CN108496791A - Utilization method of the Rht3 indication laws in hybrid vigour - Google Patents
Utilization method of the Rht3 indication laws in hybrid vigour Download PDFInfo
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- CN108496791A CN108496791A CN201810230178.3A CN201810230178A CN108496791A CN 108496791 A CN108496791 A CN 108496791A CN 201810230178 A CN201810230178 A CN 201810230178A CN 108496791 A CN108496791 A CN 108496791A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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Abstract
The invention discloses a kind of utilization method of Rht3 indication laws in hybrid vigour, specially：Containing chain Rht3 mslb gene dwarf wheat strains it is that female parent is hybridized with ethereal blue grain indigo plant mark type dual purpose lines, is returned to cultivate, selects the stable excellent indigo plant grain dual purpose lines chain containing mslb Rht3 and ethereal blue grain dual purpose lines；With being that male parent is hybridized, is returned containing ethereal blue grain dual purpose lines chain mslb the Rht3 new varieties or new lines good with local general performance, F1 generation and the backcrossing fertility offspring of each male parent are obtained, selects plant height in the blue grain dual purpose lines strains of 75cm or so.The blue grain of the selection blue grain dual purpose lines strain new for isolation and selection next year.Final to obtain breeding wheat blue grain dual purpose lines of short stem, for white grain for hybrid seeding and breeding hybridized, blue grain is used for breeding male sterile lines.The present invention reduces the plant height of hybrid wheat, improves hybrid wheat kind ability resistant to lodging, reduces the risk of lodging, improves wheat yield.
Description
Technical field
The invention belongs to crops breeding technique fields, more particularly, to a kind of Rht3 indication laws in hybrid vigour
On utilization method.
Background technology
Wheat is that cultivated area is most wide in the world, the highest cereal crops of total output；There is 30%~40% people in the whole world
It is limited in cultivated area mouthful using wheat as main food, improve yield of wheat be improve grain yield it is important by way of
One of, thus quickly breeding high yield, good quality wheat new varieties are well stimulations the most efficiently, economic.
Breeding wheat for semidwarfness is to cultivate one of high yield, good quality wheat new varieties important measures.The application of dwarf gene achieves huge
Big achievement causes in the sixties in last century and thereafter one " green revolution ", the yield of cereal crops is made to be significantly increased,
In this " green revolution ", the breeding wheat for semidwarfness of wheat plays the role of key.Wheat has been reported and position name has 25, closely
Year, report had new dwarf gene to be found successively again, in this more than 20 a dwarf gene, with Rht1, Rht2, Rht8, Rht9
Most widely used in production Deng a small number of Recessive dwarf genes, the new varieties of selection and breeding are most, and have and centainly drop the dominant of short effect
Or semidominant dwarf gene (Rht3, Rht10, Rht12, Rht21 etc.) also sends out brightness important function not in production.
Have been reported that the short effect of the drop of indication law, the main Dominant Dwarfing Source of wheat reduce parent's plant height 15.0%~
43.8%, the middle advantage of plant height is -14.7%~3.8%, only No. a kind of short change of the gene containing Rht10, reduces parent's plant height
Apparent with mid-parent heterosis, the middle advantage of other Dominant Dwarfing Source plant heights is in ± 5%, and Fl is for plant height in mid-parent or so.
In conventional breeding, after Dominant Dwarfing Source homozygosis, plant is shorter (55cm or so), it is difficult to directly select containing the normal of indication law
Advise kind.It is 200510002391.1,200510053484.7 to have number of patent application, is connected using Rht10 genes and Ms2 genes
Lock, the method for establishing a set of dwarf male sterile wheat selection and breeding new varieties cultivate polling 981, polling 936, polling 987, polling 988 etc.
A series of wheat breeds.Number of patent application：201510140236.X using dominant dwarf Rht3 genes and ms1b gene linkages,
The method for establishing a set of selection and breeding conventional wheat new varieties.
