CN106718925B - A kind of roxburgh anoectochilus terminal bud field rapid propagation method - Google Patents
A kind of roxburgh anoectochilus terminal bud field rapid propagation method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention relates to a kind of roxburgh anoectochilus terminal bud field rapid propagation method, platymiscium fast breeding technique fields；Six steps of daily management and harvesting after substrate composition used in preservation, breeding including reserved maternal plant and disinfection, breeding cuttings processing, reasonable cuttage dense planting, plant；The technology simplifies roxburgh anoectochilus terminal bud by perennial root breeding and outdoor cuttage complex art and breeds process, improves its reproductive efficiency, and can effectively reduce production cost.
Description
Technical field
The present invention relates to a kind of rapid breeding method of plant, and in particular to a kind of roxburgh anoectochilus terminal bud field rapid propagation method belongs to
In micropropagation of plants technical field.
Background technique
Roxburgh anoectochilus terminal bud (Anoectochilusroxburghii) it is rare orchidaceae medicinal plant, there are a variety of significant medicines
With value and health-care efficacy.But since the development and application of roxburgh anoectochilus terminal bud are started late, still fail so far as common Chinese medicine simply
Material and be more widely applied.How by improving breeding method and improving reproduction technique, to reduce cost, upgrading increases
Effect, it is the project for needing to solve that it is widely used to a greater extent.
The research of roxburgh anoectochilus terminal bud fast breeding technique is mainly reflected in the exploitation of tissue culture technology and improves, as CN101213942 is related to
Tissue culture once-seedling forming technology, CN103416305A is related to no hormone tissue culture and quick-breeding method, CN20121044259318 are related to group
Stem section reproduction method is trained, CN201310709608.7 is related to the tissue culture propagation mode of fruit pod sowing, and CN201310083379.2 is related to
And the method that organic matter improves breeding potential is added during tissue culture, CN200910154637.5 is related to stem apex and stem section tissue culture propagation
Method.The main means that always plant factor's metaplasia produces are bred using tissue culture technology, belong to fund and labor-intensive
Industry, manpower and material resources consuming is huge, and plant longer for growth period is an extraordinary Propagation Methods；But for roxburgh anoectochilus terminal bud
The plant that growth periods are short is waited, can choose more succinct quick propagation method, by avoiding tissue culture propagation approach from reducing cost
While improve reproductive efficiency.
Plant vegetative propagation approach include tissue culture technology, outdoor cuttage and press strip etc. fields reproduction technique.So far, golden
Line lotus field propagation method is only shown in that cutting stem involved in CN200810059998.7 report stays root regeneration implantation methods, and the invention
What is involved is a kind of planting technologies.Roxburgh anoectochilus terminal bud habit: 3 to the August speed of growth than very fast, and plant strain growth is vigorous, plant height,
Diameter, fresh weight all obviously increase, and into September part, roxburgh anoectochilus terminal bud growth gradually slows down, and plant top shows bud, December in November-roxburgh anoectochilus terminal bud
Output white little Hua.Roxburgh anoectochilus terminal bud is not suitable for press strip, therefore can pass through perennial root breeding and outdoor according to the season of growth of roxburgh anoectochilus terminal bud
Cuttage technique is the key technology means for improving its reproductive efficiency.
Summary of the invention
The object of the present invention is to provide a kind of roxburgh anoectochilus terminal bud field perennial roots to breed the synthesis field quick-breeding method with cutting propagation,
Reproductive-cost in roxburgh anoectochilus terminal bud production process is reduced by field reproduction technique, improves reproductive efficiency.
