CN105340730B - The selection of wheat indigo plant grain near isogenic lines - Google Patents
The selection of wheat indigo plant grain near isogenic lines Download PDFInfo
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Abstract
The invention discloses a kind of selections of wheat indigo plant grain near isogenic lines, belong to field of wheat breeding.The selection and breeding of indigo plant grain near isogenic lines of the invention be by the blue grain of linkage inheritance, of short stem, three character passability shapes of Anther Abortion exchange, break blue grain and dwarfing male sterile, the linkage relationship of two character genes of short stem, and obtain plant height it is normal, can self-fertility blue grain germplasm.For the present invention by the blue short backcross population selection and breeding indigo plant grain near isogenic lines for losing homozygosis and stablizing, probability is big, speed is fast, simple and easy to do.It can implement the big kind pursuit of blue grain kind whereby with surmounting strategy by any one kind transformation into blue grain system-indigo plant grain near isogenic lines of the kind with the present invention.
Description
Technical field
The present invention relates to field of wheat breeding, more particularly to a kind of selection of wheat indigo plant grain near isogenic lines.
Background technology
With the raising of living standard, it is desirable to eat good health and a long life, be pursuit of the people to food of new generation.Big
While good health and a long life are pursued by family, the rich people's diseases such as all kinds of cardiovascular and cerebrovascular diseases and obesity, diabetes are more and more, it is desirable to
Huge contrast is formd with reality, causes the anxiety that people are universal, how to reach health care and diseases prevention by diet becomes
The hot spot paid close attention to jointly.
Colorful wheat（Include the wheat of the colors such as blue, green, purple）With excellent nutritional quality and higher anti-
Sick health value, is increasingly pursued by consumers and market, has more already been caused the concern of wheat breeding person and has been ground
Study carefully interest.
What Accessories during Binzhou crop research institute Geng Aimin researcher presided over《Colorful wheat breeding of new variety is studied》Project is put into
Shandong Province's development in science and technology plan（Number 2008GG3NS08001）.Blue-grain wheat is studied according to it in endosperm and aleurone to contain
A large amount of have effects that the anthocyanidin of health care diseases prevention, and to elements such as zinc, iron, selenium with enrichment, these plant source nutrition into
It part is easier to than strengthening the nutrient of addition as absorption of human body, and safe and reliable.And the wheat tool of other colors of blue-grain wheat
There is better palatability, be comparatively ideal biological reinforced variety type.
" blue short loses " is that blue grain, of short stem, three individual character of Anther Abortion turn the special wheat germplasm of linkage inheritance, we observe hair
Often occur that plant height is normal, is capable of one kind " indigo plant educates strain " of normal self-fertility in " blue short loses " group now（It can be bred as blue grain
The type of kind）, and its relative scale is higher（Between one to ten percent, average probability is reached in percentage its occurrence probability
Five or so）.Accordingly, we by the approach, can be bred as the blue grain system of " blue short loses " backcross --- and i.e. blue grain is closely etc.
Gene line, and blue-grain wheat Production of Large Fields is used it for, it is provided for people with health protection and disease prevention function blue-grain wheat food, service
In compatriots' health.
Due to being limited by " indigo plant is short to be lost " germ plasm resource, also no one utilizes the approach of the present invention and technology selection and breeding indigo plant grain so far
Near isogenic lines.
Invention content
In order to make up the blank of the prior art, the present invention provides a kind of selection of wheat indigo plant grain near isogenic lines,
Wheat indigo plant grain Developing Near Isogenic Lines probability of the present invention is big, and speed is fast, has good foresight, repeatability.
The technical scheme is that：
A kind of wheat indigo plant grain near isogenic lines, for the blue short blue grain for losing linkage inheritance, of short stem, three characters of Anther Abortion,
It exchanged by the way that character occurs, break blue kernel Characters and dwarfing male sterile, the linkage relationship of two character genes of short stem, and obtain energy
The blue grain germplasm of normal self-fertility.
The selection of the wheat indigo plant grain near isogenic lines, including step：
1）It is blue it is short lose in backcross population the fertile plant that selection plant height is normal, blossoms and bears fruit, the fertile plant is covered
Bag processing removes bagging after roguing self-fertility, and removes the whereabouts tillering tassel for loose powder of not yet blooming；
2）Sort the blue grain seed plantation in the fertile plant, it is ripe before according to the general performance roguing of plant, select fringe, and
To roguing, select fringe threshing and species test；Retain the roguing of all blue seeds, select fringe, eliminate choosing of the blue white dichromatism seed with fringe
Fringe is selected in strain；
3）By the complete blue grain-by-grain seed selection strain selected and remain, fringe kind is selected to carry out plant, head progeny row field test into plant, head progeny row, screen blue grain
Near isogenic lines.
