CN108812299A - Circulation modification method in corn Suwan germplasm group - Google Patents
Circulation modification method in corn Suwan germplasm group Download PDFInfo
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- CN108812299A CN108812299A CN201810776170.7A CN201810776170A CN108812299A CN 108812299 A CN108812299 A CN 108812299A CN 201810776170 A CN201810776170 A CN 201810776170A CN 108812299 A CN108812299 A CN 108812299A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses the circulation modification methods in a kind of corn Suwan germplasm group.After the present invention is by hybridizing using preferable Elite inbred in selection production or being returned constructional base group, basic population continuous multi-generation selfed breeding corn inbred line with the means of shuttle breeding from establishment is selected using improvement I and II Pedigree selection method, it has polymerize the excellent characteristics of Suwan corn germplasm, the self-mating system Q2232 that present inventor is bred as according to the above method is compared with QR273, QB48, the combination that T32 is assembled, increase production 8.6% respectively, 13.2%, 16.55%, illustrate that this method effectively raises yield combining ability, and it is resistance to lean to show drought resisting, it is disease-resistant, wide adaptability and plant type is good, breeding and the production application for improving Southwestern China mountain area are horizontal, the corn hybrid seed for adapting to Southwestern China mountain area for breeding is laid a good foundation.
Description
Technical field
Circulation modification method the present invention relates to agricultural technology field, in specially a kind of corn Suwan germplasm group.
Background technique
Corn is the important cereal crops in the world and the maximum cereal crops of China's cultivated area, in grain security
With important strategic position.
The geographical location in Guizhou is in In Southwest China center, and Kweiyang is in the middle part in Guizhou, and weather is special
Selecting is warm no heat of raw rice growth period, and soil fertility is medium, so the self-mating system selected herein and cenospecies can width
Penetrate surrounding area；Xingyi is in the west and south in Guizhou, this local heat abundance, weather Wen Liang, abundant rainfall, sunshine is sufficient, has
Conducive to disease generation, so the disease resistance in this selecting and breeding corn is fine.Therefore the corn breeding that Guizhou In China saves is with richly endowed by nature
Geography and climate advantage, be corn germplasm improvement innovation ideal place.
Suwan group is that the farm Thailand Su Wan is identified from the corn variety introduced all over the world to Thailand, filters out synthesis
Do very well, 36 parts of materials of rich hereditary variation formed, by improveing selection for a long time, now by whole world application, therefore,
The excellent genes of Suwan germplasm excavate endless.
In recent years, the kind overwhelming majority promoted in China's production contains Suwan blood relationship, and especially in southwest, breeding is miscellaneous
Handing over kind is prior germplasm materials.However the self-mating system plant type of the direct breeding of Suwan group is bad, the characters problem such as photosensitive compared with
It is more.
It was verified that its advantage respectively is excavated, so that reaching anti-using preferable Elite inbred is applied in production
Resistance to lean, the disease-resistant, wide adaptability of drought, the breeding goal that coordinate force is high and plant type is good；Therefore, inventor seminar is selected from corn
After the Elite inbred T32 of Suwan group hybridizes with QB48, breeding at stabilization self-mating system QB572, QB576 it is miscellaneous with QR273 again
The basic population set up is handed over or be returned, by improvement I and II Pedigree selection method and shuttle breeding means, is bred as a batch with height
Match, is mostly anti-, the Elite inbred that wide suitable, high yield and plant type are good, such as QB2232, QB1923, QB2208；It is new corn has been authorized
Kind 3 (Zhuo Yu 2：National level authorization, gold and jade 579：Guizhou, the authorization of two province of Yunnan, hundred grand beautiful 988：Guizhou authorization),
Participation area (pre-) Varieties 12.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides the circulation modification method in a kind of corn Suwan germplasm group, warps
Suwan germplasm group after crossing this method improvement is suitable for the good self-mating system of Breeding Traits, with overcome the deficiencies in the prior art.