For realize foregoing invention purpose, the technical solution adopted by the present invention is as follows:
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, includes the following steps:
(1) maternal plant saves
Complete stool saves: in harvest season, that is, September to October, retaining small part plant and does not harvest, it is numerous to give over to March in next year
Grow material；
Perennial root saves: harvesting overground part plant before plant bolting is bloomed, retains one stipes of basal part of stem, after harvesting immediately
It is sprayed with streptomysin and continues to employ maternal plant, sprayed potassium dihydrogen phosphate after a week and amino acid carries out management of keeping a full stand of seedings；
(2) matrix prepares
Substrate composition: peat soil, river sand, perlite carry out preparation cultivation matrix according to 10:1:2 proportion, or using new
China fir sawdust；
Matrix disinfection: the preposition solarization under sunlight is used, 5~7.5 kilograms of addition is ripe in subsequent every 100 kilograms of cultivation matrixes
Lime or 50% thiophanate methyl or 30% streptomysin are mixed thoroughly, and matrix is mixed thoroughly with clear water, it is desirable that profit without wet, stromal thickness is 8~
10cm；
(3) cuttings is handled
Terminal bud cuttings: branch terminal bud defoliation retains to 2-3 piece leaf；
Stem section cuttings: it will be 3~4cm stem section with axillary bud branch sheared length, and remove blade；
Pretreatment: the terminal bud and stem section cuttings sheared impregnate 30-60s with 4000~5000 times of streptomysins, then use
100ppm IBA and IAA impregnate 5~15min；
(4) cuttage dense planting
Terminal bud dense planting: soaked terminal bud cuttings is inserted directly into the matrix prepared, is planted by seeding row spacing 5cm × 5cm kind, often
A plantation square plate transplants 140~150 plants, and the depth of planting is 1cm or so；
Stem section cuttage: soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation
Square plate transplants 80~90 stem sections, and depth of planting 3-5mm, matrix 2-3mm is covered on surface layer；
(5) Planting management
Routine Management: it is sprayed after cultivation with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, according to weather after inserting
Situation is sprayed water moisturizing in due course, controls 70% or more air humidity, and soil humidity maintains 70~75%, and temperature is at 20~27 DEG C, light
According to intensity 2000-3000lux, 1 nutrient solution is sprayed within 7~10 days；
Prevent and treat pest and disease damage: greenhouse all around upper fly net traps and kills black cutworm using sugar-vinegar liquid, or using bacterium, fungi,
The biological pesticides such as virus, plant leaching liquor immersion, antibiotic kill disease pest；
(6) it harvests
Harvesting standard: after cuttings transplants 7-8 months, the high 10~15cm or more of plant has 5~6 leaves, and leaf width 2~
2.5cm, leaf is 3~3.5 centimetres long, and blade back is in aubergine, and the blade face network of vein is obvious, can harvest when stem is in green；
Collecting method: complete stool is partially left and taken as next year propagation material, remaining whole locality upper bit retains basal part of stem
One stipes, is sprayed immediately with streptomysin after harvesting and continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid is kept a full stand of seedings
It is managed with germ tube is promoted.
2, a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that: step (1) institute
The complete stool stated saves and perennial root saves, and cuts its overground part March in next year and makees cuttings cultivation new talent, and underground part is numerous as perennial root
It grows.
Step (3) and step (4) carry out in March in next year.
The processing of cuttings described in step (4) is that overground part branch is divided into two kinds of cuttage materials of stem apex and stem section to classify
Cultivation management.
Plant described in step (5) includes terminal bud cuttings and stem section cuttings, and the terminal bud cuttings is to open for 1 week after plant
Beginning sprays 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 time, sprays within 7-10 days once, after sprouting new root, sprays within 7~10 days 1 N:P:
K ratio is the nutrient solution of 20:20:20, gradually increases illumination, intensity control is in 3000lux or so.The stem section cuttings plant side
Formula is that cuttings is placed horizontally in matrix；Start within 1 week after plant to spray the nutrient solution that 1 N:P:K ratio is 30:10:10, sprout
After sprouting, the nutrient solution that 1 N:P:K ratio is 20:20:20 is sprayed within 7~10 days, gradually increases illumination, intensity control is in 2000-
3000lux。
After harvesting described in step (6), kept a full stand of seedings every 7-10 days to complete stool sprays 45% amino acid, 500 times of liquid, often
1000 times of liquid of potassium dihydrogen phosphate were sprayed to perennial root maternal plant every 7-10 days to carry out promoting germ tube reason.
The beneficial effects of the present invention are:
(1) roxburgh anoectochilus terminal bud is simplified by perennial root breeding and outdoor cuttage complex art and breeds process, improve its reproductive efficiency.
(2) complete stool preserving type, which avoids emergency situations, leads to the uncertainty of seedling, to ensure that cuttage material
It supplies year after year, perennial root preserving type provides reliable guarantee to carry out annual output increase.
(3) the high cost problem of the emergency crops tissue culture the factorial production such as roxburgh anoectochilus terminal bud is solved, seedling cost is significantly
It reduces, to improve economic benefit.
Specific embodiment
The present invention is described in further details below by example, these examples are only used to illustrate the present invention, and unlimited
The scope of the present invention processed.