Preferably, the backcross population is the blue short backcross population for losing homozygosis and stablizing.
Preferably, step 1）In, bagging processing specifically uses the whole strain bagging of sheepskin paper bag.
Preferably, step 3）Described in blue grain near isogenic lines show as in addition to grain color is blue grain, remaining is other
Whole characters are identical with the backcrossing male parent varieties of " indigo plant is short to be lost ".
The selection and breeding of indigo plant grain near isogenic lines of the invention are to lead to the blue grain of linkage inheritance, of short stem, three characters of Anther Abortion
Character is crossed to exchange, break blue grain and dwarfing male sterile and the linkage relationship of two character genes of short stem, and acquisition can normally certainly
The blue grain germplasm of knot reality.
With selection and breeding indigo plant grain near isogenic lines of the present invention, any one kind transformation can be gone out to the blue grain of the kind
It is --- --- blue grain near isogenic lines, the big kind pursuit of blue grain kind can be implemented whereby with surmounting strategy.
In the Lan Aibai homozygous backcross group of plantation, the plant height for selecting expectable appearance is normal（BELAND is short to lose height）, have
Anther can the indigo plant of normal self-fertility educate strain, be listed to these plant of discovery, and with the whole strain bagging of parchment sack, after solid
Bagging is removed, singly receives after ripe and singly puts, species test threshing is tied blue white dichromatism seed with the wheat head to the type plant and sorted.
These blue grain seeds for sorting out of simple grain program request, and fringe is selected in roguing from group, those colors are eliminated in species test threshing
It is not homozygous（There are still blue white grains）Roguing or select fringe, to complete blue grain-by-grain seed selection strain therein and select fringe.During autumn sowing, plant into plant with
Head progeny row, and plant the blue short backcrossing male parent varieties lost and compare, is recorded by field investigation, and observation is compared, select and remain it is overall with it is right
According to appearance appearance, the plant and head progeny row that feature is identical, general performance is good, be listed number, single single threshing of receipts, poidometer production,
And indoor species test is carried out, select and remain that yield is high, variable rate technology is good, full seed, blue are compared with depth and the consistent plant or head progeny row of the depth,
It is mixed into the blue short blue grain near isogenic lines for losing backcrossing male parent varieties.Plant with head progeny row yield can be also higher than and compareed, performance is excellent
Different material carries out cell plantation observation and Yield comparison, and group's performance of material is respectively selected and remain under the conditions of Production of Large Fields.
By comparing, the blue grain near isogenic lines that those characters are stable, Comprehensive Traits are good, yield is high is selected and remain, realizes blue grain kind（Or product
System）The synthesis of original kind is surmounted.
The blue grain near isogenic lines of those big kinds, these blue grain kinds rich in anthocyanidin, Fe-Zn enriched selenium, as day
Normal health food and beverage main material will be paid attention to by the producer and consumer welcomes.The invention is significant.
Blue short lose is blue grain, special wheat germplasm of short stem, three character linkage inheritances of Anther Abortion, in Lan Aibai groups
In often occur that plant height is normal, is capable of a kind of indigo plant of normal self-fertility educates strain（It can be bred as the type of blue grain kind）, and its
Relative scale is higher（Between one to ten percent, average probability is reached 5 percent or so its occurrence probability）.It may be control
The different position gene of the blue grain of system（4D/4E）, with the dwarf gene on known 4D the short arm of a chromosome S（Rht10）With Taigu core anther
Abortion gene（Ms2）It is distant, it is likely that Lan Li（4D/4E）Different position gene is easier to break company on 4D chromosome long arms L
Lock occurs character and exchanges.
Beneficial effects of the present invention are：
1st, the present invention loses the homozygous backcross population selection and breeding indigo plant grain near isogenic lines stablized by the way that indigo plant is short, probability is big, speed is fast,
It is simple and easy to do.
2nd, with the present invention can easily and the acquisition lifted any one stablize " blue short loses " backcross population it be returned male parent
The blue grain near isogenic lines of kind.And it can directly use it for the Production of Large Fields of blue-grain wheat.
