CN105900622A - Twig cutting seedling breeding method for lonicera macranthoides - Google Patents
Twig cutting seedling breeding method for lonicera macranthoides Download PDFInfo
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- CN105900622A CN105900622A CN201610228412.XA CN201610228412A CN105900622A CN 105900622 A CN105900622 A CN 105900622A CN 201610228412 A CN201610228412 A CN 201610228412A CN 105900622 A CN105900622 A CN 105900622A
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- Prior art keywords
- cup
- cutting
- plastic bag
- substrate
- largeflower
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a twig cutting seedling breeding method for lonicera macranthoides. The method comprises the steps that (1) a disposable plastic cup and a disposable plastic bag are prepared, a hole is cut in the bottom of the disposable plastic cup, and a hole is cut in the plastic bag for subsequent use; (2) a matrix is put into the disposable plastic cup; (3) the opening of the disposable cup is wrapped by the plastic bag and then firmly tied, and the hole of the plastic bag is located at the center of the opening of the disposable plastic cup; and (4) a lonicera macranthoides cutting is inserted via the hole of the plastic bag into the matrix for seedling breeding. The method disclosed by the invention effectively solves the current problem of cutting rotting during twig cutting rooting of old lonicera macranthoides and can be applied in the large-area twig cutting seedling breeding of the lonicera macranthoides. Generalization and application of the method disclosed by the invention can greatly enhance a supply capability of lonicera macranthoides improved seedlings and can obtain huge economic benefits.
Description
Technical field
The invention belongs to plant biotechnology field, relate to a kind of largeflower-like honeysuckle flower epicormic branch cutting method.
Background technology
Largeflower-like honeysuckle flower is the principal item of south commodity Flos Lonicerae cultivation, main containing chlorogenic acid, luteoloside, saponin etc.
Want composition, there is heat-clearing and toxic substances removing, effect of cool breeze heat radiation, can enhancing human body immunity power, treatment malignant tumor etc. is had solely
Specially good effect fruit.Wide adaptability, strong stress resistance, cold-resistant, drought-enduring, barren-resistant, disease and insect resistance, soil is required the tightest.Closely
Nian Lai, along with being continuously increased of the market demand, has selected a collection of improved Varieties, such as ' Jin Cuilei ', ' white
Cloud ', ' Hua Yao is late-maturing ', ' dragon flower ' etc., fast breeding new varieties nursery stock is very urgent.Largeflower-like honeysuckle flower is many at present
Using grafting method breeding nursery stock, there is breeding coefficient little, growing-seedling period is long, the defect that variety deterioration is fast；Also there is few portion
Dividing and use tissue culture quick breeding nursery stock, but the method operation is numerous and diverse, production cost is too high, is difficult to spread in production
Application.Use softwood cutting propagation nursery stock, the excellent hereditary character of maternal plant can be preserved, growing-seedling period can be shortened again, significantly
Degree reduces production cost, is a kind of suitable fast breeding method.
Publication number CN 103314748 A " method for culturing seedlings of a kind of breeding Flos Lonicerae " patent, the inventive features of applicant exists
In nursery step be select breeding, HORMONE TREATMENT, carry out cuttage using perlite as substrate, take root after transplant to land for growing field crops refine
Seedling etc.；Publication number CN 103947402 A " cottage breeding method of a kind of Flos Lonicerae " and publication number CN 102369835 A
" a kind of vegetative propagation method of honeysuckle " patent, its feature is all that cuttage branch prepares, seedbed prepares, cuttage branch processes
With seedbed management etc..The research of largeflower-like honeysuckle flower cutting propagation is more, but is confined to general Technique on Cuttage Propagation, research contents
Relating generally to the impact on cuttage survival rate such as cuttage period, cutting medium, root-growing agent process, cuttage vessel mostly is cuttage
Dish, nutritive cube, non-woven fabrics nutrient bag etc..It was found that largeflower-like honeysuckle flower to cultivate certain time limit, physiological age bigger than normal
Time, the rattan of germination and growth has hollow more, hot and humid under the conditions of, cutting is easily rotted, and when moisture content deficiency, affects again
Cutting root of hair, reduces survival rate.Therefore, continue research and development one and both can guarantee that cuttage rooting, be avoided that again and draw because humidity is excessive
Play cutting to rot, the method improving cuttage survival rate.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, it is provided that a kind of largeflower-like honeysuckle flower epicormic branch cutting method.The method can control
Largeflower-like honeysuckle flower epicormic branch cuttage cutting rotting rate.