Embodiment 1
A kind of roxburgh anoectochilus terminal bud field rapid propagation method, includes the following steps:
(1) maternal plant saves
Perennial root saves: harvesting overground part plant before plant bolting is bloomed, retains one stipes of basal part of stem, harvest after harvesting
Afterwards, reservation basal part of stem is sprayed with 30% agricultural streptomycin, 4000~5000 times of liquid immediately, 2-3 hypophosphorous acid potassium dihydrogen is sprayed after 1 week
1000 times of liquid, 7-10d are sprayed 1 time.After harvesting 2 weeks, perennial root material is sprayed with 45% amino acid, 500 times of liquid, is sprayed within 7-10 days 1 time,
Carry out management of keeping a full stand of seedings.
(2) matrix prepares
Use new China fir sawdust as cultivation matrix；
Matrix disinfection: the preposition solarization under sunlight is used, 5 kilograms of white limes are added in subsequent every 100 kilograms of cultivation matrixes
It mixes thoroughly, matrix is mixed thoroughly with clear water, it is desirable that for profit without wet, stromal thickness is 8~10cm；
(3) cuttings is handled
Branch terminal bud defoliation is retained to 2-3 piece leaf using terminal bud cuttings；
(4) cuttage dense planting
Terminal bud dense planting: soaked terminal bud cuttings is inserted directly into the matrix prepared, is planted by seeding row spacing 5cm × 5cm kind, often
A plantation square plate transplants 140~150 plants, and the depth of planting is 1cm or so；
(5) Planting management
Overwinter management: 10 DEG C of temperature of control or more, initial intensity of illumination 1500-2000lux, air humidity 50%~70% are left
It is right；
Promote germ tube reason: month in next year 2-3, control 70% or more air humidity, temperature sprays 1 in 20~28 DEG C, 7~10 days
Secondary N:P:K(30:10:10) nutrient solution；
Daily management: 70% or more air humidity of control, temperature spray 1 N:P:K(20 for 7~10 days at 20~28 DEG C:
20:20) nutrient solution；
Prevent and treat pest and disease damage: greenhouse all around goes up fly net, traps and kills black cutworm using sugar-vinegar liquid, utilizes bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
(6) it harvests
Harvesting standard: after cuttings transplants 7-8 months, the high 10~15cm or more of plant has 5~6 leaves, and leaf width 2~
2.5cm, leaf is 3~3.5 centimetres long, and blade back is in aubergine, and the blade face network of vein is obvious, can harvest when stem is in green；
Collecting method: complete stool is partially left and taken as next year propagation material, remaining whole locality upper bit retains basal part of stem
One stipes, is sprayed immediately with streptomysin after harvesting and continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid is kept a full stand of seedings
It is managed with germ tube is promoted.
Embodiment 2
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, includes the following steps:
(1) maternal plant saves
Complete stool saves: in harvest season, that is, September to October, retaining small part plant and does not harvest, it is numerous to give over to March in next year
Grow material；
(2) matrix prepares
Peat soil: river sand: perlite=10:1:2 proportion carries out preparation cultivation matrix；
Matrix disinfection: the preposition solarization under sunlight is used, 7.5 kilograms of ripe stones are added in subsequent every 100 kilograms of cultivation matrixes
Ash or suitable 50% thiophanate methyl or 30% streptomysin are mixed thoroughly, and matrix requires profit without wet, and water content is controlled to 70% left side
It is right；
Medium in tray: matrix is installed with rectangular planting tray, and stromal thickness is 8~10cm；
(3) cuttings is handled
It will be 3~4cm stem section with axillary bud branch sheared length, and remove blade, soaked with 4000~5000 times of agricultural streptomycins
1min is steeped, then impregnates 10~15min with 100ppm IBA and IAA；
(4) cuttage dense planting
Soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation square plate plant 80
Matrix 2-3mm is covered on~90 stem sections, depth of planting 3-5mm, surface layer
(5) Planting management
Routine Management: it is sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, according to weather condition after inserting
Water spray moisturizing in due course controls 70% or more air humidity, and soil humidity maintains 70~75%, and temperature is at 20~28 DEG C, intensity of illumination
Control is in 1000-1500lux；
Promote germ tube reason: starting within 1 week after plant to spray 1 N:P:K(30:10:10) nutrient solution, after sprouting sprouting, 7~10 days
Spray 1 N:P:K(20:20:20) nutrient solution, illumination is gradually increased, intensity control is in 2000-3000lux.
