CN110506600A - Utilize the method for sugarcane health seedling ineffective tillering nursery - Google Patents
Utilize the method for sugarcane health seedling ineffective tillering nursery Download PDFInfo
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- CN110506600A CN110506600A CN201910804335.1A CN201910804335A CN110506600A CN 110506600 A CN110506600 A CN 110506600A CN 201910804335 A CN201910804335 A CN 201910804335A CN 110506600 A CN110506600 A CN 110506600A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B13/00—Fertilisers produced by pyrogenic processes from phosphatic materials
- C05B13/02—Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of methods using sugarcane health seedling ineffective tillering nursery, this method is to cultivate sugar-cane tissue culture seedlings, ineffective tillering after collection sugarcane tissue-culture transplantation of seedlings in first generation cane stalk, takes the bud of ineffective tillering to carry out nursery in seedlings nursing plate, then carries out mechanical transplanting.Method of the invention efficiently utilizes the ineffective tillering of sugarcane health seedling, increases sugarcane health seedling stem, reduces the production cost of sugarcane health seedling, improves the income of sugarcane grower.
Description
Technical field
It is specifically a kind of to utilize sugarcane health seedling ineffective tillering nursery the present invention relates to breeding plant planting technology field
Method.
Background technique
Sugarcane (Saccharum officinarum) it is the important sugar crop that subtropical and tropical zones are generally planted,
It is of great significance to the sustainable development of Chinese national economy.In recent years, sugar supply in the world's is most of derives from cane suger,
And sugarcane kind sexual involution, perennial root difference and the not high sustainable development for having seriously affected China's Sugar Industry of per unit area yield.Productive tiller is
Sugarcane yield constituent element, and promoting tiller is the key that increase unit area number of productive tiller.Tiller originates from sugarcane plant master
Stem base portion do not extend the lateral bud of internode and sprout, and form indefinite root system, it is mature a for eventually disengaging from stem and independently developing
Body.Sugarcane tiller is also divided into effective tillering and ineffective tillering, and the tiller that general early stage occurs can grow up to productive tiller substantially, then
Phase tiller is mostly invalid.Currently, tiller mechanism has become the research that plant developmental biology and Optimum population are built up
Hot spot belongs to the quantitative character of controlled by multiple genes, common tune of the character mutation by a variety of internal factors and external environment condition
Control and influence.But focus mostly on for the research of tiller effective tillering how to be promoted to inhibit ineffective tillering so far, for invalid
Tiller is studied using but few people.
Sugarcane health seedling has many advantages, such as that the speed of growth is fast, tillering ability is strong, high at stem rate, yield is high, sowing quantity is few, is
Sugarcane Superior Variety Breeding base breeds preferred approach.And reduce sugarcane health seedling production cost and the factorial production cane seedling is
The following sugarcane developing direction.Now many research is strong by the cost of reduction sugarcane health seedling tissue culture process, raising sugarcane
The survival rate of health seedling, the transplanting of sugarcane health seedling stem, sugarcane health seedling first generation seedling stem simple bud the methods of are cultivated with drop
Low sugarcane health seedling production cost, although reducing the production cost of sugarcane health seedling to a certain extent, still
There is the space further decreased.
Summary of the invention
The object of the present invention is to provide a kind of methods using sugarcane health seedling ineffective tillering nursery, and this method is effectively
The ineffective tillering of sugarcane health seedling is utilized, increases sugarcane health seedling stem, reduces the production cost of sugarcane health seedling,
Improve the income of sugarcane grower.
A method of using sugarcane health seedling ineffective tillering nursery, it is to cultivate sugar-cane tissue culture seedlings, collects sugarcane tissue-culture
Ineffective tillering after transplantation of seedlings in first generation cane stalk takes the bud of ineffective tillering to carry out nursery in seedlings nursing plate；Specific steps are such as
Under:
(1) take the stem apex of sugarcane as explant, progress induction culturing, proliferation cultivation and cultivation of taking root obtain sweet after disinfection
Sugarcane tissue-cultured seedling.Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate, is then transplanted to sugar-cane tissue culture seedlings
Small plastic shed is built in seedlings nursing plate and covers sunshade net carries out nutrition cultivation.
(2) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
80~120kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(3) when seedling it is long to 3 blades when remove sunshade net, it is long to 4 blades when can transplant it is first right before crop field, transplanting
The blade tip of Sugarcane Seedlings is trimmed, and cuts off the 1/3~1/2 of blade, Sugarcane Seedlings are removed loading large bamboo or wicker basket with soil from seed plate,
And load onto the machinery of sowing sugarcane seedling, by 50~55 ㎝ spacing in the rows in field planting, density is 1000~1100 plants/acre, transplanting it is same
When machine automatization to be transplanted to crop field Sugarcane Seedlings water, transplant post-tensioning on Drip irrigation bag.