Described largeflower-like honeysuckle flower epicormic branch cutting method comprises the steps:
(1) disposal plastic cup and plastic bag are prepared, by perforate at the bottom of disposal plastic cup cup, by plastic bag perforate, standby；
(2) in disposal plastic cup, substrate is loaded；
(3) plastic bag encasing disposal plastic cup rim of a cup and pricks solid, the perforate of described plastic bag is in disposal plastic cup
Rim of a cup center；
(4) by largeflower-like honeysuckle flower cutting from the tapping cuttage of plastic bag to nursery substrate.
Wherein, perforate number at the bottom of described disposal plastic cup cup is 3, and opening diameter is 3 6mm；Described plastic bag perforate
Number is 1, and opening diameter is 2 3cm.Described substrate is by mud stone soil and perlite 1:(1.5 2.5 by volume),
It is preferably 1:2 composition.Described substrate volume is the 3/4 4/5 of disposal plastic cup volume.Step (2) is with dilution 800
Concentration of volume percent after Bei be 75% cymoxanil mancozeb to substrate sterilize after again substrate is loaded in disposal plastic cup, dress
Require when entering container that substrate is held tightly, hands pine i.e. bulk.Step (3) is prick admittedly with rubber band.Step in (4) is
After KIBA quickly dipped largeflower-like honeysuckle flower cutting base portion 10-30s with 1000～2000mg/L again by largeflower-like honeysuckle flower cutting cuttage extremely
In substrate.
The material specification used in the present invention is as follows:
Disposal plastic cup cup high 7～9cm, rim of a cup 7～8cm, at the bottom of cup 5～6cm
Polyethylene plastic bag thickness 1.5～2.0, diameter 18～20cm
Rubber band diameter 5～6cm.
Preferably,
Disposal plastic cup cup high 7～8cm, rim of a cup 7～8cm, at the bottom of cup 5～6cm
Polyethylene plastic bag thickness 1.5～1.8, diameter 18～19cm
Rubber band diameter 5～5.5cm.
The resistance of pathogen, after parent cuts off, is weakened by largeflower-like honeysuckle flower cutting, under hot and humid, and cutting base portion
Easily infected by pathogen, before not yet forming callus, the most produce pathological changes and rot, and physiological age is the biggest,
Being more difficult to form callus, cutting base portion rots the fastest, and result is that last whole cutting is rotted, it is impossible to regenerated root；If but
Moisture content deficiency affects again cutting root of hair, also can reduce survival rate.The method using the present invention carries out cottage propagation, in interval
Under spray condition, it is to avoid leaves water loss is wilted or comes off, and protects resting bud further, under the photosynthesis of blade,
Be conducive to the accumulation of nutrient substance, stimulate and promote largeflower-like honeysuckle flower cuttage root-taking；Meanwhile, have because of disposal plastic cup
Plastic bag sealing, only few part water smoke can enter in cup, and the cutting in cup can be made to keep certain humidity, the most not
Cutting can be induced to take root smoothly because excess moisture causes cutting to rot, improve adventitious root growth rate and root system is close
Degree, thus improve cuttage survival rate.
The practice result result of the present invention shows, cutting rotting rate reduction by 60～80%, survival rate 85～95%, large area
During application, rooting percent and cuttage survival rate significantly improve, and average survival reaches 92%, improve 60%～80% on year-on-year basis.Use
The inventive method, after epicormic branch cuttage 12～16 days i.e. start to take root, the cuttage rooting of more than 90% after 30 days, radical 3～5,
Root length 2～3cm.
The present invention efficiently solves the problem that in current above the average age for marriage largeflower-like honeysuckle flower epicormic branch cuttage rooting process, cutting rots, can be big
Area is applied to largeflower-like honeysuckle flower epicormic branch cutting.The popularization and application of the present invention, will be greatly improved largeflower-like honeysuckle flower breeding kind
The deliverability of Seedling, receives huge economic benefit.