In order to achieve the above object, the present invention is achieved by the following technical programs：Circulation in corn Suwan germplasm group
Modification method includes the following steps：
Step 1：Apply preferable Elite inbred T32 and QB48 miscellaneous using selected from corn Suwan group and in production
It hands over, by its F1After plantation, using improvement I Pedigree selection method selection, i.e. F1For mixed pollination, mixed planting, mixing threshing, do not select
It selects, F2Start individual plant selfing, head progeny row plantation and selection, the selfing of incubation and does combining ability test；
Step 2：The F that step 1 is obtained2After seed plantation, by the selection method of step 1), stringent antagonism and plant property
Shape is selected, and carries out fringe choosing when harvest, and continuous bag sleeving is from being sent to F6-8In generation, selects highest two self-mating systems of coordinate force；
Step 3：It, will after two self-mating systems in step 2 are hybridized or are returned with from the QR273 of Suwan1C11 breeding again
Its F1After plantation, using improvement II Pedigree selection method selection, i.e. F1Generation do not select, mixed pollination and be returned, mix threshing, mix
Close plantation, F2Or F1BC1In generation, starts individual plant selfing, head progeny row plantation and selection, F2In generation, starts to shuttle in strange land to plant selection later,
Stringent antagonism and tree characteristics are selected, and carry out fringe choosing when harvest, and continuous bag sleeving is from being sent to F5-6In generation, obtains the jade of improvement
Rice Suwan germplasm group.
Described in step 1 selection refer to by embodiment selection principle and method to different times antagonism,
The characters such as plant type, florescence harmony, fruit ear are strictly selected；Selection criteria described in step 3 is the same as step 2.
Beneficial effect
Compared with prior art, the present invention is set up by selecting to hybridize or be returned using preferable Elite inbred in production
After basic population, the basic population using improvement I and the selection of II Pedigree selection method and means from the establishment of shuttle breeding is continuously more
For selfed breeding corn inbred line, the excellent characteristics of Suwan corn germplasm has been polymerize it, present inventor is according to the above method
The self-mating system Q2232 of incubation increases production 8.6%, 13.2%, 16.55%, explanation compared with the combination that QR273, QB48, T32 are assembled respectively
This method effectively raises yield combining ability, and shows resistance to lean, the disease-resistant, wide adaptability of drought resisting and plant type is good, improves China
The breeding and production application of Southwest Mountainous Areas are horizontal；The difficulty for reducing the direct breeding of Suwan group, because Suwan population genetic becomes
It is different big, cause that there is also problems in the self-mating system directly selected, and the present invention is conducive to using " formula gradually " improvement
The continuous improvement with beneficial gene is constantly tamed in Long-term selection, its purpose is to provide in a kind of corn Suwan germplasm group
Recycle modification method.The corn hybrid seed for adapting to Southwestern China mountain area for breeding is laid a good foundation.
Detailed description of the invention
Attached drawing 1 is flow diagram of the invention.
Specific embodiment
Implementation steps of the invention：Circulation modification method in corn Suwan germplasm group, includes the following steps：
Step 1. applies preferable Elite inbred T32 and QB48 miscellaneous using selected from corn Suwan group and in production
It hands over, by its F1After plantation, using improvement I Pedigree selection method selection, i.e. F1For mixed pollination, mixed planting, mixing threshing, do not select
It selects, F2Start individual plant selfing, head progeny row plantation and selection, the selfing of incubation and does combining ability test.
The F that step 2. obtains step 12Seed plantation；Then, individual plant selfing is stringent right by the selection method of step 1
Resistance and tree characteristics are selected, and carry out fringe choosing when harvest, and continuous bag sleeving is from being sent to F6-8For when it is basicly stable, number is
QB569-QB578, through combining ability test, the coordinate force of QB572 and QB576 are higher.
