CN113678736A - Tissue culture rapid propagation method of amorphophallus rivieri - Google Patents
Tissue culture rapid propagation method of amorphophallus rivieri Download PDFInfo
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- CN113678736A CN113678736A CN202111178813.6A CN202111178813A CN113678736A CN 113678736 A CN113678736 A CN 113678736A CN 202111178813 A CN202111178813 A CN 202111178813A CN 113678736 A CN113678736 A CN 113678736A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
Abstract
The invention discloses a tissue culture rapid propagation method of amorphophallus konjac, which comprises the steps of explant sterilization, primary culture, tissue induction, differentiation culture, rooting culture, subculture and hardening-seedling transplantation. The tissue culture rapid propagation method of the alocasia cucullata is adopted, the leaves of the alocasia cucullata are used as materials to carry out tissue culture of the alocasia cucullata, mass propagation of the alocasia cucullata can be carried out through the tissue culture, the growth and propagation period is short, the method is not influenced by external factors such as environmental seasons and the like, the production plan can be adjusted according to market conditions, and the economic value is high.
Description
Technical Field
The invention relates to the technical field of tissue culture and propagation of sample plants, in particular to a tissue culture and rapid propagation method of amorphophallus rivieri.
Background
The Oriental Colocasiana (Caladium bicolor (air.) Vent.) of Araceae belongs to perennial evergreen herbaceous plant. Basal leaf shield-shaped arrow or heart-shaped, beautiful color and extremely many varieties; the spathe is green, the upper part is green and white, and the spathe is in a shell shape; panicle inflorescence. The underground part has the swelling tuber, oblate sphere, toxicity and paralysis of the larynx and the tongue after being eaten by mistake. Native to the river basin of amazon south america, fond of high temperature, high humidity and semi-yin environment, and do not resist low temperature and frost and snow, and require loose soil, fertility and good drainage.
The leaves are beautiful in color and many in varieties, and are suitable for greenhouse cultivation and appreciation, summer is the main appreciation period, and the leaves are colorful. Leaves are disordered when entering autumn, and the leaves are withered and yellow in winter, enter a dormant period, and begin to germinate and grow again when the spring is late and the summer is early. And 5-9 months is its vigorous growth period.
The colocasia esculenta can be bred by a method of dividing plants or balls. Plants can be divided all the year round, but the plants are preferably divided after dormancy in winter and before germination of leaves in spring. Cutting the pellets around the mother ball with a blade, and planting after drying in the shade for 1 day; the mother ball can also be cut into blocks, each block is provided with a bud eye, the mother ball is planted after being dried in the shade for 1 day, the section is smooth, the wound healing, rooting and seedling formation are facilitated, and the ball division method is slow, not beneficial to mass propagation and expensive in plant price, so that the tissue culture method is adopted for rapid propagation.
Disclosure of Invention
The invention aims to provide a tissue culture rapid propagation method of amorphophallus konjac, which adopts different culture media at each stage, so that the excellent characters of plants can be preserved, the time and the cost of in-vitro culture are shortened, and the survival rate of seedlings is improved.
In order to realize the aim, the invention provides a tissue culture rapid propagation method of amorphophallus konjac, which comprises the following steps:
preparation of culture Medium
1) Primary culture medium: taking an improved 1/2MS culture medium as a basic culture medium, adding 0.1-1.5 mg/L of 6-furfuryl adenine, 5g/L of coconut juice, 10g/L of banana juice, 50g/L of cane sugar, 7g/L of agar and 5.8-6.0 of PH;
2) tissue induction medium: taking an improved MS culture medium as a basic culture medium, adding 1-2 mg/L of 6-benzylaminopurine, 0.1-1 mg/L of naphthylacetic acid, 50g/L of cane sugar, 7g/L of agar and 5.8-6.0 of PH;
3) differentiation medium: taking an improved MS culture medium as a basic culture medium, adding 1-2 mg/L of 6-benzylaminopurine, 0.5-1.5 mg/L of zeatin, 0.1-2 mg/L of thidiazuron, 0.1-1 mg/L of naphthylacetic acid, 80g/L of cane sugar, 7g/L of agar and 5.8-6.2 of PH;
4) rooting culture medium: taking an improved MS culture medium as a basic culture medium, adding 0.1-1 mg/L of naphthylacetic acid, 0.1-1 mg/L of IBA, 0.5% of active carbon, 80g/L of sucrose, 7g/L of agar and 5.8-6.2 of PH;