Since Dominant Dwarfing Source is shorter, strain grain weight is reduced, in different genetic background, with Dominant Dwarfing Source plant height
It increases, yield also increases, and when the plant height of Dominant Dwarfing Source reaches 70cm or more, strain grain weighs to the weight of normal kind.In order to
Give full play to that wheat is dominant or the short effect of drop of semidominant dwarf gene, using the hybrid vigour of plant, can directly by dominant or
Semidominant wheat dwarf stem gene is used for crossbreeding.In China and foreign countries, scientist establishes a series of three systems, two systems and caryogram hybridization
In wheat breeding system, (mslb) recessive gene is utilized to establish a kind of using blue grain as the two line method hybrid wheat of mark property
Selection (number of applying for a patent, 200610042629.8) realizes genic male sterile in hybrid wheat production
Application, by dominant or semidominant wheat dwarf stem gene transformation to using blue grain as the selection and breeding of the two line method hybrid wheat of mark property
In method, realize that the breeding wheat for semidwarfness of hybrid wheat, application prospect are boundless.
A kind of " method of seed selection for new variety wheat " of patent of invention, the number of applying for a patent CN200510053484.7.It will be small
Wheat Dominant Dwarfing Source Rht10 genes are applied to conventional wheat breeding.Number of patent application：201510140236.X utilizing dominant dwarf
Rht3 genes and ms1b gene linkages, the method for establishing a set of selection and breeding conventional wheat new varieties.The deficiency of this method：This method
By indication law using be as a kind of application tool, the breeding method of foundation, not directly apply dominant dwarf not
Educate gene.A kind of method (application 201410177967.7) of rapid transfer wheat indigo plant mark sterile line and high-volume breeding, quickly
Transfer method.But without reference to the selection and breeding of the sterile line containing indication law.
Invention content
The present invention provides a kind of utilization method of Rht3 indication laws in hybrid vigour, it is therefore an objective to reduce hybridization
The plant height of wheat improves hybrid wheat kind ability resistant to lodging, reduces the risk of lodging, improves wheat yield.
Nuclear male sterility：The character of caused by nucleus male (stamen) not no viability.Wheat is recessive thin
Karyon male sterility gene mslb is located on the galianconism of wheat 4B chromosomes；During wheat indication law Rht3 is derived from
The thumb wheat in state Tibet, is similarly positioned on the galianconism of wheat 4B chromosomes, with the high stalk strain containing ms1b genes and contains
There is the wheat breed of the strain of short stem of Rht3 genes to be hybridized, obtains the middle stalk wheat F1 generation containing ms1b and Rht3, F1 generation
Selfing allows Chromosome recombination to exchange, and together by 2 gene linkages, cultivates containing chain small of mslb and Rht3 genetic recombination
Wheat sterile line of short stem.Wheat sterile strain of short stem is hybridized with blue mark type dual purpose lines, is returned, stable contain is selected
The chain excellent blue grain dual purpose lines (plant height 50cm or so) of short stem of mslb and Rht3, directly by the wheat product of the dwarf gene containing Rht3
System is applied to wheat heterosis and utilizes breeding.
Utilization method of the Rht3 indication laws provided by the invention in hybrid vigour,
Specifically, the method for technical solution includes the following steps：
1) it is maternal dual-purpose with ethereal blue grain indigo plant mark type containing chain Rht3-mslb gene dwarf wheat strains to cultivate
System is that male parent is hybridized, is returned, and selects the stable excellent blue grain dual purpose lines chain containing mslb-Rht3 and ethereal blue grain two
With being.
2) to be the maternal new varieties good with local general performance containing ethereal blue grain dual purpose lines chain mslb-Rht3 or new
Strain is that male parent is hybridized, and obtains the F1 generation of each male parent.F1 generation indigo plant grain is selected, the blue grain two new for isolation and selection next year
With being strain.
3) male parent (can also make female parent) is made with F1 generation, with the original kind (being) of screening be maternal (male parent can also be made) into
Row backcrossing obtains backcrossing F1BC1 for seed, selects and remain blue grain seed thin planting point plantation and occur in the group plant of plantation next year
The half short plant of 50% high stalk plant (plant height of original kind (being)) 50%.Select the short plant of excellent half (close to original kind
The character of (being)) it is selfed for selecting to stablize blue grain dual purpose lines of short stem.