3rd, the big kind that can implement blue grain kind with the present invention is pursued with surmounting.It such as can be easily using the present invention
Obtain blue grain near isogenic lines-indigo plant grain Jimai 22 of Jimai 22, it might even be possible to surmount Jimai 22 in yield and resistance etc.（It is former
Backcross）Blue grain Jimai 22.
Specific embodiment
Embodiment 1
The selection and breeding of the blue near isogenic lines in Mount Taishan 23.
A. sowing indigo plant is short loses 23 homozygous backcross group of Mount Taishan.
In the autumn in 2011 by the short seed for losing 23 homozygous backcross group of Mount Taishan of indigo plant of the current year summer harvest, the blue white grain of sorting will divide
The blue grain seed sorted out uses the motor-driven cell of 6 rows/head progeny row planting with sowing machine, 20 centimetres of line-spacing, and cell row grows 5 meters, and indigo plant is short to be lost back
Hand over population size about 1000, it is blue it is short lose both sides after emergence and respectively sow a line Mount Taishan 23 do backcrossing male parent, male parent uses 10
Centimetre wide seedling band artificial broadcast, away from it is blue it is short lose 25 centimetres, overwintering to it is blue it is short lose covering with ground sheeting, the crop field wheat of spring in 2012 rises
The body phase（Blue short lose about pulls out 2 for sections in film）Film is taken off, takes off after film fertilizing, watering immediately, early April is with duck's egg bamboo, iron wire, plastic knitting
Cloth builds divider wall, and divider wall is about 1.2 meters high, establishes isolated area.
B. the indigo plant that selection plant height is normal, has anther educates strain.
In late April, 2012 short lose of indigo plant is eared successively, notices that the plant height occurred in observation Lan Aibai groups is normal, has anther
Plant, be listed to such roguing of discovery number, registration related information, and in time with big parchment bag（Long 80cm wide
20cm）Whole strain bagging, with the Lan Aibai groups near pollen-proof diffusion pollution.Remove bagging in time after the roguing self-fertility,
And remove the whereabouts tillering tassel for loose powder of not yet blooming.Choose 28 roguings altogether in the group.Strain species test threshing is pressed after harvest.Its
Roguing is sorted with fringe knot indigo plant, white dichromatism seed to bearing seeds by grain color.
C. it selects complete blue grain-by-grain seed selection strain or selects fringe
Autumn sowing in 2012, to the blue grain kind that above-mentioned reservation roguing sorts out into plant selective nursery, from 28 roguings, preferentially
24 roguings have been planted, have used strain/head progeny row seeder, 1 single plant of often row sowing, often 6 single plants of section row, common row are 4 sections, often
Section row is 4 meters long.Routinely crop field fertilizer and water management according to the general performance roguing of plant and selects fringe before ripe, to roguing and selects fringe
Species test threshing.It sends out roguing existing by threshing, select all blue seeds of fringe, some blue, white dichromatism seed still occurs, eliminates
Fringe is selected in the roguing of whole grain color separations.Retain complete blue choosing with selecting fringe.
D. the blue grain near isogenic lines in selection and breeding Mount Taishan 23.
Autumn sowing in 2013 by the complete blue grain-by-grain seed selection strain selected and remain and selects fringe according to line breeding program, and whole kinds into plant and fringe
Row.It plants Mount Taishan 23 simultaneously to compare, carries out plant and head progeny row field test.According to experimental field being considered with workload, we
Optimum selecting remains 10 strains from 24 strains, and each strain plants 6 head progeny rows（1 section）, plant and saved for 10, from remaining strain
12 are preferentially selected in system and selects fringe, 2 section of plantation, 1 section Mount Taishan 23 of sowing is control, altogether 13 section, and often section row is 2 meters long.Section and section
Between stay 0.5 meter of small aisle.
Farm record is established, each period carries out investigation record, compares plant type, the fringe of each plant of complete blue grain strain and head progeny row
Variable rate technology in terms of type, plant height, growing way, appearance, disease resistance, resistance, ripe equal feature of ripe phase, observation are compared and Thailand
The similarities and differences between mountain 23.It eliminates appearance and grows the big plant and head progeny row of equal feature difference, retain identical with 23 appearance of Mount Taishan
Plant and head progeny row, by plant, head progeny row, individually harvesting line by line, threshing respectively, poidometer production, and each head progeny row yield is added up into system
Count out the yield of each strain.Indoor species test is carried out one by one to the seed of each head progeny row, it is of light color to eliminate those hollow kernelss, blue grain
Or the plant and head progeny row of deep mixed cause.