(4) after transplanting 10~15 days, sugarcane is applied to sugarcane and promotees 400~600g/ of tillering fertilizer mus, application is preceding thin with 2kg by it
Soil mixes, and is then uniformly sprinkling upon in row.When sugarcane seedling grows tillering seedling, cuts stem and transplanted.To the tillering seedling left
Sugarcane is applied again and promotees 300~500g/ of tillering fertilizer mus, it is mixed with 1kg fine earth before application, is then uniformly sprinkling upon in row.
(5) when harvesting first generation cane stalk, the cane stalk 5~10mm that buries is cut, band mud takes out, and then backfills
Thin mud soil is stayed overnight root；The ineffective tillering for not meeting sugarcane health seedling stem standard is filtered out, and is collected and is cut into 2~4cm
Long stem-segment with single bud.
(6) stem-segment with single bud clear water is impregnated 3~4 hours, pulls draining out and be placed on shady place and dries surface moisture.It uses again
Carbendazim, which is watered, is made 400~600 times of dilutions, and every 100mL dilution adds 8~12g imidacloprid mixture and obtains thimerosal,
Stem-segment with single bud is put into thimerosal and is impregnated 20~30 minutes, is pulled out draining after sterilizing and dries surface moisture.Again will
Stem-segment with single bud impregnates 8~12 minutes in 200mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
(7) Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate.Then above-mentioned stem-segment with single bud is pressed
Bud is implanted into installs in the seedlings nursing plate of Nutrition Soil upward, and seedlings nursing plate is closely arranged, builds Small plastic shed above it.When temperature is low
Plastic film wind-proof warming is covered on Small plastic shed when 25 DEG C, and black sunshade is covered on Small plastic shed when temperature is higher than 25 DEG C
Cloth sunshade.
(8) plastic film or puggaree are removed when sugarcane seedling length to 3 blades, starts to apply fertilizer for the first time, selection sulphur
Base composite fertilizer, by its 1:(500~600 in mass ratio) it is dissolved in spraying seedling after water or pouring and apply, it poured and applies every 5~6 days later
Once.
(9) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
80~120kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(10) crop field can be transplanted when sugarcane seedling length to 4~5 blades；Transplanting before first to the blade tip of sugarcane seedling into
Row trimming, cuts off the 1/3~1/2 of blade.By sugarcane seedling, band soil removes loading large bamboo or wicker basket from seed plate, and loads onto sowing sugarcane seedling
Machinery, by 50~55 ㎝ spacing in the rows in field planting, density is 1000~1100 plants/acre, and machine automatization is to transplanting while transplanting
Sugarcane seedling to crop field waters, and transplants Drip irrigation bag on post-tensioning.
The sugarcane seedling can be used for planting sugarcane field reseed or ratoon sugarcane reseed.
The organic fertilizer is that 80~85:1:1 is mixed to get with mass ratio by sugarcane fertilizer special for organic, Fipronil, imidaclothiz.
Sugarcane fertilizer special for organic You Ji Zhi≤55%, nitrogenous, phosphorus, potassium Zong Liang≤30%, and it is added to vegetable active tune
Save substance humic acid, EM bacterium and middle microelements of calcium, magnesium, sulphur, boron, zinc.
The sugarcane promotees tillering fertilizer and is made of following raw material by mass fraction: 5~10 parts of amino acid primary liquid, calcium magnesium phosphate
20~50 parts, 10~15 parts of EDTA, 15~20 parts of zinc sulfate, 2~3 parts of iron chloride, 4~10 parts of borax, uniconazole P 0.2~2
Part, 2~3 parts of manganese sulfate, 90~110 parts of bentonite.
The Nutrition Soil is by seedling medium, river sand and trichoderma harzianum T-22 with (6~8): (2~3): 0.1 volume
It is made than being uniformly mixed, wherein organic matter+Fu Zhi Suan≤55% in seedling medium.
The beneficial effects of the present invention are:
Since sugarcane health seedling has the characteristics that the speed of growth is fast, tillering ability is strong, sugarcane health seedling transplanting can divide behind crop field
Tiller goes out very more tillers, and the tiller that a part early stage occurs can grow up to productive tiller, and cannot grow there are also a big chunk tiller
Then become ineffective tillering at productive tiller.These ineffective tillerings are disposed during sugarcane production needs to consume a large amount of people
Power, and be difficult to judge whether the tiller is ineffective tillering in early stage people, in addition the strong tillering ability of sugarcane health seedling, in vain
Tiller is new in addition to president again, so the ineffective tillering now for sugarcane is inhibited except through cultivation technique and management
Outside, generally without manually cutting down, still there are many ineffective tillerings when harvesting sugarcane stem, one plant of sugarcane just has 0-25 root invalid
Tiller, these ineffective tillerings do not meet sugarcane health seedling stem standard again, and sugarcane grower does not adopt generally when harvesting sugarcane health seedling stem
These ineffective tillerings are received, root of staying overnight, which continues length, can not grow up to productive tiller, finally, these ineffective tillerings often waste processed
Fall.Because these ineffective tillerings are the longer first generation sugarcane stems of sugarcane health seedling, there is good kind of property.The present invention uses
Sugarcane health seedling plants out sugarcane through implantation methods of the invention, and midway does not cut ineffective tillering seedling, in harvesting first generation sugarcane
When stem, the ineffective tillering for not meeting sugarcane health seedling stem standard is uniformly collected for nursery, the collection side of ineffective tillering
Just, it solves the problems, such as to be collected separately and needs to consume a large amount of manpowers, while the ineffective tillering of this part also being made to obtain sufficient benefit
With saving sugarcane kind, avoid the waste of resource.