Prevent and treat pest and disease damage: greenhouse all around goes up fly net, traps and kills black cutworm using sugar-vinegar liquid, utilizes bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
(6) it harvests
Harvesting standard: after cuttings transplants 7-8 months, the high 10~15cm or more of plant has 5~6 leaves, and leaf width 2~
2.5cm, leaf is 3~3.5 centimetres long, and blade back is in aubergine, and the blade face network of vein is obvious, can harvest when stem is in green；
Collecting method: complete stool is partially left and taken as next year propagation material, remaining whole locality upper bit retains basal part of stem
One stipes, is sprayed immediately with streptomysin after harvesting and continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid is kept a full stand of seedings
It is managed with germ tube is promoted.
Embodiment 3
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, includes the following steps:
(1) maternal plant saves
Complete stool saves: in harvest season, that is, September to October, retaining small part plant and does not harvest, it is numerous to give over to March in next year
Grow material；
(2) matrix prepares
Peat soil: river sand: perlite=10:1:2 proportion carries out preparation cultivation matrix；
Matrix disinfection: the preposition solarization under sunlight is used, 5~7.5 kilograms of addition is ripe in subsequent every 100 kilograms of cultivation matrixes
Lime or suitable 50% thiophanate methyl or 30% streptomysin are mixed thoroughly, and matrix requires profit without wet, and water content is controlled to 70% left side
It is right；
Medium in tray: matrix is installed with rectangular planting tray, and stromal thickness is 8~10cm；
(3) cuttings is handled
Retain branch terminal bud defoliation to top 2-3 piece leaf, impregnate 1min with 4000~5000 times of agricultural streptomycins,
10~15min is impregnated with 100ppm IBA and IAA again；
(4) cuttage dense planting
Soaked terminal bud cuttings is inserted directly into the matrix prepared, is planted by seeding row spacing 5cm × 5cm kind, each plantation side
Disk transplants 140~150 plants, and the depth of planting is 1cm or so；
(5) Planting management
Routine Management: it is sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, according to weather condition after inserting
Water spray moisturizing in due course controls 70% or more air humidity, and soil humidity maintains 70~75%, and temperature is at 20~28 DEG C, intensity of illumination
Control is in 1500-2000lux；
Promoting root growth management: starting to spray 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 times for 1 week after plant, spray within 7-10 days it is primary,
After sprouting new root, 1 N:P:K(20:20:20 is sprayed within 7~10 days) nutrient solution, illumination is gradually increased, intensity control is in 3000lux
Left and right；
Prevent and treat pest and disease damage: greenhouse all around goes up fly net, and sugar-vinegar liquid is utilized to trap and kill black cutworm；
(6) it harvests
Harvesting standard: after cuttings transplants 7-8 months, the high 10~15cm or more of plant has 5~6 leaves, and leaf width 2~
2.5cm, leaf is 3~3.5 centimetres long, and blade back is in aubergine, and the blade face network of vein is obvious, can harvest when stem is in green；
Collecting method: complete stool is partially left and taken as next year propagation material, remaining whole locality upper bit retains basal part of stem
One stipes, is sprayed immediately with streptomysin after harvesting and continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid is kept a full stand of seedings
It is managed with germ tube is promoted.
Claims (3)
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, characterized by the following steps:
(1) maternal plant saves
Complete stool saves: in harvest season, that is, September to October, retaining small part plant and does not harvest, gives over to breeding in March in next year material
Material；
Perennial root saves: harvesting overground part plant before plant bolting is bloomed, retains one stipes of basal part of stem, use chain after harvesting immediately
Mycin, which sprays, continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid carries out management of keeping a full stand of seedings；
(2) matrix prepares
Substrate composition: peat soil, river sand, perlite carry out preparation cultivation matrix according to 10:1:2 proportion, or use new China fir
Sawdust；
Matrix disinfection: the preposition solarization under sunlight is used, 5~7.5 kilograms of white limes are added in subsequent every 100 kilograms of cultivation matrixes
Or 50% thiophanate methyl or 30% streptomysin mix thoroughly, matrix is mixed thoroughly with clear water, it is desirable that for profit without wet, stromal thickness is 8~10cm；
(3) cuttings is handled
Terminal bud cuttings: branch terminal bud defoliation retains to 2-3 piece leaf；
Stem section cuttings: it will be 3~4cm stem section with axillary bud branch sheared length, and remove blade；