These appearance appearance and feature are all identical, and in addition to seed is different from Mount Taishan 23 for blue, remaining character is complete
Portion is identical and the blue grain near isogenic lines of homozygous consistent plant and head progeny row material, referred to as Mount Taishan 23.
The seed of select and remain 66 strains of section is mixed into the blue near isogenic lines in Mount Taishan 23.These seeds are in kind of a grain
Rich and influential family, home farm's plantation produce for the order of blue-grain wheat.
E. the blue near isogenic lines brief introduction in Mount Taishan 23：The indigo plant grain near isogenic lines is by Mount Taishan 23 " indigo plant is short to be lost " homozygous backcross group
Body exchanges selection and breeding by character and forms.Semi-winterness, seedling half are upright.Tillering ability is strong, and the percentage of earbearing tiller is high, per acre 43.4 ten thousand fringe of mu；, it is ripe
Mutually preferably；75.2 centimetres of plant height, grain number per spike 32.8,45.5 grams of mass of 1000 kernel；Plant type is compact, is rushed on blade, more resistant to lodging；Fringe layer
Neatly, spindle fringe, long awns, white shell indigo plant grain, seed is fuller, semi-rigid, muscle veriety in category.The ripe phase is identical with Mount Taishan 23, ripe
Mutually general, stripe rust resisting feels powdery mildew and banded sclerotial blight.
F. blue grain kind surmounts implementation action.
Autumn sowing in 2014 shows most excellent 6 head progeny rows to last year, carries out cell production comparison, 6 row areas, row long 6
Rice, 7.2 square metres of plot area are check variety with Mount Taishan 23, are repeated 2 times.Conventional field management compares in Production of Large Fields item
The performances such as the yield under part.23 indigo plant of Mount Taishan is No. 1 fringe system highest volume increase 4.8%, test statistics result and the volume variance in Mount Taishan 23
Not significantly.The big of Mount Taishan 23 is surmounted though failing to realize, the blue grain as Mount Taishan 23 dominate tie up to it is sharp during order produces
With well received and accreditation.
Embodiment 2
Step on the selection and breeding of the blue near isogenic lines in sea 5197.
Links are completely identical with case one, and this will not be repeated here.But the blue grain near isogene for stepping on sea 5197 ties up to
Far away from the blue grain near isogenic lines in Mount Taishan 23, main cause is to step on sea 5197 and 23 two, Mount Taishan kind sheet for application in production
Caused by body yield and adaptability difference.
It follows that want selection and breeding indigo plant grain near isogenic lines will from those big kind transformations into " blue short loses " backcross population in
Otherwise selection and breeding, though blue grain near isogenic lines can be selected, may not necessarily obtain substantive application.
Claims (1)
1. the selection of wheat indigo plant grain near isogenic lines, which is characterized in that including step：
A. sowing indigo plant is short loses 23 homozygous backcross group of Mount Taishan；
In 1 year autumn by the short seed for losing 23 homozygous backcross group of Mount Taishan of indigo plant of the current year summer harvest, the blue white grain of sorting will sort out
Blue grain seed using the motor-driven cell of 6 rows/head progeny row planting with sowing machine, 20 centimetres of line-spacing, cell row grows 5 meters, blue short to lose backcrossing group
Body scale has 1000, it is blue it is short lose both sides after emergence and respectively sow a line Mount Taishan 23 do backcrossing male parent, male parent uses 10 centimetres of wide seedlings
Band artificial broadcast, away from it is blue it is short lose 25 centimetres, overwintering to it is blue it is short lose covering with ground sheeting, next year spring crop field wheat is stood up in phase film
Blue short lose pulls out 2 for sections and takes off film, takes off after film that fertilizing, watering, early April build divider wall with duck's egg bamboo, iron wire, plastic woven cloth immediately,
Divider wall is 1.2 meters high, establishes isolated area；
B. the indigo plant that selection plant height is normal, has anther educates strain；
Late April short lose of indigo plant is eared successively, and the plant height occurred in observation Lan Aibai groups is normal, has the plant of anther, to finding
Such roguing be listed number, registration related information, and with big parchment bag, long 80cm wide 20cm, whole strain bagging, to prevent
Lan Aibai groups near pollen diffusion pollution；Remove bagging after the roguing self-fertility, and remove and not yet bloom under loose powder
