CN106718925A - A kind of roxburgh anoectochilus terminal bud field rapid propagation method - Google Patents
A kind of roxburgh anoectochilus terminal bud field rapid propagation method Download PDFInfo
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- CN106718925A CN106718925A CN201710005338.XA CN201710005338A CN106718925A CN 106718925 A CN106718925 A CN 106718925A CN 201710005338 A CN201710005338 A CN 201710005338A CN 106718925 A CN106718925 A CN 106718925A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention relates to a kind of roxburgh anoectochilus terminal bud field rapid propagation method, platymiscium fast breeding technique field；Including six steps of daily management and harvesting after preservation, breeding substrate composition used and sterilization, the treatment of breeding cuttings, reasonable cuttage dense planting, the plant of reserving maternal plant；The technology is bred by perennial root and outdoor cuttage complex art simplifies roxburgh anoectochilus terminal bud and breeds flow, improves its reproductive efficiency, and can effectively reduce production cost.
Description
Technical field
The present invention relates to a kind of rapid breeding method of plant, and in particular to a kind of roxburgh anoectochilus terminal bud field rapid propagation method, category
In micropropagation of plants technical field.
Background technology
Roxburgh anoectochilus terminal bud（Anoectochilusroxburghii）It is rare orchidaceae medicinal plant, with various significant medicines
With value and health-care efficacy.But because the development and application of roxburgh anoectochilus terminal bud are started late, still fail so far as Chinese medicine conventional simply
Material and be more widely applied.How by improving breeding method and improving reproduction technique, so that reduces cost, upgrading increasing
Effect so that it is the problem for needing to solve that it can be widely used to a greater extent.
The research of roxburgh anoectochilus terminal bud fast breeding technique is mainly reflected in the exploitation of tissue culture technology and improves, and such as CN101213942 is related to
Tissue culture once-seedling forming technology, CN103416305A is related to without hormone tissue culture and quick-breeding method, and CN20121044259318 is related to group
Training Stem section reproduction method, CN201310709608.7 is related to the tissue culture propagation mode that Fruit pod is sowed, and CN201310083379.2 is related to
And the method that organic matter improves breeding potential is added during tissue culture, CN200910154637.5 is related to stem apex and stem section tissue culture propagation
Method.The Main Means that always plant factor's metaplasia is produced are bred using tissue culture technology, is belonged to fund and labor-intensive
Industry, manpower and materials expend huge, and the plant more long for growth period is an extraordinary Propagation Methods；But for roxburgh anoectochilus terminal bud
Deng the plant that growth period is short, more succinct quickly propagation method can be selected, by avoiding tissue culture propagation approach reduces cost
While improve reproductive efficiency.
Plant vegetative propagation approach include tissue culture technology, outdoor cuttage and field reproduction technique etc. press strip.So far, gold
Line lotus field propagation method is only shown in be related to cut stem in CN200810059998.7 reports and stays root regeneration implantation methods, and the invention
Refer to a kind of planting technology.Roxburgh anoectochilus terminal bud habit：3 to August speed of growth ratio is very fast, and plant strain growth is vigorous, plant height,
Footpath, fresh weight all substantially increase, and into September part, roxburgh anoectochilus terminal bud growth gradually slows down, the existing bud in plant top, November-December roxburgh anoectochilus terminal bud
Output white little Hua.Roxburgh anoectochilus terminal bud is not suitable for press strip, therefore can be bred by perennial root and outdoor according to the season of growth of roxburgh anoectochilus terminal bud
Cuttage technique is the key technology means for improving its reproductive efficiency.
The content of the invention
It is an object of the invention to provide a kind of breeding of roxburgh anoectochilus terminal bud field perennial root and the comprehensive field quick-breeding method of cutting propagation,
By reproductive-cost in field reproduction technique reduction roxburgh anoectochilus terminal bud production process, reproductive efficiency is improved.