5) subculture medium: taking an improved WPM culture medium as a basic culture medium, adding 0.5-1 mg/L of naphthylacetic acid, 1% of activated carbon, 80g/L of sucrose, 7g/L of agar and 5.8-6.2 of PH;
cultivation of tissue culture seedlings of (II) floret taro
1) Selecting explants: selecting green, tender and complete leaves on a dasheen mosaic plant which has no plant diseases and insect pests and good growth vigor as an explant;
2) and (3) explant sterilization: washing the explant prepared in the step 1) with clear water for 2-5 minutes, rubbing and soaking the explant with detergent for 1-3 minutes, washing with clear water for 3-4 times, transferring the explant to a clean bench, sterilizing with 75% alcohol for 10-15 seconds, treating with 50% sodium hypochlorite for 5-7 minutes, washing with sterile water for 4-6 times, and then sucking out surface moisture with disinfection filter paper and placing the surface moisture in a culture dish for later use;
3) primary culture: cutting the explants sterilized in the step 2) into squares, respectively inoculating and spreading on a primary culture medium, wherein the culture conditions are as follows: the culture temperature is 25-28 ℃, the culture humidity is 50%, the illumination intensity is 1500-;
4) tissue induction: transferring the primary flower leaf taro tissue culture seedlings obtained after the primary culture for four or five days in the step 3) into a tissue induction culture medium for continuous culture, wherein the culture conditions are as follows: the culture temperature is 25-27 ℃, the culture humidity is 45%, the illumination intensity is 1500-2000 lx, and the illumination time is 14 h/d;
5) differentiation culture: after one week of tissue induction culture in the step 4), growing callus on the periphery of the leaves, transferring the leaves with the callus on the periphery to a differentiation culture medium for continuous culture, and after 20-25 days, growing adventitious buds on the periphery of the leaves under the culture conditions: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
6) rooting culture: after the differentiation culture in the step 5), selecting more than 1cm of adventitious buds to transfer into a rooting culture medium for rooting and strengthening seedling culture, wherein the culture conditions are as follows: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx
7) Subculturing: after the rooting culture is carried out for 25-30 d in the step 6), dividing the rooting tissue culture seedling of the florasulam into 1-2 side buds and a stem section in a super clean workbench when the rooting tissue culture seedling of the florasulam grows to 4-10 cm high, and transferring the stem section to a subculture medium for rapid propagation under the culture conditions: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
8) transplanting and hardening seedlings: carrying out subculture for 30-40 d in the step 6) to obtain subculture tissue culture seedlings, opening a bottle cap and hardening the seedlings for 1-2 days when the subculture tissue culture seedlings grow to 4-5 cm high, 3-5 roots and 2-3 leaves; then taking out the tissue culture seedlings of the dasheen hybrida, cleaning the culture medium at the root by clear water, transplanting the dasheen hybrida into a plug tray filled with the culture medium, and covering a seedling hardening cover; the culture medium is sterilized at high temperature and high pressure, then 20% of perlite and 30% of vermiculite are mixed, the humidity and the illumination are kept, and water is sprayed once in the morning and at night. The culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 70%, and the illumination intensity is 1500-2000 lx.
Preferably, the formula of the modified 1/2MS culture medium is as follows: 1020mg of potassium nitrate, 123mg of calcium chloride, 224mg of magnesium sulfate, 122mg of sodium dihydrogen phosphate, 137mg of ammonium sulfate, 0.68mg of potassium iodide, 3.55mg of boric acid, 100mg of inositol, 7mg of manganese sulfate, 2.45mg of zinc sulfate, 0.011mg of copper sulfate, 0.013mg of cobalt chloride, 23.4mg of disodium edetate, 24.4mg of ferrous sulfate, 0.4mg of sodium molybdate, 3mg of nicotinic acid, 6mg of pyridoxine hydrochloride and 6mg of thiamine hydrochloride.
Preferably, the formula of the modified MS culture medium is as follows: 1020mg of potassium nitrate, 123mg of calcium chloride, 224mg of magnesium sulfate, 122mg of sodium dihydrogen phosphate, 137mg of ammonium sulfate, 0.68mg of potassium iodide, 3.55mg of boric acid, 100mg of inositol, 7mg of manganese sulfate, 2.45mg of zinc sulfate, 0.011mg of copper sulfate, 0.013mg of cobalt chloride, 23.4mg of disodium edetate, 24.4mg of ferrous sulfate, 0.4mg of sodium molybdate, 3mg of nicotinic acid, 6mg of pyridoxine hydrochloride and 6m of thiamine hydrochloride.