Detailed description of the invention
Embodiment 1
The each raw material of container and specification be:
Disposal plastic cup (the high 8cm of cup, rim of a cup 7cm, 5cm at the bottom of cup), polyethylene plastic bag (thickness 1.5, diameter
19cm), rubber band (diameter 5.5cm).
Described largeflower-like honeysuckle flower epicormic branch cutting method comprises the steps:
(1) disposal plastic cup and plastic bag are prepared, by perforate at the bottom of disposal plastic cup cup, by plastic bag perforate, standby；
(2) in disposal plastic cup, substrate is loaded；
(3) plastic bag encasing disposal plastic cup rim of a cup and pricks with rubber band solid, the perforate of described plastic bag is in once
Property plastic cup rim of a cup center；It is positioned on the seedbed of full exposure automatic intermittent spraying device standby；
(4) clip 3 kinds above the average age for marriage largeflower-like honeysuckle flower semi-lignified twig makees cutting, and cutting is cut into 8～10cm length, top tape
2 leaves, every leaf only stays a half vane, and lower end is cut into inclined-plane, and smooth cut is without tear；With 1000～2000mg/L
KIBA quickly dipped largeflower-like honeysuckle flower cutting base portion 10-30s, by largeflower-like honeysuckle flower cutting cuttage to nursery in substrate after 10min.
Wherein, perforate number at the bottom of described disposal plastic cup cup is 3, and opening diameter is 3 6mm；Described plastic bag perforate
Number is 1, and opening diameter is 2 3cm.Described substrate is made up of mud stone soil and perlite 1:2 by volume.Described substrate
Volume is the 3/4 4/5 of disposal plastic cup volume.Step (2) is be with the concentration of volume percent after dilution 800 times
Substrate is loaded in disposal plastic cup after sterilizing substrate by the cymoxanil mancozeb of 75% again, requires that substrate is held when loading container
Tightly, hands pine i.e. bulk.
Investigation in 40 days after cuttage, result is following (table 1):
Table 1
Meanwhile, in the case of other condition is identical, 4 kind largeflower-like honeysuckle flowers listed to table 1 without the inventive method,
But cuttage in common cuttage dish or nutritive cube to show contrast, result following (table 2):
Table 2
Embodiment 2
Material specification is: disposal plastic cup (the high 7.5cm of cup, rim of a cup 7cm, 5cm at the bottom of cup), and polyethylene plastic bag is (thick
Spend 1.8, diameter 18cm), rubber band (diameter 5cm).
Cuttage and seedling culture method is with embodiment 1.Tloig-cutlage after processing, in the disposal plastic cup filling substrate, is carried out
Management nursery.Cutting rotting rate is respectively that the emerald green flower bud 9.2% of gold, white clouds 7.1%, Hua Yao be late-maturing 12.2%, dragon spends 6.6%, cuttage
Survival rate is respectively that the emerald green flower bud 91.8% of gold, white clouds 92.9%, Hua Yao be late-maturing 87.8%, dragon spends 93.4%.
Embodiment 3
Material specification is: disposal plastic cup (the high 9cm of cup, rim of a cup 8cm, 6cm at the bottom of cup), polyethylene plastic bag (thickness
1.5, diameter 19cm), rubber band (diameter 6cm).
Cuttage and seedling culture method is with embodiment 1.Tloig-cutlage after processing, in the disposable plastic filling substrate, carries out pipe
Reason nursery.Cutting rotting rate is respectively that the emerald green flower bud 8.4% of gold, white clouds 7.0%, Hua Yao be late-maturing 5.5%, dragon spends 6.7%, cuttage
Survival rate is respectively that the emerald green flower bud 91.6% of gold, white clouds 93.0%, Hua Yao be late-maturing 94.5%, dragon spends 93.3%.
Claims (7)
1. a largeflower-like honeysuckle flower epicormic branch cutting method, it is characterised in that described method comprises the steps:
(1) disposal plastic cup and plastic bag are prepared, by perforate at the bottom of disposal plastic cup cup, by plastic bag perforate, standby；
(2) in disposal plastic cup, substrate is loaded；
(3) plastic bag encasing disposal plastic cup rim of a cup and pricks solid, the perforate of described plastic bag is in disposal plastic cup rim of a cup
Center；
(4) by largeflower-like honeysuckle flower cutting from the tapping cuttage of plastic bag to nursery substrate.