To realize the purpose of foregoing invention, the technical scheme that the present invention takes is as follows：
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, comprises the following steps：
（1）Maternal plant preserves
Complete stool preserves：It is September to October in the harvest season, retains small part plant and do not harvest, gives over to March in next year breeding material
Material；
Perennial root is preserved：Overground part plant is harvested before plant bolting is bloomed, retains one stipes of basal part of stem, chain is used immediately after harvesting
Mycin is sprayed continues to employ maternal plant, and potassium dihydrogen phosphate and amino acid are sprayed after one week carries out management of keeping a full stand of seedings；
（2）Matrix prepares
Substrate composition：Peat soil, river sand, perlite are according to 10：1：2 proportionings carry out preparation cultivation matrix, or use new China fir
Wood chip；
Matrix is sterilized：Using preposition in Exposure to Sunlight under sunlight, 5~7.5 kilograms of white limes are added in subsequent every 100 kilograms of cultivation matrixes
Or 50% thiophanate methyl or 30% streptomysin mix thoroughly, matrix is mixed thoroughly with clear water, it is desirable to which without wet, stromal thickness is 8~10cm to profit；
（3）Cuttings treatment
Terminal bud cuttings：Retain branch terminal bud defoliation to 2-3 piece leaves；
Stem section cuttings：To be 3~4cm stem sections with axillary bud branch sheared length, remove blade；
Pretreatment：The terminal bud and stem section cuttings sheared soak 30-60s with 4000~5000 times of streptomysins, then use
100ppm IBA and IAA soak 5~15min；
（4）Cuttage dense planting
Terminal bud dense planting：Soaked terminal bud cuttings is inserted directly into the matrix for preparing, and is planted by seeding row spacing 5cm × 5cm kinds, each kind
Plant square plate to transplant 140~150 plants, the depth of planting is 1cm or so；
Stem section cuttage：Soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation square plate
80~90 stem sections of plant, the depth of planting is 3-5mm, and matrix 2-3mm is covered on top layer；
（5）Planting management
Routine Management：Sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid after cultivation, according to weather condition after inserting
Water spray moisturizing in good time, controls air humidity more than 70%, and soil humidity maintains 70~75%, and temperature is at 20~27 DEG C, and illumination is strong
Degree 2000-3000lux, sprays 1 nutrient solution for 7~10 days；
Prevention and elimination of disease and pests：Greenhouse all around goes up fly net, and black cutworm is trapped and killed using sugar-vinegar liquid, or using bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
（6）Harvesting
Harvesting standard：After cuttings plant 7-8 months, plant 10~more than 15cm high has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is long 3~3.5 centimetres, and blade back is in aubergine, and the blade face network of vein substantially, can harvesting when stem is in green；
Collecting method：Complete stool is left and taken as next year propagating materials in part, remaining whole locality upper bit, retains basal part of stem one
Stipes, is sprayed with streptomysin and continues to employ maternal plant immediately after harvesting, potassium dihydrogen phosphate is sprayed after one week and amino acid is kept a full stand of seedings and promoted
Germ tube is managed.
2nd, a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（1）Institute
The complete stool stated is preserved and perennial root is preserved, and its overground part is cut and makees cuttings cultivation new talent by March in next year, and underground part is numerous as perennial root
Grow.
Step（3）And step（4）Carried out in March in next year.
Step（4）Described cuttings treatment is that overground part branch is divided into two kinds of cuttage materials of stem apex and stem section to be classified
Cultivation management.
Step（5）Described plant includes terminal bud cuttings and stem section cuttings, and described terminal bud cuttings is to open for 1 week after plant
Beginning sprays 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 times, sprays within 7-10 days once, after sprouting new root, sprays within 7~10 days 1 N:P:
K ratios are 20:20:20 nutrient solution, gradually strengthens illumination, and intensity control is in 3000lux or so.Described stem section cuttings plant side
Formula is that cuttings is placed horizontally in matrix；Start within 1 week after plant to spray 1 N:P:K ratios are 30:10:10 nutrient solution, sprouts
After sprouting, 1 N is sprayed within 7~10 days:P:K ratios are 20:20:20 nutrient solution, gradually strengthens illumination, and intensity control is in 2000-
3000lux。
Step（6）After described harvesting, complete stool sprays 45% amino acid, 500 times of liquid are kept a full stand of seedings every 7-10 days, often
Spraying 1000 times of liquid of potassium dihydrogen phosphate to perennial root maternal plant every 7-10 days carries out promoting germ tube reason.
The beneficial effects of the invention are as follows：
（1）Bred by perennial root and outdoor cuttage complex art simplifies roxburgh anoectochilus terminal bud and breeds flow, improve its reproductive efficiency.
（2）Complete stool preserving type avoids emergency situations causes the uncertainty of seedling, so as to ensure that cuttage material
Supply year after year, perennial root preserving type provides Reliable guarantee to carry out annual production increase.
（3）The high cost of the emergency crop tissue culture factorial praluction such as roxburgh anoectochilus terminal bud is solved the problems, such as, seedling cost is significantly
Reduce, so as to improve economic benefit.