The F2Start individual plant selfing, head progeny row plantation with select specific operation process for,
1) individual plant selfing：By F2200-300 plants are planted, is being opened after plantation by 3500-4000 plants/acre of density for seed
Florescence carries out the selfing of single plant bagging, single fringe threshing after harvest, and packing number obtains F3For seed；
2) head progeny row is planted：By selected F3Seed plantation is single after florescence carries out single plant bagging selfing harvest at head progeny row
Fringe threshing, packing number, obtains F4Seed, until stablizing；
3) planting density：From F3In generation, starts the density that every selection generation increases 100-200 plants/acre；
4) selection principle：F2For plant height 2.6m hereinafter, fringe position 1.4m is hereinafter, every selection generation is by reduction 5-8cm's later
Standard；Loose powder of spinning coordinates (single plant flower synchronization)；Comprehensive disease resistance resists the above, setting percentage to be up to 96% or more, seed in reaching
The fruit ear of Hard grain type is selected fruit ear；
5) selection method：In strict accordance with selection principle 4), in the plant that florescence coordinates the plant height Ear height florescence
Carry out the selfing of single plant bagging；Mainly the setting percentage of plant disease resistance, fruit ear, the commodity of seed are selected in full ripe stage；
6) harvest-seed extraction：Selected fruit ear is subjected to single fringe threshing, the kind after line number is write in pack, as next generation's plantation
Son.
After two self-mating systems in step 2 are hybridized or be returned with from the QR273 of Suwan1C11 breeding again by step 3., by it
F1After plantation, using improvement II Pedigree selection method selection, i.e. F1Generation do not select, mixed pollination and be returned, mix threshing, mix
Plantation, F2Or F1BC1In generation, starts individual plant selfing, head progeny row plantation and selection, the self-mating system of incubation and does combining ability test, final to be bred as
The available self-mating system of a batch.
The F1For mixed pollination and does backcrossing and refers to,
It pollinates after florescence takes male flower to mix to female fringe, and does 3-5 fruit ear of backcrossing with QR273, harvest fruit ear
Threshing is mixed respectively.
The F2Or F1BC1Generation start individual plant selfing, head progeny row plantation with select specific operation process for,
1) individual plant selfing：By F2Or F1BC1It is other to be same as above by 4000-4200 plants/acre of density for seed；
2) head progeny row plantation and selection：Ibid；
F2In generation, starts to shuttle in strange land to plant selection later, by improvement II Pedigree selection method selection, stringent antagonism and plant
Strain character is selected, and carries out fringe choosing when harvest, and continuous bag sleeving is from being sent to F5-6For when stabilize the excellent Suwan selfing of a batch
System, such as QB1923, QB2208.
Self-mating system includes T32, QB48, QR273 in the group of Suwan germplasm described in step 1 and step 2.By the method, i.e.,
Select excellent corn inbred line.
Implementation process of the invention：Circulation modification method in corn Suwan germplasm group,
Spring in 2004：It selects in production and is planted using preferable self-mating system T32 and QB48 in Kweiyang, assemble cenospecies,
Harvest F1For seed.
Winter in 2004：The F that a upper season is harvested1It is planted for seed in Hainan, florescence mixed pollination, when harvest selects
5 good fruit ears of robustness mix threshing, obtain F2For seed.
Spring in 2005：The F that a upper season is harvested2200- is planted by 3500-4000 plants/acre of density in Kweiyang for seed
300 plants, in the low single plant bagging selfing in florescence selection plant fringe position, harvest the good fruit ear of disease-resistant anti-fall and robustness, Dan Sui
Threshing obtains F3For seed.
Winter in 2005：The F that a upper season is harvested3It is planted by 3800-4100 plants/acre of density into fringe for seed in Hainan
Row, using the above method, in florescence selection single plant bagging selfing, each head progeny row selects excellent selfing fruit ear 3-5 a, and single fringe is de-
Grain obtains F4For seed.