4) it with selecting half excellent short plant (close to the character of original kind (being)) to continue to be returned with original kind (being), selects and remain
There is the 50% high stalk plant (strain of original kind (being) in the group plant of plantation next year in blue grain seed thin planting point plantation
It is high) 50% half short plant, select the short plant selfing of excellent half for select stablize indigo plant grain dual purpose lines of short stem.
5) it is obtained, with selecting half excellent short plant to continue to be returned with original kind (being), it is dilute selecting and remain blue grain seed with step 4)
Point plantation is planted, the short plant selfing of excellent half is selected, until selecting and excellent indigo plant of short stem similar in the character of original kind (being)
Grain dual purpose lines.
6) due to Rht3-mslb genes it is chain be comparison closely (cross-over value 6%), in step 3), 4), 5) in choosing
That selects contains sterile gene of short stem, and blue white race separates field plot test and is selfed to obtain wheat blue grain dual purpose lines of short stem.
The application method of the breeding wheat blue grain dual purpose lines of short stem of above method selection and breeding：White grain is used for crossbreeding, Lan Li
For breeding male sterile lines.
In order to achieve better technical results, above step 5) in, the backcrossing is continuous backcross 2-4 times.
In order to achieve better technical results, in above step (3)-(6), excellent half short plant proportion is
90%-98%.
In order to achieve better technical results, in above step (3)-(6), excellent half short plant proportion is 94%
Left and right.
Description of the drawings
The Breeding Process of Fig. 1 indigo plant grain dual purpose lines new product germlines of dwarf wheat containing Rht3-ms1b；
Specific implementation mode
Embodiment below is the further explanation to the present invention, but the present invention is not limited to following embodiments.
Embodiment 1：The selection and breeding of indigo plant grain dual purpose lines of short stem
Illustrate for the selection and breeding of 15N801 series blue grain dual purpose lines of short stem：
15N801 series is with river 07005 for parent sire of hybrid pigs, is female parent to contain Rht3-mslb gene dwarf wheat strains
Hybridized, the blue grain dual purpose lines of short stem of backcross breeding.
Spring in 2011 select it is established containing Rht3-mslb genes sterile wheat dual-purpose series 12CQ0026 of short stem,
The emasculation when wheat head of 12CQ0026 is released is pollinated with the pollen in river 07005, is selected blue grain Kunming summer numerous added-generation to plant F1, is gone
The backcrossing kind (F1BC1 generations) in river 07005 is obtained with the backcrossing of river 07005 after hero, blue grain seed (accounting for about 20%) is selected to be used for next year
Plantation, while Kunming summer numerous added-generation plantation F1 generation seed is harvested, select blue grain seed (accounting for about 34%) to be planted for next year；
Plant the blue grain seed of the backcrossing (F1BC1 generations) in Kunming summer numerous added-generation F1 and with river 07005 2011-2012 years；
In the F2 generations in spring in 2012, select excellent stalk plant of short stem or middle, and excellent middle stalk strain selfing is selected in F1BC1 generations, is selected light blue
Grain plantation (accounting for about 30%), by systematic breeding method selection and breeding to stable dual purpose lines of short stem.It spring in 2012, is selected in F2 is for group
Excellent middle stalk strain or short strain are made male parent and are returned with river 07005, and backcrossing seed (F2BC1 generations) is obtained, and blue grain seed is selected (to account for about
20%) it, is used to plant next year；Excellent middle stalk strain is selected in F1BC1 generations simultaneously, continues backcrossing (F1BC2 with river 07005
Generation), select blue grain seed (accounting for about 20%) to be planted for next year.The plantation of 2012 Kunming summer numerous added-generations is selected from F1BC2 and F2BC1 generations
The blue grain dual purpose lines seed selected is selected excellent middle stalk strain to make male parent in group and is returned with river 07005 is continued, and backcrossing seed is obtained
In F1BC3 and F2BC2 generations, select blue grain seed (accounting for about 20%), are planted for next year；The excellent middle stalk strain (plant height of simultaneous selection
75cm or so) selfing, excellent strain of short stem is selected in segregating population, by systematic breeding method selection and breeding to stable blue grain two of short stem
With being.Plant the excellent middle stalk selfed seed selected from Kunming summer numerous added-generation 2012-2013 years, group's plant height occurs high
Stalk, middle stalk and separation of short stem select excellent strain of short stem, by systematic breeding method selection and breeding to stable blue grain dual purpose lines of short stem；Selection
Excellent middle stalk can continue separate out excellent strain of short stem, select excellent blue grain dual purpose lines more than 50 of short stem altogether.