Specific embodiment
The present invention is described in further details below by example, these examples are only used for illustrating the present invention, do not limit
The scope of the present invention processed.
Embodiment 1
A kind of roxburgh anoectochilus terminal bud field rapid propagation method, comprises the following steps：
（1）Maternal plant preserves
Perennial root is preserved：Overground part plant is harvested before plant bolting is bloomed, retains one stipes of basal part of stem, after being harvested after harvesting,
Immediately reservation basal part of stem is sprayed with 30% agricultural streptomycin, 4000~5000 times of liquid, 2-3 hypophosphorous acid potassium dihydrogen 1000 is sprayed after 1 week
Times liquid, 7-10d is sprayed 1 time.After harvesting 2 weeks, perennial root material is sprayed with 45% amino acid, 500 times of liquid, sprayed within 7-10 days 1 time, carried out
Keep a full stand of seedings management.
（2）Matrix prepares
Using new China fir wood chip as cultivation matrix；
Matrix is sterilized：Using preposition in Exposure to Sunlight under sunlight, 5 kilograms of white limes are added to mix thoroughly in subsequent every 100 kilograms of cultivation matrixes,
Matrix is mixed thoroughly with clear water, it is desirable to which without wet, stromal thickness is 8~10cm to profit；
（3）Cuttings treatment
Using terminal bud cuttings, branch terminal bud defoliation retains to 2-3 piece leaves；
（4）Cuttage dense planting
Terminal bud dense planting：Soaked terminal bud cuttings is inserted directly into the matrix for preparing, and is planted by seeding row spacing 5cm × 5cm kinds, each kind
Plant square plate to transplant 140~150 plants, the depth of planting is 1cm or so；
（5）Planting management
Overwinter management：More than 10 DEG C of control temperature, initial intensity of illumination 1500-2000lux, air humidity 50%~70% or so；
Promote germ tube reason：In month in next year 2-3, air humidity more than 70%, temperature is controlled to spray within 7~10 days 1 N at 20~28 DEG C:
P:K（30:10:10）Nutrient solution；
Daily management：Control air humidity more than 70%, temperature sprays 1 N for 7~10 days at 20~28 DEG C:P:K（20:20:20）
Nutrient solution；
Prevention and elimination of disease and pests：Greenhouse all around goes up fly net, using sugar-vinegar liquid trap and kill black cutworm, using bacterium, fungi, virus,
The biological pesticides such as plant leaching liquor immersion, antibiotic kill disease pest；
（6）Harvesting
Harvesting standard：After cuttings plant 7-8 months, plant 10~more than 15cm high has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is long 3~3.5 centimetres, and blade back is in aubergine, and the blade face network of vein substantially, can harvesting when stem is in green；
Collecting method：Complete stool is left and taken as next year propagating materials in part, remaining whole locality upper bit, retains basal part of stem one
Stipes, is sprayed with streptomysin and continues to employ maternal plant immediately after harvesting, potassium dihydrogen phosphate is sprayed after one week and amino acid is kept a full stand of seedings and promoted
Germ tube is managed.
Embodiment 2
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, comprises the following steps：
（1）Maternal plant preserves
Complete stool preserves：It is September to October in the harvest season, retains small part plant and do not harvest, gives over to March in next year breeding material
Material；
（2）Matrix prepares
Peat soil：River sand：Perlite=10：1：2 proportionings carry out preparation cultivation matrix；
Matrix is sterilized：Added using preposition in Exposure to Sunlight under sunlight, in subsequent every 100 kilograms of cultivation matrixes 7.5 kilograms of white limes or
Appropriate 50% thiophanate methyl or 30% streptomysin is mixed thoroughly, and matrix requirement profit is without wet, water content control to 70% or so；
Medium in tray：Matrix is installed with square planting tray, stromal thickness is 8~10cm；
（3）Cuttings treatment
To be 3~4cm stem sections with axillary bud branch sheared length, remove blade, be soaked with 4000~5000 times of agricultural streptomycins
1min, then soak 10~15min with 100ppm IBA and IAA；
（4）Cuttage dense planting
Soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation square plate plant 80~90
Individual stem section, the depth of planting is 3-5mm, and matrix 2-3mm is covered on top layer
（5）Planting management
Routine Management：Sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, it is in good time according to weather condition after inserting
Water spray moisturizing, controls air humidity more than 70%, and soil humidity maintains 70~75%, and temperature is in 20~28 DEG C, intensity of illumination control
In 1000-1500lux；
Promote germ tube reason：Start within 1 week after plant to spray 1 N:P:K（30:10:10）Nutrient solution, after sprouting sprouting, sprays for 7~10 days
