2f 3-N12 measures iron and sugar content, BC 2f 3-N34 measures lysine and content of cellulose, and screening retains all high BC of iron and sugar content 2f 310 of-N12 individual plants, screening retains all high BC of lysine and content of cellulose 2f 310 of-N34 individual plants;
9) plantation 8) BC that obtains of screening 2f 3-N12, BC 2f 3-N34, investigates each strain flowering stage and selects 1 of the individual plant that economical character, yield traits are the most close with recurrent parent R to make hybridization BC 2f 3-N12/ BC 2f 3-N34, the F1 generation of must reestablishing diplomatic relations, with BC 2f 3-N1234 represents;
10) plantation 9) BC that obtains of screening 2f 3-N1234, selfing obtains its F 2for BC 2f 4-N1234;
11) plantation 10) BC that obtains of screening 2f 4-N1234, investigate each strain flowering stage and select economical character, individual plant 200 strain sowings that yield traits is close with recurrent parent R, measure iron, sugar, lysine and content of cellulose, screen all 10 of high individual plants of 4 kinds of nutrient composition contents, the screening for economical character, yield traits through 1-2 again, be bred as economical character and yield traits close with recurrent parent R, proterties is stable not to be separated, and is rich in iron, sugar, lysine and cellulosic high nutrition rice kind.
2. the selection of a kind of high nutrition rice kind according to claim 1, is characterized in that: described recurrent parent R accounts for or faces rice No. 10 for Huang is soft.
3. the selection of a kind of high nutrition rice kind according to claim 1, is characterized in that: take iron-rich rice kind N1:IR164, high syrup rice varieties N2: No. Bai Tianyi, high-lysine rice varieties N3:Milyang164, high-cellulose rice varieties N4: water source 464 is as non-recurrent parent.
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