Sugar-cane tissue culture seedlings are first transplanted to seedlings nursing plate in method of the invention and carry out nutrition cultivation, allow it in nutrition cultivation
The environment for adapting to grown in field improves the survival rate of tissue-cultured seedling, while can adapt to mechanical planting through overnutrition cultivation and move
It plants, realizes batch production transplanting plantation, reduce the investment of manpower, improve transplanting efficiency.It deep ploughs before transplanting crop field to soil
And organic fertilizer is applied as base manure, mulch water conservation suppression grass is covered, sugarcane production and tiller are promoted.It is right after transplanting 10~15 days
Sugarcane applies sugarcane and promotees tillering fertilizer, and sugarcane can be promoted to do sth. in advance tiller, increases effective tillering.Stem shifting is cut when growing tillering seedling
It plants, the double breeding of sugarcane health seedling not only may be implemented, but also eliminate the apical dominance of former sugarcane plant, promote former sugarcane planting
The tillering seedling fast-growth of strain.It applies sugarcane again after cutting off stem and promotees tillering fertilizer, promote the further tiller of tiller more out
Tiller improves the quantity of cane stalk.
The present invention collects the ineffective tillering in sugarcane health seedling first generation sugarcane stem, is cut into stem-segment with single bud and does kind of a training
Sugarcane seedling is educated, plays the role of turning waste into wealth, increases sugarcane health seedling stem, reduces being produced into for sugarcane health seedling
This.The present invention first carries out disinfection ineffective tillering stem-segment with single bud sterilization, removes the virus in stem-segment with single bud, while improving simple bud
The ability of stem section disease and insect resistance.200mg/L Ethephon Seed Soaking is used after sterilizing again, improves bud ratio, the sugarcane of stem-segment with single bud
The tillering capacity of seedling.The present invention selects S-based compound fertilizer when to seedling top dressing in seedlings nursing plate, and S-based compound fertilizer does not burn root and do not burn
Seedling, containing a large amount of element sulphur, good absorption effect does not easily cause the bad phenomenons such as soil hardening, salinization, alkalization.The present invention makees
It arranges in pairs or groups to obtain by sugarcane fertilizer special for organic, Fipronil, imidaclothiz for the organic fertilizer of base manure, sugarcane fertilizer special for organic nutritional ingredient is rich
Fipronil, imidaclothiz is added to the targeted facilitation of the growth of sugarcane in richness, can effectively prevention and control sugarcane moth borer, woolly aphid, and
It is simultaneous to control the pests such as chafer, and the desinsection lasting period up to 2~3 months, efficacy stability, persistently.Sugarcane promotees to contain ammonia in tillering fertilizer
Each metal ion species can be complexed in base acid stoste and EDTA, EDTA, make its generation be easier to plant absorption and the complex compound utilized,
Sugarcane is improved to the absorption rate of the elements such as calcium, magnesium, iron, manganese, zinc.Amino acid primary liquid contains 17 kinds of amino acid, and content is up to
25%, nitrogenous 15% is the material base of life with upper amino acid, it is the nutriment of animals and plants common need；Amino acid primary liquid
Contained amino acid classes are complete, can be utilized by microbial bacteria, promote the breeding of Soil Microorganism bacterium；And amino acid
Stoste also has the function of that strength takes root, promotes health of root prosperity, sturdy stem, activating soil, adjusts soil acidity or alkalinity.In
Joined trichoderma harzianum T-22 in the Nutrition Soil of nursery, trichoderma harzianum T-22 as a kind of biocontrol microorganisms can be used to prevent by
Phytopathy caused by the pathogens such as pythium spp, Rhizoctonia solani Kuhn, sickle-like bacteria, bread mold, column spore be mould, sclerotinite, Sclerotium rolfsii
Evil.Trichoderma harzianum T-22 grows and is formed " protective cover " in plant rhizosphere, to prevent infecting for root disease fungus；And energy
Secretase and antibiotic agents decompose the cell wall of disease fungus.Trichoderma harzianum T-22, can be in sugarcane seedling root growth
Significantly improve the disease-resistant performance of sugarcane.