1 N:P:K（20:20:20）Nutrient solution, gradually strengthens illumination, and intensity control is in 2000-3000lux.
Prevention and elimination of disease and pests：Greenhouse all around goes up fly net, black cutworm is trapped and killed using sugar-vinegar liquid, using bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
（6）Harvesting
Harvesting standard：After cuttings plant 7-8 months, plant 10~more than 15cm high has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is long 3~3.5 centimetres, and blade back is in aubergine, and the blade face network of vein substantially, can harvesting when stem is in green；
Collecting method：Complete stool is left and taken as next year propagating materials in part, remaining whole locality upper bit, retains basal part of stem one
Stipes, is sprayed with streptomysin and continues to employ maternal plant immediately after harvesting, potassium dihydrogen phosphate is sprayed after one week and amino acid is kept a full stand of seedings and promoted
Germ tube is managed.
Embodiment 3
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, comprises the following steps：
（1）Maternal plant preserves
Complete stool preserves：It is September to October in the harvest season, retains small part plant and do not harvest, gives over to March in next year breeding material
Material；
（2）Matrix prepares
Peat soil：River sand：Perlite=10：1：2 proportionings carry out preparation cultivation matrix；
Matrix is sterilized：Using preposition in Exposure to Sunlight under sunlight, 5~7.5 kilograms of ripe stones are added in subsequent every 100 kilograms of cultivation matrixes
Ash or 50% appropriate thiophanate methyl or 30% streptomysin are mixed thoroughly, and matrix requirement profit is without wet, water content control to 70% left side
It is right；
Medium in tray：Matrix is installed with square planting tray, stromal thickness is 8~10cm；
（3）Cuttings treatment
Retain branch terminal bud defoliation to top 2-3 piece leaves, soak 1min with 4000~5000 times of agricultural streptomycins, then use
100ppm IBA and IAA soak 10~15min；
（4）Cuttage dense planting
Soaked terminal bud cuttings is inserted directly into the matrix for preparing, and is planted by seeding row spacing 5cm × 5cm kinds, and each plantation square plate is planted
140~150 plants are planted, the depth of planting is 1cm or so；
（5）Planting management
Routine Management：Sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid, it is in good time according to weather condition after inserting
Water spray moisturizing, controls air humidity more than 70%, and soil humidity maintains 70~75%, and temperature is in 20~28 DEG C, intensity of illumination control
In 1500-2000lux；
Promoting root growth is managed：Start within 1 week after plant to spray 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 times, spray within 7-10 days once, sprout
After new root, 1 N is sprayed within 7~10 days:P:K（20:20:20）Nutrient solution, gradually strengthens illumination, and intensity control is left in 3000lux
It is right；
Prevention and elimination of disease and pests：Greenhouse all around goes up fly net, and black cutworm is trapped and killed using sugar-vinegar liquid；
（6）Harvesting
Harvesting standard：After cuttings plant 7-8 months, plant 10~more than 15cm high has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is long 3~3.5 centimetres, and blade back is in aubergine, and the blade face network of vein substantially, can harvesting when stem is in green；
Collecting method：Complete stool is left and taken as next year propagating materials in part, remaining whole locality upper bit, retains basal part of stem one
Stipes, is sprayed with streptomysin and continues to employ maternal plant immediately after harvesting, potassium dihydrogen phosphate is sprayed after one week and amino acid is kept a full stand of seedings and promoted
Germ tube is managed.