Spring in 2006：The F that a upper season is harvested4Plantation is planted by 3900-4200 plants/acre of density in Kweiyang for seed
At head progeny row, continues to show in florescence and select the selfing of single plant bagging in excellent head progeny row using above-mentioned, harvest disease-resistant anti-fall and tie
The good fruit ear of reality, single fringe threshing obtain F5For seed.
Winter in 2006：The F that a upper season is harvested5Plantation is planted by 4000-4300 plants/acre of density in Hainan for seed
At head progeny row, continue to be selected using pedigree method, is showed in florescence and select the selfing of single plant bagging in excellent head progeny row, harvest knot
The good fruit ear of reality, single fringe threshing obtain F6For seed.
Spring in 2007：The F that a upper season is harvested6Plantation is planted by 4100-4500 plants/acre of density in Kweiyang for seed
At head progeny row, continue to show in florescence using the above method and select the selfing of single plant bagging in excellent head progeny row, harvest disease-resistant anti-fall
And the fruit ear that robustness is good, single fringe threshing obtain F7For seed.
Winter in 2007：The F that a upper season is harvested7Plantation is planted by 4200-4600 plants/acre of density in Hainan for seed
At head progeny row, excellent head progeny row is showed in florescence and carries out mixed pollination, and be measured with Testers；Harvest the good head progeny row 9 of robustness
A, head progeny row mixing threshing, number saves (QB569-QB578).
Spring in 2008：The seed that is measured of harvest of the upper season was subjected to multi-point combination identification in Guizhou, determines coordinate force
Good self-mating system is QB572 and QB576.
Winter in 2008：QB572 and QB576 is planted in Hainan, is selected in production using preferable self-mating system QR273 and its
Cenospecies is assembled, F is harvested1For seed.
Spring in 2009：The F that a upper season is harvested1It is planted for seed in Kweiyang, using improvement II pedigree selection method choosing
It selects, florescence mixed pollination is simultaneously returned, and 10 good fruit ears of selection robustness, mix threshing when harvest, obtains F2Or F1BC1
For seed.
Winter in 2009：A upper season is harvested into F2Or F1BC1It is planted in Hainan by 4000-4200 plants/acre of density for seed
200-300 plants, in florescence selection single plant bagging selfing, good 25, fruit ear of harvest robustness, single fringe threshing obtains F3Generation kind
Son.
Spring in 2010：The F that a upper season is harvested2It is planted by 4100-4300 plants/acre of density at 25 for seed in Xingyi
A head progeny row selects disease-resistant 20 anti-fall head progeny rows in florescence selection single plant bagging selfing, harvest, and each head progeny row selects robustness good
3, fruit ear, single fringe threshing, obtain F3For seed.
Winter in 2010：The F that a upper season is harvested3Plantation is planted by 4300-4500 plants/acre of density in Hainan for seed
It at 60 head progeny rows, is showed in florescence and selects the selfing of single plant bagging in excellent head progeny row, good 72, fruit ear of harvest robustness are single
Fringe threshing obtains F4For seed.
Spring in 2011：The F that a upper season is harvested4Plantation is planted by 4500-4700 plants/acre of density in Kweiyang for seed
It at 72 head progeny rows, is showed in florescence and selects the selfing of single plant bagging in excellent head progeny row, when harvest selects disease-resistant 50 anti-fall fringes
Row, each head progeny row select good 2, fruit ear of robustness, and single fringe threshing obtains F5For seed.
Winter in 2011：The F that a upper season is harvested5Plantation is planted by 4600-4800 plants/acre of density in Hainan for seed
At 100 head progeny rows, excellent head progeny row is showed in florescence and carries out mixed pollination, and be measured with Testers；It is good to harvest robustness
Head progeny row, head progeny row mixing threshing, number save.