Note：07005 cultivar origin of river：It is crop institute of academy of agricultural sciences of Sichuan Province, is formed through seven generations selection and breeding in 5 years.Feature：
The Chongqing time of infertility is 185 days average, and spring habit, seedling half is upright, and tillering ability is strong, and Miao Ye is narrow, and growing way is vigorous.90 centimetres of plant height, fringe
Long 10.8cm, spikelet number 21.3, plant is neat, and strain blade is long.It is that a high Rust resistance, powdery mildew and stability are good, suitable
Ying Xingguang, red grain, best in quality, the breakthrough new variety of wheat of the good high yield of Comprehensive Traits.
The feature of 15N801 series blue grain dual purpose lines of short stem：Spring habit, seedling is upright, and tillering ability is stronger, dark green leaf color,
Miao Ye is narrower.Average plant height 52.6cm, spike length 10.3cm, spikelet number 19.8, plant type is compact, and fringe layer is neat, and fringe is closeer, rectangular
Shape fringe, long awns, red grain, fertilizer resistance anti-lodging.
The white grain sterile lines seed making of the blue grain dual purpose lines of short stem of embodiment 2 and F1 generation performance
2014-2015 selects the white grain sterile lines seed making of excellent blue grain dual purpose lines of short stem in Chongqing, selects 44 infertility
System's restorer (male parent) production of hybrid seeds different from 12 carries out artificial supplementary pollination spring in 2015, harvests cross combination 392 altogether
A, in these combinations, mu spike number has 8 250,000 or more, has 52 in the combination of 200,000 fringes；Mass of 1000 kernel is in 45g or more groups
2 are closed, there are 66 in the combination of 40g or more, combination 1 of the grain number per spike at 45 or more, in 40 or more combinations 9, system
Combination 2 of the kind yield at 300kg/ mus or more, combination 13 of the hybrid seed yield at 250kg/ mus or more, High yield combination production of hybrid seeds production
It is horizontal that amount reaches Chongqing locality Wheat Production.Solid relatively good cross combination 367 is selected from 392 combinations, in 2015-
2016 years carried out variety comparative test plantation, the feature of F1 generation cenospecies：Spring habit, seedling is upright, and tillering ability is stronger, and leaf color is deep
Green, Miao Ye is narrower.Plant height 65-75cm, plant type is compact, fertilizer resistance anti-lodging.Fringe layer is neat, spike length 10-12cm, rectangle, spikelet number
20-24, grain number per spike 37-48.Maturity period is suitable with Chongqing region commercial variety.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (5)
- Utilization method of the 1.Rht3 indication laws in hybrid vigour, it is characterised in that：Include the following steps：1) it is that female parent is with ethereal blue grain indigo plant mark type dual purpose lines containing chain Rht3-mslb gene dwarf wheat strains to cultivate Male parent is hybridized, is returned, and selects the stable excellent blue grain dual purpose lines chain containing mslb-Rht3 and ethereal blue grain is dual-purpose System；2) to be the maternal new varieties or new lines good with local general performance containing ethereal blue grain dual purpose lines chain mslb-Rht3 Hybridized for male parent, obtains the F1 generation of each male parent；F1 generation indigo plant grain is selected, the blue grain dual purpose lines new for isolation and selection next year Strain；3) male parent (can also make female parent) is made with F1 generation, is that maternal (can also make male parent) is returned with the original kind (being) of screening It hands over, obtains backcrossing F1BC1 for seed, select and remain blue grain seed thin planting point plantation and occur 50% in the group plant of plantation next year High stalk plant (plant height of original kind (being)) 