Pretreatment: the terminal bud and stem section cuttings sheared impregnate 30-60s with 4000~5000 times of streptomysins, then use
100ppm indolebutyric acid IBA and indole-3-acetic acid IAA impregnates 5~15min；
(4) cuttage dense planting
Terminal bud dense planting: soaked terminal bud cuttings is inserted directly into the matrix prepared, is planted by seeding row spacing 5cm × 5cm kind, each kind
It plants square plate and transplants 140~150 plants, depth of planting 1cm；
Stem section cuttage: soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation square plate
80~90 stem sections are transplanted, depth of planting 3-5mm, matrix 2-3mm is covered on surface layer；
(5) Planting management
Routine Management: it is sprayed after cultivation with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, according to weather condition after inserting
Water spray moisturizing in due course controls 70% or more air humidity, and soil humidity maintains 70~75%, and for temperature at 20~27 DEG C, illumination is strong
2000-3000lux is spent, 1 nutrient solution is sprayed within 7~10 days；
Prevent and treat pest and disease damage: greenhouse all around goes up fly net, utilizes sugar-vinegar liquid to trap and kill black cutworm, or utilize bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
(6) it harvests
Harvesting standard: after cuttings transplants 7-8 months, the high 10~15cm or more of plant has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is 3~3.5 centimetres long, and blade back is in aubergine, and the blade face network of vein is obvious, can harvest when stem is in green；
Collecting method: complete stool is partially left and taken as next year propagation material, remaining whole locality upper bit retains basal part of stem one
Stipes, is sprayed immediately with streptomysin after harvesting and continues to employ maternal plant, sprays potassium dihydrogen phosphate after a week and amino acid is kept a full stand of seedings and promoted
Germ tube reason；
Complete stool described in step (1) saves and perennial root saves, and cuts its overground part March in next year and makees cuttings cultivation new talent, ground
It is bred as perennial root lower part；
Step (3) and step (4) carry out in March in next year；
The processing of cuttings described in step (3) is that overground part branch is divided into two kinds of cuttage materials of stem apex and stem section to carry out classification cultivation
Management；
Plant described in step (5) includes terminal bud cuttings and stem section cuttings；
Its method is as follows: the terminal bud cuttings is to start within 1 week after plant to spray 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 times,
It sprays within 7-10 days once, after sprouting new root, sprays within 7~10 days the nutrient solution that 1 N:P:K ratio is 20:20:20, gradually increase light
According to intensity of illumination 2000-3000lux.
2. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that: the stem section cuttings
Planting system is that cuttings is placed horizontally in matrix；Start within 1 week after plant to spray the nutrition that 1 N:P:K ratio is 30:10:10
Liquid after sprouting sprouting, sprays the nutrient solution that 1 N:P:K ratio is 20:20:20 for 7~10 days, gradually increases illumination, intensity control exists
2000-3000lux。
3. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that: described in step (6)
After harvesting, kept a full stand of seedings every 7-10 days to complete stool sprays 45% amino acid, 500 times of liquid, perennial root maternal plant is sprayed every 7-10 days
1000 times of liquid of potassium dihydrogen phosphate are applied to carry out promoting germ tube reason.
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|---|---|---|---|---|
| CN1799344A (en) * | 2006-01-06 | 2006-07-12 | 浙江省农业科学院 | Artificial quick propagation method for hemsley rockvine root |
| CN101233808A (en) * | 2008-03-07 | 2008-08-06 | 浙江省中药研究所有限公司 | Medicinal anoectochilus formosan stem-cutting root-retaining regeneration planting method |
| CN102823412A (en) * | 2012-09-17 | 2012-12-19 | 地缘（厦门）生物科技有限公司 | Anoectochilus roburghii pollution-free planting method |
| CN102907305A (en) * | 2012-11-01 | 2013-02-06 | 黔西南州绿缘动植物科技开发有限公司 | Anoectochilus roxburghii wood medium cultivation technology |
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- 2017-01-04 CN CN201710005338.XA patent/CN106718925B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799344A (en) * | 2006-01-06 | 2006-07-12 | 浙江省农业科学院 | Artificial quick propagation method for hemsley rockvine root |
| CN101233808A (en) * | 2008-03-07 | 2008-08-06 | 浙江省中药研究所有限公司 | Medicinal anoectochilus formosan stem-cutting root-retaining regeneration planting method |
| CN102823412A (en) * | 2012-09-17 | 2012-12-19 | 地缘（厦门）生物科技有限公司 | Anoectochilus roburghii pollution-free planting method |
| CN102907305A (en) * | 2012-11-01 | 2013-02-06 | 黔西南州绿缘动植物科技开发有限公司 | Anoectochilus roxburghii wood medium cultivation technology |
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