CN105010150A - Matched cultivation method of stevia rebaudiana triploid hybrids - Google Patents
Matched cultivation method of stevia rebaudiana triploid hybrids Download PDFInfo
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- CN105010150A CN105010150A CN201510517749.8A CN201510517749A CN105010150A CN 105010150 A CN105010150 A CN 105010150A CN 201510517749 A CN201510517749 A CN 201510517749A CN 105010150 A CN105010150 A CN 105010150A
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- stevia
- tetraploid
- triploid
- stevia rebaudiana
- colchicine
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Abstract
The invention discloses a matched cultivation method of stevia rebaudiana triploid hybrids. The method comprises steps as follows: diploid materials of stevia rebaudiana are subjected to chromosome doubling, individual stevia rebaudiana plant materials of tetraploids and diploids to be subjected to matching hybridization are subjected to cutting propagation respectively, a large quantity of seed production parent strains of tetraploid and diploid stevia rebaudiana with consistent genetypes and reproductive traits are obtained respectively, the two seed production parent strains are planted in rows in the same field alternately for hybrid seed production, and due to the self-incompatible characteristic of the stevia rebaudiana, the stevia rebaudiana triploid hybrids are obtained after natural hybridization. According to the huge plant triploid organs, the leaf yield of the stevia rebaudiana can be remarkably increased with the adoption of the measure.
Description
Technical field
a supporting breeding method for stevia triploid hydrid kind, relates to stevia triploid Breeding technology, is that a kind of dliploid stevia and tetraploid stevia carry out hybridizing the method for cultivating triploid stevia crossbreed in particular.
Background technology
in crops, many crops have all been bred as triploid high yield and high quality new varieties, bring breakthrough raising to the yield and quality of crop.Its specific practice, for watermelon, is bred as seedling by the watermelon seed of zygoid and the tetraploid watermelon seed that isozygotys, is alternately planted on same soil, and allow them carry out the hybridization of orientation, namely the seed obtained is triploid watermelon seed.It is all adopt conventional kind or even the farm variety of various places that current stevia is produced, and yields poorly and unstable, and quality is uneven.Triploid plant has the feature of the huge property of reproductive organs, if can be used in stevia, the blade of stevia also can be made huger, thus obtains higher output.But due to characteristic and the botanical character of the hereditary reproduction of stevia self, the triploid breeding of stevia can not apply mechanically the method for other crop simply.Stevia is a kind of plant of strict cross pollination, has self-incompatibillity, but the pollen between different plants can be set seeds in phase mutual cross.If dliploid and tetraploid Stevia seed seed are implanted in same piece of land, not only dliploid and tetraploid can phase mutual crosses, also can pollinate hybridization mutually in Diploid and Tetraploid colony inside, so the seed produced will be disorderly and unsystematic, existing triploid crossbreed, also there are tetraploid and diplontic seed, colony is seriously mixed, agricultural production standardisation requirements can not be reached.Because flower amount is huge, fruit is very little, and the seed that every flower is tied is few, obviously cannot produce Stevia seed in a large number with manually carrying out hybridizing.How the dliploid of stevia and tetraploid being hybridized, produce the stevia triploid hydrid kind that genotype is all consistent with reproductive characteristic, is a difficult problem urgently to be resolved hurrily.
Summary of the invention
the object of the invention is to the above-mentioned difficult problem for stevia triploid Breeding exists, propose a kind of can hybridization by the Diploid and Tetraploid of stevia, produce the method for the genotype stevia triploid hydrid kind all consistent with reproductive characteristic.
the present invention is achieved in that
a supporting breeding method for stevia triploid hydrid kind, it comprises the steps:
(1) mutagenesis stevia tetraploid: double to become tetraploid with the dliploid material mutagenesis of colchicine by part stevia;
(2) stevia production of hybrid seeds parent strain is cultivated: the stevia individual plant material of Diploid and Tetraploid is carried out cuttage and expand numerous, breed out stevia production of hybrid seeds parent strain all consistent with reproductive characteristic from the genotype of single plant in a large number respectively;
(3) hybrid seeding: be planted in same Tanaka by alternate by row with dliploid stevia production of hybrid seeds parent strain for tetraploid stevia production of hybrid seeds parent's strain, utilize insect to carry out mutual hybridization pollination, the seed of results is triploid Stevia seed.
the described tetraploid method of mutagenesis stevia is: on superclean bench, take out test-tube plantlet removing blade, be cut into about 1 cm long containing side leaf stem section, be placed horizontally at regeneration culture medium on the surface, flicking is fixed, every bottle graft kind 5 stem sections, each process inoculation 10 bottles, finally be placed in after culturing room carries out cultivation cultivation 2 d and take out, superclean bench drips 0.025% colchicine (filtration sterilization), guarantee that colchicine infiltrates each stem section, colchicine is disposed and puts back to culturing room, when continuation cultivation bud seedling grows to 2.0 cm height, (about 30 d), bud seedling is cut to be inoculated in root media by superclean bench and cultivates 20 d, seize and double successful tetraploid plant.
described regeneration culture medium is leaf regeneration seedling medium is MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+ agar powder 5.0 g/L+ sucrose 30 g/L; Culture of rootage is root media is MS+IBA 0.5 mg/L+ agar powder 5.0 g/L+ sucrose 20 g/L.