Present invention firstly provides a kind of method using sugarcane health seedling ineffective tillering nursery, this method is rationally utilized
The ineffective tillering of sugarcane health seedling, reduces the seedling cost of sugarcane health seedling, increases sugarcane health seedling stem, improves
The income of sugarcane grower.And in entire plantation and seedling raising process, work is realized in collection of transplanting, ineffective tillering of sugarcane seedling etc.
Factory, mechanization degree are high, and manpower consumption leads lower, and cost of labor is low.
Detailed description of the invention
Fig. 1,2,3,4 are the first generation sugarcane plant that sugarcane health seedling (sugar-cane tissue culture seedlings) kind comes out, and each sweet
Sugarcane plant all has an ineffective tillering, ineffective tillering or lives or dead.
Specific embodiment
For the more detailed introduction present invention, below with reference to embodiment, the present invention will be further described.
Embodiment 1
A method of it is to cultivate sugar-cane tissue culture seedlings using sugarcane health seedling ineffective tillering nursery, collects sugar-cane tissue culture seedlings and move
Ineffective tillering after cultivation in first generation cane stalk takes the bud of ineffective tillering to carry out nursery in seedlings nursing plate；Specific step is as follows:
(1) take the stem apex of sugarcane as explant, progress induction culturing, proliferation cultivation and cultivation of taking root obtain sweet after disinfection
Sugarcane tissue-cultured seedling.Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate, is then transplanted to sugar-cane tissue culture seedlings
Small plastic shed is built in seedlings nursing plate and covers sunshade net carries out nutrition cultivation.
(2) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
100kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(3) when seedling it is long to 3 blades when remove sunshade net, it is long to 4 blades when can transplant it is first right before crop field, transplanting
The blade tip of Sugarcane Seedlings is trimmed, and cuts off the 1/3 of blade, Sugarcane Seedlings are removed loading large bamboo or wicker basket with soil from seed plate, and fill
The machinery of upper sowing sugarcane seedling, by 50~55 ㎝ spacing in the rows in field planting, density is 1050 plants/acre, machine automatization while transplanting
It waters to the Sugarcane Seedlings for being transplanted to crop field, transplants Drip irrigation bag on post-tensioning.
(4) after transplanting 12 days, sugarcane is applied to sugarcane and promotees tillering fertilizer 500g/ mus, mixed it with 2kg fine earth before application, so
It is uniformly sprinkling upon in row afterwards.When sugarcane seedling grows tillering seedling, cuts stem and transplanted.Sugarcane is applied again to the tillering seedling left
Promote tillering fertilizer 400g/ mus, it is mixed with 1kg fine earth before application, is then uniformly sprinkling upon in row.
(5) when harvesting first generation cane stalk, the cane stalk 5~10mm that buries is cut, band mud takes out, and then backfills
Thin mud soil is stayed overnight root；The ineffective tillering for not meeting sugarcane health seedling stem standard is filtered out, and is collected and is cut into 2~4cm
Long stem-segment with single bud.
(6) stem-segment with single bud clear water is impregnated 3.5 hours, pulls draining out and be placed on shady place and dries surface moisture.It uses again
Carbendazim, which is watered, is made 500 times of dilutions, and every 100mL dilution adds 10g imidacloprid mixture and obtains thimerosal, by simple bud stem
Section, which is put into thimerosal, impregnates 25 minutes, is pulled out draining after sterilizing and dries surface moisture.Again by stem-segment with single bud in
It is impregnated 10 minutes in 200mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
(7) Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate.Then above-mentioned stem-segment with single bud is pressed
Bud is implanted into installs in the seedlings nursing plate of Nutrition Soil upward, and seedlings nursing plate is closely arranged, builds Small plastic shed above it.When temperature is low
Plastic film wind-proof warming is covered on Small plastic shed when 25 DEG C, and black sunshade is covered on Small plastic shed when temperature is higher than 25 DEG C
Cloth sunshade.
(8) plastic film or puggaree are removed when sugarcane seedling length to 3 blades, starts to apply fertilizer for the first time, selection sulphur
Base composite fertilizer, its 1:550 in mass ratio is dissolved in spraying seedling after water or pouring and is applied, and was poured and is applied once every 5 days later.
(9) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
100kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(10) crop field can be transplanted when sugarcane seedling length to 4~5 blades；Transplanting before first to the blade tip of sugarcane seedling into
Row trimming, cuts off the 1/3 of blade.By sugarcane seedling, band soil removes loading large bamboo or wicker basket from seed plate, and loads onto the machinery of sowing sugarcane seedling,
By 50~55 ㎝ spacing in the rows in field planting, density is 1050 plants/acre, and machine automatization is to the sugarcane for being transplanted to crop field while transplanting
Seedling watering, transplants Drip irrigation bag on post-tensioning.