Preferably, the formula of the improved WPM culture medium is as follows: 120mg of calcium chloride, 1500mg of potassium nitrate, 100mg of magnesium sulfate, 120mg of ammonium sulfate, 23.4mg of ferrous sulfate, 135mg of sodium dihydrogen phosphate, 10mg of manganese sulfate, 10mg of boric acid, 0.57mg of potassium iodide, 2mg of sodium molybdate, 0.025mg of copper sulfate, 0.12mg of cobalt chloride, 1.5mg of zinc sulfate, 27.5mg of disodium ethylenediamine tetraacetate, 5.68mg of thiamine hydrochloride, 100mg of inositol, 3mg of nicotinic acid and 3.45mg of pyridoxine hydrochloride.
The invention has the beneficial effects that:
(1) provides a tissue culture rapid propagation method of the alocasia cucullata, which is a tissue culture method for inducing the regeneration of leaves by utilizing the alocasia cucullata asepsis, and is more rapid and efficient compared with the traditional propagation method.
(2) The leaf disinfection device provided by the invention uses 75% of alcohol for sterilization and 50% of sodium hypochlorite for treatment in the leaf disinfection process, the sterilization efficiency is high, and compared with 0.1% of mercuric chloride for disinfection, the leaf disinfection device is more green and environment-friendly, has small damage to plants and is easy to clean.
(3) The primary culture medium, the tissue induction culture medium and the differentiation culture medium can promote the adventitious bud germination of the colocasia esculenta, and the rooting culture medium can improve the survival rate of the colocasia esculenta sprouts. The regeneration adventitious bud rate of the leaves reaches 98%, the growth of adventitious roots can be promoted, the survival rate of the sprouts reaches 98%, and the contamination rate reaches 0.1%.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
FIG. 1 is a schematic diagram of adventitious bud culture in a tissue culture rapid propagation method of colocasia esculenta;
FIG. 2 is a schematic diagram of rooting culture in a tissue culture rapid propagation method of colocasia esculenta of the present invention;
FIG. 3 is a schematic diagram of seedling formation and cultivation by the tissue culture rapid propagation method of colocasia esculenta.
Detailed Description
The present invention will be further described with reference to examples, in which various chemicals and reagents are commercially available unless otherwise specified.
Examples
The invention provides a tissue culture and rapid propagation method of amorphophallus konjac, which comprises the following steps:
step 1) selection of explants: selecting green, tender and complete leaves on a dasheen mosaic plant which has no plant diseases and insect pests and good growth vigor as an explant;
step 2) explant sterilization: washing the explant prepared in the step 1) with clear water for 2-5 minutes, rubbing and soaking the explant with detergent for 1-3 minutes, washing with clear water for 3-4 times, transferring the explant to a clean bench, sterilizing with 75% alcohol for 10-15 seconds, treating with 50% sodium hypochlorite for 5-7 minutes, washing with sterile water for 4-6 times, and then sucking out surface moisture with disinfection filter paper and placing the surface moisture in a culture dish for later use;
step 3) primary culture: cutting the explants sterilized in the step 2) into small blocks, respectively cutting the sterilized tissues into small blocks, and inoculating the small blocks on a primary culture medium in a spread manner, wherein the culture temperature is 25-28 ℃, the humidity is 50%, the illumination intensity is 1500-;
the primary culture medium is prepared from the following formula: modified 1/2MS + 6-furfuryl acyl purine 0.5mg/L + cane sugar 50g/L + agar 7g/L + pH 5.8-6.0;
step 4) tissue induction: respectively inoculating the tissue culture seedlings of the first generation colocasia esculenta which are cultured for four or five days in the step 3) into a new tissue induction culture medium, and inducing tissue callus;
the tissue induction culture medium is prepared from the following formula: modified MS + 6-benzylaminopurine 1mg/L + naphthylacetic acid 0.2mg/L + sucrose 50g/L, agar 7g/L, and pH 5.8-6.0.
Step 5) differentiation culture: after one week of tissue induction culture in the step 4), callus grows out around the leaves, the callus is inoculated to a differentiation culture medium, the callus is induced to differentiate, and cluster buds are differentiated. The differentiation culture time is 20d, the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
the differentiation medium is prepared by the following formula: modified MS + 6-benzylaminopurine 1.5mg/L + zeatin 0.5mg/L + thidiazuron 0.5mg/L + naphthylacetic acid 0.1mg/L + sucrose 80g/L + agar 7g/L + PH 5.8-6.2.
Step 6) rooting culture: after the differentiation culture in the step 5), respectively inoculating the differentiated adventitious buds with the length of more than 1cm into a rooting culture medium, and carrying out rooting and seedling strengthening culture, wherein the rooting culture time is 25-30 d, the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
the rooting medium is prepared from the following formula: modified MS + naphthylacetic acid 0.1mg/L + IBA 0.5mg/L + active carbon 0.5% + sucrose 80g/L + agar 7g/L + pH 5.8-6.2.