Claims (8)
1. a kind of roxburgh anoectochilus terminal bud field rapid propagation method, it is characterised in that：Comprise the following steps：
（1）Maternal plant preserves
Complete stool preserves：It is September to October in the harvest season, retains small part plant and do not harvest, gives over to March in next year breeding material
Material；
Perennial root is preserved：Overground part plant is harvested before plant bolting is bloomed, retains one stipes of basal part of stem, chain is used immediately after harvesting
Mycin is sprayed continues to employ maternal plant, and potassium dihydrogen phosphate and amino acid are sprayed after one week carries out management of keeping a full stand of seedings；
（2）Matrix prepares
Substrate composition：Peat soil, river sand, perlite are according to 10：1：2 proportionings carry out preparation cultivation matrix, or use new China fir
Wood chip；
Matrix is sterilized：Using preposition in Exposure to Sunlight under sunlight, 5~7.5 kilograms of white limes are added in subsequent every 100 kilograms of cultivation matrixes
Or 50% thiophanate methyl or 30% streptomysin mix thoroughly, matrix is mixed thoroughly with clear water, it is desirable to which without wet, stromal thickness is 8~10cm to profit；
（3）Cuttings treatment
Terminal bud cuttings：Retain branch terminal bud defoliation to 2-3 piece leaves；
Stem section cuttings：To be 3~4cm stem sections with axillary bud branch sheared length, remove blade；
Pretreatment：The terminal bud and stem section cuttings sheared soak 30-60s with 4000~5000 times of streptomysins, then use
100ppm indolebutyric acids IBA and indole-3-acetic acid IAA soaks 5~15min；
（4）Cuttage dense planting
Terminal bud dense planting：Soaked terminal bud cuttings is inserted directly into the matrix for preparing, and is planted by seeding row spacing 5cm × 5cm kinds, each kind
Plant square plate to transplant 140~150 plants, the depth of planting is 1cm；
Stem section cuttage：Soaked stem section cuttings is horizontally placed in matrix, and planting density is 5cm × 10cm, each plantation square plate
80~90 stem sections of plant, the depth of planting is 3-5mm, and matrix 2-3mm is covered on top layer；
（5）Planting management
Routine Management：Sprayed with 50% thiophanate methyl wettable powder, 800~1000 times of liquid after cultivation, according to weather condition after inserting
Water spray moisturizing in good time, controls air humidity more than 70%, and soil humidity maintains 70~75%, and temperature is at 20~27 DEG C, and illumination is strong
Degree 2000-3000lux, sprays 1 nutrient solution for 7~10 days；
Prevention and elimination of disease and pests：Greenhouse all around goes up fly net, and black cutworm is trapped and killed using sugar-vinegar liquid, or using bacterium, fungi, disease
The biological pesticides such as poison, plant leaching liquor immersion, antibiotic kill disease pest；
（6）Harvesting
Harvesting standard：After cuttings plant 7-8 months, plant 10~more than 15cm high has 5~6 leaves, 2~2.5cm of leaf width,
Leaf is long 3~3.5 centimetres, and blade back is in aubergine, and the blade face network of vein substantially, can harvesting when stem is in green；
Collecting method：Complete stool is left and taken as next year propagating materials in part, remaining whole locality upper bit, retains basal part of stem one
Stipes, is sprayed with streptomysin and continues to employ maternal plant immediately after harvesting, potassium dihydrogen phosphate is sprayed after one week and amino acid is kept a full stand of seedings and promoted
Germ tube is managed.
2. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（1）Described
Complete stool is preserved and perennial root is preserved, and its overground part is cut and makees cuttings cultivation new talent by March in next year, and underground part is bred as perennial root.
3. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（3）And step
（4）Carried out in March in next year.
4. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（4）Described
Cuttings treatment is that overground part branch is divided into two kinds of cuttage materials of stem apex and stem section to carry out classification cultivation management.
5. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（5）Described
Plant includes terminal bud cuttings and stem section cuttings.
6. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Described terminal bud cuttings
It is to start within 1 week after plant to spray 1000 times of 1 hypophosphorous acid potassium dihydrogen liquid 2-3 times, sprays within 7-10 days once, after sprouting new root, 7~
Spray within 10 days 1 N:P:K ratios are 20:20:20 nutrient solution, gradually strengthens illumination, and intensity control is in 3000lux or so.
7. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Described stem section cuttings
Planting system is that cuttings is placed horizontally in matrix；Start within 1 week after plant to spray 1 N:P:K ratios are 30:10:10 nutrition
Liquid, after sprouting sprouting, sprays 1 N for 7~10 days:P:K ratios are 20:20:20 nutrient solution, gradually strengthens illumination, and intensity control exists
2000-3000lux。
8. a kind of roxburgh anoectochilus terminal bud field rapid propagation method according to claim 1, it is characterised in that：Step（6）Described
After harvesting, complete stool sprays 45% amino acid, 500 times of liquid are kept a full stand of seedings every 7-10 days, perennial root maternal plant is sprayed every 7-10 days
Applying 1000 times of liquid of potassium dihydrogen phosphate carries out promoting germ tube reason.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108200863A (en) * | 2017-12-13 | 2018-06-26 | 福建省热带作物科学研究所 | A kind of culture medium and its application for being used to cultivate hectolitre pine |
| CN108718991A (en) * | 2018-03-30 | 2018-11-02 | 陆川县凯悦鑫种养专业合作社 | A kind of cultural method of gynura bicolor |
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