The organic fertilizer is mixed to get by sugarcane fertilizer special for organic, Fipronil, imidaclothiz with mass ratio for 82:1:1.
Sugarcane fertilizer special for organic You Ji Zhi≤55%, nitrogenous, phosphorus, potassium Zong Liang≤30%, and it is added to vegetable active tune
Save substance humic acid, EM bacterium and middle microelements of calcium, magnesium, sulphur, boron, zinc；Purchased from Yulin City Lv Tao organic compound fertilizer Co., Ltd.
The sugarcane promotees tillering fertilizer and is made of following raw material by mass fraction: 8 parts of amino acid primary liquid, calcium magnesium phosphate 35
Part, 12 parts of EDTA, 18 parts of zinc sulfate, 3 parts of iron chloride, 7 parts of borax, 1 part of uniconazole P, 2 parts of manganese sulfate, 100 parts of bentonite.
The Nutrition Soil is equal with the volume ratio mixing of 7:2.5:0.1 by seedling medium, river sand and trichoderma harzianum T-22
Even to be made, wherein acid≤55% is grown in organic matter+corruption in seedling medium, and seedling medium is purchased from Changchun Xi He peat Science and Technology Ltd..
Embodiment 2
A method of it is to cultivate sugar-cane tissue culture seedlings using sugarcane health seedling ineffective tillering nursery, collects sugar-cane tissue culture seedlings and move
Ineffective tillering after cultivation in first generation cane stalk takes the bud of ineffective tillering to carry out nursery in seedlings nursing plate；Specific step is as follows:
(1) take the stem apex of sugarcane as explant, progress induction culturing, proliferation cultivation and cultivation of taking root obtain sweet after disinfection
Sugarcane tissue-cultured seedling.Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate, is then transplanted to sugar-cane tissue culture seedlings
Small plastic shed is built in seedlings nursing plate and covers sunshade net carries out nutrition cultivation.
(2) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
80kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(3) when seedling it is long to 3 blades when remove sunshade net, it is long to 4 blades when can transplant it is first right before crop field, transplanting
The blade tip of Sugarcane Seedlings is trimmed, and cuts off the 1/2 of blade, Sugarcane Seedlings are removed loading large bamboo or wicker basket with soil from seed plate, and fill
The machinery of upper sowing sugarcane seedling, by 50~55 ㎝ spacing in the rows in field planting, density is 1000 plants/acre, machine automatization while transplanting
It waters to the Sugarcane Seedlings for being transplanted to crop field, transplants Drip irrigation bag on post-tensioning.
(4) after transplanting 10 days, sugarcane is applied to sugarcane and promotees tillering fertilizer 600g/ mus, mixed it with 2kg fine earth before application, so
It is uniformly sprinkling upon in row afterwards.When sugarcane seedling grows tillering seedling, cuts stem and transplanted.Sugarcane is applied again to the tillering seedling left
Promote tillering fertilizer 300g/ mus, it is mixed with 1kg fine earth before application, is then uniformly sprinkling upon in row.
(5) when harvesting first generation cane stalk, the cane stalk 5~10mm that buries is cut, band mud takes out, and then backfills
Thin mud soil is stayed overnight root；The ineffective tillering for not meeting sugarcane health seedling stem standard is filtered out, and is collected and is cut into 2~4cm
Long stem-segment with single bud.
(6) stem-segment with single bud clear water is impregnated 4 hours, pulls draining out and be placed on shady place and dries surface moisture.Again with more
Bacterium spirit, which is watered, is made 400 times of dilutions, and every 100mL dilution adds 8g imidacloprid mixture and obtains thimerosal, by stem-segment with single bud
It is put into thimerosal and impregnates 20 minutes, pulled out draining after sterilizing and dry surface moisture.Again by stem-segment with single bud in
It is impregnated 12 minutes in 200mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
(7) Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate.Then above-mentioned stem-segment with single bud is pressed
Bud is implanted into installs in the seedlings nursing plate of Nutrition Soil upward, and seedlings nursing plate is closely arranged, builds Small plastic shed above it.When temperature is low
Plastic film wind-proof warming is covered on Small plastic shed when 25 DEG C, and black sunshade is covered on Small plastic shed when temperature is higher than 25 DEG C
Cloth sunshade.
(8) plastic film or puggaree are removed when sugarcane seedling length to 3 blades, starts to apply fertilizer for the first time, selection sulphur
Base composite fertilizer, its 1:500 in mass ratio is dissolved in spraying seedling after water or pouring and is applied, and was poured and is applied once every 6 days later.