Step 7) subculture: and (3) after the rooting culture is carried out for 25-30 d in the step 6), when the rooting tissue culture seedlings of the tuber taros grow to 4-10 cm high, dividing the rooting tissue culture seedlings of the tuber taros into 1-2 side buds and a stem section in a super clean workbench, and respectively inoculating the stem sections into a subculture medium for rapid propagation. The culture conditions are as follows: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
the subculture medium is prepared from the following formula: modified WPM + naphthylacetic acid 0.5mg/L + active carbon 1% + sucrose 80g/L + agar 7g/L + PH 5.8-6.2.
Step 8), transplanting and hardening seedlings: carrying out subculture for 30-40 d in the step 6) to obtain subculture tissue culture seedlings, opening a bottle cap and hardening the seedlings for 1-2 days when the subculture tissue culture seedlings grow to 4-5 cm high, 3-5 roots and 2-3 leaves; then taking out the tissue culture seedlings of the dasheen hybrida, cleaning the culture medium at the root part with clear water, transplanting the dasheen hybrida into a hole tray filled with the culture medium, and covering a seedling hardening cover. The culture medium is sterilized at high temperature and high pressure, then 20% of perlite and 30% of vermiculite are mixed, the humidity and the illumination are kept, and water is sprayed once in the morning and at night. The culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 70%, and the illumination intensity is 1500-2000 lx;
finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (4)
1. A tissue culture rapid propagation method of amorphophallus konjac is characterized by comprising the following steps:
preparation of culture Medium
1) Primary culture medium: taking an improved 1/2MS culture medium as a basic culture medium, adding 0.1-1.5 mg/L of 6-furfuryl adenine, 5g/L of coconut juice, 10g/L of banana juice, 50g/L of cane sugar, 7g/L of agar and 5.8-6.0 of PH;
2) tissue induction medium: taking an improved MS culture medium as a basic culture medium, adding 1-2 mg/L of 6-benzylaminopurine, 0.1-1 mg/L of naphthylacetic acid, 50g/L of cane sugar, 7g/L of agar and 5.8-6.0 of PH;
3) differentiation medium: taking an improved MS culture medium as a basic culture medium, adding 1-2 mg/L of 6-benzylaminopurine, 0.5-1.5 mg/L of zeatin, 0.1-2 mg/L of thidiazuron, 0.1-1 mg/L of naphthylacetic acid, 80g/L of cane sugar, 7g/L of agar and 5.8-6.2 of PH;
4) rooting culture medium: taking an improved MS culture medium as a basic culture medium, adding 0.1-1 mg/L of naphthylacetic acid, 0.1-1 mg/L of IBA, 0.5% of active carbon, 80g/L of sucrose, 7g/L of agar and 5.8-6.2 of PH;
5) subculture medium: taking an improved WPM culture medium as a basic culture medium, adding 0.5-1 mg/L of naphthylacetic acid, 1% of activated carbon, 80g/L of sucrose, 7g/L of agar and 5.8-6.2 of PH;
cultivation of tissue culture seedlings of (II) floret taro
1) Selecting explants: selecting green, tender and complete leaves on a dasheen mosaic plant which has no plant diseases and insect pests and good growth vigor as an explant;
2) and (3) explant sterilization: washing the explant prepared in the step 1) with clear water for 2-5 minutes, rubbing and soaking the explant with detergent for 1-3 minutes, washing with clear water for 3-4 times, transferring the explant to a clean bench, sterilizing with 75% alcohol for 10-15 seconds, treating with 50% sodium hypochlorite for 5-7 minutes, washing with sterile water for 4-6 times, and then sucking out surface moisture with disinfection filter paper and placing the surface moisture in a culture dish for later use;
3) primary culture: cutting the explants sterilized in the step 2) into squares, respectively inoculating and spreading on a primary culture medium, wherein the culture conditions are as follows: the culture temperature is 25-28 ℃, the culture humidity is 50%, the illumination intensity is 1500-;
4) tissue induction: transferring the primary flower leaf taro tissue culture seedlings obtained after the primary culture for four or five days in the step 3) into a tissue induction culture medium for continuous culture, wherein the culture conditions are as follows: the culture temperature is 25-27 ℃, the culture humidity is 45%, the illumination intensity is 1500-2000 lx, and the illumination time is 14 h/d;