2. the method for claim 1, it is characterised in that perforate number at the bottom of described disposal plastic cup cup is 3, perforate
A diameter of 3 6mm；Described plastic bag perforate number is 1, and opening diameter is 2 3cm.
3. the method for claim 1, it is characterised in that described substrate is by mud stone soil and perlite 1:(1.5 by volume
2.5) composition.
4. method as claimed in claim 3, it is characterised in that described substrate is made up of mud stone soil and perlite 1:2 by volume.
5. the method for claim 1, it is characterised in that described substrate volume is the 3/4 4/5 of disposal plastic cup volume.
6. the method for claim 1, it is characterised in that be dense by the percent by volume after dilution 800 times in step (2)
Degree be 75% cymoxanil mancozeb to substrate sterilize after again substrate is loaded in disposal plastic cup.
7. the method for claim 1, it is characterised in that be that the KIBA with 1000～2000mg/L is quickly dipped in step (4)
Again by largeflower-like honeysuckle flower cutting cuttage to substrate after largeflower-like honeysuckle flower cutting base portion 10-30s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610228412.XA CN105900622A (en) | 2016-04-13 | 2016-04-13 | Twig cutting seedling breeding method for lonicera macranthoides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610228412.XA CN105900622A (en) | 2016-04-13 | 2016-04-13 | Twig cutting seedling breeding method for lonicera macranthoides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105900622A true CN105900622A (en) | 2016-08-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610228412.XA Pending CN105900622A (en) | 2016-04-13 | 2016-04-13 | Twig cutting seedling breeding method for lonicera macranthoides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706796B (en) * | 2016-03-04 | 2018-08-28 | 重庆市中药研究院 | A kind of method of integrated control largeflower-like honeysuckle flower giant mealy bug |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820585A (en) * | 2006-03-23 | 2006-08-23 | 刘海涛 | High efficiency breeding technology for lonicera macranthoides honeysuckle |
| KR20120133787A (en) * | 2011-06-01 | 2012-12-11 | 조원제 | Method for artificial cultivation of dodder |
| CN102907445A (en) * | 2012-11-14 | 2013-02-06 | 湖南省林业科学院 | Pesticide and method for controlling rate of fallen leaves of largeflower-like honeysuckle flower twig cutting seedling |
| CN103299854A (en) * | 2012-03-12 | 2013-09-18 | 吴晓丽 | Potted plant substrate bag |
| CN104641998A (en) * | 2015-02-05 | 2015-05-27 | 江苏科技大学 | Heating hollow nutrient bag seedling culturing box and application thereof |
| CN104855104A (en) * | 2015-05-22 | 2015-08-26 | 湖南省核农学与航天育种研究所 | Environment-friendly no-tillage flower cultivation method |
-
2016
- 2016-04-13 CN CN201610228412.XA patent/CN105900622A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820585A (en) * | 2006-03-23 | 2006-08-23 | 刘海涛 | High efficiency breeding technology for lonicera macranthoides honeysuckle |
| KR20120133787A (en) * | 2011-06-01 | 2012-12-11 | 조원제 | Method for artificial cultivation of dodder |
| CN103299854A (en) * | 2012-03-12 | 2013-09-18 | 吴晓丽 | Potted plant substrate bag |
| CN102907445A (en) * | 2012-11-14 | 2013-02-06 | 湖南省林业科学院 | Pesticide and method for controlling rate of fallen leaves of largeflower-like honeysuckle flower twig cutting seedling |
| CN104641998A (en) * | 2015-02-05 | 2015-05-27 | 江苏科技大学 | Heating hollow nutrient bag seedling culturing box and application thereof |
| CN104855104A (en) * | 2015-05-22 | 2015-08-26 | 湖南省核农学与航天育种研究所 | Environment-friendly no-tillage flower cultivation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706796B (en) * | 2016-03-04 | 2018-08-28 | 重庆市中药研究院 | A kind of method of integrated control largeflower-like honeysuckle flower giant mealy bug |
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Application publication date: 20160831 |
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