50% half short plant；Select the short plant of excellent half (close to original kind (being) Character) selfing stablizes blue grain dual purpose lines of short stem for selecting；4) with selecting half excellent short plant (close to the character of original kind (being)) to continue to be returned with original kind (being), blue grain is selected and remain Seed thin planting point is planted, and in the group plant of plantation next year, 50% high stalk plant (plant height of original kind (being)) occurs 50% half short plant, select the short plant selfing of excellent half for select stablize indigo plant grain dual purpose lines of short stem；5) it is obtained, with selecting half excellent short plant to continue to be returned with original kind (being), selecting and remain blue grain seed thin planting point with step 4) Plantation, selects the short plant selfing of excellent half, until selecting and excellent blue grain two of short stem similar in the character of original kind (being) With being；6) due to Rht3-mslb genes it is chain be comparison closely (cross-over value be 6% or so), in step 3), 4), 5) in choosing That selects contains sterile gene of short stem, and blue white race separates field plot test and is selfed to obtain wheat blue grain dual purpose lines of short stem.
- 2. utilization method of the Rht3 indication laws according to claim 1 in hybrid vigour, it is characterised in that：With In upper step 5), the backcrossing is continuous backcross 2-4 times.
- 3. utilization method of the Rht3 indication laws according to claim 1 in hybrid vigour, it is characterised in that：With In upper step (3)-(6), excellent half short plant proportion is 90%-98%.
- 4. utilization method of the Rht3 indication laws according to claim 3 in hybrid vigour, it is characterised in that：With In upper step (3)-(6), excellent half short plant proportion is 94%.
- 5. selection and breeding according to claim 1, the application method of breeding wheat blue grain dual purpose lines of short stem, it is characterised in that：In vain Grain is used for hybrid seeding and cenospecies breeding, and blue grain is used for breeding male sterile lines.
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| CN201810230178.3A CN108496791A (en) | 2018-03-20 | 2018-03-20 | Utilization method of the Rht3 indication laws in hybrid vigour |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113557957A (en) * | 2021-07-28 | 2021-10-29 | 西南大学 | Breeding method and application of blue-standard two-line hybrid wheat system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826875A (en) * | 2006-04-06 | 2006-09-06 | 西北农林科技大学 | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character |
| CN104719129A (en) * | 2015-03-27 | 2015-06-24 | 西南大学 | Breeding method of novel wheat variety |
-
2018
- 2018-03-20 CN CN201810230178.3A patent/CN108496791A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826875A (en) * | 2006-04-06 | 2006-09-06 | 西北农林科技大学 | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character |
| CN104719129A (en) * | 2015-03-27 | 2015-06-24 | 西南大学 | Breeding method of novel wheat variety |
Non-Patent Citations (2)
| Title |
|---|
| TOBIAS WÜRSCHUM ET.AL.,: "Exploiting the Rht portfolio for hybrid wheat breeding", 《THEORETICAL AND APPLIED GENETICS》 * |
| 行翠平等: "小麦蓝粒两用系杂交后代遗传分离规律研究", 《陕西农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113557957A (en) * | 2021-07-28 | 2021-10-29 | 西南大学 | Breeding method and application of blue-standard two-line hybrid wheat system |
| WO2023005883A1 (en) * | 2021-07-28 | 2023-02-02 | 西南大学 | Breeding method of blue marked two-line hybrid wheat system and use |
| CN113557957B (en) * | 2021-07-28 | 2023-03-14 | 西南大学 | Breeding method and application of blue-standard two-line hybrid wheat system |
| GB2624547A (en) * | 2021-07-28 | 2024-05-22 | Univ Southwest | Breeding method of blue marked two-line hybrid wheat system and use |
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