5) differentiation culture: after one week of tissue induction culture in the step 4), growing callus on the periphery of the leaves, transferring the leaves with the callus on the periphery to a differentiation culture medium for continuous culture, and after 20-25 days, growing adventitious buds on the periphery of the leaves under the culture conditions: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
6) rooting culture: after the differentiation culture in the step 5), selecting more than 1cm of adventitious buds to transfer into a rooting culture medium for rooting and strengthening seedling culture, wherein the culture conditions are as follows: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx
7) Subculturing: after the rooting culture is carried out for 25-30 d in the step 6), dividing the rooting tissue culture seedling of the florasulam into 1-2 side buds and a stem section in a super clean workbench when the rooting tissue culture seedling of the florasulam grows to 4-10 cm high, and transferring the stem section to a subculture medium for rapid propagation under the culture conditions: the culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 45%, and the illumination intensity is 1500-2000 lx;
8) transplanting and hardening seedlings: carrying out subculture for 30-40 d in the step 6) to obtain subculture tissue culture seedlings, opening a bottle cap and hardening the seedlings for 1-2 days when the subculture tissue culture seedlings grow to 4-5 cm high, 3-5 roots and 2-3 leaves; then taking out the tissue culture seedlings of the dasheen hybrida, cleaning the culture medium at the root by clear water, transplanting the dasheen hybrida into a plug tray filled with the culture medium, and covering a seedling hardening cover; the culture medium is sterilized at high temperature and high pressure, then 20% of perlite and 30% of vermiculite are mixed, the humidity and the illumination are kept, and water is sprayed once in the morning and at night. The culture temperature is 25-27 ℃, the illumination time is 16h/d, the culture humidity is 70%, and the illumination intensity is 1500-2000 lx.
2. The tissue culture rapid propagation method of amorphophallus konjac according to claim 1, wherein the formula of the improved 1/2MS culture medium is as follows: 1020mg of potassium nitrate, 123mg of calcium chloride, 224mg of magnesium sulfate, 122mg of sodium dihydrogen phosphate, 137mg of ammonium sulfate, 0.68mg of potassium iodide, 3.55mg of boric acid, 100mg of inositol, 7mg of manganese sulfate, 2.45mg of zinc sulfate, 0.011mg of copper sulfate, 0.013mg of cobalt chloride, 23.4mg of disodium edetate, 24.4mg of ferrous sulfate, 0.4mg of sodium molybdate, 3mg of nicotinic acid, 6mg of pyridoxine hydrochloride and 6mg of thiamine hydrochloride.
3. The tissue culture rapid propagation method of colocasia esculenta as claimed in claim 1, wherein the formula of the improved MS culture medium is as follows: 1020mg of potassium nitrate, 123mg of calcium chloride, 224mg of magnesium sulfate, 122mg of sodium dihydrogen phosphate, 137mg of ammonium sulfate, 0.68mg of potassium iodide, 3.55mg of boric acid, 100mg of inositol, 7mg of manganese sulfate, 2.45mg of zinc sulfate, 0.011mg of copper sulfate, 0.013mg of cobalt chloride, 23.4mg of disodium edetate, 24.4mg of ferrous sulfate, 0.4mg of sodium molybdate, 3mg of nicotinic acid, 6mg of pyridoxine hydrochloride and 6m of thiamine hydrochloride.
4. The tissue culture rapid propagation method of colocasia esculenta as claimed in claim 1, wherein the formula of the improved WPM medium is as follows: 120mg of calcium chloride, 1500mg of potassium nitrate, 100mg of magnesium sulfate, 120mg of ammonium sulfate, 23.4mg of ferrous sulfate, 135mg of sodium dihydrogen phosphate, 10mg of manganese sulfate, 10mg of boric acid, 0.57mg of potassium iodide, 2mg of sodium molybdate, 0.025mg of copper sulfate, 0.12mg of cobalt chloride, 1.5mg of zinc sulfate, 27.5mg of disodium ethylenediamine tetraacetate, 5.68mg of thiamine hydrochloride, 100mg of inositol, 3mg of nicotinic acid and 3.45mg of pyridoxine hydrochloride.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114885839A (en) * | 2022-05-11 | 2022-08-12 | 中国科学院华南植物园 | Method for rapidly propagating seedlings of stem of common andrographis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114885839A (en) * | 2022-05-11 | 2022-08-12 | 中国科学院华南植物园 | Method for rapidly propagating seedlings of stem of common andrographis |
| CN114885839B (en) * | 2022-05-11 | 2022-12-30 | 中国科学院华南植物园 | Method for rapidly propagating seedlings of stem of common andrographis |
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