beneficial effect of the present invention:
the present invention proposes under conditions of tissue culture, with colchicine, the induction of part stevia material is doubled the body that quadruples, again respectively by the method for group training, the production of hybrid seeds parent strain of the single Diploid and Tetraploid of numerous successful genotype is expanded in amplification in enormous quantities by single plant.Its genotype separately in colony between individuality is just the same, is all that a stevia individual plant " is attended to anything else ", reproductive characteristic can be considered an individual plant.Because stevia has self-incompatible characteristic, not only individual selfing can not be solid for such production of hybrid seeds parent strain, can not mutually pollinate solid between the plant of its colony inside separately.The Diploid and Tetraploid stevia seed cultivated like this is implanted in together, when blooming, only has between the plant of Diploid and Tetraploid and can phase mutual cross set seeds, so just obtain genotype triploid Stevia seed all consistent with reproductive characteristic.This method effectively prevent the hybridization of Stevia seed seedling, and the plant phase mutual cross in the parental population of existence, causes the problem that seed seriously mixes.Because triploid plant has the rule of the huge property of organ, thus the output of stevia is significantly improved.
Embodiment
cultivate the step of stevia triploid hydrid kind:
(1) mutagenesis stevia tetraploid: double to become tetraploid with the dliploid material mutagenesis of colchicine by part stevia;
(2) stevia production of hybrid seeds parent strain is cultivated: the stevia individual plant material of Diploid and Tetraploid is carried out cuttage and expand numerous, breed out stevia production of hybrid seeds parent strain all consistent with reproductive characteristic from the genotype of single plant in a large number respectively;
(3) hybrid seeding: be planted in same Tanaka by alternate by row with dliploid stevia production of hybrid seeds parent strain for tetraploid stevia production of hybrid seeds parent's strain, utilize insect to carry out mutual hybridization pollination, the seed of results is triploid Stevia seed.
the tetraploid method of mutagenesis stevia is: on superclean bench, take out test-tube plantlet removing blade, be cut into about 1 cm long containing side leaf stem section, be placed horizontally at regeneration culture medium on the surface, flicking is fixed, every bottle graft kind 5 stem sections, each process inoculation 10 bottles, finally be placed in after culturing room carries out cultivation cultivation 2 d and take out, superclean bench drips 0.025% colchicine (filtration sterilization), guarantee that colchicine infiltrates each stem section, colchicine is disposed and puts back to culturing room, when continuation cultivation bud seedling grows to 2.0 cm height, (about 30 d), bud seedling is cut to be inoculated in root media by superclean bench and cultivates 20 d, seize and double successful tetraploid plant.
regeneration culture medium is leaf regeneration seedling medium is MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+ agar powder 5.0 g/L+ sucrose 30 g/L; Culture of rootage is root media is MS+IBA 0.5 mg/L+ agar powder 5.0 g/L+ sucrose 20 g/L.
Claims (3)
1. a supporting breeding method for stevia triploid hydrid kind, it is characterized in that, it comprises the steps:
(1) mutagenesis stevia tetraploid: double to become tetraploid with the dliploid material mutagenesis of colchicine by part stevia;
(2) stevia production of hybrid seeds parent strain is cultivated: the stevia individual plant material of Diploid and Tetraploid is carried out cuttage and expand numerous, breed out stevia production of hybrid seeds parent strain all consistent with reproductive characteristic from the genotype of single plant in a large number respectively;
(3) hybrid seeding: be planted in same Tanaka by alternate by row with dliploid stevia production of hybrid seeds parent strain for tetraploid stevia production of hybrid seeds parent's strain, utilize insect to carry out mutual hybridization pollination, the seed of results is triploid Stevia seed.
2. the supporting breeding method of a kind of stevia triploid hydrid kind according to claim 1, it is characterized in that, the described tetraploid method of mutagenesis stevia is: on superclean bench, take out test-tube plantlet removing blade, be cut into about 1 cm long containing side leaf stem section, be placed horizontally at regeneration culture medium on the surface, flicking is fixed, every bottle graft kind 5 stem sections, each process inoculation 10 bottles, finally be placed in after culturing room carries out cultivation cultivation 2 d and take out, superclean bench drips 0.025% colchicine (filtration sterilization), guarantee that colchicine infiltrates each stem section, colchicine is disposed and puts back to culturing room, when continuation cultivation bud seedling grows to 2.0 cm height, (about 30 d), bud seedling is cut to be inoculated in root media by superclean bench and cultivates 20 d, seize and double successful tetraploid plant.
3. the supporting breeding method of a kind of stevia triploid hydrid kind according to claim 2, it is characterized in that, described regeneration culture medium is leaf regeneration seedling medium is MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+ agar powder 5.0 g/L+ sucrose 30 g/L; Culture of rootage is root media is MS+IBA 0.5 mg/L+ agar powder 5.0 g/L+ sucrose 20 g/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510517749.8A CN105010150A (en) | 2015-08-23 | 2015-08-23 | Matched cultivation method of stevia rebaudiana triploid hybrids |
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| CN201510517749.8A CN105010150A (en) | 2015-08-23 | 2015-08-23 | Matched cultivation method of stevia rebaudiana triploid hybrids |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613954A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Cultivation method for stevia rebaudiana tetraploid |
| CN107211881A (en) * | 2017-05-22 | 2017-09-29 | 河南师范大学 | A kind of method of glutinous rehmannia crossbreeding |
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2015
- 2015-08-23 CN CN201510517749.8A patent/CN105010150A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613954A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Cultivation method for stevia rebaudiana tetraploid |
| CN107211881A (en) * | 2017-05-22 | 2017-09-29 | 河南师范大学 | A kind of method of glutinous rehmannia crossbreeding |
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Application publication date: 20151104 |
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