(9) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
80kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(10) crop field can be transplanted when sugarcane seedling length to 4~5 blades；Transplanting before first to the blade tip of sugarcane seedling into
Row trimming, cuts off the 1/2 of blade.By sugarcane seedling, band soil removes loading large bamboo or wicker basket from seed plate, and loads onto the machinery of sowing sugarcane seedling,
By 50~55 ㎝ spacing in the rows in field planting, density is 1000 plants/acre, and machine automatization is to the sugarcane for being transplanted to crop field while transplanting
Seedling watering, transplants Drip irrigation bag on post-tensioning.
The organic fertilizer is mixed to get by sugarcane fertilizer special for organic, Fipronil, imidaclothiz with mass ratio for 85:1:1.
Sugarcane fertilizer special for organic You Ji Zhi≤55%, nitrogenous, phosphorus, potassium Zong Liang≤30%, and it is added to vegetable active tune
Save substance humic acid, EM bacterium and middle microelements of calcium, magnesium, sulphur, boron, zinc；Purchased from Yulin City Lv Tao organic compound fertilizer Co., Ltd.
The sugarcane promotees tillering fertilizer and is made of following raw material by mass fraction: 5 parts of amino acid primary liquid, calcium magnesium phosphate 20
Part, 10 parts of EDTA, 15 parts of zinc sulfate, 2 parts of iron chloride, 4 parts of borax, 0.2 part of uniconazole P, 2 parts of manganese sulfate, 90 parts of bentonite.
The Nutrition Soil is to be uniformly mixed by seedling medium, river sand and trichoderma harzianum T-22 with the volume ratio of 8:3:0.1
It is made, wherein acid≤55% is grown in organic matter+corruption in seedling medium, and seedling medium is purchased from Changchun Xi He peat Science and Technology Ltd..
Embodiment 3
A method of it is to cultivate sugar-cane tissue culture seedlings using sugarcane health seedling ineffective tillering nursery, collects sugar-cane tissue culture seedlings and move
Ineffective tillering after cultivation in first generation cane stalk takes the bud of ineffective tillering to carry out nursery in seedlings nursing plate；Specific step is as follows:
(1) take the stem apex of sugarcane as explant, progress induction culturing, proliferation cultivation and cultivation of taking root obtain sweet after disinfection
Sugarcane tissue-cultured seedling.Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate, is then transplanted to sugar-cane tissue culture seedlings
Small plastic shed is built in seedlings nursing plate and covers sunshade net carries out nutrition cultivation.
(2) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
120kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(3) when seedling it is long to 3 blades when remove sunshade net, it is long to 4 blades when can transplant it is first right before crop field, transplanting
The blade tip of Sugarcane Seedlings is trimmed, and cuts off the 1/3 of blade, Sugarcane Seedlings are removed loading large bamboo or wicker basket with soil from seed plate, and fill
The machinery of upper sowing sugarcane seedling, by 50~55 ㎝ spacing in the rows in field planting, density is 1100 plants/acre, machine automatization while transplanting
It waters to the Sugarcane Seedlings for being transplanted to crop field, transplants Drip irrigation bag on post-tensioning.
(4) after transplanting 15 days, sugarcane is applied to sugarcane and promotees tillering fertilizer 400g/ mus, mixed it with 2kg fine earth before application, so
It is uniformly sprinkling upon in row afterwards.When sugarcane seedling grows tillering seedling, cuts stem and transplanted.Sugarcane is applied again to the tillering seedling left
Promote tillering fertilizer 500g/ mus, it is mixed with 1kg fine earth before application, is then uniformly sprinkling upon in row.
(5) when harvesting first generation cane stalk, the cane stalk 5~10mm that buries is cut, band mud takes out, and then backfills
Thin mud soil is stayed overnight root；The ineffective tillering for not meeting sugarcane health seedling stem standard is filtered out, and is collected and is cut into 2~4cm
Long stem-segment with single bud.
(6) stem-segment with single bud clear water is impregnated 3 hours, pulls draining out and be placed on shady place and dries surface moisture.Again with more
Bacterium spirit, which is watered, is made 600 times of dilutions, and every 100mL dilution adds 12g imidacloprid mixture and obtains thimerosal, by stem-segment with single bud
It is put into thimerosal and impregnates 30 minutes, pulled out draining after sterilizing and dry surface moisture.Again by stem-segment with single bud in
It is impregnated 8 minutes in 200mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid.
(7) Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate.Then above-mentioned stem-segment with single bud is pressed
Bud is implanted into installs in the seedlings nursing plate of Nutrition Soil upward, and seedlings nursing plate is closely arranged, builds Small plastic shed above it.When temperature is low
Plastic film wind-proof warming is covered on Small plastic shed when 25 DEG C, and black sunshade is covered on Small plastic shed when temperature is higher than 25 DEG C
Cloth sunshade.