Embodiment through the invention has finally selected a collection of excellent corn inbred line such as QB1923, QB2208 etc.,
It is existing to authorize corn variety that a collection of high yield, stable yields, wide suitable, high-quality, mostly anti-superior corn kind have been bred as by them：It is tall and erect
It is No. 2 beautiful (QB662 × 2219, national level authorization), gold and jade 579 (QB1013 × QB1923, Guizhou, the authorization of two province of Yunnan), hundred grand
Jade 988 (QB2398 × QB2208, Guizhou authorization) is being participated in area (pre-) Varieties 12.
By being identified after being hybridized using the several Suwan germplasm of the present invention with Testers PH6WC, combination yield is compared
Compared with, the self-mating system Q2232 being bred as using this method compared with the combination that QR273, QB48, T32 are assembled, increase production 8.6% respectively,
13.2%, 16.55%, illustrate that this method effectively raises yield combining ability.In addition, in terms of main agriculture character：It reduces
Plant height Ear height, plant type are reasonable, improve disease-resistant (inverse) property etc..
The Yield comparison of table 1Suwan germplasm and Testers PH6WC cross combination
Refer on the excellent corn inbred line of the above, production using excellent described in preferable Elite inbred：
(pressing breeding objective) resistance to lean, disease-resistant, wide adaptability of drought resisting, coordinate force is high and plant type is good, marketing quality is good, color is good, robustness
It is good.
Circulation improvement refers in group described above：Based in production using the self-mating system selected from Suwan group
Material, by " have complementary advantages, it is excellent it is middle select it is excellent " principle constantly tame in selecting, beneficial gene made gradually to add up, formed " gradually
The improvement of formula ".
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (2)
1. the circulation modification method in a kind of corn Suwan germplasm group, which is characterized in that include the following steps：
Step 1：Hybridize using selected from corn Suwan group and using preferable Elite inbred T32 in production with QB48, it will
Its F1After plantation, using improvement I Pedigree selection method selection, i.e. F1For mixed pollination, mixed planting, mixing threshing, do not select, F2
Start individual plant selfing, head progeny row plantation and selection, the selfing of incubation and does combining ability test；
Step 2：The F that step 1 is obtained2After seed plantation, by the selection method of step 1), stringent antagonism and tree characteristics into
Row selection, carries out fringe choosing when harvest, and continuous bag sleeving is from being sent to F6-8In generation, selects highest two self-mating systems of coordinate force；
Step 3：After two self-mating systems in step 2 are hybridized or are returned with from the QR273 of Suwan1C11 breeding again, by its F1Kind
After plant, using improvement II Pedigree selection method selection, i.e. F1In generation, does not select, mixed pollination and is returned, mixes threshing, hybrid
It plants, F2Or F1BC1In generation, starts individual plant selfing, head progeny row plantation and selection, F2In generation, starts to shuttle in strange land to plant selection later, strictly
Antagonism and tree characteristics are selected, and carry out fringe choosing when harvest, and continuous bag sleeving is from being sent to F5-6In generation, it is excellent to obtain a batch
Suwan self-mating system.
2. the circulation modification method in corn Suwan germplasm group according to claim 1, it is characterised in that：Institute in step 1
The selection stated refer to by embodiment selection principle and method in different times antagonism, plant type, florescence harmony, fruit
The characters such as fringe are strictly selected；Selection criteria described in step 3 is the same as step 2.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109729969A (en) * | 2019-01-15 | 2019-05-10 | 贵州省旱粮研究所 | The breeding method of the new monoid PLR germplasm of corn |
| CN110115226A (en) * | 2019-05-06 | 2019-08-13 | 贵州省旱粮研究所 | A method of based on corn Suwan germplasm breeding Day neutral self-mating system |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109729969A (en) * | 2019-01-15 | 2019-05-10 | 贵州省旱粮研究所 | The breeding method of the new monoid PLR germplasm of corn |
| CN110115226A (en) * | 2019-05-06 | 2019-08-13 | 贵州省旱粮研究所 | A method of based on corn Suwan germplasm breeding Day neutral self-mating system |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
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