Fall tillering tassel；Choose 28 roguings altogether in the group；Strain species test threshing is pressed after harvest；Its roguing is the same as fringe knot indigo plant, white dichromatism seed
Grain, is sorted to bearing seeds by grain color；
C. it selects complete blue grain-by-grain seed selection strain or selects fringe
Current year autumn sowing to the blue grain kind that above-mentioned reservation roguing sorts out into plant selective nursery, from 28 roguings, is preferentially planted
24 roguings have used strain/head progeny row seeder, 1 single plant of often row sowing, often 6 single plants of section row, and row is 4 sections altogether, often section row length
4 meters；Routinely crop field fertilizer and water management according to the general performance roguing of plant and selects fringe before ripe, to roguing and fringe species test is selected to take off
Grain；It sends out roguing existing by threshing, select all blue seeds of fringe, some blue, white dichromatism seed still occurs, eliminates whole grains
Fringe is selected in the roguing of color separation；Retain complete blue choosing with selecting fringe；
D. the blue grain near isogenic lines in selection and breeding Mount Taishan 23；
Next year autumn sowing by the complete blue grain-by-grain seed selection strain selected and remain and selects fringe according to line breeding program, and whole kinds into plant and head progeny row；Together
When plantation Mount Taishan 23 compare, carry out plant and head progeny row field test；According to experimental field being considered with workload, from 24 strains
Middle optimum selecting remains 10 strains, and each strain plants 6 head progeny rows, and 1 section is planted and saved for 10, preferentially selected from remaining strain
Go out 12 and select fringe, 2 section of plantation, 1 section Mount Taishan 23 of sowing is control, altogether 13 section, and often section row is 2 meters long；0.5 meter is stayed between section and section
Small aisle；
Farm record is established, each period carries out investigation record, relatively the plant type of each plant of blue grain strain and head progeny row, fringe type, strain entirely
Variable rate technology in terms of height, growing way, appearance, disease resistance, resistance, ripe phase character characteristic of ripe phase, observation compare with Mount Taishan 23 it
Between the similarities and differences；Eliminate appearance appearance feature difference big plant and head progeny row, retain the plant identical with 23 appearance of Mount Taishan and
Each head progeny row yield aggregate statistics by plant, head progeny row, line by line individually harvesting, respectively threshing, poidometer production, and are gone out each strain by head progeny row
The yield of system；Indoor species test is carried out one by one to the seed of each head progeny row, eliminate that those hollow kernelss, blue grain be of light color or the depth not
Consistent plant and head progeny row；
These appearance appearance and feature are all identical, in addition to seed is different from Mount Taishan 23 for blue, remaining character whole phase
The blue grain near isogenic lines of same and homozygous consistent plant and head progeny row material, referred to as Mount Taishan 23.
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| JP2019129782A (en) * | 2018-02-01 | 2019-08-08 | 株式会社水稲生産技術研究所 | Rice and wheat having desired texture, and production method thereof |
| CN111972277B (en) * | 2020-08-11 | 2022-07-26 | 淄博爱民种业有限公司 | Method for breeding black wheat germplasm with different genetic backgrounds by utilizing blue dwarf male sterile and purple wheat |
| CN111972279B (en) * | 2020-08-11 | 2022-09-27 | 淄博爱民种业有限公司 | Breeding method for obtaining blue dwarf male sterile germplasm with different blue grain fertile plant exchange rates |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826875A (en) * | 2006-04-06 | 2006-09-06 | 西北农林科技大学 | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character |
| CN104719123A (en) * | 2015-02-12 | 2015-06-24 | 山东滨州黑马种业有限公司 | Method for creating winter dwarf male-sterile wheat with blue seeds |
-
2015
- 2015-11-25 CN CN201510827910.1A patent/CN105340730B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826875A (en) * | 2006-04-06 | 2006-09-06 | 西北农林科技大学 | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character |
| CN104719123A (en) * | 2015-02-12 | 2015-06-24 | 山东滨州黑马种业有限公司 | Method for creating winter dwarf male-sterile wheat with blue seeds |
Non-Patent Citations (1)
| Title |
|---|
| "蓝矮败"——小麦杂种优势利用的第五途径;耿爱民等;《山东农业科学》;20101231;31-33 * |
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