(8) plastic film or puggaree are removed when sugarcane seedling length to 3 blades, starts to apply fertilizer for the first time, selection sulphur
Base composite fertilizer, its 1:600 in mass ratio is dissolved in spraying seedling after water or pouring and is applied, and was poured and is applied once every 5 days later.
(9) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, application has
120kg/ mus of machine fertilizer, cover fine earth, mulch in covering.
(10) crop field can be transplanted when sugarcane seedling length to 4~5 blades；Transplanting before first to the blade tip of sugarcane seedling into
Row trimming, cuts off the 1/3 of blade.By sugarcane seedling, band soil removes loading large bamboo or wicker basket from seed plate, and loads onto the machinery of sowing sugarcane seedling,
By 50~55 ㎝ spacing in the rows in field planting, density is 1100 plants/acre, and machine automatization is to the sugarcane for being transplanted to crop field while transplanting
Seedling watering, transplants Drip irrigation bag on post-tensioning.
The organic fertilizer is mixed to get by sugarcane fertilizer special for organic, Fipronil, imidaclothiz with mass ratio for 80:1:1.
Sugarcane fertilizer special for organic You Ji Zhi≤55%, nitrogenous, phosphorus, potassium Zong Liang≤30%, and it is added to vegetable active tune
Save substance humic acid, EM bacterium and middle microelements of calcium, magnesium, sulphur, boron, zinc；Purchased from Yulin City Lv Tao organic compound fertilizer Co., Ltd.
The sugarcane promotees tillering fertilizer and is made of following raw material by mass fraction: 10 parts of amino acid primary liquid, calcium magnesium phosphate 50
Part, 15 parts of EDTA, 20 parts of zinc sulfate, 3 parts of iron chloride, 10 parts of borax, 2 parts of uniconazole P, 3 parts of manganese sulfate, 110 parts of bentonite.
The Nutrition Soil is to be uniformly mixed by seedling medium, river sand and trichoderma harzianum T-22 with the volume ratio of 6:2:0.1
It is made, wherein acid≤55% is grown in organic matter+corruption in seedling medium, and seedling medium is purchased from Changchun Xi He peat Science and Technology Ltd..
The tillering ability of sugarcane health seedling is very strong, and one plant of sugarcane just has 0-25 root ineffective tillering, even more, sees Fig. 1-4；
Some ineffective tillerings can be dead with the growth of sugarcane plant, sees Fig. 3；There are also ineffective tillerings to grow always, adopts
Also exist when receiving sugarcane kind, sees Fig. 4.The present invention is when collecting harvesting sugarcane kind also in the ineffective tillering grown, and uses it to be educated
Kind, although these ineffective tillerings may only have 1-5 sugarcane section, it is with extraordinary kind of property, with sugarcane health seedling stem
Kind property is similar, can be used to breeding.
Claims (4)
1. a kind of method using sugarcane health seedling ineffective tillering nursery, which is characterized in that the method is to cultivate sugarcane group
Train seedling, collect the ineffective tillering after sugarcane tissue-culture transplantation of seedlings in first generation cane stalk, take the bud of ineffective tillering in seedlings nursing plate into
Row nursery；Specific step is as follows:
(1) take the stem apex of sugarcane as explant, progress induction culturing, proliferation cultivation and cultivation of taking root obtain sweet after disinfection
Sugarcane tissue-cultured seedling, Nutrition Soil is fitted into seedlings nursing plate, and Nutrition Soil flushes the upper surface of seedlings nursing plate, is then transplanted to sugar-cane tissue culture seedlings
Small plastic shed is built in seedlings nursing plate and covers sunshade net carries out nutrition cultivation；
(2) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, apply organic fertilizer
80~120kg/ mus, cover fine earth, mulch in covering；
(3) when seedling it is long to 3 blades when remove sunshade net, it is long to 4 blades when can transplant crop field；First to sugarcane before transplanting
The blade tip of seedling is trimmed, and the 1/3~1/2 of blade is cut off；By Sugarcane Seedlings, band soil removes loading large bamboo or wicker basket from seed plate, and fills
The machinery of upper sowing sugarcane seedling, by 50~55 ㎝ spacing in the rows in field planting, density is 1000~1100 plants/acre, machine while transplanting
Tool automatically waters to the Sugarcane Seedlings for being transplanted to crop field, transplants Drip irrigation bag on post-tensioning；
(4) after transplanting 10~15 days, sugarcane is applied to sugarcane and promotees 400~600g/ of tillering fertilizer mus, mixes it with 2kg fine earth before application
It is even, it is then uniformly sprinkling upon in row；When sugarcane seedling grows tillering seedling, cuts stem and transplanted；Again to the tillering seedling left
It applies sugarcane and promotees 300~500g/ of tillering fertilizer mus, it is mixed with 1kg fine earth before application, is then uniformly sprinkling upon in row；
(5) when harvesting first generation cane stalk, the cane stalk 5~10mm that buries is cut, band mud takes out, and then backfills thin mud
Soil is stayed overnight root；The ineffective tillering for not meeting sugarcane health seedling stem standard is filtered out, and is collected and is cut into 2~4cm's long
Stem-segment with single bud；
(6) stem-segment with single bud clear water is impregnated 3~4 hours, pulls draining out and be placed on shady place and dries surface moisture；Again with more bacterium
Spirit, which is watered, is made 400~600 times of dilutions, and every 100mL dilution adds 8~12g imidacloprid mixture and obtains thimerosal, will be single
Leaf stem section, which is put into thimerosal, to be impregnated 20~30 minutes, is pulled out draining after sterilizing and dries surface moisture；Again by simple bud
Stem section is impregnated 8-12 minutes in 200mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid；
(7) Nutrition Soil is fitted into seedlings nursing plate, Nutrition Soil flushes the upper surface of seedlings nursing plate；Then above-mentioned stem-segment with single bud is pressed into bud court
It is upper to be implanted into the seedlings nursing plate for installing Nutrition Soil, seedlings nursing plate is closely arranged, builds Small plastic shed above it, when temperature is lower than 25
DEG C when plastic film wind-proof warming is covered on Small plastic shed, the screening of black puggaree cover on Small plastic shed when temperature is higher than 25 DEG C
Sun；
(8) plastic film or puggaree are removed when sugarcane seedling length to 3 blades, starts to apply fertilizer for the first time, selection sulfenyl is multiple
Hefei, by its 1:(500~600 in mass ratio) be dissolved in spraying seedling after water or pouring and apply, poured later every 5~6 days apply it is primary；
(9) 45~55cm of deep ploughing first is carried out to soil before transplanting, opens kind of a planting ditch by 1.2m line-spacing after leveling land, apply organic fertilizer
80~120kg/ mus, cover fine earth, mulch in covering；
(10) crop field can be transplanted when sugarcane seedling length to 4~5 blades；First the blade tip of sugarcane seedling is repaired before transplanting
It cuts, cuts off the 1/3~1/2 of blade；By sugarcane seedling, band soil removes loading large bamboo or wicker basket from seed plate, and loads onto the machine of sowing sugarcane seedling
Tool, by 50~55 ㎝ spacing in the rows in field planting, density is 1000~1100 plants/acre, and machine automatization is to being transplanted to while transplanting
The sugarcane seedling in crop field waters, and transplants Drip irrigation bag on post-tensioning.
2. utilizing the method for sugarcane health seedling ineffective tillering nursery according to claim 1, which is characterized in that described organic
Fertilizer is that 80~85:1:1 is mixed to get with mass ratio by sugarcane fertilizer special for organic, Fipronil, imidaclothiz；The sugarcane promotees tillering fertilizer
It is made of following raw material by mass fraction: 5~10 parts of amino acid primary liquid, 20~50 parts of calcium magnesium phosphate, 10~15 parts of EDTA,
15~20 parts of zinc sulfate, 2~3 parts of iron chloride, 4~10 parts of borax, 0.2~2 part of uniconazole P, 2~3 parts of manganese sulfate, bentonite 90
~110 parts.
3. utilizing the method for sugarcane health seedling ineffective tillering nursery according to claim 2, which is characterized in that the sugarcane
Fertilizer special for organic You Ji Zhi≤55%, nitrogenous, phosphorus, potassium Zong Liang≤30%, and it is added to vegetable active Auto-regulator humic acid, EM bacterium
With middle microelements of calcium, magnesium, sulphur, boron, zinc.
4. utilizing the method for sugarcane health seedling ineffective tillering nursery according to claim 1, which is characterized in that the nutrition
Soil is by seedling medium, river sand and trichoderma harzianum T-22 with (6~8): (2~3): 0.1 volume ratio, which is uniformly mixed, to be made,
Organic matter+Fu Zhi Suan≤55% in middle seedling medium.
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| CN111727687A (en) * | 2020-06-30 | 2020-10-02 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane transplanted seedlings |
| CN112166990A (en) * | 2020-09-23 | 2021-01-05 | 广西南亚热带农业科学研究所 | Sugarcane seedling cultivation method |
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| CN113439559A (en) * | 2021-08-05 | 2021-09-28 | 广西壮族自治区农业科学院 | Method for directly producing raw sugarcane by using sugarcane tissue culture seedlings |
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| CN114667899A (en) * | 2022-03-07 | 2022-06-28 | 云南省农业科学院甘蔗研究所 | Late planting close planting multi-tillering seed production method for healthy sugarcane seedlings |
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| CN114667899A (en) * | 2022-03-07 | 2022-06-28 | 云南省农业科学院甘蔗研究所 | Late planting close planting multi-tillering seed production method for healthy sugarcane seedlings |
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